Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0006265 "DNA topological change"

GO branch: BP
Number of currently annotated genes: 7

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest Parsons:CamptothecinGiaever:methotrexate_125uMHo:S000005475Giaever:cisplatin_66uMGiaever:cisplatin_500uMGiaever:cisplatin_250uMHarbison:GAL4_GALHarbison:AFT2_YPDHarbison:STB5_YPDHo:S000002794 S000005943RAD1YPL022WverifiedSingle-stranded DNA endonuclease (with Rad10p), cleavessingle-stranded DNA during nucleotide excisionrepair and double-strand break repair; subunitof Nucleotide Excision Repair Factor 1 (NEF1);homolog of human XPF protein 0.139470.85521&radic&radic&radic S000005243TRF5YNL299WverifiedPoly (A) polymerase involved in nuclear RNA quality controlbased on: homology with Trf4p, geneticinteractions with TRF4 mutants, physicalinteraction with Mtr4p (TRAMP subunit), and bydirect assay; disputed role as a sigma DNApolymerase 0.090520.73925 S000000303YBR099CDubiousHypothetical protein 0.074310.67982&radic&radic&radic&radic&radic&radic S000004560RAD10YML095CverifiedSingle-stranded DNA endonuclease (with Rad1p), cleavessingle-stranded DNA during nucleotide excisionrepair and double-strand break repair; subunitof Nucleotide Excision Repair Factor 1 (NEF1);homolog of human ERCC1 protein 0.064910.65392&radic&radic&radic&radic S000005598YOR072WDubiousHypothetical protein 0.041470.56375&radic&radic&radic S000002625RAD9YDR217CverifiedDNA damage-dependent checkpoint protein, required forcell-cycle arrest in G1/S, intra-S, and G2/M;transmits checkpoint signal by activatingRad53p and Chk1p; hyperphosphorylated by Mec1pand Tel1p; potential Cdc28p substrate 0.038730.55351&radic&radic&radic&radic S000002794MUS81YDR386WverifiedHelix-hairpin-helix protein, involved in DNA repair andreplication fork stability; functions as anendonuclease in complex with Mms4p; interactswith Rad54p &radic0.033610.53429&radic&radic&radic&radic&radic&radic&radic S000004022RAD5YLR032WverifiedSingle-stranded DNA-dependent ATPase, involved inpostreplication repair; contains RING fingerdomain 0.022150.46124&radic&radic&radic&radic&radic S000005895RAD17YOR368WverifiedCheckpoint protein, involved in the activation of the DNAdamage and meiotic pachytene checkpoints; withMec3p and Ddc1p, forms a clamp that is loadedonto partial duplex DNA; homolog of human andS. pombe Rad1 and U. maydis Rec1 proteins 0.019750.43592&radic&radic&radic S000002483RAD55YDR076WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad57p 0.018570.42456&radic&radic&radic&radic S000003586MTR4YJL050WverifiedDead-box family ATP dependent helicase required for mRNA exportfrom the nucleus; co-factor of the exosomecomplex, required for 3' end formation of 5.8SrRNA 0.014330.37939 S000005308YNR025CDubiousHypothetical protein 0.013030.36731&radic&radic S000004279MEC3YLR288CverifiedDNA damage and meiotic pachytene checkpoint protein; subunit ofa heterotrimeric complex (Rad17p-Mec3p-Ddc1p)that forms a sliding clamp, loaded onto partialduplex DNA by a clamp loader complex; homologof human and S. pombe Hus1 0.011950.35353&radic&radic&radic&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p 0.011580.35187&radic&radic S000000302MMS4YBR098WverifiedSubunit of the structure-specific Mms4p-Mus81p endonucleasethat cleaves branched DNA; involved inrecombination and DNA repair &radic0.010010.33176&radic&radic&radic&radic&radic&radic&radic&radic S000000231YBR027CDubiousHypothetical protein 0.009250.32202&radic&radic&radic&radic S000000682CSM1YCR086WverifiedNucleolar protein that forms a complex with Lrs4p which bindsMam1p at kinetochores during meiosis I tomediate accurate chromosome segregation, may beinvolved in premeiotic DNA replication;possible role in telomere maintenance 0.008070.30716&radic&radic&radic S000002638YDR230WDubiousHypothetical protein 0.007890.30332&radic&radic&radic&radic S000004312MMS22YLR320WverifiedProtein involved in resistance to ionizing radiation; acts withMms1p in a repair pathway that may be involvedin resolving replication intermediates orpreventing the damage caused by blockedreplication forks 0.007520.29682&radic&radic S000000975RAD24YER173WverifiedCheckpoint protein, involved in the activation of the DNAdamage and meiotic pachytene checkpoints;subunit of a clamp loader that loadsRad17p-Mec3p-Ddc1p onto DNA; homolog of humanand S. pombe Rad17 protein 0.006650.27452&radic&radic&radic S000005176CSL4YNL232WverifiedSubunit of the exosome, which is an essential complex presentin both nucleus and cytoplasm that mediates RNAprocessing and degradation 0.006550.27152 S000003391NSR1YGR159CverifiedNucleolar protein that binds nuclear localization sequences,required for pre-rRNA processing and ribosomebiogenesis 0.006130.25982 S000004846YMR233WUncharacterizedProtein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; YMR233W is not an essential gene 0.005590.24369&radic&radic S000005366TOP1YOL006CverifiedTopoisomerase I, nuclear enzyme that relieves torsional strainin DNA by cleaving and re-sealing thephosphodiester backbone; relaxes bothpositively and negatively supercoiled DNA;functions in replication, transcription, andrecombination &radic0.004760.21205 S000000566MRC1YCL061CverifiedS-phase checkpoint protein found at replication forks, requiredfor DNA replication; also required for Rad53pactivation during DNA replication stress, whereit forms a replication-pausing complex withTof1p and is phosphorylated by Mec1p; proteininvolved in replication checkpoint 0.004620.20538 S000002777XRS2YDR369CverifiedProtein required for DNA repair; component of the Mre11complex, which is involved in double strandbreaks, meiotic recombination, telomeremaintenance, and checkpoint signaling 0.004560.2036&radic&radic&radic&radic S000004187SIK1YLR197WverifiedEssential evolutionarily-conserved nucleolar protein componentof the box C/D snoRNP complexes that direct2'-O-methylation of pre-rRNA during itsmaturation; overexpression causes spindleorientation defects 0.004110.18386 S000002847LRS4YDR439WverifiedProtein involved in rDNA silencing; positively chargedcoiled-coil protein with limited similarity tomyosin 0.003710.16801&radic S000001710PAP1YKR002WverifiedPoly(A) polymerase, one of three factors required for mRNA3'-end polyadenylation, forms multiproteincomplex with polyadenylation factor I (PF I),also required for mRNA nuclear export; may alsopolyadenylate rRNAs 0.003550.1606 S000000427TDP1YBR223CverifiedTyrosyl-DNA Phosphodiesterase I, hydrolyzes 3'-phosphotyrosylbonds to generate 3'-phosphate DNA andtyrosine, involved in the repair of DNA lesionscreated by topoisomerase I 0.003490.15825 S000001056SPO13YHR014WverifiedMeiosis-specific protein, involved in maintaining sisterchromatid cohesion during meiosis I as well aspromoting proper attachment of kinetochores tothe spindle during meiosis I and meiosis II 0.003330.15292 S000002259DUN1YDL101CverifiedCell-cycle checkpoint serine-threonine kinase required for DNAdamage-induced transcription of certain targetgenes, phosphorylation of Rad55p and Sml1p, andtransient G2/M arrest after DNA damage; alsoregulates postreplicative DNA repair 0.003290.15152&radic&radic S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 0.003260.15036&radic&radic S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.003210.14878 S000000203YBL107CUncharacterizedHypothetical protein 0.003220.14878&radic&radic&radic S000000631RRP43YCR035CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp4p and Dis3p; required for efficientmaturation of 5.8S, 18S and 25S rRNA 0.002990.13849 S000005076KRE33YNL132WverifiedEssential protein of unknown function; heterozygous mutantshows haploinsufficiency in K1 killer toxinresistance 0.002960.13781 S000001176WSS1YHR134WverifiedSumoylated protein of unknown function, identified based ongenetic interactions with SMT3; UV-sensitivemutant phenotype and genetic interactionssuggest a role in the DNA damage response,processing stalled or collapsed replicationforks 0.002960.13781 S000006074RAD53YPL153CverifiedProtein kinase, required for cell-cycle arrest in response toDNA damage; activated by transautophosphorylation when interacting withhyperphosphorylated Rad9p; also interacts withARS1 and plays a role in initiation of DNAreplication 0.00290.13609 S000002770TFC6YDR362CverifiedOne of six subunits of RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part ofTFIIIC TauB domain that binds BoxB promotersites of tRNA and other genes; cooperates withTfc3p in DNA binding; human homolog isTFIIIC-110 0.002840.13328 S000004186PWP1YLR196WverifiedProtein with WD-40 repeats involved in rRNA processing;associates with trans-acting ribosomebiogenesis factors; similar to beta-transducinsuperfamily 0.002820.13228 S000005401NOP12YOL041CverifiedNucleolar protein, required for pre-25S rRNA processing;contains an RNA recognition motif (RRM) and hassimilarity to Nop13p, Nsr1p, and putativeorthologs in Drosophila and S. pombe 0.00280.13146 S000003322UTP22YGR090WuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.00280.13146 S000003209DOC1YGL240WverifiedProcessivity factor required for the ubiquitination activity ofthe anaphase promoting complex (APC), mediatesthe activity of the APC by contributing tosubstrate recognition; involved in cyclinproteolysis 0.002780.13036&radic&radic S000005252KRI1YNL308CverifiedEssential nucleolar protein required for 40S ribosomebiogenesis; physically and functionallyinteracts with Krr1p 0.002740.12918 S000005381DIS3YOL021CverifiedNucleolar exosome component, involved in rRNA processing andRNA degradation, binds Gsp1p/Ran and enhancesthe GEF activity of Srm1p, implicated inmitotic control, homologous to the E. coliRNase R of the RNase II family 0.002740.12918 S000005005NOP2YNL061WverifiedProbable RNA m(5)C methyltransferase, essential for processingand maturation of 27S pre-rRNA and largeribosomal subunit biogenesis; localized to thenucleolus; constituent of 66S pre-ribosomalparticles 0.002720.12844 S000004651CSM3YMR048WverifiedProtein required for accurate chromosome segregation duringmeiosis 0.00270.12745&radic&radic S000002367NHP2YDL208WverifiedNuclear protein related to mammalian high mobility group (HMG)proteins, essential for function of H/ACA-typesnoRNPs, which are involved in 18S rRNAprocessing 0.002690.12653 S000002980ERG4YGL012WverifiedC-24(28) sterol reductase, catalyzes the final step inergosterol biosynthesis; mutants are viable,but lack ergosterol 0.002670.12581&radic&radic&radic&radic S000001293ULP2YIL031WverifiedPeptidase that deconjugates Smt3/SUMO-1 peptides from proteins,plays a role in chromosome cohesion atcentromeric regions and recovery fromcheckpoint arrest induced by DNA damage or DNAreplication defects; potential Cdc28p substrate 0.002660.12581 S000003628HPR5YJL092WverifiedDNA helicase and DNA-dependent ATPase involved in DNA repair,required for proper timing of commitment tomeiotic recombination and the transition fromMeiosis I to Meiosis II; potential Cdc28psubstrate 0.002590.12266&radic S000004652ERB1YMR049CverifiedProtein required for maturation of the 25S and 5.8S ribosomalRNAs; constituent of 66S pre-ribosomalparticles; homologous to mammalian Bop1 0.002580.12266 S000003088PRP43YGL120CverifiedRNA helicase in the DEAH-box family, functions in both RNApolymerase I and polymerase II transcriptmetabolism, involved in release of thelariat-intron from the spliceosome 0.002570.12217 S000002217RAD59YDL059CverifiedProtein involved in the repair of double-strand breaks in DNAduring vegetative growth via recombination andsingle-strand annealing; anneals complementarysingle-stranded DNA; homologous to Rad52p 0.002520.11984&radic&radic&radic&radic S000000808NUG1YER006WverifiedGTPase that associates with nuclear 60S pre-ribosomes, requiredfor export of 60S ribosomal subunits from thenucleus 0.002450.11754 S000001789RPF2YKR081CverifiedEssential protein involved in the processing of pre-rRNA andthe assembly of the 60S ribosomal subunit;interacts with ribosomal protein L11; localizespredominantly to the nucleolus; constituent of66S pre-ribosomal particles 0.002440.1164 S000003026RAD6YGL058WverifiedUbiquitin-conjugating enzyme (E2), involved in postreplicationrepair (with Rad18p), sporulation, telomeresilencing, and ubiquitin-mediated N-end ruleprotein degradation (with Ubr1p) 0.002410.11571 S000001897LOC1YFR001WverifiedNuclear protein involved in asymmetric localization of ASH1mRNA; binds double-stranded RNA in vitro;constituent of 66S pre-ribosomal particles 0.002420.11571 S000003684RPA34YJL148WverifiedRNA polymerase I subunit A34.5 0.00240.11538 S000002563RPA14YDR156WverifiedRNA polymerase I subunit A14 0.002390.11476&radic S000004539FPR3YML074CverifiedNucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506binding protein; phosphorylated by caseinkinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) anddephosphorylated by Ptp1p 0.002370.11425 S000001132YNG2YHR090CverifiedSubunit of the NuA4 histone acetyltransferase complex thatacetylates histone H4 and H2A; has similarityto the human tumor suppressor ING1 0.002350.11403 S000001185RPC10YHR143W-AverifiedRNA polymerase subunit, found in RNA polymerase complexes I,II, and III 0.002320.1126 S000003061SPC105YGL093WverifiedProtein required for accurate chromosome segregation, localizesto the nuclear side of the spindle pole body;forms a complex with Ydr532cp 0.002330.1126 S000006345KAR3YPR141CverifiedMinus-end-directed microtubule motor that functions in mitosisand meiosis, localizes to the spindle pole bodyand localization is dependent on functionalCik1p, required for nuclear fusion duringmating; potential Cdc28p substrate 0.002320.11222&radic S000002313MSH5YDL154WverifiedProtein of the MutS family, forms a dimer with Msh4p thatfacilitates crossovers between homologs duringmeiosis; msh5-Y823H mutation confers toleranceto DNA alkylating agents; homologs present inC. elegans and humans 0.002260.11008&radic&radic S000001441MPH1YIR002CverifiedMember of the DEAH family of helicases, functions in anerror-free DNA damage bypass pathway thatinvolves homologous recombination, mutationsconfer a mutator phenotype 0.002250.10971&radic&radic S000001305CBR1YIL043CverifiedMicrosomal cytochrome b reductase, not essential for viability;also detected in mitochondria; mutation inconserved NADH binding domain of the humanortholog results in type I methemoglobinemia 0.002250.10971&radic&radic&radic S000003131RAD54YGL163CverifiedDNA-dependent ATPase, stimulates strand exchange by modifyingthe topology of double-stranded DNA; involvedin the recombinational repair of double-strandbreaks in DNA during vegetative growth andmeiosis; member of the SWI/SNF family 0.00220.10746&radic S000000229YBR025CUncharacterizedHypothetical protein 0.002170.10659&radic&radic&radic S000002198NAT1YDL040CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.002050.10084&radic S000002334AIR2YDL175CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p; may regulatemethylation of Npl3p, which modulates Npl3pfunction in mRNA processing and export; hassimilarity to Air1p 0.002050.10084 S000005475PAP2YOL115WverifiedCatalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), anuclear poly (A) polymerase complex involved inRNA quality control; catalyzes polyadenylationof unmodified tRNAs, and snoRNA and rRNAprecursors; disputed role as a DNA polymerase &radic0.001940.09649&radic S000005217TOF1YNL273WverifiedSubunit of a replication-pausing checkpoint complex(Tof1p-Mrc1p-Csm3p) that acts at the stalledreplication fork to promote sister chromatidcohesion after DNA damage, facilitating gaprepair of damaged DNA; interacts with the MCMhelicase &radic0.001840.0924&radic&radic&radic&radic S000005032TOP2YNL088WverifiedEssential type II topoisomerase, relieves torsional strain inDNA by cleaving and re-sealing thephosphodiester backbone of both positively andnegatively supercoiled DNA; cleavescomplementary strands; localizes to axial coresin meiosis &radic0.001820.09036 S000002674YDR266CUncharacterizedHypothetical protein 0.001650.08329 S000005206POL2YNL262WverifiedCatalytic subunit of DNA polymerase epsilon, one of the majorchromosomal DNA replication polymerasescharacterized by processivity and proofreadingexonuclease activity; also involved in DNAsynthesis during DNA repair 0.001640.08262 S000001014SPO11YHL022CverifiedMeiosis-specific protein that initiates meiotic recombinationby catalyzing the formation of double-strandbreaks in DNA via a transesterificationreaction; required for homologous chromosomepairing and synaptonemal complex formation 0.001620.08025 S000004844PEP5YMR231WverifiedPeripheral vacuolar membrane protein required for proteintrafficking and vacuole biogenesis; formscomplex with Pep3p that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, also interacts with Pep7p 0.00160.08025&radic&radic S000006347RRP15YPR143WverifiedNucleolar protein, constituent of pre-60S ribosomal particles;required for processing of the 27S pre-rRNA atthe A2 site to yield 5.8S and 25S rRNA 0.001550.07784&radic S000005872YOR345CDubiousHypothetical protein 0.001480.07434&radic&radic&radic S000003824RPA12YJR063WverifiedRNA polymerase I subunit A12.2; contains two zinc bindingdomains, and the N terminal domain isresponsible for anchoring to the RNA pol Icomplex 0.001480.07434&radic S000002742SWR1YDR334WverifiedSwi2/Snf2-related ATPase that is the structural component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.001440.07267&radic S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus 0.001430.07248&radic&radic&radic S000006323CLB2YPR119WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 0.001430.0721&radic&radic S000005762DFR1YOR236WverifiedDihydrofolate reductase, part of the dTTP biosynthetic pathway,involved in folate metabolism, possiblyrequired for mitochondrial function 0.001420.0721&radic S000004093CDC45YLR103CverifiedDNA replication initiation factor; recruited to MCM pre-RCcomplexes at replication origins; promotesrelease of MCM from Mcm10p, recruits elongationmachinery; mutants in human homolog may causevelocardiofacial and DiGeorge syndromes 0.001420.0721&radic S000005670ELG1YOR144CverifiedProtein required for S phase progression and telomerehomeostasis, forms an alternative replicationfactor C complex important for DNA replicationand genome integrity; involved in homologousrecombination-mediated DNA repair 0.00140.0711 S000004682VPS20YMR077CverifiedMyristoylated subunit of ESCRTIII, the endosomal sortingcomplex required for transport of transmembraneproteins into the multivesicular body pathwayto the lysosomal/vacuolar lumen; cytoplasmicprotein recruited to endosomal membranes 0.001380.07&radic S000002269RRP42YDL111CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp4p, Rrp41p,Rrp43p and Dis3p 0.001390.07&radic&radic S000000607POL4YCR014CverifiedDNA polymerase IV, undergoes pair-wise interactions withDnl4p-Lif1p and Rad27p to mediate repair of DNAdouble-strand breaks by non-homologous endjoining (NHEJ); homologous to mammalian DNApolymerase beta 0.001390.07 S000006373JIP5YPR169WuncharacterizedNucleolar protein of unknown function, exhibits a physicalinteraction with Bre1p 0.001370.06959&radic&radic&radic S000006346YPR142CDubiousHypothetical protein; open reading frame overlaps 5' end of essential RRP15 gene required for ribosomal RNA processing 0.001360.06888&radic&radic S000004561YML094C-ADubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W 0.001360.06888&radic&radic S000005210YNL266WDubiousHypothetical protein 0.001360.06888&radic&radic S000001594YKL111CDubiousDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1 0.001360.06888&radic&radic S000003798YJR037WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; YJR037W is not an essential gene. 0.001360.06888&radic&radic&radic S000001316YIL054WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data 0.001360.06888&radic&radic S000003086YGL118CDubiousHypothetical protein 0.001360.06888&radic&radic&radic S000000563FYV5YCL058CdubiousProtein of unknown function, required for survival uponexposure to K1 killer toxin; involved in ionhomeostasis 0.001360.06888&radic&radic S000003451YGR219WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C 0.001360.06888&radic&radic S000002311YDL152WDubiousHypothetical protein; open reading frame overlaps 5' end of essential SAS10 gene encoding a component of the small ribosomal subunit processosome 0.001360.06888&radic&radic&radic S000001566YKL083WDubiousDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 0.001360.06888&radic&radic&radic S000000173YBL077WDubiousHypothetical protein; open reading frame overlaps 3' end of essential ILS1 gene encoding cytoplasmic isoleucine-tRNA synthetase 0.001360.06888&radic&radic&radic S000002208YDL050CDubiousHypothetical protein 0.001360.06888&radic&radic S000002184YDL026WDubiousHypothetical protein 0.001360.06888&radic&radic S000004450YLR458WDubiousHypothetical protein; open reading frame overlaps 5' end of essential NBP1 gene required for mitosis 0.001360.06888&radic&radic&radic S000003957RIX7YLL034CverifiedPutative ATPase of the AAA family, required for export ofpre-ribosomal large subunits from the nucleus;distributed between the nucleolus, nucleoplasm,and nuclear periphery depending on growthconditions 0.001360.06888&radic&radic S000003347YGR115CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C 0.001360.06888&radic&radic S000005439YOL079WDubiousHypothetical protein 0.001360.06888&radic&radic S000000158YBL062WDubiousHypothetical protein 0.001360.06888&radic&radic&radic S000003346YGR114CDubiousHypothetical protein; open reading frame overlaps 5' end of essential SPT6 gene involved in nucleosome disassembly and reassembly during transcription 0.001360.06888&radic&radic S000005459YOL099CDubiousHypothetical protein 0.001360.06888&radic&radic S000002459DBF4YDR052CverifiedRegulatory subunit of Cdc7p-Dbf4p kinase complex, required forCdc7p kinase activity and initiation of DNAreplication; phosphorylates the Mcm2-7 familyof proteins; cell cycle regulated 0.001360.0686 S000005466YOL106WDubiousHypothetical protein 0.001350.06794&radic&radic S000004436YLR444CDubiousHypothetical protein 0.001350.06794&radic&radic S000005725YOR199WDubiousHypothetical protein 0.001350.06794&radic&radic S000005200FOL1YNL256WverifiedMultifunctional enzyme of the folic acid biosynthesis pathway,has dihydropteroate synthetase,dihydro-6-hydroxymethylpterinpyrophosphokinase, and dihydroneopterinaldolase activities 0.001360.06794&radic&radic S000005726YOR200WDubiousHypothetical protein 0.001350.06794&radic&radic S000001188YHR145CDubiousHypothetical protein 0.001350.06794&radic&radic&radic S000002494RRP1YDR087CverifiedEssential evolutionarily conserved nucleolar protein necessaryfor biogenesis of 60S ribosomal subunits andprocessing of pre-rRNAs to mature rRNAs,associated with several distinct 66Spre-ribosomal particles 0.001360.06794&radic&radic&radic S000003491YGR259CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W 0.001350.06794&radic&radic&radic S000003600YJL064WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 0.001350.06794&radic&radic S000004330OPI9YLR338WdubiousDubious open reading frame unlikely to encode a protein, basedon available experimental and comparativesequence data; partially overlaps the verifiedORF VRP1/YLR337C 0.001360.06794&radic&radic S000000683YCR087WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene 0.001320.06609&radic&radic&radic S000002254OPI6YDL096CdubiousDubious open reading frame unlikely to encode a protein, basedon available experimental and comparativesequence data; partially overlaps verified genePMT1/YDL095W; YDL096C is not essential 0.001320.06609&radic&radic&radic S000001596RAD27YKL113Cverified5' to 3' exonuclease, 5' flap endonuclease, required forOkazaki fragment processing and maturation aswell as for long-patch base-excision repair;member of the S. pombe RAD2/FEN1 family 0.001330.06609 S000005606DIA2YOR080WverifiedOrigin-binding F-box protein that forms an SCF ubiquitin ligasecomplex with Skp1p and Cdc53p; plays a role inDNA replication, involved in invasive andpseudohyphal growth 0.001310.0659 S000005942ECM23YPL021WverifiedNon-essential protein of unconfirmed function; affects pre-rRNAprocessing, may act as a negative regulator ofthe transcription of genes involved inpseudohyphal growth; homologous to Srd1p 0.00130.06523&radic S000005552BUB3YOR026WverifiedKinetochore checkpoint WD40 repeat protein that localizes tokinetochores during prophase and metaphase,delays anaphase in the presence of unattachedkinetochores; forms complexes with Mad1p-Bub1pand with Cdc20p, binds Mad2p and Mad3p 0.00130.06413 S000004577ZDS2YML109WverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; paralogof Zds1p 0.001290.06413 S000004494RAD52YML032CverifiedProtein that stimulates strand exchange by facilitating Rad51pbinding to single-stranded DNA; annealscomplementary single-stranded DNA; involved inthe repair of double-strand breaks in DNAduring vegetative growth and meiosis 0.001290.06413&radic S000004558UTP14YML093WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.001250.06194&radic&radic S000003971YBT1YLL048CverifiedTransporter of the ATP-binding cassette (ABC) family involvedin bile acid transport; similar to mammalianbile transporters 0.001250.06194&radic S000005945RMI1YPL024WverifiedInvolved in response to DNA damage; null mutants have increasedrates of recombination and delayed S phase;interacts physically and genetically with Sgs1p(RecQ family member) and Top3p (topoisomeraseIII) 0.001260.06194 S000004172SWI6YLR182WverifiedTranscription cofactor, forms complexes with DNA-bindingproteins Swi4p and Mbp1p to regulatetranscription at the G1/S transition; involvedin meiotic gene expression; localizationregulated by phosphorylation; potential Cdc28psubstrate 0.001250.06194 S000000763RAD23YEL037CverifiedProtein with ubiquitin-like N terminus, recognizes and bindsdamaged DNA (with Rad4p) during nucleotideexcision repair; regulates Rad4p levels,subunit of Nuclear Excision Repair Factor 2(NEF2); homolog of human HR23A and HR23Bproteins 0.001240.06151&radic&radic&radic S000000176PET112YBL080CverifiedProtein required for mitochondrial translation; mutation isfunctionally complemented by a Bacillussubtilis ortholog 0.001220.06046&radic&radic S000005335POP2YNR052CverifiedRNase of the DEDD superfamily, subunit of the Ccr4-Not complexthat mediates 3' to 5' mRNA deadenylation 0.001220.06046&radic S000000540GRX1YCL035CverifiedHydroperoxide and superoxide-radical responsive heat-stableglutathione-dependent disulfide oxidoreductasewith active site cysteine pair; protects cellsfrom oxidative damage 0.001230.06046&radic S000004390SKI2YLR398CverifiedPutative RNA helicase, involved in exosome mediated 3' to 5'mRNA degradation and translation inhibition ofnon-poly(A) mRNAs; forms complex with Ski3p andSki8p; required for repressing propagation ofdsRNA viruses 0.001210.05959 S000001052RPL27AYHR010WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl27Bp and has similarity to ratL27 ribosomal protein 0.001210.05959&radic&radic&radic S000003802URB2YJR041CverifiedNucleolar protein required for normal metabolism of the rRNAprimary transcript, proposed to be involved inribosome biogenesis 0.00120.05899&radic S000005844FAA1YOR317WverifiedLong chain fatty acyl-CoA synthetase with a preference forC12:0-C16:0 fatty acids; involved in theactivation of imported fatty acids; localizedto both lipid particles and mitochondrial outermembrane; essential for stationary phase 0.001190.05886&radic S000002789YRA1YDR381WverifiedNuclear protein that binds to RNA and to Mex67p, required forexport of poly(A)+ mRNA from the nucleus;member of the REF (RNA and export factorbinding proteins) family; another familymember, Yra2p, can substitute for Yra1pfunction 0.001170.05774 S000000063NUP60YAR002WverifiedSubunit of the nuclear pore complex (NPC), functions to anchorNup2p to the NPC in a process controlled by thenucleoplasmic concentration of Gsp1p-GTP;potential Cdc28p substrate; involved intelomere maintenance 0.001140.05577&radic S000004328SGD1YLR336CverifiedEssential nuclear protein with a possible role in theosmoregulatory glycerol response; interactswith phospholipase C (Plc1p); putative homologof human NOM1 which is implicated in acutemyeloid leukemia 0.001140.05577&radic&radic S000000351YBR147WUncharacterizedPutative protein of unknown function; YBR147W is not an essential gene; resistant to fluconazole 0.001140.05512&radic S000002482PPH3YDR075WverifiedCatalytic subunit of an evolutionarily conserved proteinphosphatase complex containing Psy2p and theregulatory subunit Psy4p; required forcisplatin resistance; involved in activation ofGln3p 0.001130.05512&radic&radic S000004802SGS1YMR190CverifiedNucleolar DNA helicase of the RecQ family involved inmaintenance of genome integrity, regulateschromosome synapsis and meiotic crossing over;has similarity to human BLM and WRN helicasesimplicated in Bloom and Werner syndromes 0.001130.05466&radic S000004224TOP3YLR234WverifiedDNA Topoisomerase III, conserved protein that functions in acomplex with Sgs1p and Rmi1p to relaxsingle-stranded negatively-supercoiled DNApreferentially, involved in telomere stabilityand regulation of mitotic recombination 0.001120.05466&radic S000005056DBP2YNL112WverifiedEssential ATP-dependent RNA helicase of the DEAD-box proteinfamily, involved in nonsense-mediated mRNAdecay and rRNA processing 0.001120.05428 S000006379DPB2YPR175WverifiedSecond largest subunit of DNA polymerase II (DNA polymeraseepsilon), required for normal yeast chromosomalreplication; expression peaks at the G1/S phaseboundary; potential Cdc28p substrate 0.001120.05428 S000005181YTP1YNL237WverifiedProbable type-III integral membrane protein of unknownfunction, has regions of similarity tomitochondrial electron transport proteins 0.001120.05428&radic S000004886ZDS1YMR273CverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; has arole in localization of Bcy1p, a regulatorysubunit of protein kinase A; implicated in mRNAnuclear export 0.001110.05379 S000004272YLR282CDubiousHypothetical protein 0.00110.05326&radic S000002834YDR426CDubiousHypothetical protein 0.00110.05326&radic&radic S000003145YGL177WDubiousHypothetical protein 0.00110.05326&radic S000000161YBL065WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene 0.00110.05326&radic S000003156YGL188CDubiousHypothetical protein 0.00110.05326&radic&radic S000003622YJL086CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 0.00110.05326&radic&radic S000003100YGL132WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C 0.00110.05326&radic&radic S000006257YPR053CDubiousHypothetical protein 0.00110.05326&radic S000004368PSY3YLR376CuncharacterizedProtein of unknown function; deletion results in a mutatorphenotype suggesting a role for this protein asa mutational suppressor; deletion increasessensitivity to anticancer drugs oxaliplatin andcisplatin but not mitomycin C 0.00110.05299&radic&radic&radic&radic S000005194RAD50YNL250WverifiedSubunit of MRX complex, with Mre11p and Xrs2p, involved inprocessing double-strand DNA breaks invegetative cells, initiation of meiotic DSBs,telomere maintenance, and nonhomologous endjoining 0.001090.05299&radic S000003804POL32YJR043CverifiedThird subunit of DNA polymerase delta, involved in chromosomalDNA replication; required for error-prone DNAsynthesis in the presence of DNA damage andprocessivity; interacts with Hys2p, PCNA(Pol30p), and Pol1p 0.00110.05299 S000005551HST3YOR025WverifiedMember of the Sir2 family of NAD(+)-dependent proteindeacetylases; involved along with Hst4p intelomeric silencing, cell cycle progression,radiation resistance, genomic stability andshort-chain fatty acid metabolism 0.001090.05277&radic&radic S000002752YDR344CDubiousHypothetical protein 0.001090.05245&radic S000001029YHL037CDubiousHypothetical protein 0.001090.05245&radic&radic S000004165CBF5YLR175WverifiedPseudouridine synthase catalytic subunit of box H/ACA smallnucleolar ribonucleoprotein particles(snoRNPs), acts on both large and small rRNAsand on snRNA U2 0.001090.05245&radic&radic S000003497YGR265WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase 0.001090.05245&radic S000002987CKB1YGL019WverifiedBeta regulatory subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA1, CKA2 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 0.001080.05211&radic&radic S000002771ESC2YDR363WverifiedProtein involved in mating-type locus silencing, interacts withSir2p; probably functions to recruit orstabilize Sir proteins 0.001070.05162 S000003141KEM1YGL173CverifiedEvolutionarily-conserved 5'-3' exonuclease component ofcytoplasmic processing (P) bodies involved inmRNA decay; plays a role inmicrotubule-mediated processes, filamentousgrowth, ribosomal RNA maturation, and telomeremaintenance 0.001070.05053 S000005201SIP3YNL257CverifiedProtein that activates transcription through interaction withDNA-bound Snf1p, C-terminal region has aputative leucine zipper motif; potential Cdc28psubstrate 0.001060.05053&radic&radic S000004365VID22YLR373CverifiedGlycosylated integral membrane protein localized to the plasmamembrane; plays a role infructose-1,6-bisphosphatase (FBPase)degradation; involved in FBPase transport fromthe cytosol to Vid (vacuole import anddegradation) vesicles 0.001060.05053 S000000019CCR4YAL021CverifiedComponent of the CCR4-NOT transcriptional complex, which isinvolved in regulation of gene expression;component of the major cytoplasmic deadenylase,which is involved in mRNA poly(A) tailshortening 0.001050.05041&radic S000003143SAE2YGL175CverifiedProtein with a role in accurate meiotic and mitoticdouble-strand break repair; phosphorylated inresponse to DNA damage and required for normalresistance to DNA-damaging agents 0.001050.05019&radic&radic&radic S000002260CDC2YDL102WverifiedCatalytic subunit of DNA polymerase delta; required forchromosomal DNA replication during mitosis andmeiosis, intragenic recombination, repair ofdouble strand DNA breaks, and DNA replicationduring nucleotide excision repair (NER) 0.001050.05019 S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.001040.04973&radic S000000120NCL1YBL024WverifiedS-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase,methylates cytosine to m5C at several positionsin tRNAs and intron-containing pre-tRNAs;similar to Nop2p and human proliferationassociated nucleolar protein p120 0.001040.04972&radic S000002490RRP8YDR083WverifiedNucleolar protein involved in rRNA processing, pre-rRNAcleavage at site A2; also involved in telomeremaintenance; mutation is synthetically lethalwith a gar1 mutation 0.001030.04923&radic&radic S000002897SLD5YDR489WverifiedSubunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), whichis localized to DNA replication origins andimplicated in assembly of the DNA replicationmachinery 0.001040.04923 S000002360TRM8YDL201WverifiedSubunit of a tRNA methyltransferase complex composed of Trm8pand Trm82p that catalyzes 7-methylguanosinemodification of tRNA 0.001030.04873 S000000818BIM1YER016WverifiedMicrotubule-binding protein that together with Kar9p makes upthe cortical microtubule capture site anddelays the exit from mitosis when the spindleis oriented abnormally 0.001020.04869 S000005143GCR2YNL199CverifiedTranscriptional activator of genes involved in glycolysis;interacts and functions with the DNA-bindingprotein Gcr1p 0.001020.04843 S000003335NOP7YGR103WverifiedNucleolar protein involved in rRNA processing and 60S ribosomalsubunit biogenesis; constituent of severaldifferent pre-ribosomal particles; required forexit from G0 and the initiation ofcell proliferation 0.000990.045&radic S000000133APL3YBL037WverifiedAlpha-adaptin, large subunit of the clathrin associated proteincomplex (AP-2); involved in vesicle mediatedtransport 0.000980.04488&radic S000000673PAT1YCR077CverifiedTopoisomerase II-associated deadenylation-dependentmRNA-decapping factor; also required forfaithful chromosome transmission, maintenanceof rDNA locus stability, and protection of mRNA3'-UTRs from trimming; functionally linked toPab1p 0.000960.04418&radic S000005187SLA2YNL243WverifiedTransmembrane actin-binding protein involved in membranecytoskeleton assembly and cell polarization;adaptor protein that links actin to clathrinand endocytosis; present in the actin corticalpatch of the emerging bud tip; dimer in vivo 0.000970.04418 S000005667ARP8YOR141CverifiedNuclear actin-related protein involved in chromatin remodeling,component of chromatin-remodeling enzymecomplexes 0.000960.04383 S000001234CTF8YHR191CverifiedSubunit of a complex with Ctf18p that shares some subunits withReplication Factor C and is required for sisterchromatid cohesion 0.000950.04318&radic S000004683CTF18YMR078CverifiedSubunit of a complex with Ctf8p that shares some subunits withReplication Factor C and is required for sisterchromatid cohesion; may have overlappingfunctions with Rad24p in the DNA damagereplication checkpoint 0.000940.04266 S000004681PDS5YMR076CverifiedProtein required for establishment and maintenance of sisterchromatid condensation and cohesion,colocalizes with cohesin on chromosomes in aninterdependent manner, may function as aprotein-protein interaction scaffold 0.000930.04224 S000004527MFT1YML062CverifiedSubunit of the THO complex, which is a nuclear complexcomprised of Hpr1p, Mft1p, Rlr1p, and Thp2p,that is involved in transcription elongationand mitotic recombination; involved in telomeremaintenance 0.000920.04181 S000000787CIN8YEL061CverifiedKinesin motor protein involved in mitotic spindle assembly andchromosome segregation 0.000920.04156 S000004490TSA1YML028WverifiedUbiquitous housekeeping thioredoxin peroxidase, reducesreactive oxygen, nitrogen and sulfur speciesusing thioredoxin as hydrogen donor; mediatesredox regulation of the nuclear localization ofYap1p; deletion results in mutator phenotype 0.000910.04156 S000004534POB3YML069WverifiedSubunit of the heterodimeric FACT complex (Spt16p-Pob3p), whichfacilitates RNA Polymerase II transcriptionelongation through nucleosomes by destabilizingand then reassembling nucleosome structure 0.000910.04127 S000000298PBY1YBR094WuncharacterizedP-body associated protein 0.000910.04127&radic&radic&radic S000002841YDR433WDubiousHypothetical protein 0.000890.04006&radic&radic S000005372HTZ1YOL012CverifiedHistone variant H2AZ, exchanged for histone H2A in nucleosomesby the SWR1 complex; involved intranscriptional regulation through preventionof the spread of silent heterochromatin 0.000880.03996 S000001780SIS2YKR072CverifiedNegative regulatory subunit of the protein phosphatase 1 Ppz1p;involved in ion homeostasis and cell cycleprogression 0.000870.03979&radic&radic S000000897RAD51YER095WverifiedStrand exchange protein, forms a helical filament with DNA thatsearches for homology; involved in therecombinational repair of double-strand breaksin DNA during vegetative growth and meiosis;homolog of Dmc1p and bacterial RecA protein 0.000860.03951&radic S000006147NEW1YPL226WverifiedATP binding cassette family member; Asn/Gln-rich rich regionsupports [NU+] prion formation, susceptibilityto [PSI+] prion induction and aggregation of afragment of the human Machado-Joseph Diseaseprotein 0.000860.03951 S000001160ORC6YHR118CverifiedSubunit of the origin recognition complex, which directs DNAreplication by binding to replication originsand is also involved in transcriptionalsilencing; may be phosphorylated by Cdc28p 0.000850.03895 S000003175SPT16YGL207WverifiedSubunit of the heterodimeric FACT complex (Spt16p-Pob3p),facilitates RNA Polymerase II transcriptionelongation through nucleosomes by destabilizingand then reassembling nucleosome structure 0.000850.03895 S000000253REB1YBR049CverifiedRNA polymerase I enhancer binding protein; DNA binding proteinwhich binds to genes transcribed by both RNApolymerase I and RNA polymerase II; requiredfor termination of RNA polymerase Itranscription 0.000840.0381 S000003818CDC8YJR057WverifiedThymidylate and uridylate kinase, functions in de novobiosynthesis of pyrimidinedeoxyribonucleotides; converts dTMP to dTDP anddUMP to dUTP; essential for mitotic and meioticDNA replication; homologous to S. pombe Tmp1p 0.000830.0381&radic S000001494CCE1YKL011CverifiedMitochondrial cruciform cutting endonuclease, cleaves Hollidayjunctions formed during recombination ofmitochondrial DNA 0.000820.03767&radic&radic S000001790NUP133YKR082WverifiedSubunit of the Nup84p subcomplex of the nuclear pore complex(NPC), localizes to both sides of the NPC,required to establish a normalnucleocytoplasmic concentration gradient of theGTPase Gsp1p 0.000820.03767&radic S000000662RAD18YCR066WverifiedProtein involved in postreplication repair; bindssingle-stranded DNA and has single-stranded DNAdependent ATPase activity; forms heterodimerwith Rad6p; contains RING-finger motif 0.000820.03767 S000002687RNH202YDR279WverifiedRibonuclease H2 subunit, required for RNase H2 activity 0.000820.03719 S000003054MAD1YGL086WverifiedCoiled-coil protein involved in the spindle-assemblycheckpoint; phosphorylated by Mps1p uponcheckpoint activation which leads to inhibitionof the activity of the anaphase promotingcomplex; forms a complex with Mad2p 0.000820.03719 S000000098HTB2YBL002WverifiedOne of two nearly identical (see HTB1) histone H2B subtypesrequired for chromatin assembly and chromosomefunction; Rad6p-Bre1p-Lge1p mediatedubiquitination regulates transcriptionalactivation, meiotic DSB formation and H3methylation 0.000790.03639 S000002161MCD1YDL003WverifiedEssential protein required for sister chromatid cohesion inmitosis and meiosis; subunit of the cohesincomplex; expression is cell cycle regulated andpeaks in S phase 0.000790.03639 S000000572HMLALPHA2YCL067CverifiedSilenced copy of ALPHA2 at HML; homeobox-domain protein thatassociates with Mcm1p in haploid cells torepress a-specific gene expression andinteracts with a1p in diploid cells to represshaploid-specific gene expression 0.000790.03639 S000006339CTF4YPR135WverifiedChromatin-associated protein, required for sister chromatidcohesion; interacts with DNA polymerase alpha(Pol1p) and may link DNA synthesis to sisterchromatid cohesion 0.000790.03639&radic S000004375SMC6YLR383WverifiedProtein involved in structural maintenance of chromosomes;essential subunit of Mms21-Smc5-Smc6 complex;required for growth, DNA repair,interchromosomal and sister chromatidrecombination; homologous to S. pombe rad18 0.000780.03577 S000002767VID21YDR359CverifiedComponent of the NuA4 histone acetyltransferase complex 0.000780.03577&radic S000000191YBL095WUncharacterizedHypothetical protein 0.000790.03577&radic&radic S000000264ORC2YBR060CverifiedSubunit of the origin recognition complex, which directs DNAreplication by binding to replication originsand is also involved in transcriptionalsilencing; may be phosphorylated by Cdc28p 0.000780.03577 S000000449ISW1YBR245CverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPase thatforms a complex with Ioc2p and Ioc4p toregulate transcription elongation, and acomplex with Ioc3p to repress transcriptioninitiation 0.000760.03536 S000004984BDP1YNL039WverifiedEssential subunit of RNA polymerase III transcription factor(TFIIIB), which is involved in transcription ofgenes encoding tRNAs, 5S rRNA, U6 snRNA, andother small RNAs 0.000750.03483 S000000213HHF1YBR009CverifiedOne of two identical histone H4 proteins (see also HHF2); corehistone required for chromatin assembly andchromosome function; contributes to telomericsilencing; N-terminal domain involved inmaintaining genomic integrity 0.000750.03483 S000004329VRP1YLR337CverifiedProline-rich actin-associated protein involved in cytoskeletalorganization and cytokinesis; related tomammalian Wiskott-Aldrich syndrome protein(WASP)-interacting protein (WIP) 0.000750.03477 S000002160NHP10YDL002CverifiedProtein related to mammalian high mobility group proteins;likely component of the INO80 complex, which isan ATP-dependent chromatin-remodeling complex 0.000740.03431 S000003288RSC1YGR056WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; containstwo essential bromodomains, a bromo-adjacenthomology (BAH) domain, and an AT hook 0.000730.03417 S000002545HPR1YDR138WverifiedSubunit of THO/TREX complexes that couple transcriptionelongation with mitotic recombination and withmRNA metabolism and export, subunit of an RNAPol II complex; regulates lifespan; involved intelomere maintenance; similar to Top1p 0.000730.03417 S000002723IPK1YDR315CverifiedInositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear proteinrequired for synthesis of1,2,3,4,5,6-hexakisphosphate (phytate), whichis integral to cell function; has 2 motifsconserved in other fungi; ipk1 gle1 doublemutant is inviable 0.000730.03417 S000000340MEC1YBR136WverifiedGenome integrity checkpoint protein and PI kinase superfamilymember; signal transducer required for cellcycle arrest and transcriptional responsesprompted by damaged or unreplicated DNA;monitors and participates in meioticrecombination 0.000730.03409 S000006305SNT309YPR101WverifiedComponent of NineTeen complex (NTC) containing Prp19p involvedin mRNA splicing, interacts physically andgenetically with Prp19p 0.000730.03347 S000003626DPB11YJL090CverifiedEssential BRCT repeat protein, required on the prereplicativecomplex at replication origins for loading DNApolymerases to initiate DNA synthesis, alsorequired for S/M checkpoint control 0.000720.03347 S000005504NOP8YOL144WverifiedNucleolar protein required for 60S ribosomal subunit biogenesis 0.000730.03347&radic S000000968DNF1YER166WverifiedAminophospholipid translocase (flippase) that localizesprimarily to the plasma membrane; contributesto endocytosis, protein transport and cellpolarity; type 4 P-type ATPase 0.000730.03347 S000000099HTA2YBL003CverifiedOne of two nearly identical (see also HTA1) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.000720.03347 S000001239UTP9YHR196WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000720.03347 S000005334BRE5YNR051CverifiedUbiquitin protease cofactor, forms deubiquitination complexwith Ubp3p that coregulates anterograde andretrograde transport between the endoplasmicreticulum and Golgi compartments; null issensitive to brefeldin A 0.000720.03347 S000004334FKS1YLR342WverifiedCatalytic subunit of 1,3-beta-D-glucan synthase, functionallyredundant with alternate catalytic subunitGsc2p; binds to regulatory subunit Rho1p;involved in cell wall synthesis andmaintenance; localizes to sites of cell wallremodeling 0.000710.03329 S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.000710.03329&radic S000004811CIK1YMR198WverifiedKinesin-associated protein required for both karyogamy andmitotic spindle organization, interacts stablyand specifically with Kar3p and may function totarget this kinesin to a specific cellularrole; has similarity to Vik1p 0.000710.03329 S000002732UTP4YDR324CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000710.03329 S000001297CKA1YIL035CverifiedAlpha catalytic subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA2, CKB1 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 0.000710.03323&radic S000005559EXO1YOR033Cverified5'-3' exonuclease and flap-endonuclease involved inrecombination, double-strand break repair andDNA mismatch repair; member of the Rad2pnuclease family, with conserved N and Inuclease domains 0.000710.03291 S000004839TMA29YMR226CverifiedNADP(+)-dependent dehydrogenase; acts on serine,L-allo-threonine, and other 3-hydroxy acids;green fluorescent protein fusion proteinlocalizes to the cytoplasm and nucleus; mayinteract with ribosomes, based onco-purification experiments 0.00070.03258 S000003512PXR1YGR280CverifiedEssential protein involved in rRNA and snoRNA maturation;competes with TLC1 RNA for binding to Est2p,suggesting a role in regulation of telomerase;human homolog inhibits telomerase; contains aG-patch RNA interacting domain 0.00070.03258 S000001504MAK11YKL021CverifiedProtein essential for cell growth and replication of M dsRNAvirus; contains four beta-transducin repeats 0.00070.03258&radic&radic S000000322TEF2YBR118WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF1; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.00070.03258 S000003730CDC6YJL194WverifiedEssential ATP-binding protein required for DNA replication,component of the pre-replicative complex(pre-RC) which requires ORC to associate withchromatin and is in turn required for Mcm2-7pDNA association; homologous to S. pombe Cdc18p 0.00070.03258 S000002467MAK21YDR060WverifiedConstituent of 66S pre-ribosomal particles, required for large(60S) ribosomal subunit biogenesis; involved innuclear export of pre-ribosomes; required formaintenance of dsRNA virus; homolog of humanCAATT-binding protein 0.00070.03258 S000002720SSF2YDR312WverifiedProtein required for ribosomal large subunit maturation,functionally redundant with Ssf1p; member ofthe Brix family 0.000690.03226 S000001927SMC2YFR031CverifiedComponent of the condensin complex, essential SMC chromosomalATPase family member that forms a complex withSmc4p to form the active ATPase; Smc2p/Smc4pcomplex binds DNA, possibly in the cleft formedby the coiled-coil of the folded dimer 0.000690.03226 S000000758MCM3YEL032WverifiedProtein involved in DNA replication; component of the Mcm2-7hexameric complex that binds chromatin as apart of the pre-replicative complex 0.000690.03226 S000000914LSM4YER112WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.00070.03226 S000004925PSE1YMR308CverifiedKaryopherin/importin that interacts with the nuclear porecomplex; acts as the nuclear import receptorfor specific proteins, including Pdr1p, Yap1p,Ste12p, and Aft1p 0.00070.03226 S000003934SOF1YLL011WverifiedEssential subunit of the U3 (box C+D) snRNP complex requiredfor 2' O-methylation of pre-RNA; has similarityto the beta subunit of trimeric G-proteins andthe splicing factor Prp4p 0.000690.03221 S000005571TOM6YOR045WverifiedComponent of the TOM (translocase of outer membrane) complexresponsible for recognition and initial importsteps for all mitochondrially directedproteins; promotes assembly and stability ofthe TOM complex 0.000690.03221 S000000103SLA1YBL007CverifiedCytoskeletal protein binding protein required for assembly ofthe cortical actin cytoskeleton; contains 3 SH3domains; interacts with proteins regulatingactin dynamics and with proteins required forendocytosis 0.000680.03214 S000004885SCS7YMR272CverifiedSphingolipid alpha-hydroxylase, functions in thealpha-hydroxylation of sphingolipid-associatedvery long chain fatty acids, has bothcytochrome b5-like and hydroxylase/desaturasedomains, not essential for growth 0.000690.03214&radic&radic S000001768UTP30YKR060WuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.000690.03214&radic S000002433YDR026CUncharacterizedPutative transcription factor; Myb-like DNA-binding protein that may bind to the Ter region of rDNA; interacts physically with Fob1p, although this interaction is not involved in replication fork arrest 0.000680.03188 S000005169CNM67YNL225CverifiedComponent of the spindle pole body outer plaque; required forspindle orientation and mitotic nuclearmigration 0.000680.03188 S000000455MRPS5YBR251WverifiedMitochondrial ribosomal protein of the small subunit 0.000680.03188 S000006314RPC40YPR110CverifiedRNA polymerase subunit, common to RNA polymerase I and III 0.000680.03181 S000003063VPS45YGL095CverifiedProtein of the Sec1p/Munc-18 family, essential for vacuolarprotein sorting; required for the function ofPep12p and the early endosome/late Golgi SNARETlg2p; essential for fusion of Golgi-derivedvesicles with the prevacuolar compartment 0.000680.03181 S000002411RAD57YDR004WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad55p 0.000680.03181&radic S000001505CDC16YKL022CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; required forsporulation 0.000680.03181 S000005587CKA2YOR061WverifiedAlpha' catalytic subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA1, CKB1 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 0.000670.03156 S000004447YLR455WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR455W is not an essential gene 0.000670.03156 S000004765TPP1YMR156CverifiedDNA 3'-phosphatase that functions in repair of endogenousdamage of double-stranded DNA, activity isspecific for removal of 3' phosphates at strandbreaks; has similarity to the l-2-haloaciddehalogenase superfamily 0.000670.03156 S000004349RSC2YLR357WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; involvedin telomere maintenance 0.000670.03156 S000001924CDC14YFR028CverifiedProtein phosphatase required for mitotic exit; located in thenucleolus until liberated by the FEAR andMitotic Exit Network in anaphase, enabling itto act on key substrates to effect a decreasein CDK/B-cyclin activity and mitotic exit 0.000670.03156&radic S000002733YCG1YDR325WverifiedNon-SMC subunit of the condensin complex(Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required forestablishment and maintenance of chromosomecondensation, chromosome segregation and forchromatin binding of the condensin complex 0.000670.03156 S000000913SWI4YER111CverifiedDNA binding component of the SBF complex (Swi4p-Swi6p), atranscriptional activator that in concert withMBF (Mbp1-Swi6p) regulates late G1-specifictranscription of targets including cyclins andgenes required for DNA synthesis and repair 0.000660.03142 S000000377UMP1YBR173CverifiedShort-lived chaperone required for correct maturation of the20S proteasome; degraded by proteasome uponcompletion of its assembly; involved inubiquitin-mediated proteolysis; mutantdefective in degradation of short-livedproteins 0.000660.03128 S000005735NPT1YOR209CverifiedNicotinate phosphoribosyltransferase, acts in the salvagepathway of NAD+ biosynthesis; required forsilencing at rDNA and telomeres and has a rolein silencing at mating-type loci; localized tothe nucleus 0.000660.03128 S000006220TIF6YPR016CverifiedConstituent of 66S pre-ribosomal particles, has similarity tohuman translation initiation factor 6 (eIF6);may be involved in the biogenesis and orstability of 60S ribosomal subunits 0.000660.03128&radic S000001212DBP8YHR169WverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in biogenesis of the 40S ribosomalsubunit 0.000660.03124 S000006341RRP9YPR137WverifiedProtein involved in pre-rRNA processing, associated with U3snRNP; component of small ribosomal subunit(SSU) processosome; ortholog of the humanU3-55k protein 0.000660.03124 S000006115DDC1YPL194WverifiedDNA damage checkpoint protein, part of a PCNA-like complexrequired for DNA damage response, required forpachytene checkpoint to inhibit cell cycle inresponse to unrepaired recombinationintermediates; potential Cdc28p substrate 0.000660.03121&radic S000001551NUP100YKL068WverifiedSubunit of the nuclear pore complex (NPC) that is localized toboth sides of the pore; contains a repetitiveGLFG motif that interacts with mRNA exportfactor Mex67p and with karyopherin Kap95p;homologous to Nup116p 0.000660.03109 S000002806UTP5YDR398WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000660.03109 S000000110RRN6YBL014CverifiedProtein involved in the transcription of 35S rRNA genes by RNApolymerase I; component of the core factor (CF)complex also composed of Rrn11p, Rrn7p andTATA-binding protein 0.000650.03098 S000004597MSC1YML128CverifiedProtein of unknown function; mutant is defective in directingmeiotic recombination events to homologouschromatids; the authentic, non-tagged proteinis detected in highly purified mitochondria inhigh-throughput studies 0.000650.03098 S000004728RPL15BYMR121CverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl15Ap and has similarity to ratL15 ribosomal protein; binds to 5.8 S rRNA 0.000650.03098&radic&radic&radic S000002773ESF1YDR365CverifiedNucleolar protein involved in pre-rRNA processing; depletioncauses severely decreased 18S rRNA levels 0.000650.03098 S000004327NUP2YLR335WverifiedProtein involved in nucleocytoplasmic transport, binds toeither the nucleoplasmic or cytoplasmic facesof the nuclear pore complex depending onRan-GTP levels; also has a role in chromatinorganization 0.000650.03098 S000000653PWP2YCR057CverifiedConserved 90S pre-ribosomal component essential for properendonucleolytic cleavage of the 35 S rRNAprecursor at A0, A1, and A2 sites; containseight WD-repeats; PWP2 deletion leads todefects in cell cycle and bud morphogenesis 0.000650.03098 S000004967YNL022CUncharacterizedPutative protein of unknown function with seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to a single spot in the nucleus; YNL022C is not an essential gene 0.000650.03098&radic S000004415ATG17YLR423CverifiedProtein that interacts with and is required for activation ofApg1p protein kinase; involved in autophagy butnot in the Cvt (cytoplasm to vacuole targeting)pathway 0.000650.03097 S000006029YPL108WUncharacterizedHypothetical protein 0.000650.03097 S000001073RRM3YHR031CverifiedDNA helicase involved in rDNA replication and Ty1transposition; relieves replication fork pausesat telomeric regions; structurally andfunctionally related to Pif1p 0.000650.03086 S000006048HHO1YPL127CverifiedHistone H1, a linker histone required for nucleosome packagingat restricted sites; suppresses DNA repairinvolving homologous recombination; notrequired for telomeric silencing, basaltranscriptional repression, or efficientsporulation 0.000650.03086 S000004852RNT1YMR239CverifiedRNAase III; cleaves a stem-loop structure at the 3' end of U2snRNA to ensure formation of the correct U2 3'end 0.000650.03086 S000000022LTE1YAL024CverifiedPutative GDP/GTP exchange factor required for mitotic exit atlow temperatures; acts as a guanine nucleotideexchange factor (GEF) for Tem1p, which is a keyregulator of mitotic exit; physicallyassociates with Ras2p-GTP 0.000650.03086 S000006005BRO1YPL084WverifiedCytoplasmic class E vacuolar protein sorting (VPS) factor thatcoordinates deubiquitination in themultivesicular body (MVB) pathway by recruitingDoa4p to endosomes 0.000650.03083 S000003327RRP46YGR095CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.000650.03083 S000002178RPN4YDL020CverifiedTranscription factor that stimulates expression of proteasomegenes; Rpn4p levels are in turn regulated bythe 26S proteasome in a negative feedbackcontrol mechanism; RPN4 is transcriptionallyregulated by various stress responses 0.000650.03083 S000004860FAA4YMR246WverifiedLong chain fatty acyl-CoA synthetase, regulates proteinmodification during growth in the presence ofethanol, functions to incorporate palmitic acidinto phospholipids and neutral lipids 0.000650.03074 S000004363ROM2YLR371WverifiedGDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutationsare synthetically lethal with mutations inrom1, which also encodes a GEP 0.000650.03074 S000001497URB1YKL014CverifiedNucleolar protein required for the normal accumulation of 25Sand 5.8S rRNAs, associated with the 27SA2pre-ribosomal particle; proposed to be involvedin the biogenesis of the 60S ribosomal subunit 0.000640.03066 S000004409VPS36YLR417WverifiedComponent of the ESCRT-II complex; contains the GLUE (GRAM LikeUbiquitin binding in EAP45) domain which isinvolved in interactions with ESCRT-I andubiquitin-dependent sorting of proteins intothe endosome 0.000640.03066 S000002537FIN1YDR130CverifiedSpindle pole body-related intermediate filament protein, formscell cycle-specific filaments between spindlepole bodies in mother and daughter cells, ableto self-assemble, expression induced duringS/G2, localization cell-cycle dependent 0.000640.03066&radic&radic S000001238NVJ1YHR195WverifiedNuclear envelope protein that interacts with the vacuolarmembrane protein Vac8p to promote formation ofnucleus-vacuole junctions during piecemealmicroautophagy of the nucleus (PMN) 0.000640.03066 S000004641CCS1YMR038CverifiedCopper chaperone for superoxide dismutase Sod1p, involved inoxidative stress protection; Met-X-Cys-X2-Cysmotif within the N-terminal portion is involvedin insertion of copper into Sod1p underconditions of copper deprivation 0.000640.03043 S000005831ISW2YOR304WverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPasecomponent that, with Itc1p, forms a complexrequired for repression of a-specific genes,INO1, and early meiotic genes during mitoticgrowth 0.000640.03043 S000002662CHL4YDR254WverifiedOuter kinetochore protein required for chromosome stability,interacts with kinetochore proteins Ctf19p,Ctf3p, and Iml3p; exhibits a two-hybridinteraction with Mif2p; association with CENDNA requires Ctf19p 0.000640.03043 S000000084FLO1YAR050WverifiedLectin-like protein involved in flocculation, cell wall proteinthat binds to mannose chains on the surface ofother cells, confers floc-forming ability thatis chymotrypsin sensitive and heat resistant;similar to Flo5p 0.000640.03043&radic&radic S000001492MRT4YKL009WverifiedProtein involved in mRNA turnover and ribosome assembly,localizes to the nucleolus 0.000640.03043 S000002968LSB3YFR024C-AverifiedProtein containing a C-terminal SH3 domain; binds Las17p, whichis a homolog of human Wiskott-Aldrich Syndromeprotein involved in actin patch assembly andactin polymerization 0.000640.03043 S000003502YTA7YGR270WverifiedProtein of unknown function, member of CDC48/PAS1/SEC18 familyof ATPases, potentially phosphorylated byCdc28p 0.000640.03043 S000004362ARC18YLR370CverifiedSubunit of the ARP2/3 complex, which is required for themotility and integrity of cortical actinpatches 0.000640.03042 S000001561DHR2YKL078WverifiedPredominantly nucleolar DEAH-box RNA helicase, required for 18SrRNA synthesis 0.000640.0304 S000004882TMA23YMR269WverifiedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to thenucleolus; identified in proteomic screens ofribosomal complexes; YMR269W is not anessential gene 0.000640.0304 S000002971CDH1YGL003CverifiedCell-cycle regulated activator of the anaphase-promotingcomplex/cyclosome (APC/C), which directsubiquitination of mitotic cyclins resulting inexit from mitosis; targets the APC/C tospecific substrates including CDC20, ASE1 andCIN8 0.000640.0304 S000006132NIP7YPL211WverifiedNucleolar protein required for 60S ribosome subunit biogenesis,constituent of 66S pre-ribosomal particles;physically interacts with Nop8p and the exosomesubunit Rrp43p 0.000640.0304 S000003062PAN2YGL094CverifiedEssential subunit of the Pan2p-Pan3p poly(A)-ribonucleasecomplex, which acts to control poly(A) taillength and regulate the stoichiometry andactivity of postreplication repair complexes 0.000630.03022 S000004045SPT8YLR055CverifiedSubunit of the SAGA transcriptional regulatory complex but notpresent in SAGA-like complex SLIK/SALSA,required for SAGA-mediated inhibition at somepromoters 0.000630.03022 S000001058YSC84YHR016CverifiedProtein involved in the organization of the actin cytoskeleton;contains SH3 domain similar to Rvs167p 0.000630.03022 S000003727RPS14BYJL191WverifiedRibosomal protein 59 of the small subunit, required forribosome assembly and 20S pre-rRNA processing;mutations confer cryptopleurine resistance;nearly identical to Rps14Ap and similar to E.coli S11 and rat S14 ribosomal proteins 0.000630.03022 S000003394TIF4631YGR162WverifiedTranslation initiation factor eIF4G, subunit of the mRNAcap-binding protein complex (eIF4F) that alsocontains eIF4E (Cdc33p); associates with thepoly(A)-binding protein Pab1p, also interactswith eIF4A (Tif1p); homologous to Tif4632p 0.000630.03022 S000003723SWE1YJL187CverifiedProtein kinase that regulates the G2/M transition by inhibitionof Cdc28p kinase activity; localizes to thenucleus and to the daughter side of themother-bud neck; homolog of S. pombe Wee1p;potential Cdc28p substrate 0.000630.03022&radic&radic S000000880YER078CUncharacterizedMetallopeptidase, localized to the mitochondrial matrix 0.000630.03022 S000005483HRP1YOL123WverifiedSubunit of cleavage factor I, a five-subunit complex requiredfor the cleavage and polyadenylation ofpre-mRNA 3' ends; RRM-containing heteronuclearRNA binding protein and hnRNPA/B family memberthat binds to poly (A) signal sequences 0.000630.03022 S000001353YIL091CUncharacterizedProtein required for cell viability 0.000630.03004 S000004058FYV7YLR068WverifiedProtein of unknown function, required for survival uponexposure to K1 killer toxin; involved inprocessing the 35S rRNA primary transcript togenerate the 20S and 27SA2 pre-rRNA transcripts 0.000630.03004 S000002831CAD1YDR423CverifiedAP-1-like bZIP transcriptional activator involved in multiplestress responses, iron metabolism, andpleiotropic drug resistance; controls a set ofgenes involved in stabilizing proteins, bindsconsensus sequence TTACTAA; 5' UTR containsuORFs 0.000630.03004&radic&radic S000005783CDC31YOR257WverifiedComponent of the spindle pole body (SPB) half-bridge, requiredfor SPB duplication in mitosis and meiosis II;homolog of mammalian centrin; interacts withKar1p 0.000630.03004&radic&radic S000003515YGR283CUncharacterizedPutative protein of unknown function; deletion mutant is resistant to fluconazole; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus 0.000620.02986 S000007223LUG1YCR087C-AuncharacterizedPutative protein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to thenucleolus; YCR087C-A is not an essential gene 0.000620.02986 S000004075ARP6YLR085CverifiedActin-related protein that binds nucleosomes; a component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.000620.02986 S000000121RRN10YBL025WverifiedProtein involved in promoting high level transcription of rDNA,subunit of UAF (upstream activation factor) forRNA polymerase I 0.000620.02986&radic S000005558HMS1YOR032CverifiedBasic helix-loop-helix (bHLH) protein with similarity tomyc-family transcription factors;overexpression confers hyperfilamentous growthand suppresses the pseudohyphal filamentationdefect of a diploid mep1 mep2 homozygous nullmutant 0.000620.02986 S000002222UBC9YDL064WverifiedSUMO-conjugating enzyme involved in the Smt3p conjugationpathway; nuclear protein required for S- andM-phase cyclin degradation and mitotic control;involved in proteolysis mediated by theanaphase-promoting complex cyclosome (APCC) 0.000620.02986 S000000804NOP16YER002WverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis 0.000620.02986 S000003360UTP8YGR128CverifiedNucleolar protein required for export of tRNAs from thenucleus; also copurifies with the small subunit(SSU) processome containing the U3 snoRNA thatis involved in processing of pre-18S rRNA 0.000620.02976 S000001108SSF1YHR066WverifiedConstituent of 66S pre-ribosomal particles, required forribosomal large subunit maturation;functionally redundant with Ssf2p; member ofthe Brix family 0.000620.02976 S000005091LSM7YNL147WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.000620.02976 S000001800SRP40YKR092CverifiedNucleolar, serine-rich protein with a role in preribosomeassembly or transport; may function as achaperone of small nucleolar ribonucleoproteinparticles (snoRNPs); immunologically andstructurally to rat Nopp140 0.000620.02976 S000000124YBL028CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis 0.000620.02976 S000001486MRP17YKL003CverifiedMitochondrial ribosomal protein of the small subunit; MRP17exhibits genetic interactions with PET122,encoding a COX3-specific translationalactivator 0.000620.02976 S000000301VPS15YBR097WverifiedMyristoylated serine/threonine protein kinase involved invacuolar protein sorting; functions as amembrane-associated complex with Vps34p; activeform recruits Vps34p to the Golgi membrane;interacts with the GDP-bound form of Gpa1p 0.000620.02976 S000001358YIL096CUncharacterizedPutative protein of unknown function; associates with precursors of the 60S ribosomal subunit 0.000620.02969 S000004138PEP3YLR148WverifiedVacuolar peripheral membrane protein that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, required for vacuolarbiogenesis, forms complex with Pep5p thatmediates protein transport to the vacuole 0.000620.02969 S000005274RPD3YNL330CverifiedHistone deacetylase; regulates transcription and silencing 0.000620.02969 S000006078TGS1YPL157WverifiedTrimethyl guanosine synthase, conserved nucleolar methyltransferase responsible for conversion of them(7)G cap structure of snRNAs and snoRNAs tom(2,2,7)G; also required for ribosome synthesisand nucleolar morphology 0.000620.02969 S000002476DOA4YDR069CverifiedUbiquitin hydrolase, required for recycling ubiquitin fromproteasome-bound ubiquitinated intermediates,acts at the late endosome/prevacuolarcompartment to recover ubiquitin fromubiquitinated membrane proteins en route to thevacuole 0.000620.02969 S000005028END3YNL084CverifiedEH domain-containing protein involved in endocytosis, actincytoskeletal organization and cell wallmorphogenesis; forms a complex with Sla1p andPan1p 0.000610.02946 S000006177CLN2YPL256CverifiedG1 cyclin involved in regulation of the cell cycle; activatesCdc28p kinase to promote the G1 to S phasetransition; late G1 specific expression dependson transcription factor complexes, MBF(Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) 0.000610.02946 S000003743LAA1YJL207CverifiedProtein of unknown function, proposed to function as a largeAP-1 accessory protein; colocalizes withclathrin to the late-Golgi apparatus; YJL207Cis a non-essential gene 0.000610.02946&radic&radic S000003053YGL085WUncharacterizedPutative protein of unknown function, has homology to Staphylococcus aureus nuclease; green fluorescent protein (GFP)-fusion protein localizes to mitochondria 0.000610.02946&radic&radic S000003169MCM6YGL201CverifiedProtein involved in DNA replication; component of the Mcm2-7hexameric complex that binds chromatin as apart of the pre-replicative complex 0.000610.02946 S000005054NOP15YNL110CverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis; localizes to bothnucleolus and cytoplasm 0.000610.02946 S000000885GET2YER083CverifiedSubunit of the GET complex; required for meiotic nucleardivision and for the retrieval of HDEL proteinsfrom the Golgi to the ER in an ERD2 dependentfashion; may be involved in cell wall function 0.000610.02946&radic S000004441FPR4YLR449WverifiedPeptidyl-prolyl cis-trans isomerase (PPIase) (prolineisomerase) localized to the nucleus; catalyzesisomerization of proline residues in histonesH3 and H4, which affects lysine methylation ofthose histones 0.000610.02946 S000003797HUL4YJR036CverifiedProtein with similarity to hect domain E3 ubiquitin-proteinligases, not essential for viability 0.000610.02946 S000004252YPT6YLR262CverifiedGTPase, Ras-like GTP binding protein involved in the secretorypathway, required for fusion ofendosome-derived vesicles with the late Golgi,maturation of the vacuolar carboxypeptidase Y;has similarity to the human GTPase, Rab6 0.000610.02946 S000000521DCC1YCL016CverifiedSubunit of a complex with Ctf8p and Ctf18p that shares somecomponents with Replication Factor C, requiredfor sister chromatid cohesion and telomerelength maintenance 0.000610.02946&radic S000001886SMC1YFL008WverifiedSubunit of the multiprotein cohesin complex, essential proteininvolved in chromosome segregation and indouble-strand DNA break repair; SMC chromosomalATPase family member, binds DNA with apreference for DNA with secondary structure 0.000610.02946&radic S000005599SGO1YOR073WverifiedComponent of the spindle checkpoint, involved in sensing lackof tension on mitotic chromosomes; protectscentromeric Rec8p at meiosis I; required foraccurate chromosomal segregation at meiosis IIand for mitotic chromosome stability 0.000610.02937 S000002904PUF6YDR496CverifiedPumilio-homology domain protein that binds ASH1 mRNA at PUFconsensus sequences in the 3' UTR and repressesits translation, resulting in proper asymmetriclocalization of ASH1 mRNA 0.000610.02937 S000004738RRB1YMR131CverifiedEssential nuclear protein involved in early steps of ribosomebiogenesis; physically interacts with theribosomal protein Rpl3p 0.000610.02921 S000004280GUF1YLR289WverifiedMitochondrial GTPase of unknown function, similar to E. colielongation factor-type GTP-binding protein LepAand to LK1236.1 from Caenorhabditis elegans 0.00060.02921 S000003406CBP4YGR174CverifiedMitochondrial protein required for assembly of ubiquinolcytochrome-c reductase complex (cytochrome bc1complex); interacts with Cbp3p and function ispartially redundant with that of Cbp3p 0.00060.02921 S000001894SPB4YFL002CverifiedPutative ATP-dependent RNA helicase, nucleolar protein requiredfor synthesis of 60S ribosomal subunits at alate step in the pathway; sediments with 66Spre-ribosomes in sucrose gradients 0.000610.02921 S000003420BUB1YGR188CverifiedProtein kinase that forms a complex with Mad1p and Bub3p thatis crucial in the checkpoint mechanism requiredto prevent cell cycle progression into anaphasein the presence of spindle damage, associateswith centromere DNA via Skp1p 0.000610.02921 S000000058GDH3YAL062WverifiedNADP(+)-dependent glutamate dehydrogenase, synthesizesglutamate from ammonia and alpha-ketoglutarate;rate of alpha-ketoglutarate utilization differsfrom Gdh1p; expression regulated by nitrogenand carbon sources 0.00060.02921&radic&radic S000004603CDC5YMR001CverifiedPolo-like kinase with similarity to Xenopus Plx1 and S. pombePlo1p; found at bud neck, nucleus and SPBs; hasmultiple functions in mitosis and cytokinesisthrough phosphorylation of substrates; may be aCdc28p substrate 0.00060.02892 S000003807TAH11YJR046WverifiedDNA replication licensing factor, required for pre-replicationcomplex assembly 0.00060.02892 S000005057RPC19YNL113WverifiedRNA polymerase subunit, common to RNA polymerases I and III 0.00060.02892 S000002936HLR1YDR528WverifiedProtein involved in regulation of cell wall composition andintegrity and response to osmotic stress;overproduction suppresses a lysis sensitive PKCmutation; similar to Lre1p, which functionsantagonistically to protein kinase A 0.00060.02892&radic&radic S000005437BRX1YOL077CverifiedNucleolar protein, constituent of 66S pre-ribosomal particles;depletion leads to defects in rRNA processingand a block in the assembly of large ribosomalsubunits; possesses a sigma(70)-likeRNA-binding motif 0.00060.02892 S000000574VBA3YCL069WverifiedPermease of basic amino acids in the vacuolar membrane 0.00060.02892&radic&radic S000003121PEX14YGL153WverifiedPeroxisomal membrane protein that is a central component of theperoxisomal protein import machinery, interactswith PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomalmatrix protein signal recognition factors andmembrane receptor Pex13p 0.00060.02892 S000006394RPC82YPR190CverifiedRNA polymerase III subunit C82 0.00060.02892 S000000346MAK5YBR142WverifiedEssential nucleolar protein, putative DEAD-box RNA helicaserequired for maintenance of M1 dsRNA virus;involved in biogenesis of large (60S) ribosomalsubunits 0.00060.02892&radic S000001540NUP120YKL057CverifiedSubunit of the Nup84p subcomplex of the nuclear pore complex(NPC), required for even distribution of NPCsaround the nuclear envelope, involved inestablishment of a normal nucleocytoplasmicconcentration gradient of the GTPase Gsp1p 0.00060.02892 S000000562PRD1YCL057WverifiedZinc metalloendopeptidase, found in the cytoplasm andintermembrane space of mitochondria; withCym1p, involved in degradation of mitochondrialproteins and of presequence peptides cleavedfrom imported proteins 0.00060.02892&radic&radic S000003593IKS1YJL057CuncharacterizedPutative serine/threonine kinase; expression is induced duringmild heat stress; deletion mutants arehypersensitive to copper sulphate and resistantto sorbate; interacts with an N-terminalfragment of Sst2p 0.00060.02892&radic&radic S000007455NOP10YHR072W-AverifiedConstituent of small nucleolar ribonucleoprotein particlescontaining H/ACA-type snoRNAs, which arerequired for pseudouridylation and processingof pre-18S rRNA 0.00060.02892 S000000388YBR184WUncharacterizedPutative protein of unknown function; YBR184W is not an essential gene 0.00060.02892&radic&radic S000005819RPS10AYOR293WverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps10Bp and has similarity to ratribosomal protein S10 0.00060.02892 S000000770IES6YEL044WverifiedProtein that associates with the INO80 chromatin remodelingcomplex under low-salt conditions 0.00060.02892&radic S000004255NEJ1YLR265CverifiedProtein involved in regulation of nonhomologous end joining;repressed by MAT heterozygosity; associateswith Lif1p and regulates its cellulardistribution 0.00060.02892&radic&radic S000005287YNR004WUncharacterizedHypothetical protein 0.00060.02892 S000000458TRS20YBR254CverifiedOne of 10 subunits of the transport protein particle (TRAPP)complex of the cis-Golgi which mediates vesicledocking and fusion; mutations in the humanhomolog cause the spondyloepiphyseal dysplasiatarda (SEDL) disorder 0.000590.02883&radic&radic S000006050TAF14YPL129WverifiedSubunit (30 kDa) of TFIID, TFIIF, and SWI/SNF complexes,involved in RNA polymerase II transcriptioninitiation and in chromatin modification,contains a YEATS domain 0.000590.02883 S000003863VPS25YJR102CverifiedComponent of the ESCRT-II complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome 0.000590.02883 S000000345YBR141CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene 0.000590.02883&radic S000001756NAP1YKR048CverifiedProtein that interacts with mitotic cyclin Clb2p; required forthe regulation of microtubule dynamics duringmitosis; controls bud morphogenesis; involvedin the transport of H2A and H2B histones to thenucleus 0.000590.02875 S000005821UAF30YOR295WverifiedSubunit of UAF (upstream activation factor), which is an RNApolymerase I specific transcription stimulatoryfactor composed of Uaf30p, Rrn5p, Rrn9p,Rrn10p, histones H3 and H4; deletion decreasescellular growth rate 0.000590.02875 S000005867RPA43YOR340CverifiedRNA polymerase I subunit A43 0.000590.02875 S000000829GAL83YER027CverifiedOne of three possible beta-subunits of the Snf1 kinase complex,allows nuclear localization of the Snf1 kinasecomplex in the presence of a nonfermentablecarbon source; contains glycogen-binding domain 0.000590.02863&radic&radic S000002544RGP1YDR137WverifiedSubunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) thatcatalyzes nucleotide exchange on Ypt6p 0.000590.02863 S000004131RRN5YLR141WverifiedProtein involved in transcription of rDNA by RNA polymerase I;transcription factor, member of UAF (upstreamactivation factor) family along with Rrn9p andRrn10p 0.000590.02863 S000004779ALD3YMR169CverifiedCytoplasmic aldehyde dehydrogenase, involved in beta-alaninesynthesis; uses NAD+ as the preferred coenzyme;very similar to Ald2p; expression is induced bystress and repressed by glucose 0.000590.02863&radic&radic S000005868RPA190YOR341WverifiedRNA polymerase I subunit; largest subunit of RNA polymerase I 0.000590.02863 S000001111RRP4YHR069CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp43p and Dis3p 0.000590.02863 S000003562RRN7YJL025WverifiedProtein involved in the transcription of 35S rRNA genes by RNApolymerase I; component of the core factor (CF)complex also composed of Rrn11p, Rrn6p andTATA-binding protein 0.000590.02863 S000004040YLR050CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene 0.000590.02863&radic&radic&radic S000006241ERV2YPR037CverifiedFlavin-linked sulfhydryl oxidase localized to the endoplasmicreticulum lumen, involved in disulfide bondformation within the ER 0.000580.02841 S000002581HMO1YDR174WverifiedChromatin associated high mobility group (HMG) family memberinvolved in genome maintenance; rDNA-bindingcomponent of the Pol I transcription system;associates with a 5'-3' DNA helicase and Fpr1p,a prolyl isomerase 0.000580.02841 S000000575YCL073CUncharacterizedProtein of unconfirmed function; displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins; coding sequence 98% identical to that of YKR106W 0.000590.02841&radic&radic S000001389YIL127CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus 0.000590.02841 S000004359RPS22BYLR367WverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps22Ap and has similarity to E.coli S8 and rat S15a ribosomal proteins 0.000590.02841 S000001128NAM8YHR086WverifiedRNA binding protein, component of the U1 snRNP protein; mutantsare defective in meiotic recombination and information of viable spores, involved in theformation of DSBs through meiosis-specificsplicing of MER2 pre-mRNA 0.000580.02841&radic&radic S000002428FAL1YDR021WverifiedNucleolar protein required for maturation of 18S rRNA, memberof the eIF4A subfamily of DEAD-boxATP-dependent RNA helicases 0.000580.02841 S000004777MLH1YMR167WverifiedProtein required for mismatch repair in mitosis and meiosis,postmeiotic segregation, and spore viability;forms a complex with Pms1p and Msh2p to repairmismatched DNA; human homolog is associatedwith hereditary non-polyposis colon cancer 0.000580.02841 S000004571NUP188YML103CverifiedSubunit of the nuclear pore complex (NPC), involved in thestructural organization of the complex and ofthe nuclear envelope, also involved in nuclearenvelope permeability, interacts with Pom152pand Nic96p 0.000580.02841 S000004424IMD3YLR432WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.000580.02841 S000004144RNH203YLR154CverifiedRibonuclease H2 subunit, required for RNase H2 activity 0.000580.02841 S000000490APE3YBR286WverifiedVacuolar aminopeptidase Y, processed to mature form by Prb1p 0.000590.02841&radic&radic&radic S000005043OCA1YNL099CverifiedPutative protein tyrosine phosphatase, required for cell cyclearrest in response to oxidative damage of DNA 0.000590.02841 S000000781POL5YEL055CverifiedProtein with sequence similarity to the human MybBP1A and weaksequence similar to B-type DNA polymerases, notrequired for chromosomal DNA replication;required for the synthesis of rRNA 0.000590.02841 S000004181PEX13YLR191WverifiedIntegral peroxisomal membrane receptor for the PTS1 peroxisomalmatrix protein signal recognition factor Pex5p,required for the translocation of peroxisomalmatrix proteins, also interacts with Pex7p andPex14p, contains a C-terminal SH3 domain 0.000590.02841 S000003547NOP9YJL010CuncharacterizedEssential nucleolar protein required for 18S rRNA synthesis 0.000590.02841 S000005204YNL260CUncharacterizedPutative protein of unknown function with similarity to a human protein overexpressed in oral cancers; localizes to the nucleus and cytoplasm; YNL260C is an essential gene 0.000580.02841&radic&radic S000006250MCM16YPR046WverifiedProtein involved in kinetochore-microtubule mediated chromosomesegregation; binds to centromere DNA 0.000580.02841 S000004699UTP15YMR093WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000590.02841 S000001312PCL7YIL050WverifiedPho85p cyclin of the Pho80p subfamily, forms a functionalkinase complex with Pho85p which phosphorylatesMmr1p and is regulated by Pho81p; involved inglycogen metabolism, expression is cell-cycleregulated 0.000580.02841&radic&radic S000000100UTP20YBL004WverifiedComponent of the small-subunit (SSU) processome, which isinvolved in the biogenesis of the 18S rRNA 0.000590.02841 S000000459YBR255WUncharacterizedProtein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 0.000580.02725&radic&radic S000001469DCG1YIR030CverifiedProtein of unknown function, expression is sensitive tonitrogen catabolite repression and regulated byDal80p; contains transmembrane domain 0.000580.02725&radic&radic S000006255MAK3YPR051WverifiedCatalytic subunit of N-terminal acetyltransferase of the NatCtype; required for replication of dsRNA virus 0.000580.02725 S000004969YNL024CUncharacterizedPutative protein of unknown function with seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL024C is not an essential gene 0.000580.02725 S000002372NOP6YDL213CuncharacterizedProtein with similarity to hydrophilins, which are involved inthe adaptive response to hyperosmoticconditions; computational analysis oflarge-scale protein-protein interaction datasuggests a possible role in rRNA processing 0.000570.02725&radic S000003466YHB1YGR234WverifiedNitric oxide oxidoreductase, flavohemoglobin involved in nitricoxide detoxification; plays a role in theoxidative and nitrosative stress responses 0.000580.02725 S000000514STP22YCL008CverifiedComponent of the ESCRT-I complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; homologous to the mouse and humanTsg101 tumor susceptibility gene; mutantsexhibit a Class E Vps phenotype 0.000580.02725 S000005507PEX11YOL147CverifiedPeroxisomal membrane protein required for peroxisomeproliferation and medium-chain fatty acidoxidation, most abundant protein in theperoxisomal membrane, regulated by Adr1p andPip2p-Oaf1p, promoter contains ORE andUAS1-like elements 0.000580.02725&radic&radic S000004710YPK2YMR104CverifiedProtein kinase with similarityto serine/threonine proteinkinase Ypk1p; functionally redundant with YPK1at the genetic level; participates in asignaling pathway required for optimal cellwall integrity; homolog of mammalian kinase SGK 0.000580.02725&radic&radic S000001340THS1YIL078WverifiedThreonyl-tRNA synthetase, essential cytoplasmic protein 0.000580.02725&radic&radic S000004476TRM9YML014WverifiedtRNA methyltransferase, catalyzes the esterification ofmodified uridine nucleotides in tRNAs, creating5-methylcarbonylmethyluridine in tRNA(Arg3) and5-methylcarbonylmethyl-2-thiouridine intRNA(Glu); may have a role in stress response 0.000580.02725 S000003726RPS22AYJL190CverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps22Bp and has similarity to E.coli S8 and rat S15a ribosomal proteins 0.000580.02725 S000004735ECM16YMR128WverifiedEssential DEAH-box ATP-dependent RNA helicase specific to theU3 snoRNP, predominantly nucleolar indistribution, required for 18S rRNA synthesis 0.000580.02725 S000000154SHP1YBL058WverifiedUBX (ubiquitin regulatory X) domain-containing protein thatregulates Glc7p phosphatase activity andinteracts with Cdc48p; interacts withubiquitylated proteins in vivo and is requiredfor degradation of a ubiquitylated modelsubstrate 0.000580.02725&radic S000002473YDR066CUncharacterizedHypothetical protein 0.000580.02725&radic&radic S000001655EBP2YKL172WverifiedEssential protein required for the maturation of 25S rRNA and60S ribosomal subunit assembly, localizes tothe nucleolus; constituent of 66S pre-ribosomalparticles 0.000580.02725&radic&radic S000003905MGM101YJR144WverifiedProtein involved in mitochondrial genome maintenance; componentof the mitochondrial nucleoid, required for therepair of oxidative mtDNA damage 0.000580.02725 S000002472YDR065WUncharacterizedProtein of unknown function, required for vacuolar acidification 0.000580.02725&radic&radic S000003722MNN5YJL186WverifiedAlpha-1,2-mannosyltransferase, responsible for addition of thesecond alpha-1,2-linked mannose of the brancheson the mannan backbone of oligosaccharides,localizes to an early Golgi compartment 0.000580.02725&radic&radic&radic S000005321DBP6YNR038WverifiedEssential protein involved in ribosome biogenesis; putativeATP-dependent RNA helicase of the DEAD-boxprotein family 0.000580.02725 S000005051YAF9YNL107WverifiedSubunit of both the NuA4 histone H4 acetyltransferase complexand the SWR1 complex, may function toantagonize silencing near telomeres; interactsdirectly with Swc4p, has homology to humanleukemogenic protein AF9, contains a YEATSdomain 0.000580.02725 S000000117HAP3YBL021CverifiedSubunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5pCCAAT-binding complex, a transcriptionalactivator and global regulator of respiratorygene expression; contains sequencescontributing to both complex assembly and DNAbinding 0.000580.02725&radic&radic S000004520IMD4YML056CverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.000580.02725 S000005649LEO1YOR123CverifiedComponent of the Paf1 complex, which associates with RNApolymerase II and is involved in histonemethylation 0.000580.02725&radic&radic S000003074MLC1YGL106WverifiedEssential light chain for myosin Myo2p; may stabilize Myo2p bybinding to the neck region; may interact withMyo1p, Iqg1p, and Myo2p to coordinate formationand contraction of the actomyosin ring withtargeted membrane deposition 0.000580.02725 S000004873TIF11YMR260CverifiedTranslation initiation factor eIF1A, essential protein thatforms a complex with Sui1p (eIF1) and the 40Sribosomal subunit and scans for the startcodon; C-terminus associates with Fun12p(eIF5B); N terminus interacts with eIF2 andeIF3 0.000570.02724&radic S000005536TIR2YOR010CverifiedPutative cell wall mannoprotein of the Srp1p/Tip1p family ofserine-alanine-rich proteins; transcription isinduced by cold shock and anaerobiosis 0.000570.02724 S000005029MKT1YNL085WverifiedProtein that forms a complex with Pbp1p that may mediateposttranscriptional regulation of HOendonuclease; involved in propagation of M2dsRNA satellite of L-A virus 0.000570.02724 S000005724BFR1YOR198CverifiedComponent of mRNP complexes associated with polyribosomes;implicated in secretion and nuclearsegregation; multicopy suppressor of BFA(Brefeldin A) sensitivity 0.000570.02724&radic&radic S000005798YTM1YOR272WverifiedConstituent of 66S pre-ribosomal particles, required formaturation of the large ribosomal subunit 0.000570.02722 S000001526PHD1YKL043WverifiedTranscriptional activator that enhances pseudohyphal growth;regulates expression of FLO11, an adhesinrequired for pseudohyphal filament formation;similar to StuA, an A. nidulans developmentalregulator; potential Cdc28p substrate 0.000570.02722&radic&radic S000004842RRP5YMR229CverifiedProtein required for the synthesis of both 18S and 5.8S rRNA;C-terminal region is crucial for the formationof 18S rRNA and N-terminal region is requiredfor the 5.8S rRNA; component of small ribosomalsubunit (SSU) processosome 0.000570.02722 S000004155PUS5YLR165CverifiedPseudouridine synthase, catalyzes only the formation ofpseudouridine (Psi)-2819 in mitochondrial 21SrRNA; not essential for viability 0.000570.02722&radic&radic&radic S000003098CEG1YGL130WverifiedAlpha (guanylyltransferase) subunit of the mRNA capping enzyme,a heterodimer (the other subunit is CET1, anRNA 5'-triphophatase) involved in adding the 5'cap to mRNA; the mammalian enzyme is a singlebifunctional polypeptide 0.000570.02722 S000005732NOC2YOR206WverifiedProtein that forms a nucleolar complex with Mak21p that bindsto 90S and 66S pre-ribosomes, as well as anuclear complex with Noc3p that binds to 66Spre-ribosomes; both complexes mediateintranuclear transport of ribosomal precursors 0.000570.02717 S000003046DBP3YGL078CverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in ribosomal biogenesis 0.000570.02717 S000000456DUT1YBR252WverifieddUTPase, catalyzes hydrolysis of dUTP to dUMP and PPi, therebypreventing incorporation of uracil into DNAduring replication; critical for themaintenance of genetic stability 0.000570.02717&radic&radic S000000564KRR1YCL059CverifiedEssential nucleolar protein required for the synthesis of 18SrRNA and for the assembly of 40S ribosomalsubunit 0.000570.02717 S000005604BUD21YOR078WverifiedComponent of small ribosomal subunit (SSU) processosome thatcontains U3 snoRNA; originally isolated asbud-site selection mutant that displays arandom budding pattern 0.000570.02717 S000002408NTH1YDR001CverifiedNeutral trehalase, degrades trehalose; required forthermotolerance and may mediate resistance toother cellular stresses; may be phosphorylatedby Cdc28p 0.000570.02717 S000003681SFH5YJL145WverifiedPutative phosphatidylinositol transfer protein (PITP), exhibitsphosphatidylinositol- but notphosphatidylcholine-transfer activity, mainlylocalized to cytosol and microsomes, similar toSec14p; may be PITP regulator rather thanactual PITP 0.000570.02708&radic&radic S000002302CCT4YDL143WverifiedSubunit of the cytosolic chaperonin Cct ring complex, relatedto Tcp1p, required for the assembly of actinand tubulins in vivo 0.000570.02708&radic&radic S000001109HTD2YHR067WverifiedMitochondrial 3-hydroxyacyl-thioester dehydratase involved infatty acid biosynthesis, required forrespiratory growth and for normal mitochondrialmorphology 0.000570.02708&radic&radic S000000297PHO5YBR093CverifiedRepressible acid phosphatase (1 of 3) that also mediatesextracellular nucleotide-derived phosphatehydrolysis; secretory pathway derived cellsurface glycoprotein; induced by phosphatestarvation and coordinately regulated by PHO4and PHO2 0.000570.02708&radic&radic S000004829SKY1YMR216CverifiedSR protein kinase (SRPK) involved in regulating proteinsinvolved in mRNA metabolism and cationhomeostasis; similar to human SRPK1 0.000570.02708&radic&radic S000003530MRS11YHR005C-AverifiedEssential protein of the mitochondrial intermembrane space,forms a complex with Tim9p (TIM10 complex) thatmediates insertion of hydrophobic proteins atthe inner membrane, has homology to Mrs5p,which is also involved in this process 0.000570.02708&radic&radic S000002688RRP45YDR280WverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.000570.02708 S000002285PCL2YDL127WverifiedG1 cyclin, associates with Pho85p cyclin-dependent kinase (Cdk)to contribute to entry into the mitotic cellcycle, essential for cell morphogenesis;localizes to sites of polarized cell growth 0.000570.02703&radic&radic S000002389PTP1YDL230WverifiedPhosphotyrosine-specific protein phosphatase thatdephosphorylates a broad range of substrates invivo, including Fpr3p; localized to thecytoplasm and the mitochondria 0.000560.02682&radic&radic S000006214RPA135YPR010CverifiedRNA polymerase I subunit A135 0.000560.02682 S000001105PAN5YHR063Cverified2-dehydropantoate 2-reductase, part of the pantothenic acidpathway, structurally homologous to E. colipanE 0.000560.02682&radic S000006359NCA2YPR155CverifiedProtein involved in regulation of mitochondrial expression ofsubunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1ATP synthase; functions with Nca3p 0.000560.02682&radic&radic S000003207CSE1YGL238WverifiedNuclear envelope protein that mediates the nuclear export ofimportin alpha (Srp1p), homolog of metazoan CASprotein, required for accurate chromosomesegregation 0.000560.02682&radic&radic&radic S000006385SEC23YPR181CverifiedGTPase-activating protein; component of the Sec23p-Sec24pheterodimeric complex of the COPII vesiclecoat, involved in ER to Golgi transport andautophagy; stimulates the GDP-bound form ofSar1p 0.000560.02682 S000003823NTA1YJR062CverifiedAmidase, removes the amide group from N-terminal asparagine andglutamine residues to generate proteins withN-terminal aspartate and glutamate residuesthat are targets of ubiquitin-mediateddegradation 0.000560.02682&radic S000003742NCE101YJL205CverifiedProtein of unknown function, involved in secretion of proteinsthat lack classical secretory signal sequences 0.000560.02682&radic S000000286UBC4YBR082CverifiedUbiquitin-conjugating enzyme that mediates degradation ofshort-lived and abnormal proteins; interactswith E3-CaM in ubiquitinating calmodulin;interacts with many SCF ubiquitin proteinligases; component of the cellular stressresponse 0.000560.02682 S000005502RRP40YOL142WverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.000560.02682 S000005810HUA2YOR284WuncharacterizedCytoplasmic protein of unknown function; computational analysisof large-scale protein-protein interaction datasuggests a possible role in actin patchassembly 0.000560.02682&radic&radic S000002303YDL144CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene. 0.000560.02682&radic&radic S000003602MPM1YJL066CverifiedMitochondrial membrane protein of unknown function, contains nohydrophobic stretches 0.000560.02682&radic&radic S000000435SWC5YBR231CuncharacterizedProtein of unknown function, component of the SWR1 complex,which exchanges histone variant H2AZ (Htz1p)for chromatin-bound histone H2A 0.000560.02682 S000003272KSS1YGR040WverifiedMitogen-activated protein kinase (MAPK) involved in signaltransduction pathways that control filamentousgrowth and pheromone response 0.000560.02682&radic S000002743MSN5YDR335WverifiedKaryopherin involved in nuclear import and export; shown to beresponsible for nuclear import of replicationprotein A and for export of several proteinsincluding Swi6p, Far1p, and Pho4p; cargodissociation involves binding to RanGTP 0.000560.02681&radic&radic S000005337ESF2YNR054CverifiedEssential nucleolar protein involved in pre-18S rRNAprocessing; component of the small subunit(SSU) processome; has sequence similarity tomABT1, a mouse transcription activator 0.000560.02681 S000003509YGR277CUncharacterizedPutative protein of unknown function; sequence similarity to Pantetheine-phosphate adenylyltransferase (PPAT); YGR277C is an essential gene 0.000560.02659&radic S000005923SNF8YPL002CverifiedComponent of the ESCRT-II complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; appears to be functionallyrelated to SNF7; involved in glucosederepression 0.000550.02659 S000001508PAN3YKL025CverifiedEssential subunit of the Pan2p-Pan3p poly(A)-ribonucleasecomplex, which acts to control poly(A) taillength and regulate the stoichiometry andactivity of postreplication repair complexes 0.000560.02659 S000001236EGD2YHR193CverifiedAlpha subunit of the heteromeric nascent polypeptide-associatedcomplex (NAC) involved in protein sorting andtranslocation, associated with cytoplasmicribosomes 0.000560.02659 S000003427SKI6YGR195Wverified3'-to-5' phosphorolytic exoribonuclease that is a subunit ofthe exosome; required for 3' processing of the5.8S rRNA; involved in 3' to 5' mRNAdegradation and translation inhibition ofnon-poly(A) mRNAs 0.000550.02659 S000005216SEC2YNL272CverifiedGuanyl-nucleotide exchange factor for the small G-proteinSec4p, located on cytoplasmic vesicles;essential for post-Golgi vesicle transport 0.000550.02659&radic&radic S000003606YJL070CUncharacterizedPutative protein of unknown function with similarity to AMP deaminases; YJL070C is a non-essential gene 0.000560.02659&radic&radic S000001087YHR045WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum 0.000560.02659&radic&radic S000003837CDC11YJR076CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM 0.000560.02659 S000002657YDR249CUncharacterizedHypothetical protein 0.000560.02659 S000000827GCD11YER025WverifiedGamma subunit of the translation initiation factor eIF2,involved in the identification of the startcodon; binds GTP when forming the ternarycomplex with GTP and tRNAi-Met 0.000560.02659&radic&radic S000003211YGL242CUncharacterizedPutative protein of unknown function; deletion mutant is viable 0.000550.02659&radic&radic S000003095SOH1YGL127CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; involved in telomeremaintenance; conserved with other metazoanMED31 subunits 0.000550.02659 S000003661GCD14YJL125CverifiedSubunit of tRNA (1-methyladenosine) methyltransferase, withGcd10p, required for the modification of theadenine at position 58 in tRNAs, especiallytRNAi-Met; first identified as a negativeregulator of GCN4 expression 0.000560.02659&radic S000006259SEC8YPR055WverifiedEssential 121kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 0.000560.02659&radic S000004918ADH2YMR303CverifiedGlucose-repressible alcohol dehydrogenase II, catalyzes theconversion of ethanol to acetaldehyde; involvedin the production of certain carboxylateesters; regulated by ADR1 0.000560.02659 S000000174ATG8YBL078CverifiedProtein required for autophagy; modified by the serial actionof Atg4p, Atg7p, and Atg3p, and conjugated atthe C terminus with phosphatidylethanolamine,to become the form essential for generation ofautophagosomes 0.000560.02659&radic&radic&radic S000005752ISU2YOR226CverifiedConserved protein of the mitochondrial matrix, required forsynthesis of mitochondrial and cytosoliciron-sulfur proteins, performs a scaffoldingfunction in mitochondria during Fe/S clusterassembly; isu1 isu2 double mutant is inviable 0.000550.02659&radic S000003693FAR1YJL157CverifiedCyclin-dependent kinase inhibitor that mediates cell cyclearrest in response to pheromone; also forms acomplex with Cdc24p, Ste4p, and Ste18p that mayspecify the direction of polarized growthduring mating; potential Cdc28p substrate 0.000560.02659&radic&radic S000002637IVY1YDR229WverifiedPhospholipid-binding protein that interacts with both Ypt7p andVps33p, may partially counteract the action ofVps33p and vice versa, localizes to the rim ofthe vacuole as cells approach stationary phase 0.000550.0265&radic&radic S000003483YGR251WUncharacterizedPutative protein of unknown function; deletion mutant has defects in pre-rRNA processing; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the nucleolus; YGR251W is an essential gene 0.000550.0265 S000006047NAN1YPL126WverifiedU3 snoRNP protein, component of the small (ribosomal) subunit(SSU) processosome containing U3 snoRNA;required for the biogenesis of18S rRNA 0.000550.0265 S000001094CIC1YHR052WverifiedEssential protein that interacts with proteasome components andhas a potential role in proteasome substratespecificity; also copurifies with 66Spre-ribosomal particles 0.000550.0265 S000002211PBP4YDL053CverifiedPbp1p binding protein, interacts strongly with Pab1p-bindingprotein 1 (Pbp1p) in the yeast two-hybridsystem; also interacts with Lsm12p in acopurification assay 0.000550.0265&radic&radic S000001051YHR009CUncharacterizedPutative protein of unknown function; not an essential gene 0.000550.0265&radic&radic&radic S000003313SLX9YGR081CverifiedProtein of unknown function; deletion mutant has syntheticfitness defect with an sgs1 deletion mutant 0.000550.02625 S000002636PCF11YDR228CverifiedmRNA 3' end processing factor, essential component of cleavageand polyadenylation factor IA (CF IA), involvedin pre-mRNA 3' end processing and intranscription termination; binds C-terminaldomain of largest subunit of RNA pol II(Rpo21p) 0.000550.02625&radic&radic S000006067NOP53YPL146CverifiedNucleolar protein; involved in biogenesis of the 60S subunit ofthe ribosome; interacts with rRNA processingfactors Cbf5p and Nop2p; null mutant is viablebut growth is severely impaired 0.000550.02625 S000003496MES1YGR264CverifiedMethionyl-tRNA synthetase, forms a complex with glutamyl-tRNAsynthetase (Gus1p) and Arc1p, which increasesthe catalytic efficiency of both tRNAsynthetases; also has a role in nuclear exportof tRNAs 0.000550.02625 S000000918SLX8YER116CverifiedProtein containing a RING finger domain that interacts withHex3p; mutant phenotypes and geneticinteractions suggest a role in sumoylation andin genome stability 0.000550.02625 S000006093COX10YPL172CverifiedHeme A:farnesyltransferase, catalyzes the first step in theconversion of protoheme to the heme Aprosthetic group required for cytochrome coxidase activity; human ortholog is associatedwith mitochondrial disorders 0.000550.02625&radic&radic S000005995YTA6YPL074WverifiedPutative ATPase of the CDC48/PAS1/SEC18 (AAA) family, localizedto the cortex of mother cells but not todaughter cells 0.000550.02625 S000002444KRS1YDR037WverifiedLysyl-tRNA synthetase; also identified as a negative regulatorof general control of amino acid biosynthesis 0.000550.02625&radic&radic S000003956IRC19YLL033WuncharacterizedPutative protein of unknown function; YLL033W is not anessential gene but mutant is defective in sporeformation; null mutant displays increasedlevels of spontaneous Rad52 foci 0.000550.02625&radic&radic S000004523SML1YML058WverifiedRibonucleotide reductase inhibitor involved in regulating dNTPproduction; regulated by Mec1p and Rad53pduring DNA damage and S phase 0.000550.02625 S000005506PSF3YOL146WverifiedSubunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), whichis localized to DNA replication origins andimplicated in assembly of the DNA replicationmachinery 0.000550.02625&radic&radic S000001694TRP3YKL211CverifiedBifunctional enzyme exhibiting both indole-3-glycerol-phosphatesynthase and anthranilate synthase activities,forms multifunctional hetero-oligomericanthranilate synthase:indole-3-glycerolphosphate synthase enzyme complex with Trp2p 0.000550.02625&radic&radic S000004467ERG6YML008CverifiedDelta(24)-sterol C-methyltransferase, converts zymosterol tofecosterol in the ergosterol biosyntheticpathway by methylating position C-24; localizedto both lipid particles and mitochondrial outermembrane 0.000540.02625&radic S000002536SAC6YDR129CverifiedFimbrin, actin-bundling protein; cooperates with Scp1p(calponin/transgelin) in the organization andmaintenance of the actin cytoskeleton 0.000550.02625 S000002390BRE4YDL231CverifiedZinc finger protein containing five transmembrane domains; nullmutant exhibits strongly fragmented vacuolesand sensitivity to brefeldin A, a drug which isknown to affect intracellular transport 0.000540.02625&radic&radic S000004741YMR134WUncharacterizedPutative protein of unknown function that is highly conserved in ascomycetes and may be involved in mitochondrial function; localized to the endoplasmic reticulum; YMR134W is an essential gene 0.000540.02625&radic&radic S000000779MAK10YEL053CverifiedNon-catalytic subunit of N-terminal acetyltransferase of theNatC type, required for replication of dsRNAvirus; expression is glucose-repressible 0.000550.02625 S000004264CDC46YLR274WverifiedComponent of the hexameric MCM complex, which is important forpriming origins of DNA replication in G1 andbecomes an active ATP-dependent helicase thatpromotes DNA melting and elongation whenactivated by Cdc7p-Dbf4p in S-phase 0.000550.02625 S000001907YFR011CUncharacterizedPutative protein of unknown function; localizes to the mitochondrion; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR011C is not an essential gene 0.000540.0261 S000005440REX4YOL080CverifiedPutative RNA exonuclease possibly involved in pre-rRNAprocessing and ribosome assembly 0.000540.0261 S000003954GPI13YLL031CverifiedER membrane localized phosphoryltransferase that addsphosphoethanolamine onto the third mannoseresidue of the glycosylphosphatidylinositol(GPI) anchor precursor; similar to human PIG-Oprotein 0.000540.0261&radic&radic S000003247YGR015CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion 0.000540.0261&radic S000005955YPL034WUncharacterizedHypothetical protein 0.000540.0261&radic S000004117APC2YLR127CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; similar tocullin Cdc53p 0.000540.0261&radic S000000116RFT1YBL020WverifiedFlippase, essential integral membrane protein that is requiredfor translocation of Man5GlcNac2-PP-Dol fromthe cytoplasmic side to the lumenal side of theER membrane; mutation is suppressed byexpression human p53 protein 0.000540.0261&radic&radic S000001131GAR1YHR089CverifiedProtein component of the H/ACA snoRNP pseudouridylase complex,involved in the modification and cleavage ofthe 18S pre-rRNA 0.000540.0261 S000003931DRS1YLL008WverifiedNucleolar DEAD-box protein required for ribosome assembly andfunction, including synthesis of 60S ribosomalsubunits; constituent of 66S pre-ribosomalparticles 0.000540.0261&radic S000003632MRPL49YJL096WverifiedMitochondrial ribosomal protein of the large subunit 0.000540.0261&radic&radic S000002744YDR336WUncharacterizedPutative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene 0.000540.0261&radic&radic&radic S000003210KAP114YGL241WverifiedKaryopherin, responsible for nuclear import of Spt15p, histonesH2A and H2B, and Nap1p; amino terminus showssimilarity to those of other importins,particularly Cse1p; localization is primarilynuclear 0.000540.02598&radic&radic S000007608YGR271C-AUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus 0.000540.02598 S000004630TAP42YMR028WverifiedEssential protein involved in the TOR signaling pathway;physically associates with the proteinphosphatase 2A and the SIT4 protein phosphatasecatalytic subunits 0.000540.02598&radic S000005964NOP4YPL043WverifiedNucleolar protein, essential for processing and maturation of27S pre-rRNA and large ribosomal subunitbiogenesis; constituent of 66S pre-ribosomalparticles; contains four RNA recognition motifs(RRMs) 0.000540.02598 S000002272YDL114WUncharacterizedPutative protein of unknown function with similarity to acyl-carrier-protein reductases; YDL114W is not an essential gene 0.000540.02598&radic S000003454PET54YGR222WverifiedProtein required for splicing of the COX1 intron AI5 beta; alsospecifically required, together with Pet122pand Pet494p, for translation of the COX3 mRNA;located in the mitochondrial inner membrane 0.000540.02598&radic&radic S000005686MTR10YOR160WverifiedNuclear import receptor, mediates the nuclear localization ofproteins involved in mRNA-nucleus export 0.000540.02596&radic S000003570HCA4YJL033WverifiedPutative nucleolar DEAD box RNA helicase; high-copy numbersuppression of a U14 snoRNA processing mutantsuggests an involvement in 18S rRNA synthesis 0.000540.02596 S000003400YGR168CUncharacterizedPutative protein of unknown function; YGR168C is not an essential gene 0.000530.02579&radic&radic S000001086DOG1YHR044Cverified2-deoxyglucose-6-phosphate phosphatase, similar to Dog2p,member of a family of low molecular weightphosphatases; confers 2-deoxyglucose resistancewhen overexpressed, in vivo substrate has notyet been identified 0.000530.02579&radic&radic S000005192RPA49YNL248CverifiedRNA polymerase I subunit A49 0.000530.02579 S000001123LRP1YHR081WverifiedSubstrate-specific nuclear cofactor for exosome activity in theprocessing of stable RNAs; required fortelomere length maintenance; homolog ofmammalian nuclear matrix protein C1D involvedin regulation of DNA repair and recombination 0.000530.02579 S000001338SEC28YIL076WverifiedEpsilon-COP subunit of the coatomer; regulates retrogradeGolgi-to-ER protein traffic; stabilizes Cop1p,the alpha-COP and the coatomer complex;non-essential for cell growth 0.000540.02579 S000004041FCF2YLR051CverifiedEssential nucleolar protein involved in the early steps of 35SrRNA processing; interacts with Faf1p; memberof a transcriptionally co-regulated set ofgenes called the RRB regulon 0.000530.02579 S000006256NHP6AYPR052CverifiedHigh-mobility group non-histone chromatin protein, functionallyredundant with Nhp6Bp; homologous to mammalianhigh mobility group proteins 1 and 2; acts torecruit transcription factor Rcs1p to certainpromoters 0.000530.02566&radic S000003196VID30YGL227WverifiedProtein involved in proteasome-dependent catabolite degradationof fructose-1,6-bisphosphatase (FBPase); shiftsthe balance of nitrogen metabolism toward theproduction of glutamate; localized to thenucleus and the cytoplasm 0.000530.02566&radic S000004883RRN9YMR270CverifiedProtein involved in promoting high level transcription of rDNA,subunit of UAF (upstream activation factor) forRNA polymerase I 0.000530.02566 S000005260PHA2YNL316CverifiedPrephenate dehydratase, catalyzes the conversion of prephanateto phenylpyruvate, which is a step in thephenylalanine biosynthesis pathway 0.000530.02566&radic&radic S000003079NSA1YGL111WverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis 0.000530.02566 S000007522YBR085C-AUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus 0.000530.02566&radic&radic S000006295YPR091CUncharacterizedHypothetical protein 0.000530.02566&radic&radic S000004507RRN11YML043CverifiedProtein required for rDNA transcription by RNA polymerase I,component of the core factor (CF) of rDNAtranscription factor, which also contains Rrn6pand Rrn7p 0.000530.02566 S000002865TOM1YDR457WverifiedE3 ubiquitin ligase of the hect-domain class; has a role inmRNA export from the nucleus and may regulatetranscriptional coactivators 0.000520.02536 S000001028MUP3YHL036WverifiedLow affinity methionine permease, similar to Mup1p 0.000520.02536&radic&radic S000006111NAB3YPL190CverifiedSingle stranded DNA binding protein; acidic ribonucleoprotein;required for termination of non-poly(A)transcripts and efficient splicing; interactswith Nrd1p 0.000530.02536 S000004546YML081WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YML081w is not an essential gene 0.000530.02536&radic S000000119MCM2YBL023CverifiedProtein involved in DNA replication; component of the Mcm2-7hexameric complex that binds chromatin as apart of the pre-replicative complex 0.000530.02536&radic&radic S000001069RPN1YHR027CverifiedNon-ATPase base subunit of the 19S regulatory particle of the26S proteasome; may participate in therecognition of several ligands of theproteasome; contains a leucine-rich repeat(LRR) domain, a site for protein?proteininteractions 0.000530.02536&radic&radic&radic S000001597APN1YKL114CverifiedMajor apurinic/apyrimidinic endonuclease, 3'-repair diesteraseinvolved in repair of DNA damage by oxidationand alkylating agents; also functions as a3'-5' exonuclease to repair7,8-dihydro-8-oxodeoxyguanosine 0.000530.02536 S000003612NET1YJL076WverifiedCore subunit of the RENT complex, which is a complex involvedin nucleolar silencing and telophase exit;stimulates transcription by RNA polymerase Iand regulates nucleolar structure 0.000530.02536 S000001760MRS4YKR052CverifiedMitochondrial iron transporter of the mitochondrial carrierfamily (MCF), very similar to and functionallyredundant with Mrs3p; functions under low-ironconditions; may transport other cations inaddition to iron 0.000520.02536&radic S000003992NOC3YLR002CverifiedProtein that forms a nuclear complex with Noc2p that binds to66S ribosomal precursors to mediate theirintranuclear transport; also binds to chromatinto promote the association of DNA replicationfactors and replication initiation 0.000520.02536 S000004614CLU1YMR012WverifiedeIF3 component of unknown function; deletion causes defects inmitochondrial organization but not in growth ortranslation initiation, can rescue cytokinesisand mitochondrial organization defects of theDictyostelium cluA- mutant 0.000520.02536 S000002186MPS1YDL028CverifiedDual-specificity kinase required for spindle pole body (SPB)duplication and spindle checkpoint function;substrates include SPB proteins Spc42p,Spc110p, and Spc98p, mitotic exit networkprotein Mob1p, and checkpoint protein Mad1p 0.000530.02536&radic&radic S000004670SOV1YMR066WuncharacterizedMitochondrial protein of unknown function 0.000530.02536&radic S000004211RSA3YLR221CverifiedProtein with a likely role in ribosomal maturation, requiredfor accumulation of wild-type levels of large(60S) ribosomal subunits; binds to the helicaseDbp6p in pre-60S ribosomal particles in thenucleolus 0.000520.02536 S000004830GUA1YMR217WverifiedGMP synthase, an enzyme that catalyzes the second step in thebiosynthesis of GMP from inosine 5'-phosphate(IMP); transcription is not subject toregulation by guanine but is negativelyregulated by nutrient starvation 0.000520.02526&radic&radic S000003883CAF17YJR122WverifiedMitochondrial protein that interacts with Ccr4p in thetwo-hybrid system; 3'-untranslated regioncontains a putative mRNA localization elementcommon to genes encoding mitochondrial proteins 0.000520.02526&radic S000001409SLN1YIL147CverifiedHistidine kinase osmosensor that regulates a MAP kinasecascade; transmembrane protein with anintracellular kinase domain that signals toYpd1p and Ssk1p, thereby forming a phosphorelaysystem similar to bacterial two-componentregulators 0.000520.02526&radic S000000163UBP13YBL067CverifiedPutative ubiquitin-specific protease 0.000520.02526&radic S000001718TOF2YKR010CverifiedNonessential mitochondrial protein of unknown function withsequence similarity to Net1p; identified as atopoisomerase I (Top1p) binding protein;displays synthetic genetic interactions withTOP1 and HPR1 &radic0.000520.02525&radic&radic S000003413TIM13YGR181WverifiedMitochondrial intermembrane space protein, forms a complex withTIm8p that mediates import and insertion of asubset of polytopic inner membrane proteins;may prevent aggregation of incoming proteins ina chaperone-like manner 0.000520.02525&radic&radic S000003085YGL117WUncharacterizedPutative protein of unknown function 0.000520.02525&radic&radic&radic S000004262YCS4YLR272CverifiedNon-SMC subunit of the condensin complex(Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required forestablishment and maintenance of chromosomecondensation, chromosome segregation, chromatinbinding of condensin and silencing at themating type locus 0.000520.02525 S000003065SRM1YGL097WverifiedNucleotide exchange factor for Gsp1p, localizes to the nucleus,required for nucleocytoplasmic trafficking ofmacromolecules; potentially phosphorylated byCdc28p 0.000520.02512&radic&radic S000001104RPP1YHR062CverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.000520.02512&radic S000004437YLR445WUncharacterizedHypothetical protein 0.000520.02512&radic&radic S000005457WRS1YOL097CverifiedCytoplasmic tryptophanyl-tRNA synthetase, aminoacylatestryptophanyl-tRNA 0.000520.02512&radic&radic S000005535TIR4YOR009WverifiedCell wall mannoprotein of the Srp1p/Tip1p family ofserine-alanine-rich proteins; expressed underanaerobic conditions and required for anaerobicgrowth; transcription is also induced by coldshock 0.000520.02512 S000003136HUR1YGL168WuncharacterizedProtein required for hydroxyurea resistance; has possible rolesin DNA replication and maintenance of propertelomere length 0.000520.02512&radic&radic&radic S000000702ADH7YCR105WverifiedNADPH-dependent cinnamyl alcohol dehydrogenase family memberwith broad substrate specificity; may beinvolved in fusel alcohol synthesis 0.000510.0246&radic S000004070EMP46YLR080WverifiedIntegral membrane component of endoplasmic reticulum-derivedCOPII-coated vesicles, which function in ER toGolgi transport 0.000510.0246&radic&radic S000000089YAR064WUncharacterizedPutative protein of unknown function 0.000510.0246&radic&radic&radic S000002881PRP3YDR473CverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.000520.0246&radic S000004840TAF7YMR227CverifiedTFIID subunit (67 kDa), involved in RNA polymerase IItranscription initiation 0.000510.0246&radic S000000559SPB1YCL054WverifiedAdoMet-dependent methyltransferase involved in rRNA processingand 60S ribosomal subunit maturation;methylates G2922 in the tRNA docking site ofthe large subunit rRNA and in the absence ofsnR52, U2921; suppressor of PAB1 mutants 0.000510.0246&radic&radic&radic S000004781YMR171CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to endosomes; mRNA is targeted to the bud via the mRNA transport system involving She2p: YMR171C is not an essential gene 0.000510.0246&radic&radic S000000744EAF5YEL018WuncharacterizedEsa1p-associated factor, subunit of the NuA4 acetyltransferasecomplex 0.000510.0246 S000002421RAD61YDR014WverifiedProtein of unknown function; mutation confers radiationsensitivity 0.000510.0246&radic&radic&radic S000000451ENP1YBR247CverifiedProtein associated with U3 and U14 snoRNAs, required forpre-rRNA processing and 40S ribosomal subunitsynthesis; localized in the nucleus andconcentrated in the nucleolus 0.000510.0246 S000005119NOP13YNL175CverifiedProtein of unknown function, localizes to the nucleolus andnucleoplasm; contains an RNA recognition motif(RRM) and has similarity to Nop12p, which isrequired for processing of pre-18S rRNA 0.000520.0246 S000002443EHD3YDR036CverifiedProtein of unconfirmed function, plays an indirect role inendocytic membrane trafficking, member of afamily of enoyl-CoA hydratase/isomerases; thenon-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 0.000510.0246&radic&radic S000005736RPB10YOR210WverifiedRNA polymerase subunit ABC10-beta, common to RNA polymerases I,II, and III 0.000510.0246 S000003215RAI1YGL246CverifiedNuclear protein that binds to and stabilizes theexoribonuclease Rat1p, required for pre-rRNAprocessing 0.000510.0246&radic&radic S000000667IMG2YCR071CverifiedMitochondrial ribosomal protein of the small subunit 0.000510.0246&radic&radic&radic S000006082BEM4YPL161CverifiedProtein involved in establishment of cell polarity and budemergence; interacts with the Rho1p smallGTP-binding protein and with the Rho-typeGTPase Cdc42p; involved in maintenance ofproper telomere length 0.000510.0246 S000004697NPL6YMR091CverifiedComponent of the RSC chromatin remodeling complex; interactswith Rsc3p, Rsc30p, Ldb7p, and Htl1p to form amodule important for a broad range of RSCfunctions; involved in nuclear protein importand maintenance of proper telomere length 0.000520.0246&radic&radic S000004274ECI1YLR284CverifiedPeroxisomal delta3,delta2-enoyl-CoA isomerase, hexamericprotein that converts 3-hexenoyl-CoA totrans-2-hexenoyl-CoA, essential for thebeta-oxidation of unsaturated fatty acids,oleate-induced 0.000510.0246&radic&radic S000002251PMT5YDL093WverifiedProtein O-mannosyltransferase, transfers mannose residues fromdolichyl phosphate-D-mannose to proteinserine/threonine residues; acts in a complexwith Pmt3p, can instead interact with Pmt2p insome conditions; target for new antifungals 0.000520.0246&radic&radic&radic S000004806MRPL24YMR193WverifiedMitochondrial ribosomal protein of the large subunit 0.000510.0246 S000001249SKN7YHR206WverifiedNuclear response regulator and transcription factor, part of abranched two-component signaling system;required for optimal induction of heat-shockgenes in response to oxidative stress; involvedin osmoregulation 0.000510.0246 S000002718SUM1YDR310CverifiedTranscriptional repressor required for mitotic repression ofmiddle sporulation-specific genes; involved intelomere maintenance, regulated by thepachytene checkpoint 0.000510.0246&radic S000001064YHR022CUncharacterizedPutative protein of unknown function; YHR022C is not an essential gene. 0.000520.0246&radic&radic S000000460RIB5YBR256CverifiedRiboflavin synthase; catalyzes the last step of the riboflavinbiosynthesis pathway 0.000510.0246&radic&radic S000003645UTP10YJL109CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000510.02459 S000001915FAB1YFR019Wverified1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membranekinase that generates phosphatidylinositol(3,5)P2, which is involved in vacuolar sortingand homeostasis 0.000510.02459&radic S000003633PHS1YJL097WuncharacterizedProtein of unknown function; homolog of mammalian PTPLA;involved in sphingolipid biosynthesis, proteintrafficking; required for cell viability 0.00050.02406&radic&radic S000003326VAS1YGR094WverifiedMitochondrial and cytoplasmic valyl-tRNA synthetase 0.000510.02406&radic&radic S000002940YDR532CUncharacterizedProtein of unknown function that localizes to the nuclear side of the spindle pole body and along short spindles; deletion mutants have short telomeres; forms a complex with Spc105p 0.00050.02406 S000002449YDR042CUncharacterizedHypothetical protein 0.000510.02406&radic S000004410CDC73YLR418CverifiedConstituent of Paf1 complex with RNA polymerase II, Paf1p,Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinctfrom Srb-containing Pol II complexes; requiredfor expression of certain genes, modificationof some histones, and telomere maintenance 0.00050.02406&radic S000003623TRL1YJL087CverifiedtRNA ligase, required for tRNA splicing; composed of threeessential domains containing thephosphodiesterase, polynucleotide kinase, andligase activities required for ligation;localized at the inner membrane of the nuclearenvelope 0.000510.02406&radic&radic S000005685SME1YOR159CverifiedCore Sm protein Sm E; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm E 0.00050.02406&radic S000003439YGR207CVerifiedProtein of unknown function; homolog of mammalian electron transfer flavoprotein complex subunit ETF-beta; interacts with frataxin (Yfh1p) 0.000490.02252&radic S000003746PEX2YJL210WverifiedRING-finger peroxin, peroxisomal membrane protein with aC-terminal zinc-binding RING domain, formsputative translocation subcomplex with Pex10pand Pex12p which functions in peroxisomalmatrix protein import 0.000490.02252 S000001552YKL069WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.000490.02252&radic&radic S000001070DAP2YHR028CverifiedDipeptidyl aminopeptidase, synthesized as a glycosylatedprecursor; localizes to the vacuolar membrane;similar to Ste13p 0.00050.02252&radic&radic&radic S000002835RPN9YDR427WverifiedNon-ATPase regulatory subunit of the 26S proteasome, hassimilarity to putative proteasomal subunits inother species; null mutant is temperaturesensitive and exhibits cell cycle andproteasome assembly defects 0.00050.02252&radic&radic S000001416IMP2'YIL154CverifiedTranscriptional activator involved in maintenance of ionhomeostasis and protection against DNA damagecaused by bleomycin and other oxidants,contains a C-terminal leucine-rich repeat 0.000490.02252&radic&radic&radic S000005098YCK2YNL154CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck1p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck1p 0.00050.02252&radic&radic S000000056BDH1YAL060WverifiedNAD-dependent (2R,3R)-2,3-butanediol dehydrogenase, azinc-containing medium-chain alcoholdehydrogenase, produces 2,3-butanediol fromacetoin during fermentation and allows using2,3-butanediol as a carbon source duringaerobic growth 0.000490.02252&radic&radic S000003529QCR10YHR001W-AverifiedSubunit of the ubiqunol-cytochrome c oxidoreductase complex,involved in aerobic respiration 0.000490.02252&radic&radic S000003803NUP85YJR042WverifiedSubunit of the Nup84p subcomplex of the nuclear pore complex(NPC), required for assembly of the subcomplexand also for formation of the nucleocytoplasmicGsp1p concentration gradient that plays a rolein nuclear trafficking 0.00050.02252&radic S000001732DBP7YKR024CverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in ribosomal biogenesis 0.00050.02252 S000003006OCH1YGL038CverifiedMannosyltransferase of the cis-Golgi apparatus, initiates thepolymannose outer chain elongation of N-linkedoligosaccharides of glycoproteins 0.000490.02252&radic&radic S000005370RCL1YOL010WverifiedRNA terminal phosphate cyclase-like protein involved in rRNAprocessing at sites A0, A1, and A2; does notpossess detectable RNA cyclase activity 0.000490.02252 S000005837NOP58YOR310CverifiedProtein involved in pre-rRNA processing, 18S rRNA synthesis,and snoRNA synthesis; component of the smallsubunit processome complex, which is requiredfor processing of pre-18S rRNA 0.000490.02252 S000004783HOT1YMR172WverifiedTranscription factor required for the transient induction ofglycerol biosynthetic genes GPD1 and GPP2 inresponse to high osmolarity; targets Hog1p toosmostress responsive promoters; has similarityto Msn1p and Gcr1p 0.00050.02252&radic&radic S000005195NRD1YNL251CverifiedRNA-binding protein that interacts with the C-terminal domainof the RNA polymerase II large subunit(Rpo21p), required for transcriptiontermination and 3' end maturation ofnonpolyadenylated RNAs 0.00050.02252 S000003896MCM22YJR135CverifiedProtein involved in minichromosome maintenance; component ofthe kinetochore; binds to centromeric DNA in aCtf19p-dependent manner 0.000490.02252&radic S000000609YCR016WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; YCR016W is not an essential gene 0.00050.02252 S000002707BFR2YDR299WverifiedEssential protein possibly involved in secretion; multicopysuppressor of sensitivity to Brefeldin A 0.000490.02252 S000004275NNT1YLR285WverifiedPutative nicotinamide N-methyltransferase, has a role in rDNAsilencing and in lifespan determination 0.000490.02252&radic&radic S000003605UTP18YJL069CuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.000490.02252 S000003504YGR272CUncharacterizedPutative protein of unknown function; deletion mutant has defects in pre-rRNA processing; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.000490.02252&radic&radic S000003999RLP24YLR009WverifiedRibosomal Like Protein 24 0.000490.02252 S000002857UTP6YDR449CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000490.02252 S000001065MYO1YHR023WverifiedType II myosin heavy chain, required for wild-type cytokinesisand cell separation; localizes to theactomyosin ring; binds to myosin light chainsMlc1p and Mlc2p through its IQ1 and IQ2 motifsrespectively 0.000490.02252&radic&radic S000002247YDL089WUncharacterizedProtein of unknown function; interacts with meiotic division protein Csm1p; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery, potential Cdc28p substrate 0.000490.02252&radic S000002273IWR1YDL115CuncharacterizedProtein of unknown function, deletion causes hypersensitivityto the K1 killer toxin 0.00050.02252&radic S000000282ECM33YBR078WverifiedGPI-anchored protein of unknown function, has a possible rolein apical bud growth; GPI-anchoring on theplasma membrane crucial to function; similar toSps2p and Pst1p 0.000490.02252&radic S000001243RPN10YHR200WverifiedNon-ATPase base subunit of the 19S regulatory particle (RP) ofthe 26S proteasome; N-terminus plays a role inmaintaining the structural integrity of the RP;binds selectively to polyubiquitin chains;homolog of the mammalian S5a protein 0.00050.02252 S000004295ACO1YLR304CverifiedAconitase, required for the tricarboxylic acid (TCA) cycle andalso independently required for mitochondrialgenome maintenance; component of themitochondrial nucleoid; mutation leads toglutamate auxotrophy 0.00050.02252 S000003214GUS1YGL245WverifiedGlutamyl-tRNA synthetase (GluRS), forms a complex withmethionyl-tRNA synthetase (Mes1p) and Arc1p;complex formation increases the catalyticefficiency of both tRNA synthetases and ensurestheir correct localization to the cytoplasm 0.00050.02252&radic&radic S000004606MVP1YMR004WverifiedProtein required for sorting proteins to the vacuole;overproduction of Mvp1p suppresses severaldominant VPS1 mutations; Mvp1p and Vps1p act inconcert to promote membrane traffic to thevacuole 0.000490.02252&radic&radic S000004791SPT21YMR179WverifiedProtein required for normal transcription at several lociincluding HTA2-HTB2 and HHF2-HHT2, but notrequired at the other histone loci;functionally related to Spt10p; involved intelomere maintenance 0.00050.02252 S000005753YOR227WUncharacterizedHypothetical protein 0.000490.02252&radic S000005597THI71YOR071CuncharacterizedTransporter of thiamine or related compound; shares sequencesimilarity to Thi7p; proposed to be involved in5-fluorocytosine sensitivity 0.000490.02252&radic&radic S000004411YLR419WUncharacterizedPutative helicase with limited sequence similarity to human Rb protein; localized to the cytoplasm and the mitochondria; YLR419W is not an essential gene 0.000490.02238&radic S000005782TRE2YOR256CverifiedProtein of unknown function with similarity to TRE1/YPL176C;required for cell viability 0.000490.02238&radic&radic S000005242CLA4YNL298WverifiedCdc42p activated signal transducing kinase of the PAK(p21-activated kinase) family, involved inseptin ring assembly and cytokinesis; directlyphosphorylates septins Cdc3p and Cdc10p; otheryeast PAK family members are Ste20p and Skm1p 0.000490.02211 S000004559GIM5YML094WverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 0.000480.02211&radic&radic S000001045YHR003CVerifiedProtein of unknown function, localized to the mitochondrial outer membrane 0.000490.02211&radic S000000208GPI18YBR004CverifiedFunctional ortholog of human PIG-V, which is amannosyltransferase that transfers the secondmannose in glycosylphosphatidylinositolbiosynthesis; the authentic, non-tagged proteinwas localized to mitochondria 0.000480.02211&radic&radic S000004466YAP1YML007WverifiedBasic leucine zipper (bZIP) transcription factor required foroxidative stress tolerance; mediatespleiotropic drug and metal resistance;localized to the nucleus in response to thepresence of oxidants 0.000480.02211 S000001317YIL055CUncharacterizedHypothetical protein 0.000480.02211&radic&radic S000003949HSP104YLL026WverifiedHeat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p(Hsp70) to refold and reactivate previouslydenatured, aggregated proteins; responsive tostresses including: heat, ethanol, and sodiumarsenite; involved in [PSI+] propagation 0.000480.02184&radic S000002996SCW11YGL028CverifiedCell wall protein with similarity to glucanases; may play arole in conjugation during mating based on itsregulation by Ste12p 0.000480.02184&radic&radic S000004632RSF1YMR030WverifiedProtein required for respiratory growth; localized to both thenucleus and mitochondrion; mutant displaysdecreased transcription of specific nuclear andmitochondrial genes whose products are involvedin respiratory growth 0.000480.02184&radic S000003321NNF2YGR089WuncharacterizedProtein that exhibits physical and genetic interactions withRpb8p, which is a subunit of RNA polymerases I,II, and III; computational analysis oflarge-scale protein-protein interaction datasuggests a role in chromosome segregation 0.000480.02147&radic&radic S000000306EXO84YBR102CverifiedEssential protein with dual roles in spliceosome assembly andexocytosis; the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p) mediates polarized targeting ofsecretory vesicles to active sites ofexocytosis 0.000480.02147&radic S000003044RPL7AYGL076CverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl7Bp and has similarity to E.coli L30 and rat L7 ribosomal proteins;contains a conserved C-terminal Nucleic acidBinding Domain (NDB2) 0.000480.02147&radic S000003412RNR4YGR180CverifiedRibonucleotide-diphosphate reductase (RNR), small subunit; theRNR complex catalyzes the rate-limiting step indNTP synthesis and is regulated by DNAreplication and DNA damage checkpoint pathwaysvia localization of the small subunits 0.000480.02147 S000005996GCR1YPL075WverifiedTranscriptional activator of genes involved in glycolysis;DNA-binding protein that interacts andfunctions with the transcriptional activatorGcr2p 0.000480.02138&radic&radic S000004212UTP13YLR222CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000480.02053 S000001702COS9YKL219WverifiedProtein of unknown function, member of the DUP380 subfamily ofconserved, often subtelomerically-encodedproteins 0.000470.02053&radic&radic S000003951TPO1YLL028WverifiedPolyamine transporter that recognizes spermine, putrescine, andspermidine; catalyzes uptake of polyamines atalkaline pH and excretion at acidic pH;phosphorylation enhances activity and sortingto the plasma membrane 0.000480.02053&radic S000003719MNN11YJL183WverifiedSubunit of a Golgi mannosyltransferase complex that alsocontains Anp1p, Mnn9p, Mnn10p, and Hoc1p, andmediates elongation of the polysaccharidemannan backbone; has homology to Mnn10p 0.000470.02053 S000003495SAY1YGR263CuncharacterizedPutative protein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to theendoplasmic reticulum 0.000470.02053&radic S000003675YUR1YJL139CverifiedMannosyltransferase of the KTR1 family, involved in proteinN-glycosylation; located in the Golgi apparatus 0.000470.02053&radic&radic S000000209RCR1YBR005WverifiedProtein of the endoplasmic reticulum membrane involved inchitin deposition in the cell wall;overproduction confers resistance to Congo Red 0.000470.02053&radic&radic S000001292SSM4YIL030CverifiedUbiquitin-protein ligase of the ER/nuclear envelope, requiredfor degradation of Alpha2p and other proteinscontaining a Deg1 degradation signal; ssm4mutation suppresses mRNA instability caused byan rna14 mutation 0.000470.02053&radic&radic S000001608RRN3YKL125WverifiedProtein required for transcription of rDNA by RNA polymerase I;transcription factor independent of DNAtemplate; involved in recruitment of RNApolymerase I to rDNA 0.000470.02046 S000004987BOP3YNL042WverifiedProtein of unknown function, potential Cdc28p substrate;overproduction suppresses a pam1 slv3 doublenull mutation and confers resistance tomethylmercury 0.000470.02024&radic S000002905ITR1YDR497CverifiedMyo-inositol transporter with strong similarity to the minormyo-inositol transporter Itr2p, member of thesugar transporter superfamily; expression isrepressed by inositol and choline via Opi1p andderepressed via Ino2p and Ino4p 0.000470.02024 S000005343FRE4YNR060WverifiedFerric reductase, reduces a specific subset ofsiderophore-bound iron prior to uptake bytransporters; expression induced by low ironlevels 0.000470.02024&radic&radic S000002517FOB1YDR110WverifiedNucleolar protein required for DNA replication fork blockingand recombinational hotspot activities; bindsto the replication fork barrier site in therDNA region; related to retroviral integrases 0.000460.01984 S000005873REV1YOR346WverifiedDeoxycytidyl transferase, forms a complex with the subunits ofDNA polymerase zeta, Rev3p and Rev7p; involvedin repair of abasic sites in damaged DNA 0.000470.01984&radic&radic&radic S000001617OCT1YKL134CverifiedMitochondrial intermediate peptidase, cleaves N-terminalresidues of a subset of proteins upon import,after their cleavage by mitochondrialprocessing peptidase (Mas1p-Mas2p); maycontribute to mitochondrial iron homeostasis 0.000460.01984&radic S000001314RPL34BYIL052CverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl34Ap and has similarity to ratL34 ribosomal protein 0.000460.01984&radic&radic S000004024SMF3YLR034CverifiedPutative divalent metal ion transporter involved in ironhomeostasis; transcriptionally regulated bymetal ions; member of the Nramp family of metaltransport proteins 0.000460.01984&radic S000001313MMF1YIL051CverifiedMitochondrial protein involved in maintenance of themitochondrial genome 0.000460.01984&radic&radic S000006119RPL7BYPL198WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl7Ap and has similarity to E.coli L30 and rat L7 ribosomal proteins;contains a conserved C-terminal Nucleic acidBinding Domain (NDB2) 0.000460.01984 S000001836THI5YFL058WverifiedProtein involved in synthesis of the thiamine precursorhydroxymethylpyrimidine (HMP); member of asubtelomeric gene family including THI5, THI11,THI12, and THI13 0.000470.01984&radic S000004841MTF1YMR228WverifiedMitochondrial RNA polymerase specificity factor with structuralsimilarity to S-adenosylmethionine-dependentmethyltransferases and functional similarity tobacterial sigma-factors, interacts withmitochondrial core polymerase Rpo41p 0.000460.01984&radic S000002820RRP17YDR412WuncharacterizedProtein required for cell viability 0.000460.01984 S000000536RRP7YCL031CverifiedEssential protein involved in rRNA processing and ribosomebiogenesis 0.000460.01976 S000006000RPL21BYPL079WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl21Ap and has similarity to ratL21 ribosomal protein 0.000460.01976&radic&radic&radic S000005384YOL024WUncharacterizedHypothetical protein 0.000460.01955&radic S000001334HOP1YIL072WverifiedMeiosis-specific DNA binding protein that displays Red1pdependent localization to the unsynapsedaxial-lateral elements of the synaptonemalcomplex; required for homologous chromosomesynapsis and chiasma formation 0.000460.01955&radic&radic S000002668SWM1YDR260CverifiedSubunit of the anaphase-promoting complex, which is an E3ubiquitin ligase that regulates themetaphase-anaphase transition and exit frommitosis; required for activation of thedaughter-specific gene expression and sporewall maturation 0.000460.01955 S000004277YLR287CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene 0.000460.01955 S000001224SVP26YHR181WverifiedIntegral membrane protein of the early Golgi apparatus andendoplasmic reticulum, involved in COP IIvesicle transport; may also function to promoteretention of proteins in the early Golgicompartment 0.000450.01955&radic S000000307SIF2YBR103WverifiedWD40 repeat-containing subunit of the Set3C histone deacetylasecomplex, which represses early/middlesporulation genes; antagonizes telomericsilencing; binds specifically to the Sir4pN-terminus 0.000450.01935&radic S000003248YGR016WUncharacterizedHypothetical protein 0.000450.01935&radic S000005499CDC33YOL139CverifiedCytoplasmic mRNA cap binding protein; the eIF4E-cap complex isresponsible for mediating cap-dependent mRNAtranslation via interactions with thetranslation initiation factor eIF4G (Tif4631por Tif4632p) 0.000450.01935 S000003152STR3YGL184CverifiedCystathionine beta-lyase, converts cystathionine intohomocysteine 0.000450.01935&radic&radic S000006088REV3YPL167CverifiedCatalytic subunit of DNA polymerase zeta, which is involved inDNA repair and translesion synthesis; requiredfor mutagenesis induced by DNA damage 0.000450.01935&radic&radic&radic S000004021YLR031WUncharacterizedHypothetical protein 0.000450.01935&radic&radic&radic S000001332MAM33YIL070CverifiedAcidic protein of the mitochondrial matrix involved inoxidative phosphorylation; related to the humancomplement receptor gC1q-R 0.000450.01935&radic S000003702QCR8YJL166WverifiedSubunit 8 of ubiquinol cytochrome-c reductase complex, which isa component of the mitochondrial inner membraneelectron transport chain; oriented facing theintermembrane space; expression is regulated byAbf1p and Cpf1p 0.000450.01935&radic&radic S000003589PEP8YJL053WverifiedVacuolar protein sorting protein that forms part of themultimeric membrane-associated retromer complexalong with Vps35p, Vps29p, Vps17p, and Vps5p;essential for endosome-to-Golgi retrogradeprotein transport 0.000440.01907&radic S000000281SLM4YBR077CverifiedComponent of the EGO complex, which is involved in theregulation of microautophagy, and of the GSEcomplex, which is required for proper sortingof amino acid permease Gap1p; gene exhibitssynthetic genetic interaction with MSS4 0.000440.01907&radic S000004631FAR8YMR029CverifiedProtein involved in G1 cell cycle arrest in response topheromone, in a pathway different from theFar1p-dependent pathway; interacts with Far3p,Far7p, Far9p, Far10p, and Far11p 0.000440.0189&radic S000006368MMS1YPR164WverifiedProtein likely involved in protection againstreplication-dependent DNA damage; mutants aresensitive to methyl methanesulfonate (MMS);implicated in regulation of Ty1 transposition 0.000440.01888&radic S000001335SPO22YIL073CverifiedMeiosis-specific protein essential for chromosome synapsis,similar to phospholipase A2, involved incompletion of nuclear divisions during meiosis;induced early in meiosis 0.000430.01861&radic&radic S000004616BUD22YMR014WverifiedProtein involved in bud-site selection; diploid mutants displaya random budding pattern instead of thewild-type bipolar pattern 0.000430.01861 S000001333PCI8YIL071CverifiedPossible shared subunit of Cop9 signalosome (CSN) and eIF3,binds eIF3b subunit Prt1p, has possible dualfunctions in transcriptional and translationalcontrol, contains a PCI (Proteasome-COP9signalosome (CSN)-eIF3) domain 0.000430.01861&radic S000000602RVS161YCR009CverifiedAmphiphysin-like lipid raft protein; subunit of a complex(Rvs161p-Rvs167p) that regulates polarizationof the actin cytoskeleton, endocytosis, cellpolarity, cell fusion and viability followingstarvation or osmotic stress 0.000430.01861&radic S000004669KAR5YMR065WverifiedProtein required for nuclear membrane fusion during karyogamy,localizes to the membrane with a solubleportion in the endoplasmic reticulum lumen, mayform a complex with Jem1p and Kar2p; expressionof the gene is regulated by pheromone 0.000430.01861&radic S000000461POP4YBR257WverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends; binds to the RPR1 RNAsubunit in Rnase P 0.000430.01861&radic&radic S000000701PAU3YCR104WverifiedPart of 23-member seripauperin multigene family encoded mainlyin subtelomeric regions, active duringalcoholic fermentation, regulated byanaerobiosis, negatively regulated by oxygen,repressed by heme 0.000430.01847&radic&radic&radic S000000138FUI1YBL042CverifiedHigh affinity uridine permease, localized to the plasmamembrane; not involved in uracil transport 0.000420.01831&radic S000004029RIC1YLR039CverifiedProtein involved in retrograde transport to the cis-Golginetwork; forms heterodimer with Rgp1p that actsas a GTP exchange factor for Ypt6p; involved intranscription of rRNA and ribosomal proteingenes 0.000420.01831 S000003691FBP26YJL155CverifiedFructose-2,6-bisphosphatase, required for glucose metabolism 0.000420.01831&radic S000004995YNL050CUncharacterizedPutative protein of unknown function; YNL050c is not an essential gene 0.000420.01796&radic S000003815YJR054WUncharacterizedVacuolar protein of unknown function; potential Cdc28p substrate 0.000420.01796&radic S000000690CDC50YCR094WverifiedEndosomal protein that regulates cell polarity; similar toYnr048wp and Lem3p 0.000410.0177&radic&radic&radic S000003510CWC22YGR278WverifiedEssential protein, component of a complex containing Cef1p, hassimilarity to S. pombe Cwf22p; putativespliceosomal component, based on computationalanalysis of large-scale protein-proteininteraction data 0.000410.01722&radic S000003962UBI4YLL039CverifiedUbiquitin, becomes conjugated to proteins, marking them forselective degradation via the ubiquitin-26Sproteasome system; essential for the cellularstress response 0.000410.01722 S000004118DCN1YLR128WverifiedPutative Nedd8 ligase; binds Nedd8; involved in cullinneddylation; not essential; similar toC.elegans DCN-1; contains UBA-likeubiquitin-binding domain and a DUF298 domain 0.000410.01709&radic S000003865SOD1YJR104CverifiedCytosolic superoxide dismutase; some mutations are analogous tothose that cause ALS (amyotrophic lateralsclerosis) in humans 0.00040.01671&radic S000003521MAL11YGR289CverifiedMaltose permease, inducible high-affinity maltose transporter(alpha-glucoside transporter); encoded in theMAL1 complex locus; member of the 12transmembrane domain superfamily of sugartransporters 0.000390.01592&radic S000004971SAM50YNL026WverifiedEssential component of the Sorting and Assembly Machinery (SAMor Tob complex) of the mitochondrial outermembrane, which binds precursors of beta-barrelproteins and facilitates their outer membraneinsertion; homologous to bacterial Omp85 0.000390.01537&radic S000003102PCL10YGL134WverifiedPho85p cyclin; recruits, activates, and targets Pho85pcyclin-dependent protein kinase to itssubstrate 0.000390.01537&radic S000001110DYS1YHR068WverifiedDeoxyhypusine synthase, catalyzes formation of deoxyhypusine,the first step in hypusine biosynthesis;triggers posttranslational hypusination oftranslation elongation factor eIF-5A andregulates its intracellular levels; tetrameric 0.000380.01452&radic S000001939IRC6YFR043CuncharacterizedHypothetical protein; null mutant displays increased levels ofspontaneous Rad52 foci 0.000380.01452&radic S000002224IDP1YDL066WverifiedMitochondrial NADP-specific isocitrate dehydrogenase, catalyzesthe oxidation of isocitrate toalpha-ketoglutarate; not required formitochondrial respiration and may function todivert alpha-ketoglutarate to biosyntheticprocesses 0.000380.01452&radic&radic&radic S000002797SAC7YDR389WverifiedGTPase activating protein (GAP) for Rho1p, involved insignaling to the actin cytoskeleton, nullmutations suppress tor2 mutations andtemperature sensitive mutations in actin;potential Cdc28p substrate 0.000380.01408&radic&radic S000003440SER2YGR208WverifiedPhosphoserine phosphatase of the phosphoglycerate pathway,involved in serine and glycine biosynthesis,expression is regulated by the availablenitrogen source 0.000370.0138&radic S000006219YPR015CUncharacterizedPutative protein of unknown function 0.000370.0135&radic S000001327FIS1YIL065CverifiedMitochondrial outer membrane protein involved in membranefission, required for localization of Dnm1p andMdv1p during mitochondrial division 0.000370.0135&radic&radic&radic S000000421ATG12YBR217WverifiedUbiquitin-like modifier, conjugated via an isopeptide bond to alysine residue of Atg5p by the E1 enzyme,Atg7p, and the E2 enzyme, Atg10p, a step thatis essential for autophagy 0.000370.01337&radic S000005012FKH2YNL068CverifiedForkhead family transcription factor with a major role in theexpression of G2/M phase genes; positivelyregulates transcriptional elongation; negativerole in chromatin silencing at HML and HMR;substrate of the Cdc28p/Clb5p kinase 0.000360.01332&radic&radic S000003110GPI10YGL142CverifiedIntegral membrane protein involved inglycosylphosphatidylinositol (GPI) anchorsynthesis; putative alpha 1,2mannosyltransferase required for addition ofthe third mannose onto the GPI core structure;human PIG-Bp is a functional homolog 0.000360.01317&radic S000002248RAM1YDL090CverifiedBeta subunit of the CAAX farnesyltransferase (FTase) thatprenylates the a-factor mating pheromone andRas proteins; required for the membranelocalization of Ras proteins and a-factor;homolog of the mammalian FTase beta subunit 0.000360.01308&radic S000000601SAT4YCR008WverifiedSer/Thr protein kinase involved in salt tolerance; funtions inregulation of Trk1p-Trk2p potassiumtransporter; partially redundant with Hal5p;has similarity to Npr1p 0.000360.01291&radic&radic S000001001YAP3YHL009CverifiedBasic leucine zipper (bZIP) transcription factor 0.000360.01291&radic&radic S000004677IRC21YMR073CuncharacterizedPutative protein of unknown function; proposed to be involvedin resistance to carboplatin and cisplatin;shares similarity to a human cytochromeoxidoreductase; null mutant displays increasedlevels of spontaneous Rad52 foci