Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0000393 "spliceosomal conformational changes to generate catalytic conformation"

GO branch: BP
Number of currently annotated genes: 8

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest TAP:161Huh:nucleusTAP:155Lee:REB1Harbison:DAL81_RAPAIPR007502PF02889Harbison:PHO4_YPDIPR002464PF04408 S000001794PRP16YKR086WverifiedRNA helicase in the DEAH-box family involved in the secondcatalytic step of splicing, exhibitsATP-dependent RNA unwinding activity &radic0.094930.7496&radic&radic&radic&radic S000000815PRP22YER013WverifiedDEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase,associates with lariat intermediates before thesecond catalytic step of splicing; mediatesATP-dependent mRNA release from the spliceosomeand unwinds RNA duplexes &radic0.0530.61678&radic&radic&radic&radic&radic&radic&radic S000003088PRP43YGL120CverifiedRNA helicase in the DEAH-box family, functions in both RNApolymerase I and polymerase II transcriptmetabolism, involved in release of thelariat-intron from the spliceosome 0.03990.56066&radic&radic&radic&radic&radic&radic S000005294PRP2YNR011CverifiedRNA-dependent ATPase in the DEAH-box family, required foractivation of the spliceosome before the firsttransesterification step in RNA splicing &radic0.037460.54776&radic&radic&radic&radic&radic&radic S000001208PRP8YHR165CverifiedComponent of the U4/U6-U5 snRNP complex, involved in the secondcatalytic step of splicing 0.029690.51939&radic&radic S000005835SNU66YOR308CverifiedComponent of the U4/U6.U5 snRNP complex involved in pre-mRNAsplicing via spliceosome; has homology to humanSART-1 and to an S. pombe protein; snu66 nullmutation confers cold-sensitivity but is notlethal at normal growth temperatures 0.028860.51323&radic&radic S000003959PRP19YLL036CverifiedSplicing factor associated with the spliceosome; contains aU-box, a motif found in a class of ubiquitinligases 0.022990.47148&radic&radic&radic S000002495SLU7YDR088CverifiedRNA splicing factor, required for ATP-independent portion of2nd catalytic step of spliceosomal RNAsplicing; interacts with Prp18p; contains zincknuckle domain &radic0.022820.47035&radic&radic S000001323SNP1YIL061CverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; may interact with poly(A)polymerase to regulate polyadenylation; homologof human U1 70K protein 0.020370.44363&radic&radic S000004107CLF1YLR117CverifiedEssential splicesome assembly factor; contains multipletetratricopeptide repeat (TPR) protein-bindingmotifs and interacts specifically with manyspliceosome components, may serve as a scaffoldduring splicesome assembly 0.020290.44188&radic&radic&radic S000003238PRP18YGR006WverifiedSplicing factor involved in the positioning of the 3' splicesite during the second catalytic step ofsplicing, recruited to the spliceosome by Slu7p &radic0.02020.43994&radic S000001656SNU114YKL173WverifiedGTPase component of U5 snRNP involved in mRNA splicing viaspliceosome; binds directly to U5 snRNA;proposed to be involved in conformationalchanges of the spliceosome; similarity toribosomal translocation factor EF-2 &radic0.019420.43592&radic&radic&radic&radic S000003323PRP31YGR091WverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.019480.43592&radic&radic S000002256SNU23YDL098CverifiedComponent of U4/U6.U5 snRNP involved in mRNA splicing viaspliceosome 0.019160.42969&radic&radic S000006382PRP4YPR178WverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.018560.42456&radic&radic S000002881PRP3YDR473CverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.018230.42188&radic&radic S000006386SMX3YPR182WverifiedCore Sm protein Sm F; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm F 0.018260.42188&radic&radic&radic S000000831SMB1YER029CverifiedCore Sm protein Sm B; part of heteroheptameric complex (withSmd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm B and Sm B' 0.018390.42188&radic&radic S000004853CUS1YMR240CverifiedProtein required for assembly of U2 snRNP into the spliceosome,forms a complex with Hsh49p and Hsh155p 0.018260.42188&radic&radic S000002786LSM6YDR378CverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.017930.42065&radic S000003306SMD1YGR074WverifiedCore Sm protein Sm D1; part of heteroheptameric complex (withSmb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D1 0.017690.42&radic&radic&radic S000004265SMD2YLR275WverifiedCore Sm protein Sm D2; part of heteroheptameric complex (withSmb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D2 0.017190.41511&radic&radic&radic S000006286DIB1YPR082Cverified17-kDa component of the U4/U6aU5 tri-snRNP, plays an essentialrole in pre-mRNA splicing, orthologue of hDIM1,the human U5-specific 15-kDa protein 0.016640.40286&radic S000000122LSM2YBL026WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.016490.40286&radic&radic S000000269ECM2YBR065CverifiedPre-mRNA splicing factor, facilitates the cooperative formationof U2/U6 helix II in association with stem IIin the spliceosome, function may be regulatedby Slu7p 0.015790.39437&radic&radic&radic S000000914LSM4YER112WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.015650.39437&radic&radic S000002965SMX2YFL017W-AverifiedCore Sm protein Sm G; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm G 0.014910.38415&radic&radic S000003307PRP38YGR075CverifiedUnique component of the U4/U6.U5 tri-snRNP particle,dispensable for spliceosome assembly, butrequired for conformational changes which leadto catalytic activation of the spliceosome 0.014790.38352&radic&radic S000000259PRP6YBR055CverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.014630.38248&radic&radic S000005091LSM7YNL147WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.014560.38223&radic S000004137SMD3YLR147CverifiedCore Sm protein Sm D3; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D3 0.014440.38072&radic S000004106MSL5YLR116WverifiedComponent of the commitment complex, which defines the firststep in the splicing pathway; essential proteinthat interacts with Mud2p and Prp40p, forming abridge between the intron ends; also involvedin nuclear retention of pre-mRNA 0.014510.38072&radic S000000948LSM5YER146WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.014340.37977&radic S000002368CWC2YDL209CverifiedProtein involved in pre-mRNA splicing, component of a complexcontaining Cef1p; interacts with Prp19p;contains an RNA recognition motif; hassimilarity to S. pombe Cwf2p 0.009780.32747&radic&radic&radic S000003739PRP21YJL203WverifiedSubunit of the SF3a splicing factor complex, required forspliceosome assembly 0.008860.3174&radic&radic&radic S000005685SME1YOR159CverifiedCore Sm protein Sm E; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm E 0.008190.30978&radic S000004826CEF1YMR213WverifiedEssential splicing factor; associated with Prp19p and thespliceosome, contains an N-terminal c-Myb DNAbinding motif necessary for cell viability butnot for Prp19p association, evolutionarilyconserved and homologous to S. pombe Cdc5p 0.007880.30332&radic&radic&radic S000006134LEA1YPL213WverifiedComponent of U2 snRNP; disruption causes reduced U2 snRNPlevels; physically interacts with Msl1p;invovled in telomere maintenance; putativehomolog of human U2A' snRNP protein 0.007880.30332&radic&radic&radic S000001448MSL1YIR009WverifiedU2B component of U2 snRNP, involved in splicing, binds the U2snRNA stem-loop IV in vitro; does not containthe conserved C-terminal RNA binding domainfound in other family members 0.007020.28447&radic&radic S000004901HSH155YMR288WverifiedU2-snRNP associated splicing factor that forms extensiveassociations with the branch site-3' splicesite-3' exon region upon prespliceosomeformation; similarity to the mammalian U2snRNP-associated splicing factor SAP155 0.006740.27688&radic&radic&radic S000002188PRP9YDL030WverifiedSubunit of the SF3a splicing factor complex, required forspliceosome assembly; acts after the formationof the U1 snRNP-pre-mRNA complex 0.006630.27452&radic&radic S000002245LUC7YDL087CverifiedEssential protein associated with the U1 snRNP complex;splicing factor involved in recognition of 5'splice site 0.006620.27339&radic&radic S000006305SNT309YPR101WverifiedComponent of NineTeen complex (NTC) containing Prp19p involvedin mRNA splicing, interacts physically andgenetically with Prp19p 0.006490.27152&radic&radic&radic S000002691GCN2YDR283CverifiedProtein kinase, phosphorylates the alpha-subunit of translationinitiation factor eIF2 (Sui2p) in response tostarvation; activated by uncharged tRNAs andthe Gcn1p-Gcn20p complex 0.006420.27025&radic S000003510CWC22YGR278WverifiedEssential protein, component of a complex containing Cef1p, hassimilarity to S. pombe Cwf22p; putativespliceosomal component, based on computationalanalysis of large-scale protein-proteininteraction data 0.006370.26613&radic&radic&radic S000004735ECM16YMR128WverifiedEssential DEAH-box ATP-dependent RNA helicase specific to theU3 snoRNP, predominantly nucleolar indistribution, required for 18S rRNA synthesis 0.006220.26429&radic&radic&radic&radic S000002651PRP28YDR243CverifiedRNA helicase in the DEAD-box family, involved in RNAisomerization at the 5' splice site 0.005710.24762&radic&radic S000003503SLH1YGR271WverifiedPutative RNA helicase related to Ski2p, involved in translationinhibition of non-poly(A) mRNAs; required forrepressing propagation of dsRNA viruses 0.00570.24762&radic S000001561DHR2YKL078WverifiedPredominantly nucleolar DEAH-box RNA helicase, required for 18SrRNA synthesis 0.005680.24643&radic&radic&radic S000004513RSE1YML049CverifiedProtein involved in pre-mRNA splicing; component of thepre-spliceosome; associates with U2 snRNA;involved in ER to Golgi transport 0.005540.24236&radic&radic S000004732STO1YMR125WverifiedLarge subunit of the nuclear mRNA cap-binding protein complex,interacts with Npl3p to carry nuclear poly(A)+mRNA to cytoplasm; also involved in nuclearmRNA degradation and telomere maintenance;orthologous to mammalian CBP80 0.005350.23394&radic&radic&radic S000004411YLR419WUncharacterizedPutative helicase with limited sequence similarity to human Rb protein; localized to the cytoplasm and the mitochondria; YLR419W is not an essential gene 0.005280.23263&radic&radic S000000974BRR2YER172CverifiedRNA-dependent ATPase RNA helicase involved in the facilitationand disruption of snRNA interactions, requiredfor disruption of U4/U6 base-pairing in nativesnRNPs to activate the spliceosome forcatalysis &radic0.005280.23263&radic&radic&radic S000003096CWC23YGL128CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to E. coliDnaJ and other DnaJ-like proteins and to S.pombe Cwf23p 0.005030.22205&radic&radic&radic S000002201PRP11YDL043CverifiedSubunit of the SF3a splicing factor complex, required forspliceosome assembly 0.004930.21839&radic&radic S000003245SNU71YGR013WverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; yeast specific, no metazoancounterpart 0.004780.21205&radic&radic S000006072PRP46YPL151CverifiedSplicing factor that is found in the Cef1p subcomplex of thespliceosome 0.004670.20757&radic&radic&radic S000001199LIN1YHR156CverifiedNon-essential component of U5 snRNP; nuclear protein;physically interacts with Irr1p of cohesincomplex; may link together proteins involved inchromosome segregation, mRNA splicing and DNAreplication 0.004660.20757&radic&radic S000003660LSM1YJL124CverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)involved in degradation of cytoplasmic mRNAs 0.004620.20538&radic S000004416SPP382YLR424WverifiedEssential protein that forms a dimer with Ntr2p; also forms atrimer, with Ntr2p and Prp43p, that is involvedin spliceosome disassembly; found also in amultisubunit complex with the splicing factorClf1p; suppressor of prp38-1 mutation 0.004530.20221&radic&radic&radic S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus 0.004250.19016&radic S000004509PRP39YML046WverifiedU1 snRNP protein involved in splicing, contains multipletetriatricopeptide repeats 0.004240.18817&radic&radic S000003427SKI6YGR195Wverified3'-to-5' phosphorolytic exoribonuclease that is a subunit ofthe exosome; required for 3' processing of the5.8S rRNA; involved in 3' to 5' mRNAdegradation and translation inhibition ofnon-poly(A) mRNAs 0.004110.18386&radic S000002824SYF1YDR416WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression; similar to Drosophila crooked neckprotein 0.004010.17772&radic&radic&radic S000000030PRP45YAL032CverifiedProtein required for pre-mRNA splicing; associates with thespliceosome and interacts with splicing factorsPrp22p and Prp46p; orthologous to humantranscriptional coactivator SKIP and canactivate transcription of a reporter gene 0.003960.1771&radic&radic S000000170AAR2YBL074CverifiedComponent of the U5 snRNP, required for splicing of U3precursors; originally described as a splicingfactor specifically required for splicingpre-mRNA of the MATa1 cistron 0.003850.17355&radic&radic S000001803MLP1YKR095WverifiedMyosin-like protein associated with the nuclear envelope,connects the nuclear pore complex with thenuclear interior; involved with Tel1p intelomere length control; involved with Pml1pand Pml39p in nuclear retention of unsplicedmRNAs 0.003790.17107 S000004059MEF1YLR069CverifiedMitochondrial elongation factor involved in translationalelongation 0.003570.1606 S000003141KEM1YGL173CverifiedEvolutionarily-conserved 5'-3' exonuclease component ofcytoplasmic processing (P) bodies involved inmRNA decay; plays a role inmicrotubule-mediated processes, filamentousgrowth, ribosomal RNA maturation, and telomeremaintenance 0.003540.15929 S000003220HFM1YGL251CverifiedMeiosis specific DNA helicase involved in the conversion ofdouble-stranded breaks to later recombinationintermediates and in crossover control;catalyzes the unwinding of Holliday junctions;has ssDNA and dsDNA stimulated ATPase activity 0.003480.15779 S000005659EFT1YOR133WverifiedElongation factor 2 (EF-2), also encoded by EFT2; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 0.003450.15693 S000006434LSM3YLR438C-AverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.00340.15517&radic S000002793EFT2YDR385WverifiedElongation factor 2 (EF-2), also encoded by EFT1; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 0.003360.15375 S000002772CDC40YDR364CverifiedPre-mRNA splicing factor, important for catalytic step II ofpre-mRNA splicing and plays a role in cellcycle progression; required for DNA synthesisduring mitosis and meiosis; has WD repeats 0.003320.15292&radic&radic S000005107RIA1YNL163CverifiedCytoplasmic GTPase involved in biogenesis of the 60S ribosome;has similarity to translation elongation factor2 (Eft1p and Eft2p) 0.003280.15152 S000002334AIR2YDL175CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p; may regulatemethylation of Npl3p, which modulates Npl3pfunction in mRNA processing and export; hassimilarity to Air1p 0.00320.14849&radic S000005199GIS2YNL255CverifiedProtein with seven cysteine-rich CCHC zinc-finger motifs,similar to human CNBP, proposed to be involvedin the RAS/cAMP signaling pathway 0.00310.14344 S000005674SPP2YOR148CverifiedEssential protein that promotes the first step of splicing andis required for the final stages of spliceosomematuration; interacts with Prp2p, which mayrelease Spp2p from the spliceosome followingthe first cleavage reaction 0.002940.13753&radic S000003783LSM8YJR022WverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)that is part of spliceosomal U6 snRNP and isalso implicated in processing of pre-tRNA,pre-snoRNA, and pre-rRNA 0.002940.13753&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p 0.002910.13656&radic S000000752SNU13YEL026WverifiedRNA binding protein, part of U3 snoRNP involved in rRNAprocessing, part of U4/U6-U5 tri-snRNP involvedin mRNA splicing, similar to human 15.5Kprotein 0.002790.13146 S000001732DBP7YKR024CverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in ribosomal biogenesis 0.002540.12145&radic S000005768SSP2YOR242CverifiedSporulation specific protein that localizes to the spore wall;required for sporulation at a point aftermeiosis II and during spore wall formation;SSP2 expression is induced midway in meiosis 0.002490.11903 S000004280GUF1YLR289WverifiedMitochondrial GTPase of unknown function, similar to E. colielongation factor-type GTP-binding protein LepAand to LK1236.1 from Caenorhabditis elegans 0.002370.11437 S000002242SUB2YDL084WverifiedComponent of the TREX complex required for nuclear mRNA export;member of the DEAD-box RNA helicase superfamilyand is involved in early and late steps ofspliceosome assembly; homolog of the humansplicing factor hUAP56 0.002350.11363&radic S000000939YER137CUncharacterizedHypothetical protein 0.002250.10982 S000003638MEF2YJL102WverifiedMitochondrial elongation factor involved in translationalelongation 0.002040.10028 S000002643PRP42YDR235WverifiedU1 snRNP protein involved in splicing, required for U1 snRNPbiogenesis; contains multipletetriatricopeptide repeats 0.002020.09997&radic&radic&radic S000001578YJU2YKL095WverifiedEssential nuclear protein; putative spliceosomal componentinvolved in mRNA splicing, based oncomputational analysis of large-scaleprotein-protein interaction data 0.001890.09432&radic&radic S000003767SUI2YJR007WverifiedAlpha subunit of the translation initiation factor eIF2,involved in the identification of the startcodon; phosphorylation of Ser51 is required forregulation of translation by inhibiting theexchange of GDP for GTP 0.001860.09349 S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif 0.001780.08874&radic&radic S000000356SPP381YBR152WverifiedmRNA splicing factor, component of U4/U6.U5 tri-snRNP;interacts genetically and physically withPrp38p &radic0.001770.08874&radic&radic&radic S000006261BRR1YPR057WverifiedsnRNP protein component of spliceosomal snRNPs, required forpre-mRNA splicing and snRNP biogenesis; in nullmutant newly-synthesized snRNAs aredestabilized and 3'-end processing is slowed 0.001750.0878&radic&radic&radic S000006139SAR1YPL218WverifiedGTPase, GTP-binding protein of the ARF family, component ofCOPII coat of vesicles; required for transportvesicle formation during ER to Golgi proteintransport 0.00170.08563 S000004842RRP5YMR229CverifiedProtein required for the synthesis of both 18S and 5.8S rRNA;C-terminal region is crucial for the formationof 18S rRNA and N-terminal region is requiredfor the 5.8S rRNA; component of small ribosomalsubunit (SSU) processosome 0.00170.08563 S000002812RPB7YDR404CverifiedRNA polymerase II subunit B16; forms two subunit dissociablecomplex with Rpb4p 0.001690.08524&radic S000001441MPH1YIR002CverifiedMember of the DEAH family of helicases, functions in anerror-free DNA damage bypass pathway thatinvolves homologous recombination, mutationsconfer a mutator phenotype 0.001670.08388&radic&radic&radic S000000467SHM1YBR263WverifiedMitochondrial serine hydroxymethyltransferase, involved inone-carbon metabolism 0.001590.07965 S000004802SGS1YMR190CverifiedNucleolar DNA helicase of the RecQ family involved inmaintenance of genome integrity, regulateschromosome synapsis and meiotic crossing over;has similarity to human BLM and WRN helicasesimplicated in Bloom and Werner syndromes 0.001540.07728&radic&radic S000002701SSD1YDR293CverifiedProtein with a role in maintenance of cellular integrity,interacts with components of the TOR pathway;ssd1 mutant of a clinical S. cerevisiae straindisplays elevated virulence 0.001540.07728 S000001664PRS1YKL181Wverified5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involvedin nucleotide, histidine, and tryptophanbiosynthesis; one of five related enzymes,which are active as heteromultimeric complexes 0.00150.07597 S000004431MRPL4YLR439WverifiedMitochondrial ribosomal protein of the large subunit 0.001490.07498&radic&radic S000006311YTH1YPR107CverifiedEssential RNA-binding component of cleavage and polyadenylationfactor, contains five zinc fingers; requiredfor pre-mRNA 3'-end processing andpolyadenylation 0.001440.07267&radic S000002435REG1YDR028CverifiedRegulatory subunit of type 1 protein phosphatase Glc7p,involved in negative regulation ofglucose-repressible genes 0.001430.0721 S000003149GTS1YGL181WverifiedProtein containing a zinc-finger in the N-terminus and a longGln-rich region in the C-terminus; regulatesultradian rhythm, cell size, cell cycle,lifespan, sporulation, heat tolerance, andmultidrug transport 0.001420.0721&radic S000004425CNA1YLR433CverifiedCalcineurin A; one isoform (the other is CMP2) of the catalyticsubunit of calcineurin, aCa++/calmodulin-regulated protein phosphatasewhich regulates Crz1p (a stress-responsetranscription factor), the other calcineurinsubunit is CNB1 0.001390.07 S000001042YHL050CUncharacterizedPutative protein of unknown function, potential Cdc28p substrate 0.001360.06888&radic S000005929CHL1YPL008WverifiedConserved nuclear protein required to establishsister-chromatid pairing during S-phase,probable DNA helicase with similarity to humanBACH1, which associates with tumor suppressorBRCA1; associates with acetyltransferase Ctf7p 0.001340.06679&radic&radic S000000318RAD16YBR114WverifiedProtein that recognizes and binds damaged DNA in anATP-dependent manner (with Rad7p) duringnucleotide excision repair; subunit ofNucleotide Excision Repair Factor 4 (NEF4);member of the SWI/SNF family 0.001310.06523&radic&radic S000002742SWR1YDR334WverifiedSwi2/Snf2-related ATPase that is the structural component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.001260.06293&radic&radic S000000966CHD1YER164WverifiedNucleosome remodeling factor that functions in regulation oftranscription elongation; contains a chromodomain, a helicase domain and a DNA-bindingdomain; component of both the SAGA and SILKcomplexes 0.001150.05577&radic&radic S000004881PRP24YMR268CverifiedSplicing factor that reanneals U4 and U6 snRNPs duringspliceosome recycling 0.001140.05512&radic&radic S000002648SNU56YDR240CverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; yeast specific, no metazoancounterpart; interacts with mRNA in commitmentcomplex 0.00110.05326&radic&radic S000001083SRB2YHR041CverifiedGeneral transcription factor, subunit of the RNA polymerase IImediator complex; associates with corepolymerase subunits to form the RNA polymeraseII holoenzyme; involved in telomere maintenance 0.00110.05299&radic&radic S000000973RAD3YER171Wverified5' to 3' DNA helicase, involved in nucleotide excision repairand transcription; subunit of RNA polymerase IItranscription initiation factor TFIIH; subunitof Nucleotide Excision Repair Factor 3 (NEF3);homolog of human XPD protein 0.00110.05299&radic&radic S000001495PRP40YKL012WverifiedU1 snRNP protein involved in splicing, interacts with thebranchpoint-binding protein during theformation of the second commitment complex 0.001080.05162&radic&radic S000003118INO80YGL150CverifiedATPase that forms a large complex, containing actin and severalactin-related proteins, that has chromatinremodeling activity and 3' to 5' DNA helicaseactivity in vitro; shows similarity to theSnf2p family of ATPases 0.001070.05162&radic&radic S000003704SET2YJL168CverifiedHistone methyltransferase with a role in transcriptionalelongation, methylates a lysine residue ofhistone H3; associates with the C-terminaldomain of Rpo21p; histone methylation activityis regulated by phosphorylation status ofRpo21p 0.001060.05053&radic S000005189CWC25YNL245CverifiedComponent of a complex containing Cef1p, involved in pre-mRNAsplicing; has similarity to S. pombe Cwf25p 0.001060.05053&radic S000004950MRP7YNL005CverifiedMitochondrial ribosomal protein of the large subunit 0.001070.05053&radic S000000805PMI40YER003CverifiedMannose-6-phosphate isomerase, catalyzes the interconversion offructose-6-P and mannose-6-P; required forearly steps in protein mannosylation 0.001060.05053&radic S000000323MUD1YBR119WverifiedU1 snRNP A protein, homolog of human U1-A; involved in nuclearmRNA splicing 0.001060.05053&radic&radic S000001653MRPL38YKL170WverifiedMitochondrial ribosomal protein of the large subunit; appearsas two protein spots (YmL34 and YmL38) ontwo-dimensional SDS gels 0.001060.05053&radic S000003599MRPL8YJL063CverifiedMitochondrial ribosomal protein of the large subunit 0.001050.05041&radic S000004401UTP21YLR409CuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.001050.05041&radic&radic S000001072SLT2YHR030CverifiedSerine/threonine MAP kinase involved in regulating themaintenance of cell wall integrity andprogression through the cell cycle; regulatedby the PKC1-mediated signaling pathway 0.001050.05019&radic S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.001050.05019 S000004626MRPL3YMR024WverifiedMitochondrial ribosomal protein of the large subunit 0.001050.05008&radic S000002870MRPL28YDR462WverifiedMitochondrial ribosomal protein of the large subunit 0.001030.04923&radic S000001877GNA1YFL017CverifiedEvolutionarily conserved glucosamine-6-phosphateacetyltransferase required for multiple cellcycle events including passage through START,DNA synthesis, and mitosis; involved inUDP-N-acetylglucosamine synthesis, formsGlcNAc6P from AcCoA 0.001030.04923&radic&radic S000002517FOB1YDR110WverifiedNucleolar protein required for DNA replication fork blockingand recombinational hotspot activities; bindsto the replication fork barrier site in therDNA region; related to retroviral integrases 0.001030.04873 S000004173TOS4YLR183CverifiedTranscription factor that binds to a number of promoterregions, particularly promoters of some genesinvolved in pheromone response and cell cycle;potential Cdc28p substrate; expression isinduced in G1 by bound SBF 0.001020.04873&radic&radic S000003506TAF1YGR274CverifiedTFIID subunit (145 kDa), involved in RNA polymerase IItranscription initiation, has histoneacetyltransferase activity, involved inpromoter binding and G1/S progression 0.001030.04873&radic&radic S000000818BIM1YER016WverifiedMicrotubule-binding protein that together with Kar9p makes upthe cortical microtubule capture site anddelays the exit from mitosis when the spindleis oriented abnormally 0.001020.04873 S000006132NIP7YPL211WverifiedNucleolar protein required for 60S ribosome subunit biogenesis,constituent of 66S pre-ribosomal particles;physically interacts with Nop8p and the exosomesubunit Rrp43p 0.001010.04843&radic S000000659BUD31YCR063WverifiedProtein involved in bud-site selection; analysis of integratedhigh-throughput datasets predicts aninvolvement in RNA splicing; diploid mutantsdisplay a random budding pattern instead of thewild-type bipolar pattern 0.0010.04616&radic&radic&radic S000002730MRPL35YDR322WverifiedMitochondrial ribosomal protein of the large subunit 0.0010.045&radic S000005119NOP13YNL175CverifiedProtein of unknown function, localizes to the nucleolus andnucleoplasm; contains an RNA recognition motif(RRM) and has similarity to Nop12p, which isrequired for processing of pre-18S rRNA 0.000980.045&radic S000005933RRP12YPL012WverifiedProtein required for export of the ribosomal subunits;associates with the RNA components of thepre-ribosomes; contains HEAT-repeats 0.000980.04488&radic S000003811ISY1YJR050WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing, auxiliary splicing factor that maymodulate Syf1p activity and help optimizesplicing; isy1 syf2 double mutation activatesthe spindle checkpoint, causing cell cyclearrest 0.000980.04451&radic&radic S000002303YDL144CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene. 0.000970.04441&radic S000004487YML6YML025CverifiedMitochondrial ribosomal protein of the large subunit, hassimilarity to E. coli L4 ribosomal protein andhuman mitoribosomal MRP-L4 protein; essentialfor viability, unlike most other mitoribosomalproteins 0.000970.04418&radic S000004422SEN1YLR430WverifiedPutative helicase required for RNA polymerase II transcriptiontermination and processing of RNAs; homolog ofSenataxin which causes Ataxia-OculomotorApraxia 2 and a dominant form of amyotrophiclateral sclerosis 0.000960.04383&radic S000000455MRPS5YBR251WverifiedMitochondrial ribosomal protein of the small subunit 0.000960.04383&radic S000002633HTA1YDR225WverifiedOne of two nearly identical (see also HTA2) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.000960.04383&radic S000005937SWI1YPL016WverifiedSubunit of the SWI/SNF chromatin remodeling complex, whichregulates transcription by remodelingchromosomes; required for transcription of manygenes, including ADH1, ADH2, GAL1, HO, INO1 andSUC2 0.000950.04318&radic S000005336NOG2YNR053CverifiedPutative GTPase that associates with pre-60S ribosomal subunitsin the nucleolus and is required for theirnuclear export and maturation 0.000960.04318&radic S000003202SEC15YGL233WverifiedEssential 113kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which mediates polarized targeting ofvesicles to active sites of exocytosis; Sec15passociates with Sec4p and vesicles 0.000940.04288 S000003502YTA7YGR270WverifiedProtein of unknown function, member of CDC48/PAS1/SEC18 familyof ATPases, potentially phosphorylated byCdc28p 0.000940.04288&radic S000003844CSN12YJR084WverifiedSubunit of the Cop9 signalosome, which is required fordeneddylation, or removal of the ubiquitin-likeprotein Rub1p from Cdc53p (cullin); involved inadaptation to pheromone signaling 0.000940.04266&radic&radic S000004618SOK2YMR016CverifiedNuclear protein that plays a regulatory role in the cyclic AMP(cAMP)-dependent protein kinase (PKA) signaltransduction pathway; negatively regulatespseudohyphal differentiation; homologous toseveral transcription factors 0.000940.04266&radic S000003829RFC2YJR068WverifiedSubunit of heteropentameric Replication factor C (RF-C), whichis a DNA binding protein and ATPase that actsas a clamp loader of the proliferating cellnuclear antigen (PCNA) processivity factor forDNA polymerases delta and epsilon 0.000940.04266&radic S000002726MCM21YDR318WverifiedProtein involved in minichromosome maintenance; component ofthe COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p)that bridges kinetochore subunits that are incontact with centromeric DNA and the subunitsbound to microtubules 0.000930.04224&radic S000006156RVB2YPL235WverifiedEssential protein involved in transcription regulation;component of chromatin remodeling complexes;required for assembly and function of the INO80complex; member of the RUVB-like protein family 0.000930.04224&radic S000004289YHC1YLR298CverifiedComponent of the U1 snRNP complex required for pre-mRNAsplicing; putative ortholog of human U1Cprotein, which is involved in formation of acomplex between U1 snRNP and the pre-mRNA 5'splice site 0.000930.04224&radic&radic S000000642IMG1YCR046CverifiedMitochondrial ribosomal protein of the large subunit, requiredfor respiration and for maintenance of themitochondrial genome 0.000930.04224&radic S000001456MET28YIR017CverifiedTranscriptional activator in the Cbf1p-Met4p-Met28p complex,participates in the regulation of sulfurmetabolism 0.00090.0411&radic S000002319DHH1YDL160CverifiedCytoplasmic DExD/H-box helicase, stimulates mRNA decapping,coordinates distinct steps in mRNA function anddecay, interacts with both the decapping anddeadenylase complexes, may have a role in mRNAexport and translation 0.00090.04097&radic S000004619SPO20YMR017WverifiedMeiosis-specific subunit of the t-SNARE complex, required forprospore membrane formation during sporulation;similar to but not functionally redundant withSec9p; SNAP-25 homolog 0.00090.04097 S000002172NOP1YDL014WverifiedNucleolar protein, component of the small subunit processomecomplex, which is required for processing ofpre-18S rRNA; has similarity to mammalianfibrillarin 0.00090.04093 S000005299ACC1YNR016CverifiedAcetyl-CoA carboxylase, biotin containing enzyme that catalyzesthe carboxylation of acetyl-CoA to formmalonyl-CoA; required for de novo biosynthesisof long-chain fatty acids 0.000890.04054 S000003068SEH1YGL100WverifiedNuclear pore protein that is part of the evolutionarilyconserved Nup84p complex (Nup84p, Nup85p,Nup120p, Nup145p, and Seh1p); homologous toSec13p 0.00090.04054 S000005132KAR1YNL188WverifiedEssential protein involved in karyogamy during mating and inspindle pole body duplication during mitosis,localizes to the half-bridge of the spindlepole body, interacts with Spc72p duringkaryogamy, also interacts with Cdc31p 0.00090.04054 S000001901SAD1YFR005CverifiedConserved zinc-finger domain protein involved in pre-mRNAsplicing, required for assembly of U4 snRNAinto the U4/U6 particle 0.000890.04044&radic&radic S000003318PIL1YGR086CverifiedPrimary component of eisosomes, which are large immobile patchstructures at the cell cortex associated withendocytosis, along with Lsp1p and Sur7p; nullmutants show activation of Pkc1p/Ypk1p stressresistance pathways 0.000890.04039 S000002367NHP2YDL208WverifiedNuclear protein related to mammalian high mobility group (HMG)proteins, essential for function of H/ACA-typesnoRNPs, which are involved in 18S rRNAprocessing 0.000880.04006 S000003464NAS6YGR232WverifiedRegulatory, non-ATPase subunit of the 26S proteasome; homologof the human oncoprotein gankyrin, whichinteracts with the retinoblastoma tumorsuppressor (Rb) and cyclin-dependent kinase 4/6 0.000880.03996&radic&radic S000000200YBL104CUncharacterizedHypothetical protein 0.000880.03996 S000001195SPO12YHR152WverifiedNucleolar protein of unknown function, positive regulator ofexit from mitosis; involved in regulating therelease of Cdc14p from the nucleolus in earlyanaphase; proposed to play similar role inmeiosis 0.000860.03951&radic S000006162CIN2YPL241CverifiedTubulin folding factor C (putative) involved in beta-tubulin(Tub2p) folding; isolated as mutant withincreased chromosome loss and sensitivity tobenomyl 0.000860.03951 S000002280UBP1YDL122WverifiedUbiquitin-specific protease that removes ubiquitin fromubiquitinated proteins; cleaves at the Cterminus of ubiquitin fusions irrespective oftheir size; capable of cleaving polyubiquitinchains 0.000860.03938 S000001337RPN2YIL075CverifiedSubunit of the 26S proteasome, substrate of theN-acetyltransferase Nat1p 0.000850.03895&radic&radic S000005499CDC33YOL139CverifiedCytoplasmic mRNA cap binding protein; the eIF4E-cap complex isresponsible for mediating cap-dependent mRNAtranslation via interactions with thetranslation initiation factor eIF4G (Tif4631por Tif4632p) 0.000850.03895&radic S000004929ELP6YMR312WverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;required for Elongator structural integrity 0.000840.0381 S000005502RRP40YOL142WverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.000840.0381&radic S000000967PAB1YER165WverifiedPoly(A) binding protein, part of the 3'-end RNA-processingcomplex, mediates interactions between the 5'cap structure and the 3' mRNA poly(A) tail,involved in control of poly(A) tail length,interacts with translation factor eIF-4G 0.000840.0381 S000004607TAF4YMR005WverifiedTFIID subunit (48 kDa), involved in RNA polymerase IItranscription initiation; potential Cdc28psubstrate 0.000820.03767 S000007588YDL085C-AUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.000820.03767&radic S000000033FUN12YAL035WverifiedGTPase, required for general translation initiation bypromoting Met-tRNAiMet binding to ribosomes andribosomal subunit joining; homolog of bacterialIF2 0.000810.03719 S000001915FAB1YFR019Wverified1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membranekinase that generates phosphatidylinositol(3,5)P2, which is involved in vacuolar sortingand homeostasis 0.000810.03719 S000003851JSN1YJR091CverifiedMember of the Puf family of RNA-binding proteins, interactswith mRNAs encoding membrane-associatedproteins; overexpression suppresses a tub2-150mutation and causes increased sensitivity tobenomyl in wild-type cells 0.000790.03639 S000001128NAM8YHR086WverifiedRNA binding protein, component of the U1 snRNP protein; mutantsare defective in meiotic recombination and information of viable spores, involved in theformation of DSBs through meiosis-specificsplicing of MER2 pre-mRNA 0.000780.03577&radic S000004301CDC25YLR310CverifiedMembrane bound guanine nucleotide exchange factor (GEF orGDP-release factor); indirectly regulatesadenylate cyclase through activation of Ras1pand Ras2p by stimulating the exchange of GDPfor GTP; required for progression through G1 0.000770.03565&radic S000001065MYO1YHR023WverifiedType II myosin heavy chain, required for wild-type cytokinesisand cell separation; localizes to theactomyosin ring; binds to myosin light chainsMlc1p and Mlc2p through its IQ1 and IQ2 motifsrespectively 0.000770.03536 S000006026YPL105CUncharacterizedHypothetical protein 0.000760.03507 S000003432ELP2YGR200CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;target of Kluyveromyces lactis zymocin 0.000750.03483 S000006161HSP82YPL240CverifiedCytoplasmic chaperone (Hsp90 family) required for pheromonesignaling and negative regulation of Hsf1p;docks with the mitochondrial import receptorTom70p for preprotein delivery; interacts withco-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p 0.000720.03347 S000000029GIP4YAL031CverifiedCytoplasmic Glc7-interacting protein whose overexpressionrelocalizes Glc7p from the nucleus and preventschromosome segregation; potential Cdc28psubstrate 0.000730.03347&radic S000006022ELP4YPL101WverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;required for Elongator structural integrity 0.000710.03291 S000006284TEF1YPR080WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF2; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.000670.03156 S000000449ISW1YBR245CverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPase thatforms a complex with Ioc2p and Ioc4p toregulate transcription elongation, and acomplex with Ioc3p to repress transcriptioninitiation 0.000640.03043&radic&radic S000005383IFM1YOL023WverifiedMitochondrial translation initiation factor 2 0.000640.03043 S000002953YRF1-1YDR545WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.000590.02841 S000003452MRPL9YGR220CverifiedMitochondrial ribosomal protein of the large subunit 0.000590.02841 S000002151YBL111CUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.000570.02725 S000000827GCD11YER025WverifiedGamma subunit of the translation initiation factor eIF2,involved in the identification of the startcodon; binds GTP when forming the ternarycomplex with GTP and tRNAi-Met 0.000570.02724 S000003139ROK1YGL171WverifiedATP-dependent RNA helicase of the DEAD box family; required for18S rRNA synthesis 0.000560.02682 S000002602MSS116YDR194CverifiedDEAD-box protein required for efficient splicing ofmitochondrial Group I and II introns; non-polarRNA helicase that also facilities strandannealing 0.000560.02682 S000005589RPL3YOR063WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to E. coli L3 and rat L3 ribosomalproteins; involved in the replication andmaintenance of killer double stranded RNA virus 0.000550.02659 S000001212DBP8YHR169WverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in biogenesis of the 40S ribosomalsubunit 0.000550.0265&radic S000004602YML133CUncharacterizedPutative protein of unknown function with similarity to helicases; localized to mitochondria; YML133C contains an intron 0.000550.02625 S000007526YOR396WUncharacterizedHypothetical protein 0.000540.0261 S000002579SUP35YDR172WverifiedTranslation termination factor eRF3; altered proteinconformation creates the [PSI(+)] prion, adominant cytoplasmically inherited proteinaggregate that alters translational fidelityand creates a nonsense suppressor phenotype 0.000530.02579 S000004458YRF1-4YLR466WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.000530.02579 S000003990YLL067CUncharacterizedPutative protein of unknown function with similarity to helicases 0.000520.02512 S000003528YRF1-3YGR296WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.000510.0246 S000001894SPB4YFL002CverifiedPutative ATP-dependent RNA helicase, nucleolar protein requiredfor synthesis of 60S ribosomal subunits at alate step in the pathway; sediments with 66Spre-ribosomes in sucrose gradients 0.00050.02406&radic S000002699HRQ1YDR291WuncharacterizedPutative DNA helicase 0.000490.02252&radic S000000992YRF1-2YER190WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.00050.02252 S000006409YEL077CUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.00050.02252 S000003989YLL066CUncharacterizedPutative protein of unknown function with similarity to helicases; YLL066C is not an essential gene 0.00050.02252 S000000441PRP5YBR237WverifiedRNA helicase in the DEAD-box family, necessary forprespliceosome formation, bridges U1 and U2snRNPs and enables stable U2 snRNP associationwith intron RNA 0.000490.02236&radic&radic S000006204YRF1-7YPL283CverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.000480.02211 S000003674TIF2YJL138CverifiedTranslation initiation factor eIF4A, identical to Tif1p;DEA(D/H)-box RNA helicase that couples ATPaseactivity to RNA binding and unwinding; forms adumbbell structure of two compact domainsconnected by a linker; interacts with eIF4G 0.000480.02138 S000005730DED1YOR204WverifiedATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, requiredfor translation initiation of all yeast mRNAs;mutations in human DEAD-box DBY are a frequentcause of male infertility 0.000470.02053 S000004903HAS1YMR290CverifiedATP-dependent RNA helicase; localizes to both the nuclearperiphery and nucleolus; highly enriched innuclear pore complex fractions; constituent of66S pre-ribosomal particles 0.000460.01984 S000001828YFL066CUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.000450.01935 S000004390SKI2YLR398CverifiedPutative RNA helicase, involved in exosome mediated 3' to 5'mRNA degradation and translation inhibition ofnon-poly(A) mRNAs; forms complex with Ski3p andSki8p; required for repressing propagation ofdsRNA viruses 0.000450.01935 S000005283YRF1-6YNL339CverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.000450.01934 S000001767TIF1YKR059WverifiedTranslation initiation factor eIF4A, identical to Tif2p;DEA(D/H)-box RNA helicase that couples ATPaseactivity to RNA binding and unwinding; forms adumbbell structure of two compact domainsconnected by a linker; interacts with eIF4G 0.000450.01934 S000001107RRP3YHR065CverifiedProtein involved in rRNA processing; required for maturation ofthe 35S primary transcript of pre-rRNA and forcleavage leading to mature 18S rRNA; homologousto eIF-4a, which is a DEAD box RNA-dependentATPase with helicase activity 0.000450.01915 S000003570HCA4YJL033WverifiedPutative nucleolar DEAD box RNA helicase; high-copy numbersuppression of a U14 snoRNA processing mutantsuggests an involvement in 18S rRNA synthesis 0.000440.01907 S000004266DBP9YLR276CverifiedATP-dependent RNA helicase of the DEAD-box family involved inbiogenesis of the 60S ribosomal subunit 0.000440.0189 S000004237IRC20YLR247CuncharacterizedPutative helicase; localized to mitochondria and the nucleus;YLR247C is not an essential gene; null mutantdisplays increased levels of spontaneous Rad52foci 0.000430.01861&radic S000005056DBP2YNL112WverifiedEssential ATP-dependent RNA helicase of the DEAD-box proteinfamily, involved in nonsense-mediated mRNAdecay and rRNA processing 0.000420.01847&radic S000001405SSL2YIL143CverifiedComponent of the holoenzyme form of RNA polymerasetranscription factor TFIIH, has DNA-dependentATPase/helicase activity and is required, withRad3p, for unwinding promoter DNA; involved inDNA repair; homolog of human ERCC3 0.000420.01839&radic S000002189DBP10YDL031WverifiedPutative ATP-dependent RNA helicase of the DEAD-box proteinfamily, constituent of 66S pre-ribosomalparticles; essential protein involved inribosome biogenesis 0.000420.01831 S000000346MAK5YBR142WverifiedEssential nucleolar protein, putative DEAD-box RNA helicaserequired for maintenance of M1 dsRNA virus;involved in biogenesis of large (60S) ribosomalsubunits 0.000420.01831&radic S000005950SUV3YPL029WverifiedATP-dependent RNA helicase, component of the mitochondrialdegradosome along with the RNase Dss1p; thedegradosome associates with the ribosome andmediates turnover of aberrant or unprocessedRNAs 0.000410.01781 S000003131RAD54YGL163CverifiedDNA-dependent ATPase, stimulates strand exchange by modifyingthe topology of double-stranded DNA; involvedin the recombinational repair of double-strandbreaks in DNA during vegetative growth andmeiosis; member of the SWI/SNF family 0.000410.01754&radic S000003586MTR4YJL050WverifiedDead-box family ATP dependent helicase required for mRNA exportfrom the nucleus; co-factor of the exosomecomplex, required for 3' end formation of 5.8SrRNA 0.000410.01722&radic S000006003MOT1YPL082CverifiedEssential abundant protein involved in regulation oftranscription, removes Spt15p (TBP) from DNAvia its C-terminal ATPase activity, forms acomplex with TBP that binds TATA DNA with highaffinity but with altered specificity 0.000410.01722&radic S000000017FUN30YAL019WverifiedProtein whose overexpression affects chromosome stability,potential Cdc28p substrate; homolog of Snf2p;the authentic, non-tagged protein is detectedin highly purified mitochondria inhigh-throughput studies 0.00040.01671&radic S000003796RAD26YJR035WverifiedProtein involved in transcription-coupled repair nucleotideexcision repair of UV-induced DNA lesions;homolog of human CSB protein 0.00040.01671&radic S000002428FAL1YDR021WverifiedNucleolar protein required for maturation of 18S rRNA, memberof the eIF4A subfamily of DEAD-boxATP-dependent RNA helicases 0.00040.01652&radic S000001934IRC5YFR038WuncharacterizedPutative ATPase containing the DEAD/H helicase-related sequencemotif; null mutant displays increased levels ofspontaneous Rad52 foci 0.000390.01537 S000002740IRC3YDR332WuncharacterizedHypothetical protein; null mutant displays increased levels ofspontaneous Rad52 foci 0.000380.01452&radic S000001439YIL177CUncharacterizedPutative protein of unknown function; similarity to DNA helicases that are encoded within subtelomeric Y' elements and induced in telomerase deficient survivors 0.000370.01408 S000006040DBP1YPL119CverifiedPutative ATP-dependent RNA helicase of the DEAD-box proteinfamily; mutants show reduced stability of the40S ribosomal subunit scanning through 5'untranslated regions of mRNAs 0.000380.01408 S000004459YRF1-5YLR467WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.000370.0138 S000003032MRH4YGL064CverifiedMitochondrial RNA helicase of the DEAD-box family, plays anessential role in mitochondrial function 0.000350.01243 S000005321DBP6YNR038WverifiedEssential protein involved in ribosome biogenesis; putativeATP-dependent RNA helicase of the DEAD-boxprotein family 0.000330.01128&radic S000003046DBP3YGL078CverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in ribosomal biogenesis 0.000320.01084&radic S000001261YHR218WUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.000290.00789&radic S000001179ARO9YHR137WverifiedAromatic aminotransferase, catalyzes the first step oftryptophan, phenylalanine, and tyrosinecatabolism 0.000250.00521&radic S000002634ADK1YDR226WverifiedAdenylate kinase, required for purine metabolism; localized tothe cytoplasm and the mitochondria; lackscleavable signal sequence 0.000250.00489&radic S000002537FIN1YDR130CverifiedSpindle pole body-related intermediate filament protein, formscell cycle-specific filaments between spindlepole bodies in mother and daughter cells, ableto self-assemble, expression induced duringS/G2, localization cell-cycle dependent 0.000240.00468&radic S000005711GSP2YOR185CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; interacts withKap121p, Kap123p and Pdr6p (karyophilin betas);Gsp1p homolog that is not required forviability 0.000240.00468&radic S000001017SNF6YHL025WverifiedSubunit of the SWI/SNF chromatin remodeling complex involved intranscriptional regulation; functionsinterdependently in transcriptional activationwith Snf2p and Snf5p 0.000240.00455&radic S000000869YER067WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YER067W is not an essential gene 0.000240.0045&radic S000006373JIP5YPR169WuncharacterizedNucleolar protein of unknown function, exhibits a physicalinteraction with Bre1p 0.000240.0045&radic&radic S000001163LSM12YHR121WverifiedProtein of unknown function that interacts with Pbp1p and Pbp4pand associates with ribosomes; contains anRNA-binding LSM domain and an AD domain; mayplay a role in RNA processing 0.000240.00442&radic S000002480SNF11YDR073WverifiedSubunit of the SWI/SNF chromatin remodeling complex involved intranscriptional regulation; interacts with ahighly conserved 40-residue sequence of Snf2p 0.000240.0043&radic S000000419HPC2YBR215WverifiedHighly charged, basic protein required for normal cell-cycleregulation of histone gene transcription;mutants display strong synthetic defects withsubunits of FACT, a complex that allows RNA PolII to elongate through nucleosomes 0.000240.0043&radic S000000213HHF1YBR009CverifiedOne of two identical histone H4 proteins (see also HHF2); corehistone required for chromatin assembly andchromosome function; contributes to telomericsilencing; N-terminal domain involved inmaintaining genomic integrity 0.000240.0043&radic S000002817SIZ1YDR409WverifiedSUMO ligase that promotes the attachment of sumo (Smt3p; smallubiquitin-related modifier) to proteins; bindsUbc9p and may bind septins; specificallyrequired for sumoylation of septins in vivo;localized to the septin ring 0.000240.00428&radic S000002895RIB3YDR487Cverified3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase),required for riboflavin biosynthesis fromribulose-5-phosphate, also has an unrelatedfunction in mitochondrial respiration 0.000240.00428&radic S000003178YPT32YGL210WverifiedGTPase of the Ypt/Rab family, very similar to Ypt31p; involvedin the exocytic pathway; mediates intra-Golgitraffic or the budding of post-Golgi vesiclesfrom the trans-Golgi 0.000240.00418&radic S000002921GRX2YDR513WverifiedCytoplasmic glutaredoxin, thioltransferase,glutathione-dependent disulfide oxidoreductaseinvolved in maintaining redox state of targetproteins, also exhibits glutathione peroxidaseactivity, expression induced in response tostress 0.000240.00418&radic S000003550MAD3YJL013CverifiedComponent of the spindle-assembly checkpoint complex, whichdelays the onset of anaphase in cells withdefects in mitotic spindle assembly; interactsphysically with the spindle checkpoint proteinsBub3p and Mad2p 0.000240.00418&radic S000001866CAF16YFL028CverifiedPart of the evolutionarily-conserved CCR4-NOT transcriptionalregulatory complex involved in controlling mRNAinitiation, elongation, and degradation;putative ABC ATPase; interacts with Ssn2p,Ssn3p, and Ssn8p 0.000240.00418&radic S000004523SML1YML058WverifiedRibonucleotide reductase inhibitor involved in regulating dNTPproduction; regulated by Mec1p and Rad53pduring DNA damage and S phase 0.000240.00418&radic S000003175SPT16YGL207WverifiedSubunit of the heterodimeric FACT complex (Spt16p-Pob3p),facilitates RNA Polymerase II transcriptionelongation through nucleosomes by destabilizingand then reassembling nucleosome structure 0.000240.00418&radic S000002820RRP17YDR412WuncharacterizedProtein required for cell viability 0.000240.00418&radic&radic S000000971RPH1YER169WverifiedTranscriptional repressor of PHR1, which is a photolyaseinduced by DNA damage; binds to AG(4) (C(4)T)sequence upstream of PHR1; Rph1pphosphorylation during DNA damage is undercontrol of the MEC1-RAD53 pathway 0.000240.00412&radic S000001318VHR1YIL056WuncharacterizedTranscriptional activator, required for the vitaminH-responsive element (VHRE) mediated inductionof VHT1 (Vitamin H transporter) and BIO5(biotin biosynthesis intermediate transporter)in response to low biotin concentrations 0.000240.00412&radic S000006264ARO7YPR060CverifiedChorismate mutase, catalyzes the conversion of chorismate toprephenate to initiate thetyrosine/phenylalanine-specific branch ofaromatic amino acid biosynthesis 0.000240.00412&radic S000000912KAP123YER110CverifiedKaryopherin beta, mediates nuclear import of ribosomal proteinsprior to assembly into ribosomes and import ofhistones H3 and H4; localizes to the nuclearpore, nucleus, and cytoplasm; exhibits geneticinteractions with RAI1 0.000240.00412&radic S000003730CDC6YJL194WverifiedEssential ATP-binding protein required for DNA replication,component of the pre-replicative complex(pre-RC) which requires ORC to associate withchromatin and is in turn required for Mcm2-7pDNA association; homologous to S. pombe Cdc18p 0.000240.00412&radic S000003295SPT4YGR063CverifiedProtein that forms a complex with Spt5p and mediates bothactivation and inhibition of transcriptionelongation, and plays a role in pre-mRNAprocessing; in addition, Spt4p is involved inkinetochore function and gene silencing 0.000230.00406&radic S000005475PAP2YOL115WverifiedCatalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), anuclear poly (A) polymerase complex involved inRNA quality control; catalyzes polyadenylationof unmodified tRNAs, and snoRNA and rRNAprecursors; disputed role as a DNA polymerase 0.000230.00406&radic S000005732NOC2YOR206WverifiedProtein that forms a nucleolar complex with Mak21p that bindsto 90S and 66S pre-ribosomes, as well as anuclear complex with Noc3p that binds to 66Spre-ribosomes; both complexes mediateintranuclear transport of ribosomal precursors 0.000230.00406&radic S000002580ARG82YDR173CverifiedInositol polyphosphate multikinase (IPMK), sequentiallyphosphorylates Ins(1,4,5)P3 to formIns(1,3,4,5,6)P5; putative nuclear lipidinositol PI3-kinase; regulates arginine-,phosphate-, and nitrogen-responsive genes 0.000230.00406&radic S000001746KAE1YKR038CverifiedPutative glycoprotease proposed to be in transcription as acomponent of the EKC protein complex withBud32p, Cgi121p, Pcc1p, and Gon7p; alsoidentified as a component of the KEOPS proteincomplex 0.000230.00406&radic S000002412MAF1YDR005CverifiedNegative regulator of RNA polymerase III; component of severalsignaling pathways that repress polymerase IIItranscription in response to changes incellular environment; targets the initiationfactor TFIIIB 0.000230.00403&radic S000003125YGL157WVerifiedOxidoreductase, catalyzes NADPH-dependent reduction of the bicyclic diketone bicyclo[2.2.2]octane-2,6-dione (BCO2,6D) to the chiral ketoalcohol (1R,4S,6S)-6-hydroxybicyclo[2.2.2]octane-2-one (BCO2one6ol) 0.000230.00403&radic S000004256PDR8YLR266CverifiedTranscription factor; targets include ATP-binding cassette(ABC) transporters, major facilitatorsuperfamily transporters, and other genesinvolved in the pleiotropic drug resistance(PDR) phenomenon 0.000230.00396&radic S000001353YIL091CUncharacterizedProtein required for cell viability 0.000230.00396&radic S000005785RPT4YOR259CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for spindlepole body duplication; localized mainly to thenucleus throughout the cell cycle 0.000230.00396&radic S000000832CHZ1YER030WuncharacterizedPutative chaperone for Htz1p/H2A-H2B dimer 0.000230.00396&radic S000005195NRD1YNL251CverifiedRNA-binding protein that interacts with the C-terminal domainof the RNA polymerase II large subunit(Rpo21p), required for transcriptiontermination and 3' end maturation ofnonpolyadenylated RNAs 0.000230.00396&radic S000003957RIX7YLL034CverifiedPutative ATPase of the AAA family, required for export ofpre-ribosomal large subunits from the nucleus;distributed between the nucleolus, nucleoplasm,and nuclear periphery depending on growthconditions 0.000230.00396&radic&radic S000004871YMR258CUncharacterizedProtein of unknown function with similarity to F-box proteins; physically interacts with Skp1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR258C is not an essential gene 0.000230.00396&radic S000000804NOP16YER002WverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis 0.000230.00396&radic S000005411GAL11YOL051WverifiedComponent of the Mediator complex; interacts with RNApolymerase II and the general transcriptionfactors to form the RNA polymerase IIholoenzyme; affects transcription by acting astarget of activators and repressors 0.000230.00396&radic S000004791SPT21YMR179WverifiedProtein required for normal transcription at several lociincluding HTA2-HTB2 and HHF2-HHT2, but notrequired at the other histone loci;functionally related to Spt10p; involved intelomere maintenance 0.000230.00396&radic S000004034PDC1YLR044CverifiedMajor of three pyruvate decarboxylase isozymes, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde; subject to glucose-, ethanol-,and autoregulation; involved in amino acidcatabolism 0.000230.00396&radic S000001730NTR2YKR022CverifiedEssential protein that forms a dimer with Ntr1p; also forms atrimer, with Ntr2p and the DExD/H-box RNAhelicase Prp43p, that is involved inspliceosome disassembly 0.000230.00396&radic S000003901HIR3YJR140CverifiedTranscriptional corepressor involved in the cellcycle-regulated transcription of histone genesHTA1, HTB1, HHT1, and HHT2; involved inposition-dependent gene silencing andnucleosome reassembly 0.000230.00392&radic S000003563RNR2YJL026WverifiedRibonucleotide-diphosphate reductase (RNR), small subunit; theRNR complex catalyzes the rate-limiting step indNTP synthesis and is regulated by DNAreplication and DNA damage checkpoint pathwaysvia localization of the small subunits 0.000230.00392&radic S000005899NDD1YOR372CverifiedTranscriptional activator essential for nuclear division;localized to the nucleus; essential componentof the mechanism that activates the expressionof a set of late-S-phase-specific genes 0.000230.00392&radic S000000864HOR2YER062CverifiedOne of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1encodes the other) involved in glycerolbiosynthesis; induced in response tohyperosmotic stress and oxidative stress, andduring the diauxic transition 0.000230.00392&radic S000001022ECM29YHL030WverifiedMajor component of the proteasome; tethers the proteasome coreparticle to the regulatory particle, andenhances the stability of the proteasome 0.000230.00392&radic S000002738UBX5YDR330WuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p 0.000230.00385&radic S000000539LSB5YCL034WverifiedProtein of unknown function; binds Las17p, which is a homologof human Wiskott-Aldrich Syndrome proteininvolved in actin patch assembly and actinpolymerization 0.000230.00385&radic&radic S000004088CHA4YLR098CverifiedDNA binding transcriptional activator, mediatesserine/threonine activation of the catabolicL-serine (L-threonine) deaminase (CHA1);Zinc-finger protein with Zn[2]-Cys[6]fungal-type binuclear cluster domain 0.000230.00385&radic S000003026RAD6YGL058WverifiedUbiquitin-conjugating enzyme (E2), involved in postreplicationrepair (with Rad18p), sporulation, telomeresilencing, and ubiquitin-mediated N-end ruleprotein degradation (with Ubr1p) 0.000230.00385&radic&radic S000001672HYM1YKL189WverifiedComponent of the RAM signaling network that is involved inregulation of Ace2p activity and cellularmorphogenesis, interacts with Kic1p and Sog2p,localizes to sites of polarized growth duringbudding and during the mating response 0.000230.00385&radic S000006348NOC4YPR144CverifiedNucleolar protein, forms a complex with Nop14p that mediatesmaturation and nuclear export of 40S ribosomalsubunits 0.000230.00385&radic S000006347RRP15YPR143WverifiedNucleolar protein, constituent of pre-60S ribosomal particles;required for processing of the 27S pre-rRNA atthe A2 site to yield 5.8S and 25S rRNA 0.000230.00385&radic S000001668ASH1YKL185WverifiedZinc-finger inhibitor of HO transcription; mRNA is localizedand translated in the distal tip of anaphasecells, resulting in accumulation of Ash1p indaughter cell nuclei and inhibition of HOexpression; potential Cdc28p substrate 0.000230.00379&radic S000005098YCK2YNL154CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck1p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck1p 0.000230.00379&radic S000000132YBL036CVerifiedSingle-domain racemase, possibly non-specific due to the lack of the second domain, which presumably determines specificity 0.000230.00379&radic S000004076SMC4YLR086WverifiedSubunit of the condensin complex, which reorganizes chromosomesduring cell division, forms a stable complexwith Smc2p that has ATP-hydrolyzing andDNA-binding activity and promotes knotting ofcircular DNA; potential Cdc28p substrate 0.000230.00379&radic S000004443LEU3YLR451WverifiedZinc-finger transcription factor that regulates genes involvedin branched chain amino acid biosynthesis andammonia assimilation; positively regulated byalpha-isopropylmalate, an intermediate inleucine biosynthesis 0.000230.00379&radic S000003420BUB1YGR188CverifiedProtein kinase that forms a complex with Mad1p and Bub3p thatis crucial in the checkpoint mechanism requiredto prevent cell cycle progression into anaphasein the presence of spindle damage, associateswith centromere DNA via Skp1p 0.000230.00379&radic S000002163MED2YDL005CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation 0.000230.00379&radic&radic S000005996GCR1YPL075WverifiedTranscriptional activator of genes involved in glycolysis;DNA-binding protein that interacts andfunctions with the transcriptional activatorGcr2p 0.000230.00379&radic S000004117APC2YLR127CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; similar tocullin Cdc53p 0.000230.00379&radic S000001354YIL092WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus 0.000230.00379&radic S000005952PHO85YPL031CverifiedCyclin-dependent kinase, with ten cyclin partners; involved inenvironmental stress response; inphosphate-rich conditions, Pho85p-Pho80pcomplex phosphorylates Pho4p which in turnrepresses PHO5 0.000230.00379&radic S000005080EAF7YNL136WverifiedSubunit of the NuA4 histone acetyltransferase complex, whichacetylates the N-terminal tails of histones H4and H2A 0.000230.00376&radic S000000540GRX1YCL035CverifiedHydroperoxide and superoxide-radical responsive heat-stableglutathione-dependent disulfide oxidoreductasewith active site cysteine pair; protects cellsfrom oxidative damage 0.000230.00376&radic&radic S000004564TAF13YML098WverifiedTFIID subunit (19 kDa), involved in RNA polymerase IItranscription initiation, similar to histone H4with atypical histone fold motif of Spt3-liketranscription factors 0.000230.00376&radic S000002494RRP1YDR087CverifiedEssential evolutionarily conserved nucleolar protein necessaryfor biogenesis of 60S ribosomal subunits andprocessing of pre-rRNAs to mature rRNAs,associated with several distinct 66Spre-ribosomal particles 0.000230.00376&radic S000002411RAD57YDR004WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad55p 0.000230.00376&radic S000005603RTS2YOR077WverifiedBasic zinc-finger protein, similar to human and mouse Kin17proteins which are chromatin-associatedproteins involved in UV response and DNAreplication 0.000230.00376&radic S000006048HHO1YPL127CverifiedHistone H1, a linker histone required for nucleosome packagingat restricted sites; suppresses DNA repairinvolving homologous recombination; notrequired for telomeric silencing, basaltranscriptional repression, or efficientsporulation 0.000230.00376&radic S000003093MET13YGL125WverifiedIsozyme of methylenetetrahydrofolate reductase, catalyzes thereduction of 5,10-methylenetetrahydrofolate to5-methyltetrahydrofolate in the methioninebiosynthesis pathway 0.000230.00376&radic S000003858JJJ3YJR097WverifiedProtein of unknown function, contains a J-domain, which is aregion with homology to the E. coli DnaJprotein 0.000230.00376&radic S000001600SBA1YKL117WverifiedCo-chaperone that binds to and regulates Hsp90 familychaperones; important for pp60v-src activity inyeast; homologous to the mammalian p23 proteins 0.000230.00376&radic S000004244NDL1YLR254Cuncharacterizedhomolog of nuclear distribution factor NudE, NUDEL 0.000230.0037&radic S000000401YBR197CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene 0.000230.0037&radic S000000362AMN1YBR158WverifiedProtein required for daughter cell separation, multiple mitoticcheckpoints, and chromosome stability; contains12 degenerate leucine-rich repeat motifs;expression is induced by the Mitotic ExitNetwork (MEN) 0.000230.0037&radic S000000949SCC4YER147CverifiedSubunit of cohesin loading factor (Scc2p-Scc4p), a complexrequired for the loading of cohesin complexesonto chromosomes; involved in establishingsister chromatid cohesion during double-strandbreak repair via phosphorylated histone H2AX 0.000230.0037&radic S000005230CUS2YNL286WverifiedProtein that binds to U2 snRNA and Prp11p, may be involved inU2 snRNA folding; contains two RNA recognitionmotifs (RRMs) 0.000230.0037&radic S000006365SGV1YPR161CverifiedCyclin (Bur2p)-dependent protein kinase that functions intranscriptional regulation; phosphorylates thecarboxy-terminal domain of Rpo21p, which is thelargest subunit of RNA polymerase II; regulatedby Cak1p 0.000230.0037&radic S000004745PSO2YMR137CverifiedRequired for a post-incision step in the repair of DNA singleand double-strand breaks that result frominterstrand crosslinks produced by a variety ofmono- and bi-functional psoralen derivatives;induced by UV-irradiation 0.000230.0037&radic S000003693FAR1YJL157CverifiedCyclin-dependent kinase inhibitor that mediates cell cyclearrest in response to pheromone; also forms acomplex with Cdc24p, Ste4p, and Ste18p that mayspecify the direction of polarized growthduring mating; potential Cdc28p substrate 0.000230.0037&radic S000006223CDC54YPR019WverifiedEssential helicase component of heterohexameric MCM2-7complexes which bind pre-replication complexeson DNA and melt the DNA prior to replication;accumulates in the nucleus in G1; homolog of S.pombe Cdc21p 0.000230.00363&radic S000005577YOR051CUncharacterizedNuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts 0.000230.00363&radic S000004651CSM3YMR048WverifiedProtein required for accurate chromosome segregation duringmeiosis 0.000230.00363&radic S000001366SHQ1YIL104CverifiedEssential nuclear protein, required for accumulation of boxH/ACA snoRNAs and for rRNA processing;interacts with Naf1p 0.000230.00363&radic S000002599HST4YDR191WverifiedMember of the Sir2 family of NAD(+)-dependent proteindeacetylases; involved along with Hst3p insilencing at telomeres, cell cycle progression,radiation resistance, genomic stability andshort-chain fatty acid metabolism 0.000230.00363&radic S000002394YPD1YDL235CverifiedPhosphorelay intermediate protein, phosphorylated by the plasmamembrane sensor Sln1p in response to osmoticstress and then in turn phosphorylates theresponse regulators Ssk1p in the cytosol andSkn7p in the nucleus 0.000230.00363&radic S000005420GPD2YOL059WverifiedNAD-dependent glycerol 3-phosphate dehydrogenase, homolog ofGpd1p, expression is controlled by anoxygen-independent signaling pathway requiredto regulate metabolism under anoxic conditions;located in cytosol and mitochondria 0.000230.00363&radic S000003039AFT1YGL071WverifiedTranscription factor involved in iron utilization andhomeostasis; binds the consensus sitePyPuCACCCPu and activates the expression oftarget genes in response to changes in ironavailability 0.000230.00363&radic S000003992NOC3YLR002CverifiedProtein that forms a nuclear complex with Noc2p that binds to66S ribosomal precursors to mediate theirintranuclear transport; also binds to chromatinto promote the association of DNA replicationfactors and replication initiation 0.000230.00363&radic S000004539FPR3YML074CverifiedNucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506binding protein; phosphorylated by caseinkinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) anddephosphorylated by Ptp1p 0.000230.00363&radic S000004410CDC73YLR418CverifiedConstituent of Paf1 complex with RNA polymerase II, Paf1p,Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinctfrom Srb-containing Pol II complexes; requiredfor expression of certain genes, modificationof some histones, and telomere maintenance 0.000230.00363&radic S000006250MCM16YPR046WverifiedProtein involved in kinetochore-microtubule mediated chromosomesegregation; binds to centromere DNA 0.000230.00363&radic S000004967YNL022CUncharacterizedPutative protein of unknown function with seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to a single spot in the nucleus; YNL022C is not an essential gene 0.000230.00363&radic S000003816HIT1YJR055WverifiedProtein of unknown function, required for growth at hightemperature 0.000230.00358&radic S000003646GZF3YJL110CverifiedGATA zinc finger protein and Dal80p homolog that negativelyregulates nitrogen catabolic gene expression bycompeting with Gat1p for GATA site binding;function requires a repressive carbon source;dimerizes with Dal80p and binds to Tor1p 0.000230.00358&radic&radic S000002315YDL156WUncharacterizedHypothetical protein 0.000230.00358&radic S000002816ADE8YDR408CverifiedPhosphoribosyl-glycinamide transformylase, catalyzes a step inthe 'de novo' purine nucleotide biosyntheticpathway 0.000230.00358&radic S000005180SIN4YNL236WverifiedSubunit of the Mediator complex; interacts with the RNApolymerase II holoenzyme to postively ornegatively regulate transcription; dispensiblefor basal transcription 0.000230.00358&radic S000000339CKS1YBR135WverifiedSubunit of the Cdc28 protein kinase, required for mitoticproteolysis, may also be involved in theproteolysis of the G1 cyclins 0.000230.00358&radic S000003842EAF6YJR082CuncharacterizedEsa1p-associated factor, subunit of the NuA4 acetyltransferasecomplex 0.000230.00358&radic S000005274RPD3YNL330CverifiedHistone deacetylase; regulates transcription and silencing 0.000230.00358&radic S000005649LEO1YOR123CverifiedComponent of the Paf1 complex, which associates with RNApolymerase II and is involved in histonemethylation 0.000230.00358&radic S000004255NEJ1YLR265CverifiedProtein involved in regulation of nonhomologous end joining;repressed by MAT heterozygosity; associateswith Lif1p and regulates its cellulardistribution 0.000230.00358&radic&radic S000006114RSA1YPL193WverifiedProtein involved in the assembly of 60S ribosomal subunits;functionally interacts with Dbp6p; functions ina late nucleoplasmic step of the assembly 0.000230.00358&radic S000004048SHM2YLR058CverifiedCytosolic serine hydroxymethyltransferase, involved inone-carbon metabolism 0.000230.00358&radic&radic S000004990YNL045WVerifiedLeucyl aminopeptidase (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; role in vivo is not defined; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.000220.00356&radic S000003500HUA1YGR268CuncharacterizedCytoplasmic protein containing a zinc finger domain withsequence similarity to that of Type IJ-proteins; computational analysis oflarge-scale protein-protein interaction datasuggests a possible role in actin patchassembly 0.000220.00356&radic S000005165POP1YNL221CverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends; binds to the RPR1 RNAsubunit in Rnase P 0.000220.00356&radic S000002848DOT1YDR440WverifiedNucleosomal histone H3-Lys79 methylase, associates withtranscriptionally active genes, functions ingene silencing at telomeres, most likely bydirectly modulating chromatin structure and Sirprotein localization 0.000220.00356&radic S000005366TOP1YOL006CverifiedTopoisomerase I, nuclear enzyme that relieves torsional strainin DNA by cleaving and re-sealing thephosphodiester backbone; relaxes bothpositively and negatively supercoiled DNA;functions in replication, transcription, andrecombination 0.000220.00356&radic&radic S000002459DBF4YDR052CverifiedRegulatory subunit of Cdc7p-Dbf4p kinase complex, required forCdc7p kinase activity and initiation of DNAreplication; phosphorylates the Mcm2-7 familyof proteins; cell cycle regulated 0.000220.00356&radic S000001116QNS1YHR074WverifiedGlutamine-dependent NAD(+) synthetase, essential for theformation of NAD(+) from nicotinic acid adeninedinucleotide 0.000220.00356&radic S000005554CIN5YOR028CverifiedBasic leucine zipper transcriptional factor of the yAP-1 familythat mediates pleiotropic drug resistance andsalt tolerance; localizes constitutively to thenucleus 0.000220.00356&radic S000005964NOP4YPL043WverifiedNucleolar protein, essential for processing and maturation of27S pre-rRNA and large ribosomal subunitbiogenesis; constituent of 66S pre-ribosomalparticles; contains four RNA recognition motifs(RRMs) 0.000220.00356&radic S000001895DEG1YFL001WverifiedNon-essential tRNA:pseudouridine synthase, introducespseudouridines at position 38 or 39 in tRNA,important for maintenance of translationefficiency and normal cell growth, localizes toboth the nucleus and cytoplasm 0.000220.00356&radic S000005968SGF11YPL047WverifiedIntegral subunit of SAGA histone acetyltransferase complex,regulates transcription of a subset ofSAGA-regulated genes, required for the Ubp8passociation with SAGA and for H2Bdeubiquitylation 0.000220.00348&radic S000002298SCM3YDL139CuncharacterizedProtein of unknown function; isolated as a high-copy suppressorof mutations in the histone fold domain (HFD)of CSE4 0.000220.00348&radic S000002931SPS1YDR523CverifiedPutative protein serine/threonine kinase expressed at the endof meiosis and localized to the prosporemembrane, required for correct localization ofenzymes involved in spore wall synthesis 0.000220.00348&radic S000006140PCL8YPL219WverifiedCyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) tophosphorylate and regulate glycogen synthase,also activates Pho85p for Glc8p phosphorylation 0.000220.00348&radic S000002869MFA1YDR461WverifiedMating pheromone a-factor, made by a cells; interacts withalpha cells to induce cell cycle arrest andother responses leading to mating; biogenesisinvolves C-terminal modification, N-terminalproteolysis, and export; also encoded by MFA2 0.000220.00348&radic S000005959MET31YPL038WverifiedZinc-finger DNA-binding protein, involved in regulatingexpression of the methionine biosyntheticgenes, similar to Met32p 0.000220.00348&radic S000003849BIR1YJR089WverifiedEssential chromosomal passenger protein involved incoordinating cell cycle events for properchromosome segregation; C-terminal region bindsSli15p, and the middle region, uponphosphorylation, localizes Cbf2p to the spindleat anaphase 0.000220.00341&radic S000002589SAS4YDR181CverifiedSubunit of the SAS complex (Sas2p, Sas4p, Sas5p), whichacetylates free histones and nucleosomes andregulates transcriptional silencing; requiredfor the HAT activity of Sas2p 0.000220.00341&radic S000005989YPL068CUncharacterizedHypothetical protein 0.000220.00341&radic S000004960PBI2YNL015WverifiedCytosolic inhibitor of vacuolar proteinase B, required forefficient vacuole inheritance; with thioredoxinforms protein complex LMA1, which assists inpriming SNARE molecules and promotes vacuolefusion 0.000220.00341&radic S000002981PDR1YGL013CverifiedZinc cluster protein that is a master regulator involved inrecruiting other zinc cluster proteins topleiotropic drug response elements (PDREs) tofine tune the regulation of multidrugresistance genes 0.000220.00341&radic S000004640MSN2YMR037CverifiedTranscriptional activator related to Msn4p; activated in stressconditions, which results in translocation fromthe cytoplasm to the nucleus; binds DNA atstress response elements of responsive genes,inducing gene expression 0.000220.00341&radic S000000735GCN4YEL009CverifiedTranscriptional activator of amino acid biosynthetic genes inresponse to amino acid starvation; expressionis tightly regulated at both thetranscriptional and translational levels 0.000220.00341&radic S000002948IRC4YDR540CuncharacterizedHypothetical protein; null mutant displays increased levels ofspontaneous Rad52 foci 0.000220.00341&radic S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 0.000220.00341&radic S000000150YBL054WUncharacterizedHypothetical protein 0.000220.00341&radic S000003177MIG2YGL209WverifiedProtein containing zinc fingers, involved in repression, alongwith Mig1p, of SUC2 (invertase) expression byhigh levels of glucose; binds to Mig1p-bindingsites in SUC2 promoter 0.000220.00341&radic S000003443ZPR1YGR211WverifiedEssential protein with two zinc fingers, present in the nucleusof growing cells but relocates to the cytoplasmin starved cells via a process mediated byCpr1p; binds to translation elongation factoreEF-1 (Tef1p) 0.000220.00341&radic S000000965YER163CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.000220.00341&radic S000002928YDR520CUncharacterizedHypothetical protein 0.000220.00341&radic S000004281YLR290CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR290C is not an essential gene 0.000220.00341 S000002395PHO13YDL236WverifiedAlkaline phosphatase specific for p-nitrophenyl phosphate,involved in dephosphorylation of histone II-Aand casein 0.000220.00341&radic&radic S000000605PGK1YCR012Wverified3-phosphoglycerate kinase, catalyzes transfer of high-energyphosphoryl groups from the acyl phosphate of1,3-bisphosphoglycerate to ADP to produce ATP;key enzyme in glycolysis and gluconeogenesis 0.000220.00341&radic S000002222UBC9YDL064WverifiedSUMO-conjugating enzyme involved in the Smt3p conjugationpathway; nuclear protein required for S- andM-phase cyclin degradation and mitotic control;involved in proteolysis mediated by theanaphase-promoting complex cyclosome (APCC) 0.000220.00341&radic S000000215IPP1YBR011CverifiedCytoplasmic inorganic pyrophosphatase (PPase), catalyzes therapid exchange of oxygens from Pi with water,highly expressed and essential for viability,active-site residues show identity to thosefrom E. coli PPase 0.000220.00341&radic S000003769TDH2YJR009CverifiedGlyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involvedin glycolysis and gluconeogenesis; tetramerthat catalyzes the reaction ofglyceraldehyde-3-phosphate to 1,3bis-phosphoglycerate; detected in the cytoplasmand cell-wall 0.000220.00341&radic S000003099SNT2YGL131CverifiedDNA binding protein with similarity to the S. pombe Snt2protein 0.000220.00341&radic S000000114POP8YBL018CverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.000220.00341&radic S000000398SOY1YBR194WuncharacterizedProtein proposed to be associated with the nuclear pore complex 0.000220.00341&radic S000003262POP6YGR030CverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.000220.00341&radic S000000311IML3YBR107CverifiedProtein with a role in kinetochore function, localizes to theouter kinetochore in a Ctf19p-dependent manner,interacts with Chl4p and Ctf19p 0.000220.00341&radic S000000277RDH54YBR073WverifiedDNA-dependent ATPase, stimulates strand exchange by modifyingthe topology of double-stranded DNA; involvedin the recombinational repair of double-strandbreaks in DNA during mitosis and meiosis;proposed to be involved in crossoverinterference 0.000220.00341&radic S000004565ARG81YML099CverifiedZinc-finger transcription factor of the Zn(2)-Cys(6) binuclearcluster domain type, involved in the regulationof arginine-responsive genes; acts with Arg80pand Arg82p 0.000220.00341&radic S000004466YAP1YML007WverifiedBasic leucine zipper (bZIP) transcription factor required foroxidative stress tolerance; mediatespleiotropic drug and metal resistance;localized to the nucleus in response to thepresence of oxidants 0.000220.00341&radic S000004108YLR118CVerifiedAcyl-protein thioesterase responsible for depalmitoylation of Gpa1p; the mutant has no gross defects in signaling through the mating pathway; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.000220.00341&radic S000001726YKR018CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.000220.00341&radic S000004349RSC2YLR357WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; involvedin telomere maintenance 0.000220.00341&radic S000001363XBP1YIL101CverifiedTranscriptional repressor that binds to promoter sequences ofthe cyclin genes, CYS3, and SMF2; expression isinduced by stress or starvation during mitosis,and late in meiosis; member of the Swi4p/Mbp1pfamily; potential Cdc28p substrate 0.000220.00341&radic S000005099YNL155WUncharacterizedHypothetical protein 0.000220.00341&radic S000004646MCM1YMR043WverifiedTranscription factor involved in cell-type-specifictranscription and pheromone response; plays acentral role in the formation of both repressorand activator complexes 0.000220.00341&radic S000002776YPR1YDR368Wverified2-methylbutyraldehyde reductase, may be involved in isoleucinecatabolism 0.000220.00341&radic&radic S000003457AMA1YGR225WverifiedActivator of meiotic anaphase promoting complex (APC/C); Cdc20pfamily member; required for initiation of sporewall assembly; required for Clb1p degradationduring meiosis 0.000220.00341 S000004292YLR301WUncharacterizedProtein of unknown function that interacts with Sec72p 0.000220.00341&radic S000005012FKH2YNL068CverifiedForkhead family transcription factor with a major role in theexpression of G2/M phase genes; positivelyregulates transcriptional elongation; negativerole in chromatin silencing at HML and HMR;substrate of the Cdc28p/Clb5p kinase 0.000220.00341&radic S000006226YPR022CUncharacterizedHypothetical protein 0.000220.00341&radic S000000392NTC20YBR188CverifiedMember of a complex, including Prp19p, that binds to thespliceosome; required for pre-mRNA splicing 0.000220.00341&radic S000002862GUK1YDR454CverifiedGuanylate kinase, converts GMP to GDP; required for growth andmannose outer chain elongation of cell wallN-linked glycoproteins 0.000220.00341&radic S000006356URN1YPR152CuncharacterizedPre-mRNA splicing factor associated with the U2-U5-U6 snRNPs,the RES complex, and the Prp19-associatedcomplex (NTC); null mutation displays syntheticgenetic interactions with mutations affectingU2 snRNA and pre-mRNA splicing factors 0.000220.00341&radic S000002530INO2YDR123CverifiedComponent of the heteromeric Ino2p/Ino4p basic helix-loop-helixtranscription activator that bindsinositol/choline-responsive elements (ICREs),required for derepression of phospholipidbiosynthetic genes in response to inositoldepletion 0.000220.00341&radic S000006339CTF4YPR135WverifiedChromatin-associated protein, required for sister chromatidcohesion; interacts with DNA polymerase alpha(Pol1p) and may link DNA synthesis to sisterchromatid cohesion 0.000220.00341&radic S000005390GAS5YOL030WverifiedPutative 1,3-beta-glucanosyltransferase, has similarity toGas1p; localizes to the cell wall 0.000220.00338&radic S000005226POP3YNL282WverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.000220.00338&radic S000002269RRP42YDL111CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp4p, Rrp41p,Rrp43p and Dis3p 0.000220.00338&radic S000002977LEU1YGL009CverifiedIsopropylmalate isomerase, catalyzes the second step in theleucine biosynthesis pathway 0.000220.00338&radic S000006129RKM1YPL208WverifiedSET-domain lysine-N-methyltransferase, catalyzes the formationof dimethyllysine residues on the largeribsomal subunit protein L23a (RPL23A andRPL23B) 0.000220.00338&radic S000005218YNL274CUncharacterizedPutative hydroxyisocaproate dehydrogenase 0.000220.00338&radic S000004847RNH1YMR234WverifiedRibonuclease H1, removes RNA primers during Okazaki fragmentsynthesis; degrades RNA attached to the 5'-endof a DNA strand 0.000220.00338&radic S000002871STP1YDR463WverifiedTranscription factor, activated by proteolytic processing inresponse to signals from the SPS sensor systemfor external amino acids; activatestranscription of amino acid permease genes andmay have a role in tRNA processing 0.000220.00338&radic S000001470DAL7YIR031CverifiedMalate synthase, role in allantoin degradation unknown;expression sensitive to nitrogen cataboliterepression and induced by allophanate, anintermediate in allantoin degradation 0.000220.00338&radic S000001372MNI1YIL110WuncharacterizedPutative S-adenosylmethionine-dependent methyltransferase ofthe seven beta-strand family; deletion mutantexhibits a weak vacuolar protein sortingdefect, enhanced resistance to caspofungin, andis synthetically lethal with MEN mutants 0.000220.00338&radic S000003507RTT102YGR275WverifiedComponent of both the SWI/SNF and RSC chromatin remodelingcomplexes, suggested role in chromosomemaintenance; possible weak regulator of Ty1transposition 0.000220.00338&radic&radic S000003592ZAP1YJL056CverifiedZinc-regulated transcription factor, binds to zinc-responsivepromoter elements to induce transcription ofcertain genes in the presence of zinc;regulates its own transcription; contains sevenzinc-finger domains 0.000220.00338&radic S000000183RPL23AYBL087CverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl23Bp and has similarity to E.coli L14 and rat L23 ribosomal proteins 0.000220.00338&radic S000001521RGT1YKL038WverifiedGlucose-responsive transcription factor that regulatesexpression of several glucose transporter (HXT)genes in response to glucose; binds topromoters and acts both as a transcriptionalactivator and repressor 0.000220.00338&radic S000004470SPT5YML010WverifiedProtein that forms a complex with Spt4p and mediates bothactivation and inhibition of transcriptionelongation; Spt4p-Spt5p complex also plays arole in pre-mRNA processing 0.000220.00338&radic S000000009SWC3YAL011WverifiedProtein of unknown function, component of the SWR1 complex,which exchanges histone variant H2AZ (Htz1p)for chromatin-bound histone H2A; required forformation of nuclear-associated array of smoothendoplasmic reticulum known as karmellae 0.000220.00338&radic S000001244PPX1YHR201CverifiedExopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P)into Pi residues; located in the cytosol,plasma membrane, and mitochondrial matrix 0.000220.00338&radic S000005068NAF1YNL124WverifiedProtein required for the assembly of box H/ACA snoRNPs and forpre-rRNA processing, forms a complex with Shq1pand interacts with H/ACA snoRNP componentsNhp2p and Cbf5p; has similarity to Gar1p andother RNA-binding proteins 0.000220.00338&radic S000002696NSE3YDR288WuncharacterizedEssential subunit of the Mms21-Smc5-Smc6 complex; protein ofunknown function; required for DNA repair andgrowth 0.000220.00331&radic&radic S000002287YDL129WUncharacterizedHypothetical protein 0.000220.00331&radic S000005873REV1YOR346WverifiedDeoxycytidyl transferase, forms a complex with the subunits ofDNA polymerase zeta, Rev3p and Rev7p; involvedin repair of abasic sites in damaged DNA 0.000220.00331&radic&radic S000000656TAH1YCR060WverifiedHSP90 cofactor; interacts with Hsp82p, Pih1p, Rvb1 and Rvb2,contains a single TPR domain with at least twoTPR motifs 0.000220.00331&radic S000002568YDR161WUncharacterizedinteracts with PP2C 0.000220.00331&radic S000004937ADH6YMR318CverifiedNADPH-dependent cinnamyl alcohol dehydrogenase family memberwith broad substrate specificity; may beinvolved in fusel alcohol synthesis or inaldehyde tolerance 0.000220.00331&radic S000004570CAC2YML102WverifiedComponent of the chromatin assembly complex (with Rlf2p andMsi1p) that assembles newly synthesizedhistones onto recently replicated DNA, requiredfor building functional kinetochores, conservedfrom yeast to humans 0.000220.00331&radic S000004629YMR027WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene 0.000220.00331&radic S000000294YBR090CUncharacterizedHypothetical protein 0.000220.00331&radic&radic S000000891PTC2YER089CverifiedType 2C protein phosphatase; dephosphorylates Hog1p to limitmaximal osmostress induced kinase activity;dephosphorylates Ire1p to downregulate theunfolded protein response; dephosphorylatesCdc28p; role in DNA checkpoint inactivation 0.000220.00331&radic S000004949HRB1YNL004WverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toGbp2p and Npl3p 0.000220.00331&radic S000004890FCP1YMR277WverifiedCarboxy-terminal domain (CTD) phosphatase, essential fordephosphorylation of the repeated C-terminaldomain of the RNA polymerase II large subunit(Rpo21p) 0.000220.00331&radic S000001647PIR1YKL164CverifiedO-glycosylated protein required for cell wall stability;attached to the cell wall via beta-1,3-glucan;mediates mitochondrial translocation of Apn1p;expression regulated by the cell integritypathway and by Swi5p during the cell cycle 0.000220.00331&radic S000000536RRP7YCL031CverifiedEssential protein involved in rRNA processing and ribosomebiogenesis 0.000220.00331&radic S000006149CET1YPL228WverifiedBeta (RNA 5'-triphosphatase) subunit of the mRNA cappingenzyme, a heterodimer (the other subunit isCEG1, a guanylyltransferase) involved in addingthe 5' cap to mRNA; the mammalian enzyme is asingle bifunctional polypeptide 0.000220.00331&radic S000001177YCK1YHR135CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck2p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck2p 0.000220.00331&radic S000000609YCR016WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; YCR016W is not an essential gene 0.000220.00331&radic S000004370SEC61YLR378CverifiedEssential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p);forms a channel for SRP-dependent proteinimport and retrograde transport of misfoldedproteins out of the ER; with Sec63 complexallows SRP-independent protein import into ER 0.000220.00331&radic S000001451SQT1YIR012WverifiedEssential protein involved in a late step of 60S ribosomalsubunit assembly or modification; containsmultiple WD repeats; interacts with Qsr1p in atwo-hybrid assay 0.000220.00331&radic S000004134ACF2YLR144CverifiedIntracellular beta-1,3-endoglucanase, expression is inducedduring sporulation; may have a role in corticalactin cytoskeleton assembly 0.000220.00331&radic S000000483PAF1YBR279WverifiedRNA polymerase II-associated protein, defines a large complexthat is biochemically and functionally distinctfrom the Srb-Mediator form of Pol II holoenzymeand is required for full expression of a subsetof cell cycle-regulated genes 0.000220.00331&radic S000006331YPR127WUncharacterizedHypothetical protein 0.000220.00331&radic S000004445RIF2YLR453CverifiedProtein that binds to the Rap1p C-terminus and actssynergistically with Rif1p to help controltelomere length and establish telomericsilencing; deletion results in telomereelongation 0.000220.00331&radic S000001505CDC16YKL022CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; required forsporulation 0.000220.00331&radic S000001608RRN3YKL125WverifiedProtein required for transcription of rDNA by RNA polymerase I;transcription factor independent of DNAtemplate; involved in recruitment of RNApolymerase I to rDNA 0.000220.00331&radic&radic S000004557PRE8YML092Cverified20S proteasome beta-type subunit 0.000220.00331&radic S000003223FZF1YGL254WverifiedTranscription factor involved in sulfite metabolism, soleidentified regulatory target is SSU1,overexpression suppresses sulfite-sensitivityof many unrelated mutants due tohyperactivation of SSU1, contains five zincfingers 0.000220.00331&radic S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.000220.00328&radic&radic S000002922YDR514CUncharacterizedHypothetical protein 0.000220.00328&radic S000002994TRP5YGL026CverifiedTryptophan synthase involved in tryptophan biosynthesis,regulated by the general control system ofamino acid biosynthesis 0.000220.00328&radic S000003925RTT109YLL002WverifiedProtein with a role in regulation of Ty1 transposition 0.000220.00328&radic S000002697RTT103YDR289CverifiedProtein that interacts with exonuclease Rat1p and Rai1p andplays a role in transcription termination byRNA polymerase II, has an RPR domain(carboxy-terminal domain interacting domain);also involved in regulation of Ty1transposition 0.000220.00328 S000004433RPS1AYLR441CverifiedRibosomal protein 10 (rp10) of the small (40S) subunit; nearlyidentical to Rps1Bp and has similarity to ratS3a ribosomal protein 0.000220.00328&radic S000000199RTG3YBL103CverifiedBasic helix-loop-helix-leucine zipper (bHLH/Zip) transcriptionfactor that forms a complex with anotherbHLH/Zip protein, Rtg1p, to activate theretrograde (RTG) and TOR pathways 0.000220.00328&radic S000003361SYF2YGR129WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression 0.000220.00328&radic S000005574RAT1YOR048CverifiedNuclear 5' to 3' single-stranded RNA exonuclease, involved inRNA metabolism, including rRNA and snRNAprocessing as well as mRNA transcriptiontermination 0.000220.00328&radic S000003219YGL250WUncharacterizedPutative protein of unknown function; deletion mutant results in reduced PIS1 expression and has growth defects on non-fermentable carbon sources and on minimal media; GFP-fusion protein localizes to both the cytoplasm and the nucleus 0.000220.00328&radic S000003628HPR5YJL092WverifiedDNA helicase and DNA-dependent ATPase involved in DNA repair,required for proper timing of commitment tomeiotic recombination and the transition fromMeiosis I to Meiosis II; potential Cdc28psubstrate 0.000220.00328&radic S000005315PPG1YNR032WverifiedPutative serine/threonine protein phosphatase, required forglycogen accumulation; interacts with Tap42p,which binds to and regulates other proteinphosphatases 0.000220.00328&radic S000001666LOT5YKL183WverifiedProtein of unknown function; gene expression increases incultures shifted to a lower temperature 0.000220.00328&radic S000002301CRD1YDL142CverifiedCardiolipin synthase; produces cardiolipin, which is animportant constituent of mitochondrialmembranes; required for normal mitochondrialmembrane potential and function 0.000220.00328 S000005700MED4YOR174WverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation 0.000220.00328&radic S000001613SHE2YKL130CverifiedRNA-binding protein that binds specific mRNAs and interactswith She3p; part of the mRNA localizationmachinery that restricts accumulation ofcertain proteins to the bud 0.000220.00328&radic S000005648PFY1YOR122CverifiedProfilin, actin- and phosphatidylinositol4,5-bisphosphate-binding protein, plays a rolein cytoskeleton organization, required fornormal timing of actin polymerization inresponse to thermal stress; localizes to plasmamembrane and cytosol 0.000220.00328&radic S000003410PBP1YGR178CverifiedProtein interacting with poly(A)-binding protein Pab1p; likelyinvolved in controlling the extent of mRNApolyadenylation; forms a complex with Mkt1pthat may regulate HO translation; interactswith Lsm12p in a copurification assay 0.000220.00328&radic S000004147ASP3-2YLR157CverifiedCell-wall L-asparaginase II, involved in asparagine catabolism;expression is induced during nitrogenstarvation; four copies of ASP3 are present inthe genome reference strain S288C 0.000220.00328 S000006313YPR109WUncharacterizedHypothetical protein 0.000220.00328 S000004674MOT3YMR070WverifiedNuclear transcription factor with two Cys2-His2 zinc fingers;involved in repression of a subset of hypoxicgenes by Rox1p, repression of several DAN/TIRgenes during aerobic growth, and repression ofergosterol biosynthetic genes 0.000220.00328&radic S000003023OLE1YGL055WverifiedFatty acid desaturase, required for monounsaturated fatty acidsynthesis and for normal distribution ofmitochondria 0.000220.00328 S000003661GCD14YJL125CverifiedSubunit of tRNA (1-methyladenosine) methyltransferase, withGcd10p, required for the modification of theadenine at position 58 in tRNAs, especiallytRNAi-Met; first identified as a negativeregulator of GCN4 expression 0.000220.00328&radic&radic S000002711RSC3YDR303CverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; essential gene required forregulation of ribosomal protein genes and thecell wall/stress response; highly similar toRsc30p 0.000220.00328&radic S000005495MED7YOL135CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation 0.000220.00328&radic S000004033TRX1YLR043CverifiedCytoplasmic thioredoxin isoenzyme of the thioredoxin systemwhich protects cells against both oxidative andreductive stress, forms LMA1 complex withPbi2p, acts as a cofactor for Tsa1p, requiredfor ER-Golgi transport and vacuole inheritance 0.000220.00328&radic S000006124TPK2YPL203WverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; activates filamentous growth 0.000220.00328&radic S000004332RPP0YLR340WverifiedConserved ribosomal protein P0 similar to rat P0, human P0, andE. coli L10e; shown to be phosphorylated onserine 302 0.000220.00328 S000002814PDR15YDR406WverifiedATP binding cassette (ABC) transporter of the plasma membrane;general stress response factor implicated incellular detoxification; target of Pdr1p, Pdr3pand Pdr8p transcription regulators; promotercontains a PDR responsive element 0.000220.00328 S000000041PTA1YAL043CverifiedSubunit of holo-CPF, a multiprotein complex and functionalhomolog of mammalian CPSF, required for thecleavage and polyadenylation of mRNA and snoRNA3' ends; involved in pre-tRNA processing; bindsto the phosphorylated CTD of RNAPII 0.000220.00328&radic S000005111SKO1YNL167CverifiedBasic leucine zipper (bZIP) transcription factor of theATF/CREB family, forms a complex with Tup1p andSsn6p to both activate and represstranscription; cytosolic and nuclear proteininvolved in osmotic and oxidative stressresponses 0.000220.00328&radic S000000180CDC27YBL084CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition 0.000220.00328&radic S000005819RPS10AYOR293WverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps10Bp and has similarity to ratribosomal protein S10 0.000220.00328&radic S000002997CGR1YGL029WverifiedProtein involved in nucleolar integrity and processing of thepre-rRNA for the 60S ribosome subunit;transcript is induced in response to cytotoxicstress but not genotoxic stress 0.000220.00328&radic S000001051YHR009CUncharacterizedPutative protein of unknown function; not an essential gene 0.000220.00328&radic&radic S000001281FAF1YIL019WverifiedProtein required for pre-rRNA processing and 40S ribosomalsubunit assembly 0.000220.00328&radic S000004006PML1YLR016CverifiedSubunit of the RES complex, which is required for nuclearretention of unspliced pre-mRNAs; acts in thesame pathway as Pml39p and Mlp1p 0.000220.00324&radic S000002803SXM1YDR395WverifiedNuclear transport factor (karyopherin) involved in proteintransport between the cytoplasm andnucleoplasm; similar to Nmd5p, Cse1p, Lph2p,and the human cellular apoptosis susceptibilityprotein, CAS1 0.000220.00324&radic S000002562CPR1YDR155CverifiedCytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin),catalyzes the cis-trans isomerization ofpeptide bonds N-terminal to proline residues;binds the drug cyclosporin A 0.000220.00324&radic&radic S000002680GLO2YDR272WverifiedCytoplasmic glyoxalase II, catalyzes the hydrolysis ofS-D-lactoylglutathione into glutathione andD-lactate 0.000220.00324&radic S000002263NSE4YDL105WverifiedNuclear protein that plays a role in the function of theSmc5p-Rhc18p complex 0.000220.00324&radic S000002851SSN2YDR443CverifiedProtein required for stable association of Srb10p-Srb11p kinasewith RNA polymerase holoenzyme; subunit of theRNA polymerase II mediator complex; essentialfor transcriptional regulation 0.000220.00324&radic S000001676SDS22YKL193CverifiedConserved nuclear regulatory subunit of Glc7p type 1 proteinserine-threonine phosphatase (PP1), functionspositively with Glc7p to promotedephosphorylation of nuclear substratesrequired for chromosome transmission duringmitosis 0.000220.00324&radic S000003583RTT101YJL047CverifiedCullin subunit of a Roc1p-dependent E3 ubiquitin ligasecomplex; deletion phenotype suggests a role inanaphase progression; interacts with Mms22p andimplicated in Mms22-dependent DNA repair;modified by the ubiquitin-like protein, Rub1p 0.000220.00324&radic S000004928GLC8YMR311CverifiedRegulatory subunit of protein phosphatase 1 (Glc7p), involvedin glycogen metabolism and chromosomesegregation; proposed to regulate Glc7pactivity via conformational alteration;ortholog of the mammalian protein phosphataseinhibitor 2 0.000220.00324&radic S000000187MAP2YBL091CverifiedMethionine aminopeptidase, catalyzes the cotranslationalremoval of N-terminal methionine from nascentpolypeptides; function is partially redundantwith that of Map1p 0.000220.00324&radic S000002218TSR1YDL060WverifiedProtein required for processing of 20S pre-rRNA in thecytoplasm, associates with pre-40S ribosomalparticles 0.000220.00324&radic S000000643BUD23YCR047CverifiedProtein involved in bud-site selection; diploid mutants displaya random budding pattern instead of thewild-type bipolar pattern 0.000220.00324&radic S000007488YBL059C-AUncharacterizedHypothetical protein 0.000220.00324&radic&radic S000002299RPO21YDL140CverifiedRNA polymerase II largest subunit B220, part of central core;phosphorylation of C-terminal heptapeptiderepeat domain regulates association withtranscription and splicing factors; similar tobacterial beta-prime 0.000220.00324&radic S000000099HTA2YBL003CverifiedOne of two nearly identical (see also HTA1) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.000220.00324&radic S000001771LAS1YKR063CverifiedEssential nuclear protein possibly involved in bud formationand morphogenesis; mutants require the SSD1-vallele for viability 0.000220.00324&radic S000001597APN1YKL114CverifiedMajor apurinic/apyrimidinic endonuclease, 3'-repair diesteraseinvolved in repair of DNA damage by oxidationand alkylating agents; also functions as a3'-5' exonuclease to repair7,8-dihydro-8-oxodeoxyguanosine 0.000220.00324&radic S000004481OST6YML019WverifiedSubunit of the oligosaccharyltransferase complex of the ERlumen, which catalyzes asparagine-linkedglycosylation of newly synthesized proteins;similar to and partially functionally redundantwith Ost3p 0.000220.00324 S000001473LYS1YIR034CverifiedSaccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzesthe conversion of saccharopine to L-lysine,which is the final step in the lysinebiosynthesis pathway 0.000220.00324&radic S000003325YGR093WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus 0.000220.00324&radic S000002946PAD1YDR538WverifiedPhenylacrylic acid decarboxylase, confers resistance tocinnamic acid, decarboxylates aromaticcarboxylic acids to the corresponding vinylderivatives 0.000220.00324 S000001112TRM5YHR070WverifiedtRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacentto the anticodon that has a role in preventionof frameshifting; highly conserved acrossArchaea, Bacteria, and Eukarya 0.000220.00324&radic S000004494RAD52YML032CverifiedProtein that stimulates strand exchange by facilitating Rad51pbinding to single-stranded DNA; annealscomplementary single-stranded DNA; involved inthe repair of double-strand breaks in DNAduring vegetative growth and meiosis 0.000220.00324&radic S000002984KAP122YGL016WverifiedKaryopherin beta, responsible for import of the Toa1p-Toa2pcomplex into the nucleus; binds to nucleoporinsNup1p and Nup2p; may play a role in regulationof pleiotropic drug resistance 0.000220.00324&radic S000005116APC1YNL172WverifiedLargest subunit of the Anaphase-Promoting Complex/Cyclosome(APC/C), which is a ubiquitin-protein ligaserequired for degradation of anaphaseinhibitors, including mitotic cyclins, duringthe metaphase/anaphase transition 0.000220.00324&radic S000000165AST1YBL069WverifiedPeripheral membrane protein that interacts with the plasmamembrane ATPase Pma1p and has a role in itstargeting to the plasma membrane, possibly byinfluencing its incorporation into lipid rafts 0.000220.00324&radic&radic S000003011DST1YGL043WverifiedGeneral transcription elongation factor TFIIS, enables RNApolymerase II to read through blocks toelongation by stimulating cleavage of nascenttranscripts stalled at transcription arrestsites 0.000220.00324&radic S000004780ALD2YMR170CverifiedCytoplasmic aldehyde dehydrogenase, involved in ethanoloxidation and beta-alanine biosynthesis; usesNAD+ as the preferred coenzyme; expression isstress induced and glucose repressed; verysimilar to Ald3p 0.000220.00324&radic S000001532CSE4YKL049CverifiedCentromere protein that resembles histones, required for properkinetochore function; homolog of human CENP-A 0.000220.00324&radic S000003335NOP7YGR103WverifiedNucleolar protein involved in rRNA processing and 60S ribosomalsubunit biogenesis; constituent of severaldifferent pre-ribosomal particles; required forexit from G0 and the initiation ofcell proliferation 0.000220.00323&radic S000005363PFA4YOL003CverifiedPalmitoyltransferase with autoacylation activity, required forpalmitoylation of amino acid permeasescontaining a C-terminal Phe-Trp-Cys site;required for modification of Chs3p; member ofthe DHHC family of putativepalmitoyltransferases 0.000220.00323 S000002572TRM82YDR165WverifiedSubunit of a tRNA methyltransferase complex composed of Trm8pand Trm82p that catalyzes 7-methylguanosinemodification of tRNA 0.000220.00323&radic&radic S000004595ERG13YML126Cverified3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzesthe formation of HMG-CoA from acetyl-CoA andacetoacetyl-CoA; involved in the second step inmevalonate biosynthesis 0.000220.00323&radic S000003880JHD2YJR119CuncharacterizedPutative protein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmand nucleus 0.000220.00323&radic S000002506BMH2YDR099Wverified14-3-3 protein, minor isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.000220.00323&radic S000006379DPB2YPR175WverifiedSecond largest subunit of DNA polymerase II (DNA polymeraseepsilon), required for normal yeast chromosomalreplication; expression peaks at the G1/S phaseboundary; potential Cdc28p substrate 0.000220.00323&radic S000001240RIX1YHR197WverifiedEssential protein involved in the processing of the ITS2 regionof the rRNA locus; required for the maturationand nuclear export of the 60S ribosomal subunit 0.000220.00323&radic S000002545HPR1YDR138WverifiedSubunit of THO/TREX complexes that couple transcriptionelongation with mitotic recombination and withmRNA metabolism and export, subunit of an RNAPol II complex; regulates lifespan; involved intelomere maintenance; similar to Top1p 0.000220.00323&radic S000001504MAK11YKL021CverifiedProtein essential for cell growth and replication of M dsRNAvirus; contains four beta-transducin repeats 0.000220.00323&radic S000004534POB3YML069WverifiedSubunit of the heterodimeric FACT complex (Spt16p-Pob3p), whichfacilitates RNA Polymerase II transcriptionelongation through nucleosomes by destabilizingand then reassembling nucleosome structure 0.000220.00323&radic S000000649THR4YCR053WverifiedThreonine synthase, conserved protein that catalyzes formationof threonine from 0-phosphohomoserine;expression is regulated by the GCN4-mediatedgeneral amino acid control pathway 0.000220.00323&radic S000004665RNA14YMR061WverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; bridges interaction between Rna15p andHrp1p in the CF I complex 0.000220.00323&radic S000028548YGL041C-BUncharacterizedIdentified by fungal homology and RT-PCR 0.000220.00323 S000004224TOP3YLR234WverifiedDNA Topoisomerase III, conserved protein that functions in acomplex with Sgs1p and Rmi1p to relaxsingle-stranded negatively-supercoiled DNApreferentially, involved in telomere stabilityand regulation of mitotic recombination 0.000220.00323&radic S000001870EPL1YFL024CverifiedComponent of NuA4, which is an essential histone H4/H2Aacetyltransferase complex; homologous toDrosophila Enhancer of Polycomb 0.000220.00323&radic&radic S000000748GEA2YEL022WverifiedGuanine nucleotide exchange factor for ADP ribosylation factors(ARFs), involved in vesicular transport betweenthe Golgi and ER, Golgi organization, and actincytoskeleton organization; similar to but notfunctionally redundant with Gea1p 0.000220.00323 S000001849SEC53YFL045CverifiedPhosphomannomutase, involved in synthesis of GDP-mannose anddolichol-phosphate-mannose; required forfolding and glycosylation of secretory proteinsin the ER lumen 0.000220.00323&radic S000001493UFD4YKL010CverifiedUbiquitin-protein ligase (E3) that interacts with Rpt4p andRpt6p, two subunits of the 19S particle of the26S proteasome; cytoplasmic E3 involved in thedegradation of ubiquitin fusion proteins 0.000220.00323&radic S000001900RPN11YFR004WverifiedMetalloprotease subunit of the 19S regulatory particle of the26S proteasome lid; couples thedeubiquitination and degradation of proteasomesubstrates 0.000220.00323&radic S000001104RPP1YHR062CverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.000220.00316&radic S000001132YNG2YHR090CverifiedSubunit of the NuA4 histone acetyltransferase complex thatacetylates histone H4 and H2A; has similarityto the human tumor suppressor ING1 0.000220.00316&radic&radic S000003830HAM1YJR069CverifiedConserved protein with deoxyribonucleoside triphosphatepyrophosphohydrolase activity, mediatesexclusion of noncanonical purines fromdeoxyribonucleoside triphosphate pools; mutantis sensitive to the base analog6-N-hydroxylaminopurine 0.000220.00316&radic S000005639AZF1YOR113WverifiedZinc-finger transcription factor, involved in induction of CLN3transcription in response to glucose; geneticand physical interactions indicate a possiblerole in mitochondrial transcription or genomemaintenance 0.000220.00316&radic S000002798UBA2YDR390CverifiedNuclear protein that acts as a heterodimer with Aos1p toactivate Smt3p (SUMO) before its conjugation toproteins (sumoylation), which may play a rolein protein targeting; essential for viability 0.000220.00316&radic S000005428RTG1YOL067CverifiedTranscription factor (bHLH) involved in interorganellecommunication between mitochondria,peroxisomes, and nucleus 0.000220.00316&radic S000005586YOR060CUncharacterizedProtein required for cell viability 0.000220.00316&radic S000004497SRC1YML034WverifiedProtein with a putative role in sister chromatid segregation,potentially phosphorylated by Cdc28p; greenfluorescent protein (GFP)-fusion proteinlocalizes to the nuclear periphery 0.000220.00316 S000002547MTQ2YDR140WuncharacterizedS-adenosylmethionine-dependent methyltransferase of the sevenbeta-strand family; methylates release factoreRF1 (Sup45p) in vitro; is not an essentialgene; similar to E.coli PrmC 0.000220.00316&radic S000003005PNC1YGL037CverifiedNicotinamidase that converts nicotinamide to nicotinic acid aspart of the NAD(+) salvage pathway, requiredfor life span extension by calorie restriction;PNC1 expression responds to all known stimulithat extend replicative life span 0.000220.00316 S000002234RXT3YDL076CuncharacterizedProtein of unknown function, may be involved in chromatinsilencing 0.000220.00316&radic S000001201KEL1YHR158CverifiedProtein required for proper cell fusion and cell morphology;functions in a complex with Kel2p to negativelyregulate mitotic exit, interacts with Tem1p andLte1p; localizes to regions of polarizedgrowth; potential Cdc28p substrate 0.000220.00316 S000004472RAD33YML011CuncharacterizedProtein involved in nucleotide excision repair; greenfluorescent protein (GFP)-fusion proteinlocalizes to the nucleus 0.000220.00316&radic S000005419ARG1YOL058WverifiedArginosuccinate synthetase, catalyzes the formation ofL-argininosuccinate from citrulline andL-aspartate in the arginine biosynthesispathway; potential Cdc28p substrate 0.000220.00316&radic S000002309RPC53YDL150WverifiedRNA polymerase III subunit C53 0.000220.00316&radic S000003274YGR042WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 0.000220.00316&radic&radic S000006141RPL1AYPL220WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl1Bpand has similarity to E. coli L1 and rat L10aribosomal proteins; rpl1a rpl1b double nullmutation is lethal 0.000220.00316 S000002349UFD2YDL190CverifiedUbiquitin chain assembly factor (E4) that cooperates with aubiquitin-activating enzyme (E1), aubiquitin-conjugating enzyme (E2), and aubiquitin protein ligase (E3) to conjugateubiquitin to substrates; also functions as an 0.000220.00316&radic S000005784HNT3YOR258WverifiedMember of the third branch of the histidine triad (HIT)superfamily of nucleotide-binding proteins;similar to Aprataxin, a Hint related proteinthat is mutated in individuals with ataxia withoculomotor apraxia 0.000220.00316&radic S000000958YER156CUncharacterizedHypothetical protein 0.000220.00316&radic&radic S000003340CLB1YGR108WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 0.000220.00316&radic S000005102PGA1YNL158WverifiedEssential protein required for maturation of Gas1p and Pho8p;green fluorescent protein (GFP)-fusion proteinlocalizes to the nuclear periphery; hassynthetic genetic interations with secretorypathway genes 0.000220.00316 S000002565HOM2YDR158WverifiedAspartic beta semi-aldehyde dehydrogenase, catalyzes the secondstep in the common pathway for methionine andthreonine biosynthesis; expression regulated byGcn4p and the general control of amino acidsynthesis 0.000220.00316&radic&radic S000002584UBC1YDR177WverifiedUbiquitin-conjugating enzyme that mediates selectivedegradation of short-lived and abnormalproteins; plays a role in vesicle biogenesisand ER-associated protein degradation (ERAD);component of the cellular stress response 0.000220.00316&radic S000001222OYE2YHR179WverifiedWidely conserved NADPH oxidoreductase containing flavinmononucleotide (FMN), homologous to Oye3p withslight differences in ligand binding andcatalytic properties; may be involved in sterolmetabolism 0.000220.00316&radic S000004857YMR244C-AUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR244C-A is not an essential gene 0.000220.00316&radic S000000773YEL047CVerifiedFumurate ReDuctase Soluble 0.000210.00314&radic S000004724YMR118CUncharacterizedProtein of unknown function with similarity to succinate dehydrogenase cytochrome b subunit; YMR118C is not an essential gene 0.000210.00314 S000003766HYS2YJR006WverifiedDNA polymerase III (delta) subunit, essential for cellviability; involved in DNA replication and DNArepair 0.000210.00314&radic S000003538PRE3YJL001Wverified20S proteasome beta-type subunit, responsible for cleavageafter acidic residues in peptides 0.000210.00314&radic&radic S000005992YPL071CUncharacterizedHypothetical protein 0.000210.00314&radic S000006111NAB3YPL190CverifiedSingle stranded DNA binding protein; acidic ribonucleoprotein;required for termination of non-poly(A)transcripts and efficient splicing; interactswith Nrd1p 0.000210.00314&radic S000001550YNK1YKL067WverifiedNucleoside diphosphate kinase, catalyzes the transfer of gammaphosphates from nucleoside triphosphates,usually ATP, to nucleoside diphosphates by amechanism that involves formation of anautophosphorylated enzyme intermediate 0.000210.00314&radic S000000861PCL6YER059WverifiedPho85p cyclin of the Pho80p subfamily; forms the major Glc8pkinase together with Pcl7p and Pho85p; involvedin the control of glycogen storage by Pho85p;stabilized by Elongin C binding 0.000210.00314&radic S000002214MBP1YDL056WverifiedTranscription factor involved in regulation of cell cycleprogression from G1 to S phase, forms a complexwith Swi6p that binds to MluI cell cycle boxregulatory element in promoters of DNAsynthesis genes 0.000210.00314&radic S000004320NMA1YLR328WverifiedNicotinic acid mononucleotide adenylyltransferase, involved inNAD(+) salvage pathway 0.000210.00314&radic S000005689DDP1YOR163WverifiedDiadenosine polyphosphate hydrolase, member of the MutT familyof nucleotide hydrolases with high specificityfor diadenosine hexa- and pentaphosphates,required for efficient hydrolysis ofdiphosphorylated inositol polyphosphates 0.000210.00314&radic&radic S000001880HSP12YFL014WverifiedPlasma membrane localized protein that protects membranes fromdesiccation; induced by heat shock, oxidativestress, osmostress, stationary phase entry,glucose depletion, oleate and alcohol;regulated by the HOG and Ras-Pka pathways 0.000210.00314&radic S000002597SLY1YDR189WverifiedHydrophilic protein involved in vesicle trafficking between theER and Golgi; SM (Sec1/Munc-18) family proteinthat binds the tSNARE Sed5p and stimulates itsassembly into a trans-SNARE membrane-proteincomplex 0.000210.00314&radic S000000250ZTA1YBR046CverifiedZeta-crystallin homolog, found in the cytoplasm and nucleus;has similarity to E. coli quinoneoxidoreductase and to human zeta-crystallin,which has quinone oxidoreductase activity 0.000210.00314&radic S000001272DOT5YIL010WverifiedNuclear thiol peroxidase which functions as analkyl-hydroperoxide reductase duringpost-diauxic growth 0.000210.00314&radic S000004098YLR108CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene 0.000210.00314&radic S000002288RPP1BYDL130WverifiedRibosomal protein P1 beta, component of the ribosomal stalk,which is involved in interaction oftranslational elongation factors with ribosome;accumulation is regulated by phosphorylationand interaction with the P2 stalk component 0.000210.00314 S000002347PPH22YDL188CverifiedCatalytic subunit of protein phosphatase 2A, functionallyredundant with Pph21p; methylated at Cterminus; forms alternate complexes withseveral regulatory subunits; involved in signaltransduction and regulation of mitosis 0.000210.00314&radic&radic S000000286UBC4YBR082CverifiedUbiquitin-conjugating enzyme that mediates degradation ofshort-lived and abnormal proteins; interactswith E3-CaM in ubiquitinating calmodulin;interacts with many SCF ubiquitin proteinligases; component of the cellular stressresponse 0.000210.00314&radic S000006059SPP1YPL138CverifiedSubunit of COMPASS (Set1C), a complex which methylates histoneH3 on lysine 4 and is required in telomerictranscriptional silencing; PHD finger domainprotein similar to human CGBP, an unmethylatedCpG binding protein 0.000210.00314&radic S000002438MIC14YDR031WverifiedMitochondrial intermembrane space cysteine motif protein of 14kDa 0.000210.00314&radic S000004905YMR291WUncharacterizedPutative kinase of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR291W is not an essential gene 0.000210.00314&radic S000004313SFH1YLR321CverifiedSubunit of the RSC chromatin remodeling complex required forkinetochore function in chromosome segregation;essential gene required for cell cycleprogression; phosphorylated in the G1 phase ofthe cell cycle; Snf5p paralog 0.000210.00314&radic S000005016RNH201YNL072WverifiedRibonuclease H2 catalytic subunit, removes RNA primers duringOkazaki fragment synthesis; cooperates withRad27p nuclease 0.000210.00314&radic S000006333SCD6YPR129WverifiedProtein containing an Lsm domain, may bind RNA and have a rolein RNA processing; overproduction suppresses anull mutation in CHC1, which encodes the heavychain of clathrin 0.000210.00314 S000002379CDC13YDL220CverifiedSingle stranded DNA-binding protein found at TG1-3 telomereG-tails; regulates telomere replication throughrecruitment of specific sub-complexes, but theessential function is telomere capping 0.000210.00314&radic S000005682NFI1YOR156CverifiedSUMO ligase, catalyzes the covalent attachment of SUMO (Smt3p)to proteins; involved in maintenance of propertelomere length 0.000210.00314&radic S000005007MTQ1YNL063WuncharacterizedS-adenosylmethionine-dependent Methyltransferase; methylatestranslational release factor Mrf1p; similar toE.coli PrmC; is not an essential gene 0.000210.00314 S000003079NSA1YGL111WverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis 0.000210.00314&radic S000004393DUS3YLR401CuncharacterizedDihydrouridine synthase, member of a widespread family ofconserved proteins including Smm1p, Dus1p, andDus4p; contains a consensus oleate responseelement (ORE) in its promoter region 0.000210.00314&radic S000005285ATO2YNR002CverifiedPutative transmembrane protein, involved in the export ofammonia, a starvation signal that promotes celldeath in the center of aging colonies; memberof the TC 9.B.33 YaaH family; homolog of Ady2pand Y. lipolytica Gpr1p 0.000210.00314 S000005188SUI1YNL244CverifiedTranslation initiation factor eIF1; component of a complexinvolved in recognition of the initiator codon;modulates translation accuracy at theinitiation phase 0.000210.00314 S000001103GIC1YHR061CverifiedProtein of unknown function involved in initiation of buddingand cellular polarization, interacts withCdc42p via the Cdc42/Rac-interactive binding(CRIB) domain 0.000210.00314&radic S000005879YOR352WUncharacterizedHypothetical protein 0.000210.00314&radic S000002441LYS14YDR034CverifiedTranscriptional activator involved in regulation of genes ofthe lysine biosynthesis pathway; requires2-aminoadipate semialdehyde as co-inducer 0.000210.00314&radic S000005365RPB11YOL005CverifiedRNA polymerase II subunit B12.5; part of central core; similarto Rpc19p and bacterial alpha subunit 0.000210.00314&radic&radic S000003828YAE1YJR067CverifiedEssential protein of unknown function 0.000210.00314&radic S000006043TFB2YPL122CverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, involved in transcriptioninitiation, required for nucleotide excisionrepair, similar to 52 kDa subunit of humanTFIIH 0.000210.00314&radic S000004806MRPL24YMR193WverifiedMitochondrial ribosomal protein of the large subunit 0.000210.00314 S000004186PWP1YLR196WverifiedProtein with WD-40 repeats involved in rRNA processing;associates with trans-acting ribosomebiogenesis factors; similar to beta-transducinsuperfamily 0.000210.00314&radic S000004574URA5YML106WverifiedOne of two orotate phosphoribosyltransferase isozymes (see alsoURA10) that catalyze the fifth enzymatic stepin de novo biosynthesis of pyrimidines,converting orotate into orotidine-5'-phosphate 0.000210.00314&radic S000006307PRE2YPR103Wverified20S proteasome beta-type subunit, responsible for thechymotryptic activity of the proteasome 0.000210.00314&radic S000001243RPN10YHR200WverifiedNon-ATPase base subunit of the 19S regulatory particle (RP) ofthe 26S proteasome; N-terminus plays a role inmaintaining the structural integrity of the RP;binds selectively to polyubiquitin chains;homolog of the mammalian S5a protein 0.000210.00314&radic S000005775APC5YOR249CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition 0.000210.00314&radic S000005085AAH1YNL141WverifiedAdenine deaminase (adenine aminohydrolase), involved in purinesalvage and nitrogen catabolism 0.000210.00314&radic S000005424CRT10YOL063CverifiedProtein involved in transcriptional regulation of RNR2 andRNR3; expression of the gene is induced by DNAdamage and null mutations confer increasedresistance to hydroxyurea; N-terminal regionhas a leucine repeat and a WD40 repeat 0.000210.00314 S000002787RGA2YDR379WverifiedGTPase-activating protein for the polarity-establishmentprotein Cdc42p; implicated in control of septinorganization, pheromone response, and haploidinvasive growth 0.000210.00314 S000003768YJR008WUncharacterizedPutative protein of unknown function; expression repressed by inosine and choline in an Opi1p-dependent manner; expression induced by mild heat-stress on a non-fermentable carbon source. 0.000210.00314&radic S000004813ROT1YMR200WverifiedEssential ER membrane protein; may be involved in proteinfolding; mutation causes defects in cell wallsynthesis and in lysis of autophagic bodies,suppresses tor2 mutations, and is syntheticallylethal with kar2-1 and with rot2 mutations 0.000210.00314 S000002238THI3YDL080CverifiedProbable decarboxylase, required for expression of enzymesinvolved in thiamine biosynthesis; may have arole in catabolism of amino acids to long-chainand complex alcohols 0.000210.00314&radic S000003142BUD13YGL174WverifiedSubunit of the RES complex, which is required for nuclearpre-mRNA retention and splicing; involved inbud-site selection; diploid mutants display aunipolar budding pattern instead of thewild-type bipolar pattern 0.000210.0031&radic S000000307SIF2YBR103WverifiedWD40 repeat-containing subunit of the Set3C histone deacetylasecomplex, which represses early/middlesporulation genes; antagonizes telomericsilencing; binds specifically to the Sir4pN-terminus 0.000210.0031&radic S000000523LEU2YCL018WverifiedBeta-isopropylmalate dehydrogenase, catalyzes the third step inthe leucine biosynthesis pathway 0.000210.0031&radic S000001560YKL077WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 0.000210.0031 S000001295BCY1YIL033CverifiedRegulatory subunit of the cyclic AMP-dependent protein kinase(PKA), a component of a signaling pathway thatcontrols a variety of cellular processes,including metabolism, cell cycle, stressresponse, stationary phase, and sporulation 0.000210.0031&radic S000003679TIM17YJL143WverifiedEssential constituent of the mitochondrial inner membranepresequence translocase; interacts with Pam18pto recruit the presequencetranslocase-associated motor (PAM complex) andalso required for protein sorting during import 0.000210.0031&radic S000005072TEP1YNL128WverifiedHomolog of human tumor suppressor gene PTEN/MMAC1/TEP1 that haslipid phosphatase activity and is linked to thephosphatidylinositol signaling pathway; plays arole in normal sporulation 0.000210.0031 S000005739SAS5YOR213CverifiedSubunit of the SAS complex (Sas2p, Sas4p, Sas5p), whichacetylates free histones and nucleosomes andregulates transcriptional silencing; stimulatesSas2p HAT activity 0.000210.0031&radic S000005795PAC1YOR269WverifiedProtein involved in nuclear migration, part of thedynein/dynactin pathway; targets dynein tomicrotubule tips, which is necessary forsliding of microtubules along bud cortex;synthetic lethal with bni1; homolog of humanLIS1 0.000210.0031&radic S000004581DAT1YML113WverifiedDNA binding protein that recognizes oligo(dA).oligo(dT) tracts;Arg side chain in its N-terminal pentadGly-Arg-Lys-Pro-Gly repeat is required forDNA-binding; not essential for viability 0.000210.0031&radic S000002382HBT1YDL223CverifiedSubstrate of the Hub1p ubiquitin-like protein that localizes tothe shmoo tip (mating projection); mutants aredefective for mating projection formation,thereby implicating Hbt1p in polarized cellmorphogenesis 0.000210.0031 S000003035NPY1YGL067WverifiedNADH diphosphatase (pyrophosphatase), hydrolyzes thepyrophosphate linkage in NADH and relatednucleotides; localizes to peroxisomes 0.000210.0031&radic S000000954YER152CUncharacterizedHypothetical protein 0.000210.0031&radic S000001206SOL3YHR163Wverified6-phosphogluconolactonase, catalyzes the second step of thepentose phosphate pathway; weak multicopysuppressor of los1-1 mutation; homologous toSol2p and Sol1p 0.000210.0031&radic S000003484GCN5YGR252WverifiedHistone acetyltransferase, acetylates N-terminal lysines onhistones H2B and H3; catalytic subunit of theADA and SAGA histone acetyltransferasecomplexes; founding member of the Gcn5p-relatedN-acetyltransferase superfamily 0.000210.0031&radic S000005599SGO1YOR073WverifiedComponent of the spindle checkpoint, involved in sensing lackof tension on mitotic chromosomes; protectscentromeric Rec8p at meiosis I; required foraccurate chromosomal segregation at meiosis IIand for mitotic chromosome stability 0.000210.0031&radic S000003483YGR251WUncharacterizedPutative protein of unknown function; deletion mutant has defects in pre-rRNA processing; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the nucleolus; YGR251W is an essential gene 0.000210.0031&radic S000004368PSY3YLR376CuncharacterizedProtein of unknown function; deletion results in a mutatorphenotype suggesting a role for this protein asa mutational suppressor; deletion increasessensitivity to anticancer drugs oxaliplatin andcisplatin but not mitomycin C 0.000210.0031&radic S000003610SMC3YJL074CverifiedSubunit of the multiprotein cohesin complex required for sisterchromatid cohesion in mitotic cells; alsorequired, with Rec8p, for cohesion andrecombination during meiosis; phylogeneticallyconserved SMC chromosomal ATPase family member 0.000210.0031&radic S000003293ADE6YGR061CverifiedFormylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzesa step in the 'de novo' purine nucleotidebiosynthetic pathway 0.000210.0031 S000004647IOC4YMR044WverifiedMember of a complex (Isw1b) with Isw1p and Ioc2p that exhibitsnucleosome-stimulated ATPase activity and actswithin coding regions to coordinatetranscription elongation with termination andprocessing, contains a PWWP motif 0.000210.0031&radic S000003234SWC4YGR002CverifiedProtein of unknown function, component of the Swr1p complexthat incorporates Htz1p into chromatin;component of the NuA4 histone acetyltransferasecomplex 0.000210.0031&radic S000005786GCD1YOR260WverifiedGamma subunit of the translation initiation factor eIF2B, theguanine-nucleotide exchange factor for eIF2;activity subsequently regulated byphosphorylated eIF2; first identified as anegative regulator of GCN4 expression 0.000210.0031 S000002940YDR532CUncharacterizedProtein of unknown function that localizes to the nuclear side of the spindle pole body and along short spindles; deletion mutants have short telomeres; forms a complex with Spc105p 0.000210.0031 S000002598RVB1YDR190CverifiedEssential protein involved in transcription regulation;component of chromatin remodeling complexes;required for assembly and function of the INO80complex; member of the RUVB-like protein family 0.000210.0031&radic S000000354TBS1YBR150CverifiedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.000210.0031&radic S000004997COX5AYNL052WverifiedSubunit Va of cytochrome c oxidase, which is the terminalmember of the mitochondrial inner membraneelectron transport chain; predominantlyexpressed during aerobic growth while itsisoform Vb (Cox5Bp) is expressed duringanaerobic growth 0.000210.0031 S000004632RSF1YMR030WverifiedProtein required for respiratory growth; localized to both thenucleus and mitochondrion; mutant displaysdecreased transcription of specific nuclear andmitochondrial genes whose products are involvedin respiratory growth 0.000210.0031&radic S000003055MMS2YGL087CverifiedProtein involved in error-free postreplication DNA repair;forms a heteromeric complex with Ubc13p thathas a ubiquitin-conjugating activity;cooperates with chromatin-associated RINGfinger proteins, Rad18p and Rad5p 0.000210.0031&radic S000004260DCS1YLR270WverifiedNon-essential hydrolase involved in mRNA decapping, mayfunction in a feedback mechanism to regulatedeadenylation, contains pyrophosphataseactivity and a HIT (histidine triad) motif;interacts with neutral trehalase Nth1p 0.000210.0031&radic S000005633RGS2YOR107WverifiedNegative regulator of glucose-induced cAMP signaling; directlyactivates the GTPase activity of theheterotrimeric G protein alpha subunit Gpa2p 0.000210.0031&radic&radic S000002178RPN4YDL020CverifiedTranscription factor that stimulates expression of proteasomegenes; Rpn4p levels are in turn regulated bythe 26S proteasome in a negative feedbackcontrol mechanism; RPN4 is transcriptionallyregulated by various stress responses 0.000210.0031&radic S000000950SPT15YER148WverifiedTATA-binding protein, general transcription factor thatinteracts with other factors to form thepreinitiation complex at promoters, essentialfor viability 0.000210.0031&radic S000002539YDR132CUncharacterizedHypothetical protein 0.000210.0031&radic S000000961BUR6YER159CverifiedProtein that forms a heterodimeric histone-fold NC2 generaltranscription regulator complex with Ydr1p thatbinds to TBP and represses RNA pol IItranscription during assembly of thepreinitiation complex, homologous to humanNC2alpha 0.000210.0031&radic S000003256THG1YGR024CverifiedtRNAHis guanylyltransferase, adds a guanosine residue to the 5'end of tRNAHis after transcription and RNase Pcleavage; couples nuclear division andmigration to cell budding and cytokinesis;essential enzyme conserved among eukaryotes 0.000210.0031&radic S000005505CTR9YOL145CverifiedComponent of the Paf1p complex, which is a large complex thatbinds to and modulates the activity of RNApolymerase II and is required for expression ofa subset of genes, including cyclin genes;contains TPR repeats 0.000210.0031&radic S000006133PUS1YPL212CverifiedtRNA:pseudouridine synthase, introduces pseudouridines atpositions 26-28, 34-36, 65, and 67 of tRNA;nuclear protein that appears to be involved intRNA export; also acts on U2 snRNA 0.000210.0031&radic S000000823RPN3YER021WverifiedEssential, non-ATPase regulatory subunit of the 26S proteasomelid, similar to the p58 subunit of the human26S proteasome; temperature-sensitive allelescause metaphase arrest, suggesting a role forthe proteasome in cell cycle control 0.000210.0031&radic&radic S000001317YIL055CUncharacterizedHypothetical protein 0.000210.0031 S000000661HCM1YCR065WverifiedForkhead transcription factor that drives S-phase specificexpression of genes involved in chromosomesegregation, spindle dynamics, and budding;suppressor of calmodulin mutants with specificSPB assembly defects; telomere maintenance role 0.000210.0031&radic S000002457TPI1YDR050CverifiedTriose phosphate isomerase, abundant glycolytic enzyme; mRNAhalf-life is regulated by iron availability;transcription is controlled by activatorsReb1p, Gcr1p, and Rap1p through binding sitesin the 5' non-coding region 0.000210.0031&radic S000000589FRM2YCL026C-AverifiedProtein of unknown function, involved in the integration oflipid signaling pathways with cellularhomeostasis 0.000210.0031&radic S000004797YMR185WUncharacterizedProtein required for cell viability 0.000210.00307 S000004413RPN13YLR421CverifiedSubunit of the 19S regulatory particle of the 26S proteasomelid 0.000210.00307&radic S000002518ALT2YDR111CuncharacterizedPutative alanine transaminase (glutamic pyruvic transaminase) 0.000210.00307&radic S000001498PUT3YKL015WverifiedTranscriptional activator of proline utilization genes,constitutively binds PUT1 and PUT2 promotersequences and undergoes a conformational changeto form the active state; has a Zn(2)-Cys(6)binuclear cluster domain 0.000210.00307&radic S000004839TMA29YMR226CverifiedNADP(+)-dependent dehydrogenase; acts on serine,L-allo-threonine, and other 3-hydroxy acids;green fluorescent protein fusion proteinlocalizes to the cytoplasm and nucleus; mayinteract with ribosomes, based onco-purification experiments 0.000210.00307&radic S000004560RAD10YML095CverifiedSingle-stranded DNA endonuclease (with Rad1p), cleavessingle-stranded DNA during nucleotide excisionrepair and double-strand break repair; subunitof Nucleotide Excision Repair Factor 1 (NEF1);homolog of human ERCC1 protein 0.000210.00307&radic&radic S000003187MDM34YGL219CverifiedMitochondrial outer membrane protein, required for normalmitochondrial morphology and inheritance;localizes to dots on the mitochondrial surfacenear mtDNA nucleoids; interacts geneticallywith MDM31 and MDM32 0.000210.00307 S000003715PFD1YJL179WverifiedSubunit of heterohexameric prefoldin, which binds cytosolicchaperonin and transfers target proteins to it;involved in the biogenesis of actin and ofalpha- and gamma-tubulin 0.000210.00307 S000002529KIN1YDR122WverifiedSerine/threonine protein kinase involved in regulation ofexocytosis; localizes to the cytoplasmic faceof the plasma membrane; closely related toKin2p 0.000210.00307 S000006014NOG1YPL093WverifiedPutative GTPase that associates with free 60S ribosomalsubunits in the nucleolus and is required for60S ribosomal subunit biogenesis; constituentof 66S pre-ribosomal particles; member of theODN family of nucleolar G-proteins 0.000210.00307&radic S000003772YJR011CUncharacterizedHypothetical protein 0.000210.00307 S000004671UBX4YMR067CuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p 0.000210.00307&radic S000001358YIL096CUncharacterizedPutative protein of unknown function; associates with precursors of the 60S ribosomal subunit 0.000210.00307&radic S000003891STR2YJR130CverifiedCystathionine gamma-synthase, converts cysteine intocystathionine 0.000210.00307&radic S000003485PUP2YGR253CverifiedAlpha subunit of the 20S proteasome involved inubiquitin-dependent catabolism; human homologis subunit zeta 0.000210.00307&radic S000001782YKR074WUncharacterizedPutative protein of unknown function; epitope-tagged protein localizes to the cytoplasm 0.000210.00307&radic S000004720YMR114CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene 0.000210.00307&radic S000004673NAT4YMR069WverifiedN alpha-acetyl-transferase, involved in acetylation of theN-terminal residues of histones H4 and H2A 0.000210.00307&radic S000001769KTR2YKR061WverifiedMannosyltransferase involved in N-linked protein glycosylation;member of the KRE2/MNT1 mannosyltransferasefamily 0.000210.00307 S000003652NCA3YJL116CverifiedProtein that functions with Nca2p to regulate mitochondrialexpression of subunits 6 (Atp6p) and 8 (Atp8p )of the Fo-F1 ATP synthase; member of the SUNfamily 0.000210.00307 S000004235CDD1YLR245CverifiedCytidine deaminase; catalyzes the modification of cytidine touridine in vitro but native RNA substrates havenot been identified, localizes to both thenucleus and cytoplasm 0.000210.00307&radic S000001412MCM10YIL150CverifiedEssential, chromatin-associated protein involved in theinitiation of DNA replication; required for theassociation of the MCM2-7 complex withreplication origins 0.000210.00307&radic S000003016RPT6YGL048CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; bound byubiquitin-protein ligases Ubr1p and Ufd4p;localized mainly to the nucleus throughout thecell cycle 0.000210.00307&radic S000004172SWI6YLR182WverifiedTranscription cofactor, forms complexes with DNA-bindingproteins Swi4p and Mbp1p to regulatetranscription at the G1/S transition; involvedin meiotic gene expression; localizationregulated by phosphorylation; potential Cdc28psubstrate 0.000210.00307&radic S000006036BEM3YPL115CverifiedRho GTPase activating protein (RhoGAP) involved in control ofthe cytoskeleton organization; targets theessential Rho-GTPase Cdc42p, which controlsestablishment and maintenance of cell polarity,including bud-site assembly 0.000210.00307 S000002771ESC2YDR363WverifiedProtein involved in mating-type locus silencing, interacts withSir2p; probably functions to recruit orstabilize Sir proteins 0.000210.00307&radic S000003358YGR126WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 0.000210.00307&radic&radic S000001716RSC4YKR008WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; found in close proximity tonucleosomal DNA; displaced from the surface ofnucleosomal DNA after chromatin remodeling 0.000210.00307&radic S000000264ORC2YBR060CverifiedSubunit of the origin recognition complex, which directs DNAreplication by binding to replication originsand is also involved in transcriptionalsilencing; may be phosphorylated by Cdc28p 0.000210.00307&radic S000004122YLR132CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to mitochondria and nucleus; YLR132C is an essential gene 0.000210.00307&radic S000003058LIF1YGL090WverifiedProtein involved in DNA double-strand break repair; physicallyinteracts with DNA ligase 4 (Lig4p); homologousto mammalian XRCC4 protein 0.000210.00307&radic S000001788MTD1YKR080WverifiedNAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase,plays a catalytic role in oxidation ofcytoplasmic one-carbon units; expression isregulated by Bas1p and Bas2p, repressed byadenine, and may be induced by inositol andcholine 0.000210.00307&radic S000002761TRR1YDR353WverifiedCytoplasmic thioredoxin reductase, key regulatory enzyme thatdetermines the redox state of the thioredoxinsystem, which acts as a disulfide reductasesystem and protects cells against bothoxidative and reductive stress 0.000210.00307&radic S000004866YMR253CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene 0.000210.00305 S000000904RPS8BYER102WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps8Ap and has similarity to ratS8 ribosomal protein 0.000210.00305 S000002187ARP2YDL029WverifiedEssential component of the Arp2/3 complex, which is a highlyconserved actin nucleation center required forthe motility and integrity of actin patches;involved in endocytosis and membrane growth andpolarity 0.000210.00305 S000002389PTP1YDL230WverifiedPhosphotyrosine-specific protein phosphatase thatdephosphorylates a broad range of substrates invivo, including Fpr3p; localized to thecytoplasm and the mitochondria 0.000210.00305 S000005168SQS1YNL224CverifiedProtein of unknown function; overexpression antagonizes thesuppression of splicing defects by spp382mutants; green fluorescent protein (GFP)-fusionprotein localizes to both the cytoplasm and thenucleus 0.000210.00305&radic S000005861MRS2YOR334WverifiedMitochondrial inner membrane Mg(2+) channel, required formaintenance of intramitochondrial Mg(2+)concentrations at the correct level to supportsplicing of group II introns 0.000210.00305 S000004558UTP14YML093WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000210.00305&radic S000002728SWA2YDR320CverifiedAuxilin-like protein involved in vesicular transport;clathrin-binding protein required for uncoatingof clathrin-coated vesicles 0.000210.00305 S000004927YMR310CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene 0.000210.00305&radic S000006279OPY2YPR075CverifiedIntegral membrane protein that functions in the signalingbranch of the high-osmolarity glycerol (HOG)pathway; interacts with Ste50p; overproductionblocks cell cycle arrest in the presence ofmating pheromone 0.000210.00305 S000002448RSM10YDR041WverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S10 ribosomal protein;essential for viability, unlike most othermitoribosomal proteins 0.000210.00305 S000003601DLS1YJL065CverifiedSubunit of ISW2/yCHRAC chromatin accessibility complex alongwith Itc1p, Isw2p, and Dpb4p; involved ininheritance of telomeric silencing 0.000210.00305&radic S000000360SLI15YBR156CverifiedSubunit of the Ipl1p-Sli15p-Bir1p complex that regulateskinetochore-microtubule attachments, activationof the spindle tension checkpoint, and mitoticspindle disassembly; regulates the activity andlocalization of the Ipl1p aurora kinase 0.000210.00305&radic S000001161SET1YHR119WverifiedHistone methyltransferase, subunit of the COMPASS (Set1C)complex which methylates histone H3 on lysine4; required in transcriptional silencing neartelomeres and at the silent mating type loci;contains a SET domain 0.000210.00305&radic S000000457SRB6YBR253WverifiedEssentital subunit of the RNA polymerase II mediator complex;associates with core polymerase subunits toform the RNA polymerase II holoenzyme;essential for transcriptional regulation 0.000210.00305&radic S000028640YGR204C-AUncharacterizedPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 0.000210.00305 S000001069RPN1YHR027CverifiedNon-ATPase base subunit of the 19S regulatory particle of the26S proteasome; may participate in therecognition of several ligands of theproteasome; contains a leucine-rich repeat(LRR) domain, a site for protein?proteininteractions 0.000210.00305&radic&radic S000005889PRE10YOR362Cverified20S proteasome alpha-type subunit 0.000210.00305&radic S000004680RCO1YMR075WverifiedEssential subunit of the histone deacetylase Rpd3S complex;interacts with Eaf3p 0.000210.00305&radic S000004711PGM2YMR105CverifiedPhosphoglucomutase, catalyzes the conversion fromglucose-1-phosphate to glucose-6-phosphate,which is a key step in hexose metabolism;functions as the acceptor for aGlc-phosphotransferase 0.000210.00305&radic S000000355APD1YBR151WverifiedProtein of unknown function, required for normal localizationof actin patches and for normal tolerance ofsodium ions and hydrogen peroxide; localizes toboth cytoplasm and nucleus 0.000210.00305&radic S000004427TSR2YLR435WverifiedProtein with a potential role in pre-rRNA processing 0.000210.00305&radic S000004319TMA10YLR327CverifiedProtein of unknown function that associates with ribosomes 0.000210.00305&radic S000005591CYT1YOR065WverifiedCytochrome c1, component of the mitochondrial respiratorychain; expression is regulated by theheme-activated, glucose-repressedHap2p/3p/4p/5p CCAAT-binding complex 0.000210.00305 S000002358YDL199CUncharacterizedPutative protein of unknown function with sequence similarity to members of the sugar permease family 0.000210.00305 S000000498SUL1YBR294WverifiedHigh affinity sulfate permease; sulfate uptake is mediated byspecific sulfate transporters Sul1p and Sul2p,which control the concentration of endogenousactivated sulfate intermediates 0.000210.00305 S000000908MAM1YER106WverifiedMonopolin, kinetochore associated protein involved inchromosome attachment to meiotic spindle 0.000210.00305 S000000188RPL32YBL092WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to rat L32 ribosomal protein;overexpression disrupts telomeric silencing 0.000210.00305 S000004096MDN1YLR106CverifiedHuge dynein-related AAA-type ATPase (midasin), forms extendedpre-60S particle with the Rix1 complex(Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependentremodeling of 60S subunits and subsequentexport from nucleoplasm to cytoplasm 0.000210.00305&radic S000000644ARE1YCR048WverifiedAcyl-CoA:sterol acyltransferase, isozyme of Are2p; endoplasmicreticulum enzyme that contributes the majorsterol esterification activity in the absenceof oxygen 0.000210.00305 S000001693UBA1YKL210WverifiedUbiquitin activating enzyme, involved in ubiquitin-mediatedprotein degradation and essential for viability 0.000210.00305&radic S000004143ACS2YLR153CverifiedAcetyl-coA synthetase isoform which, along with Acs1p, is thenuclear source of acetyl-coA for histoneacetlyation; mutants affect globaltranscription; required for growth on glucose;expressed under anaerobic conditions 0.000210.00305&radic S000000399MSI1YBR195CverifiedSubunit of chromatin assembly factor I (CAF-I), regulates theRAS/cAMP pathway via sequestration of Npr1pkinase; localizes to the nucleus and cytoplasm;homologous to human retinoblastoma bindingproteins RbAp48 and RbAp46 0.000210.00305&radic S000007245COX19YLL018C-AverifiedProtein required for cytochrome c oxidase assembly, located inthe cytosol and mitochondrial intermembranespace; putative copper metallochaperone thatdelivers copper to cytochrome c oxidase 0.000210.00305&radic S000001800SRP40YKR092CverifiedNucleolar, serine-rich protein with a role in preribosomeassembly or transport; may function as achaperone of small nucleolar ribonucleoproteinparticles (snoRNPs); immunologically andstructurally to rat Nopp140 0.000210.00305&radic S000005717RIS1YOR191WverifiedMember of the SWI/SNF family of DNA-dependent ATPases, plays arole in antagonizing silencing duringmating-type switching, contains an N-terminaldomain that interacts with Sir4p and aC-terminal SNF2 domain 0.000210.00305&radic S000005967ELC1YPL046CverifiedElongin C, forms heterodimer with Ela1p that participates intranscription elongation; expressiondramatically upregulated during sporulation;widely conserved among eukaryotes 0.000210.00305&radic S000001076PIH1YHR034CverifiedProtein of unresolved function; may function in protein foldingand/or rRNA processing, interacts with achaperone (Hsp82p), two chromatin remodelingfactors (Rvb1p, Rvb2p) and two rRNA processingfactors (Rrp43p, Nop58p) 0.000210.00305&radic S000007472HUG1YML058W-AverifiedProtein involved in the Mec1p-mediated checkpoint pathway thatresponds to DNA damage or replication arrest,transcription is induced by DNA damage 0.000210.00305 S000002333DLD1YDL174CverifiedD-lactate dehydrogenase, oxidizes D-lactate to pyruvate,transcription is heme-dependent, repressed byglucose, and derepressed in ethanol or lactate;located in the mitochondrial inner membrane 0.000210.00305 S000005051YAF9YNL107WverifiedSubunit of both the NuA4 histone H4 acetyltransferase complexand the SWR1 complex, may function toantagonize silencing near telomeres; interactsdirectly with Swc4p, has homology to humanleukemogenic protein AF9, contains a YEATSdomain 0.000210.00305&radic S000001118PTC7YHR076WverifiedMitochondrially localized type 2C protein phosphatase;expression induced by growth on ethanol and bysustained osmotic stress; possible role incarbon source utilization in low oxygenenvironments 0.000210.00305 S000005734PTP2YOR208WverifiedPhosphotyrosine-specific protein phosphatase involved in theinactivation of mitogen-activated proteinkinase (MAPK) during osmolarity sensing;dephosporylates Hog1p MAPK and regulates itslocalization; localized to the nucleus 0.000210.00305&radic S000001695SAC1YKL212WverifiedLipid phosphoinositide phosphatase of the ER and Golgi,involved in protein trafficking and secretion 0.000210.00305 S000003514BGL2YGR282CverifiedEndo-beta-1,3-glucanase, major protein of the cell wall,involved in cell wall maintenance 0.000210.00305 S000005650UBP2YOR124CverifiedUbiquitin-specific protease that removes ubiquitin fromubiquitinated proteins, cleaves at the Cterminus of ubiquitin fusions; capable ofcleaving polyubiquitin and possessesisopeptidase activity 0.000210.00305 S000000117HAP3YBL021CverifiedSubunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5pCCAAT-binding complex, a transcriptionalactivator and global regulator of respiratorygene expression; contains sequencescontributing to both complex assembly and DNAbinding 0.000210.00305&radic S000005483HRP1YOL123WverifiedSubunit of cleavage factor I, a five-subunit complex requiredfor the cleavage and polyadenylation ofpre-mRNA 3' ends; RRM-containing heteronuclearRNA binding protein and hnRNPA/B family memberthat binds to poly (A) signal sequences 0.000210.00305&radic S000002987CKB1YGL019WverifiedBeta regulatory subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA1, CKA2 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 0.000210.00302&radic S000003657RPE1YJL121CverifiedD-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in thenon-oxidative part of the pentose-phosphatepathway; mutants are sensitive to oxidativestress 0.000210.00302&radic S000005913PHR1YOR386WverifiedDNA photolyase involved in photoreactivation, repairspyrimidine dimers in the presence of visiblelight; induced by DNA damage; regulated bytranscriptional repressor Rph1p 0.000210.00302&radic<