Yfunc lets you browse current and predicted gene functions in yeast

Enter a GO term ID (example: GO:0003723):
Enter a yeast gene name to browse (example:AIR1):

Prediction of GO:0000386 "second spliceosomal transesterification activity"

GO branch: MF
Number of currently annotated genes: 3

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest TAP:161Giaever:fenpropimorph_1.17uMHarbison:RPH1_SMPF07717PS50126PF04408Lee:GCR2PF00575IPR002464IPR007502 S000001794PRP16YKR086WverifiedRNA helicase in the DEAH-box family involved in the secondcatalytic step of splicing, exhibitsATP-dependent RNA unwinding activity 0.032230.55735&radic&radic&radic&radic S000004106MSL5YLR116WverifiedComponent of the commitment complex, which defines the firststep in the splicing pathway; essential proteinthat interacts with Mud2p and Prp40p, forming abridge between the intron ends; also involvedin nuclear retention of pre-mRNA 0.031420.55161 S000003767SUI2YJR007WverifiedAlpha subunit of the translation initiation factor eIF2,involved in the identification of the startcodon; phosphorylation of Ser51 is required forregulation of translation by inhibiting theexchange of GDP for GTP 0.018760.44395&radic&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p 0.018110.43619 S000002334AIR2YDL175CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p; may regulatemethylation of Npl3p, which modulates Npl3pfunction in mRNA processing and export; hassimilarity to Air1p 0.017870.43619 S000005199GIS2YNL255CverifiedProtein with seven cysteine-rich CCHC zinc-finger motifs,similar to human CNBP, proposed to be involvedin the RAS/cAMP signaling pathway 0.017160.42932 S000004842RRP5YMR229CverifiedProtein required for the synthesis of both 18S and 5.8S rRNA;C-terminal region is crucial for the formationof 18S rRNA and N-terminal region is requiredfor the 5.8S rRNA; component of small ribosomalsubunit (SSU) processosome 0.016790.42548&radic&radic S000002812RPB7YDR404CverifiedRNA polymerase II subunit B16; forms two subunit dissociablecomplex with Rpb4p 0.015380.40745&radic S000003088PRP43YGL120CverifiedRNA helicase in the DEAH-box family, functions in both RNApolymerase I and polymerase II transcriptmetabolism, involved in release of thelariat-intron from the spliceosome 0.010650.33768&radic&radic&radic&radic&radic S000004411YLR419WUncharacterizedPutative helicase with limited sequence similarity to human Rb protein; localized to the cytoplasm and the mitochondria; YLR419W is not an essential gene 0.010190.33021&radic&radic&radic S000005294PRP2YNR011CverifiedRNA-dependent ATPase in the DEAH-box family, required foractivation of the spliceosome before the firsttransesterification step in RNA splicing 0.010080.33021&radic&radic&radic&radic S000000939YER137CUncharacterizedHypothetical protein 0.008240.29596 S000004735ECM16YMR128WverifiedEssential DEAH-box ATP-dependent RNA helicase specific to theU3 snoRNP, predominantly nucleolar indistribution, required for 18S rRNA synthesis 0.008040.28903&radic&radic&radic&radic S000005502RRP40YOL142WverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.007860.28647&radic S000005381DIS3YOL021CverifiedNucleolar exosome component, involved in rRNA processing andRNA degradation, binds Gsp1p/Ran and enhancesthe GEF activity of Srm1p, implicated inmitotic control, homologous to the E. coliRNase R of the RNase II family 0.007040.27238&radic S000001561DHR2YKL078WverifiedPredominantly nucleolar DEAH-box RNA helicase, required for 18SrRNA synthesis 0.006790.26331&radic&radic&radic&radic S000001111RRP4YHR069CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp43p and Dis3p 0.006290.25644&radic S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif 0.005430.23436 S000006311YTH1YPR107CverifiedEssential RNA-binding component of cleavage and polyadenylationfactor, contains five zinc fingers; requiredfor pre-mRNA 3'-end processing andpolyadenylation 0.003950.19763 S000003506TAF1YGR274CverifiedTFIID subunit (145 kDa), involved in RNA polymerase IItranscription initiation, has histoneacetyltransferase activity, involved inpromoter binding and G1/S progression 0.002980.17246 S000002491TVP23YDR084CuncharacterizedIntegral membrane protein localized to late Golgi vesiclesalong with the v-SNARE Tlg2p; green fluorescentprotein (GFP)-fusion protein localizes to thecytoplasm in a punctate pattern 0.002280.14288&radic S000004331YLR339CDubiousHypothetical protein; open reading frame overlaps 5' end of essential RPP0 gene encoding a component of the ribosome 0.00220.1403&radic S000004293YLR302CDubiousHypothetical protein 0.00220.1403&radic S000002653MNN10YDR245WverifiedSubunit of a Golgi mannosyltransferase complex also containingAnp1p, Mnn9p, Mnn11p, and Hoc1p that mediateselongation of the polysaccharide mannanbackbone; membrane protein of themannosyltransferase family 0.002050.13208&radic S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.002080.13208 S000002475DOS2YDR068WverifiedProtein of unknown function, green fluorescent protein(GFP)-fusion protein localizes to the cytoplasm 0.002010.13208&radic S000002881PRP3YDR473CverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.001860.12676&radic&radic S000004333SPO77YLR341WverifiedMeiosis-specific protein of unknown function, required forspore wall formation during sporulation;dispensable for both nuclear divisions duringmeiosis 0.001750.1192&radic S000002814PDR15YDR406WverifiedATP binding cassette (ABC) transporter of the plasma membrane;general stress response factor implicated incellular detoxification; target of Pdr1p, Pdr3pand Pdr8p transcription regulators; promotercontains a PDR responsive element 0.001660.1192&radic S000001069RPN1YHR027CverifiedNon-ATPase base subunit of the 19S regulatory particle of the26S proteasome; may participate in therecognition of several ligands of theproteasome; contains a leucine-rich repeat(LRR) domain, a site for protein?proteininteractions 0.001630.11222&radic S000002243NDE2YDL085WverifiedMitochondrial external NADH dehydrogenase, catalyzes theoxidation of cytosolic NADH; Nde1p and Nde2pare involved in providing the cytosolic NADH tothe mitochondrial respiratory chain 0.001270.10478&radic S000002892VPS52YDR484WverifiedComponent of the GARP (Golgi-associated retrograde protein)complex, Vps51p-Vps52p-Vps53p-Vps54p, which isrequired for the recycling of proteins fromendosomes to the late Golgi; involved inlocalization of actin and chitin 0.001250.10342&radic S000004072SRL2YLR082CverifiedProtein of unknown function; overexpression suppresses thelethality caused by a rad53 null mutation 0.000850.08534&radic S000002474OCA6YDR067CuncharacterizedCytoplasmic protein required for replication of Brome mosaicvirus in S. cerevisiae, which is a model systemfor studying replication of positive-strand RNAviruses in their natural hosts 0.000790.08099&radic S000000155YBL059WUncharacterizedHypothetical protein 0.00080.08099&radic S000002701SSD1YDR293CverifiedProtein with a role in maintenance of cellular integrity,interacts with components of the TOR pathway;ssd1 mutant of a clinical S. cerevisiae straindisplays elevated virulence 0.000170.00636 S000002495SLU7YDR088CverifiedRNA splicing factor, required for ATP-independent portion of2nd catalytic step of spliceosomal RNAsplicing; interacts with Prp18p; contains zincknuckle domain &radic0.000130.00447&radic&radic S000004107CLF1YLR117CverifiedEssential splicesome assembly factor; contains multipletetratricopeptide repeat (TPR) protein-bindingmotifs and interacts specifically with manyspliceosome components, may serve as a scaffoldduring splicesome assembly 0.000130.00447&radic S000001208PRP8YHR165CverifiedComponent of the U4/U6-U5 snRNP complex, involved in the secondcatalytic step of splicing 0.000120.00427&radic S000003238PRP18YGR006WverifiedSplicing factor involved in the positioning of the 3' splicesite during the second catalytic step ofsplicing, recruited to the spliceosome by Slu7p &radic0.000120.00418&radic S000002772CDC40YDR364CverifiedPre-mRNA splicing factor, important for catalytic step II ofpre-mRNA splicing and plays a role in cellcycle progression; required for DNA synthesisduring mitosis and meiosis; has WD repeats 0.000110.004 S000004826CEF1YMR213WverifiedEssential splicing factor; associated with Prp19p and thespliceosome, contains an N-terminal c-Myb DNAbinding motif necessary for cell viability butnot for Prp19p association, evolutionarilyconserved and homologous to S. pombe Cdc5p 9e-050.00361&radic S000005937SWI1YPL016WverifiedSubunit of the SWI/SNF chromatin remodeling complex, whichregulates transcription by remodelingchromosomes; required for transcription of manygenes, including ADH1, ADH2, GAL1, HO, INO1 andSUC2 9e-050.00361 S000003959PRP19YLL036CverifiedSplicing factor associated with the spliceosome; contains aU-box, a motif found in a class of ubiquitinligases 9e-050.00361&radic S000000030PRP45YAL032CverifiedProtein required for pre-mRNA splicing; associates with thespliceosome and interacts with splicing factorsPrp22p and Prp46p; orthologous to humantranscriptional coactivator SKIP and canactivate transcription of a reporter gene 9e-050.00332 S000004425CNA1YLR433CverifiedCalcineurin A; one isoform (the other is CMP2) of the catalyticsubunit of calcineurin, aCa++/calmodulin-regulated protein phosphatasewhich regulates Crz1p (a stress-responsetranscription factor), the other calcineurinsubunit is CNB1 8e-050.00322 S000000467SHM1YBR263WverifiedMitochondrial serine hydroxymethyltransferase, involved inone-carbon metabolism 7e-050.00307 S000000974BRR2YER172CverifiedRNA-dependent ATPase RNA helicase involved in the facilitationand disruption of snRNA interactions, requiredfor disruption of U4/U6 base-pairing in nativesnRNPs to activate the spliceosome forcatalysis 7e-050.00292 S000004618SOK2YMR016CverifiedNuclear protein that plays a regulatory role in the cyclic AMP(cAMP)-dependent protein kinase (PKA) signaltransduction pathway; negatively regulatespseudohyphal differentiation; homologous toseveral transcription factors 7e-050.00292 S000003306SMD1YGR074WverifiedCore Sm protein Sm D1; part of heteroheptameric complex (withSmb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D1 7e-050.00287&radic S000002280UBP1YDL122WverifiedUbiquitin-specific protease that removes ubiquitin fromubiquitinated proteins; cleaves at the Cterminus of ubiquitin fusions irrespective oftheir size; capable of cleaving polyubiquitinchains 6e-050.00281 S000001656SNU114YKL173WverifiedGTPase component of U5 snRNP involved in mRNA splicing viaspliceosome; binds directly to U5 snRNA;proposed to be involved in conformationalchanges of the spliceosome; similarity toribosomal translocation factor EF-2 6e-050.00278&radic S000006305SNT309YPR101WverifiedComponent of NineTeen complex (NTC) containing Prp19p involvedin mRNA splicing, interacts physically andgenetically with Prp19p 6e-050.00274&radic S000003704SET2YJL168CverifiedHistone methyltransferase with a role in transcriptionalelongation, methylates a lysine residue ofhistone H3; associates with the C-terminaldomain of Rpo21p; histone methylation activityis regulated by phosphorylation status ofRpo21p 6e-050.00269 S000003770SPC1YJR010C-AverifiedSubunit of the signal peptidase complex (SPC), which cleavesthe signal sequence from proteins targeted tothe endoplasmic reticulum (ER), homolog of theSPC12 subunit of mammalian signal peptidasecomplex 6e-050.00269 S000002368CWC2YDL209CverifiedProtein involved in pre-mRNA splicing, component of a complexcontaining Cef1p; interacts with Prp19p;contains an RNA recognition motif; hassimilarity to S. pombe Cwf2p 6e-050.00269&radic S000002517FOB1YDR110WverifiedNucleolar protein required for DNA replication fork blockingand recombinational hotspot activities; bindsto the replication fork barrier site in therDNA region; related to retroviral integrases 6e-050.00268 S000001803MLP1YKR095WverifiedMyosin-like protein associated with the nuclear envelope,connects the nuclear pore complex with thenuclear interior; involved with Tel1p intelomere length control; involved with Pml1pand Pml39p in nuclear retention of unsplicedmRNAs 6e-050.00268 S000001065MYO1YHR023WverifiedType II myosin heavy chain, required for wild-type cytokinesisand cell separation; localizes to theactomyosin ring; binds to myosin light chainsMlc1p and Mlc2p through its IQ1 and IQ2 motifsrespectively 6e-050.00268 S000000805PMI40YER003CverifiedMannose-6-phosphate isomerase, catalyzes the interconversion offructose-6-P and mannose-6-P; required forearly steps in protein mannosylation 6e-050.00268 S000003464NAS6YGR232WverifiedRegulatory, non-ATPase subunit of the 26S proteasome; homologof the human oncoprotein gankyrin, whichinteracts with the retinoblastoma tumorsuppressor (Rb) and cyclin-dependent kinase 4/6 5e-050.00257 S000000991YER189WUncharacterizedHypothetical protein 5e-050.00257 S000003130SUT1YGL162WverifiedTranscription factor of the Zn[II]2Cys6 family involved insterol uptake; involved in induction of hypoxicgene expression 5e-050.00256 S000000815PRP22YER013WverifiedDEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase,associates with lariat intermediates before thesecond catalytic step of splicing; mediatesATP-dependent mRNA release from the spliceosomeand unwinds RNA duplexes &radic5e-050.00256&radic&radic&radic&radic&radic&radic&radic&radic S000000269ECM2YBR065CverifiedPre-mRNA splicing factor, facilitates the cooperative formationof U2/U6 helix II in association with stem IIin the spliceosome, function may be regulatedby Slu7p 5e-050.00256&radic S000006211REC8YPR007CverifiedMeiosis-specific component of sister chromatid cohesioncomplex; maintains cohesion between sisterchromatids during meiosis I; maintains cohesionbetween centromeres of sister chromatids untilmeiosis II; homolog of S. pombe Rec8p 5e-050.00256 S000003806SSC1YJR045CverifiedMitochondrial matrix ATPase that is a subunit of thepresequence translocase-associated proteinimport motor (PAM); involved in proteintranslocation into the matrix and proteinfolding; member of the heat shock protein 70(HSP70) family 5e-050.00245 S000028561YLR154C-GUncharacterizedPutative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF is contained within RDN25-2 and RDN37-2 5e-050.00245 S000003180VAM7YGL212WverifiedComponent of the vacuole SNARE complex involved in vacuolarmorphogenesis; SNAP-25 homolog; functions witha syntaxin homolog Vam3p in vacuolar proteintrafficking 5e-050.00245 S000003183CLG1YGL215WverifiedCyclin-like protein that interacts with Pho85p; has sequencesimilarity to G1 cyclins PCL1 and PCL2 5e-050.00245 S000005758MGE1YOR232WverifiedProtein of the mitochondrial matrix involved in protein importinto mitochondria; acts as a cochaperone and anucleotide release factor for Ssc1p; homolog ofE. coli GrpE 5e-050.00245 S000006245TIF5YPR041WverifiedTranslation initiation factor eIF-5; N-terminal domainfunctions as a GTPase-activating protein tomediate hydrolysis of ribosome-bound GTP;C-terminal domain is the core of ribosomalpreinitiation complex formation 5e-050.00245 S000002675CIA1YDR267CverifiedEssential protein involved in assembly of cytosolic and nucleariron-sulfur proteins 5e-050.00245 S000001809SIR1YKR101WverifiedProtein involved in repression of transcription at the silentmating-type loci HML and HMR; recruitment tosilent chromatin requires interactions withOrc1p and with Sir4p, through a common Sir1pdomain; binds to centromeric chromatin 5e-050.00245 S000005014TOM7YNL070WverifiedComponent of the TOM (translocase of outer membrane) complexresponsible for recognition and initial importsteps for all mitochondrially directedproteins; promotes assembly and stability ofthe TOM complex 5e-050.00245 S000006203PAU22YPL282CuncharacterizedHypothetical protein 5e-050.00245 S000000027MYO4YAL029CverifiedOne of two type V myosin motors (along with MYO2) involved inactin-based transport of cargos; required formRNA transport, including ASH1 mRNA, andfacilitating the growth and movement of ERtubules into the growing bud along with She3p 5e-050.00245 S000003412RNR4YGR180CverifiedRibonucleotide-diphosphate reductase (RNR), small subunit; theRNR complex catalyzes the rate-limiting step indNTP synthesis and is regulated by DNAreplication and DNA damage checkpoint pathwaysvia localization of the small subunits 5e-050.00245 S000003963VPS13YLL040CverifiedProtein of unknown function; heterooligomeric or homooligomericcomplex; peripherally associated withmembranes; homologous to human COH1; involvedin sporulation, vacuolar protein sorting andprotein-Golgi retention 5e-050.00245 S000005083RLR1YNL139CverifiedSubunit of the THO complex, which is required for efficienttranscription elongation and involved intranscriptional elongation-associatedrecombination; required for LacZ RNA expressionfrom certain plasmids 5e-050.00245 S000004969YNL024CUncharacterizedPutative protein of unknown function with seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL024C is not an essential gene 5e-050.00244 S000005611OST3YOR085WverifiedGamma subunit of the oligosaccharyltransferase complex of theER lumen, which catalyzes asparagine-linkedglycosylation of newly synthesized proteins;Ost3p is important for N-glycosylation of asubset of proteins 5e-050.00244 S000003387CYS4YGR155WverifiedCystathionine beta-synthase, catalyzes the synthesis ofcystathionine from serine and homocysteine, thefirst committed step in cysteine biosynthesis 5e-050.00244 S000000094PHO11YAR071WverifiedOne of three repressible acid phosphatases, a glycoprotein thatis transported to the cell surface by thesecretory pathway; induced by phosphatestarvation and coordinately regulated by PHO4and PHO2 5e-050.00244 S000003213RTF1YGL244WverifiedSubunit of the RNA polymerase II-associated Paf1 complex;directly or indirectly regulates DNA-bindingproperties of Spt15p and relative activities ofdifferent TATA elements; involved in telomeremaintenance 5e-050.00244 S000003316MRP13YGR084CverifiedMitochondrial ribosomal protein of the small subunit 5e-050.00244 S000001591SLD2YKL108WverifiedProtein required for DNA replication, phosphorylated in S phaseby S-phase cyclin-dependent kinases (Cdks),phosphorylation is essential for DNAreplication and for complex formation withDpb11p; potential Cdc28p substrate 5e-050.00244 S000000001TFC3YAL001CverifiedLargest of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauB domain of TFIIIC that binds DNA at theBoxB promoter sites of tRNA and similar genes;cooperates with Tfc6p in DNA binding 5e-050.00244 S000003729YJL193WUncharacterizedPutative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect 5e-050.00241 S000004447YLR455WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR455W is not an essential gene 5e-050.00241 S000006161HSP82YPL240CverifiedCytoplasmic chaperone (Hsp90 family) required for pheromonesignaling and negative regulation of Hsf1p;docks with the mitochondrial import receptorTom70p for preprotein delivery; interacts withco-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p 5e-050.00241 S000005676MRPL23YOR150WverifiedMitochondrial ribosomal protein of the large subunit 5e-050.00241 S000001209CDC23YHR166CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition 5e-050.00241 S000005035NST1YNL091WverifiedProtein of unknown function, mediates sensitivity to saltstress; interacts physically with the splicingfactor Msl1p and also displays geneticinteraction with MSL1 5e-050.00241 S000002939YDR531WUncharacterizedPantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway leading to coenzyme A1 5e-050.00241 S000001692STE6YKL209CverifiedATP-binding cassette (ABC) transporter required for the exportof a-factor, catalyzes ATP hydrolysis coupledto a-factor transport, contains 12transmembrane domains and two ATP bindingdomains, expressed only in MATa cells 5e-050.00241 S000004089ICT1YLR099CverifiedProtein of unknown function, null mutation leads to an increasein sensitivity to Calcofluor white; expressionof the gene is induced in the presence ofisooctane 5e-050.00241 S000004036YLR046CUncharacterizedPutative membrane protein, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance 5e-050.00241 S000004762NUP53YMR153WverifiedSubunit of the nuclear pore complex (NPC), interacts withkaryopherin Kap121p or with Nup170p viaoverlapping regions of Nup53p, involved inactivation of the spindle checkpoint mediatedby the Mad1p-Mad2p complex 5e-050.00241 S000002692DPP1YDR284CverifiedDiacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulatedvacuolar membrane-associated lipid phosphatase,dephosphorylates DGPP to phosphatidate (PA) andPi, then PA to diacylglycerol; involved inlipid signaling and cell metabolism 5e-050.00241 S000005152YNL208WUncharacterizedHypothetical protein 5e-050.00241 S000000113PEP1YBL017CverifiedType I transmembrane sorting receptor for multiple vacuolarhydrolases; cycles between the late-Golgi andprevacuolar endosome-like compartments 5e-050.00241 S000005347YNR064CVerifiedEpoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides 5e-050.00241 S000000826YAT2YER024WverifiedCarnitine acetyltransferase; has similarity to Yat1p, which isa carnitine acetyltransferase associated withthe mitochondrial outer membrane 5e-050.00241 S000000443YBR239CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR239C is not an essential gene 5e-050.00236 S000000033FUN12YAL035WverifiedGTPase, required for general translation initiation bypromoting Met-tRNAiMet binding to ribosomes andribosomal subunit joining; homolog of bacterialIF2 5e-050.00236 S000005832YOR305WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; YOR305W is not an essential gene 5e-050.00236 S000000283RPG1YBR079CverifiedSubunit of the core complex of translation initiation factor3(eIF3), essential for translation; part of asubcomplex (Prt1p-Rpg1p-Nip1p) that stimulatesbinding of mRNA and tRNA(i)Met to ribosomes 5e-050.00236 S000002726MCM21YDR318WverifiedProtein involved in minichromosome maintenance; component ofthe COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p)that bridges kinetochore subunits that are incontact with centromeric DNA and the subunitsbound to microtubules 5e-050.00236 S000004954IDP3YNL009WverifiedPeroxisomal NADP-dependent isocitrate dehydrogenase, catalyzesoxidation of isocitrate to alpha-ketoglutaratewith the formation of NADP(H+), required forgrowth on unsaturated fatty acids 5e-050.00236 S000001473LYS1YIR034CverifiedSaccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzesthe conversion of saccharopine to L-lysine,which is the final step in the lysinebiosynthesis pathway 5e-050.00236 S000002717GIC2YDR309CverifiedProtein of unknown function involved in initiation of buddingand cellular polarization, interacts withCdc42p via the Cdc42/Rac-interactive binding(CRIB) domain 5e-050.00236 S000028651YHR213W-AUncharacterizedIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 5e-050.00236 S000004565ARG81YML099CverifiedZinc-finger transcription factor of the Zn(2)-Cys(6) binuclearcluster domain type, involved in the regulationof arginine-responsive genes; acts with Arg80pand Arg82p 5e-050.00236 S000005636TFC7YOR110WverifiedOne of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauA globular domain of TFIIIC that binds DNAat the BoxA promoter sites of tRNA and similargenes 5e-050.00236 S000001938YFR042WUncharacterizedPutative protein of unknown function; required for cell viability 5e-050.00236 S000003309PEX8YGR077CverifiedIntraperoxisomal organizer of the peroxisomal import machinery,tightly associated with the lumenal face of theperoxisomal membrane, essential for peroxisomebiogenesis, binds PTS1-signal receptor Pex5p 5e-050.00236 S000004351ADE13YLR359WverifiedAdenylosuccinate lyase, catalyzes two steps in the 'de novo'purine nucleotide biosynthetic pathway 5e-050.00236 S000002965SMX2YFL017W-AverifiedCore Sm protein Sm G; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm G 5e-050.00236&radic S000003433YGR201CUncharacterizedPutative protein of unknown function 5e-050.00236 S000004077CSF1YLR087CverifiedProtein required for fermentation at low temperature; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 5e-050.00236 S000001756NAP1YKR048CverifiedProtein that interacts with mitotic cyclin Clb2p; required forthe regulation of microtubule dynamics duringmitosis; controls bud morphogenesis; involvedin the transport of H2A and H2B histones to thenucleus 5e-050.00236 S000000452HIS7YBR248CverifiedImidazole glycerol phosphate synthase (glutamineamidotransferase:cyclase), catalyzes the fifthand sixth steps of histidine biosynthesis andalso produces 5-aminoimidazole-4-carboxamideribotide (AICAR), a purine precursor 5e-050.00236&radic S000003665TRK1YJL129CverifiedComponent of the Trk1p-Trk2p potassium transport system; 180kDa high affinity potassium transporter;phosphorylated in vivo and interacts physicallywith the phosphatase Ppz1p, suggesting Trk1pacitivy is regulated by phosphorylation 5e-050.00236 S000003128YGL160WUncharacterizedPutative protein of uknown function with sequence similarity to iron/copper reductases (FRE1-8), possibly involved in iron homeostasis 5e-050.00236 S000001147YPT35YHR105WverifiedEndosomal protein of unknown function that contains a phox (PX)homology domain and binds to bothphosphatidylinositol-3-phosphate (PtdIns(3)P)and proteins involved in ER-Golgi or vesiculartransport 5e-050.00236 S000004737YMR130WUncharacterizedHypothetical protein 5e-050.00236 S000003342YGR110WUncharacterizedPutative protein of unknown function; transcription is increased in response to genotoxic stress 5e-050.00236 S000000924GLO3YER122CverifiedADP-ribosylation factor GTPase activating protein (ARF GAP),involved in ER-Golgi transport; sharesfunctional similarity with Gcs1p 5e-050.00236 S000005256RFA2YNL312WverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 5e-050.00236 S000006191MDL2YPL270WverifiedHalf-type ATP-binding cassette (ABC) transporter of the innermitochondrial membrane 5e-050.00236 S000004387COX8YLR395CverifiedSubunit VIII of cytochrome c oxidase, which is the terminalmember of the mitochondrial inner membraneelectron transport chain 5e-050.00236 S000006386SMX3YPR182WverifiedCore Sm protein Sm F; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm F 5e-050.00236&radic S000002303YDL144CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene. 5e-050.00236 S000006165HUT1YPL244CverifiedProtein with a role in UDP-galactose transport to the Golgilumen, has similarity to human UDP-galactosetransporter UGTrel1, exhibits a geneticinteraction with S. cerevisiae ERO1 5e-050.00236 S000004506CAT2YML042WverifiedCarnitine acetyl-CoA transferase present in both mitochondriaand peroxisomes, transfers activated acetylgroups to carnitine to form acetylcarnitinewhich can be shuttled across membranes 5e-050.00236 S000006036BEM3YPL115CverifiedRho GTPase activating protein (RhoGAP) involved in control ofthe cytoskeleton organization; targets theessential Rho-GTPase Cdc42p, which controlsestablishment and maintenance of cell polarity,including bud-site assembly 5e-050.00236 S000001929QCR6YFR033CverifiedSubunit 6 of the ubiquinol cytochrome-c reductase complex,which is a component of the mitochondrial innermembrane electron transport chain; highlyacidic protein; required for maturation ofcytochrome c1 5e-050.00236 S000003378YGR146CUncharacterizedPutative protein of unknown function; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of a temperature sensitive hsf1 mutant 5e-050.00236 S000028740YDR524W-CUncharacterizedIdentified by SAGE 5e-050.00236 S000000446YBR242WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene 5e-050.00236 S000003503SLH1YGR271WverifiedPutative RNA helicase related to Ski2p, involved in translationinhibition of non-poly(A) mRNAs; required forrepressing propagation of dsRNA viruses 5e-050.00236 S000005915FDH1YOR388CverifiedNAD(+)-dependent formate dehydrogenase, may protect cells fromexogenous formate 5e-050.00236 S000005362IZH2YOL002CverifiedPlasma membrane protein involved in zinc metabolism andosmotin-induced apoptosis; transcriptionregulated by Zap1p, zinc and fatty acid levels;similar to mammalian adiponectins; deletionincreases sensitivity to elevated zinc 5e-050.00236 S000000662RAD18YCR066WverifiedProtein involved in postreplication repair; bindssingle-stranded DNA and has single-stranded DNAdependent ATPase activity; forms heterodimerwith Rad6p; contains RING-finger motif 5e-050.00236 S000001548YET1YKL065CverifiedEndoplasmic reticulum transmembrane protein; may interact withribosomes, based on co-purificationexperiments; homolog of human BAP31 protein 5e-050.00236 S000002498RLI1YDR091CverifiedEssential iron-sulfur protein required for ribosome biogenesisand translation initiation; facilitates bindingof a multifactor complex (MFC) of translationinitiation factors to the small ribosomalsubunit; predicted ABC family ATPase 4e-050.00232 S000000459YBR255WUncharacterizedProtein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 4e-050.00232 S000002327SFA1YDL168WverifiedBifunctional enzyme containing both alcohol dehydrogenase andglutathione-dependent formaldehydedehydrogenase activities, functions informaldehyde detoxification and formation oflong chain and complex alcohols, regulated byHog1p-Sko1p 4e-050.00232 S000003625SIP4YJL089WverifiedC6 zinc cluster transcriptional activator that binds to thecarbon source-responsive element (CSRE) ofgluconeogenic genes; involved in the positiveregulation of gluconeogenesis; regulated bySnf1p protein kinase; localized to the nucleus 4e-050.00232 S000003867ECM27YJR106WverifiedNon-essential protein of unknown function 4e-050.00232 S000005621RKI1YOR095CverifiedRibose-5-phosphate ketol-isomerase, catalyzes theinterconversion of ribose 5-phosphate andribulose 5-phosphate in the pentose phosphatepathway; participates in pyridoxinebiosynthesis 4e-050.00232 S000005834SLY41YOR307CverifiedProtein involved in ER-to-Golgi transport 4e-050.00232 S000005450MSH2YOL090WverifiedProtein that forms heterodimers with Msh3p and Msh6p that bindto DNA mismatches to initiate the mismatchrepair process; contains a Walker ATP-bindingmotif required for repair activity; Msh2p-Msh6pbinds to and hydrolyzes ATP 4e-050.00232 S000003374BTN2YGR142Wverifiedv-SNARE binding protein that facilitates specific proteinretrieval from a late endosome to the Golgi;modulates arginine uptake, possible role inmediating pH homeostasis between the vacuoleand plasma membrane H(+)-ATPase 4e-050.00232 S000003105SEC27YGL137WverifiedEssential beta'-coat protein of the COPI coatomer, involved inER-to-Golgi and Golgi-to-ER transport; containsWD40 domains that mediate cargo selectiveinteractions; 45% sequence identity tomammalian beta'-COP 4e-050.00232 S000005251MCK1YNL307CverifiedProtein serine/threonine/tyrosine (dual-specificity) kinaseinvolved in control of chromosome segregationand in regulating entry into meiosis; relatedto mammalian glycogen synthase kinases of theGSK-3 family 4e-050.00232 S000001644YKL161CVerifiedProtein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; associates with Rlm1p 4e-050.00232 S000005319YNR036CUncharacterizedPutative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli and human mitochondrial S12 ribosomal proteins 4e-050.00232 S000004203CRR1YLR213CverifiedPutative glycoside hydrolase of the spore wall envelope;required for normal spore wall assembly,possibly for cross-linking between the glucanand chitosan layers; expressed duringsporulation 4e-050.00232 S000004594PGA3YML125CuncharacterizedEssential protein required for maturation of Gas1p and Pho8p,protein trafficking; GFP-fusion proteinlocalizes to the endoplasmic reticulum; nullmutants have a cell separation defect 4e-050.00232 S000000769YEL043WUncharacterizedPredicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; contains fibronectin type III domain fold 4e-050.00232 S000002815TRS120YDR407CverifiedOne of 10 subunits of the transport protein particle (TRAPP)complex of the cis-Golgi which mediates vesicledocking and fusion; involved in endoplasmicreticulum (ER) to Golgi membrane traffic 4e-050.00232 S000001045YHR003CVerifiedProtein of unknown function, localized to the mitochondrial outer membrane 4e-050.00232 S000005985CWC27YPL064CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to S. pombeCwf27p 4e-050.00232 S000001813YKR105CUncharacterizedHypothetical protein 4e-050.00232 S000001143BIG1YHR101CverifiedIntegral membrane protein of the endoplasmic reticulum,required for normal content of cell wallbeta-1,6-glucan 4e-050.00232 S000028764YER188C-AUncharacterized 4e-050.00232 S000004038RPS0BYLR048WverifiedProtein component of the small (40S) ribosomal subunit, nearlyidentical to Rps0Ap; required for maturation of18S rRNA along with Rps0Ap; deletion of eitherRPS0 gene reduces growth rate, deletion of bothgenes is lethal 4e-050.00232 S000004707SRT1YMR101CverifiedCis-prenyltransferase involved in synthesis of long-chaindolichols (19-22 isoprene units; as opposed toRer2p which synthesizes shorter-chaindolichols); localizes to lipid bodies;transcription is induced during stationaryphase 4e-050.00232 S000001117PPE1YHR075CverifiedProtein with carboxyl methyl esterase activity that may have arole in demethylation of the phosphoproteinphosphatase catalytic subunit; also identifiedas a small subunit mitochondrial ribosomalprotein 4e-050.00232 S000001052RPL27AYHR010WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl27Bp and has similarity to ratL27 ribosomal protein 4e-050.00232 S000004381STE23YLR389CverifiedMetalloprotease involved, with homolog Axl1p, in N-terminalprocessing of pro-a-factor to the mature form;member of the insulin-degrading enzyme family 4e-050.00232 S000006127YPL206CUncharacterizedProtein that plays a role in metabolism of glycerophosphocholine to phosphatidylcholine; contains glycerophosphodiester phosphodiesterase motifs 4e-050.00232 S000000405DER1YBR201WverifiedEndoplasmic reticulum membrane protein, required forER-associated protein degradation of misfoldedor unassembled proteins; N- and C- terminiprotrude into the cytoplasm, has similarity toDfm1p 4e-050.00232 S000006375BSP1YPR171WverifiedAdapter that links synaptojanins Inp52p and Inp53p to thecortical actin cytoskeleton 4e-050.00232 S000005334BRE5YNR051CverifiedUbiquitin protease cofactor, forms deubiquitination complexwith Ubp3p that coregulates anterograde andretrograde transport between the endoplasmicreticulum and Golgi compartments; null issensitive to brefeldin A 4e-050.00232 S000004688ADH3YMR083WverifiedMitochondrial alcohol dehydrogenase isozyme III; involved inthe shuttling of mitochondrial NADH to thecytosol under anaerobic conditions and ethanolproduction 4e-050.00232 S000000182YBL086CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 4e-050.00232 S000005293CSE2YNR010WverifiedComponent of the Med9/10 module, which is a subcomplex withinthe RNA polymerase II Mediator complex;required for regulation of RNA polymerase IIactivity 4e-050.0023 S000005856SCD5YOR329CverifiedProtein required for normal cortical actin organization andendocytosis; multicopy suppressor of clathrindeficiency; acts as a targeting subunit forprotein phosphatase type 1 4e-050.0023 S000003033ALG2YGL065CverifiedPresumed early mannosyltransferase involved in the N-linkedglycosylation pathway; alg2 mutants exhibittemperature-sensitive growth and abnormalaccumulation of the lipid-linkedoligosaccharide Man2GlcNAc2-PP-Dol 4e-050.0023 S000004582TAF8YML114CverifiedTFIID subunit (65 kDa), involved in RNA polymerase IItranscription initiation 4e-050.0023 S000001128NAM8YHR086WverifiedRNA binding protein, component of the U1 snRNP protein; mutantsare defective in meiotic recombination and information of viable spores, involved in theformation of DSBs through meiosis-specificsplicing of MER2 pre-mRNA 4e-050.0023 S000004640MSN2YMR037CverifiedTranscriptional activator related to Msn4p; activated in stressconditions, which results in translocation fromthe cytoplasm to the nucleus; binds DNA atstress response elements of responsive genes,inducing gene expression 4e-050.0023 S000001059YSC83YHR017WverifiedNon-essential mitochondrial protein of unknown function; mRNAinduced during meiosis, peaking between mid tolate prophase of meiosis I; similar to S.douglasii YSD83 4e-050.0023 S000002570CWC15YDR163WverifiedNon-essential protein involved in pre-mRNA splicing, componentof a complex containing Cef1p; has similarityto S. pombe Cwf15p 4e-050.0023 S000006384AOS1YPR180WverifiedNuclear protein that acts as a heterodimer with Uba2p toactivate Smt3p (SUMO) before its conjugation toproteins (sumoylation), which may play a rolein protein targeting; essential for viability 4e-050.0023 S000004024SMF3YLR034CverifiedPutative divalent metal ion transporter involved in ironhomeostasis; transcriptionally regulated bymetal ions; member of the Nramp family of metaltransport proteins 4e-050.0023 S000005076KRE33YNL132WverifiedEssential protein of unknown function; heterozygous mutantshows haploinsufficiency in K1 killer toxinresistance 4e-050.0023 S000000141COR1YBL045CverifiedCore subunit of the ubiquinol-cytochrome c reductase complex(bc1 complex), which is a component of themitochondrial inner membrane electron transportchain 4e-050.0023 S000001393FKH1YIL131CverifiedForkhead family transcription factor with a minor role in theexpression of G2/M phase genes; negativelyregulates transcriptional elongation; positiverole in chromatin silencing at HML and HMR;regulates donor preference during switching 4e-050.0023 S000003618IML2YJL082WverifiedProtein of unknown function; the authentic, non-tagged proteinis detected in highly purified mitochondria inhigh-throughput studies 4e-050.0023 S000003819APS2YJR058CverifiedSmall subunit of the clathrin-associated adaptor complex AP-2,which is involved in protein sorting at theplasma membrane; related to the sigma subunitof the mammalian plasma membraneclathrin-associated protein (AP-2) complex 4e-050.0023 S000000741EDC3YEL015WverifiedNon-essential conserved protein of unknown function, plays arole in mRNA decapping by specificallyaffecting the function of the decapping enzymeDcp1p; localizes to cytoplasmic mRNA processingbodies 4e-050.0023 S000000339CKS1YBR135WverifiedSubunit of the Cdc28 protein kinase, required for mitoticproteolysis, may also be involved in theproteolysis of the G1 cyclins 4e-050.0023 S000002342YDL183CUncharacterizedPutative protein of unknown function; YBR063C is not an essential gene 4e-050.0023 S000006071YPL150WUncharacterizedHypothetical protein 4e-050.0023 S000005435YOL075CUncharacterizedHypothetical protein 4e-050.0023 S000028541YDR194W-AUncharacterizedPutative protein of unknown function; identified by fungal homology and RT-PCR 4e-050.0023 S000000503MAL32YBR299WverifiedMaltase (alpha-D-glucosidase), inducible protein involved inmaltose catabolism; encoded in the MAL3 complexlocus; functional in genomic reference strainS288C 4e-050.0023 S000006259SEC8YPR055WverifiedEssential 121kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 4e-050.0023 S000001241FMP22YHR198CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.0023 S000002612COQ4YDR204WverifiedProtein with a role in ubiquinone (Coenzyme Q) biosynthesis,possibly functioning in stabilization of Coq7p;located on the matrix face of the mitochondrialinner membrane; component of a mitochondrialubiquinone-synthesizing complex 4e-050.0023 S000005788YOR262WUncharacterizedProtein required for cell viability 4e-050.0023 S000004274ECI1YLR284CverifiedPeroxisomal delta3,delta2-enoyl-CoA isomerase, hexamericprotein that converts 3-hexenoyl-CoA totrans-2-hexenoyl-CoA, essential for thebeta-oxidation of unsaturated fatty acids,oleate-induced 4e-050.0023 S000003101ITC1YGL133WverifiedComponent of the ATP-dependent Isw2p-Itc1p chromatin remodelingcomplex, required for repression of a-specificgenes, repression of early meiotic genes duringmitotic growth, and repression of INO1 4e-050.0023 S000007620YLR363W-AUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus 4e-050.0023 S000006308FHL1YPR104CverifiedPutative transcriptional regulator with similarity toDNA-binding domain of Drosophila forkhead;required for rRNA processing 4e-050.0023 S000003091RPS2YGL123WverifiedProtein component of the small (40S) subunit, essential forcontrol of translational accuracy; hassimilarity to E. coli S5 and rat S2 ribosomalproteins 4e-050.0023 S000000016YAL018CUncharacterizedHypothetical protein 4e-050.00229 S000004238RCK2YLR248WverifiedProtein kinase involved in the response to oxidative andosmotic stress; identified as suppressor of S.pombe cell cycle checkpoint mutations 4e-050.00229 S000003159COX13YGL191WverifiedSubunit VIa of cytochrome c oxidase, which is the terminalmember of the mitochondrial inner membraneelectron transport chain; not essential forcytochrome c oxidase activity but may modulateactivity in response to ATP 4e-050.00229 S000003657RPE1YJL121CverifiedD-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in thenon-oxidative part of the pentose-phosphatepathway; mutants are sensitive to oxidativestress 4e-050.00229 S000001632DBR1YKL149CverifiedRNA lariat debranching enzyme, involved in intron turnover;required for efficient Ty1 transposition 4e-050.00229 S000002999RPL24AYGL031CverifiedRibosomal protein L30 of the large (60S) ribosomal subunit,nearly identical to Rpl24Bp and has similarityto rat L24 ribosomal protein; not essential fortranslation but may be required for normaltranslation rate 4e-050.00229 S000000120NCL1YBL024WverifiedS-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase,methylates cytosine to m5C at several positionsin tRNAs and intron-containing pre-tRNAs;similar to Nop2p and human proliferationassociated nucleolar protein p120 4e-050.00229 S000005553STI1YOR027WverifiedHsp90 cochaperone, interacts with the Ssa group of thecytosolic Hsp70 chaperones; activates theATPase activity of Ssa1p; homolog of mammalianHop protein 4e-050.00229 S000001156BZZ1YHR114WverifiedSH3 domain protein implicated in the regulation of actinpolymerization, able to recruit actinpolymerization machinery through its SH3domains, colocalizes with cortical actinpatches and Las17p, interacts with type Imyosins 4e-050.00229 S000001206SOL3YHR163Wverified6-phosphogluconolactonase, catalyzes the second step of thepentose phosphate pathway; weak multicopysuppressor of los1-1 mutation; homologous toSol2p and Sol1p 4e-050.00229 S000004957SPO1YNL012WverifiedMeiosis-specific protein with similarity to phospholipase B,required for meiotic spindle pole bodyduplication and separation; required for sporeformation 4e-050.00229 S000006323CLB2YPR119WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 4e-050.00229 S000003971YBT1YLL048CverifiedTransporter of the ATP-binding cassette (ABC) family involvedin bile acid transport; similar to mammalianbile transporters 4e-050.00229 S000000979BMH1YER177Wverified14-3-3 protein, major isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 4e-050.00229 S000006278TKL1YPR074CverifiedTransketolase, similar to Tkl2p; catalyzes conversion ofxylulose-5-phosphate and ribose-5-phosphate tosedoheptulose-7-phosphate andglyceraldehyde-3-phosphate in the pentosephosphate pathway; needed for synthesis ofaromatic amino acids 4e-050.00229 S000004165CBF5YLR175WverifiedPseudouridine synthase catalytic subunit of box H/ACA smallnucleolar ribonucleoprotein particles(snoRNPs), acts on both large and small rRNAsand on snRNA U2 4e-050.00229 S000003812OSM1YJR051WverifiedFumarate reductase, catalyzes the reduction of fumarate tosuccinate, required for the reoxidation ofintracellular NADH under anaerobic conditions;mutations cause osmotic sensitivity 4e-050.00229 S000006122YIG1YPL201CverifiedProtein that interacts with glycerol 3-phosphatase and plays arole in anaerobic glycerol production;localizes to the nucleus and cytosol 4e-050.00229 S000000305FES1YBR101CverifiedHsp70 (Ssa1p) nucleotide exchange factor, cytosolic homolog ofSil1p, which is the nucleotide exchange factorfor BiP (Kar2p) in the endoplasmic reticulum 4e-050.00229 S000001715MEH1YKR007WverifiedComponent of the EGO complex, which is involved in theregulation of microautophagy, and of the GSEcomplex, which is required for proper sortingof amino acid permease Gap1p; loss results in adefect in vacuolar acidification 4e-050.00229 S000004637YMR034CUncharacterizedPutative protein of unknown function with similarity to transporters; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene 4e-050.00229 S000001827YFL067WUncharacterizedHypothetical protein 4e-050.00229 S000002608VPS64YDR200CverifiedCytoplasmic protein required for cytoplasm to vacuole targetingof proteins; forms a complex with Far3p, Far7p,Far10p, and Far11p that is involved inpheromone-induced cell cycle arrest; alsolocalized to the endoplasmic reticulum membrane 4e-050.00229 S000000816HEM14YER014WverifiedProtoporphyrinogen oxidase, a mitochondrial enzyme thatcatalyzes the seventh step in the hemebiosynthetic pathway, convertingprotoporphyrinogen IX to protoporphyrin IX 4e-050.00229 S000004151YLR161WUncharacterizedPutative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames encoded near the ribosomal DNA region of chromosome 12 4e-050.00229 S000007274OLI1Q0130verifiedF0-ATP synthase subunit 9 (ATPase-associated proteolipid),encoded on the mitochondrial genome; mutationconfers oligomycin resistance; expression isspecifically dependent on the nuclear genesAEP1 and AEP2 4e-050.00229 S000006141RPL1AYPL220WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl1Bpand has similarity to E. coli L1 and rat L10aribosomal proteins; rpl1a rpl1b double nullmutation is lethal 4e-050.00229 S000003122LYS5YGL154CverifiedPhosphopantetheinyl transferase involved in lysinebiosynthesis; converts inactive apo-form ofLys2p (alpha-aminoadipate reductase) intocatalytically active holo-form byposttranslational addition ofphosphopantetheine 4e-050.00229 S000003016RPT6YGL048CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; bound byubiquitin-protein ligases Ubr1p and Ufd4p;localized mainly to the nucleus throughout thecell cycle 4e-050.00229 S000002998RPL30YGL030WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to rat L30 ribosomal protein;involved in pre-rRNA processing in thenucleolus; autoregulates splicing of itstranscript 4e-050.00229 S000002759SBE2YDR351WverifiedProtein involved in the transport of cell wall components fromthe Golgi to the cell surface; required for budgrowth 4e-050.00229 S000001661STE3YKL178CverifiedReceptor for a factor receptor, transcribed in alpha cells andrequired for mating by alpha cells, couples toMAP kinase cascade to mediate pheromoneresponse; ligand bound receptors areendocytosed and recycled to the plasmamembrane; GPCR 4e-050.00229 S000001731YKR023WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and mitochondrion 4e-050.00229 S000001264INP51YIL002CverifiedPhosphatidylinositol 4,5-bisphosphate 5-phosphatase,synaptojanin-like protein with an N-terminalSac1 domain, plays a role inphosphatidylinositol 4,5-bisphosphatehomeostasis and in endocytosis; null mutationconfers cold-tolerant growth 4e-050.00229 S000001667SPE1YKL184WverifiedOrnithine decarboxylase, catalyzes the first step in polyaminebiosynthesis; degraded in aproteasome-dependent manner in the presence ofexcess polyamines 4e-050.00229 S000003659YJL123CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); YJL123C is a non-essential gene 4e-050.00229 S000001025RPL8AYHL033CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Bp and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 4e-050.00229 S000003548RPC17YJL011CverifiedRNA polymerase III subunit C17; physically interacts with C31,C11, and TFIIIB70; may be involved in therecruitment of pol III by the preinitiationcomplex 4e-050.00229 S000003836HOC1YJR075WverifiedAlpha-1,6-mannosyltransferase involved in cell wall mannanbiosynthesis; subunit of a Golgi-localizedcomplex that also contains Anp1p, Mnn9p,Mnn11p, and Mnn10p; identified as a suppressorof a cell lysis sensitive pkc1-371 allele 4e-050.00229 S000003114YGL146CUncharacterizedHypothetical protein 4e-050.00229 S000004507RRN11YML043CverifiedProtein required for rDNA transcription by RNA polymerase I,component of the core factor (CF) of rDNAtranscription factor, which also contains Rrn6pand Rrn7p 4e-050.00229 S000001130RPF1YHR088WverifiedNucleolar protein involved in the assembly of the largeribosomal subunit; constituent of 66Spre-ribosomal particles; contains asigma(70)-like motif, which is thought to bindRNA 4e-050.00229 S000005103ASI2YNL159CverifiedIntegral inner nuclear membrane protein that acts with Asi1pand Asi3p to ensure the fidelity of SPS-sensorsignalling by maintaining the dormant repressedstate of gene expression in the absence ofinducing signals 4e-050.00229 S000004001LOT6YLR011WverifiedPutative NADPH-dependent FMN reductase with strong ferricyanidereductase activity in vitro; gene expressionincreases in cultures shifted to a lowertemperature 4e-050.00225 S000003950ISA1YLL027WverifiedMitochondrial matrix protein involved in biogenesis of theiron-sulfur (Fe/S) cluster of Fe/S proteins,isa1 deletion causes loss of mitochondrial DNAand respiratory deficiency; depletion reducesgrowth on nonfermentable carbon sources 4e-050.00225 S000004037FRE8YLR047CverifiedProtein with sequence similarity to iron/copper reductases,involved in iron homeostasis; deletion mutanthas iron deficiency/accumulation growthdefects; expression increased in the absence ofcopper-responsive transcription factor Mac1p 4e-050.00225 S000005207YIF1YNL263CverifiedIntegral membrane protein required for the fusion of ER-derivedCOPII transport vesicles with the Golgi;interacts with Yip1p and Yos1p; localizes tothe Golgi, the ER, and COPII vesicles 4e-050.00225 S000001882YFL012WUncharacterizedPutative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin 4e-050.00225 S000000812YER010CVerifiedProtein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction 4e-050.00225 S000004092APC9YLR102CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition 4e-050.00225 S000000034RBG1YAL036CverifiedMember of the DRG family of GTP-binding proteins; interactswith translating ribosomes and with Tma46p 4e-050.00225 S000028686YLR466C-BUncharacterizedDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data 4e-050.00225 S000002386HOYDL227CverifiedSite-specific endonuclease required for gene conversion at theMAT locus (homothallic switching) through thegeneration of a ds DNA break; expressionrestricted to mother cells in late G1 ascontrolled by Swi4p-Swi6p, Swi5p and Ash1p 4e-050.00225 S000004968FAP1YNL023CverifiedProtein that binds to Fpr1p (FKBP12), conferring rapamycinresistance by competing with rapamycin forFpr1p binding; has similarity to putativetranscription factors, including D.melanogaster shuttle craft and human NFX1 4e-050.00225 S000028437SLO1YER180C-AverifiedProtein interacting with Arl3p, which is a GTPase of the Rassuperfamily involved in vesicle-tethering atthe Golgi; putative ortholog of human SCOCO 4e-050.00225 S000028721YPL152W-AUncharacterizedIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 4e-050.00225 S000003357YGR125WUncharacterizedPutative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 4e-050.00225 S000003008HEM2YGL040CverifiedDelta-aminolevulinate dehydratase, a homo-octameric enzyme,catalyzes the conversion ofdelta-aminolevulinic acid to porphobilinogen,the second step in the heme biosyntheticpathway; localizes to both the cytoplasm andnucleus 4e-050.00225 S000004074RAX2YLR084CverifiedN-glycosylated protein involved in the maintenance of bud siteselection during bipolar budding; localizationrequires Rax1p 4e-050.00225 S000002663RMD5YDR255CverifiedCytosolic protein required for sporulation; also required forthe ubiquitination of the gluconeogeneticenzyme fructose-1,6-bisphosphatase, which isdegraded rapidly after the switch fromgluconeogenesis to glycolysis 4e-050.00225 S000004783HOT1YMR172WverifiedTranscription factor required for the transient induction ofglycerol biosynthetic genes GPD1 and GPP2 inresponse to high osmolarity; targets Hog1p toosmostress responsive promoters; has similarityto Msn1p and Gcr1p 4e-050.00225 S000007243MDM35YKL053C-AverifiedMitochondrial intermembrane space cysteine motif protein;mutation affects mitochondrial distribution andmorphology 4e-050.00225 S000002338PCL9YDL179WverifiedCyclin, forms a functional kinase complex with Pho85pcyclin-dependent kinase (Cdk), expressed inlate M/early G1 phase, activated by Swi5p 4e-050.00225 S000002979SCL1YGL011CverifiedAlpha subunit of the 20S core complex of the 26S proteasomeinvolved in the degradation of ubiquitinatedsubstrates; essential for growth; detected inthe mitochondria 4e-050.00225 S000003416UBR1YGR184CverifiedUbiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2pto ubiquitinate substrates of the N-end rulepathway; binds to the Rpn2p, Rpt1p, and Rpt6pproteins of the 19S particle of the 26Sproteasome 4e-050.00225 S000004121ACE2YLR131CverifiedTranscription factor that activates expression of earlyG1-specific genes, localizes to daughter cellnuclei after cytokinesis and delays G1progression in daughters, localization isregulated by phosphorylation; potential Cdc28psubstrate 4e-050.00225 S000005866UBC11YOR339CverifiedUbiquitin-conjugating enzyme most similar in sequence toXenopus ubiquitin-conjugating enzyme E2-C, butnot a true functional homolog of this E2;unlike E2-C, not required for the degradationof mitotic cyclin Clb2 4e-050.00225 S000001640APE2YKL157WverifiedZinc-dependent metallopeptidase yscII, may have a role inobtaining leucine from dipeptide substrates;sequence coordinates have changed since RT-PCRanalysis showed that the adjacent ORF YKL158Wcomprises the 5' exon of APE2/YKL157W 4e-050.00225 S000005348YSN1YNR065CuncharacterizedSortilin homolog, interacts with proteins of the endocyticmachinery 4e-050.00225 S000000804NOP16YER002WverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis 4e-050.00225 S000002861TSA2YDR453CverifiedStress inducible cytoplasmic thioredoxin peroxidase; cooperateswith Tsa1p in the removal of reactive oxygen,nitrogen and sulfur species using thioredoxinas hydrogen donor; deletion enhances themutator phenotype of tsa1 mutants 4e-050.00225 S000004547YML082WUncharacterizedPutative protein predicted to have carbon-sulfur lyase activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YNML082W is not an essential gene 4e-050.00225 S000004995YNL050CUncharacterizedPutative protein of unknown function; YNL050c is not an essential gene 4e-050.00225 S000005721SLK19YOR195WverifiedKinetochore-associated protein required for normal segregationof chromosomes in meiosis and mitosis;component of the FEAR regulatory network, whichpromotes Cdc14p release from the nucleolusduring anaphase; potential Cdc28p substrate 4e-050.00225 S000005549AHC1YOR023CverifiedSubunit of the Ada histone acetyltransferase complex, requiredfor structural integrity of the complex 4e-050.00225 S000006358PIN3YPR154WverifiedProtein that induces appearance of [PIN+] prion whenoverproduced 4e-050.00225 S000002335YDL176WUncharacterizedHypothetical protein 4e-050.00225 S000000699YCR102CUncharacterizedPutative protein of unknown function; involved in copper metabolism; similar to C.carbonum toxD gene; YCR102C is not an essential gene 4e-050.00225 S000002761TRR1YDR353WverifiedCytoplasmic thioredoxin reductase, key regulatory enzyme thatdetermines the redox state of the thioredoxinsystem, which acts as a disulfide reductasesystem and protects cells against bothoxidative and reductive stress 4e-050.00225 S000006209HAL1YPR005CverifiedCytoplasmic protein involved in halotolerance; decreasesintracellular Na+ (via Ena1p) and increasesintracellular K+ by decreasing efflux;expression repressed by Ssn6p-Tup1p and Sko1pand induced by NaCl, KCl, and sorbitol throughGcn4p 4e-050.00225 S000003260MSP1YGR028WverifiedMitochondrial protein involved in sorting of proteins in themitochondria; putative membrane-spanning ATPase 4e-050.00225 S000003189NIF3YGL221CverifiedProtein of unknown function, similar to Listeria monocytogenesmajor sigma factor (rpoD gene product); theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 4e-050.00225 S000005417GPM3YOL056WverifiedHomolog of Gpm1p phosphoglycerate mutase, which converts3-phosphoglycerate to 2-phosphoglycerate inglycolysis; may be non-functional derivative ofa gene duplication event 4e-050.00223 S000002980ERG4YGL012WverifiedC-24(28) sterol reductase, catalyzes the final step inergosterol biosynthesis; mutants are viable,but lack ergosterol 4e-050.00223 S000004873TIF11YMR260CverifiedTranslation initiation factor eIF1A, essential protein thatforms a complex with Sui1p (eIF1) and the 40Sribosomal subunit and scans for the startcodon; C-terminus associates with Fun12p(eIF5B); N terminus interacts with eIF2 andeIF3 4e-050.00223 S000006337SPN1YPR133CverifiedProtein that interacts with Spt6p and copurifies with Spt5p andRNA polymerase II, probable transcriptionalelongation factor; metazoan homologs contain anacidic N terminus; mutations in the gene conferan Spt- phenotype 4e-050.00223 S000002534ARO1YDR127WverifiedPentafunctional arom protein, catalyzes steps 2 through 6 inthe biosynthesis of chorismate, which is aprecursor to aromatic amino acids 4e-050.00223 S000004682VPS20YMR077CverifiedMyristoylated subunit of ESCRTIII, the endosomal sortingcomplex required for transport of transmembraneproteins into the multivesicular body pathwayto the lysosomal/vacuolar lumen; cytoplasmicprotein recruited to endosomal membranes 4e-050.00223 S000004603CDC5YMR001CverifiedPolo-like kinase with similarity to Xenopus Plx1 and S. pombePlo1p; found at bud neck, nucleus and SPBs; hasmultiple functions in mitosis and cytokinesisthrough phosphorylation of substrates; may be aCdc28p substrate 4e-050.00223 S000003319PDC6YGR087CverifiedMinor isoform of pyruvate decarboxylase, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde, regulation is glucose- andethanol-dependent, involved in amino acidcatabolism 4e-050.00223 S000003978YLL055WUncharacterizedPutative protein of unknown function with similarity to transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLL055W is not an essential gene 4e-050.00223 S000005873REV1YOR346WverifiedDeoxycytidyl transferase, forms a complex with the subunits ofDNA polymerase zeta, Rev3p and Rev7p; involvedin repair of abasic sites in damaged DNA 4e-050.00223 S000001366SHQ1YIL104CverifiedEssential nuclear protein, required for accumulation of boxH/ACA snoRNAs and for rRNA processing;interacts with Naf1p 4e-050.00223 S000002974PMC1YGL006WverifiedVacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ions; prevents growth inhibition by activationof calcineurin in the presence of elevatedconcentrations of calcium; similar to mammalianPMCA1a 4e-050.00223 S000028562YLR154C-HUncharacterizedPutative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-2 4e-050.00223 S000006105MRN1YPL184CuncharacterizedPutative RNA binding protein 4e-050.00223 S000003315GCD2YGR083CverifiedDelta subunit of the translation initiation factor eIF2B, theguanine-nucleotide exchange factor for eIF2;activity subsequently regulated byphosphorylated eIF2; first identified as anegative regulator of GCN4 expression 4e-050.00223 S000001784ECM4YKR076WverifiedOmega class glutathione transferase; not essential; similar toYgr154cp; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasm 4e-050.00223 S000005787RPN8YOR261CverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome;has similarity to the human p40 proteasomalsubunit and to another S. cerevisiae regulatorysubunit, Rpn11p 4e-050.00223 S000002799YDR391CUncharacterizedHypothetical protein 4e-050.00223 S000006074RAD53YPL153CverifiedProtein kinase, required for cell-cycle arrest in response toDNA damage; activated by transautophosphorylation when interacting withhyperphosphorylated Rad9p; also interacts withARS1 and plays a role in initiation of DNAreplication 4e-050.00223 S000000921AVT6YER119CverifiedVacuolar transporter, exports aspartate and glutamate from thevacuole; member of a family of seven S.cerevisiae genes (AVT1-7) related to vesicularGABA-glycine transporters 4e-050.00223 S000004551ALO1YML086CverifiedD-Arabinono-1,4-lactone oxidase, catalyzes the final step inbiosynthesis of D-erythroascorbic acid, whichis protective against oxidative stress 4e-050.00223 S000005707LAS17YOR181WverifiedActin assembly factor, activates the Arp2/3 protein complexthat nucleates branched actin filaments;localizes with the Arp2/3 complex to actinpatches; homolog of the human Wiskott-Aldrichsyndrome protein (WASP) 4e-050.00223 S000005790DSE3YOR264WverifiedDaughter cell-specific protein, may help establish daughterfate 4e-050.00223 S000003528YRF1-3YGR296WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 4e-050.00223 S000001228PFS1YHR185CverifiedSporulation protein required for prospore membrane formation atselected spindle poles, ensures functionalityof all four spindle pole bodies of a cellduring meiosis II; not required for meioticrecombination or meiotic chromosome segregation 4e-050.00223 S000001941YFR045WUncharacterizedPutative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white 4e-050.00223 S000005802CAF20YOR276WverifiedPhosphoprotein of the mRNA cap-binding complex involved intranslational control, repressor ofcap-dependent translation initiation, competeswith eIF4G for binding to eIF4E 4e-050.00223 S000004817INP1YMR204CverifiedPeripheral membrane protein of peroxisomes involved inperoxisomal inheritance 4e-050.00223 S000001359FYV10YIL097WverifiedProtein of unknown function, required for survival uponexposure to K1 killer toxin; involved inproteasome-dependent catabolite inactivation offructose-1,6-bisphosphatase; contains CTLHdomain 4e-050.00223 S000002419RPL4BYDR012WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl4Ap and has similarity to E.coli L4 and rat L4 ribosomal proteins 4e-050.00223 S000004147ASP3-2YLR157CverifiedCell-wall L-asparaginase II, involved in asparagine catabolism;expression is induced during nitrogenstarvation; four copies of ASP3 are present inthe genome reference strain S288C 4e-050.00223 S000002523MRPL1YDR116CverifiedMitochondrial ribosomal protein of the large subunit 4e-050.00223 S000001350AVT7YIL088CverifiedPutative transporter, member of a family of seven S. cerevisiaegenes (AVT1-7) related to vesicularGABA-glycine transporters 4e-050.00223 S000002995CWH41YGL027CverifiedProcessing alpha glucosidase I, ER type II integral membraneN-glycoprotein involved in assembly of cellwall beta 1,6 glucan and asparagine-linkedprotein glycosylation; also involved in ERprotein quality control and sensing of ERstress 4e-050.00223 S000003992NOC3YLR002CverifiedProtein that forms a nuclear complex with Noc2p that binds to66S ribosomal precursors to mediate theirintranuclear transport; also binds to chromatinto promote the association of DNA replicationfactors and replication initiation 4e-050.00223 S000006124TPK2YPL203WverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; activates filamentous growth 4e-050.00223 S000002408NTH1YDR001CverifiedNeutral trehalase, degrades trehalose; required forthermotolerance and may mediate resistance toother cellular stresses; may be phosphorylatedby Cdc28p 4e-050.00223 S000000730YEA4YEL004WverifiedUridine diphosphate-N-acetylglucosamine (UDP-GlcNAc)transporter required for cell wall chitinsynthesis; localized to the ER 4e-050.00223 S000003517ZUO1YGR285CverifiedCytosolic ribosome-associated chaperone that acts, togetherwith Ssz1p and the Ssb proteins, as a chaperonefor nascent polypeptide chains; contains a DnaJdomain and functions as a J-protein partner forSsb1p and Ssb2p 4e-050.00223 S000003379NAT2YGR147CverifiedN alpha-acetyl-transferase, transfers acetyl group from acetylcoenzyme A to the N-terminal methionineresidues of proteins 4e-050.00223 S000003991YLR001CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; also found in mitochondria; YLR001C is not an essential gene 4e-050.00223 S000000324CBP6YBR120CverifiedProtein required for translation of the mitochondrial COB mRNA 4e-050.00223 S000002476DOA4YDR069CverifiedUbiquitin hydrolase, required for recycling ubiquitin fromproteasome-bound ubiquitinated intermediates,acts at the late endosome/prevacuolarcompartment to recover ubiquitin fromubiquitinated membrane proteins en route to thevacuole 4e-050.00223 S000001324ARC15YIL062CverifiedSubunit of the ARP2/3 complex, which is required for themotility and integrity of cortical actinpatches 4e-050.00223 S000002539YDR132CUncharacterizedHypothetical protein 4e-050.00223 S000004303YLR312CUncharacterizedHypothetical protein 4e-050.00223 S000000756ECM10YEL030WverifiedHeat shock protein of the Hsp70 family, localized inmitochondrial nucleoids, plays a role inprotein translocation, interacts with Mge1p inan ATP-dependent manner; overexpression inducesextensive mitochondrial DNA aggregations 4e-050.00223 S000004639MIH1YMR036CverifiedProtein tyrosine phosphatase involved in cell cycle control;regulates the phosphorylation state of Cdc28p;homolog of S. pombe cdc25 4e-050.00223 S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 4e-050.00223 S000005752ISU2YOR226CverifiedConserved protein of the mitochondrial matrix, required forsynthesis of mitochondrial and cytosoliciron-sulfur proteins, performs a scaffoldingfunction in mitochondria during Fe/S clusterassembly; isu1 isu2 double mutant is inviable 4e-050.00223 S000000946UBP5YER144CverifiedPutative ubiquitin-specific protease, closest paralog of Doa4pbut has no functional overlap; concentrates atthe bud neck 4e-050.00223 S000113559YKL023C-AUncharacterizedPutative protein of unknown function 4e-050.00223 S000000009SWC3YAL011WverifiedProtein of unknown function, component of the SWR1 complex,which exchanges histone variant H2AZ (Htz1p)for chromatin-bound histone H2A; required forformation of nuclear-associated array of smoothendoplasmic reticulum known as karmellae 4e-050.00223 S000004699UTP15YMR093WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 4e-050.00223 S000005641TRS33YOR115CverifiedOne of 10 subunits of the transport protein particle (TRAPP)complex of the cis-Golgi which mediates vesicledocking and fusion; involved in endoplasmicreticulum (ER) to Golgi membrane traffic 4e-050.00223 S000003567MAD2YJL030WverifiedComponent of the spindle-assembly checkpoint complex, whichdelays the onset of anaphase in cells withdefects in mitotic spindle assembly; forms acomplex with Mad1p 4e-050.00223 S000000888ILV1YER086WverifiedThreonine deaminase, catalyzes the first step in isoleucinebiosynthesis; expression is under general aminoacid control; ILV1 locus exhibits highlypositioned nucleosomes whose organization isindependent of known ILV1 regulation 4e-050.00223 S000005338HOL1YNR055CverifiedPutative ion transporter similar to the major facilitatorsuperfamily of transporters; mutations inmembrane-spanning domains permit nonselectivecation uptake 4e-050.00223 S000004851DFG5YMR238WverifiedPutative mannosidase, essential glycosylphosphatidylinositol(GPI)-anchored membrane protein required forcell wall biogenesis in bud formation, involvedin filamentous growth, homologous to Dcw1p 4e-050.0022 S000001623TGL1YKL140WverifiedSteryl ester hydrolase, one of three gene products (Yeh1p,Yeh2p, Tgl1p) responsible for steryl esterhydrolase activity and involved in sterolhomeostasis; localized to lipid particlemembranes 4e-050.0022 S000004438YLR446WUncharacterizedPutative protein of unknown function with similarity to hexokinases; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene 4e-050.0022 S000006391RPO26YPR187WverifiedRNA polymerase subunit ABC23, common to RNA polymerases I, II,and III; part of central core; similar tobacterial omega subunit 4e-050.0022 S000001908YFR012WUncharacterizedHypothetical protein 4e-050.0022 S000006237HTS1YPR033CverifiedCytoplasmic and mitochondrial histidine tRNA synthetase;encoded by a single nuclear gene that specifiestwo messages; efficient mitochondriallocalization requires both a presequence and anamino-terminal sequence 4e-050.0022 S000001678MIA40YKL195WverifiedEssential protein of the mitochondrial intermembrane space(IMS); promotes retention of newly importedproteins; may do so by stabilizing clientprotein folding as part of a disulfide relaysystem or transferring metal to client proteins 4e-050.0022 S000001719YKR011CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus 4e-050.0022 S000001745SPC34YKR037CverifiedEssential subunit of the Dam1 complex (aka DASH complex),couples kinetochores to the force produced byMT depolymerization thereby aiding inchromosome segregation; also localized tonuclear side of spindle pole body 4e-050.0022 S000006051SPO19YPL130WverifiedMeiosis-specific protein of unknown function, involved incompletion of nuclear divisions; identified asa weak high-copy suppressor of the spo1-1 tsmutation; putative GPI-dependent cell-wallprotein 4e-050.0022 S000003112ROG1YGL144CverifiedProtein with putative serine active lipase domain 4e-050.0022 S000000075UIP3YAR027WverifiedPutative integral membrane protein of unknown function;interacts with Ulp1p at the nuclear periphery;member of DUP240 gene family 4e-050.0022 S000005097GIM3YNL153CverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 4e-050.0022 S000004308TAD3YLR316CverifiedSubunit of tRNA-specific adenosine-34 deaminase, forms aheterodimer with Tad2p that converts adenosineto inosine at the wobble position of severaltRNAs 4e-050.0022 S000028683YLR406C-AUncharacterizedPutative protein of unknown function 4e-050.0022 S000001247MNL1YHR204WverifiedAlpha mannosidase-like protein of the endoplasmic reticulumrequired for degradation of glycoproteins butnot for processing of N-linked oligosaccharides 4e-050.0022 S000006390PZF1YPR186CverifiedTranscription factor IIIA (TFIIIA), essential protein with nineC2H2 Zn-fingers, binds the 5S rRNA gene throughthe zinc finger domain and directs assembly ofa multiprotein initiation complex for RNApolymerase III; also binds DNA 4e-050.0022 S000004597MSC1YML128CverifiedProtein of unknown function; mutant is defective in directingmeiotic recombination events to homologouschromatids; the authentic, non-tagged proteinis detected in highly purified mitochondria inhigh-throughput studies 4e-050.0022 S000006312RPN7YPR108WverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome,similar to another S. cerevisiae regulatorysubunit, Rpn5p, as well as to mammalianproteasome subunits 4e-050.0022 S000005485TRM13YOL125WuncharacterizedHypothetical protein 4e-050.0022 S000004559GIM5YML094WverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 4e-050.0022 S000002535YDR128WUncharacterizedHypothetical protein 4e-050.0022 S000005504NOP8YOL144WverifiedNucleolar protein required for 60S ribosomal subunit biogenesis 4e-050.0022 S000001132YNG2YHR090CverifiedSubunit of the NuA4 histone acetyltransferase complex thatacetylates histone H4 and H2A; has similarityto the human tumor suppressor ING1 4e-050.0022 S000028828YHL015W-AUncharacterizedPutative protein of unknown function 4e-050.0022 S000003520MAL13YGR288WverifiedMAL-activator protein, part of complex locus MAL1;nonfunctional in genomic reference strain S288C 4e-050.0022 S000001569SRX1YKL086WverifiedSulfiredoxin, contributes to oxidative stress resistance byreducing cysteine-sulfinic acid groups in theperoxiredoxins Tsa1p and Ahp1p that are formedupon exposure to oxidants; conserved in highereukaryotes 4e-050.0022 S000005156VID27YNL212WverifiedCytoplasmic protein of unknown function; possibly involved invacuolar protein degradation; not essential forproteasome-dependent degradation offructose-1,6-bisphosphatase (FBPase); nullmutants exhibit normal growth 4e-050.0022 S000005544ROD1YOR018WverifiedMembrane protein; overexpression confers resistance to the GSTsubstrate o-dinitrobenzene as well as to zincand calcium; contains 2 PY motifs, which arerequired for Rod1p interaction with Rsp5p, ahect-type ubiquitin ligase 4e-050.0022 S000003557BBC1YJL020CverifiedProtein possibly involved in assembly of actin patches;interacts with an actin assembly factor Las17pand with the SH3 domains of Type I myosinsMyo3p and Myo5p; localized predominantly tocortical actin patches 4e-050.0022 S000006306RPL11AYPR102CverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl11Bp; involved in ribosomalassembly; depletion causes degradation ofproteins and RNA of the 60S subunit; hassimilarity to E. coli L5 and rat L11 4e-050.0022 S000004621STB4YMR019WverifiedProtein that binds Sin3p in a two-hybrid assay; contains aZn(II)2Cys6 zinc finger domain characteristicof DNA-binding proteins 4e-050.0022 S000003071RPL28YGL103WverifiedRibosomal protein L29 of the large (60S) ribosomal subunit, hassimilarity to E. coli L15 and rat L27aribosomal proteins; may have peptidyltransferase activity; can mutate tocycloheximide resistance 4e-050.0022 S000005678YOR152CUncharacterizedHypothetical protein 4e-050.0022 S000006266FCY1YPR062WverifiedCytosine deaminase, zinc metalloenzyme that catalyzes thehydrolytic deamination of cytosine to uracil;of biomedical interest because it alsocatalyzes the deamination of 5-fluorocytosine(5FC) to form anticancer drug 5-fluorouracil(5FU) 4e-050.0022 S000003856SFC1YJR095WverifiedMitochondrial succinate-fumarate transporter, transportssuccinate into and fumarate out of themitochondrion; required for ethanol and acetateutilization 4e-050.0022 S000005509DCP1YOL149WverifiedSubunit of the Dcp1p-Dcp2p decapping enzyme complex, whichremoves the 5' cap structure from mRNAs priorto their degradation; enhances the activity ofcatalytic subunit Dcp2p; regulated by DEAD boxprotein Dhh1p 4e-050.0022 S000003078CUE3YGL110CuncharacterizedProtein of unknown function; has a CUE domain that bindsubiquitin, which may facilitate intramolecularmonoubiquitination 4e-050.0022 S000003786BNA1YJR025Cverified3-hydroxyanthranilic acid dioxygenase, required forbiosynthesis of nicotinic acid from tryptophanvia kynurenine pathway 4e-050.0022 S000004240SSP120YLR250WverifiedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmin a punctate pattern 4e-050.0022 S000006437RPL29YFR032C-AverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to rat L29 ribosomal protein; notessential for translation, but required forproper joining of the large and small ribosomalsubunits and for normal translation rate 4e-050.0022 S000006195SAM3YPL274WverifiedHigh-affinity S-adenosylmethionine permease, required forutilization of S-adenosylmethionine as a sulfursource; has similarity to S-methylmethioninepermease Mmp1p 4e-050.0022 S000004620YMR018WUncharacterizedPutative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene 4e-050.0022 S000002196YDL038CUncharacterizedPutative protein of unknown function; YDL038C is not an essential gene 4e-050.0022 S000003513YOR1YGR281WverifiedPlasma membrane transporter of the ATP-binding cassette (ABC)family, mediates export of many differentorganic anions including oligomycin 4e-050.0022 S000001153UBA4YHR111WverifiedProtein that activates Urm1p before its conjugation to proteins(urmylation); one target is the thioredoxinperoxidase Ahp1p, suggesting a role ofurmylation in the oxidative stress response 4e-050.0022 S000003775TMA22YJR014WverifiedProtein of unknown function that associates with ribosomes andhas a putative RNA binding domain; interactswith Tma20p; has homology to human proteinDRP1, which interacts with human Tma20p homologMCT-1 4e-050.0022 S000003843ACF4YJR083CuncharacterizedProtein of unknown function, computational analysis oflarge-scale protein-protein interaction datasuggests a possible role in actin cytoskeletonorganization; potential Cdc28p substrate 4e-050.0022 S000002678CCC2YDR270WverifiedCu(+2)-transporting P-type ATPase, required for export ofcopper from the cytosol into an extracytosoliccompartment; has similarity to human proteinsinvolved in Menkes and Wilsons diseases 4e-050.0022 S000004672AVO2YMR068WverifiedComponent of a complex containing the Tor2p kinase and otherproteins, which may have a role in regulationof cell growth 4e-050.0022 S000003343YGR111WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 4e-050.0022 S000005518ENB1YOL158CverifiedEndosomal ferric enterobactin transporter, expressed underconditions of iron deprivation; member of themajor facilitator superfamily; expression isregulated by Rcs1p and affected by chloroquinetreatment 4e-050.0022 S000002462PST1YDR055WverifiedCell wall protein that contains a putative GPI-attachment site;secreted by regenerating protoplasts;up-regulated by activation of the cellintegrity pathway, as mediated by Rlm1p;upregulated by cell wall damage via disruptionof FKS1 4e-050.0022 S000002459DBF4YDR052CverifiedRegulatory subunit of Cdc7p-Dbf4p kinase complex, required forCdc7p kinase activity and initiation of DNAreplication; phosphorylates the Mcm2-7 familyof proteins; cell cycle regulated 4e-050.0022 S000000965YER163CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 4e-050.0022 S000007279SCEIQ0160verifiedI-SceI DNA endonuclease, encoded by the mitochondrial group Iintron of the 21S_rRNA gene; mediates geneconversion that propagates the intron intointron-less copies of the 21S_rRNA gene 4e-050.0022 S000001707PAU16YKL224CuncharacterizedPutative protein of unknown function 4e-050.0022 S000004202TUB4YLR212CverifiedGamma-tubulin, involved in nucleating microtubules from boththe cytoplasmic and nuclear faces of thespindle pole body 4e-050.0022 S000000844MXR1YER042WverifiedPeptide methionine sulfoxide reductase, reverses the oxidationof methionine residues; involved in oxidativedamage repair, providing resistance tooxidative stress and regulation of lifespan 4e-050.0022 S000000198SFT2YBL102WverifiedNon-essential tetra-spanning membrane protein found mostly inthe late Golgi, can suppress some sed5 alleles;may be part of the transport machinery, butprecise function is unknown; similar tomammalian syntaxin 5 4e-050.0022 S000004652ERB1YMR049CverifiedProtein required for maturation of the 25S and 5.8S ribosomalRNAs; constituent of 66S pre-ribosomalparticles; homologous to mammalian Bop1 4e-050.0022 S000004534POB3YML069WverifiedSubunit of the heterodimeric FACT complex (Spt16p-Pob3p), whichfacilitates RNA Polymerase II transcriptionelongation through nucleosomes by destabilizingand then reassembling nucleosome structure 4e-050.0022 S000003000AGA2YGL032CverifiedAdhesion subunit of a-agglutinin of a-cells, C-terminalsequence acts as a ligand for alpha-agglutinin(Sag1p) during agglutination, modified withO-linked oligomannosyl chains, linked toanchorage subunit Aga1p via two disulfide bonds 4e-050.0022 S000000532FUS1YCL027WverifiedMembrane protein localized to the shmoo tip, required for cellfusion; expression regulated by matingpheromone; proposed to coordinate signaling,fusion, and polarization events required forfusion; potential Cdc28p substrate 4e-050.0022 S000001543FBA1YKL060CverifiedFructose 1,6-bisphosphate aldolase, required for glycolysis andgluconeogenesis; catalyzes conversion offructose 1,6 bisphosphate to glyceraldehyde-3-Pand dihydroxyacetone-P; locates tomitochondrial outer surface upon oxidativestress 4e-050.0022&radic S000007265AI5_ALPHAQ0070verifiedEndonuclease I-SceIV, involved in intron mobility; encoded by amobile group I intron within the mitochondrialCOX1 gene 4e-050.0022 S000002954SOM1YEL059C-AverifiedSubunit of the mitochondrial inner membrane peptidase, which isrequired for maturation of mitochondrialproteins of the intermembrane space; Som1pfacilitates cleavage of a subset of substrates 4e-050.0022 S000001379PRM5YIL117CverifiedPheromone-regulated protein, predicted to have 1 transmembranesegment; induced during cell integritysignaling 4e-050.0022 S000003754YJL218WUncharacterizedPutative protein of unknown function, similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene 4e-050.0022 S000005394SMC5YOL034WverifiedStructural maintenance of chromosomes (SMC) protein; essentialsubunit of the Mms21-Smc5-Smc6 complex;required for growth and DNA repair; S. pombehomolog forms a heterodimer with S. pombeRad18p that is involved in DNA repair 4e-050.0022 S000006093COX10YPL172CverifiedHeme A:farnesyltransferase, catalyzes the first step in theconversion of protoheme to the heme Aprosthetic group required for cytochrome coxidase activity; human ortholog is associatedwith mitochondrial disorders 4e-050.0022 S000000035YAL037WUncharacterizedHypothetical protein 4e-050.0022 S000002547MTQ2YDR140WuncharacterizedS-adenosylmethionine-dependent methyltransferase of the sevenbeta-strand family; methylates release factoreRF1 (Sup45p) in vitro; is not an essentialgene; similar to E.coli PrmC 4e-050.0022 S000002438MIC14YDR031WverifiedMitochondrial intermembrane space cysteine motif protein of 14kDa 4e-050.0022 S000004951LST8YNL006WverifiedProtein required for the transport of amino acid permease Gap1pfrom the Golgi to the cell surface; componentof the TOR signaling pathway; associates withboth Tor1p and Tor2p; contains a WD-repeat 4e-050.0022 S000000766UTR2YEL040WverifiedPutative glycosidase, glycosylphosphatidylinositol(GPI)-anchored protein localized to the budneck; has a role in cell wall maintenance 4e-050.0022 S000004144RNH203YLR154CverifiedRibonuclease H2 subunit, required for RNase H2 activity 4e-050.0022 S000004690YMR085WUncharacterizedHypothetical protein 4e-050.0022 S000005971MNN9YPL050CverifiedSubunit of Golgi mannosyltransferase complex also containingAnp1p, Mnn10p, Mnn11p, and Hoc1p that mediateselongation of the polysaccharide mannanbackbone; forms a separate complex with Van1pthat is also involved in backbone elongation 4e-050.0022 S000004313SFH1YLR321CverifiedSubunit of the RSC chromatin remodeling complex required forkinetochore function in chromosome segregation;essential gene required for cell cycleprogression; phosphorylated in the G1 phase ofthe cell cycle; Snf5p paralog 4e-050.0022 S000000354TBS1YBR150CverifiedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.0022 S000004064BUD20YLR074CverifiedProtein involved in bud-site selection; diploid mutants displaya random budding pattern instead of thewild-type bipolar pattern 4e-050.0022 S000004361SSQ1YLR369WverifiedMitochondrial hsp70-type molecular chaperone, required forassembly of iron/sulfur clusters into proteinsat a step after cluster synthesis, and formaturation of Yfh1p, which is a homolog ofhuman frataxin implicated in Friedreich'sataxia 4e-050.0022 S000006045SPC29YPL124WverifiedInner plaque spindle pole body (SPB) component, links thecentral plaque component Spc42p to the innerplaque component Spc110p; required for SPBduplication 4e-050.0022 S000004138PEP3YLR148WverifiedVacuolar peripheral membrane protein that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, required for vacuolarbiogenesis, forms complex with Pep5p thatmediates protein transport to the vacuole 4e-050.0022 S000003838MIR1YJR077CverifiedMitochondrial phosphate carrier, imports inorganic phosphateinto mitochondria; functionally redundant withPic2p but more abundant than Pic2 under normalconditions 4e-050.0022 S000000347SUP45YBR143CverifiedPolypeptide release factor involved in translation termination;mutant form acts as a recessive omnipotentsuppressor 4e-050.0022 S000006210ICL2YPR006Cverified2-methylisocitrate lyase of the mitochondrial matrix, functionsin the methylcitrate cycle to catalyze theconversion of 2-methylisocitrate to succinateand pyruvate; ICL2 transcription is repressedby glucose and induced by ethanol 4e-050.0022 S000005016RNH201YNL072WverifiedRibonuclease H2 catalytic subunit, removes RNA primers duringOkazaki fragment synthesis; cooperates withRad27p nuclease 4e-050.0022 S000000429YBR225WUncharacterizedHypothetical protein 4e-050.0022 S000001053DIA4YHR011WverifiedProbable mitochondrial seryl-tRNA synthetase, mutant displaysincreased invasive and pseudohyphal growth 4e-050.0022 S000005902GDH1YOR375CverifiedNADP(+)-dependent glutamate dehydrogenase, synthesizesglutamate from ammonia and alpha-ketoglutarate;rate of alpha-ketoglutarate utilization differsfrom Gdh3p; expression regulated by nitrogenand carbon sources 4e-050.0022 S000005342MNT4YNR059WverifiedPutative alpha-1,3-mannosyltransferase, not required forprotein O-glycosylation 4e-050.0022 S000000818BIM1YER016WverifiedMicrotubule-binding protein that together with Kar9p makes upthe cortical microtubule capture site anddelays the exit from mitosis when the spindleis oriented abnormally 4e-050.0022 S000002672AKR1YDR264CverifiedPalmitoyl transferase involved in protein palmitoylation; actsas a negative regulator of pheromone responsepathway; required for endocytosis of pheromonereceptors; involved in cell shape control;contains ankyrin repeats 4e-050.0022 S000006078TGS1YPL157WverifiedTrimethyl guanosine synthase, conserved nucleolar methyltransferase responsible for conversion of them(7)G cap structure of snRNAs and snoRNAs tom(2,2,7)G; also required for ribosome synthesisand nucleolar morphology 4e-050.0022 S000005554CIN5YOR028CverifiedBasic leucine zipper transcriptional factor of the yAP-1 familythat mediates pleiotropic drug resistance andsalt tolerance; localizes constitutively to thenucleus 4e-050.0022 S000005337ESF2YNR054CverifiedEssential nucleolar protein involved in pre-18S rRNAprocessing; component of the small subunit(SSU) processome; has sequence similarity tomABT1, a mouse transcription activator 4e-050.0022 S000004615SEC59YMR013CverifiedDolichol kinase, catalyzes the terminal step in dolichylmonophosphate (Dol-P) biosynthesis; requiredfor viability and for normal rates of lipidintermediate synthesis and proteinN-glycosylation 4e-050.0022 S000002923SLF1YDR515WverifiedRNA binding protein that associates with polysomes; proposed tobe involved in regulating mRNA translation;involved in the copper-dependent mineralizationof copper sulfide complexes on cell surface incells cultured in copper salts 4e-050.0022 S000001852YFL042CUncharacterizedPutative protein of unknown function; YFL042C is not an essential gene 4e-050.0022 S000004965ARK1YNL020CverifiedSerine/threonine protein kinase involved in regulation of thecortical actin cytoskeleton; involved incontrol of endocytosis 4e-050.0022 S000001627RPC25YKL144CverifiedRNA polymerase III subunit C25 4e-050.0022 S000002706ATP5YDR298CverifiedSubunit 5 of the stator stalk of mitochondrial F1F0 ATPsynthase, which is a large, evolutionarilyconserved enzyme complex required for ATPsynthesis; homologous to bovine subunit OSCP(oligomycin sensitivity-conferring protein) 4e-050.0022 S000004841MTF1YMR228WverifiedMitochondrial RNA polymerase specificity factor with structuralsimilarity to S-adenosylmethionine-dependentmethyltransferases and functional similarity tobacterial sigma-factors, interacts withmitochondrial core polymerase Rpo41p 4e-050.0022 S000000399MSI1YBR195CverifiedSubunit of chromatin assembly factor I (CAF-I), regulates theRAS/cAMP pathway via sequestration of Npr1pkinase; localizes to the nucleus and cytoplasm;homologous to human retinoblastoma bindingproteins RbAp48 and RbAp46 4e-050.0022 S000000358RPB5YBR154CverifiedRNA polymerase subunit ABC27, common to RNA polymerases I, II,and III; contacts DNA and affectstransactivation 4e-050.0022 S000004137SMD3YLR147CverifiedCore Sm protein Sm D3; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D3 4e-050.0022&radic S000003608PSF2YJL072CverifiedSubunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), whichis localized to DNA replication origins andimplicated in assembly of the DNA replicationmachinery 4e-050.0022 S000005588YOR062CUncharacterizedProtein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p 4e-050.0022 S000003197SHE10YGL228WverifiedPutative glycosylphosphatidylinositol (GPI)-anchored protein ofunknown function; overexpression causes growtharrest 4e-050.0022 S000004012SDO1YLR022CuncharacterizedEssential protein involved in RNA metabolism, one of two yeasthomologs (with Yhr087wp) of the human proteinSBDS responsible for autosomal recessiveShwachman-Bodian-Diamond Syndrome, alsoconserved in Archaea 4e-050.0022 S000004822YMR209CUncharacterizedPutative protein of unknown function; YMR209C is not an essential gene 4e-050.0022 S000003771MET3YJR010WverifiedATP sulfurylase, catalyzes the primary step of intracellularsulfate activation, essential for assimilatoryreduction of sulfate to sulfide, involved inmethionine metabolism 4e-050.0022 S000006153SSO1YPL232WverifiedPlasma membrane t-SNARE involved in fusion of secretoryvesicles at the plasma membrane and in vesiclefusion during sporulation; forms a complex withSec9p that binds v-SNARE Snc2p; syntaxinhomolog; functionally redundant with Sso2p 4e-050.0022 S000005784HNT3YOR258WverifiedMember of the third branch of the histidine triad (HIT)superfamily of nucleotide-binding proteins;similar to Aprataxin, a Hint related proteinthat is mutated in individuals with ataxia withoculomotor apraxia 4e-050.0022 S000003722MNN5YJL186WverifiedAlpha-1,2-mannosyltransferase, responsible for addition of thesecond alpha-1,2-linked mannose of the brancheson the mannan backbone of oligosaccharides,localizes to an early Golgi compartment 4e-050.0022 S000004714ILV2YMR108WverifiedAcetolactate synthase, catalyses the first common step inisoleucine and valine biosynthesis and is thetarget of several classes of inhibitors,localizes to the mitochondria; expression ofthe gene is under general amino acid control 4e-050.0022 S000006227EAF3YPR023CverifiedEsa1p-associated factor, nonessential component of the NuA4acetyltransferase complex, homologous toDrosophila dosage compensation protein MSL3 4e-050.0022 S000001332MAM33YIL070CverifiedAcidic protein of the mitochondrial matrix involved inoxidative phosphorylation; related to the humancomplement receptor gC1q-R 4e-050.0022 S000001492MRT4YKL009WverifiedProtein involved in mRNA turnover and ribosome assembly,localizes to the nucleolus 4e-050.0022 S000002333DLD1YDL174CverifiedD-lactate dehydrogenase, oxidizes D-lactate to pyruvate,transcription is heme-dependent, repressed byglucose, and derepressed in ethanol or lactate;located in the mitochondrial inner membrane 4e-050.0022 S000028571YLR342W-AUncharacterizedPutative protein of unknown function 4e-050.0022 S000002732UTP4YDR324CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 4e-050.0022 S000005848PMT3YOR321WverifiedProtein O-mannosyltransferase, transfers mannose residues fromdolichyl phosphate-D-mannose to proteinserine/threonine residues; acts in a complexwith Pmt5p, can instead interact with Pmt1p insome conditions; target for new antifungals 4e-050.0022 S000003750HXT8YJL214WverifiedProtein of unknown function with similarity to hexosetransporter family members, expression isinduced by low levels of glucose and repressedby high levels of glucose 4e-050.0022 S000004196ENT2YLR206WverifiedEpsin-like protein required for endocytosis and actin patchassembly and functionally redundant with Ent1p;contains clathrin-binding motif at C-terminus 4e-050.0022 S000005190VPS75YNL246WverifiedProtein of unknown function involved in vacuolar proteinsorting; detected in the nucleus 4e-050.0022 S000001390MET18YIL128WverifiedDNA repair and TFIIH regulator, required for both nucleotideexcision repair (NER) and RNA polymerase II(RNAP II) transcription; involved in telomeremaintenance 4e-050.0022 S000002390BRE4YDL231CverifiedZinc finger protein containing five transmembrane domains; nullmutant exhibits strongly fragmented vacuolesand sensitivity to brefeldin A, a drug which isknown to affect intracellular transport 4e-050.0022 S000000404BEM1YBR200WverifiedProtein containing SH3-domains, involved in establishing cellpolarity and morphogenesis; functions as ascaffold protein for complexes that includeCdc24p, Ste5p, Ste20p, and Rsr1p 4e-050.0022 S000006199YPL278CUncharacterizedHypothetical protein 4e-050.0022 S000005211PIK1YNL267WverifiedPhosphatidylinositol 4-kinase; catalyzes first step in thebiosynthesis ofphosphatidylinositol-4,5-biphosphate; maycontrol cytokineses through the actincytoskeleton 4e-050.0022 S000001082BCD1YHR040WverifiedEssential protein required for the accumulation of box C/DsnoRNA 4e-050.0022 S000000361ICS2YBR157CverifiedProtein of unknown function; null mutation does not confer anyobvious defects in growth, spore germination,viability, or carbohydrate utilization 4e-050.0022 S000003571KAR2YJL034WverifiedATPase involved in protein import into the ER, also acts as achaperone to mediate protein folding in the ERand may play a role in ER export of solubleproteins; regulates the unfolded proteinresponse via interaction with Ire1p 4e-050.0022 S000004230VPS34YLR240WverifiedPhosphatidylinositol 3-kinase responsible for the synthesis ofphosphatidylinositol 3-phosphate; formsmembrane-associated signal transduction complexwith Vps15p to regulate protein sorting;activated by the GTP-bound form of Gpa1p 4e-050.0022 S000003757FSP2YJL221CverifiedProtein of unknown function, expression is induced duringnitrogen limitation 4e-050.0022 S000002793EFT2YDR385WverifiedElongation factor 2 (EF-2), also encoded by EFT1; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 4e-050.0022 S000001737SET3YKR029CverifiedDefining member of the SET3 histone deacetylase complex whichis a meiosis-specific repressor of sporulationgenes; necessary for efficient transcription byRNAPII; one of two yeast proteins that containsboth SET and PHD domains 4e-050.0022 S000028804YJL047C-AUncharacterized 4e-050.0022 S000003768YJR008WUncharacterizedPutative protein of unknown function; expression repressed by inosine and choline in an Opi1p-dependent manner; expression induced by mild heat-stress on a non-fermentable carbon source. 4e-050.0022 S000002100VMA10YHR039C-AverifiedVacuolar H+ ATPase subunit G of the catalytic (V1) sector,involved in vacuolar acidification 4e-050.0022 S000004468MRPL39YML009CverifiedMitochondrial ribosomal protein of the large subunit 4e-050.0022 S000003945HIF1YLL022CverifiedNon-essential component of the HAT-B histone acetyltransferasecomplex (Hat1p-Hat2p-Hif1p), localized to thenucleus; has a role in telomeric silencing 4e-050.0022 S000004704YMR098CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR098C is not an essential gene 4e-050.0022 S000002398ADY3YDL239CverifiedProtein required for spore wall formation, thought to mediateassembly of a Don1p-containing structure at theleading edge of the prospore membrane viainteraction with spindle pole body components;potentially phosphorylated by Cdc28p 4e-050.0022 S000000738UBC8YEL012WverifiedUbiquitin-conjugating enzyme that negatively regulatesgluconeogenesis by mediating theglucose-induced ubiquitination offructose-1,6-bisphosphatase (FBPase);cytoplasmic enzyme that catalyzes theubiquitination of histones in vitro 4e-050.0022 S000003540COX16YJL003WverifiedMitochondrial inner membrane protein, required for assembly ofcytochrome c oxidase 4e-050.0022&radic S000006261BRR1YPR057WverifiedsnRNP protein component of spliceosomal snRNPs, required forpre-mRNA splicing and snRNP biogenesis; in nullmutant newly-synthesized snRNAs aredestabilized and 3'-end processing is slowed 4e-050.0022&radic S000007228SCS22YBL091C-AverifiedProtein involved in regulation of phospholipid metabolism;homolog of Scs2p2; similar to D. melanogasterinturned protein 4e-050.0022 S000006024FMP30YPL103CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.0022 S000004893CAT8YMR280CverifiedZinc cluster transcriptional activator necessary forderepression of a variety of genes undernon-fermentative growth conditions, activeafter diauxic shift, binds carbon sourceresponsive elements 4e-050.0022 S000002688RRP45YDR280WverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 4e-050.0022 S000004701SNO1YMR095CverifiedProtein of unconfirmed function, involved in pyridoxinemetabolism; expression is induced duringstationary phase; forms a putative glutamineamidotransferase complex with Snz1p, with Sno1pserving as the glutaminase 4e-050.0022 S000001380RHO3YIL118WverifiedNon-essential small GTPase of the Rho/Rac subfamily of Ras-likeproteins involved in the establishment of cellpolarity; GTPase activity positively regulatedby the GTPase activating protein (GAP) Rgd1p 4e-050.00216&radic S000006100PPQ1YPL179WverifiedPutative protein serine/threonine phosphatase; null mutationenhances efficiency of translationalsuppressors 4e-050.00216 S000003294COX18YGR062CverifiedMitochondrial inner membrane protein, required for export ofthe Cox2p C terminus from the mitochondrialmatrix to the intermembrane space during itsassembly into cytochrome c oxidase; similar toOxa2p of N. crassa 4e-050.00216 S000000230ETR1YBR026Cverified2-enoyl thioester reductase, member of the medium chaindehydrogenase/reductase family; localized to inmitochondria, where it has a probable role infatty acid synthesis 4e-050.00216 S000001498PUT3YKL015WverifiedTranscriptional activator of proline utilization genes,constitutively binds PUT1 and PUT2 promotersequences and undergoes a conformational changeto form the active state; has a Zn(2)-Cys(6)binuclear cluster domain 4e-050.00216 S000003279TFC4YGR047CverifiedOne of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauA domain of TFIIIC that binds BoxA DNApromoter sites of tRNA and similar genes; hasTPR motifs; human homolog is TFIIIC-102 4e-050.00216 S000005313ALG12YNR030WverifiedAlpha-1,6-mannosyltransferase localized to the ER; responsiblefor the addition of the alpha-1,6 mannose todolichol-linked Man7GlcNAc2, acts in thedolichol pathway for N-glycosylation 4e-050.00216 S000003670LCB3YJL134WverifiedLong-chain base-1-phosphate phosphatase, regulates ceramide andlong-chain base phosphates levels, involved inincorporation of exogenous long chain bases insphingolipids 4e-050.00216 S000005992YPL071CUncharacterizedHypothetical protein 4e-050.00216 S000000949SCC4YER147CverifiedSubunit of cohesin loading factor (Scc2p-Scc4p), a complexrequired for the loading of cohesin complexesonto chromosomes; involved in establishingsister chromatid cohesion during double-strandbreak repair via phosphorylated histone H2AX 4e-050.00216 S000002485SHU2YDR078CuncharacterizedProtein of unassigned function involved in mutationsuppression, important for error-free repair ofspontaneous and induced DNA lesions to protectthe genome from mutation; associates withShu1p, Psy3p, and Csm2p 4e-050.00216 S000006255MAK3YPR051WverifiedCatalytic subunit of N-terminal acetyltransferase of the NatCtype; required for replication of dsRNA virus 4e-050.00216 S000003139ROK1YGL171WverifiedATP-dependent RNA helicase of the DEAD box family; required for18S rRNA synthesis 4e-050.00216 S000002385GCS1YDL226CverifiedADP-ribosylation factor GTPase activating protein (ARF GAP),involved in ER-Golgi transport; sharesfunctional similarity with Glo3p 4e-050.00216 S000006362YPR158WUncharacterizedHypothetical protein 4e-050.00216 S000002363RTN2YDL204WverifiedProtein of unknown function; has similarity to mammalianreticulon proteins; member of the RTNLA(reticulon-like A) subfamily 4e-050.00216 S000002856ADA2YDR448WverifiedTranscription coactivator, component of the ADA and SAGAtranscriptional adaptor/HAT (histoneacetyltransferase) complexes 4e-050.00216 S000000479RIF1YBR275CverifiedProtein that binds to the Rap1p C-terminus and actssynergistically with Rif2p to help controltelomere length and establish telomericsilencing; deletion results in telomereelongation 4e-050.00216 S000001170FUR1YHR128WverifiedUracil phosphoribosyltransferase, synthesizes UMP from uracil;involved in the pyrimidine salvage pathway 4e-050.00216 S000001718TOF2YKR010CverifiedNonessential mitochondrial protein of unknown function withsequence similarity to Net1p; identified as atopoisomerase I (Top1p) binding protein;displays synthetic genetic interactions withTOP1 and HPR1 4e-050.00216 S000003909BAT2YJR148WverifiedCytosolic branched-chain amino acid aminotransferase, homologof murine ECA39; highly expressed duringstationary phase and repressed duringlogarithmic phase 4e-050.00216 S000003162HOS2YGL194CverifiedHistone deacetylase required for gene activation via specificdeacetylation of lysines in H3 and H4 histonetails; subunit of the Set3 complex, ameiotic-specific repressor of sporulationspecific genes that contains deacetylaseactivity 4e-050.00216 S000004663SEN15YMR059WverifiedSubunit of the tRNA splicing endonuclease, which is composed ofSen2p, Sen15p, Sen34p, and Sen54p 4e-050.00216 S000004696YMR090WUncharacterizedPutative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR090W is not an essential gene 4e-050.00216 S000003996SSK1YLR006CverifiedCytoplasmic response regulator, part of a two-component signaltransducer that mediates osmosensing via aphosphorelay mechanism; dephosphorylated formis degraded by the ubiquitin-proteasome system;potential Cdc28p substrate 4e-050.00216 S000003224ZRT1YGL255WverifiedHigh-affinity zinc transporter of the plasma membrane,responsible for the majority of zinc uptake;transcription is induced under low-zincconditions by the Zap1p transcription factor 4e-050.00216 S000001335SPO22YIL073CverifiedMeiosis-specific protein essential for chromosome synapsis,similar to phospholipase A2, involved incompletion of nuclear divisions during meiosis;induced early in meiosis 4e-050.00216 S000004838MRPL44YMR225CverifiedMitochondrial ribosomal protein of the large subunit 4e-050.00216 S000004289YHC1YLR298CverifiedComponent of the U1 snRNP complex required for pre-mRNAsplicing; putative ortholog of human U1Cprotein, which is involved in formation of acomplex between U1 snRNP and the pre-mRNA 5'splice site 4e-050.00216&radic S000002854ECM11YDR446WverifiedNon-essential protein apparently involved in meiosis, GFPfusion protein is present in discrete clustersin the nucleus throughout mitosis; may beinvolved in maintaining chromatin structure 4e-050.00216 S000002341LYS20YDL182WverifiedHomocitrate synthase isozyme, catalyzes the condensation ofacetyl-CoA and alpha-ketoglutarate to formhomocitrate, which is the first step in thelysine biosynthesis pathway; highly similar tothe other isozyme, Lys21p 4e-050.00216 S000000226POA1YBR022WverifiedPhosphatase that is highly specific for ADP-ribose1''-phosphate, a tRNA splicing metabolite; mayhave a role in regulation of tRNA splicing 4e-050.00216 S000000243ATP3YBR039WverifiedGamma subunit of the F1 sector of mitochondrial F1F0 ATPsynthase, which is a large, evolutionarilyconserved enzyme complex required for ATPsynthesis 4e-050.00216 S000000548PDI1YCL043CverifiedProtein disulfide isomerase, multifunctional protein residentin the endoplasmic reticulum lumen, essentialfor the formation of disulfide bonds insecretory and cell-surface proteins,unscrambles non-native disulfide bonds 4e-050.00216 S000005638CEX1YOR112WverifiedCytoplasmic component of the nuclear aminoacylation-dependenttRNA export pathway; interacts with nuclearpore component Nup116p; copurifies with tRNAexport receptors Los1p and Msn5p, as well aseIF-1a and the RAN GTPase Gsp1p 4e-050.00216 S000001419FMP35YIL157CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.00216 S000004880PPA2YMR267WverifiedMitochondrial inorganic pyrophosphatase, required formitochondrial function and possibly involved inenergy generation from inorganic pyrophosphate 4e-050.00216 S000006355SUE1YPR151CverifiedMitochondrial protein required for degradation of unstableforms of cytochrome c 4e-050.00216 S000000129RIB1YBL033CverifiedGTP cyclohydrolase II; catalyzes the first step of theriboflavin biosynthesis pathway 4e-050.00216 S000003448GPI1YGR216CverifiedMembrane protein involved in the synthesis ofN-acetylglucosaminyl phosphatidylinositol(GlcNAc-PI), the first intermediate in thesynthesis of glycosylphosphatidylinositol (GPI)anchors; human and mouse GPI1p are functionalhomologs 4e-050.00216 S000003968RPL8BYLL045CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Ap and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 4e-050.00216 S000004376IKI3YLR384CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;maintains structural integrity of Elongator;homolog of human IKAP, mutations in which causefamilial dysautonomia (FD) 4e-050.00216 S000002198NAT1YDL040CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 4e-050.00216 S000000834FIR1YER032WverifiedProtein involved in 3' mRNA processing, interacts with Ref2p;potential Cdc28p substrate 4e-050.00216 S000002831CAD1YDR423CverifiedAP-1-like bZIP transcriptional activator involved in multiplestress responses, iron metabolism, andpleiotropic drug resistance; controls a set ofgenes involved in stabilizing proteins, bindsconsensus sequence TTACTAA; 5' UTR containsuORFs 4e-050.00216&radic S000005425MET22YOL064CverifiedBisphosphate-3'-nucleotidase, involved in salt tolerance andmethionine biogenesis; dephosphorylates3'-phosphoadenosine-5'-phosphate and3'-phosphoadenosine-5'-phosphosulfate,intermediates of the sulfate assimilationpathway 4e-050.00216 S000004545DUS1YML080WverifiedDihydrouridine synthase, member of a widespread family ofconserved proteins including Smm1p, Dus3p, andDus4p; modifies pre-tRNA(Phe) at U17 4e-050.00216 S000001073RRM3YHR031CverifiedDNA helicase involved in rDNA replication and Ty1transposition; relieves replication fork pausesat telomeric regions; structurally andfunctionally related to Pif1p 4e-050.00216 S000004948PET8YNL003CverifiedS-adenosylmethionine transporter of the mitochondrial innermembrane, member of the mitochondrial carrierfamily; required for biotin biosynthesis andrespiratory growth 4e-050.00216 S000001116QNS1YHR074WverifiedGlutamine-dependent NAD(+) synthetase, essential for theformation of NAD(+) from nicotinic acid adeninedinucleotide 4e-050.00216 S000003300YGR068CUncharacterizedPutative protein of unknown function; YGR068C is not an essential gene. 4e-050.00216 S000001481YIR042CUncharacterizedPutative protein of unknown function; YIR042C is a non-essential gene 4e-050.00216 S000004095SEN2YLR105CverifiedSubunit of the tRNA splicing endonuclease, which is composed ofSen2p, Sen15p, Sen34p, and Sen54p; Sen2pcontains the active site for tRNA 5' splicesite cleavage and has similarity to Sen34p andto Archaeal tRNA splicing endonuclease 4e-050.00216 S000000917SPR6YER115CverifiedProtein of unknown function, expressed during sporulation; notrequired for sporulation, but gene exhibitsgenetic interactions with other genes requiredfor sporulation 4e-050.00216 S000003962UBI4YLL039CverifiedUbiquitin, becomes conjugated to proteins, marking them forselective degradation via the ubiquitin-26Sproteasome system; essential for the cellularstress response 4e-050.00216 S000004322CHS5YLR330WverifiedProtein involved in export from the Golgi to plasma membrane;involved in chitin biosynthesis through itsrole in Chs3p localization; interacts withArf1p, Bch1p, Fmp50p, Bud7p, and Chs6p 4e-050.00216 S000004882TMA23YMR269WverifiedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to thenucleolus; identified in proteomic screens ofribosomal complexes; YMR269W is not anessential gene 4e-050.00216 S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 4e-050.00216 S000000353ARA1YBR149WverifiedLarge subunit of NADP+ dependent arabinose dehydrogenase,involved in carbohydrate metabolism; smallsubunit is unidentified 4e-050.00216 S000005897MRS6YOR370CverifiedRab escort protein, forms a complex with the Ras-like smallGTPase Ypt1p that is required for theprenylation of Ypt1p by proteingeranylgeranyltransferase type II (Bet2p-Bet4p) 4e-050.00216 S000005817YOR291WUncharacterizedHypothetical protein 4e-050.00216 S000000746YEL020CUncharacterizedHypothetical protein with low sequence identity to Pdc1p 4e-050.00216 S000001763RHO4YKR055WverifiedNon-essential small GTPase of the Rho/Rac subfamily of Ras-likeproteins, likely to be involved in theestablishment of cell polarity 4e-050.00216 S000006064RPL33AYPL143WverifiedN-terminally acetylated ribosomal protein L37 of the large(60S) ribosomal subunit, nearly identical toRpl33Bp and has similarity to rat L35a; rpl33anull mutant exhibits slow growth while rpl33arpl33b double null mutant is inviable 4e-050.00216 S000000052GPB2YAL056WverifiedProposed beta subunit of the heterotrimeric G protein;interacts with the receptor Grp1p, affectssignaling by altering the level ofphosphorylation of PKS substrates; regulatespseudohyphal growth through cAMP levels;homolog of Gpb1p 4e-050.00216 S000004706MUB1YMR100WverifiedProtein of unknown function, deletion causes multi-buddingphenotype; has similarity to Aspergillusnidulans samB gene 4e-050.00216 S000005573STD1YOR047CverifiedProtein involved in control of glucose-regulated geneexpression; interacts with protein kinaseSnf1p, glucose sensors Snf3p and Rgt2p, andTATA-binding protein Spt15p; acts as aregulator of the transcription factor Rgt1p 4e-050.00216 S000005618ECM3YOR092WverifiedNon-essential protein of unknown function 4e-050.00216 S000003642IME2YJL106WverifiedSerine/threonine protein kinase involved in activation ofmeiosis, associates with Ime1p and mediates itsstability, activates Ndt80p; IME2 expression ispositively regulated by Ime1p 4e-050.00216 S000004210CCC1YLR220WverifiedPutative vacuolar Fe2+/Mn2+ transporter; suppresses respiratorydeficit of yfh1 mutants, which lack theortholog of mammalian frataxin, by preventingmitochondrial iron accumulation 4e-050.00216 S000003455HSV2YGR223CverifiedPhosphatidylinositol 3,5-bisphosphate-binding protein,predicted to fold as a seven-bladedbeta-propeller; displays punctate cytoplasmiclocalization 4e-050.00216 S000000672YCR076CUncharacterizedPutative protein of unknown function; YCR076C is not an essential gene 4e-050.00216 S000001522PTM1YKL039WverifiedProtein of unknown function, copurifies with late Golgivesicles containing the v-SNARE Tlg2p 4e-050.00216 S000005155YNL211CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene 4e-050.00216 S000002583NGG1YDR176WverifiedTranscriptional regulator involved in glucose repression ofGal4p-regulated genes; component oftranscriptional adaptor and histoneacetyltransferase complexes, the ADA complex,the SAGA complex, and the SLIK complex 4e-050.00216 S000000755BUD16YEL029CverifiedProtein involved in bud-site selection and telomeremaintenance; diploid mutants display a randombudding pattern instead of the wild-typebipolar pattern; has similarity to pyridoxalkinases 4e-050.00216 S000002527TRM1YDR120CverifiedtRNA methyltransferase, localizes to both the nucleus andmitochondrion to produce the modified baseN2,N2-dimethylguanosine in tRNAs in bothcompartments 4e-050.00216 S000004265SMD2YLR275WverifiedCore Sm protein Sm D2; part of heteroheptameric complex (withSmb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D2 4e-050.00216&radic S000004931PRE5YMR314Wverified20S proteasome alpha-type subunit 4e-050.00216 S000006166YPL245WUncharacterizedHypothetical protein 4e-050.00216 S000005402NGL1YOL042WverifiedPutative endonuclease, has a domain similar to amagnesium-dependent endonuclease motif in mRNAdeadenylase Ccr4p; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.00216 S000003011DST1YGL043WverifiedGeneral transcription elongation factor TFIIS, enables RNApolymerase II to read through blocks toelongation by stimulating cleavage of nascenttranscripts stalled at transcription arrestsites 4e-050.00216 S000005885HAP5YOR358WverifiedSubunit of the heme-activated, glucose-repressed Hap2/3/4/5CCAAT-binding complex, a transcriptionalactivator and global regulator of respiratorygene expression; required for assembly and DNAbinding activity of the complex 4e-050.00216 S000005467YOL107WUncharacterizedHypothetical protein 4e-050.00216 S000002762TRP4YDR354WverifiedAnthranilate phosphoribosyl transferase of the tryptophanbiosynthetic pathway, catalyzes thephosphoribosylation of anthranilate, subject tothe general control system of amino acidbiosynthesis 4e-050.00216 S000003137SUA5YGL169WverifiedProtein required for respiratory growth; null mutationsuppresses the Cyc1p translation defect causedby the presence of an aberrant ATG codonupstream of the correct start 4e-050.00216 S000003816HIT1YJR055WverifiedProtein of unknown function, required for growth at hightemperature 4e-050.0021 S000003226MNT2YGL257CverifiedMannosyltransferase involved in adding the 4th and 5th mannoseresidues of O-linked glycans 4e-050.0021 S000006168YPL247CUncharacterizedHypothetical protein 4e-050.0021 S000000240CSG2YBR036CverifiedEndoplasmic reticulum membrane protein, required formannosylation of inositolphosphorylceramide andfor growth at high calcium concentrations 4e-050.0021 S000006139SAR1YPL218WverifiedGTPase, GTP-binding protein of the ARF family, component ofCOPII coat of vesicles; required for transportvesicle formation during ER to Golgi proteintransport 4e-050.0021 S000003604YJL068CVerifiedNon-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D 4e-050.0021 S000004966HDA1YNL021WverifiedPutative catalytic subunit of a class II histone deacetylasecomplex that also contains Hda2p and Hda3p;Hda1p interacts with the Hda2p-Hda3p subcomplexto form an active tetramer; deletion increaseshistone H2B, H3 and H4 acetylation 4e-050.0021 S000003689INO1YJL153CverifiedInositol 1-phosphate synthase, involved in synthesis ofinositol phosphates and inositol-containingphospholipids; transcription is coregulatedwith other phospholipid biosynthetic genes byIno2p and Ino4p, which bind the UASINO DNAelement 4e-050.0021 S000004736POM152YMR129WverifiedNuclear pore membrane glycoprotein; may be involved induplication of nuclear pores and nuclear porecomplexes during S-phase; 4e-050.0021 S000005021APJ1YNL077WverifiedPutative chaperone of the HSP40 (DNAJ) family; overexpressioninterferes with propagation of the [Psi+]prion; the authentic, non-tagged protein isdetected in highly purified mitochondria inhigh-throughput studies 4e-050.0021 S000000453ARO4YBR249Cverified3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase,catalyzes the first step in aromatic amino acidbiosynthesis and is feedback-inhibited bytyrosine or high concentrations ofphenylalanine or tryptophan 4e-050.0021 S000000913SWI4YER111CverifiedDNA binding component of the SBF complex (Swi4p-Swi6p), atranscriptional activator that in concert withMBF (Mbp1-Swi6p) regulates late G1-specifictranscription of targets including cyclins andgenes required for DNA synthesis and repair 4e-050.0021 S000001684MNN4YKL201CverifiedPutative positive regulator of mannosylphosphate transferase(Mnn6p), involved in mannosylphosphorylation ofN-linked oligosaccharides; expression increasesin late-logarithmic and stationary growthphases 4e-050.0021 S000005686MTR10YOR160WverifiedNuclear import receptor, mediates the nuclear localization ofproteins involved in mRNA-nucleus export 4e-050.0021 S000000846ERG28YER044CverifiedEndoplasmic reticulum membrane protein, may facilitateprotein-protein interactions between the Erg26pdehydrogenase and the Erg27p 3-ketoreductaseand/or tether these enzymes to the ER, alsointeracts with Erg6p 4e-050.0021 S000006288YPR084WUncharacterizedHypothetical protein 4e-050.0021 S000003947SSA2YLL024CverifiedATP binding protein involved in protein folding and vacuolarimport of proteins; member of heat shockprotein 70 (HSP70) family; associated with thechaperonin-containing T-complex; present in thecytoplasm, vacuolar membrane and cell wall 4e-050.0021 S000002173TSC13YDL015CverifiedEnoyl reductase that catalyzes the last step in each cycle ofvery long chain fatty acid elongation,localizes to the ER, highly enriched in astructure marking nuclear-vacuolar junctions,coimmunoprecipitates with elongases Fen1p andSur4p 4e-050.0021 S000004747RIM11YMR139WverifiedProtein kinase required for signal transduction during entryinto meiosis; promotes the formation of theIme1p-Ume6p complex by phosphorylating Ime1pand Ume6p; shares similarity with mammalianglycogen synthase kinase 3-beta 4e-050.0021 S000003446RPS0AYGR214WverifiedProtein component of the small (40S) ribosomal subunit, nearlyidentical to Rps0Bp; required for maturation of18S rRNA along with Rps0Bp; deletion of eitherRPS0 gene reduces growth rate, deletion of bothgenes is lethal 4e-050.0021 S000001195SPO12YHR152WverifiedNucleolar protein of unknown function, positive regulator ofexit from mitosis; involved in regulating therelease of Cdc14p from the nucleolus in earlyanaphase; proposed to play similar role inmeiosis 4e-050.0021 S000003209DOC1YGL240WverifiedProcessivity factor required for the ubiquitination activity ofthe anaphase promoting complex (APC), mediatesthe activity of the APC by contributing tosubstrate recognition; involved in cyclinproteolysis 4e-050.0021 S000005687PNS1YOR161CverifiedProtein of unknown function; has similarity to Torpedocalifornica tCTL1p, which is postulated to be acholine transporter, neither null mutation noroverexpression affects choline transport 4e-050.0021 S000004212UTP13YLR222CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 4e-050.0021 S000000869YER067WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YER067W is not an essential gene 4e-050.0021 S000005991MUK1YPL070WuncharacterizedProtein of unknown function, localized to the cytoplasm;computational analysis of large-scaleprotein-protein interaction data suggests apossible role in transcriptional regulation 4e-050.0021 S000028597YBL071C-BUncharacterizedPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 4e-050.0021 S000000677SRB8YCR081WverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation; involved in glucoserepression 4e-050.0021 S000000597YCP4YCR004CverifiedProtein of unknown function, has sequence and structuralsimilarity to flavodoxins; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 4e-050.0021 S000006096SPT14YPL175WverifiedUDP-GlcNAc-binding and catalytic subunit of the enzyme thatmediates the first step inglycosylphosphatidylinositol (GPI)biosynthesis, mutations cause defects intranscription and in biogenesis of cell wallproteins 4e-050.0021 S000004296STT4YLR305CverifiedPhosphatidylinositol-4-kinase that functions in the Pkc1pprotein kinase pathway; required for normalvacuole morphology, cell wall integrity, andactin cytoskeleton organization 4e-050.0021 S000005516HXT11YOL156WverifiedPutative hexose transporter that is nearly identical to Hxt9p,has similarity to major facilitator superfamily(MFS) transporters and is involved inpleiotropic drug resistance 4e-050.0021 S000004764YMR155WUncharacterizedHypothetical protein 4e-050.0021 S000001469DCG1YIR030CverifiedProtein of unknown function, expression is sensitive tonitrogen catabolite repression and regulated byDal80p; contains transmembrane domain 4e-050.0021 S000001865CAK1YFL029CverifiedCyclin-dependent kinase-activating kinase required for passagethrough the cell cycle, phosphorylates andactivates Cdc28p; nucleotide-binding pocketdiffers significantly from those of most otherprotein kinases 4e-050.0021 S000003868YJR107WUncharacterizedPutative protein of unknown function; has sequence or structural similarity to lipases 4e-050.0021 S000001443DJP1YIR004WverifiedCytosolic J-domain-containing protein, required for peroxisomalprotein import and involved in peroxisomeassembly, homologous to E. coli DnaJ 4e-050.0021 S000000890DOT6YER088CverifiedProtein of unknown function, involved in telomeric genesilencing and filamentation 4e-050.0021 S000000772GLY1YEL046CverifiedThreonine aldolase, catalyzes the cleavage of L-allo-threonineand L-threonine to glycine; involved in glycinebiosynthesis 4e-050.0021 S000003626DPB11YJL090CverifiedEssential BRCT repeat protein, required on the prereplicativecomplex at replication origins for loading DNApolymerases to initiate DNA synthesis, alsorequired for S/M checkpoint control 4e-050.0021 S000001221STB5YHR178WverifiedActivator of multidrug resistance genes, forms a heterodimerwith Pdr1p; contains a Zn(II)2Cys6 zinc fingerdomain that interacts with a PDRE (pleotropicdrug resistance element) in vitro; binds Sin3pin a two-hybrid assay 4e-050.0021 S000004840TAF7YMR227CverifiedTFIID subunit (67 kDa), involved in RNA polymerase IItranscription initiation 4e-050.0021 S000006130IPL1YPL209CverifiedAurora kinase involved in regulating kinetochore-microtubuleattachments; helps maintain condensedchromosomes during anaphase and earlytelophase; associates with Sli15p, whichpromotes Ipl1p association with mitotic spindle 4e-050.0021 S000001282HIS6YIL020CverifiedPhosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamideisomerase, catalyzes the fourth step inhistidine biosynthesis; mutations causehistidine auxotrophy and sensitivity to Cu, Co,and Ni salts 4e-050.0021 S000002991PIB2YGL023CverifiedProtein binding phosphatidylinositol 3-phosphate, involved intelomere-proximal repression of geneexpression; similar to Fab1 and Vps27 4e-050.0021 S000000613PET18YCR020CverifiedProtein required for respiratory growth and stability of themitochondrial genome 4e-050.0021 S000004938FET4YMR319CverifiedLow-affinity Fe(II) transporter of the plasma membrane 4e-050.0021 S000005565CKB2YOR039WverifiedBeta' regulatory subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA1, CKA2 and CKB1, the many substratesinclude transcription factors and all RNApolymerases 4e-050.0021 S000002569NBP2YDR162CverifiedProtein involved in the HOG (high osmolarity glycerol) pathway,negatively regulates Hog1p by recruitment ofphosphatase Ptc1p the Pbs2p-Hog1p complex,found in the nucleus and cytoplasm, contains anSH3 domain that binds Pbs2p 4e-050.0021 S000000466FMP51YBR262CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.0021 S000002529KIN1YDR122WverifiedSerine/threonine protein kinase involved in regulation ofexocytosis; localizes to the cytoplasmic faceof the plasma membrane; closely related toKin2p 4e-050.0021 S000002993PGD1YGL025CverifiedSubunit of the Mediator global transcriptional cofactorcomplex, which is part of the RNA polymerase IIholoenzyme and plays an essential role in basaland activated transcription; direct target ofthe Cyc8p-Tup1p transcriptional corepressor 4e-050.0021 S000001056SPO13YHR014WverifiedMeiosis-specific protein, involved in maintaining sisterchromatid cohesion during meiosis I as well aspromoting proper attachment of kinetochores tothe spindle during meiosis I and meiosis II 4e-050.0021 S000001611PMU1YKL128CverifiedPutative phosphomutase, contains a region homologous to theactive site of phsophomutases; overexpressionof the gene suppresses the histidine auxotrophyof an ade3 ade16 ade17 triple mutant and thetemperature sensitivity of a tps2 mutant 4e-050.0021 S000002846THI74YDR438WuncharacterizedMitochondrial transporter repressible by thiamine 4e-050.0021 S000003880JHD2YJR119CuncharacterizedPutative protein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmand nucleus 4e-050.0021 S000000989YER187WUncharacterizedHypothetical protein 4e-050.0021 S000006032CAR1YPL111WverifiedArginase, responsible for arginine degradation, expressionresponds to both induction by arginine andnitrogen catabolite repression; disruptionenhances freeze tolerance 4e-050.0021 S000000252RPS11BYBR048WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps11Ap and has similarity to E.coli S17 and rat S11 ribosomal proteins 4e-050.0021 S000002290CDC53YDL132WverifiedCullin, structural protein of SCF complexes (which also containSkp1p, Cdc34p, and an F-box protein) involvedin ubiquitination; SCF promotes the G1-Stransition by targeting G1 cyclins and theCln-CDK inhibitor Sic1p for degradation 4e-050.0021 S000003977YLL054CUncharacterizedPutative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene 4e-050.0021 S000004550TUB1YML085CverifiedAlpha-tubulin; associates with beta-tubulin (Tub2p) to formtubulin dimer, which polymerizes to formmicrotubules 4e-050.0021 S000002435REG1YDR028CverifiedRegulatory subunit of type 1 protein phosphatase Glc7p,involved in negative regulation ofglucose-repressible genes 4e-050.0021 S000004604MIC17YMR002WverifiedMitochondrial intermembrane space cysteine motif protein; MIC17is not an essential gene 4e-050.0021 S000005742RUD3YOR216CverifiedGolgi matrix protein involved in the structural organization ofthe cis-Golgi; interacts genetically with COG3and USO1 4e-050.0021 S000005896RPS12YOR369CverifiedProtein component of the small (40S) ribosomal subunit; hassimilarity to rat ribosomal protein S12 4e-050.0021 S000003744NUC1YJL208CverifiedMajor mitochondrial nuclease, has RNAse and DNA endo- andexonucleolytic activities; has a role inmitochondrial recombination 4e-050.0021 S000001039ARN2YHL047CverifiedTransporter, member of the ARN family of transporters thatspecifically recognize siderophore-ironchelates; responsible for uptake of iron boundto the siderophore triacetylfusarinine C 4e-050.0021 S000006299SYT1YPR095CverifiedGuanine nucleotide exchange factor (GEF) for Arf proteins;involved in vesicular transport; suppressor ofypt3 mutations; member of the Sec7-domainfamily 4e-050.0021 S000000457SRB6YBR253WverifiedEssentital subunit of the RNA polymerase II mediator complex;associates with core polymerase subunits toform the RNA polymerase II holoenzyme;essential for transcriptional regulation 4e-050.0021 S000005216SEC2YNL272CverifiedGuanyl-nucleotide exchange factor for the small G-proteinSec4p, located on cytoplasmic vesicles;essential for post-Golgi vesicle transport 4e-050.0021 S000001928YFR032CUncharacterizedPutative protein of unknown function; transcribed during sporulation; YFR032C is not an essential gene 4e-050.0021 S000001484MET14YKL001CverifiedAdenylylsulfate kinase, required for sulfate assimilation andinvolved in methionine metabolism 4e-050.0021 S000000954YER152CUncharacterizedHypothetical protein 4e-050.0021 S000006370MRP2YPR166CverifiedMitochondrial ribosomal protein of the small subunit 4e-050.0021 S000004824DML1YMR211WverifiedEssential protein involved in mtDNA inheritance, may alsofunction in the partitioning of themitochondrial organelle or in the segregationof chromosomes, exhibits regions similar tomembers of a GTPase family 4e-050.0021 S000005054NOP15YNL110CverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis; localizes to bothnucleolus and cytoplasm 4e-050.0021 S000003400YGR168CUncharacterizedPutative protein of unknown function; YGR168C is not an essential gene 4e-050.0021 S000001004YHL012WUncharacterizedPutative protein of unknown function, has some homology to Ugp1p, which encodes UDP-glucose pyrophosphorylase 4e-050.0021 S000003007YGL039WVerifiedOxidoreductase, catalyzes NADPH-dependent reduction of the bicyclic diketone bicyclo[2.2.2]octane-2,6-dione (BCO2,6D) to the chiral ketoalcohol (1R,4S,6S)-6-hydroxybicyclo[2.2.2]octane-2-one (BCO2one6ol) 4e-050.0021 S000005071FAR11YNL127WverifiedProtein involved in G1 cell cycle arrest in response topheromone, in a pathway different from theFar1p-dependent pathway; interacts with Far3p,Far7p, Far8p, Far9p, and Far10p 4e-050.0021 S000005080EAF7YNL136WverifiedSubunit of the NuA4 histone acetyltransferase complex, whichacetylates the N-terminal tails of histones H4and H2A 4e-050.0021 S000001764TRM2YKR056WverifiedtRNA methyltransferase, 5-methylates the uridine residue atposition 54 of tRNAs and may also have a rolein tRNA stabilization or maturation; previouslythought to be an endo-exonuclease 4e-050.0021 S000000768GDA1YEL042WverifiedGuanosine diphosphatase located in the Golgi, involved in thetransport of GDP-mannose into the Golgi lumenby converting GDP to GMP after mannose istransferred its substrate 4e-050.0021 S000001577YJU3YKL094WverifiedSerine hydrolase with sequence similarity to monoglyceridelipase (MGL), localizes to lipid particles 4e-050.0021 S000007525YNR034W-AUncharacterizedHypothetical protein 4e-050.0021 S000001097CUP1-2YHR055CverifiedMetallothionein, binds copper and mediates resistance to highconcentrations of copper and cadmium; locus isvariably amplified in different strains, withtwo copies, CUP1-1 and CUP1-2, in the genomicsequence reference strain S288C 4e-050.0021 S000002180GPD1YDL022WverifiedNAD-dependent glycerol-3-phosphate dehydrogenase, key enzyme ofglycerol synthesis, essential for growth underosmotic stress; expression regulated byhigh-osmolarity glycerol response pathway;homolog of Gpd2p 4e-050.0021 S000005854SNC2YOR327CverifiedVesicle membrane receptor protein (v-SNARE) involved in thefusion between Golgi-derived secretory vesicleswith the plasma membrane; member of thesynaptobrevin/VAMP family of R-type v-SNAREproteins 4e-050.0021 S000006251MSF1YPR047WverifiedMitochondrial phenylalanyl-tRNA synthetase alpha subunit,active as a monomer, unlike the cytoplasmicsubunit which is active as a dimer complexed toa beta subunit dimer; similar to the alphasubunit of E. coli phenylalanyl-tRNA synthetase 4e-050.0021 S000001672HYM1YKL189WverifiedComponent of the RAM signaling network that is involved inregulation of Ace2p activity and cellularmorphogenesis, interacts with Kic1p and Sog2p,localizes to sites of polarized growth duringbudding and during the mating response 4e-050.0021 S000003791YJR030CUncharacterizedPutative protein of unknown function; expression repressed in carbon limited vs carbon replete chemostat cultures; YJR030C is a non-essential gene 4e-050.0021 S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 4e-050.0021 S000003110GPI10YGL142CverifiedIntegral membrane protein involved inglycosylphosphatidylinositol (GPI) anchorsynthesis; putative alpha 1,2mannosyltransferase required for addition ofthe third mannose onto the GPI core structure;human PIG-Bp is a functional homolog 4e-050.0021 S000006047NAN1YPL126WverifiedU3 snoRNP protein, component of the small (ribosomal) subunit(SSU) processosome containing U3 snoRNA;required for the biogenesis of18S rRNA 4e-050.0021 S000000232YBR028CUncharacterizedHypothetical protein 4e-050.0021 S000004908JNM1YMR294WverifiedComponent of the yeast dynactin complex, consisting of Nip100p,Jnm1p, and Arp1p; required for proper nuclearmigration and spindle partitioning duringmitotic anaphase B 4e-050.0021 S000004635HOF1YMR032WverifiedBud neck-localized, SH3 domain-containing protein required forcytokinesis; regulates actomyosin ring dynamicsand septin localization; interacts with theformins, Bni1p and Bnr1p, and with Cyk3p,Vrp1p, and Bni5p 4e-050.0021 S000005440REX4YOL080CverifiedPutative RNA exonuclease possibly involved in pre-rRNAprocessing and ribosome assembly 4e-050.0021 S000000099HTA2YBL003CverifiedOne of two nearly identical (see also HTA1) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 4e-050.0021 S000007616YKL106C-AUncharacterizedPutative protein of unknown function; identified by homology to uncharacterized proteins in other fungi 4e-050.0021 S000004911LCB1YMR296CverifiedComponent of serine palmitoyltransferase, responsible alongwith Lcb2p for the first committed step insphingolipid synthesis, which is thecondensation of serine with palmitoyl-CoA toform 3-ketosphinganine 4e-050.0021 S000003293ADE6YGR061CverifiedFormylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzesa step in the 'de novo' purine nucleotidebiosynthetic pathway 4e-050.0021 S000003944SPA2YLL021WverifiedComponent of the polarisome, which functions in actincytoskeletal organization during polarizedgrowth; acts as a scaffold for Mkk1p and Mpk1pcell wall integrity signaling components;potential Cdc28p substrate 4e-050.0021 S000005373HRD1YOL013CverifiedUbiquitin-protein ligase required for endoplasmicreticulum-associated degradation (ERAD) ofmisfolded proteins; genetically linked to theunfolded protein response (UPR); regulatedthrough association with Hrd3p; contains an H2ring finger 4e-050.0021 S000005918HSP33YOR391CuncharacterizedPossible chaperone and cysteine protease with similarity to E.coli Hsp31 and S. cerevisiae Hsp32p, Hsp33p,and Sno4p; member of the DJ-1/ThiJ/PfpIsuperfamily, which includes human DJ-1 involvedin Parkinson's disease 4e-050.0021 S000004329VRP1YLR337CverifiedProline-rich actin-associated protein involved in cytoskeletalorganization and cytokinesis; related tomammalian Wiskott-Aldrich syndrome protein(WASP)-interacting protein (WIP) 4e-050.0021 S000001724FMP13YKR016WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.0021 S000003089GPG1YGL121CverifiedProposed gamma subunit of the heterotrimeric G protein thatinteracts with the receptor Grp1p; involved inregulation of pseudohyphal growth; requiresGpb1p or Gpb2p to interact with Gpa2p 4e-050.0021 S000001791DAD2YKR083CverifiedEssential subunit of the Dam1 complex (aka DASH complex),couples kinetochores to the force produced byMT depolymerization thereby aiding inchromosome segregation; is transferred to thekinetochore prior to mitosis 4e-050.0021 S000001571YKL088WUncharacterizedPredicted phosphopantothenoylcysteine decarboxylase, may be involved in coenzyme A biosynthesis; interacts with Sis2p and Vhs3p 4e-050.0021 S000002432RPS11AYDR025WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps11Bp and has similarity to E.coli S17 and rat S11 ribosomal proteins 4e-050.0021 S000003333PCP1YGR101WverifiedMitochondrial serine protease required for the processing ofvarious mitochondrial proteins and maintenanceof mitochondrial DNA and morphology; belongs tothe rhomboid-GlpG superfamily of intramembranepeptidases 4e-050.0021 S000001351YIL089WUncharacterizedHypothetical protein 4e-050.0021 S000005108IBD2YNL164CverifiedComponent of the BUB2-dependent spindle checkpoint pathway,interacts with Bfa1p and functions upstream ofBub2p and Bfa1p 4e-050.0021 S000004710YPK2YMR104CverifiedProtein kinase with similarityto serine/threonine proteinkinase Ypk1p; functionally redundant with YPK1at the genetic level; participates in asignaling pathway required for optimal cellwall integrity; homolog of mammalian kinase SGK 4e-050.0021 S000002170YDL012CUncharacterizedPlasma membrane protein of unknown function; YDL012C is not an essential gene 4e-050.0021 S000006352YPR148CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 4e-050.0021 S000001152ERP5YHR110WverifiedProtein with similarity to Emp24p and Erv25p, member of the p24family involved in ER to Golgi transport 4e-050.0021 S000004099AHP1YLR109WverifiedThiol-specific peroxiredoxin, reduces hydroperoxides to protectagainst oxidative damage; function in vivorequires covalent conjugation to Urm1p 4e-050.0021 S000004460YPT7YML001WverifiedGTPase; GTP-binding protein of the rab family; required forhomotypic fusion event in vacuole inheritance,for endosome-endosome fusion, similar tomammalian Rab7 4e-050.0021 S000003941DPS1YLL018CverifiedCytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme thatcatalyzes the specific aspartylation oftRNA(Asp); class II aminoacyl tRNA synthetase;binding to its own mRNA may conferautoregulation 4e-050.0021 S000003636LSB6YJL100WverifiedPhosphatidylinositol 4-kinase that binds Las17p, which is ahomolog of human Wiskott-Aldrich Syndromeprotein involved in actin patch assembly andactin polymerization 4e-050.0021 S000003320CTT1YGR088WverifiedCytosolic catalase T, has a role in protection from oxidativedamage by hydrogen peroxide 4e-050.0021 S000005496PFK27YOL136Cverified6-phosphofructo-2-kinase, catalyzes synthesis offructose-2,6-bisphosphate; inhibited byphosphoenolpyruvate and sn-glycerol3-phosphate, expression induced by glucose andsucrose, transcriptional regulation involvesprotein kinase A 4e-050.0021 S000003297VHT1YGR065CverifiedHigh-affinity plasma membrane H+-biotin (vitamin H) symporter;mutation results in fatty acid auxotrophy; 12transmembrane domain containing majorfacilitator subfamily member; mRNA levelsnegatively regulated by iron deprivation andbiotin 4e-050.0021 S000004084GIS3YLR094CverifiedProtein of unknown function 4e-050.0021 S000005324COQ2YNR041CverifiedPara hydroxybenzoate: polyprenyl transferase, catalyzes thesecond step in ubiquinone (coenzyme Q)biosynthesis 4e-050.0021 S000003896MCM22YJR135CverifiedProtein involved in minichromosome maintenance; component ofthe kinetochore; binds to centromeric DNA in aCtf19p-dependent manner 4e-050.0021 S000005006GCD10YNL062CverifiedSubunit of tRNA (1-methyladenosine) methyltransferase withGcd14p, required for the modification of theadenine at position 58 in tRNAs, especiallytRNAi-Met; first identified as a negativeregulator of GCN4 expression 4e-050.0021 S000003612NET1YJL076WverifiedCore subunit of the RENT complex, which is a complex involvedin nucleolar silencing and telophase exit;stimulates transcription by RNA polymerase Iand regulates nucleolar structure 4e-050.0021 S000002822ERD1YDR414CverifiedPredicted membrane protein required for the retention oflumenal endoplasmic reticulum proteins; mutantssecrete the endogenous ER protein, BiP (Kar2p) 4e-050.0021 S000000845SAH1YER043CverifiedS-adenosyl-L-homocysteine hydrolase, catabolizesS-adenosyl-L-homocysteine which is formed afterdonation of the activated methyl group ofS-adenosyl-L-methionine (AdoMet) to an acceptor 4e-050.0021 S000003450CRM1YGR218WverifiedMajor karyopherin, involved in export of proteins, RNAs, andribosomal subunits from the nucleus 4e-050.0021 S000000157SKT5YBL061CverifiedActivator of Chs3p (chitin synthase III), recruits Chs3p to thebud neck via interaction with Bni4p; hassimilarity to Shc1p, which activates Chs3pduring sporulation 4e-050.0021 S000005257YNL313CUncharacterizedProtein required for cell viability 4e-050.0021 S000003955YLL032CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene 4e-050.0021&radic S000003240STF2YGR008CverifiedProtein involved in regulation of the mitochondrial F1F0-ATPsynthase; Stf1p and Stf2p act as stabilizingfactors that enhance inhibitory action of theInh1p protein 4e-050.0021 S000001201KEL1YHR158CverifiedProtein required for proper cell fusion and cell morphology;functions in a complex with Kel2p to negativelyregulate mitotic exit, interacts with Tem1p andLte1p; localizes to regions of polarizedgrowth; potential Cdc28p substrate 4e-050.0021 S000002873RMT2YDR465CverifiedArginine methyltransferase; ribosomal protein L12 is asubstrate 4e-050.0021 S000003675YUR1YJL139CverifiedMannosyltransferase of the KTR1 family, involved in proteinN-glycosylation; located in the Golgi apparatus 4e-050.0021 S000005017MSK1YNL073WverifiedMitochondrial lysine-tRNA synthetase, required for import ofboth aminoacylated and deacylated forms oftRNA(Lys) into mitochondria 4e-050.0021 S000002212MCH1YDL054CverifiedProtein with similarity to mammalian monocarboxylate permeases,which are involved in transport ofmonocarboxylic acids across the plasmamembrane; mutant is not deficient inmonocarboxylate transport 4e-050.0021 S000002336YDL177CUncharacterizedHypothetical protein 4e-050.0021 S000003039AFT1YGL071WverifiedTranscription factor involved in iron utilization andhomeostasis; binds the consensus sitePyPuCACCCPu and activates the expression oftarget genes in response to changes in ironavailability 4e-050.0021 S000002885SNF1YDR477WverifiedAMP-activated serine/threonine protein kinase found in acomplex containing Snf4p and members of theSip1p/Sip2p/Gal83p family; required fortranscription of glucose-repressed genes,thermotolerance, sporulation, and peroxisomebiogenesis 4e-050.0021 S000004020YLR030WUncharacterizedHypothetical protein 4e-050.0021 S000028534YBR196C-AUncharacterizedPutative protein of unknown function; identified by fungal homology and RT-PCR 4e-050.0021 S000001311DFG10YIL049WverifiedProtein of unknown function, involved in filamentous growth 4e-050.0021 S000000253REB1YBR049CverifiedRNA polymerase I enhancer binding protein; DNA binding proteinwhich binds to genes transcribed by both RNApolymerase I and RNA polymerase II; requiredfor termination of RNA polymerase Itranscription 4e-050.0021 S000006364GPH1YPR160WverifiedNon-essential glycogen phosphorylase required for themobilization of glycogen, activity is regulatedby cyclic AMP-mediated phosphorylation,expression is regulated by stress-responseelements and by the HOG MAP kinase pathway 4e-050.0021 S000003176SIP2YGL208WverifiedOne of three beta subunits of the Snf1 serine/threonine proteinkinase complex involved in the response toglucose starvation; null mutants exhibitaccelerated aging; N-myristoylprotein localizedto the cytoplasm and the plasma membrane 4e-050.0021 S000004261YLR271WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YLR271W is not an essential gene 4e-050.0021 S000005768SSP2YOR242CverifiedSporulation specific protein that localizes to the spore wall;required for sporulation at a point aftermeiosis II and during spore wall formation;SSP2 expression is induced midway in meiosis 4e-050.0021 S000004632RSF1YMR030WverifiedProtein required for respiratory growth; localized to both thenucleus and mitochondrion; mutant displaysdecreased transcription of specific nuclear andmitochondrial genes whose products are involvedin respiratory growth 4e-050.0021 S000006151YPL230WUncharacterizedPutative protein of unknown function 4e-050.0021 S000006234CSR2YPR030WverifiedNuclear protein with a potential regulatory role in utilizationof galactose and nonfermentable carbon sources;overproduction suppresses the lethality at hightemperature of a chs5 spa2 double nullmutation; potential Cdc28p substrate 4e-050.0021 S000007242YKL033W-AUncharacterizedPutative protein of unknown function; similar to uncharacterized proteins from other fungi 4e-050.0021 S000004875YMR262WUncharacterizedProtein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene 4e-050.0021 S000004410CDC73YLR418CverifiedConstituent of Paf1 complex with RNA polymerase II, Paf1p,Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinctfrom Srb-containing Pol II complexes; requiredfor expression of certain genes, modificationof some histones, and telomere maintenance 4e-050.0021 S000003839BNA2YJR078WverifiedTryptophan 2,3-dioxygenase, required for biosynthesis ofnicotinic acid from tryptophan via kynureninepathway 4e-050.0021 S000004046ERG3YLR056WverifiedC-5 sterol desaturase, catalyzes the introduction of a C-5(6)double bond into episterol, a precursor inergosterol biosynthesis; mutants are viable,but cannot grow on non-fermentable carbonsources 4e-050.0021 S000006119RPL7BYPL198WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl7Ap and has similarity to E.coli L30 and rat L7 ribosomal proteins;contains a conserved C-terminal Nucleic acidBinding Domain (NDB2) 4e-050.0021 S000003096CWC23YGL128CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to E. coliDnaJ and other DnaJ-like proteins and to S.pombe Cwf23p 4e-050.0021&radic S000000212FLR1YBR008CverifiedPlasma membrane multidrug transporter, member of the majorfacilitator superfamily; involved in efflux offluconazole, diazaborine, benomyl,methotrexate, and other drugs 4e-050.0021 S000005951YPL030WUncharacterizedHypothetical protein 4e-050.0021 S000004096MDN1YLR106CverifiedHuge dynein-related AAA-type ATPase (midasin), forms extendedpre-60S particle with the Rix1 complex(Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependentremodeling of 60S subunits and subsequentexport from nucleoplasm to cytoplasm 4e-050.0021 S000002560ENT5YDR153CverifiedProtein containing an N-terminal epsin-like domain involved inclathrin recruitment and traffic between theGolgi and endosomes; associates with theclathrin adaptor Gga2p, clathrin adaptorcomplex AP-1, and clathrin 4e-050.0021 S000000267YBR063CUncharacterizedPutative protein of unknown function; YBR063C is not an essential gene 4e-050.0021 S000005617TMA46YOR091WverifiedProtein of unknown function that associates with ribosomes;interacts with GTPase Rbg1p