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Prediction of GO:0005935 "bud neck"

GO branch: CC
Number of currently annotated genes: 109

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest Huh:bud neckLum:DoxycyclineLum:CymoxanilHuh:ambiguousLum:PromethazineHuh:cell peripheryTAP:107Huh:cytoplasmLum:WarfarinHuh:nucleus S000000595CDC10YCR002CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM &radic0.824250.95238&radic&radic&radic&radic&radic&radic S000001149CDC12YHR107CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM &radic0.758570.93227&radic&radic&radic&radic&radic S000002384SHS1YDL225WverifiedOne of five related septins (Cdc3p, Cdc10p, Cdc11p, Cdc12p,Shs1p) that form a cortical filamentous collarat the mother-bud neck which is necessary fornormal morphogenesis and cytokinesis 0.756870.93227&radic&radic S000004306CDC3YLR314CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM &radic0.731260.93061&radic&radic&radic S000003837CDC11YJR076CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM &radic0.665970.92919&radic&radic S000001065MYO1YHR023WverifiedType II myosin heavy chain, required for wild-type cytokinesisand cell separation; localizes to theactomyosin ring; binds to myosin light chainsMlc1p and Mlc2p through its IQ1 and IQ2 motifsrespectively &radic0.651430.92919&radic&radic S000006259SEC8YPR055WverifiedEssential 121kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 0.612810.92874&radic&radic&radic&radic S000006163IQG1YPL242CverifiedEssential protein required for determination of buddingpattern, promotes localization of axial markersBud4p and Cdc12p and functionally interactswith Sec3p, localizes to the contractile ringduring anaphase, member of the IQGAP family &radic0.60710.92874&radic&radic&radic&radic&radic S000000520BUD3YCL014WverifiedProtein involved in bud-site selection and required for axialbudding pattern; localizes with septins to budneck in mitosis and may constitute an axiallandmark for next round of budding &radic0.585810.92803&radic&radic&radic&radic S000005853MYO2YOR326WverifiedOne of two type V myosin motors (along with MYO4) involved inactin-based transport of cargos; required forthe polarized delivery of secretory vesicles,the vacuole, late Golgi elements, peroxisomes,and the mitotic spindle &radic0.566520.92623&radic&radic&radic&radic&radic&radic&radic&radic S000002915GIN4YDR507CverifiedProtein kinase involved in bud growth and assembly of theseptin ring, proposed to have kinase-dependentand kinase-independent activities; undergoesautophosphorylation; similar to Kcc4p and Hsl1p &radic0.567850.92623&radic&radic&radic S000003202SEC15YGL233WverifiedEssential 113kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which mediates polarized targeting ofvesicles to active sites of exocytosis; Sec15passociates with Sec4p and vesicles 0.566090.92479&radic&radic&radic&radic&radic&radic S000005215BNI1YNL271CverifiedFormin, nucleates the formation of linear actin filaments,involved in cell processes such as budding andmitotic spindle orientation which require theformation of polarized actin cables,functionally redundant with BNR1 &radic0.554250.9168&radic&radic&radic&radic S000004156SEC10YLR166CverifiedEssential 100kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 0.54930.91357&radic&radic&radic&radic&radic S000000404BEM1YBR200WverifiedProtein containing SH3-domains, involved in establishing cellpolarity and morphogenesis; functions as ascaffold protein for complexes that includeCdc24p, Ste5p, Ste20p, and Rsr1p &radic0.541040.91061&radic&radic&radic&radic&radic&radic&radic S000006392MLC2YPR188CuncharacterizedRegulatory light chain for the type II myosin, Myo1p; binds toan IQ motif of Myo1p, localization to the budneck depends on Myo1p; involved in thedisassembly of the Myo1p ring &radic0.522540.90896&radic&radic&radic S000001584HSL1YKL101WverifiedNim1p-related protein kinase that regulates the morphogenesisand septin checkpoints; associates with theassembled septin filament; required along withHsl7p for bud neck recruitment,phosphorylation, and degradation of Swe1p &radic0.513090.90651&radic&radic&radic&radic&radic S000004603CDC5YMR001CverifiedPolo-like kinase with similarity to Xenopus Plx1 and S. pombePlo1p; found at bud neck, nucleus and SPBs; hasmultiple functions in mitosis and cytokinesisthrough phosphorylation of substrates; may be aCdc28p substrate &radic0.514040.90651&radic&radic&radic&radic&radic S000000337HSL7YBR133CverifiedProtein arginine N-methyltransferase that exhibits septin andHsl1p-dependent bud neck localization andperiodic Hsl1p-dependent phosphorylation;required along with Hsl1p for bud neckrecruitment, phosphorylation, and degradationof Swe1p &radic0.509270.90651&radic&radic S000003621EXO70YJL085WverifiedEssential 70kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 0.50640.90604&radic&radic S000000529KCC4YCL024WverifiedProtein kinase of the bud neck involved in the septincheckpoint, associates with septin proteins,negatively regulates Swe1p by phosphorylation,shows structural homology to bud neck kinasesGin4p and Hsl1p &radic0.500260.90588&radic&radic&radic&radic&radic&radic S000001421BNR1YIL159WverifiedFormin, nucleates the formation of linear actin filaments,involved in cell processes such as budding andmitotic spindle orientation which require theformation of polarized actin cables,functionally redundant with BNI1 &radic0.498380.90588&radic S000001330SEC6YIL068CverifiedEssential 88kDa subunit of the exocyst complex, which mediatespolarized targeting of secretory vesicles toactive sites of exocytosis; dimeric form ofSec6p interacts with Sec9p in vitro andinhibits t-SNARE assembly 0.493130.90436&radic&radic&radic&radic&radic S000005714MSB1YOR188WverifiedProtein involved in positive regulation of both 1,3-beta-glucansynthesis and the Pkc1p-MAPK pathway, potentialCdc28p substrate; multicopy suppressor oftemperature-sensitive mutations in CDC24 andCDC42, and of mutations in BEM4 &radic0.488240.90029&radic&radic&radic&radic S000004635HOF1YMR032WverifiedBud neck-localized, SH3 domain-containing protein required forcytokinesis; regulates actomyosin ring dynamicsand septin localization; interacts with theformins, Bni1p and Bnr1p, and with Cyk3p,Vrp1p, and Bni5p &radic0.487450.89976&radic&radic&radic&radic S000005177BNI4YNL233WverifiedTargeting subunit for Glc7p protein phosphatase, localized tothe bud neck, required for localization ofchitin synthase III to the bud neck viainteraction with the chitin synthase IIIregulatory subunit Skt5p &radic0.480680.89705&radic&radic&radic&radic&radic&radic S000003324DBF2YGR092WverifiedSer/Thr kinase involved in transcription and stress response;functions as part of a network of genes in exitfrom mitosis; localization is cell cycleregulated; activated by Cdc15p during the exitfrom mitosis &radic0.47960.89403&radic&radic S000002573SEC5YDR166CverifiedEssential 107kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis &radic0.477260.89269&radic&radic&radic&radic&radic&radic&radic S000000810SEC3YER008CverifiedNon-essential subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p)which mediates targeting of post-Golgi vesiclesto sites of active exocytosis; Sec3pspecifically is a spatial landmark forsecretion &radic0.452950.8843&radic&radic&radic&radic&radic&radic S000001531ELM1YKL048CverifiedSerine/threonine protein kinase that regulates cellularmorphogenesis, septin behavior, andcytokinesis; required for the regulation ofother kinases; forms part of the bud neck ring &radic0.452220.88381&radic&radic&radic&radic S000004345BUD8YLR353WverifiedProtein involved in bud-site selection; diploid mutants displaya unipolar budding pattern instead of thewild-type bipolar pattern, and bud at theproximal pole 0.450.88362&radic&radic&radic&radic&radic&radic S000000957BEM2YER155CverifiedRho GTPase activating protein (RhoGAP) involved in the controlof cytoskeleton organization and cellularmorphogenesis; required for bud emergence 0.445250.8801&radic&radic S000004311BUD6YLR319CverifiedActin- and formin-interacting protein, involved in actin cablenucleation and polarized cell growth; isolatedas bipolar budding mutant; potential Cdc28psubstrate 0.444360.8789&radic&radic&radic&radic S000000916BOI2YER114CverifiedProtein implicated in polar growth, functionally redundant withBoi1p; interacts with bud-emergence proteinBem1p; contains an SH3 (src homology 3) domainand a PH (pleckstrin homology) domain &radic0.440560.87506&radic&radic&radic S000005653RGA1YOR127WverifiedGTPase-activating protein for the polarity-establishmentprotein Cdc42p; implicated in control of septinorganization, pheromone response, and haploidinvasive growth 0.440540.87506&radic&radic&radic S000005472MSB4YOL112WverifiedGTPase-activating protein of the Ras superfamily that actsprimarily on Sec4p, localizes to the bud siteand bud tip, has similarity to Msb3p; msb3 msb4double mutation causes defects in secretion andactin organization 0.437670.87373&radic&radic&radic S000000027MYO4YAL029CverifiedOne of two type V myosin motors (along with MYO2) involved inactin-based transport of cargos; required formRNA transport, including ASH1 mRNA, andfacilitating the growth and movement of ERtubules into the growing bud along with She3p 0.437320.87336&radic&radic&radic&radic&radic&radic&radic&radic S000003852BUD4YJR092WverifiedProtein involved in bud-site selection and required for axialbudding pattern; localizes with septins to budneck in mitosis and may constitute an axiallandmark for next round of budding; potentialCdc28p substrate &radic0.430540.86775&radic S000005242CLA4YNL298WverifiedCdc42p activated signal transducing kinase of the PAK(p21-activated kinase) family, involved inseptin ring assembly and cytokinesis; directlyphosphorylates septins Cdc3p and Cdc10p; otheryeast PAK family members are Ste20p and Skm1p 0.426770.86426&radic&radic&radic&radic&radic S000000313CMD1YBR109CverifiedCalmodulin; Ca++ binding protein that regulates Ca++independent processes (mitosis, bud growth,actin organization, endocytosis, etc.) and Ca++dependent processes (stress-activatedpathways), targets include Nuf1p, Myo2p andcalcineurin &radic0.422690.86089&radic&radic S000005332MSO1YNR049CverifiedProbable component of the secretory vesicle docking complex,acts at a late step in secretion; shows geneticand physical interactions with Sec1p and isenriched in microsomal membrane fractions;required for sporulation 0.418540.85835&radic&radic&radic S000000157SKT5YBL061CverifiedActivator of Chs3p (chitin synthase III), recruits Chs3p to thebud neck via interaction with Bni4p; hassimilarity to Shc1p, which activates Chs3pduring sporulation &radic0.418450.85835&radic&radic&radic&radic&radic&radic S000006125HRR25YPL204WverifiedProtein kinase involved in regulating diverse events includingvesicular trafficking, gene expression, DNArepair, and chromosome segregation; binds theCTD of RNA pol II; homolog of mammalian caseinkinase 1delta (CK1delta) 0.41860.85835&radic&radic&radic&radic&radic&radic&radic S000004804GYL1YMR192WverifiedPutative GTPase activating protein (GAP) that may have a rolein polarized exocytosis; stimulates Gyp5p GAPactivity on Ypt1p, colocalizes with Gyp5p atsites of polarized growth; interacts withGyp5p, Rvs161p, and Rvs167p &radic0.415830.85758&radic&radic&radic&radic&radic S000005216SEC2YNL272CverifiedGuanyl-nucleotide exchange factor for the small G-proteinSec4p, located on cytoplasmic vesicles;essential for post-Golgi vesicle transport 0.411870.85478&radic&radic&radic&radic&radic S000004363ROM2YLR371WverifiedGDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutationsare synthetically lethal with mutations inrom1, which also encodes a GEP 0.411460.85305&radic&radic S000002186MPS1YDL028CverifiedDual-specificity kinase required for spindle pole body (SPB)duplication and spindle checkpoint function;substrates include SPB proteins Spc42p,Spc110p, and Spc98p, mitotic exit networkprotein Mob1p, and checkpoint protein Mad1p 0.408730.8504&radic S000000181BOI1YBL085WverifiedProtein implicated in polar growth, functionally redundant withBoi2p; interacts with bud-emergence proteinBem1p; contains an SH3 (src homology 3) domainand a PH (pleckstrin homology) domain &radic0.406820.84815&radic&radic&radic&radic&radic&radic S000000306EXO84YBR102CverifiedEssential protein with dual roles in spliceosome assembly andexocytosis; the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p) mediates polarized targeting ofsecretory vesicles to active sites ofexocytosis &radic0.402280.84515&radic&radic&radic S000005110BNI5YNL166CverifiedProtein involved in organization of septins at the mother-budneck, may interact directly with the Cdc11pseptin, localizes to bud neck in aseptin-dependent manner &radic0.397880.84031&radic&radic&radic S000001201KEL1YHR158CverifiedProtein required for proper cell fusion and cell morphology;functions in a complex with Kel2p to negativelyregulate mitotic exit, interacts with Tem1p andLte1p; localizes to regions of polarizedgrowth; potential Cdc28p substrate &radic0.397240.83913&radic&radic&radic S000005790DSE3YOR264WverifiedDaughter cell-specific protein, may help establish daughterfate &radic0.396250.83843&radic&radic&radic&radic S000002571SEC1YDR164CverifiedSm-like protein involved in docking and fusion of exocyticvesicles through binding to assembled SNAREcomplexes at the membrane; localization tosites of secretion (bud neck and bud tip) isdependent on SNARE function &radic0.39270.83613&radic&radic&radic&radic&radic&radic S000006036BEM3YPL115CverifiedRho GTPase activating protein (RhoGAP) involved in control ofthe cytoskeleton organization; targets theessential Rho-GTPase Cdc42p, which controlsestablishment and maintenance of cell polarity,including bud-site assembly 0.391880.83419&radic&radic&radic&radic S000000201PKC1YBL105CverifiedProtein serine/threonine kinase essential for cell wallremodeling during growth; localized to sites ofpolarized growth and the mother-daughter budneck; homolog of the alpha, beta, and gammaisoforms of mammalian protein kinase C (PKC) 0.386740.82934&radic&radic&radic S000006170GYP5YPL249CverifiedGTPase-activating protein (GAP) for yeast Rab family members,involved in ER to Golgi trafficking; exhibitsGAP activity toward Ypt1p that is stimulated byGyl1p, also acts on Sec4p; interacts withGyl1p, Rvs161p and Rvs167p &radic0.38560.82916&radic&radic&radic&radic&radic&radic S000006326AXL1YPR122WverifiedHaploid specific endoprotease that performs one of twoN-terminal cleavages during maturation ofa-factor mating pheromone; required for axialbudding pattern of haploid cells &radic0.378650.82274&radic&radic&radic&radic&radic S000003273BUD9YGR041WverifiedProtein involved in bud-site selection; diploid mutants displaya unipolar budding pattern instead of thewild-type bipolar pattern, and bud at thedistal pole &radic0.373440.8206&radic&radic&radic&radic&radic&radic S000002275CYK3YDL117WverifiedSH3-domain protein located in the mother-bud neck and thecytokinetic actin ring; mutant phenotype andgenetic interactions suggest a role incytokinesis &radic0.367470.81499&radic&radic S000000464RGD1YBR260CverifiedGTPase-activating protein (RhoGAP) for Rho3p and Rho4p,possibly involved in control of actincytoskeleton organization 0.36320.81302&radic&radic&radic S000005987YPL066WUncharacterizedHypothetical protein &radic0.358250.80925&radic&radic&radic&radic&radic S000000634BUD5YCR038CverifiedGTP/GDP exchange factor for Rsr1p (Bud1p) required for bothaxial and bipolar budding patterns; mutantsexhibit random budding in all cell types &radic0.355640.80821&radic&radic S000005022NIS1YNL078WverifiedProtein localized in the bud neck at G2/M phase; physicallyinteracts with septins; possibly involved in amitotic signaling network &radic0.354410.8074&radic&radic S000005534SLG1YOR008CverifiedSensor-transducer of the stress-activated PKC1-MPK1 kinasepathway involved in maintenance of cell wallintegrity; involved in organization of theactin cytoskeleton; secretory pathway Wsc1p isrequired for the arrest of secretion response 0.353670.80552&radic&radic&radic S000000626SYP1YCR030CverifiedProtein with a potential role in actin cytoskeletalorganization; overexpression suppresses a pfy1(profilin) null mutation &radic0.351830.80366&radic&radic S000005431NBA1YOL070CverifiedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cellperiphery, cytoplasm, and bud neck; potentialCdc28p substrate &radic0.344340.79827&radic&radic&radic&radic&radic S000001402AXL2YIL140WverifiedIntegral plasma membrane protein required for axial budding inhaploid cells, localizes to the incipient budsite and bud neck; glycosylated by Pmt4p;potential Cdc28p substrate &radic0.34040.79496&radic&radic&radic S000001798PXL1YKR090WverifiedLIM domain-containing protein that localizes to sites ofpolarized growth, required for selection and/ormaintenance of polarized growth sites, maymodulate signaling by the GTPases Cdc42p andRho1p; has similarity to metazoan paxillin &radic0.330710.79099&radic&radic&radic&radic S000002717GIC2YDR309CverifiedProtein of unknown function involved in initiation of buddingand cellular polarization, interacts withCdc42p via the Cdc42/Rac-interactive binding(CRIB) domain 0.328750.7871&radic&radic&radic&radic&radic S000000926DSE1YER124CverifiedDaughter cell-specific protein, may participate in pathwaysregulating cell wall metabolism; deletionaffects cell separation after division andsensitivity to drugs targeted against the cellwall &radic0.324430.78578&radic S000001184CHS7YHR142WverifiedProtein of unknown function, involved in chitin biosynthesis byregulating Chs3p export from the ER 0.324940.78578&radic&radic&radic S000000920SHO1YER118CverifiedTransmembrane osmosensor, participates in activation of boththe Cdc42p- and MAP kinase-dependentfilamentous growth pathway and thehigh-osmolarity glycerol response pathway 0.324370.78555&radic&radic&radic S000004074RAX2YLR084CverifiedN-glycosylated protein involved in the maintenance of bud siteselection during bipolar budding; localizationrequires Rax1p &radic0.309410.77569&radic&radic S000001575BUD2YKL092CverifiedGTPase activating factor for Rsr1p/Bud1p required for bothaxial and bipolar budding patterns; mutantsexhibit random budding in all cell types 0.288020.75727&radic&radic&radic&radic&radic&radic S000003291SPR3YGR059WverifiedSporulation-specific homolog of the yeast CDC3/10/11/12 familyof bud neck microfilament genes; septin proteininvolved in sporulation; regulated by ABFI &radic0.28420.75454&radic&radic&radic&radic S000002399LRG1YDL240WverifiedPutative GTPase-activating protein (GAP) involved in thePkc1p-mediated signaling pathway that controlscell wall integrity; appears to specificallyregulate 1,3-beta-glucan synthesis &radic0.27930.74689&radic&radic&radic S000000834FIR1YER032WverifiedProtein involved in 3' mRNA processing, interacts with Ref2p;potential Cdc28p substrate &radic0.278840.7467&radic&radic&radic&radic&radic S000005105CBK1YNL161WverifiedSerine/threonine protein kinase that regulates cellmorphogenesis pathways; involved in cell wallbiosynthesis, apical growth, proper matingprojection morphology, bipolar bud siteselection in diploid cells, and cell separation &radic0.26870.73492&radic&radic&radic&radic&radic&radic S000004731YMR124WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene 0.268550.73399&radic&radic&radic&radic S000002626SPR28YDR218CverifiedSporulation-specific homolog of the yeast CDC3/10/11/12 familyof bud neck microfilament genes; meiotic septinexpressed at high levels during meioticdivisions and ascospore formation &radic0.26680.7323&radic&radic&radic S000003723SWE1YJL187CverifiedProtein kinase that regulates the G2/M transition by inhibitionof Cdc28p kinase activity; localizes to thenucleus and to the daughter side of themother-bud neck; homolog of S. pombe Wee1p;potential Cdc28p substrate &radic0.260480.72413&radic&radic&radic&radic S000000951PEA2YER149CverifiedCoiled-coil polarisome protein required for polarizedmorphogenesis, cell fusion, and low affinityCa2+ influx; forms polarisome complex withBni1p, Bud6p, and Spa2p; localizes to sites ofpolarized growth 0.258490.7223&radic&radic S000005187SLA2YNL243WverifiedTransmembrane actin-binding protein involved in membranecytoskeleton assembly and cell polarization;adaptor protein that links actin to clathrinand endocytosis; present in the actin corticalpatch of the emerging bud tip; dimer in vivo 0.245830.71068 S000001380RHO3YIL118WverifiedNon-essential small GTPase of the Rho/Rac subfamily of Ras-likeproteins involved in the establishment of cellpolarity; GTPase activity positively regulatedby the GTPase activating protein (GAP) Rgd1p 0.241480.70333 S000006037HOS3YPL116WverifiedTrichostatin A-insensitive homodimeric histone deacetylase(HDAC) with specificity in vitro for histonesH3, H4, H2A, and H2B; similar to Hda1p, Rpd3p,Hos1p, and Hos2p; deletion results in increasedhistone acetylation at rDNA repeats &radic0.23930.70006&radic&radic&radic S000003074MLC1YGL106WverifiedEssential light chain for myosin Myo2p; may stabilize Myo2p bybinding to the neck region; may interact withMyo1p, Iqg1p, and Myo2p to coordinate formationand contraction of the actomyosin ring withtargeted membrane deposition &radic0.233480.69404&radic S000002968LSB3YFR024C-AverifiedProtein containing a C-terminal SH3 domain; binds Las17p, whichis a homolog of human Wiskott-Aldrich Syndromeprotein involved in actin patch assembly andactin polymerization &radic0.228260.68747&radic&radic&radic&radic S000003473YAP1802YGR241CverifiedProtein involved in clathrin cage assembly; binds Pan1p andclathrin; homologous to Yap1801p, member of theAP180 protein family 0.221970.67843&radic&radic&radic&radic&radic S000006315DBF20YPR111WverifiedSer/Thr kinase involved in late nuclear division, one of themitotic exit network (MEN) proteins; necessaryfor the execution of cytokinesis 0.213870.66627&radic&radic&radic&radic&radic S000001847RGD2YFL047WverifiedGTPase-activating protein (RhoGAP) for Cdc42p and Rho5p &radic0.213590.66434&radic&radic&radic&radic&radic S000000103SLA1YBL007CverifiedCytoskeletal protein binding protein required for assembly ofthe cortical actin cytoskeleton; contains 3 SH3domains; interacts with proteins regulatingactin dynamics and with proteins required forendocytosis 0.211750.66206&radic&radic&radic S000000999STE20YHL007CverifiedSignal transducing kinase of the PAK (p21-activated kinase)family, involved in pheromone response andpseudohyphal/invasive growth pathways,activated by Cdc42p; binds Ste4p at a GBB motifpresent in noncatalytic domains of PAK kinases 0.203150.65141&radic&radic&radic&radic&radic S000006079YPL158CUncharacterizedHypothetical protein &radic0.200110.64591&radic S000001763RHO4YKR055WverifiedNon-essential small GTPase of the Rho/Rac subfamily of Ras-likeproteins, likely to be involved in theestablishment of cell polarity 0.196740.64084&radic&radic&radic S000001192SKG6YHR149CuncharacterizedProtein of unknown function; found in the bud tip and bud neck,potential Cdc28p substrate; Skg6p interactswith Zds1p and Zds2p &radic0.196550.64051&radic&radic&radic S000006323CLB2YPR119WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome &radic0.196370.63985&radic&radic&radic&radic S000004715MYO5YMR109WverifiedOne of two type I myosins; contains proline-rich tail homology2 (TH2) and SH3 domains; MYO5 deletion haslittle effect on growth, but myo3 myo5 doubledeletion causes severe defects in growth andactin cytoskeleton organization 0.188490.62739 S000002292PPH21YDL134CverifiedCatalytic subunit of protein phosphatase 2A, functionallyredundant with Pph22p; methylated at Cterminus; forms alternate complexes withseveral regulatory subunits; involved in signaltransduction and regulation of mitosis 0.18820.62703&radic&radic S000000334SHE3YBR130CverifiedProtein that acts as an adaptor between Myo4p and theShe2p-mRNA complex; part of the mRNAlocalization machinery that restrictsaccumulation of certain proteins to the bud;also required for cortical ER inheritance 0.187490.62638&radic&radic&radic&radic S000005423APM4YOL062CverifiedMu2-like subunit of the clathrin associated protein complex(AP-2); involved in vesicle transport 0.186330.62286&radic&radic&radic&radic&radic S000000022LTE1YAL024CverifiedPutative GDP/GTP exchange factor required for mitotic exit atlow temperatures; acts as a guanine nucleotideexchange factor (GEF) for Tem1p, which is a keyregulator of mitotic exit; physicallyassociates with Ras2p-GTP 0.186250.62286&radic S000000156YBL060WUncharacterizedHypothetical protein &radic0.176260.60494&radic&radic&radic&radic S000000039CDC24YAL041WverifiedGuanine nucleotide exchange factor (GEF or GDP-release factor)for Cdc42p; required for polarity establishmentand maintenance, and mutants have morphologicaldefects in bud formation and shmooing &radic0.17540.60297&radic&radic&radic&radic S000005953SVL3YPL032CverifiedProtein of unknown function, mutant phenotype suggests apotential role in vacuolar function; greenfluorescent protein (GFP)-fusion proteinlocalizes to the cell periphery, cytoplasm,bud, and bud neck &radic0.17460.60243&radic&radic&radic&radic&radic&radic&radic&radic S000006236SRO7YPR032WverifiedPutative effector of Rab GTPase Sec4p, forms a complex withSec4p and t-SNARE Sec9p; involved in exocytosisand docking and fusion of post-Golgi vesicleswith plasma membrane; homolog of Sro77p andDrosophila lgl tumor suppressor 0.171840.59806&radic&radic S000004086KIN2YLR096WverifiedSerine/threonine protein kinase involved in regulation ofexocytosis; localizes to the cytoplasmic faceof the plasma membrane; closely related toKin1p 0.171110.59651&radic&radic&radic&radic&radic S000005222CAF120YNL278WverifiedPart of the evolutionarily-conserved CCR4-NOT transcriptionalregulatory complex involved in controlling mRNAinitiation, elongation, and degradation 0.170760.59615&radic&radic&radic&radic&radic&radic S000001889SEC4YFL005WverifiedSecretory vesicle-associated Rab GTPase essential forexocytosis; associates with the exocystcomponent Sec15p and may regulate polarizeddelivery of transport vesicles to the exocystat the plasma membrane 0.170520.59552 S000002536SAC6YDR129CverifiedFimbrin, actin-bundling protein; cooperates with Scp1p(calponin/transgelin) in the organization andmaintenance of the actin cytoskeleton 0.170140.59461&radic&radic&radic S000001562SMY1YKL079WverifiedProtein that interacts with Myo2p, proposed to be involved inexocytosis; N-terminal domain is related to themotor domain of kinesins &radic0.169990.59394&radic&radic&radic&radic&radic S000001612MYO3YKL129CverifiedOne of two type I myosins; localizes to actin cortical patches;deletion of MYO3 has little effect on growth,but myo3 myo5 double deletion causes severedefects in growth and actin cytoskeletonorganization 0.16670.58724&radic&radic&radic&radic S000001673CNB1YKL190WverifiedCalcineurin B; the regulatory subunit of calcineurin, aCa++/calmodulin-regulated protein phosphatasewhich regulates Crz1p (a stress-responsetranscription factor), the other calcineurinsubunit is encoded by CNA1 and/or CMP1 0.166820.58724&radic&radic S000004798HSC82YMR186WverifiedCytoplasmic chaperone of the Hsp90 family, redundant infunction and nearly identical with Hsp82p, andtogether they are essential; expressedconstitutively at 10-fold higher basal levelsthat HSP82 and induced 2-3 fold by heat shock 0.16590.58474&radic&radic&radic&radic S000001204YAP1801YHR161CverifiedProtein involved in clathrin cage assembly; binds Pan1p andclathrin; homologous to Yap1802p, member of theAP180 protein family 0.165420.58467&radic&radic&radic&radic&radic S000000133APL3YBL037WverifiedAlpha-adaptin, large subunit of the clathrin associated proteincomplex (AP-2); involved in vesicle mediatedtransport 0.165140.5839&radic&radic S000003420BUB1YGR188CverifiedProtein kinase that forms a complex with Mad1p and Bub3p thatis crucial in the checkpoint mechanism requiredto prevent cell cycle progression into anaphasein the presence of spindle damage, associateswith centromere DNA via Skp1p 0.164150.58188&radic S000003470KEL2YGR238CverifiedProtein that functions in a complex with Kel1p to negativelyregulate mitotic exit, interacts with Tem1p andLte1p; localizes to regions of polarizedgrowth; potential Cdc28p substrate &radic0.163690.58167&radic&radic S000001156BZZ1YHR114WverifiedSH3 domain protein implicated in the regulation of actinpolymerization, able to recruit actinpolymerization machinery through its SH3domains, colocalizes with cortical actinpatches and Las17p, interacts with type Imyosins 0.162860.58067&radic&radic S000003765APL1YJR005WverifiedBeta-adaptin, large subunit of the clathrin associated proteincomplex (AP-2); involved in vesicle mediatedtransport; similar to mammalian beta-chain ofthe clathrin associated protein complex 0.16190.5796&radic&radic S000002756YDR348CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud neck; potential Cdc28p substrate &radic0.15990.5764&radic&radic&radic S000000263AKL1YBR059CverifiedSer-Thr protein kinase, member (with Ark1p and Prk1p) of theArk kinase family; involved in endocytosis andactin cytoskeleton organization &radic0.157510.57267&radic&radic&radic S000006142FLC1YPL221WverifiedPutative FAD transporter; required for uptake of FAD intoendoplasmic reticulum; involved in cell wallmaintenance &radic0.157470.57249&radic&radic&radic&radic&radic&radic S000004177SKG3YLR187WuncharacterizedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cellperiphery, cytoplasm, bud, and bud neck;potential Cdc28p substrate; similar to Caf120pand Skg4p &radic0.156960.57092&radic&radic&radic&radic&radic&radic&radic&radic S000005830YOR304C-AUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck &radic0.156150.56989&radic&radic&radic&radic&radic&radic S000001518UGP1YKL035WverifiedUDP-glucose pyrophosphorylase (UGPase), catalyses thereversible formation of UDP-Glc from glucose1-phosphate and UTP, involved in a wide varietyof metabolic pathways, expression modulated byPho85p through Pho4p 0.155140.5687&radic&radic&radic&radic S000002347PPH22YDL188CverifiedCatalytic subunit of protein phosphatase 2A, functionallyredundant with Pph21p; methylated at Cterminus; forms alternate complexes withseveral regulatory subunits; involved in signaltransduction and regulation of mitosis 0.154650.56788&radic&radic S000003819APS2YJR058CverifiedSmall subunit of the clathrin-associated adaptor complex AP-2,which is involved in protein sorting at theplasma membrane; related to the sigma subunitof the mammalian plasma membraneclathrin-associated protein (AP-2) complex 0.154490.56755&radic&radic S000000532FUS1YCL027WverifiedMembrane protein localized to the shmoo tip, required for cellfusion; expression regulated by matingpheromone; proposed to coordinate signaling,fusion, and polarization events required forfusion; potential Cdc28p substrate 0.152820.5636&radic&radic&radic S000004417TUS1YLR425WverifiedGuanine nucleotide exchange factor (GEF) that functions tomodulate Rho1p activity as part of the cellintegrity signaling pathway; multicopysuppressor of tor2 mutation and ypk1 ypk2double mutation; potential Cdc28p substrate 0.151830.56134 S000002175CDC7YDL017WverifiedDDK (Dbf4-dependent kinase) catalytic subunit required forfiring origins and replication fork progressionin mitosis through phosphorylation of Mcm2-7pcomplexes and Cdc45p; kinase activitycorrelates with cyclical DBF4 expression 0.148830.55537&radic&radic S000005233PCL1YNL289WverifiedPho85 cyclin of the Pcl1,2-like subfamily, involved in entryinto the mitotic cell cycle and regulation ofmorphogenesis, localizes to sites of polarizedcell growth 0.147970.55282&radic&radic S000000049FLC2YAL053WverifiedPutative FAD transporter; required for uptake of FAD intoendoplasmic reticulum; involved in cell wallmaintenance 0.146740.54976&radic&radic&radic S000005034RHO2YNL090WverifiedNon-essential small GTPase of the Rho/Rac subfamily of Ras-likeproteins, involved in the establishment of cellpolarity and in microtubule assembly 0.146720.54976&radic&radic S000005446ADH1YOL086CverifiedAlcohol dehydrogenase, fermentative isozyme active as homo- orheterotetramers; required for the reduction ofacetaldehyde to ethanol, the last step in theglycolytic pathway 0.14330.54488&radic S000002659PAM1YDR251WverifiedEssential protein of unknown function; exhibits variableexpression during colony morphogenesis;overexpression permits survival without proteinphosphatase 2A, inhibits growth, and induces afilamentous phenotype &radic0.142520.54373&radic&radic&radic&radic&radic&radic S000003851JSN1YJR091CverifiedMember of the Puf family of RNA-binding proteins, interactswith mRNAs encoding membrane-associatedproteins; overexpression suppresses a tub2-150mutation and causes increased sensitivity tobenomyl in wild-type cells 0.141180.54047&radic S000004305SPH1YLR313CverifiedProtein involved in shmoo formation and bipolar bud siteselection; homologous to Spa2p, localizes tosites of polarized growth in a cell cycledependent- and Spa2p-dependent manner,interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p &radic0.139460.53718&radic&radic&radic S000004284GSP1YLR293CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; regulated by Prp20p,Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p andKap95p; yeast Gsp2p homolog 0.139240.53584&radic&radic&radic S000004354STE11YLR362WverifiedSignal transducing MEK kinase involved in pheromone responseand pseudohyphal/invasive growth pathways whereit phosphorylates Ste7p, and the highosmolarity response pathway, viaphosphorylation of Pbs2p; regulated by Ste20pand Ste50p 0.137170.53271&radic&radic&radic S000000364CDC28YBR160WverifiedCatalytic subunit of the main cell cycle cyclin-dependentkinase (CDK); alternately associates with G1cyclins (CLNs) and G2/M cyclins (CLBs) whichdirect the CDK to specific substrates 0.136330.5308&radic&radic S000002305LDB17YDL146WverifiedProtein of unknown function; GFP-fusion protein localizes tothe cell periphery, cytoplasm, bud, and budneck; null mutant shows a reduced affinity forthe alcian blue dye suggesting a decreased netnegative charge of the cell surface &radic0.135480.52949&radic&radic&radic&radic S000001856YPT1YFL038CverifiedRas-like small GTPase, involved in the ER-to-Golgi step of thesecretory pathway; complex formation with theRab escort protein Mrs6p is required forprenylation of Ypt1p by proteingeranylgeranyltransferase type II (Bet2p-Bet4p) 0.135450.52941&radic&radic&radic S000001756NAP1YKR048CverifiedProtein that interacts with mitotic cyclin Clb2p; required forthe regulation of microtubule dynamics duringmitosis; controls bud morphogenesis; involvedin the transport of H2A and H2B histones to thenucleus 0.131320.51968&radic&radic&radic&radic&radic S000004710YPK2YMR104CverifiedProtein kinase with similarityto serine/threonine proteinkinase Ypk1p; functionally redundant with YPK1at the genetic level; participates in asignaling pathway required for optimal cellwall integrity; homolog of mammalian kinase SGK 0.130690.51817&radic&radic S000006076KIP2YPL155CverifiedKinesin-related motor protein involved in mitotic spindlepositioning, stabilizes microtubules bytargeting Bik1p to the plus end; Kip2p levelsare controlled during the cell cycle 0.130340.51705&radic&radic&radic S000000872RNR1YER070WverifiedRibonucleotide-diphosphate reductase (RNR), large subunit; theRNR complex catalyzes the rate-limiting step indNTP synthesis and is regulated by DNAreplication and DNA damage checkpoint pathwaysvia localization of the small subunits 0.129420.51532&radic&radic&radic S000000913SWI4YER111CverifiedDNA binding component of the SBF complex (Swi4p-Swi6p), atranscriptional activator that in concert withMBF (Mbp1-Swi6p) regulates late G1-specifictranscription of targets including cyclins andgenes required for DNA synthesis and repair 0.127730.51196&radic&radic S000001609YPK1YKL126WverifiedSerine/threonine protein kinase required for receptor-mediatedendocytosis; involved in sphingolipid-mediatedand cell integrity signaling pathways;localized to the bud neck, cytosol and plasmamembrane; homolog of mammalian kinase SGK &radic0.127280.51151&radic&radic&radic&radic S000000112FUS3YBL016WverifiedMitogen-activated protein kinase involved in mating pheromoneresponse; activated by phosphorylation bySte7p; provides specificity during the matingvs. filamentous growth response byphosphorylating transcriptional and cytoplasmictargets 0.127230.5115&radic&radic&radic&radic S000002553SWI5YDR146CverifiedTranscription factor that activates transcription of genesexpressed at the M/G1 phase boundary and in G1phase; localization to the nucleus occursduring G1 and appears to be regulated byphosphorylation by Cdc28p kinase 0.127160.51126&radic&radic&radic&radic S000003084CDC20YGL116WverifiedCell-cycle regulated activator of anaphase-promotingcomplex/cyclosome (APC/C), which is requiredfor metaphase/anaphase transition; directsubiquitination of mitotic cyclins, Pds1p, andother anaphase inhibitors; potential Cdc28psubstrate 0.126770.51077&radic&radic S000001445PAN1YIR006CverifiedPart of actin cytoskeleton-regulatory complexPan1p-Sla1p-End3p, associates with actinpatches on the cell cortex; promotesprotein-protein interactions essential forendocytosis; previously thought to be a subunitof poly(A) ribonuclease 0.125140.50786 S000002557NUM1YDR150WverifiedProtein required for nuclear migration, localizes to the mothercell cortex and the bud tip; may mediateinteractions of dynein and cytoplasmicmicrotubules with the cell cortex 0.124910.50774&radic S000004239YEF3YLR249WverifiedTranslational elongation factor, stimulates the binding ofaminoacyl-tRNA (AA-tRNA) to ribosomes byreleasing EF-1 alpha from the ribosomalcomplex; contains two ABC cassettes; binds andhydrolyses ATP 0.123970.50505&radic&radic&radic&radic&radic S000004845FUS2YMR232WverifiedCytoplasmic protein localized to the shmoo tip; required forthe alignment of parental nuclei before nuclearfusion during mating 0.12240.50076&radic&radic&radic S000000818BIM1YER016WverifiedMicrotubule-binding protein that together with Kar9p makes upthe cortical microtubule capture site anddelays the exit from mitosis when the spindleis oriented abnormally 0.119510.49386&radic S000003163GCN1YGL195WverifiedPositive regulator of the Gcn2p kinase activity, forms acomplex with Gcn20p; proposed to stimulateGcn2p activation by an uncharged tRNA 0.119290.49376&radic&radic S000005707LAS17YOR181WverifiedActin assembly factor, activates the Arp2/3 protein complexthat nucleates branched actin filaments;localizes with the Arp2/3 complex to actinpatches; homolog of the human Wiskott-Aldrichsyndrome protein (WASP) 0.116870.48698 S000004926NIP1YMR309CverifiedSubunit of the eukaryotic translation initiation factor 3(eIF3), involved in the assembly ofpreinitiation complex and start codon selection 0.114930.48228&radic&radic&radic&radic&radic S000004121ACE2YLR131CverifiedTranscription factor that activates expression of earlyG1-specific genes, localizes to daughter cellnuclei after cytokinesis and delays G1progression in daughters, localization isregulated by phosphorylation; potential Cdc28psubstrate 0.114460.48125&radic&radic&radic&radic S000001912YFR016CVerifiedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene 0.113280.47812&radic&radic&radic&radic S000001072SLT2YHR030CverifiedSerine/threonine MAP kinase involved in regulating themaintenance of cell wall integrity andprogression through the cell cycle; regulatedby the PKC1-mediated signaling pathway 0.112450.476&radic&radic&radic&radic S000003384RSR1YGR152CverifiedGTP-binding protein of the ras superfamily required for budsite selection, morphological changes inresponse to mating pheromone, and efficientcell fusion; localized to the plasma membrane;significantly similar to mammalian Rap GTPases &radic0.111920.47464&radic&radic&radic&radic&radic S000004349RSC2YLR357WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; involvedin telomere maintenance 0.111790.47409&radic&radic&radic&radic S000002787RGA2YDR379WverifiedGTPase-activating protein for the polarity-establishmentprotein Cdc42p; implicated in control of septinorganization, pheromone response, and haploidinvasive growth 0.111620.47409&radic&radic&radic&radic&radic S000002796RVS167YDR388WverifiedActin-associated protein, subunit of a complex(Rvs161p-Rvs167p) involved in regulation ofactin cytoskeleton, endocytosis, and viabilityfollowing starvation or osmotic stress; homologof mammalian amphiphysin 0.111120.47287&radic&radic S000001195SPO12YHR152WverifiedNucleolar protein of unknown function, positive regulator ofexit from mitosis; involved in regulating therelease of Cdc14p from the nucleolus in earlyanaphase; proposed to play similar role inmeiosis 0.110390.47123&radic&radic S000004180MMR1YLR190WverifiedPhosphorylated protein of the mitochondrial outer membrane,localizes only to mitochondria of the bud;interacts with Myo2p to mediate mitochondrialdistribution to buds; mRNA is targeted to thebud via the transport system involving She2p &radic0.110010.47035&radic&radic&radic&radic S000003557BBC1YJL020CverifiedProtein possibly involved in assembly of actin patches;interacts with an actin assembly factor Las17pand with the SH3 domains of Type I myosinsMyo3p and Myo5p; localized predominantly tocortical actin patches 0.109720.46936 S000004812CLN1YMR199WverifiedG1 cyclin involved in regulation of the cell cycle; activatesCdc28p kinase to promote the G1 to S phasetransition; late G1 specific expression dependson transcription factor complexes, MBF(Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) 0.108440.46692&radic&radic&radic&radic&radic&radic S000003700TPK1YJL164CverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; inhibits filamentous growth 0.10830.46662&radic&radic&radic&radic&radic S000000143EDE1YBL047CverifiedKey endocytic protein involved in a network of interactionswith other endocytic proteins, binds membranesin a ubiquitin-dependent manner, may also bindubiquitinated membrane-associated proteins &radic0.107690.46517&radic&radic&radic&radic S000004924GAS1YMR307WverifiedBeta-1.3-glucanosyltransferase, required for cell wallassembly; localizes to the cell surface via aglycosylphosphatidylinositol (GPI) anchor 0.107770.46517&radic S000001367SLM1YIL105CverifiedPhosphoinositide PI4,5P(2) binding protein, forms a complexwith Slm2p; acts downstream of Mss4p in apathway regulating actin cytoskeletonorganization in response to stress; subunit ofand phosphorylated by the TORC2 complex 0.106560.46191 S000004252YPT6YLR262CverifiedGTPase, Ras-like GTP binding protein involved in the secretorypathway, required for fusion ofendosome-derived vesicles with the late Golgi,maturation of the vacuolar carboxypeptidase Y;has similarity to the human GTPase, Rab6 0.106220.46137&radic&radic&radic&radic&radic S000005042RAS2YNL098CverifiedGTP-binding protein that regulates the nitrogen starvationresponse, sporulation, and filamentous growth;farnesylation and palmitoylation required foractivity and localization to plasma membrane;homolog of mammalian Ras proto-oncogenes 0.105990.46006&radic&radic S000005783CDC31YOR257WverifiedComponent of the spindle pole body (SPB) half-bridge, requiredfor SPB duplication in mitosis and meiosis II;homolog of mammalian centrin; interacts withKar1p 0.103810.45438&radic&radic S000005473SKM1YOL113WverifiedMember of the PAK family of serine/threonine protein kinaseswith similarity to Ste20p and Cla4p; proposedto be a downstream effector of Cdc42p duringpolarized growth 0.10290.45234&radic S000003147TOS3YGL179CverifiedProtein kinase, related to and functionally redundant withElm1p and Sak1p for the phosphorylation andactivation of Snf1p; functionally orthologousto LKB1, a mammalian kinase associated withPeutz-Jeghers cancer-susceptibility syndrome 0.101520.44907 S000003730CDC6YJL194WverifiedEssential ATP-binding protein required for DNA replication,component of the pre-replicative complex(pre-RC) which requires ORC to associate withchromatin and is in turn required for Mcm2-7pDNA association; homologous to S. pombe Cdc18p 0.100830.44709&radic&radic&radic&radic S000006369RHO1YPR165WverifiedGTP-binding protein of the rho subfamily of Ras-like proteins,involved in establishment of cell polarity;regulates protein kinase C (Pkc1p) and the cellwall synthesizing enzyme 1,3-beta-glucansynthase (Fks1p and Gsc2p) &radic0.099740.44343&radic&radic S000003214GUS1YGL245WverifiedGlutamyl-tRNA synthetase (GluRS), forms a complex withmethionyl-tRNA synthetase (Mes1p) and Arc1p;complex formation increases the catalyticefficiency of both tRNA synthetases and ensurestheir correct localization to the cytoplasm 0.098560.44018&radic&radic&radic S000000069BUD14YAR014CverifiedProtein involved in bud-site selection, Bud14p-Glc7p complexfunctions as a cortical regulator of dynein;diploid mutants display a random buddingpattern instead of the wild-type bipolarpattern &radic0.097790.43789&radic&radic&radic&radic&radic S000005894SCP1YOR367WverifiedComponent of yeast cortical actin cytoskeleton, binds and crosslinks actin filaments; originally identified byits homology to calponin (contains acalponin-like repeat) but the Scp1p domainstructure is more similar to transgelin 0.0970.43631 S000005774YOR248WDubiousHypothetical protein 0.096280.43398&radic&radic&radic S000004219CDC42YLR229CverifiedSmall rho-like GTPase, essential for establishment andmaintenance of cell polarity; mutants havedefects in the organization of actin andseptins &radic0.095550.43197 S000000684ABP1YCR088WverifiedActin-binding protein of the cortical actin cytoskeleton,important for activation of the Arp2/3 complexthat plays a key role actin in cytoskeletonorganization 0.095110.43093 S000006124TPK2YPL203WverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; activates filamentous growth 0.095130.43093&radic&radic&radic S000000081SWH1YAR042WverifiedProtein similar to mammalian oxysterol-binding protein;contains ankyrin repeats; localizes to theGolgi and the nucleus-vacuole junction 0.094220.42805&radic&radic&radic S000004645ARG80YMR042WverifiedTranscription factor involved in regulation ofarginine-responsive genes; acts with Arg81p andArg82p 0.093830.42676&radic&radic&radic S000004334FKS1YLR342WverifiedCatalytic subunit of 1,3-beta-D-glucan synthase, functionallyredundant with alternate catalytic subunitGsc2p; binds to regulatory subunit Rho1p;involved in cell wall synthesis andmaintenance; localizes to sites of cell wallremodeling 0.093560.42549&radic&radic S000005124RHO5YNL180CverifiedNon-essential small GTPase of the Rho/Rac subfamily of Ras-likeproteins, likely involved in protein kinase C(Pkc1p)-dependent signal transduction pathwaythat controls cell integrity 0.093510.42549&radic&radic&radic S000005587CKA2YOR061WverifiedAlpha' catalytic subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA1, CKB1 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 0.090560.41568&radic&radic&radic&radic S000004577ZDS2YML109WverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; paralogof Zds1p 0.090110.41377&radic S000005773SRL1YOR247WverifiedMannoprotein that exhibits a tight association with the cellwall, required for cell wall stability in theabsence of GPI-anchored mannoproteins; has ahigh serine-threonine content; expression isinduced in cell wall mutants 0.090080.41377&radic&radic&radic S000002797SAC7YDR389WverifiedGTPase activating protein (GAP) for Rho1p, involved insignaling to the actin cytoskeleton, nullmutations suppress tor2 mutations andtemperature sensitive mutations in actin;potential Cdc28p substrate 0.089790.41299&radic&radic&radic&radic S000006073RRD2YPL152WverifiedActivator of the phosphotyrosyl phosphatase activity of PP2A;regulates G1 phase progression, theosmoresponse and microtubule dynamics;implicated in the spindle assembly check;subunit of the Tap42p-Pph21p-Rrd2p complex 0.089630.41236&radic&radic&radic&radic S000004324MID2YLR332WverifiedO-glycosylated plasma membrane protein that acts as a sensorfor cell wall integrity signaling and activatesthe pathway; interacts with Rom2p, a guaninenucleotide exchange factor for Rho1p, and withcell integrity pathway protein Zeo1p 0.089580.41236&radic S000001177YCK1YHR135CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck2p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck2p 0.089020.41091&radic&radic&radic S000005757MKK1YOR231WverifiedMitogen-activated kinase kinase involved in protein kinase Csignaling pathway that controls cell integrity;upon activation by Bck1p phosphorylatesdownstream target, Slt2p; functionallyredundant with Mkk2p 0.088730.4098&radic&radic&radic&radic S000004329VRP1YLR337CverifiedProline-rich actin-associated protein involved in cytoskeletalorganization and cytokinesis; related tomammalian Wiskott-Aldrich syndrome protein(WASP)-interacting protein (WIP) 0.088320.40858&radic&radic S000002789YRA1YDR381WverifiedNuclear protein that binds to RNA and to Mex67p, required forexport of poly(A)+ mRNA from the nucleus;member of the REF (RNA and export factorbinding proteins) family; another familymember, Yra2p, can substitute for Yra1pfunction 0.087970.40709&radic&radic&radic&radic&radic S000005627RAS1YOR101WverifiedGTPase involved in G-protein signaling in the adenylate cyclaseactivating pathway, plays a role in cellproliferation; localized to the plasmamembrane; homolog of mammalian RASproto-oncogenes 0.086430.40124&radic&radic S000004529TEM1YML064CverifiedGTP-binding protein of the ras superfamily involved intermination of M-phase; controls actomyosin andseptin dynamics during cytokinesis 0.086430.40124 S000002500DNF2YDR093WverifiedAminophospholipid translocase (flippase) that localizesprimarily to the plasma membrane; contributesto endocytosis, protein transport and cellpolarity; type 4 P-type ATPase 0.086340.40039&radic&radic&radic&radic&radic S000005952PHO85YPL031CverifiedCyclin-dependent kinase, with ten cyclin partners; involved inenvironmental stress response; inphosphate-rich conditions, Pho85p-Pho80pcomplex phosphorylates Pho4p which in turnrepresses PHO5 0.086220.40029&radic&radic&radic&radic S000003631BCK1YJL095WverifiedMitogen-activated protein (MAP) kinase kinase kinase acting inthe protein kinase C signaling pathway, whichcontrols cell integrity; upon activation byPkc1p phosphorylates downstream kinases Mkk1pand Mkk2p 0.085870.39928&radic S000004301CDC25YLR310CverifiedMembrane bound guanine nucleotide exchange factor (GEF orGDP-release factor); indirectly regulatesadenylate cyclase through activation of Ras1pand Ras2p by stimulating the exchange of GDPfor GTP; required for progression through G1 0.08520.39718&radic&radic&radic&radic S000001613SHE2YKL130CverifiedRNA-binding protein that binds specific mRNAs and interactswith She3p; part of the mRNA localizationmachinery that restricts accumulation ofcertain proteins to the bud 0.08510.39647&radic&radic&radic&radic&radic&radic S000003302ROM1YGR070WverifiedGDP/GTP exchange protein (GEP) for Rho1p; mutations aresynthetically lethal with mutations in rom2,which also encodes a GEP 0.084960.39604&radic&radic&radic S000001115OSH3YHR073WverifiedMember of an oxysterol-binding protein family with sevenmembers in S. cerevisiae; family members haveoverlapping, redundant functions in sterolmetabolism and collectively perform a functionessential for viability 0.08410.39335&radic&radic&radic&radic&radic S000001580YKL097CDubiousDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species 0.083930.3931&radic S000001385SIM1YIL123WverifiedProtein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that mayparticipate in DNA replication, promotercontains SCB regulation box at -300 bpindicating that expression may be cellcycle-regulated 0.082610.3884 S000000339CKS1YBR135WverifiedSubunit of the Cdc28 protein kinase, required for mitoticproteolysis, may also be involved in theproteolysis of the G1 cyclins 0.082350.38789&radic&radic S000000601SAT4YCR008WverifiedSer/Thr protein kinase involved in salt tolerance; funtions inregulation of Trk1p-Trk2p potassiumtransporter; partially redundant with Hal5p;has similarity to Npr1p 0.082040.38666&radic S000001649TPK3YKL166CverifiedSubunit of cAMP-dependent protein kinase, which contains Tpk1p,Tpk2p, Tpk3p, and regulatory subunit Bcy1p;promotes growth in response to nutrients;mediates induction of apoptosis due toactin-mediated hyperactivation of Ras signaling 0.081470.38518&radic&radic&radic&radic&radic S000006130IPL1YPL209CverifiedAurora kinase involved in regulating kinetochore-microtubuleattachments; helps maintain condensedchromosomes during anaphase and earlytelophase; associates with Sli15p, whichpromotes Ipl1p association with mitotic spindle 0.080320.38127&radic&radic&radic S000000072CDC15YAR019CverifiedProtein kinase of the Mitotic Exit Network that is localized tothe spindle pole bodies at late anaphase;promotes mitotic exit by directly switching onthe kinase activity of Dbf2p &radic0.079510.37691&radic&radic&radic&radic S000001047GPA1YHR005CverifiedGTP-binding alpha subunit of the heterotrimeric G protein thatcouples to pheromone receptors; negativelyregulates the mating pathway by sequesteringG(beta)gamma and by triggering an adaptiveresponse; activates Vps34p at the endosome 0.079230.37649&radic S000005679PDR5YOR153WverifiedShort-lived membrane ABC (ATP-binding cassette) transporter,actively exports various drugs, expressionregulated by Pdr1p; also involved in steroidtransport, cation resistance, and cellulardetoxification during exponential growth 0.078650.37391&radic&radic&radic&radic S000002492AFR1YDR085CverifiedAlpha-factor pheromone receptor regulator, negatively regulatespheromone receptor signaling; required fornormal mating projection (shmoo) formation;interacts with Cdc12p 0.078370.37388&radic&radic&radic&radic S000003664PBS2YJL128CverifiedMAP kinase kinase that plays a pivotal role in the osmosensingsignal-transduction pathway, activated undersevere osmotic stress 0.078030.37284&radic S000004406YLR414CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the bud and cytoplasm; Hog1p is required for transcriptional induction in response to cell wall damage; YLR414C is not an essential gene 0.078050.37284&radic&radic&radic S000005028END3YNL084CverifiedEH domain-containing protein involved in endocytosis, actincytoskeletal organization and cell wallmorphogenesis; forms a complex with Sla1p andPan1p 0.07720.36988 S000005759KIN4YOR233WverifiedKinase that acts by inhibiting the mitotic exit network (MEN)when the spindle position checkpoint isactivated; localized asymmetrically to mothercell cortex, spindle pole body and bud neck &radic0.076760.36838&radic&radic&radic&radic&radic S000003158CDC55YGL190CverifiedNon-essential regulatory subunit B of protein phosphatase 2A;has multiple roles in mitosis and proteinbiosynthesis; found in the nucleus of mostcells but also at the bud neck (large-buddedcells) and at the bud tip (small-budded cells) &radic0.075890.36515&radic S000001910CMK1YFR014CverifiedCalmodulin-dependent protein kinase; may play a role in stressresponse, many CA++/calmodulan dependentphosphorylation substrates demonstrated invitro, amino acid sequence similar to Cmk2p andmammalian Cam Kinase II 0.075770.36388&radic&radic&radic&radic S000004339KAP95YLR347CverifiedKaryopherin beta, forms a dimeric complex with Srp1p (Kap60p)that mediates nuclear import of cargo proteinsvia a nuclear localization signal (NLS),interacts with nucleoporins to guide transportacross the nuclear pore complex 0.075450.3635&radic&radic&radic S000006177CLN2YPL256CverifiedG1 cyclin involved in regulation of the cell cycle; activatesCdc28p kinase to promote the G1 to S phasetransition; late G1 specific expression dependson transcription factor complexes, MBF(Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) 0.075290.3632&radic&radic&radic&radic&radic S000004747RIM11YMR139WverifiedProtein kinase required for signal transduction during entryinto meiosis; promotes the formation of theIme1p-Ume6p complex by phosphorylating Ime1pand Ume6p; shares similarity with mammalianglycogen synthase kinase 3-beta 0.075160.36255&radic S000001058YSC84YHR016CverifiedProtein involved in the organization of the actin cytoskeleton;contains SH3 domain similar to Rvs167p 0.075160.36255 S000003472PFK1YGR240CverifiedAlpha subunit of heterooctameric phosphofructokinase involvedin glycolysis, indispensable for anaerobicgrowth, activated by fructose-2,6-bisphosphateand AMP, mutation inhibits glucose induction ofcell cycle-related genes 0.074590.3607&radic&radic&radic S000000927RSP5YER125WverifiedUbiquitin-protein ligase involved in ubiquitin-mediated proteindegradation; plays a role in heat shock element(HSE)-mediated gene expression andmultivesicular body sorting; contains a hect(homologous to E6-AP carboxyl terminus) domain 0.073270.35571 S000001855ACT1YFL039CverifiedActin, structural protein involved in cell polarization,endocytosis, and other cytoskeletal functions &radic0.073140.35527&radic&radic&radic S000002529KIN1YDR122WverifiedSerine/threonine protein kinase involved in regulation ofexocytosis; localizes to the cytoplasmic faceof the plasma membrane; closely related toKin2p 0.072980.35493&radic&radic&radic&radic S000001722YPT52YKR014CverifiedGTPase, similar to Ypt51p and Ypt53p and to mammalian rab5;required for vacuolar protein sorting andendocytosis 0.072950.35444&radic&radic&radic&radic&radic&radic S000006074RAD53YPL153CverifiedProtein kinase, required for cell-cycle arrest in response toDNA damage; activated by transautophosphorylation when interacting withhyperphosphorylated Rad9p; also interacts withARS1 and plays a role in initiation of DNAreplication 0.072680.35374&radic S000002259DUN1YDL101CverifiedCell-cycle checkpoint serine-threonine kinase required for DNAdamage-induced transcription of certain targetgenes, phosphorylation of Rad55p and Sml1p, andtransient G2/M arrest after DNA damage; alsoregulates postreplicative DNA repair 0.071790.34987&radic S000001529DCW1YKL046CverifiedPutative mannosidase, GPI-anchored membrane protein requiredfor cell wall biosynthesis in budformation;homologous to Dfg5p 0.071450.34859&radic&radic&radic&radic S000005376CMK2YOL016CverifiedCalmodulin-dependent protein kinase; may play a role in stressresponse, many CA++/calmodulan dependentphosphorylation substrates demonstrated invitro, amino acid sequence similar to Cmk1p andmammalian Cam Kinase II 0.071270.34841&radic S000002976PMA1YGL008CverifiedPlasma membrane H+-ATPase, pumps protons out of the cell; majorregulator of cytoplasmic pH and plasma membranepotential; part of the P2 subgroup ofcation-transporting ATPases 0.071220.3482&radic&radic&radic&radic&radic S000003485PUP2YGR253CverifiedAlpha subunit of the 20S proteasome involved inubiquitin-dependent catabolism; human homologis subunit zeta 0.070840.3461&radic&radic S000005237MSB3YNL293WverifiedGTPase-activating protein for Sec4p and several other RabGTPases, regulates exocytosis via its action onSec4p, also required for proper actinorganization; similar to Msb4p; both Msb3p andMsb4p localize to sites of polarized growth 0.071050.3461&radic S000001103GIC1YHR061CverifiedProtein of unknown function involved in initiation of buddingand cellular polarization, interacts withCdc42p via the Cdc42/Rac-interactive binding(CRIB) domain &radic0.070660.34592&radic&radic&radic&radic S000004910YMR295CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene 0.070340.34385&radic&radic S000000814PRE1YER012Wverified20S proteasome beta-type subunit; localizes to the nucleusthroughout the cell cycle 0.06880.33867&radic&radic S000002318STE7YDL159WverifiedSignal transducing MAP kinase kinase involved in pheromoneresponse, where it phosphorylates Fus3p, and inthe pseudohyphal/invasive growth pathway,through phosphorylation of Kss1p;phosphorylated by Ste11p, degraded by ubiquitinpathway 0.068270.33665&radic S000003246MSB2YGR014WverifiedMucin family member at the head of the Cdc42p- and MAPkinase-dependent filamentous growth signalingpathway; also functions as an osmosensor inparallel to the Sho1p-mediated pathway;potential Cdc28p substrate 0.068080.33575&radic&radic S000005963SSN3YPL042CverifiedCyclin-dependent protein kinase, component of RNA polymerase IIholoenzyme; involved in phosphorylation of theRNA polymerase II C-terminal domain; involvedin glucose repression 0.067670.33439&radic S000005842SFG1YOR315WverifiedNuclear protein, putative transcription factor required forgrowth of superficial pseudohyphae (which donot invade the agar substrate) but not forinvasive pseudohyphal growth; may act togetherwith Phd1p; potential Cdc28p substrate 0.067560.33408&radic&radic&radic&radic S000005027SAL1YNL083WverifiedProbable transporter, member of the Ca2+-binding subfamily ofthe mitochondrial carrier family, with twoEF-hand motifs; Pet9p and Sal1p have anoverlapping function critical for viability;polymorphic in different S. cerevisiae strains 0.06750.33406 S000002781FRQ1YDR373WverifiedN-myristoylated calcium-binding protein that may have a role inintracellular signaling through its regulationof the phosphatidylinositol 4-kinase Pik1p;member of the recoverin/frequenin branch of theEF-hand superfamily 0.066950.33171&radic&radic&radic S000002874PKH3YDR466WverifiedProtein kinase with similarity to mammalianphosphoinositide-dependent kinase 1 (PDK1) andyeast Pkh1p and Pkh2p, two redundant upstreamactivators of Pkc1p; identified as a multicopysuppressor of a pkh1 pkh2 double mutant 0.066250.32945 S000004795SSO2YMR183CverifiedPlasma membrane t-SNARE involved in fusion of secretoryvesicles at the plasma membrane; syntaxinhomolog that is functionally redundant withSso1p 0.066140.32894&radic&radic&radic&radic S000003424TDH3YGR192CverifiedGlyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involvedin glycolysis and gluconeogenesis; tetramerthat catalyzes the reaction ofglyceraldehyde-3-phosphate to 1,3bis-phosphoglycerate; detected in the cytoplasmand cell-wall 0.065860.32794&radic&radic&radic&radic&radic S000004460YPT7YML001WverifiedGTPase; GTP-binding protein of the rab family; required forhomotypic fusion event in vacuole inheritance,for endosome-endosome fusion, similar tomammalian Rab7 0.06450.32252&radic&radic&radic&radic&radic S000006365SGV1YPR161CverifiedCyclin (Bur2p)-dependent protein kinase that functions intranscriptional regulation; phosphorylates thecarboxy-terminal domain of Rpo21p, which is thelargest subunit of RNA polymerase II; regulatedby Cak1p 0.064470.32252&radic S000002569NBP2YDR162CverifiedProtein involved in the HOG (high osmolarity glycerol) pathway,negatively regulates Hog1p by recruitment ofphosphatase Ptc1p the Pbs2p-Hog1p complex,found in the nucleus and cytoplasm, contains anSH3 domain that binds Pbs2p 0.064450.32217&radic&radic&radic&radic S000004992SLM2YNL047CverifiedPhosphoinositide PI4,5P(2) binding protein, forms a complexwith Slm1p; acts downstream of Mss4p in apathway regulating actin cytoskeletonorganization in response to stress; subunit ofand phosphorylated by the TORC2 complex 0.064230.32132 S000003677YAK1YJL141CverifiedSerine-threonine protein kinase that is part of aglucose-sensing system involved in growthcontrol in response to glucose availability;translocates from the cytoplasm to the nucleusand phosphorylates Pop2p in response to aglucose signal 0.064020.32064 S000005314SSK2YNR031CverifiedMAP kinase kinase kinase of the HOG1 mitogen-activatedsignaling pathway; interacts with Ssk1p,leading to autophosphorylation and activationof Ssk2p which phosphorylates Pbs2p; alsomediates actin cytoskeleton recovery fromosmotic stress &radic0.063980.32044&radic S000001564TEF4YKL081WverifiedTranslation elongation factor EF-1 gamma 0.063310.31723&radic&radic&radic S000001665FAS1YKL182WverifiedBeta subunit of fatty acid synthetase, which catalyzes thesynthesis of long-chain saturated fatty acids;contains acetyltransacylase, dehydratase, enoylreductase, malonyl transacylase, and palmitoyltransacylase activities 0.06250.31195&radic&radic&radic S000001124KSP1YHR082CverifiedNonessential putative serine/threonine protein kinase ofunknown cellular role; overproduction causesallele-specific suppression of the prp20-10mutation 0.062330.31195&radic&radic&radic&radic S000006062YPL141CUncharacterizedHypothetical protein 0.062580.31195&radic&radic&radic&radic S000006358PIN3YPR154WverifiedProtein that induces appearance of [PIN+] prion whenoverproduced 0.062470.31195&radic&radic&radic&radic&radic S000003693FAR1YJL157CverifiedCyclin-dependent kinase inhibitor that mediates cell cyclearrest in response to pheromone; also forms acomplex with Cdc24p, Ste4p, and Ste18p that mayspecify the direction of polarized growthduring mating; potential Cdc28p substrate 0.061880.30983&radic&radic&radic&radic&radic S000006176BBP1YPL255WverifiedProtein required for the spindle pole body (SPB) duplication,localized at the central plaque periphery;forms a complex with a nuclear envelope proteinMps2p and SPB components Spc29p and Kar1p;required for mitotic functions of Cdc5p 0.061360.30787&radic&radic S000003367PRE9YGR135Wverified20S proteasome beta-type subunit; the only nonessential 20Ssubunit 0.060850.30593&radic&radic&radic S000004965ARK1YNL020CverifiedSerine/threonine protein kinase involved in regulation of thecortical actin cytoskeleton; involved incontrol of endocytosis 0.060620.30477 S000002655VHS1YDR247WverifiedCytoplasmic serine/threonine protein kinase; identified as ahigh-copy suppressor of the synthetic lethalityof a sis2 sit4 double mutant, suggesting a rolein G1/S phase progression; homolog of Sks1p 0.059690.30081&radic&radic&radic&radic S000002989ALK1YGL021WverifiedProtein kinase; accumulation and phosphorylation are periodicduring the cell cycle; phosphorylated inresponse to DNA damage; contains characteristicmotifs for degradation via the APC pathway;similar to Alk2p and to mammalian haspins 0.058980.29739&radic&radic&radic S000003135PMR1YGL167CverifiedHigh affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ andMn2+ transport into Golgi; involved in Ca2+dependent protein sorting and processing;mutations in human homolog ATP2C1 causeacantholytic skin condition Hailey-Haileydisease 0.058820.29713 S000005050INP52YNL106CverifiedPhosphatidylinositol 4,5-bisphosphate 5-phosphatase,synaptojanin-like protein with an N-terminalSac1 domain, plays a role in endocytosis;hyperosmotic stress causes translocation toactin patches 0.058750.29669 S000002885SNF1YDR477WverifiedAMP-activated serine/threonine protein kinase found in acomplex containing Snf4p and members of theSip1p/Sip2p/Gal83p family; required fortranscription of glucose-repressed genes,thermotolerance, sporulation, and peroxisomebiogenesis 0.058610.29628&radic S000003513YOR1YGR281WverifiedPlasma membrane transporter of the ATP-binding cassette (ABC)family, mediates export of many differentorganic anions including oligomycin 0.058470.29518&radic&radic S000004827SCJ1YMR214WverifiedOne of several homologs of bacterial chaperone DnaJ, located inthe ER lumen where it cooperates with Kar2p tomediate maturation of proteins 0.058490.29518&radic&radic&radic S000005900NUD1YOR373WverifiedComponent of the spindle pole body outer plaque, required forexit from mitosis 0.057480.29095&radic&radic S000006071YPL150WUncharacterizedHypothetical protein 0.057370.29051&radic&radic&radic S000001865CAK1YFL029CverifiedCyclin-dependent kinase-activating kinase required for passagethrough the cell cycle, phosphorylates andactivates Cdc28p; nucleotide-binding pocketdiffers significantly from those of most otherprotein kinases 0.057170.28952&radic S000000912KAP123YER110CverifiedKaryopherin beta, mediates nuclear import of ribosomal proteinsprior to assembly into ribosomes and import ofhistones H3 and H4; localizes to the nuclearpore, nucleus, and cytoplasm; exhibits geneticinteractions with RAI1 0.056610.28714&radic&radic&radic S000000071KIN3YAR018CverifiedNonessential protein kinase with unknown cellular role 0.05650.28675&radic S000001357PRK1YIL095WverifiedProtein serine/threonine kinase; regulates the organization andfunction of the actin cytoskeleton through thephosphorylation of the Pan1p-Sla1p-End3pprotein complex 0.056430.28646&radic&radic&radic&radic S000004773INP2YMR163CverifiedPeroxisome-specific receptor important for peroxisomeinheritance; co-fractionates with peroxisomemembranes and co-localizes with peroxisomes invivo; physically interacts with the myosin Vmotor Myo2p; INP2 is not an essential gene 0.056450.28646&radic S000001248SCH9YHR205WverifiedProtein kinase that regulates signal transduction activity andG1 progression, controls cAPK activity,required for nitrogen activation of the FGMpathway, involved in life span regulation,homologous to mammalian Akt/PKB 0.056190.28567&radic S000004886ZDS1YMR273CverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; has arole in localization of Bcy1p, a regulatorysubunit of protein kinase A; implicated in mRNAnuclear export &radic0.056110.28554&radic&radic&radic&radic S000003178YPT32YGL210WverifiedGTPase of the Ypt/Rab family, very similar to Ypt31p; involvedin the exocytic pathway; mediates intra-Golgitraffic or the budding of post-Golgi vesiclesfrom the trans-Golgi 0.055970.28477&radic&radic S000002508ARX1YDR101CverifiedShuttling pre-60S factor; involved in the biogenesis ofribosomal large subunit biogenesis; interactsdirectly with Alb1; responsible for Tif6recycling defects in absence of Rei1;associated with the ribosomal export complex 0.056010.28477&radic&radic S000000537STE50YCL032WverifiedProtein involved in mating response, invasive/filamentousgrowth, and osmotolerance, acts as an adaptorthat links G protein-associated Cdc42p-Ste20pcomplex to the effector Ste11p to modulatesignal transduction 0.055840.28413&radic&radic&radic S000003966FPS1YLL043WverifiedPlasma membrane glycerol channel, member of the major intrinsicprotein (MIP) family of channel proteins;involved in efflux of glycerol and in uptake ofthe trivalent metalloids arsenite andantimonite 0.055710.28352&radic&radic S000004179ATG26YLR189CverifiedUDP-glucose:sterol glucosyltransferase, conserved enzymeinvolved in synthesis of sterol glucosidemembrane lipids, involved in autophagy 0.05560.28224&radic&radic&radic&radic&radic S000006061MKK2YPL140CverifiedMitogen-activated kinase kinase involved in protein kinase Csignaling pathway that controls cell integrity;upon activation by Bck1p phosphorylatesdownstream target, Slt2p; functionallyredundant with Mkk1p 0.055540.28224&radic&radic&radic&radic&radic S000000362AMN1YBR158WverifiedProtein required for daughter cell separation, multiple mitoticcheckpoints, and chromosome stability; contains12 degenerate leucine-rich repeat motifs;expression is induced by the Mitotic ExitNetwork (MEN) 0.055080.28102&radic&radic S000003461SMI1YGR229CverifiedProtein involved in the regulation of cell wall synthesis;proposed to be involved in coordinating cellcycle progression with cell wall integrity 0.054630.27945&radic&radic&radic&radic S000005251MCK1YNL307CverifiedProtein serine/threonine/tyrosine (dual-specificity) kinaseinvolved in control of chromosome segregationand in regulating entry into meiosis; relatedto mammalian glycogen synthase kinases of theGSK-3 family 0.054520.27892&radic&radic&radic S000003393RTS3YGR161CuncharacterizedPutative component of the protein phosphatase type 2A complex 0.05420.27763&radic&radic&radic&radic&radic S000005876CIN1YOR349WverifiedTubulin folding factor D involved in beta-tubulin (Tub2p)folding; isolated as mutant with increasedchromosome loss and sensitivity to benomyl 0.053970.27676&radic&radic&radic S000005583SGT1YOR057WverifiedProbable cochaperone, regulates activity of Cyr1p (adenylylcyclase); involved in assembly of thekinetochore complex, associates with the SCF(Skp1p/Cdc53p/F box protein) ubiquitin ligasecomplex 0.053890.27649&radic&radic&radic&radic S000003162HOS2YGL194CverifiedHistone deacetylase required for gene activation via specificdeacetylation of lysines in H3 and H4 histonetails; subunit of the Set3 complex, ameiotic-specific repressor of sporulationspecific genes that contains deacetylaseactivity 0.053270.2742&radic&radic&radic&radic S000000232YBR028CUncharacterizedHypothetical protein 0.053160.27388&radic S000000241SCO1YBR037CverifiedCopper-binding protein of the mitochondrial inner membrane,required for cytochrome c oxidase activity andrespiration; may function to deliver copper tocytochrome c oxidase; has similarity tothioredoxins 0.053040.27337 S000002691GCN2YDR283CverifiedProtein kinase, phosphorylates the alpha-subunit of translationinitiation factor eIF2 (Sui2p) in response tostarvation; activated by uncharged tRNAs andthe Gcn1p-Gcn20p complex 0.052970.27309&radic&radic&radic&radic&radic S000000881YER079WUncharacterizedHypothetical protein 0.052530.27135&radic&radic&radic S000000845SAH1YER043CverifiedS-adenosyl-L-homocysteine hydrolase, catabolizesS-adenosyl-L-homocysteine which is formed afterdonation of the activated methyl group ofS-adenosyl-L-methionine (AdoMet) to an acceptor 0.052470.27128&radic&radic S000001297CKA1YIL035CverifiedAlpha catalytic subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA2, CKB1 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 0.052310.2705&radic&radic&radic&radic&radic S000004752YMR144WUncharacterizedPutative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene 0.051960.26876&radic&radic&radic S000001106SSZ1YHR064CverifiedHsp70 protein that interacts with Zuo1p (a DnaJ homolog) toform a ribosome-associated complex that bindsthe ribosome via the Zuo1p subunit; alsoinvolved in pleiotropic drug resistance viasequential activation of PDR1 and PDR5; bindsATP 0.051910.26876&radic S000004528RPS1BYML063WverifiedRibosomal protein 10 (rp10) of the small (40S) subunit; nearlyidentical to Rps1Ap and has similarity to ratS3a ribosomal protein 0.051550.26604&radic S000003340CLB1YGR108WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 0.051480.26604&radic&radic&radic&radic&radic S000000931SAK1YER129WverifiedUpstream kinase for the SNF1 complex; partially redundantfunction with Elm1p and Tos3p; members of thisfamily of kinases have functional orthologywith LKB1, a mammalian kinase associated withPeutz-Jeghers cancer-susceptibility syndrome 0.051320.26604&radic S000000281SLM4YBR077CverifiedComponent of the EGO complex, which is involved in theregulation of microautophagy, and of the GSEcomplex, which is required for proper sortingof amino acid permease Gap1p; gene exhibitssynthetic genetic interaction with MSS4 0.051490.26604 S000004595ERG13YML126Cverified3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzesthe formation of HMG-CoA from acetyl-CoA andacetoacetyl-CoA; involved in the second step inmevalonate biosynthesis 0.051160.26529&radic S000003332MDR1YGR100WverifiedCytoplasmic GTPase-activating protein for Ypt/Rab transportGTPases Ypt6p, Ypt31p and Sec4p; involved inrecycling of internalized proteins andregulation of Golgi secretory function 0.050920.26439&radic&radic&radic&radic S000001739SPO14YKR031CverifiedPhospholipase D, catalyzes the hydrolysis ofphosphatidylcholine, producing choline andphosphatidic acid; involved inSec14p-independent secretion; required formeiosis and spore formation; differentlyregulated in secretion and meiosis 0.050830.26395&radic&radic&radic&radic S000003899IML1YJR138WverifiedProtein of unknown function, green fluorescent protein(GFP)-fusion protein localizes to the vacuolarmembrane 0.050570.26285 S000000200YBL104CUncharacterizedHypothetical protein 0.050640.26285&radic S000001737SET3YKR029CverifiedDefining member of the SET3 histone deacetylase complex whichis a meiosis-specific repressor of sporulationgenes; necessary for efficient transcription byRNAPII; one of two yeast proteins that containsboth SET and PHD domains 0.050250.26185&radic S000006307PRE2YPR103Wverified20S proteasome beta-type subunit, responsible for thechymotryptic activity of the proteasome 0.049890.26044&radic&radic S000005664RUP1YOR138CverifiedProtein involved in regulation of Rsp5p, which is an essentialHECT ubiquitin ligase; required for binding ofRsp5p to Ubp2p; contains an UBA domain 0.04980.26027&radic&radic&radic&radic S000001173YHR131CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.049370.2587&radic S000005540RTS1YOR014WverifiedB-type regulatory subunit of protein phosphatase 2A (PP2A);homolog of the mammalian B' subunit of PP2A &radic0.049230.25809&radic&radic&radic&radic&radic S000002183YDL025CUncharacterizedPutative protein kinase, potentially phosphorylated by Cdc28p; YDL025C is not an essential gene 0.04880.25595&radic S000004103HOG1YLR113WverifiedMitogen-activated protein kinase involved in osmoregulation viathree independent osmosensors; mediates therecruitment and activation of RNA Pol II atHot1p-dependent promoters; localizationregulated by Ptp2p and Ptp3p 0.048460.25535&radic&radic&radic&radic S000004563VPS9YML097CverifiedA guanine nucleotide exchange factor involved invesicle-mediated vacuolar protein transport;specifically stimulates the intrinsic guaninenucleotide exchange activity of Vps21p/Rab5:similar to mammalian ras inhibitors; bindsubiquitin 0.048260.2542&radic S000000307SIF2YBR103WverifiedWD40 repeat-containing subunit of the Set3C histone deacetylasecomplex, which represses early/middlesporulation genes; antagonizes telomericsilencing; binds specifically to the Sir4pN-terminus 0.04790.25331&radic S000005201SIP3YNL257CverifiedProtein that activates transcription through interaction withDNA-bound Snf1p, C-terminal region has aputative leucine zipper motif; potential Cdc28psubstrate 0.047850.25281&radic&radic S000005660BAG7YOR134WverifiedRho GTPase activating protein (RhoGAP), stimulates theintrinsic GTPase activity of Rho1p, which playsa role in actin cytoskeleton organization andcontrol of cell wall synthesis; structurallyand functionally related to Sac7p 0.047590.25221&radic S000001225YHR182WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm 0.047460.25162&radic&radic S000001719YKR011CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus 0.047180.25067&radic&radic S000001374HOS4YIL112WuncharacterizedSubunit of the Set3 complex, which is a meiotic-specificrepressor of sporulation specific genes thatcontains deacetylase activity; potential Cdc28psubstrate 0.046930.2499&radic S000001064YHR022CUncharacterizedPutative protein of unknown function; YHR022C is not an essential gene. 0.046910.2499&radic&radic&radic S000000629SNT1YCR033WverifiedSubunit of the Set3C deacetylase complex that interactsdirectly with the Set3C subunit, Sif2p;putative DNA-binding protein 0.046710.24897&radic S000004613HXT2YMR011WverifiedHigh-affinity glucose transporter of the major facilitatorsuperfamily, expression is induced by lowlevels of glucose and repressed by high levelsof glucose 0.046650.24887&radic&radic&radic&radic S000002440MRH1YDR033WverifiedProtein that localizes primarily to the plasma membrane, alsofound at the nuclear envelope; has similarityto Hsp30p and Yro2p, which are induced duringheat shock 0.046310.24753&radic S000003423HIP1YGR191WverifiedHigh-affinity histidine permease, also involved in thetransport of manganese ions 0.046090.2466&radic&radic&radic&radic S000003165MDS3YGL197WverifiedProtein with an N-terminal kelch-like domain, putative negativeregulator of early meiotic gene expression;required, with Pmd1p, for growth under alkalineconditions 0.046130.2466&radic&radic&radic S000001956CWP2YKL096W-AverifiedCovalently linked cell wall mannoprotein, major constituent ofthe cell wall; plays a role in stabilizing thecell wall; involved in low pH resistance;precursor is GPI-anchored 0.046070.2466 S000000994HSE1YHL002WverifiedSubunit of the endosomal Vps27p-Hse1p complex required forsorting of ubiquitinated membrane proteins intointralumenal vesicles prior to vacuolardegradation, as well as for recycling of Golgiproteins and formation of lumenal membranes 0.045620.24492 S000002686YDR278CDubiousHypothetical protein 0.045650.24492&radic&radic&radic&radic S000000478CHK1YBR274WverifiedDNA damage checkpoint effector kinase, mediates mitotic cellcycle arrest via phosphorylation of Pds1p;phosphorylated by checkpoint signal transducerkinase Mec1p; homolog of S. pombe and mammalianChk1 checkpoint kinase 0.04560.24446&radic S000003045HNM1YGL077CverifiedCholine transporter (permease) that also controls the uptake ofnitrogen mustard; expression is co-regulatedwith phospholipid biosynthetic genes andnegatively regulated by choline andmyo-inositol 0.045530.24446&radic&radic&radic&radic S000000468YPT10YBR264CverifiedGTP binding protein that contains the PEST signal sequencespecific for proteolytic enzymes; may beinvolved in vesicular transport; overexpressionleads to accumulation of Golgi-like cisternaewith budding vesicles 0.045320.24329&radic&radic S000005248YPT11YNL304WverifiedRab-type small GTPase that interacts with the C-terminal taildomain of Myo2p to mediate distribution ofmitochondria to daughter cells &radic0.045270.24328&radic&radic&radic&radic S000003951TPO1YLL028WverifiedPolyamine transporter that recognizes spermine, putrescine, andspermidine; catalyzes uptake of polyamines atalkaline pH and excretion at acidic pH;phosphorylation enhances activity and sortingto the plasma membrane 0.045040.24247&radic&radic&radic&radic&radic S000005460PKH2YOL100WverifiedSerine/threonine protein kinase involved insphingolipid-mediated signaling pathway thatcontrols endocytosis; activates Ypk1p andYkr2p, components of signaling cascade requiredfor maintenance of cell wall integrity;redundant with Pkh1p 0.0450.24236&radic S000002732UTP4YDR324CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.044970.24234&radic S000006319YPR115WUncharacterizedHypothetical protein 0.04430.23959&radic&radic S000005947SKS1YPL026CverifiedPutative serine/threonine protein kinase; involved in theadaptation to low concentrations of glucoseindependent of the SNF3 regulated pathway 0.044310.23959&radic&radic&radic S000002898PKH1YDR490CverifiedSerine/threonine protein kinase involved insphingolipid-mediated signaling pathway thatcontrols endocytosis; activates Ypk1p andYkr2p, components of signaling cascade requiredfor maintenance of cell wall integrity;redundant with Pkh2p 0.044280.23959 S000000934PMD1YER132CverifiedProtein with an N-terminal kelch-like domain, putative negativeregulator of early meiotic gene expression;required, with Mds3p, for growth under alkalineconditions 0.043770.23757&radic S000004478PPZ1YML016CverifiedSerine/threonine protein phosphatase Z, isoform of Ppz2p;involved in regulation of potassium transport,which affects osmotic stability, cell cycleprogression, and halotolerance 0.043560.23665&radic&radic&radic S000005501PPM2YOL141WverifiedPutative carboxyl methyl transferase, has similarity to Ppm1pbut biochemical activity not yet demonstrated;the authentic, non-tagged protein is detectedin highly purified mitochondria inhigh-throughput studies 0.043520.23665&radic S000003944SPA2YLL021WverifiedComponent of the polarisome, which functions in actincytoskeletal organization during polarizedgrowth; acts as a scaffold for Mkk1p and Mpk1pcell wall integrity signaling components;potential Cdc28p substrate &radic0.043310.23622&radic&radic S000000833YPT31YER031CverifiedGTPase of the Ypt/Rab family, very similar to Ypt32p; involvedin the exocytic pathway; mediates intra-Golgitraffic or the budding of post-Golgi vesiclesfrom the trans-Golgi 0.043210.2356&radic&radic S000004105CFT2YLR115WverifiedSubunit of the mRNA cleavage and polyadenlylation factor (CPF);required for pre-mRNA cleavage, polyadenylationand poly(A) site recognition, 43% similaritywith the mammalian CPSF-100 protein. 0.043210.2356&radic&radic&radic&radic&radic S000002554EKI1YDR147WverifiedEthanolamine kinase, primarily responsible forphosphatidylethanolamine synthesis via theCDP-ethanolamine pathway; also exhibits cholinekinase activity 0.043150.2355&radic S000001069RPN1YHR027CverifiedNon-ATPase base subunit of the 19S regulatory particle of the26S proteasome; may participate in therecognition of several ligands of theproteasome; contains a leucine-rich repeat(LRR) domain, a site for protein?proteininteractions 0.042870.23409&radic&radic S000004931PRE5YMR314Wverified20S proteasome alpha-type subunit 0.042910.23409&radic&radic S000003368LSB1YGR136WverifiedProtein containing an N-terminal SH3 domain; binds Las17p,which is a homolog of human Wiskott-AldrichSyndrome protein involved in actin patchassembly and actin polymerization 0.042680.23328&radic&radic&radic&radic S000004975HHF2YNL030WverifiedOne of two identical histone H4 proteins (see also HHF1); corehistone required for chromatin assembly andchromosome function; contributes to telomericsilencing; N-terminal domain involved inmaintaining genomic integrity 0.042420.23211&radic S000005886VTS1YOR359WverifiedPost-transcriptional gene regulator, RNA-binding proteincontaining a SAM domain; shows geneticinteractions with Vti1p, which is a v-SNAREinvolved in cis-Golgi membrane traffic 0.04230.23169&radic&radic&radic S000005620ARF3YOR094WverifiedGlucose-repressible ADP-ribosylation factor, GTPase of the Rassuperfamily involved in development of polarity 0.042250.23151&radic S000002753HXT3YDR345CverifiedLow affinity glucose transporter of the major facilitatorsuperfamily, expression is induced in low orhigh glucose conditions 0.04210.23108&radic&radic&radic&radic&radic S000000014TPD3YAL016WverifiedRegulatory subunit A of the heterotrimeric protein phosphatase2A, which also contains regulatory subunitCdc55p and either catalytic subunit Pph21p orPph22p; required for cell morphogenesis and fortranscription by RNA polymerase III &radic0.041280.22704&radic&radic S000004181PEX13YLR191WverifiedIntegral peroxisomal membrane receptor for the PTS1 peroxisomalmatrix protein signal recognition factor Pex5p,required for the translocation of peroxisomalmatrix proteins, also interacts with Pex7p andPex14p, contains a C-terminal SH3 domain 0.041280.22687&radic&radic&radic&radic S000000273TAT1YBR069CverifiedAmino acid transport protein for valine, leucine, isoleucine,and tyrosine, low-affinity tryptophan andhistidine transporter; overexpression confersFK506 resistance 0.041160.22644&radic S000000287TEC1YBR083WverifiedTranscription factor required for full Ty1 epxression,Ty1-mediated gene activation, and haploidinvasive and diploid pseudohyphal growth;TEA/ATTS DNA-binding domain family member 0.041160.22644&radic&radic S000005683PUP1YOR157CverifiedEndopeptidase with trypsin-like activity that cleaves afterbasic residues; beta-type subunit of 20Sproteasome synthesized as a proprotein beforebeing proteolytically processed for assemblyinto 20S particle; human homolog is subunit Z 0.041030.22596&radic&radic&radic&radic S000001198YSP1YHR155WuncharacterizedMitochondrial protein with a potential role in promotingmitochondrial fragmentation during programmedcell death in response to high levels ofalpha-factor mating pheromone or the drugamiodarone 0.040890.22533&radic S000004662FET3YMR058WverifiedFerro-O2-oxidoreductase required for high-affinity iron uptakeand involved in mediating resistance to copperion toxicity, belongs to class of integralmembrane multicopper oxidases 0.040780.22482&radic S000001126STE12YHR084WverifiedTranscription factor that is activated by a MAP kinasesignaling cascade, activates genes involved inmating or pseudohyphal/invasive growthpathways; cooperates with Tec1p transcriptionfactor to regulate genes specific for invasivegrowth 0.040570.22392&radic S000002905ITR1YDR497CverifiedMyo-inositol transporter with strong similarity to the minormyo-inositol transporter Itr2p, member of thesugar transporter superfamily; expression isrepressed by inositol and choline via Opi1p andderepressed via Ino2p and Ino4p 0.040560.22378&radic S000001859MOB2YFL034C-BverifiedComponent of the RAM signaling network, localizes and activatesthe Ace2p in the daughter cell nucleus todirect daughter cell-specific transcription ofseveral genes involved in cell separation;Mob1p-like protein &radic0.040530.22376&radic&radic&radic&radic S000000935GLC7YER133WverifiedCatalytic subunit of type 1 serine/threonine proteinphosphatase, involved in many processesincluding glycogen metabolism, sporulation, andmitosis; interacts with multiple regulatorysubunits; predominantly isolated with Sds22p &radic0.040050.22155&radic&radic S000003684RPA34YJL148WverifiedRNA polymerase I subunit A34.5 0.040010.22121&radic S000000333OPY1YBR129CverifiedProtein of unknown function, overproduction blocks cell cyclearrest in the presence of mating pheromone; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 0.039930.22079&radic S000005799TPO4YOR273CverifiedPolyamine transport protein, recognizes spermine, putrescine,and spermidine; localizes to the plasmamembrane; member of the major facilitatorsuperfamily 0.039910.22079&radic S000001861RIM15YFL033CverifiedGlucose-repressible protein kinase involved in signaltransduction during cell proliferation inresponse to nutrients, specifically theestablishment of stationary phase; originallyidentified as a regulator of IME2 0.039780.22037&radic S000001599PRR1YKL116CverifiedProtein kinase with a possible role in MAP kinase signaling inthe pheromone response pathway 0.039680.21986&radic&radic&radic&radic&radic S000000019CCR4YAL021CverifiedComponent of the CCR4-NOT transcriptional complex, which isinvolved in regulation of gene expression;component of the major cytoplasmic deadenylase,which is involved in mRNA poly(A) tailshortening 0.039610.21946&radic&radic&radic S000001808SKG1YKR100CuncharacterizedTransmembrane protein of unknown function; localizes on theinner surface of the plasma membrane at the budand in the daughter cell; affects the cell wallpolymer composition 0.039020.2163&radic S000002971CDH1YGL003CverifiedCell-cycle regulated activator of the anaphase-promotingcomplex/cyclosome (APC/C), which directsubiquitination of mitotic cyclins resulting inexit from mitosis; targets the APC/C tospecific substrates including CDC20, ASE1 andCIN8 0.038850.21597&radic&radic&radic S000005227WSC2YNL283CverifiedPartially redundant sensor-transducer of the stress-activatedPKC1-MPK1 signaling pathway involved inmaintenance of cell wall integrity and recoveryfrom heat shock; secretory pathway Wsc2p isrequired for the arrest of secretion response 0.038730.21504&radic&radic&radic&radic S000002285PCL2YDL127WverifiedG1 cyclin, associates with Pho85p cyclin-dependent kinase (Cdk)to contribute to entry into the mitotic cellcycle, essential for cell morphogenesis;localizes to sites of polarized cell growth 0.038520.21365 S000006310ISR1YPR106WverifiedPredicted protein kinase, overexpression causes sensitivity tostaurosporine, which is a potent inhibitor ofprotein kinase C 0.038520.21365&radic&radic&radic S000006295YPR091CUncharacterizedHypothetical protein 0.03850.21365 S000005615VPS21YOR089CverifiedGTPase required for transport during endocytosis and forcorrect sorting of vacuolar hydrolases;localized in endocytic intermediates; detectedin mitochondria; geranylgeranylation requiredfor membrane association; mammalian Rab5homolog 0.038280.21281&radic&radic&radic&radic S000003290YGR058WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 0.03790.21078&radic&radic&radic&radic S000005398PRE6YOL038Wverified20S proteasome alpha-type subunit; green fluorescent protein(GFP)-fusion protein relocates from the cytosolto the mitochondrial outer surface uponoxidative stress 0.037790.21005&radic&radic&radic&radic&radic S000000763RAD23YEL037CverifiedProtein with ubiquitin-like N terminus, recognizes and bindsdamaged DNA (with Rad4p) during nucleotideexcision repair; regulates Rad4p levels,subunit of Nuclear Excision Repair Factor 2(NEF2); homolog of human HR23A and HR23Bproteins 0.037510.20821&radic&radic&radic&radic&radic S000006328CTR1YPR124WverifiedHigh-affinity copper transporter of the plasma membrane,mediates nearly all copper uptake under lowcopper conditions; transcriptionally induced atlow copper levels and degraded at high copperlevels 0.037240.20723&radic&radic&radic S000005117MDG1YNL173CverifiedPlasma membrane protein involved in G-protein mediatedpheromone signaling pathway; overproductionsuppresses bem1 mutations 0.037090.20619&radic&radic S000003297VHT1YGR065CverifiedHigh-affinity plasma membrane H+-biotin (vitamin H) symporter;mutation results in fatty acid auxotrophy; 12transmembrane domain containing majorfacilitator subfamily member; mRNA levelsnegatively regulated by iron deprivation andbiotin 0.037070.20619&radic&radic S000005569WHI2YOR043WverifiedProtein required, with binding partner Psr1p, for fullactivation of the general stress response,possibly through Msn2p dephosphorylation;regulates growth during the diauxic shift;negative regulator of G1 cyclin expression 0.037020.20619&radic S000005878MEK1YOR351CverifiedMeiosis-specific serine/threonine protein kinase, functions inmeiotic checkpoint, phosphorylates Red1p,interacts with Hop1p; required for meioticrecombination and normal spore viability 0.036930.20572&radic&radic S000006184KEL3YPL263CverifiedCytoplasmic protein of unknown function 0.03690.20555&radic&radic&radic S000005335POP2YNR052CverifiedRNase of the DEDD superfamily, subunit of the Ccr4-Not complexthat mediates 3' to 5' mRNA deadenylation 0.036860.20532&radic S000005098YCK2YNL154CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck1p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck1p &radic0.036680.20487&radic&radic&radic&radic S000005037YPT53YNL093WverifiedGTPase, similar to Ypt51p and Ypt52p and to mammalian rab5;required for vacuolar protein sorting andendocytosis 0.036610.20446 S000000258YRO2YBR054WverifiedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies;transcriptionally regulated by Haa1p 0.036630.20446&radic S000000046GEM1YAL048CverifiedEvolutionarily-conserved tail-anchored outer mitochondrialmembrane GTPase which regulates mitochondrialmorphology; cells lacking Gem1p containcollapsed, globular, or grape-likemitochondria; not required forpheromone-induced cell death 0.036490.20369 S000002511SPO71YDR104CverifiedMeiosis-specific protein of unknown function, required forspore wall formation during sporulation;dispensable for both nuclear divisions duringmeiosis 0.036460.2036 S000002979SCL1YGL011CverifiedAlpha subunit of the 20S core complex of the 26S proteasomeinvolved in the degradation of ubiquitinatedsubstrates; essential for growth; detected inthe mitochondria 0.036140.20105&radic&radic&radic&radic S000000914LSM4YER112WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.036050.20068&radic&radic S000003694CIS3YJL158CverifiedMannose-containing glycoprotein constituent of the cell wall;member of the PIR (proteins with internalrepeats) family 0.036010.20054&radic S000002860PPN1YDR452WverifiedVacuolar endopolyphosphatase with a role in phosphatemetabolism; functions as a homodimer 0.035730.19919 S000005711GSP2YOR185CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; interacts withKap121p, Kap123p and Pdr6p (karyophilin betas);Gsp1p homolog that is not required forviability 0.035770.19919&radic&radic&radic&radic S000002520PDS1YDR113CverifiedSecurin that inhibits anaphase by binding separin Esp1p, alsoblocks cyclin destruction and mitotic exit,essential for cell cycle arrest in mitosis inthe presence of DNA damage or aberrant mitoticspindles; also present in meiotic nuclei 0.035560.19794&radic&radic&radic&radic S000000687KIN82YCR091WverifiedPutative serine/threonine protein kinase, most similar tocyclic nucleotide-dependent protein kinasesubfamily and the protein kinase C subfamily 0.035650.19794&radic&radic S000000925YCK3YER123WverifiedPalmitoylated, vacuolar membrane-localized casein kinase Iisoform; negatively regulates vacuole fusionduring hypertonic stress via phosphorylation ofthe HOPS complex subunit, Vps41p; sharesoverlapping essential functions with Hrr25p 0.035580.19794 S000005977YPL056CUncharacterizedHypothetical protein 0.03550.19782&radic S000001409SLN1YIL147CverifiedHistidine kinase osmosensor that regulates a MAP kinasecascade; transmembrane protein with anintracellular kinase domain that signals toYpd1p and Ssk1p, thereby forming a phosphorelaysystem similar to bacterial two-componentregulators 0.035420.19689&radic S000002510STE5YDR103WverifiedPheromone-responsive scaffold protein; shuttles from thenucleus to the plasma membrane and assembleskinases Ste11p, Ste7p, and Fus3p into aspecific signaling complex; active oligomericform interacts with Ste4p-Ste18p complex 0.035150.19567&radic&radic&radic&radic&radic&radic S000001587GFA1YKL104CverifiedGlutamine-fructose-6-phosphate amidotransferase, catalyzes theformation of glucosamine-6-P and glutamate fromfructose-6-P and glutamine in the first step ofchitin biosynthesis 0.035080.19533&radic S000005889PRE10YOR362Cverified20S proteasome alpha-type subunit 0.035090.19533&radic&radic S000003462BNS1YGR230WverifiedProtein with some similarity to Spo12p; overexpression bypassesneed for Spo12p, but not required for meiosis 0.035030.195&radic&radic S000004034PDC1YLR044CverifiedMajor of three pyruvate decarboxylase isozymes, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde; subject to glucose-, ethanol-,and autoregulation; involved in amino acidcatabolism 0.035030.195&radic&radic&radic&radic&radic&radic S000002232BRE1YDL074CverifiedE3 ubiquitin ligase for Rad6p, required for the ubiquitinationof histone H2B, recruitment of Rad6p topromoter chromatin and subsequent methylationof histone H3 (on L4 and L79), contains RINGfinger domain 0.034980.19494&radic S000001277BAR1YIL015WverifiedAspartyl protease secreted into the periplasmic space of matingtype a cells, helps cells find mating partners,cleaves and inactivates alpha factor allowingcells to recover from alpha-factor-induced cellcycle arrest 0.034770.19383&radic S000004537TCB3YML072CverifiedLipid-binding protein, localized to the bud via specific mRNAtransport; non-tagged protein localizes tomitochondria and GFP-fusion protein localizesto the cell periphery; C-termini of Tcb1p,Tcb2p and Tcb3p interact 0.034760.19383&radic S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.034510.19225&radic S000001194YHR151CUncharacterizedPutative protein of unknown function 0.034430.19209 S000002909PLM2YDR501WverifiedProtein required for partitioning of the 2-micron plasmid 0.034120.19018 S000000138FUI1YBL042CverifiedHigh affinity uridine permease, localized to the plasmamembrane; not involved in uracil transport 0.034050.18978&radic S000005127NPR1YNL183CverifiedProtein kinase that stabilizes several plasma membrane aminoacid transporters by antagonizing theirubiquitin-mediated degradation 0.034070.18978&radic&radic&radic S000003942KNS1YLL019CverifiedNonessential putative protein kinase of unknown cellular role;member of the LAMMER family of protein kinases,which are serine/threonine kinases also capableof phosphorylating tyrosine residues 0.033970.18963&radic&radic&radic S000003865SOD1YJR104CverifiedCytosolic superoxide dismutase; some mutations are analogous tothose that cause ALS (amyotrophic lateralsclerosis) in humans 0.03390.18923&radic&radic&radic S000003284FMP48YGR052WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.033570.18748&radic&radic&radic&radic S000002344TFP1YDL185WverifiedVacuolar ATPase V1 domain subunit A containing the catalyticnucleotide binding sites; protein precursorundergoes self-catalyzed splicing to yield theextein Tfp1p and the intein Vde (PI-SceI),which is a site-specific endonuclease 0.033460.1867 S000003148ATG1YGL180WverifiedProtein serine/threonine kinase, required for autophagy and forthe cytoplasm-to-vacuole targeting (Cvt)pathway 0.033450.1867 S000000242CHS2YBR038WverifiedChitin synthase II, requires activation from zymogenic form inorder to catalyze the transfer ofN-acetylglucosamine (GlcNAc) to chitin;required for the synthesis of chitin in theprimary septum during cytokinesis &radic0.033260.18594 S000001748YKR040CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W 0.032920.18383 S000000979BMH1YER177Wverified14-3-3 protein, major isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.032860.1836&radic S000006161HSP82YPL240CverifiedCytoplasmic chaperone (Hsp90 family) required for pheromonesignaling and negative regulation of Hsf1p;docks with the mitochondrial import receptorTom70p for preprotein delivery; interacts withco-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p 0.032670.18249&radic&radic&radic&radic S000002859YHP1YDR451CverifiedOne of two homeobox transcriptional repressors (see alsoYox1p), that bind to Mcm1p and to early cellcycle box (ECB) elements of cell cycleregulated genes, thereby restrictingECB-mediated transcription to the M/G1 interval 0.032680.18249&radic S000004905YMR291WUncharacterizedPutative kinase of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR291W is not an essential gene 0.032560.18183&radic&radic&radic&radic S000003394TIF4631YGR162WverifiedTranslation initiation factor eIF4G, subunit of the mRNAcap-binding protein complex (eIF4F) that alsocontains eIF4E (Cdc33p); associates with thepoly(A)-binding protein Pab1p, also interactswith eIF4A (Tif1p); homologous to Tif4632p 0.032490.18141&radic S000000274ALG14YBR070CverifiedComponent of UDP-GlcNAc transferase required for the secondstep of dolichyl-linked oligosaccharidesynthesis; anchors the catalytic subunit Alg13pto the ER membrane; similar to bacterial andhuman glycosyltransferases 0.032480.18141&radic S000001946PRE4YFR050Cverified20S proteasome beta-type subunit 0.032420.18085&radic&radic&radic&radic S000000282ECM33YBR078WverifiedGPI-anchored protein of unknown function, has a possible rolein apical bud growth; GPI-anchoring on theplasma membrane crucial to function; similar toSps2p and Pst1p 0.03230.18013 S000006189PLC1YPL268WverifiedPhosphoinositide-specific phospholipase C, hydrolyzesphosphatidylinositol 4,5-biphosphate (PIP2) togenerate inositol 1,4,5-triphosphate (IP3) and1,2-diacylglycerol (DAG); involved inkinetochore function and pseudohyphaldifferentiation 0.032260.17989 S000006152FAS2YPL231WverifiedAlpha subunit of fatty acid synthetase, which catalyzes thesynthesis of long-chain saturated fatty acids;contains beta-ketoacyl reductase andbeta-ketoacyl synthase activities 0.032240.17966&radic&radic&radic S000001566YKL083WDubiousDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 0.031950.17778&radic&radic S000002314CLB3YDL155WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the G2/M transition; may beinvolved in DNA replication and spindleassembly; accumulates during S phase and G2,then targeted for ubiquitin-mediateddegradation 0.031970.17778&radic&radic S000005379YOL019WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole 0.031850.17697&radic&radic&radic&radic&radic S000004723SPC24YMR117CverifiedComponent of the evolutionarily conservedkinetochore-associated Ndc80 complex(Ndc80p-Nuf2p-Spc24p-Spc25p); involved inchromosome segregation, spindle checkpointactivity and kinetochore clustering 0.031860.17697 S000005266KRE1YNL322CverifiedCell wall glycoprotein involved in beta-glucan assembly; servesas a K1 killer toxin membrane receptor 0.031810.17697&radic&radic S000000602RVS161YCR009CverifiedAmphiphysin-like lipid raft protein; subunit of a complex(Rvs161p-Rvs167p) that regulates polarizationof the actin cytoskeleton, endocytosis, cellpolarity, cell fusion and viability followingstarvation or osmotic stress 0.031820.17697&radic&radic&radic&radic S000005082SRV2YNL138WverifiedCAP (cyclase-associated protein) subunit of adenylyl cyclasecomplex; N-terminus binds adenylyl cyclase andfacilitates activation by RAS; C-terminus bindsADP-actin monomers, facilitating regulation ofactin dynamics and cell morphogenesis 0.031840.17697 S000000822GPA2YER020WverifiedNucleotide binding alpha subunit of the heterotrimeric Gprotein that interacts with the receptor Gpr1p,has signaling role in response to nutrients;green fluorescent protein (GFP)-fusion proteinlocalizes to the cell periphery 0.031490.17485&radic&radic&radic&radic S000004722ASC1YMR116CverifiedWD repeat protein (G-beta like protein) involved in translationregulation; required for repression of Gcn4pactivity in the absence of amino-acidstarvation; core component of the ribosome;ortholog of mammalian RACK1 0.031410.17452 S000001654YKL171WUncharacterizedPutative protein of unknown function; predicted protein kinase; implicated in proteasome function; epitope-tagged protein localizes to the cytoplasm 0.031350.17432&radic&radic&radic&radic S000001750UTH1YKR042WverifiedMitochondrial outer membrane and cell wall localized SUN familymember required for mitochondrial autophagy;involved in the oxidative stress response, lifespan during starvation, mitochondrialbiogenesis, and cell death 0.031330.17423&radic S000005584ASE1YOR058CverifiedMember of a family of microtubule-associated proteins (MAPs)that function at the mitotic spindle midzone;required for spindle elongation; undergoes cellcycle-regulated degradation by anaphasepromoting complex; potential Cdc28p substrate 0.031330.17423&radic&radic S000005116APC1YNL172WverifiedLargest subunit of the Anaphase-Promoting Complex/Cyclosome(APC/C), which is a ubiquitin-protein ligaserequired for degradation of anaphaseinhibitors, including mitotic cyclins, duringthe metaphase/anaphase transition 0.031270.17378&radic&radic&radic&radic&radic S000005372HTZ1YOL012CverifiedHistone variant H2AZ, exchanged for histone H2A in nucleosomesby the SWR1 complex; involved intranscriptional regulation through preventionof the spread of silent heterochromatin 0.031150.17267&radic&radic&radic S000001186DSE2YHR143WverifiedDaughter cell-specific secreted protein with similarity toglucanases, degrades cell wall from thedaughter side causing daughter to separate frommother; expression is repressed by cAMP 0.031060.17244&radic&radic&radic S000005134YNL190WUncharacterizedHypothetical protein 0.030950.17185&radic&radic&radic&radic&radic S000002674YDR266CUncharacterizedHypothetical protein 0.030930.17178&radic&radic&radic&radic S000002269RRP42YDL111CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp4p, Rrp41p,Rrp43p and Dis3p 0.030830.17115&radic&radic S000002435REG1YDR028CverifiedRegulatory subunit of type 1 protein phosphatase Glc7p,involved in negative regulation ofglucose-repressible genes 0.030760.17076&radic S000000622RHB1YCR027CverifiedPutative Rheb-related GTPase involved in regulating canavanineresistance and arginine uptake; member of theRas superfamily of G-proteins 0.030770.17076&radic S000002294RDI1YDL135CverifiedRho GDP dissociation inhibitor involved in the localization andregulation of Cdc42p 0.030720.17044&radic&radic S000005330YNR047WUncharacterizedPutative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; potential Cdc28p substrate 0.030650.16936&radic S000004291EXG1YLR300WverifiedMajor exo-1,3-beta-glucanase of the cell wall, involved in cellwall beta-glucan assembly; exists as threedifferentially glycosylated isoenzymes 0.030650.16936&radic S000000813TIR1YER011WverifiedCell wall mannoprotein of the Srp1p/Tip1p family ofserine-alanine-rich proteins; expression isdownregulated at acidic pH and induced by coldshock and anaerobiosis; abundance is increasedin cells cultured without shaking 0.030670.16936&radic&radic&radic S000000342YBR138CUncharacterizedCytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene 0.030530.16907&radic S000005429HST1YOL068CverifiedNAD(+)-dependent histone deacetylase; essential subunit of theSum1p/Rfm1p/Hst1p complex required forORC-dependent silencing and mitotic repression;non-essential subunit of the Set3C deacetylasecomplex; involved in telomere maintenance 0.030470.16857&radic&radic&radic&radic&radic S000003241SEC9YGR009Cverifiedt-SNARE protein important for fusion of secretory vesicles withthe plasma membrane; similar to but notfunctionally redundant with Spo20p; SNAP-25homolog 0.030360.16764&radic&radic&radic S000000025YAL027WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YAL027W is a non-essential gene 0.030120.16612&radic&radic S000004238RCK2YLR248WverifiedProtein kinase involved in the response to oxidative andosmotic stress; identified as suppressor of S.pombe cell cycle checkpoint mutations 0.030080.16592&radic&radic&radic&radic S000002506BMH2YDR099Wverified14-3-3 protein, minor isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.029910.16441&radic&radic&radic S000004046ERG3YLR056WverifiedC-5 sterol desaturase, catalyzes the introduction of a C-5(6)double bond into episterol, a precursor inergosterol biosynthesis; mutants are viable,but cannot grow on non-fermentable carbonsources 0.029570.16199&radic S000000669SSK22YCR073CverifiedMAP kinase kinase kinase of the HOG1 mitogen-activatedsignaling pathway; functionally redundant with,and homologous to, Ssk2p; interacts with and isactivated by Ssk1p; phosphorylates Pbs2p 0.029610.16199 S000000137PRE7YBL041Wverified20S proteasome beta-type subunit 0.029480.161 S000006045SPC29YPL124WverifiedInner plaque spindle pole body (SPB) component, links thecentral plaque component Spc42p to the innerplaque component Spc110p; required for SPBduplication 0.029380.16024&radic&radic S000004128NHA1YLR138WverifiedNa+/H+ antiporter involved in sodium and potassium effluxthrough the plasma membrane; required foralkali cation tolerance at acidic pH 0.029360.16013&radic&radic&radic&radic S000002908RPL37BYDR500CverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to Rpl37Ap and to rat L37 ribosomalprotein 0.029340.16002&radic S000006256NHP6AYPR052CverifiedHigh-mobility group non-histone chromatin protein, functionallyredundant with Nhp6Bp; homologous to mammalianhigh mobility group proteins 1 and 2; acts torecruit transcription factor Rcs1p to certainpromoters 0.029340.15996&radic S000000098HTB2YBL002WverifiedOne of two nearly identical (see HTB1) histone H2B subtypesrequired for chromatin assembly and chromosomefunction; Rad6p-Bre1p-Lge1p mediatedubiquitination regulates transcriptionalactivation, meiotic DSB formation and H3methylation 0.029340.15996&radic&radic&radic&radic S000001644YKL161CVerifiedProtein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; associates with Rlm1p 0.029250.15921&radic&radic&radic S000000026FRT2YAL028WverifiedTail-anchored endoplasmic reticulum membrane protein, interactswith homolog Frt1p but is not a substrate ofcalcineurin (unlike Frt1p), promotes growth inconditions of high Na+, alkaline pH, or cellwall stress; potential Cdc28p substrate 0.029210.1587&radic&radic&radic&radic S000001121IRE1YHR079CverifiedSerine-threonine kinase and endoribonuclease; transmembraneprotein that initiates the unfolded proteinresponse signal by regulating synthesis ofHac1p through HAC1 mRNA splicing 0.028920.1567&radic S000004521CMP2YML057WverifiedCalcineurin A; one isoform (the other is CNA1) of the catalyticsubunit of calcineurin, aCa++/calmodulin-regulated protein phosphatasewhich regulates Crz1p (a stress-responsetranscription factor), the other calcineurinsubunit is CNB1 0.028720.15512&radic S000005899NDD1YOR372CverifiedTranscriptional activator essential for nuclear division;localized to the nucleus; essential componentof the mechanism that activates the expressionof a set of late-S-phase-specific genes 0.028730.15512&radic&radic&radic S000003245SNU71YGR013WverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; yeast specific, no metazoancounterpart 0.028670.15483&radic&radic&radic&radic S000000015PSK1YAL017WverifiedOne of two (see also PSK2) PAS domain containing S/T proteinkinases; coordinately regulates proteinsynthesis and carbohydrate metabolism andstorage in response to a unknown metabolitethat reflects nutritional status 0.028620.15441&radic&radic&radic&radic S000002637IVY1YDR229WverifiedPhospholipid-binding protein that interacts with both Ypt7p andVps33p, may partially counteract the action ofVps33p and vice versa, localizes to the rim ofthe vacuole as cells approach stationary phase 0.02860.15414&radic&radic&radic S000001420YIL158WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 0.028530.15362 S000004922YMR306C-ADubiousHypothetical protein 0.02850.15362 S000000343YBR139WUncharacterizedPutative serine type Carboxypeptidase; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YBR139W is not an essential gene 0.028420.15311 S000003174CHC1YGL206CverifiedClathrin heavy chain, subunit of the major coat proteininvolved in intracellular protein transport andendocytosis; two heavy chains form the clathrintriskelion structural component; the lightchain (CLC1) is thought to regulate function 0.028430.15311 S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.028290.15198&radic&radic S000005405PSK2YOL045WverifiedOne of two (see also PSK1) PAS domain containing S/T proteinkinases; regulates sugar flux and translationin response to an unknown metabolite byphosphorylating Ugp1p and Gsy2p (sugar flux)and Caf20p, Tif11p and Sro9p (translation) 0.028270.15198&radic&radic&radic&radic S000004517YML053CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YML053C is not an essential gene 0.028250.15184&radic&radic S000004444SST2YLR452CverifiedGTPase-activating protein for Gpa1p, regulates desensitizationto alpha factor pheromone; also required toprevent receptor-independent signaling of themating pathway; member of the RGS (regulator ofG-protein signaling) family 0.028190.15144&radic S000001801PTR2YKR093WverifiedIntegral membrane peptide transporter, mediates transport ofdi- and tri-peptides; conserved protein thatcontains 12 transmembrane domains; PTR2expression is regulated by the N-end rulepathway via repression by Cup9p 0.028170.15129&radic S000001681PTK1YKL198CverifiedPutative serine/threonine protein kinase that regulatesspermine uptake; involved in polyaminetransport; possible mitochondrial protein 0.028090.15065&radic&radic S000004264CDC46YLR274WverifiedComponent of the hexameric MCM complex, which is important forpriming origins of DNA replication in G1 andbecomes an active ATP-dependent helicase thatpromotes DNA melting and elongation whenactivated by Cdc7p-Dbf4p in S-phase 0.028120.15065&radic&radic S000002764SPC110YDR356WverifiedInner plaque spindle pole body (SPB) component, ortholog ofhuman kendrin; involved in connecting nuclearmicrotubules to SPB; interacts withTub4p-complex and calmodulin; phosphorylated byMps1p in cell cycle-dependent manner 0.028040.1505&radic&radic&radic S000000922SCS2YER120WverifiedIntegral ER membrane protein that regulates phospholipidmetabolism via an interaction with the FFATmotif of Opi1p, also involved in telomericsilencing, disruption causes inositolauxotrophy above 34 degrees C, VAP homolog 0.0280.14985 S000003732ELO1YJL196CverifiedElongase I, medium-chain acyl elongase, catalyzescarboxy-terminal elongation of unsaturatedC12-C16 fatty acyl-CoAs to C16-C18 fatty acids 0.027970.14985 S000006258SMK1YPR054WverifiedMiddle sporulation-specific mitogen-activated protein kinase(MAPK) required for production of the outerspore wall layers; negatively regulatesactivity of the glucan synthase subunit Gsc2p 0.027950.14985 S000003126RCK1YGL158WverifiedProtein kinase involved in the response to oxidative stress;identified as suppressor of S. pombe cell cyclecheckpoint mutations 0.0280.14985 S000005561SHE4YOR035CverifiedProtein containing a UCS (UNC-45/CRO1/SHE4) domain, binds tomyosin motor domains to regulate myosinfunction; involved in endocytosis, polarizationof the actin cytoskeleton, and asymmetric mRNAlocalization 0.027880.14941&radic&radic&radic&radic S000001651KKQ8YKL168CverifiedPutative serine/threonine protein kinase with unknown cellularrole 0.027830.14916&radic&radic&radic S000003642IME2YJL106WverifiedSerine/threonine protein kinase involved in activation ofmeiosis, associates with Ime1p and mediates itsstability, activates Ndt80p; IME2 expression ispositively regulated by Ime1p 0.027810.14903 S000002931SPS1YDR523CverifiedPutative protein serine/threonine kinase expressed at the endof meiosis and localized to the prosporemembrane, required for correct localization ofenzymes involved in spore wall synthesis 0.027630.148&radic&radic S000000036CDC19YAL038WverifiedPyruvate kinase, functions as a homotetramer in glycolysis toconvert phosphoenolpyruvate to pyruvate, theinput for aerobic (TCA cycle) or anaerobic(glucose fermentation) respiration 0.027590.14744&radic&radic&radic S000003871YMR1YJR110WverifiedPhosphatidylinositol 3-phosphate [PI(3)P] phosphatase,regulates the localization and levels ofPI(3)P; involved in cytoplasm to vacuole (CVT)transport; has similarity to the conservedmyotubularin dual specificity phosphatasefamily 0.027510.14726&radic S000005089MFA2YNL145WverifiedMating pheromone a-factor, made by a cells; interacts withalpha cells to induce cell cycle arrest andother responses leading to mating; biogenesisinvolves C-terminal modification, N-terminalproteolysis, and export; also encoded by MFA1 0.027490.14709 S000005612TCB1YOR086CverifiedLipid-binding protein containing three calcium and lipidbinding domains; non-tagged protein localizesto mitochondria and GFP-fusion proteinlocalizes to the cell periphery; C-termini ofTcb1p, Tcb2p and Tcb3p interact 0.027450.14697&radic&radic S000005793HRK1YOR267CverifiedProtein kinase implicated in activation of the plasma membraneH(+)-ATPase Pma1p in response to glucosemetabolism; plays a role in ion homeostasis 0.027440.1468&radic S000002103PMP2YEL017C-AverifiedProteolipid associated with plasma membrane H(+)-ATPase(Pma1p); regulates plasma membrane H(+)-ATPaseactivity; nearly identical to PMP1 0.027210.14553&radic&radic&radic S000005772YOR246CUncharacterizedProtein with similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts 0.027160.14523 S000000789CAN1YEL063CverifiedPlasma membrane arginine permease, requires phosphatidylethanolamine (PE) for localization, exclusivelyassociated with lipid rafts; mutation conferscanavanine resistance 0.027070.14471&radic&radic&radic&radic S000005380TAT2YOL020WverifiedHigh affinity tryptophan and tyrosine permease, overexpressionconfers FK506 resistance 0.026990.14394&radic&radic&radic&radic S000003737ECM25YJL201WverifiedNon-essential protein of unknown function; promoter contains aconsensus binding sequence for factor Abf1p 0.02660.14202&radic S000001924CDC14YFR028CverifiedProtein phosphatase required for mitotic exit; located in thenucleolus until liberated by the FEAR andMitotic Exit Network in anaphase, enabling itto act on key substrates to effect a decreasein CDK/B-cyclin activity and mitotic exit 0.02650.1414 S000006353NCE102YPR149WverifiedProtein of unknown function; contains transmembrane domains;involved in secretion of proteins that lackclassical secretory signal sequences; componentof the detergent-insoluble glycolipid-enrichedcomplexes (DIGs) 0.026410.14095&radic&radic S000002603REF2YDR195WverifiedRNA-binding protein involved in the cleavage step of mRNA3'-end formation prior to polyadenylation, andin snoRNA maturation; part of holo-CPFsubcomplex APT, which associates with 3'-endsof snoRNA- and mRNA-encoding genes 0.026420.14095&radic&radic&radic&radic&radic S000000897RAD51YER095WverifiedStrand exchange protein, forms a helical filament with DNA thatsearches for homology; involved in therecombinational repair of double-strand breaksin DNA during vegetative growth and meiosis;homolog of Dmc1p and bacterial RecA protein 0.026370.14078&radic&radic S000004921SCW10YMR305CverifiedCell wall protein with similarity to glucanases; may play arole in conjugation during mating based onmutant phenotype and its regulation by Ste12p 0.026360.14045&radic&radic S000004516SUR7YML052WverifiedPutative integral membrane protein; component of eisosomes;associated with endocytosis, along with Pil1pand Lsp1p; sporulation and plasma membranesphingolipid content are altered in mutants 0.026360.14045 S000005666SFL1YOR140WverifiedTranscription repressor involved in regulation offlocculation-related genes, inhibitstranscription by recruiting general corepressorCyc8p-Tup1p to different promoters; negativelyregulated by cAMP-dependent protein kinase Asubunit Tpk2p 0.026210.1396&radic&radic&radic&radic S000000896PUP3YER094CverifiedBeta subunit of the 20S proteasome involved inubiquitin-dependent catabolism; human homologis subunit C10 0.026150.13951 S000004829SKY1YMR216CverifiedSR protein kinase (SRPK) involved in regulating proteinsinvolved in mRNA metabolism and cationhomeostasis; similar to human SRPK1 0.026130.13935&radic&radic&radic&radic S000003258YGR026WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 0.026090.13912&radic&radic&radic&radic S000001622CTK1YKL139WverifiedCatalytic (alpha) subunit of C-terminal domain kinase I(CTDK-I), which phosphorylates the C-terminalrepeated domain of the RNA polymerase II largesubunit (Rpo21p) to affect both transcriptionand pre-mRNA 3' end processing 0.02610.13912&radic&radic&radic&radic&radic S000004557PRE8YML092Cverified20S proteasome beta-type subunit 0.026070.13912&radic S000001144KIC1YHR102WverifiedProtein kinase of the PAK/Ste20 kinase family, required forcell integrity possibly through regulating1,6-beta-glucan levels in the wall; physicallyinteracts with Cdc31p (centrin), which is acomponent of the spindle pole body 0.026050.1389&radic S000001555STB6YKL072WverifiedProtein that binds Sin3p in a two-hybrid assay 0.026050.1389&radic&radic&radic S000004811CIK1YMR198WverifiedKinesin-associated protein required for both karyogamy andmitotic spindle organization, interacts stablyand specifically with Kar3p and may function totarget this kinesin to a specific cellularrole; has similarity to Vik1p 0.025980.1386 S000003449CCH1YGR217WverifiedVoltage-gated high-affinity calcium channel involved in calciuminflux in response to some environmentalstresses as well as exposure to matingpheromones; interacts and co-localizes withMid1p, suggesting Cch1p and Mid1p functiontogether 0.025950.13844&radic&radic S000002266KIN28YDL108WverifiedSerine/threonine protein kinase, subunit of the transcriptionfactor TFIIH; involved in transcriptioninitiation at RNA polymerase II promoters 0.025950.13844&radic S000005565CKB2YOR039WverifiedBeta' regulatory subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA1, CKA2 and CKB1, the many substratesinclude transcription factors and all RNApolymerases 0.025880.13812&radic&radic&radic&radic S000003612NET1YJL076WverifiedCore subunit of the RENT complex, which is a complex involvedin nucleolar silencing and telophase exit;stimulates transcription by RNA polymerase Iand regulates nucleolar structure 0.025880.13812 S000000340MEC1YBR136WverifiedGenome integrity checkpoint protein and PI kinase superfamilymember; signal transducer required for cellcycle arrest and transcriptional responsesprompted by damaged or unreplicated DNA;monitors and participates in meioticrecombination 0.025890.13812 S000005827RAX1YOR301WverifiedProtein involved in bud site selection during bipolar budding;localization requires Rax2p; has similarity tomembers of the insulin-related peptidesuperfamily &radic0.025750.1374 S000000803MNN1YER001WverifiedAlpha-1,3-mannosyltransferase, integral membrane glycoproteinof the Golgi complex, required for addition ofalpha1,3-mannose linkages to N-linked andO-linked oligosaccharides, one of five S.cerevisiae proteins of the MNN1 family 0.025740.1374&radic&radic&radic&radic S000001721PRY2YKR013WverifiedProtein of unknown function, has similarity to Pry1p and Pry3pand to the plant PR-1 class of pathogen relatedproteins 0.02560.13649&radic S000002237MRK1YDL079CverifiedGlycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3homologs in S. cerevisiae that function toactivate Msn2p-dependent transcription ofstress responsive genes and that function inprotein degradation 0.025590.13649 S000001368MOB1YIL106WverifiedComponent of the mitotic exit network; associates with and isrequired for the activation andCdc15p-dependent phosphorylation of the Dbf2pkinase; required for cytokinesis and cellseparation; component of the CCR4transcriptional complex &radic0.02560.13649&radic S000003494BUD32YGR262CverifiedProtein kinase proposed to be involved in bud-site selection,telomere uncapping and elongation, andtranscription; component of the EKC/KEOPSprotein complex with Kae1p, Cgi121p, Pcc1p, andGon7p 0.025570.13647&radic&radic&radic S000005338HOL1YNR055CverifiedPutative ion transporter similar to the major facilitatorsuperfamily of transporters; mutations inmembrane-spanning domains permit nonselectivecation uptake 0.025550.13629&radic S000002379CDC13YDL220CverifiedSingle stranded DNA-binding protein found at TG1-3 telomereG-tails; regulates telomere replication throughrecruitment of specific sub-complexes, but theessential function is telomere capping 0.025480.13593&radic&radic S000006324CLB5YPR120CverifiedB-type cyclin involved in DNA replication during S phase;activates Cdc28p to promote initiation of DNAsynthesis; functions in formation of mitoticspindles along with Clb3p and Clb4p; mostabundant during late G1 phase 0.025420.1355&radic&radic&radic S000002161MCD1YDL003WverifiedEssential protein required for sister chromatid cohesion inmitosis and meiosis; subunit of the cohesincomplex; expression is cell cycle regulated andpeaks in S phase 0.025330.13511&radic&radic S000005436MDM20YOL076WverifiedNon-catalytic subunit of the NatB N-terminal acetyltransferase,which catalyzes acetylation of theamino-terminal methionine residues of allproteins beginning with Met-Asp or Met-Glu andof some proteins beginning with Met-Asn orMet-Met; involved in mitochondrial inheritanceand actin assembly 0.025280.1345&radic&radic S000005969CAM1YPL048WverifiedHomolog of translational cofactor elongation factor-1 gamma,participates in the regulation of GTP-bindingprotein EF-1 alpha, may play a redundant rolein the regulation of protein synthesis oranother GTP-dependent process 0.025080.13377&radic&radic&radic S000003225ADH4YGL256WverifiedAlcohol dehydrogenase type IV, dimeric enzyme demonstrated tobe zinc-dependent despite sequence similarityto iron-activated alcohol dehydrogenases;transcription is induced in response to zincdeficiency; alcohol dehydrogenase isoenzyme IV 0.025180.13377 S000004849TAF9YMR236WverifiedSubunit (17 kDa) of TFIID and SAGA complexes, involved in RNApolymerase II transcription initiation and inchromatin modification, similar to histone H3 0.025050.13358&radic&radic S000000615HSP30YCR021CverifiedHydrophobic plasma membrane localized, stress-responsiveprotein that negatively regulates theH(+)-ATPase Pma1p; induced by heat shock,ethanol treatment, weak organic acid, glucoselimitation, and entry into stationary phase 0.025050.13358&radic&radic&radic&radic S000000070ADE1YAR015WverifiedN-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR)synthetase, required for 'de novo' purinenucleotide biosynthesis; red pigmentaccumulates in mutant cells deprived of adenine 0.0250.13318&radic&radic&radic S000005169CNM67YNL225CverifiedComponent of the spindle pole body outer plaque; required forspindle orientation and mitotic nuclearmigration 0.0250.13318&radic&radic&radic S000000264ORC2YBR060CverifiedSubunit of the origin recognition complex, which directs DNAreplication by binding to replication originsand is also involved in transcriptionalsilencing; may be phosphorylated by Cdc28p 0.0250.13318&radic&radic S000001222OYE2YHR179WverifiedWidely conserved NADPH oxidoreductase containing flavinmononucleotide (FMN), homologous to Oye3p withslight differences in ligand binding andcatalytic properties; may be involved in sterolmetabolism 0.025010.13318&radic&radic&radic&radic&radic S000005738STE4YOR212WverifiedG protein beta subunit, forms a dimer with Ste18p to activatethe mating signaling pathway, forms aheterotrimer with Gpa1p and Ste18p to dampensignaling; may recruit Rho1p to the polarizedgrowth site during mating; contains WD40repeats 0.024940.13299&radic&radic&radic S000006058GIP3YPL137CverifiedGlc7-interacting protein whose overexpression relocalizes Glc7pfrom the nucleus and prevents chromosomesegregation 0.024930.13292&radic S000003421CRH1YGR189CverifiedPutative glycosidase of the cell wall, may have a role in cellwall architecture 0.02490.13288&radic S000004659BUB2YMR055CverifiedMitotic exit network regulator, forms GTPase-activatingBfa1p-Bub2p complex that binds Tem1p andspindle pole bodies, blocks cell cycleprogression before anaphase in response tospindle and kinetochore damage 0.024890.13273 S000006153SSO1YPL232WverifiedPlasma membrane t-SNARE involved in fusion of secretoryvesicles at the plasma membrane and in vesiclefusion during sporulation; forms a complex withSec9p that binds v-SNARE Snc2p; syntaxinhomolog; functionally redundant with Sso2p 0.024850.13266&radic&radic S000005031TCB2YNL087WverifiedBud-specific protein with a potential role in membranetrafficking; GFP-fusion protein migrates fromthe cell surface to intracellular vesicles nearvacuole; contains 3 calcium and lipid bindingdomains; mRNA is targeted to the bud via themRNA transport system involving She2p 0.024730.13135&radic S000005770ESA1YOR244WverifiedHistone acetyltransferase catalytic subunit of the nativemultisubunit complex (NuA4) that acetylatesfour conserved internal lysines of histone H4N-terminal tail; required for cell cycleprogression 0.024720.13135&radic&radic&radic S000006190KAR9YPL269WverifiedKaryogamy protein required for correct positioning of themitotic spindle and for orienting cytoplasmicmicrotubules, localizes at the shmoo tip inmating cells and at the tip of the growing budin small-budded cells through anaphase 0.024780.13135&radic&radic S000003973COF1YLL050CverifiedCofilin, promotes actin filament depolarization in apH-dependent manner; binds both actin monomersand filaments and severs filaments , thought tobe regulated by phosphorylation at SER4,ubiquitous and essential in eukaryotes 0.02480.13135&radic S000000213HHF1YBR009CverifiedOne of two identical histone H4 proteins (see also HHF2); corehistone required for chromatin assembly andchromosome function; contributes to telomericsilencing; N-terminal domain involved inmaintaining genomic integrity 0.024780.13135&radic S000005488YGK3YOL128CverifiedProtein kinase related to mammalian glycogen synthase kinasesof the GSK-3 family; GSK-3 homologs (Mck1p,Rim11p, Mrk1p, Ygk3p) are involved in controlof Msn2p-dependent transcription of stressresponsive genes and in protein degradation 0.02480.13135 S000005211PIK1YNL267WverifiedPhosphatidylinositol 4-kinase; catalyzes first step in thebiosynthesis ofphosphatidylinositol-4,5-biphosphate; maycontrol cytokineses through the actincytoskeleton 0.024620.13135&radic S000004584ATR1YML116WverifiedMultidrug efflux pump of the major facilitator superfamily,required for resistance to aminotriazole and4-nitroquinoline-N-oxide 0.024780.13135&radic S000003272KSS1YGR040WverifiedMitogen-activated protein kinase (MAPK) involved in signaltransduction pathways that control filamentousgrowth and pheromone response 0.024640.13135&radic&radic S000003587IRC8YJL051WuncharacterizedBud tip localized protein of unknown function; mRNA is targetedto the bud by a She2p dependent transportsystem; mRNA is cell cycle regulated via Fkh2p,peaking in G2/M phase; null mutant displaysincreased levels of spontaneous Rad52 foci 0.024590.13093 S000000301VPS15YBR097WverifiedMyristoylated serine/threonine protein kinase involved invacuolar protein sorting; functions as amembrane-associated complex with Vps34p; activeform recruits Vps34p to the Golgi membrane;interacts with the GDP-bound form of Gpa1p 0.024550.13093 S000000094PHO11YAR071WverifiedOne of three repressible acid phosphatases, a glycoprotein thatis transported to the cell surface by thesecretory pathway; induced by phosphatestarvation and coordinately regulated by PHO4and PHO2 0.024510.1308 S000002373PRR2YDL214CverifiedProtein kinase with a possible role in MAP kinase signaling inthe pheromone response pathway 0.024490.13068 S000002575CDC37YDR168WverifiedEssential Hsp90p co-chaperone; necessary for passage throughthe START phase of the cell cycle 0.024420.13029&radic&radic&radic&radic&radic S000002497YDR090CUncharacterizedHypothetical protein 0.024410.13029&radic&radic&radic S000001534SFK1YKL051WverifiedPlasma membrane protein that may act together with or upstreamof Stt4p to generate normal levels of theessential phospholipid PI4P, at least partiallymediates proper localization of Stt4p to theplasma membrane 0.024390.13026&radic&radic&radic&radic&radic S000005648PFY1YOR122CverifiedProfilin, actin- and phosphatidylinositol4,5-bisphosphate-binding protein, plays a rolein cytoskeleton organization, required fornormal timing of actin polymerization inresponse to thermal stress; localizes to plasmamembrane and cytosol &radic0.024360.13015&radic&radic&radic&radic&radic S000005327AGA1YNR044WverifiedAnchorage subunit of a-agglutinin of a-cells, highlyO-glycosylated protein with N-terminalsecretion signal and C-terminal signal foraddition of GPI anchor to cell wall, linked toadhesion subunit Aga2p via two disulfide bonds 0.024310.1299&radic S000003820PTK2YJR059WverifiedPutative serine/threonine protein kinase involved in regulationof ion transport across plasma membrane;enhances spermine uptake 0.024350.1299&radic S000003030PYC1YGL062WverifiedPyruvate carboxylase isoform, cytoplasmic enzyme that convertspyruvate to oxaloacetate; highly similar toisoform Pyc2p but differentially regulated;mutations in the human homolog are associatedwith lactic acidosis 0.024270.12972&radic&radic&radic S000002701SSD1YDR293CverifiedProtein with a role in maintenance of cellular integrity,interacts with components of the TOR pathway;ssd1 mutant of a clinical S. cerevisiae straindisplays elevated virulence 0.024280.12972&radic&radic&radic&radic&radic S000002794MUS81YDR386WverifiedHelix-hairpin-helix protein, involved in DNA repair andreplication fork stability; functions as anendonuclease in complex with Mms4p; interactswith Rad54p 0.024250.12946&radic&radic&radic S000005687PNS1YOR161CverifiedProtein of unknown function; has similarity to Torpedocalifornica tCTL1p, which is postulated to be acholine transporter, neither null mutation noroverexpression affects choline transport 0.024180.1291&radic&radic&radic S000002817SIZ1YDR409WverifiedSUMO ligase that promotes the attachment of sumo (Smt3p; smallubiquitin-related modifier) to proteins; bindsUbc9p and may bind septins; specificallyrequired for sumoylation of septins in vivo;localized to the septin ring 0.02420.1291&radic&radic&radic&radic&radic S000003593IKS1YJL057CuncharacterizedPutative serine/threonine kinase; expression is induced duringmild heat stress; deletion mutants arehypersensitive to copper sulphate and resistantto sorbate; interacts with an N-terminalfragment of Sst2p 0.024170.1291 S000003542CYR1YJL005WverifiedAdenylate cyclase, required for cAMP production andcAMP-dependent protein kinase signaling;involved in cell cycle control and glucose andnitrogen repression of sporulation 0.023960.12772&radic S000005979PDR12YPL058CverifiedPlasma membrane weak-acid-inducible ATP-binding cassette (ABC)transporter, required for weak organic acidresistance, strongly induced by sorbate andbenzoate, regulated by War1p, mutants exhibitsorbate hypersensitivity 0.023990.12772&radic&radic&radic S000000254REG2YBR050CverifiedRegulatory subunit of the Glc7p type-1 protein phosphatase;involved with Reg1p, Glc7p, and Snf1p inregulation of glucose-repressible genes, alsoinvolved in glucose-induced proteolysis ofmaltose permease 0.023970.12772 S000001308MET30YIL046WverifiedF-box protein containing five copies of the WD40 motif,controls cell cycle function, sulfurmetabolism, and methionine biosynthesis as partof the ubiquitin ligase complex; interacts withand regulates Met4p, localizes within thenucleus 0.02390.12768&radic&radic&radic S000005465WSC3YOL105CverifiedPartially redundant sensor-transducer of the stress-activatedPKC1-MPK1 signaling pathway involved inmaintenance of cell wall integrity; involved inthe response to heat shock and other stressors;regulates 1,3-beta-glucan synthesis 0.023810.12733&radic S000004879RSN1YMR266WverifiedMembrane protein of unknown function; overexpression suppressesNaCl sensitivity of sro7 mutant 0.023830.12733&radic&radic&radic S000005261PFS2YNL317WverifiedIntegral subunit of the pre-mRNA cleavage and polyadenylationfactor (CPF) complex; plays an essential rolein mRNA 3'-end formation by bridging differentprocessing factors and thereby promoting theassembly of the processing complex 0.023760.12706&radic&radic&radic S000005499CDC33YOL139CverifiedCytoplasmic mRNA cap binding protein; the eIF4E-cap complex isresponsible for mediating cap-dependent mRNAtranslation via interactions with thetranslation initiation factor eIF4G (Tif4631por Tif4632p) 0.023730.12685&radic S000001525SPC42YKL042WverifiedCentral plaque component of spindle pole body (SPB); involvedin SPB duplication, may facilitate attachmentof the SPB to the nuclear membrane 0.023670.1263&radic&radic&radic S000004190YKE2YLR200WverifiedSubunit of the heterohexameric Gim/prefoldin protein complexinvolved in the folding of alpha-tubulin,beta-tubulin, and actin 0.023680.1263&radic&radic&radic S000003769TDH2YJR009CverifiedGlyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involvedin glycolysis and gluconeogenesis; tetramerthat catalyzes the reaction ofglyceraldehyde-3-phosphate to 1,3bis-phosphoglycerate; detected in the cytoplasmand cell-wall 0.023650.1263&radic&radic S000001579CWP1YKL096WverifiedCell wall mannoprotein, linked to a beta-1,3- andbeta-1,6-glucan heteropolymer through aphosphodiester bond; involved in cell wallorganization 0.023650.1263&radic&radic&radic S000003933PSR1YLL010CverifiedPlasma membrane associated protein phosphatase involved in thegeneral stress response; required along withbinding partner Whi2p for full activation ofSTRE-mediated gene expression, possibly throughdephosphorylation of Msn2p 0.02360.12618&radic&radic&radic S000000165AST1YBL069WverifiedPeripheral membrane protein that interacts with the plasmamembrane ATPase Pma1p and has a role in itstargeting to the plasma membrane, possibly byinfluencing its incorporation into lipid rafts 0.023580.12609&radic&radic&radic&radic&radic S000003814BFA1YJR053WverifiedComponent of the GTPase-activating Bfa1p-Bub2p complex involvedin multiple cell cycle checkpoint pathways thatcontrol exit from mitosis 0.023510.12583 S000006157YPL236CUncharacterizedHypothetical protein 0.023510.12583&radic S000005153SSB2YNL209WverifiedCytoplasmic ATPase that is a ribosome-associated molecularchaperone, functions with J-protein partnerZuo1p; may be involved in the folding ofnewly-synthesized polypeptide chains; member ofthe HSP70 family; homolog of SSB1 0.023380.12516&radic S000000290IST2YBR086CverifiedPlasma membrane protein that may be involved in osmotolerance,localizes to the mother cell in small-buddedcells and to the bud in medium- andlarge-budded cells; mRNA is transported to thebud tip by an actomyosin-driven process 0.023380.12516&radic S000003616SCP160YJL080CverifiedEssential RNA-binding G protein effector of mating responsepathway, predominantly associated with nuclearenvelope and ER, interacts in mRNA-dependentmanner with translating ribosomes via multipleKH domains, similar to vertebrate vigilins 0.023420.12516 S000000283RPG1YBR079CverifiedSubunit of the core complex of translation initiation factor3(eIF3), essential for translation; part of asubcomplex (Prt1p-Rpg1p-Nip1p) that stimulatesbinding of mRNA and tRNA(i)Met to ribosomes 0.023370.12447&radic S000004240SSP120YLR250WverifiedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmin a punctate pattern 0.023280.12447&radic&radic&radic S000006082BEM4YPL161CverifiedProtein involved in establishment of cell polarity and budemergence; interacts with the Rho1p smallGTP-binding protein and with the Rho-typeGTPase Cdc42p; involved in maintenance ofproper telomere length 0.023270.12447&radic&radic&radic&radic S000004209MSC3YLR219WverifiedProtein of unknown function, green fluorescent protein(GFP)-fusion protein localizes to the cellperiphery; msc3 mutants are defective indirecting meiotic recombination events tohomologous chromatids; potential Cdc28psubstrate 0.023310.12447&radic&radic S000004069SIC1YLR079WverifiedInhibitor of Cdc28-Clb kinase complexes that controls G1/Sphase transition, preventing premature S phaseand ensuring genomic integrity; phosphorylationtargets Sic1p for SCF(CDC4)-dependent turnover;functional homolog of mammalian Kip1 0.023170.12375&radic&radic&radic&radic&radic S000003329ASK10YGR097WverifiedComponent of the RNA polymerase II holoenzyme, phosphorylatedin response to oxidative stress; has a role indestruction of Ssn8p, which relieves repressionof stress-response genes 0.02320.12375&radic&radic&radic&radic S000002128SBH1YER087C-BverifiedBeta subunit of the Sec61p ER translocation complex(Sec61p-Sss1p-Sbh1p); involved in proteintranslocation into the endoplasmic reticulum;interacts with the exocyst complex; homologousto Sbh2p 0.023190.12375&radic&radic S000000215IPP1YBR011CverifiedCytoplasmic inorganic pyrophosphatase (PPase), catalyzes therapid exchange of oxygens from Pi with water,highly expressed and essential for viability,active-site residues show identity to thosefrom E. coli PPase 0.02320.12375&radic&radic S000001052RPL27AYHR010WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl27Bp and has similarity to ratL27 ribosomal protein 0.023130.12354&radic&radic&radic S000002792ATO3YDR384CverifiedPlasma membrane protein, regulation pattern suggests a possiblerole in export of ammonia from the cell; memberof the TC 9.B.33 YaaH family of putativetransporters 0.023110.12354&radic&radic&radic&radic S000000322TEF2YBR118WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF1; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.023020.12297&radic S000000772GLY1YEL046CverifiedThreonine aldolase, catalyzes the cleavage of L-allo-threonineand L-threonine to glycine; involved in glycinebiosynthesis 0.022850.12198&radic&radic&radic&radic S000005630PIN2YOR104WverifiedProtein that induces appearance of [PIN+] prion whenoverproduced 0.022920.12198&radic&radic&radic S000006367TIF3YPR163CverifiedTranslation initiation factor eIF-4B, has RNA annealingactivity; contains an RNA recognition motif andbinds to single-stranded RNA 0.022890.12198&radic&radic&radic&radic&radic S000004828GAS3YMR215WverifiedPutative 1,3-beta-glucanosyltransferase, has similarity toGas1p; localizes to the cell wall 0.022940.12198&radic&radic&radic&radic S000000880YER078CUncharacterizedMetallopeptidase, localized to the mitochondrial matrix 0.022910.12198 S000005138YNL194CVerifiedIntegral membrane protein localized to eisosomes, large immobile protein structures at the cell cortex associated with endocytosis; sporulation and plasma membrane sphingolipid content are altered in mutants; has homologs SUR7 and FMP45 0.022890.12198&radic&radic&radic&radic S000003614PRY3YJL078CverifiedProtein of unknown function, has similarity to Pry1p and Pry2pand to the plant PR-1 class of pathogen relatedproteins 0.022750.12147 S000001711OSH6YKR003WverifiedMember of an oxysterol-binding protein family with overlapping,redundant functions in sterol metabolism andwhich collectively perform a function essentialfor viability; GFP-fusion protein localizes tothe cell periphery 0.022690.1211&radic&radic&radic S000000052GPB2YAL056WverifiedProposed beta subunit of the heterotrimeric G protein;interacts with the receptor Grp1p, affectssignaling by altering the level ofphosphorylation of PKS substrates; regulatespseudohyphal growth through cAMP levels;homolog of Gpb1p 0.022640.12082&radic&radic S000001767TIF1YKR059WverifiedTranslation initiation factor eIF4A, identical to Tif2p;DEA(D/H)-box RNA helicase that couples ATPaseactivity to RNA binding and unwinding; forms adumbbell structure of two compact domainsconnected by a linker; interacts with eIF4G 0.022620.12082&radic&radic S000006339CTF4YPR135WverifiedChromatin-associated protein, required for sister chromatidcohesion; interacts with DNA polymerase alpha(Pol1p) and may link DNA synthesis to sisterchromatid cohesion 0.022670.12082&radic&radic&radic S000000122LSM2YBL026WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.022610.12069&radic&radic&radic&radic&radic S000003977YLL054CUncharacterizedPutative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene 0.022470.11984&radic S000002936HLR1YDR528WverifiedProtein involved in regulation of cell wall composition andintegrity and response to osmotic stress;overproduction suppresses a lysis sensitive PKCmutation; similar to Lre1p, which functionsantagonistically to protein kinase A 0.022480.11984&radic S000005444PHM7YOL084WverifiedProtein of unknown function, expression is regulated byphosphate levels; green fluorescent protein(GFP)-fusion protein localizes to the cellperiphery and vacuole 0.022490.11984&radic&radic&radic&radic S000003834OPI3YJR073CverifiedPhospholipid methyltransferase(methylene-fatty-acyl-phospholipid synthase),catalyzes the last two steps inphosphatidylcholine biosynthesis 0.02250.11984 S000004399YLR407WUncharacterizedPutative protein of unknown function; YLR407W is not an essential gene 0.022490.11984&radic&radic S000002418SNQ2YDR011WverifiedABC transporter protein involved in multidrug resistance andresistance to singlet oxygen species 0.022420.11975&radic S000003617ARP4YJL081CverifiedNuclear actin-related protein involved in chromatin remodeling,component of chromatin-remodeling enzymecomplexes 0.02240.1194&radic S000004279MEC3YLR288CverifiedDNA damage and meiotic pachytene checkpoint protein; subunit ofa heterotrimeric complex (Rad17p-Mec3p-Ddc1p)that forms a sliding clamp, loaded onto partialduplex DNA by a clamp loader complex; homologof human and S. pombe Hus1 0.022420.1194&radic S000004267YSH1YLR277CverifiedPutative endonuclease, subunit of the mRNA cleavage andpolyadenylation specificity complex requiredfor 3' processing of mRNAs 0.022380.1194&radic&radic&radic S000000099HTA2YBL003CverifiedOne of two nearly identical (see also HTA1) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.022390.1194&radic&radic S000002820RRP17YDR412WuncharacterizedProtein required for cell viability 0.022390.1194&radic&radic&radic&radic&radic S000005212LYP1YNL268WverifiedLysine permease; one of three amino acid permeases (Alp1p,Can1p, Lyp1p) responsible for uptake ofcationic amino acids 0.022370.1194&radic&radic S000004692YMR086WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; YMR086W is not an essential gene; cAMP represses expression 0.022380.1194&radic S000002941HSP31YDR533CverifiedPossible chaperone and cysteine protease with similarity to E.coli Hsp31 and S. cerevisiae Hsp32p, Hsp33p,and Sno4p; member of the DJ-1/ThiJ/PfpIsuperfamily, which includes human DJ-1 involvedin Parkinson's disease; exists as a dimer 0.022320.11903&radic&radic S000001915FAB1YFR019Wverified1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membranekinase that generates phosphatidylinositol(3,5)P2, which is involved in vacuolar sortingand homeostasis 0.022310.11903 S000004372CSR1YLR380WverifiedPhosphatidylinositol transfer protein with a potential role inlipid turnover; interacts specifically withthioredoxin peroxidase (Tsa2p) and may have arole in oxidative stress resistance 0.022320.11903&radic&radic&radic&radic S000006342MEP3YPR138CverifiedAmmonium permease of high capacity and low affinity; belongs toa ubiquitous family of cytoplasmic membraneproteins that transport only ammonium (NH4+);expression is under the nitrogen cataboliterepression regulation ammonia permease 0.022320.11903&radic&radic&radic&radic&radic S000006284TEF1YPR080WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF2; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.022310.11903&radic S000003517ZUO1YGR285CverifiedCytosolic ribosome-associated chaperone that acts, togetherwith Ssz1p and the Ssb proteins, as a chaperonefor nascent polypeptide chains; contains a DnaJdomain and functions as a J-protein partner forSsb1p and Ssb2p 0.022270.11869&radic S000004186PWP1YLR196WverifiedProtein with WD-40 repeats involved in rRNA processing;associates with trans-acting ribosomebiogenesis factors; similar to beta-transducinsuperfamily 0.022280.11869&radic&radic&radic&radic S000004405YLR413WUncharacterizedPutative protein of unknown function; YLR413W is not an essential gene 0.022230.11869&radic S000006299SYT1YPR095CverifiedGuanine nucleotide exchange factor (GEF) for Arf proteins;involved in vesicular transport; suppressor ofypt3 mutations; member of the Sec7-domainfamily 0.022210.11866 S000000422PYC2YBR218CverifiedPyruvate carboxylase isoform, cytoplasmic enzyme that convertspyruvate to oxaloacetate; highly similar toisoform Pyc1p but differentially regulated;mutations in the human homolog are associatedwith lactic acidosis 0.022210.1185&radic&radic&radic&radic S000001415RRD1YIL153WverifiedActivator of the phosphotyrosyl phosphatase activity of PP2A;regulates G1 phase progression, the G2/M phasetransition, microtubule dynamics, theosmoresponse, bud morphogenesis and DNA repair;subunit of the Tap42p-Sit4p-Rrd1p complex 0.022140.11809&radic&radic S000000429YBR225WUncharacterizedHypothetical protein 0.022050.11771&radic&radic&radic S000002537FIN1YDR130CverifiedSpindle pole body-related intermediate filament protein, formscell cycle-specific filaments between spindlepole bodies in mother and daughter cells, ableto self-assemble, expression induced duringS/G2, localization cell-cycle dependent 0.021970.11716&radic&radic&radic&radic&radic S000003353MEP1YGR121CverifiedAmmonium permease; belongs to a ubiquitous family ofcytoplasmic membrane proteins that transportonly ammonium (NH4+); expression is under thenitrogen catabolite repression regulation 0.021960.11698&radic&radic S000003453TOS2YGR221CverifiedProtein involved in localization of Cdc24p to the site of budgrowth; may act as a membrane anchor; localizesto the bud neck and bud tip; potentiallyphosphorylated by Cdc28p &radic0.021880.11672&radic&radic&radic S000004825EFR3YMR212CverifiedNon-essential protein of unknown function; exhibits syntheticlethal genetic interactions with PHO85; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 0.021880.11664&radic S000005887PDE2YOR360CverifiedHigh-affinity cyclic AMP phosphodiesterase, component of thecAMP-dependent protein kinase signaling system,protects the cell from extracellular cAMP,contains readthrough motif surroundingtermination codon 0.02180.11545&radic&radic&radic&radic S000006360TPO3YPR156CverifiedPolyamine transport protein specific for spermine; localizes tothe plasma membrane; member of the majorfacilitator superfamily 0.021780.11545&radic&radic&radic S000000655YIH1YCR059CverifiedProtein that inhibits activation of Gcn2p, an eIF2 alphasubunit protein kinase, by competing for Gcn1pbinding, thus impacting gene expression inresponse to starvation; has sequence andfunctional similarity to the mouse IMPACT gene 0.021810.11545&radic&radic S000001190MRPL6YHR147CverifiedMitochondrial ribosomal protein of the large subunit 0.021720.11545&radic&radic&radic S000002320ENT1YDL161WverifiedEpsin-like protein involved in endocytosis and actin patchassembly and functionally redundant with Ent2p;binds clathrin via a clathrin-binding domainmotif at C-terminus 0.021690.11545 S000003701HAL5YJL165CverifiedPutative protein kinase; overexpression increases sodium andlithium tolerance, whereas gene disruptionincreases cation and low pH sensitivity andimpairs potassium uptake, suggesting a role inregulation of Trk1p and/or Trk2p transporters 0.021680.11545 S000003591YJL055WUncharacterizedPutative protein of unknown function; deletion mutants are sensitive to purine base analogs, 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA) and are AHA-hypermutable 0.021730.11545&radic&radic S000000953UBP3YER151CverifiedUbiquitin-specific protease that interacts with Bre5p toco-regulate anterograde and retrogradetransport between endoplasmic reticulum andGolgi compartments; inhibitor of genesilencing; cleaves ubiquitin fusions but notpolyubiquitin 0.021710.11545&radic S000001782YKR074WUncharacterizedPutative protein of unknown function; epitope-tagged protein localizes to the cytoplasm 0.021670.11545&radic&radic&radic&radic&radic&radic S000004920UBP15YMR304WverifiedUbiquitin-specific protease that may play a role in ubiquitinprecursor processing 0.021830.11545&radic&radic&radic S000004872YMR259CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR259C is not an essential gene 0.021760.11545&radic&radic&radic&radic&radic S000002482PPH3YDR075WverifiedCatalytic subunit of an evolutionarily conserved proteinphosphatase complex containing Psy2p and theregulatory subunit Psy4p; required forcisplatin resistance; involved in activation ofGln3p 0.02170.11545&radic&radic&radic&radic&radic S000006186DIP5YPL265WverifiedDicarboxylic amino acid permease, mediates high-affinity andhigh-capacity transport of L-glutamate andL-aspartate; also a transporter for Gln, Asn,Ser, Ala, and Gly 0.02170.11545&radic&radic S000004421CRN1YLR429WverifiedCoronin, cortical actin cytoskeletal component that associateswith the Arp2p/Arp3p complex to regulate itsactivity 0.021730.11545 S000003118INO80YGL150CverifiedATPase that forms a large complex, containing actin and severalactin-related proteins, that has chromatinremodeling activity and 3' to 5' DNA helicaseactivity in vitro; shows similarity to theSnf2p family of ATPases 0.02160.11468&radic S000005872YOR345CDubiousHypothetical protein 0.021540.11429 S000001595ABF1YKL112WverifiedDNA binding protein with possible chromatin-reorganizingactivity involved in transcriptionalactivation, gene silencing, and DNA replicationand repair 0.021530.11429&radic&radic&radic&radic S000001383QDR2YIL121WverifiedMultidrug transporter required for resistance to quinidine,barban, cisplatin, and bleomycin; member of themajor facilitator superfamily of transportersconferring multiple drug resistance (MFS-MDR) 0.021510.11429&radic S000001490CAP1YKL007WverifiedAlpha subunit of the capping protein (CP) heterodimer (Cap1pand Cap2p) which binds to the barbed ends ofactin filaments preventing furtherpolymerization; localized predominantly tocortical actin patches 0.021490.11429&radic&radic&radic&radic S000005256RFA2YNL312WverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 0.021430.11399&radic S000001332MAM33YIL070CverifiedAcidic protein of the mitochondrial matrix involved inoxidative phosphorylation; related to the humancomplement receptor gC1q-R 0.021450.11399 S000005441IRA2YOL081WverifiedGTPase-activating protein that negatively regulates RAS byconverting it from the GTP- to the GDP-boundinactive form, required for reducing cAMPlevels under nutrient limiting conditions, hassimilarity to Ira1p and human neurofibromin 0.021430.11399&radic&radic S000005086MEP2YNL142WverifiedAmmonium permease involved in regulation of pseudohyphalgrowth; belongs to a ubiquitous family ofcytoplasmic membrane proteins that transportonly ammonium (NH4+); expression is under thenitrogen catabolite repression regulation 0.02140.11378&radic S000005194RAD50YNL250WverifiedSubunit of MRX complex, with Mre11p and Xrs2p, involved inprocessing double-strand DNA breaks invegetative cells, initiation of meiotic DSBs,telomere maintenance, and nonhomologous endjoining 0.02130.11312&radic&radic S000003041HSF1YGL073WverifiedTrimeric heat shock transcription factor, activates multiplegenes in response to stresses that includehyperthermia; recognizes variable heat shockelements (HSEs) consisting of inverted NGAANrepeats; posttranslationally regulated 0.021320.11312&radic&radic&radic&radic&radic S000000129RIB1YBL033CverifiedGTP cyclohydrolase II; catalyzes the first step of theriboflavin biosynthesis pathway 0.021320.11312&radic&radic&radic S000000228SCO2YBR024WverifiedProtein anchored to the mitochondrial inner membrane, similarto Sco1p and may have a redundant function withSco1p in delivery of copper to cytochrome coxidase; interacts with Cox2p 0.021230.11281 S000002618YDR210WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 0.021250.11281&radic&radic&radic&radic S000002579SUP35YDR172WverifiedTranslation termination factor eRF3; altered proteinconformation creates the [PSI(+)] prion, adominant cytoplasmically inherited proteinaggregate that alters translational fidelityand creates a nonsense suppressor phenotype 0.02120.11259&radic&radic&radic&radic&radic S000006007ELP3YPL086CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;exhibits histone acetyltransferase activitythat is directed to histones H3 and H4;disruption confers resistance to K. lactiszymotoxin 0.021220.11259&radic&radic S000004470SPT5YML010WverifiedProtein that forms a complex with Spt4p and mediates bothactivation and inhibition of transcriptionelongation; Spt4p-Spt5p complex also plays arole in pre-mRNA processing 0.021160.11229&radic&radic&radic S000002385GCS1YDL226CverifiedADP-ribosylation factor GTPase activating protein (ARF GAP),involved in ER-Golgi transport; sharesfunctional similarity with Glo3p 0.021110.11214&radic S000001485DID4YKL002WverifiedClass E Vps protein of the ESCRT-III complex, required forsorting of integral membrane proteins intolumenal vesicles of multivesicular bodies, andfor delivery of newly synthesized vacuolarenzymes to the vacuole, involved in endocytosis 0.021130.11214&radic&radic&radic&radic S000002865TOM1YDR457WverifiedE3 ubiquitin ligase of the hect-domain class; has a role inmRNA export from the nucleus and may regulatetranscriptional coactivators 0.021080.11169&radic S000002522YDR115WUncharacterizedPutative mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins 0.020980.11138&radic&radic S000001676SDS22YKL193CverifiedConserved nuclear regulatory subunit of Glc7p type 1 proteinserine-threonine phosphatase (PP1), functionspositively with Glc7p to promotedephosphorylation of nuclear substratesrequired for chromosome transmission duringmitosis 0.020950.11102&radic&radic S000004938FET4YMR319CverifiedLow-affinity Fe(II) transporter of the plasma membrane 0.020920.11102&radic S000002869MFA1YDR461WverifiedMating pheromone a-factor, made by a cells; interacts withalpha cells to induce cell cycle arrest andother responses leading to mating; biogenesisinvolves C-terminal modification, N-terminalproteolysis, and export; also encoded by MFA2 0.020910.11102&radic&radic S000007234YDR034W-BUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 0.020960.11102&radic S000003107FLC3YGL139WverifiedPutative FAD transporter, similar to Flc1p and Flc2p; localizedto the ER 0.020910.11102&radic S000001391TAO3YIL129CverifiedProtein involved in cell morphogenesis and proliferation,associated with protein kinase Cbk1p; mutantsactivate OCH1 transcription 0.020910.11063&radic S000003581YJL045WUncharacterizedMinor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner 0.020850.11063 S000001132YNG2YHR090CverifiedSubunit of the NuA4 histone acetyltransferase complex thatacetylates histone H4 and H2A; has similarityto the human tumor suppressor ING1 0.020870.11063&radic S000001161SET1YHR119WverifiedHistone methyltransferase, subunit of the COMPASS (Set1C)complex which methylates histone H3 on lysine4; required in transcriptional silencing neartelomeres and at the silent mating type loci;contains a SET domain 0.020910.11063&radic&radic S000005133SRP1YNL189WverifiedKaryopherin alpha homolog, forms a dimer with karyopherin betaKap95p to mediate import of nuclear proteins,binds the nuclear localization signal of thesubstrate during import; may also play a rolein regulation of protein degradation 0.020870.11063&radic&radic&radic&radic S000004711PGM2YMR105CverifiedPhosphoglucomutase, catalyzes the conversion fromglucose-1-phosphate to glucose-6-phosphate,which is a key step in hexose metabolism;functions as the acceptor for aGlc-phosphotransferase 0.020880.11063&radic&radic S000001209CDC23YHR166CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition 0.020820.11043&radic S000003510CWC22YGR278WverifiedEssential protein, component of a complex containing Cef1p, hassimilarity to S. pombe Cwf22p; putativespliceosomal component, based on computationalanalysis of large-scale protein-proteininteraction data 0.02080.11032&radic&radic&radic S000002446ENA2YDR039CverifiedP-type ATPase sodium pump, involved in Na+ efflux to allow salttolerance; likely not involved in Li+ efflux 0.020740.10995&radic S000004675TVP18YMR071CverifiedIntegral membrane protein localized to late Golgi vesiclesalong with the v-SNARE Tlg2p 0.020740.10995 S000003209DOC1YGL240WverifiedProcessivity factor required for the ubiquitination activity ofthe anaphase promoting complex (APC), mediatesthe activity of the APC by contributing tosubstrate recognition; involved in cyclinproteolysis 0.020630.10929&radic&radic&radic&radic S000002187ARP2YDL029WverifiedEssential component of the Arp2/3 complex, which is a highlyconserved actin nucleation center required forthe motility and integrity of actin patches;involved in endocytosis and membrane growth andpolarity 0.020650.10929 S000005166SSU72YNL222WverifiedTranscription/RNA-processing factor that associates with TFIIBand cleavage/polyadenylation factor Pta1p;exhibits phosphatase activity on serine-5 ofthe RNA polymerase II C-terminal domain;affects start site selection in vivo 0.020610.10929&radic&radic S000005854SNC2YOR327CverifiedVesicle membrane receptor protein (v-SNARE) involved in thefusion between Golgi-derived secretory vesicleswith the plasma membrane; member of thesynaptobrevin/VAMP family of R-type v-SNAREproteins 0.020630.10929&radic&radic&radic S000000938GDI1YER136WverifiedGDP dissociation inhibitor, regulates vesicle traffic insecretory pathways by regulating thedissociation of GDP from the Sec4/Ypt/rabfamily of GTP binding proteins 0.020660.10929&radic S000000566MRC1YCL061CverifiedS-phase checkpoint protein found at replication forks, requiredfor DNA replication; also required for Rad53pactivation during DNA replication stress, whereit forms a replication-pausing complex withTof1p and is phosphorylated by Mec1p; proteininvolved in replication checkpoint 0.020690.10929&radic&radic&radic&radic S000000661HCM1YCR065WverifiedForkhead transcription factor that drives S-phase specificexpression of genes involved in chromosomesegregation, spindle dynamics, and budding;suppressor of calmodulin mutants with specificSPB assembly defects; telomere maintenance role 0.020650.10929&radic&radic S000002461CDC34YDR054CverifiedUbiquitin-conjugating enzyme or E2; together with Skp1p, Rbx1p,Cdc53p, and an F-box protein, forms aubiquitin-protein ligase called the SCF complexwhich regulates cell cycle progression bytargeting key substrates for degradation 0.020550.10896&radic&radic&radic&radic S000001160ORC6YHR118CverifiedSubunit of the origin recognition complex, which directs DNAreplication by binding to replication originsand is also involved in transcriptionalsilencing; may be phosphorylated by Cdc28p 0.020540.10896&radic&radic&radic&radic&radic&radic S000002641RTN1YDR233CverifiedER membrane protein that interacts with exocyst subunit Sec6pand with Yip3p; null mutant has an altered(mostly cisternal) ER morphology; hassimilarity to mammalian reticulon proteins andmember of the RTNLA (reticulon-like A)subfamily 0.020570.10896&radic&radic&radic S000005339BIO5YNR056CverifiedPutative transmembrane protein involved in the biotinbiosynthesis pathway; responsible for uptake of7-keto 8-aminopelargonic acid; BIO5 is in acluster of 3 genes (BIO3, BIO4, and BIO5) thatmediate biotin synthesis 0.020580.10896&radic S000000024DRS2YAL026CverifiedAminophospholipid translocase (flippase) that maintainsmembrane lipid asymmetry in post-Golgisecretory vessicles; localizes to thetrans-Golgi network; contributes toclathrin-coated vesicle formation andendocytosis; type 4 P-type ATPase 0.020510.1088&radic S000004610PLB1YMR008CverifiedPhospholipase B (lysophospholipase) involved in lipidmetabolism, required for deacylation ofphosphatidylcholine andphosphatidylethanolamine but notphosphatidylinositol 0.020440.10848&radic&radic S000003815YJR054WUncharacterizedVacuolar protein of unknown function; potential Cdc28p substrate 0.02050.10848