Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0006379 "mRNA cleavage"

GO branch: BP
Number of currently annotated genes: 15

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest TAP:162Kumar:nucleusHuh:nucleusTAP:111Lum:ClomipraminePS50158IPR000504IPR010655Lum:DaunorubicinHarbison:MGA1_YPD S000004267YSH1YLR277CverifiedPutative endonuclease, subunit of the mRNA cleavage andpolyadenylation specificity complex requiredfor 3' processing of mRNAs &radic0.375550.91704&radic&radic&radic&radic&radic S000003388PTI1YGR156WverifiedPta1p Interacting protein &radic0.366330.9106&radic&radic&radic&radic&radic&radic&radic S000004105CFT2YLR115WverifiedSubunit of the mRNA cleavage and polyadenlylation factor (CPF);required for pre-mRNA cleavage, polyadenylationand poly(A) site recognition, 43% similaritywith the mammalian CPSF-100 protein. &radic0.344840.87939&radic&radic&radic&radic&radic&radic S000005261PFS2YNL317WverifiedIntegral subunit of the pre-mRNA cleavage and polyadenylationfactor (CPF) complex; plays an essential rolein mRNA 3'-end formation by bridging differentprocessing factors and thereby promoting theassembly of the processing complex &radic0.336280.87179&radic&radic&radic&radic&radic S000005166SSU72YNL222WverifiedTranscription/RNA-processing factor that associates with TFIIBand cleavage/polyadenylation factor Pta1p;exhibits phosphatase activity on serine-5 ofthe RNA polymerase II C-terminal domain;affects start site selection in vivo 0.335170.87145&radic&radic&radic&radic S000000935GLC7YER133WverifiedCatalytic subunit of type 1 serine/threonine proteinphosphatase, involved in many processesincluding glycogen metabolism, sporulation, andmitosis; interacts with multiple regulatorysubunits; predominantly isolated with Sds22p 0.331830.86805&radic&radic&radic&radic S000000041PTA1YAL043CverifiedSubunit of holo-CPF, a multiprotein complex and functionalhomolog of mammalian CPSF, required for thecleavage and polyadenylation of mRNA and snoRNA3' ends; involved in pre-tRNA processing; bindsto the phosphorylated CTD of RNAPII &radic0.321750.85868&radic&radic&radic&radic&radic&radic S000004665RNA14YMR061WverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; bridges interaction between Rna15p andHrp1p in the CF I complex &radic0.302360.8415&radic&radic&radic S000002636PCF11YDR228CverifiedmRNA 3' end processing factor, essential component of cleavageand polyadenylation factor IA (CF IA), involvedin pre-mRNA 3' end processing and intranscription termination; binds C-terminaldomain of largest subunit of RNA pol II(Rpo21p) &radic0.298410.83995&radic&radic&radic&radic S000003853FIP1YJR093CverifiedSubunit of cleavage polyadenylation factor (CPF), interactsdirectly with poly(A) polymerase (Pap1p) toregulate its activity &radic0.279240.82616&radic&radic&radic S000006311YTH1YPR107CverifiedEssential RNA-binding component of cleavage and polyadenylationfactor, contains five zinc fingers; requiredfor pre-mRNA 3'-end processing andpolyadenylation &radic0.240620.80014&radic&radic&radic&radic&radic&radic S000002709CFT1YDR301WverifiedRNA-binding subunit of the mRNA cleavage and polyadenylationfactor; involved in poly(A) site recognitionand required for both pre-mRNA cleavage andpolyadenylation, 51% sequence similarity withmammalian AAUAA-binding subunit of CPSF &radic0.232890.78814&radic&radic&radic S000003012RNA15YGL044CverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; interacts with the A-rich polyadenylationsignal in complex with Rna14p and Hrp1p &radic0.192040.74914&radic&radic&radic S000005705SYC1YOR179CverifiedSubunit of the APT subcomplex of cleavage and polyadenylationfactor, may have a role in 3' end formation ofboth polyadenylated and non-polyadenylated RNAs 0.181230.7435&radic&radic S000005776CLP1YOR250CverifiedSubunit of cleavage factor I (CFI), involved in both theendonucleolyitc cleavage and polyadenylationsteps of mRNA 3'-end maturation &radic0.151250.7032&radic&radic&radic&radic S000002603REF2YDR195WverifiedRNA-binding protein involved in the cleavage step of mRNA3'-end formation prior to polyadenylation, andin snoRNA maturation; part of holo-CPFsubcomplex APT, which associates with 3'-endsof snoRNA- and mRNA-encoding genes 0.148020.69722&radic&radic&radic&radic S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif &radic0.137840.6851&radic&radic&radic&radic&radic&radic S000001855ACT1YFL039CverifiedActin, structural protein involved in cell polarization,endocytosis, and other cytoskeletal functions 0.112280.64076&radic S000003038RPB9YGL070CverifiedRNA polymerase II subunit B12.6; contacts DNA; mutations affecttranscription start site; involved in telomeremaintenance 0.111850.64022&radic S000000551YCL046WDubiousHypothetical protein 0.100870.61799&radic&radic S000004975HHF2YNL030WverifiedOne of two identical histone H4 proteins (see also HHF1); corehistone required for chromatin assembly andchromosome function; contributes to telomericsilencing; N-terminal domain involved inmaintaining genomic integrity 0.06110.53272&radic&radic&radic S000001297CKA1YIL035CverifiedAlpha catalytic subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA2, CKB1 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 0.059510.52805&radic&radic&radic S000000967PAB1YER165WverifiedPoly(A) binding protein, part of the 3'-end RNA-processingcomplex, mediates interactions between the 5'cap structure and the 3' mRNA poly(A) tail,involved in control of poly(A) tail length,interacts with translation factor eIF-4G 0.05290.50438&radic&radic S000005871TYE7YOR344CverifiedSerine-rich protein that contains a basic-helix-loop-helix(bHLH) DNA binding motif; binds E-boxes ofglycolytic genes and contributes to theiractivation; may function as a transcriptionalactivator in Ty1-mediated gene expression 0.051390.49572&radic&radic&radic S000004198SEC13YLR208WverifiedComponent of both the Nup84 nuclear pore sub-complex and of theCOPII complex (Sar1p, Sec13p, Sec16p, Sec23p,Sec24p, Sec31p, Sfb2p, and Sfb3p) which isimportant for the formation of ER to Golgitransport vesicles 0.045570.4721&radic&radic&radic S000002633HTA1YDR225WverifiedOne of two nearly identical (see also HTA2) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.044970.46738&radic&radic S000000309VID24YBR105CverifiedPeripheral membrane protein located at Vid (vacuole import anddegradation) vesicles; regulatesfructose-1,6-bisphosphatase (FBPase) targetingto the vacuole; involved inproteasome-dependent catabolite degradation ofFBPase 0.044340.46286&radic&radic S000003080TAF6YGL112CverifiedSubunit (60 kDa) of TFIID and SAGA complexes, involved intranscription initiation of RNA polymerase IIand in chromatin modification, similar tohistone H4 0.041440.45134&radic&radic S000000171SSA3YBL075CverifiedATPase involved in protein folding and the response to stress;plays a role in SRP-dependent cotranslationalprotein-membrane targeting and translocation;member of the heat shock protein 70 (HSP70)family; localized to the cytoplasm 0.040970.4488&radic&radic S000004211RSA3YLR221CverifiedProtein with a likely role in ribosomal maturation, requiredfor accumulation of wild-type levels of large(60S) ribosomal subunits; binds to the helicaseDbp6p in pre-60S ribosomal particles in thenucleolus 0.041020.4488&radic S000003017TIF4632YGL049CverifiedTranslation initiation factor eIF4G, subunit of the mRNAcap-binding protein complex (eIF4F) that alsocontains eIF4E (Cdc33p); associates with thepoly(A)-binding protein Pab1p, also interactswith eIF4A (Tif1p); homologous to Tif4631p 0.03890.43842&radic&radic S000004492YML030WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YML030W is not an essential gene 0.038480.43593&radic&radic&radic S000001027VMR1YHL035CverifiedProtein of unknown function that may interact with ribosomes,based on co-purification experiments; member ofthe ATP-binding cassette (ABC) family;potential Cdc28p substrate; detected inpurified mitochondria in high-throughputstudies 0.038460.43593&radic&radic S000003472PFK1YGR240CverifiedAlpha subunit of heterooctameric phosphofructokinase involvedin glycolysis, indispensable for anaerobicgrowth, activated by fructose-2,6-bisphosphateand AMP, mutation inhibits glucose induction ofcell cycle-related genes 0.037440.42928&radic&radic S000002354SEC31YDL195WverifiedEssential phosphoprotein component (p150) of the COPII coat ofsecretory pathway vesicles, in complex withSec13p; required for ER-derived transportvesicle formation 0.036160.42169&radic S000001217ENO2YHR174WverifiedEnolase II, a phosphopyruvate hydratase that catalyzes theconversion of 2-phosphoglycerate tophosphoenolpyruvate during glycolysis and thereverse reaction during gluconeogenesis;expression is induced in response to glucose 0.033630.40781&radic S000002218TSR1YDL060WverifiedProtein required for processing of 20S pre-rRNA in thecytoplasm, associates with pre-40S ribosomalparticles 0.027460.37171&radic S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.027280.36999&radic&radic&radic S000002452RPC11YDR045CverifiedRNA polymerase III subunit C11; mediates pol III RNA cleavageactivity and is important for termination oftranscription 0.02570.35947 S000002430SES1YDR023WverifiedCytosolic seryl-tRNA synthetase, class II aminoacyl-tRNAsynthetase that aminoacylates tRNA(Ser),displays tRNA-dependent amino acid recognitionwhich enhances discrimination of the serinesubstrate, interacts with peroxin Pex21p 0.024970.35433 S000004895AEP2YMR282CverifiedMitochondrial protein, likely involved in translation of themitochondrial OLI1 mRNA; exhibits geneticinteraction with the OLI1 mRNA 5'-untranslatedleader 0.024330.34913&radic S000002789YRA1YDR381WverifiedNuclear protein that binds to RNA and to Mex67p, required forexport of poly(A)+ mRNA from the nucleus;member of the REF (RNA and export factorbinding proteins) family; another familymember, Yra2p, can substitute for Yra1pfunction 0.019540.30476&radic&radic&radic&radic&radic S000002758ATP22YDR350CverifiedMitochondrial inner membrane protein required for assembly ofthe F0 sector of mitochondrial F1F0 ATPsynthase, which is a large, evolutionarilyconserved enzyme complex required for ATPsynthesis 0.017230.27477&radic&radic S000002453BAP3YDR046CverifiedAmino acid permease involved in the uptake of cysteine,leucine, isoleucine and valine 0.014470.23742&radic&radic S000000323MUD1YBR119WverifiedU1 snRNP A protein, homolog of human U1-A; involved in nuclearmRNA splicing 0.014240.23429&radic&radic&radic S000004513RSE1YML049CverifiedProtein involved in pre-mRNA splicing; component of thepre-spliceosome; associates with U2 snRNA;involved in ER to Golgi transport 0.013490.22332 S000001150GGA2YHR108WverifiedGolgi-localized protein with homology to gamma-adaptin,interacts with and regulates Arf1p and Arf2p ina GTP-dependent manner in order to facilitatetraffic through the late Golgi 0.012910.21396&radic S000002349UFD2YDL190CverifiedUbiquitin chain assembly factor (E4) that cooperates with aubiquitin-activating enzyme (E1), aubiquitin-conjugating enzyme (E2), and aubiquitin protein ligase (E3) to conjugateubiquitin to substrates; also functions as an 0.012160.20373&radic S000002697RTT103YDR289CverifiedProtein that interacts with exonuclease Rat1p and Rai1p andplays a role in transcription termination byRNA polymerase II, has an RPR domain(carboxy-terminal domain interacting domain);also involved in regulation of Ty1transposition 0.011230.19124&radic&radic&radic S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.011160.19039&radic&radic S000004642SUB1YMR039CverifiedTranscriptional coactivator, facilitates elongation byinfluencing enzymes that modify RNAP II, actsin a peroxide resistance pathway involvingRad2p; suppressor of TFIIB mutations 0.011160.19039&radic&radic&radic S000005195NRD1YNL251CverifiedRNA-binding protein that interacts with the C-terminal domainof the RNA polymerase II large subunit(Rpo21p), required for transcriptiontermination and 3' end maturation ofnonpolyadenylated RNAs 0.011090.18923&radic&radic&radic&radic&radic S000005483HRP1YOL123WverifiedSubunit of cleavage factor I, a five-subunit complex requiredfor the cleavage and polyadenylation ofpre-mRNA 3' ends; RRM-containing heteronuclearRNA binding protein and hnRNPA/B family memberthat binds to poly (A) signal sequences &radic0.010520.1819&radic&radic&radic S000003566VPS53YJL029CverifiedComponent of the GARP (Golgi-associated retrograde protein)complex, Vps51p-Vps52p-Vps53p-Vps54p, which isrequired for the recycling of proteins fromendosomes to the late Golgi; required forvacuolar protein sorting 0.010320.17879&radic S000003969RNP1YLL046CverifiedRibonucleoprotein that contains two RNA recognition motifs(RRM) 0.010250.17784&radic&radic S000001488BYE1YKL005CverifiedNegative regulator of transcription elongation, contains aTFIIS-like domain and a PHD finger, multicopysuppressor of temperature-sensitive ess1mutations, probably binds RNA polymerase IIlarge subunit 0.009170.16071&radic S000003824RPA12YJR063WverifiedRNA polymerase I subunit A12.2; contains two zinc bindingdomains, and the N terminal domain isresponsible for anchoring to the RNA pol Icomplex 0.009160.16071 S000006337SPN1YPR133CverifiedProtein that interacts with Spt6p and copurifies with Spt5p andRNA polymerase II, probable transcriptionalelongation factor; metazoan homologs contain anacidic N terminus; mutations in the gene conferan Spt- phenotype 0.008730.15392&radic&radic&radic S000001710PAP1YKR002WverifiedPoly(A) polymerase, one of three factors required for mRNA3'-end polyadenylation, forms multiproteincomplex with polyadenylation factor I (PF I),also required for mRNA nuclear export; may alsopolyadenylate rRNAs 0.008480.14978&radic&radic&radic S000004106MSL5YLR116WverifiedComponent of the commitment complex, which defines the firststep in the splicing pathway; essential proteinthat interacts with Mud2p and Prp40p, forming abridge between the intron ends; also involvedin nuclear retention of pre-mRNA 0.008440.14942&radic&radic&radic&radic S000002299RPO21YDL140CverifiedRNA polymerase II largest subunit B220, part of central core;phosphorylation of C-terminal heptapeptiderepeat domain regulates association withtranscription and splicing factors; similar tobacterial beta-prime 0.008210.14609&radic&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p 0.007980.14265&radic&radic&radic S000005566GLO4YOR040WverifiedMitochondrial glyoxalase II, catalyzes the hydrolysis ofS-D-lactoylglutathione into glutathione andD-lactate 0.007750.13904&radic&radic S000005677RPB2YOR151CverifiedRNA polymerase II second largest subunit B150, part of centralcore; similar to bacterial beta subunit 0.007380.13276&radic&radic&radic S000003959PRP19YLL036CverifiedSplicing factor associated with the spliceosome; contains aU-box, a motif found in a class of ubiquitinligases 0.007230.12997&radic&radic S000002680GLO2YDR272WverifiedCytoplasmic glyoxalase II, catalyzes the hydrolysis ofS-D-lactoylglutathione into glutathione andD-lactate 0.007180.12906&radic&radic&radic S000005816SNF2YOR290CverifiedCatalytic subunit of the SWI/SNF chromatin remodeling complexinvolved in transcriptional regulation;contains DNA-stimulated ATPase activity;functions interdependently in transcriptionalactivation with Snf5p and Snf6p 0.007130.12869&radic&radic S000005199GIS2YNL255CverifiedProtein with seven cysteine-rich CCHC zinc-finger motifs,similar to human CNBP, proposed to be involvedin the RAS/cAMP signaling pathway 0.006890.1244&radic&radic S000003279TFC4YGR047CverifiedOne of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauA domain of TFIIIC that binds BoxA DNApromoter sites of tRNA and similar genes; hasTPR motifs; human homolog is TFIIIC-102 0.006820.12322&radic&radic&radic S000002495SLU7YDR088CverifiedRNA splicing factor, required for ATP-independent portion of2nd catalytic step of spliceosomal RNAsplicing; interacts with Prp18p; contains zincknuckle domain 0.006820.12322&radic&radic&radic&radic&radic S000005524BDS1YOL164WverifiedBacterially-derived sulfatase required for use of alkyl- andaryl-sulfates as sulfur sources 0.006590.1195 S000000939YER137CUncharacterizedHypothetical protein 0.006410.11645 S000004668AEP1YMR064WverifiedProtein required for expression of the mitochondrial OLI1 geneencoding subunit 9 of F1-F0 ATP synthase 0.00640.11617 S000003484GCN5YGR252WverifiedHistone acetyltransferase, acetylates N-terminal lysines onhistones H2B and H3; catalytic subunit of theADA and SAGA histone acetyltransferasecomplexes; founding member of the Gcn5p-relatedN-acetyltransferase superfamily 0.005930.10776&radic&radic S000002334AIR2YDL175CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p; may regulatemethylation of Npl3p, which modulates Npl3pfunction in mRNA processing and export; hassimilarity to Air1p 0.005930.10765&radic&radic&radic S000003704SET2YJL168CverifiedHistone methyltransferase with a role in transcriptionalelongation, methylates a lysine residue ofhistone H3; associates with the C-terminaldomain of Rpo21p; histone methylation activityis regulated by phosphorylation status ofRpo21p 0.005690.10215&radic&radic&radic S000001022ECM29YHL030WverifiedMajor component of the proteasome; tethers the proteasome coreparticle to the regulatory particle, andenhances the stability of the proteasome 0.005610.1005&radic&radic&radic&radic S000000285SPT7YBR081CverifiedSubunit of the SAGA transcriptional regulatory complex,involved in proper assembly of the complex;also present as a C-terminally truncated formin the SLIK/SALSA transcriptional regulatorycomplex 0.005380.09618&radic&radic S000003391NSR1YGR159CverifiedNucleolar protein that binds nuclear localization sequences,required for pre-rRNA processing and ribosomebiogenesis 0.00490.08701&radic&radic&radic&radic S000005119NOP13YNL175CverifiedProtein of unknown function, localizes to the nucleolus andnucleoplasm; contains an RNA recognition motif(RRM) and has similarity to Nop12p, which isrequired for processing of pre-18S rRNA 0.004840.08591&radic&radic&radic&radic&radic S000001448MSL1YIR009WverifiedU2B component of U2 snRNP, involved in splicing, binds the U2snRNA stem-loop IV in vitro; does not containthe conserved C-terminal RNA binding domainfound in other family members 0.004860.08591&radic&radic S000001026SBP1YHL034CverifiedNucleolar single-strand nucleic acid binding protein;associates with small nuclear RNAs 0.004840.08591&radic&radic&radic&radic S000003329ASK10YGR097WverifiedComponent of the RNA polymerase II holoenzyme, phosphorylatedin response to oxidative stress; has a role indestruction of Ssn8p, which relieves repressionof stress-response genes 0.004770.08405&radic&radic S000004315CWC24YLR323CverifiedEssential protein, component of a complex containing Cef1p; hassimilarity to S. pombe Cwf24p 0.004720.08347&radic&radic&radic S000005401NOP12YOL041CverifiedNucleolar protein, required for pre-25S rRNA processing;contains an RNA recognition motif (RRM) and hassimilarity to Nop13p, Nsr1p, and putativeorthologs in Drosophila and S. pombe 0.004670.08283&radic&radic&radic S000000221KAP104YBR017CverifiedTransportin, cytosolic karyopherin beta 2 involved in deliveryof heterogeneous nuclear ribonucleoproteins tothe nucleoplasm, binds rg-nuclear localizationsignals on Nab2p and Hrp1p, plays a role incell-cycle progression 0.004640.08228&radic S000003506TAF1YGR274CverifiedTFIID subunit (145 kDa), involved in RNA polymerase IItranscription initiation, has histoneacetyltransferase activity, involved inpromoter binding and G1/S progression 0.004630.0819&radic&radic&radic&radic S000005054NOP15YNL110CverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis; localizes to bothnucleolus and cytoplasm 0.004610.08177&radic&radic&radic S000006111NAB3YPL190CverifiedSingle stranded DNA binding protein; acidic ribonucleoprotein;required for termination of non-poly(A)transcripts and efficient splicing; interactswith Nrd1p 0.004610.08172&radic&radic&radic S000001622CTK1YKL139WverifiedCatalytic (alpha) subunit of C-terminal domain kinase I(CTDK-I), which phosphorylates the C-terminalrepeated domain of the RNA polymerase II largesubunit (Rpo21p) to affect both transcriptionand pre-mRNA 3' end processing 0.004580.08104&radic&radic S000004126TIS11YLR136CverifiedmRNA-binding protein expressed during iron starvation; binds toa sequence element in the 3'-untranslatedregions of specific mRNAs to mediate theirdegradation; involved in iron homeostasis 0.004490.07942&radic&radic S000001557MUD2YKL074CverifiedProtein involved in early pre-mRNA splicing; component of thepre-mRNA-U1 snRNP complex, the commitmentcomplex; interacts with Msl5p/BBP splicingfactor and Sub2p; similar to metazoan splicingfactor U2AF65 0.004470.07894&radic&radic&radic&radic&radic S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.004480.07894 S000001323SNP1YIL061CverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; may interact with poly(A)polymerase to regulate polyadenylation; homologof human U1 70K protein 0.004310.0757&radic&radic&radic S000002383WHI4YDL224CverifiedPutative RNA binding protein and partially redundant Whi3phomolog that regulates the cell sizerequirement for passage through Start andcommitment to cell division 0.004270.07492&radic&radic S000004903HAS1YMR290CverifiedATP-dependent RNA helicase; localizes to both the nuclearperiphery and nucleolus; highly enriched innuclear pore complex fractions; constituent of66S pre-ribosomal particles 0.004270.07492&radic&radic&radic&radic S000005076KRE33YNL132WverifiedEssential protein of unknown function; heterozygous mutantshows haploinsufficiency in K1 killer toxinresistance 0.004230.07393&radic S000004961PUB1YNL016WverifiedPoly(A)+ RNA-binding protein, abundant mRNP-component proteinthat binds mRNA and is required for stabilityof a number of mRNAs; not reported to associatewith polyribosomes 0.004120.07191&radic&radic S000004401UTP21YLR409CuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.004110.07147&radic&radic S000001697YRA2YKL214CverifiedMember of the REF (RNA and export factor binding proteins)family; when overexpressed, can substitute forthe function of Yra1p in export of poly(A)+mRNA from the nucleus 0.004080.07102&radic&radic&radic S000003893NMD5YJR132WverifiedKaryopherin, a carrier protein involved in nuclear import ofproteins; importin beta homolog 0.004070.07102&radic S000001919PES4YFR023WverifiedPoly(A) binding protein, suppressor of DNA polymerase epsilonmutation, similar to Mip6p 0.004060.07023&radic&radic&radic S000003796RAD26YJR035WverifiedProtein involved in transcription-coupled repair nucleotideexcision repair of UV-induced DNA lesions;homolog of human CSB protein 0.003890.06684&radic S000003295SPT4YGR063CverifiedProtein that forms a complex with Spt5p and mediates bothactivation and inhibition of transcriptionelongation, and plays a role in pre-mRNAprocessing; in addition, Spt4p is involved inkinetochore function and gene silencing 0.003840.06568&radic S000001450STS1YIR011CverifiedProtein that interacts with the karyopherin Srp1p; may have arole with Srp1p in ubiquitin-mediated proteindegradation 0.003830.06568&radic&radic S000002857UTP6YDR449CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.003830.06528&radic S000001440SGN1YIR001CverifiedCytoplasmic RNA-binding protein, contains an RNA recognitionmotif (RRM); may have a role in mRNAtranslation, as suggested by geneticinteractions with genes encoding proteinsinvolved in translational initiation 0.00380.06498&radic&radic S000000449ISW1YBR245CverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPase thatforms a complex with Ioc2p and Ioc4p toregulate transcription elongation, and acomplex with Ioc3p to repress transcriptioninitiation 0.003810.06498&radic&radic&radic S000005846HSH49YOR319WverifiedU2-snRNP associated splicing factor with similarity to themammalian splicing factor SAP49; proposed tofunction as a U2-snRNP assembly factor alongwith Hsh155p and binding partner Cus1p;contains two RNA recognition motifs (RRM) 0.003770.06405&radic&radic&radic&radic&radic S000003237TFG2YGR005CverifiedTFIIF (Transcription Factor II) middle subunit; involved inboth transcription initiation and elongation ofRNA polymerase II; homologous to human RAP30 0.003710.06303&radic S000005337ESF2YNR054CverifiedEssential nucleolar protein involved in pre-18S rRNAprocessing; component of the small subunit(SSU) processome; has sequence similarity tomABT1, a mouse transcription activator 0.00370.06287&radic&radic S000002851SSN2YDR443CverifiedProtein required for stable association of Srb10p-Srb11p kinasewith RNA polymerase holoenzyme; subunit of theRNA polymerase II mediator complex; essentialfor transcriptional regulation 0.003680.06252&radic S000002432RPS11AYDR025WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps11Bp and has similarity to E.coli S17 and rat S11 ribosomal proteins 0.003630.06143&radic S000004825EFR3YMR212CverifiedNon-essential protein of unknown function; exhibits syntheticlethal genetic interactions with PHO85; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 0.003460.05833 S000006099CBC2YPL178WverifiedSmall subunit of the heterodimeric cap binding complex thatalso contains Sto1p, component of thespliceosomal commitment complex; interacts withNpl3p, possibly to package mRNA for export fromthe nucleus; contains an RNA-binding motif 0.003440.05808&radic&radic&radic&radic&radic S000000539LSB5YCL034WverifiedProtein of unknown function; binds Las17p, which is a homologof human Wiskott-Aldrich Syndrome proteininvolved in actin patch assembly and actinpolymerization 0.003380.05719&radic&radic S000002558CTH1YDR151CverifiedMember of the CCCH zinc finger family; has similarity tomammalian Tis11 protein, which activatestranscription and also has a role in mRNAdegradation; may function with Tis11p in ironhomeostasis 0.003380.05705&radic S000004949HRB1YNL004WverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toGbp2p and Npl3p 0.003360.05673&radic&radic S000003427SKI6YGR195Wverified3'-to-5' phosphorolytic exoribonuclease that is a subunit ofthe exosome; required for 3' processing of the5.8S rRNA; involved in 3' to 5' mRNAdegradation and translation inhibition ofnon-poly(A) mRNAs 0.003350.0565&radic&radic&radic S000005230CUS2YNL286WverifiedProtein that binds to U2 snRNA and Prp11p, may be involved inU2 snRNA folding; contains two RNA recognitionmotifs (RRMs) 0.003240.05498&radic&radic S000005289VPS27YNR006WverifiedEndosomal protein that forms a complex with Hse1p; required forrecycling Golgi proteins, forming lumenalmembranes and sorting ubiquitinated proteinsdestined for degradation; has UbiquitinInteraction Motifs which bind ubiquitin (Ubi4p) 0.003220.05462 S000002766GGA1YDR358WverifiedGolgi-localized protein with homology to gamma-adaptin,interacts with and regulates Arf1p and Arf2p ina GTP-dependent manner in order to facilitatetraffic through the late Golgi 0.00320.05395&radic S000000416NGR1YBR212WverifiedRNA binding protein that negatively regulates growth rate;interacts with the 3' UTR of the mitochondrialporin (POR1) mRNA and enhances its degradation;overexpression impairs mitochondrial function;expressed in stationary phase 0.003190.05395&radic&radic S000005141WHI3YNL197CverifiedRNA binding protein that sequesters CLN3 mRNA in cytoplasmicfoci; cytoplasmic retention factor for Cdc28pand associated cyclins; regulates cell fate anddose-dependently regulates the critical cellsize required for passage through Start 0.003150.05306&radic&radic S000005975LEE1YPL054WverifiedZinc-finger protein of unknown function 0.003150.05306&radic S000002560ENT5YDR153CverifiedProtein containing an N-terminal epsin-like domain involved inclathrin recruitment and traffic between theGolgi and endosomes; associates with theclathrin adaptor Gga2p, clathrin adaptorcomplex AP-1, and clathrin 0.003130.05306&radic S000006246PUF2YPR042CverifiedMember of the PUF protein family, which is defined by thepresence of Pumilio homology domains thatconfer RNA binding activity; preferentiallybinds mRNAs encoding membrane-associatedproteins 0.003140.05306&radic S000006132NIP7YPL211WverifiedNucleolar protein required for 60S ribosome subunit biogenesis,constituent of 66S pre-ribosomal particles;physically interacts with Nop8p and the exosomesubunit Rrp43p 0.003150.05306&radic&radic&radic S000005963SSN3YPL042CverifiedCyclin-dependent protein kinase, component of RNA polymerase IIholoenzyme; involved in phosphorylation of theRNA polymerase II C-terminal domain; involvedin glucose repression 0.00310.05269&radic S000003026RAD6YGL058WverifiedUbiquitin-conjugating enzyme (E2), involved in postreplicationrepair (with Rad18p), sporulation, telomeresilencing, and ubiquitin-mediated N-end ruleprotein degradation (with Ubr1p) 0.003090.05256&radic&radic&radic&radic S000002837TIF35YDR429CverifiedSubunit of the core complex of translation initiation factor3(eIF3), which is essential for translation 0.003070.05211&radic&radic S000005617TMA46YOR091WverifiedProtein of unknown function that associates with ribosomes;interacts with GTPase Rbg1p 0.003030.05162 S000000517GBP2YCL011CverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toHrb1p and Npl3p; also binds single-strandedtelomeric repeat sequence in vitro 0.003030.05162&radic&radic&radic&radic S000005504NOP8YOL144WverifiedNucleolar protein required for 60S ribosomal subunit biogenesis 0.002990.051&radic S000003482YGR250CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.002920.05002&radic S000004393DUS3YLR401CuncharacterizedDihydrouridine synthase, member of a widespread family ofconserved proteins including Smm1p, Dus1p, andDus4p; contains a consensus oleate responseelement (ORE) in its promoter region 0.002930.05002&radic S000006316MRD1YPR112CverifiedEssential conserved protein that associates with 35S precursorrRNA and is required for its initial processingat the A(0)-A(2) cleavage sites, shows partialnucleolar localization, contains five consensusRNA-binding domains 0.00290.04968&radic&radic S000005964NOP4YPL043WverifiedNucleolar protein, essential for processing and maturation of27S pre-rRNA and large ribosomal subunitbiogenesis; constituent of 66S pre-ribosomalparticles; contains four RNA recognition motifs(RRMs) 0.002890.04952&radic&radic&radic S000004470SPT5YML010WverifiedProtein that forms a complex with Spt4p and mediates bothactivation and inhibition of transcriptionelongation; Spt4p-Spt5p complex also plays arole in pre-mRNA processing 0.002890.04952&radic&radic S000000870MOT2YER068WverifiedComponent of the CCR4-NOT complex, which has multiple roles intranscription regulation, mRNA degradation, andpost-transcriptional modifications; with Ubc4p,ubiquitylates subunits of the nascentpolypeptide-associated complex (NAC) 0.002880.04938&radic&radic S000004685NAM7YMR080CverifiedATP-dependent RNA helicase of the SFI superfamily, required fornonsense mediated mRNA decay and for efficienttranslation termination at nonsense codons;involved in telomere maintenance 0.002830.04864 S000001537DEF1YKL054CverifiedRNAPII degradation factor, forms a complex with Rad26p inchromatin, enables ubiquitination andproteolysis of RNAPII present in an elongationcomplex 0.002810.04843&radic&radic S000002368CWC2YDL209CverifiedProtein involved in pre-mRNA splicing, component of a complexcontaining Cef1p; interacts with Prp19p;contains an RNA recognition motif; hassimilarity to S. pombe Cwf2p 0.00280.04821&radic&radic S000002326NRP1YDL167CverifiedProtein of unknown function, rich in asparagine residues 0.00280.0482&radic S000004917YME2YMR302CverifiedIntegral inner mitochondrial membrane protein with similarityto exonucleases; mutants exhibit an increasedrate of mitochondrial DNA escape 0.002780.04779&radic S000005888PRT1YOR361CverifiedSubunit of the core complex of translation initiation factor3(eIF3), essential for translation; part of asubcomplex (Prt1p-Rpg1p-Nip1p) that stimulatesbinding of mRNA and tRNA(i)Met to ribosomes 0.002760.04763&radic&radic&radic S000001444IST3YIR005WverifiedComponent of the U2 snRNP, required for the first catalyticstep of splicing and for spliceosomal assembly;interacts with Rds3p and is required forMer1p-activated splicing 0.002760.04763&radic&radic S000005334BRE5YNR051CverifiedUbiquitin protease cofactor, forms deubiquitination complexwith Ubp3p that coregulates anterograde andretrograde transport between the endoplasmicreticulum and Golgi compartments; null issensitive to brefeldin A 0.002730.04697&radic S000000973RAD3YER171Wverified5' to 3' DNA helicase, involved in nucleotide excision repairand transcription; subunit of RNA polymerase IItranscription initiation factor TFIIH; subunitof Nucleotide Excision Repair Factor 3 (NEF3);homolog of human XPD protein 0.002690.04657&radic S000000147PIN4YBL051CverifiedProtein involved in G2/M phase progression and response to DNAdamage, interacts with Rad53p; contains an RNArecognition motif, a nuclear localizationsignal, and several SQ/TQ cluster domains;hyperphosphorylated in response to DNA damage 0.002650.04614&radic&radic S000003851JSN1YJR091CverifiedMember of the Puf family of RNA-binding proteins, interactswith mRNAs encoding membrane-associatedproteins; overexpression suppresses a tub2-150mutation and causes increased sensitivity tobenomyl in wild-type cells 0.002640.04604&radic S000006161HSP82YPL240CverifiedCytoplasmic chaperone (Hsp90 family) required for pheromonesignaling and negative regulation of Hsf1p;docks with the mitochondrial import receptorTom70p for preprotein delivery; interacts withco-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p 0.00260.04544&radic&radic S000006105MRN1YPL184CuncharacterizedPutative RNA binding protein 0.002610.04544&radic&radic S000003192SDT1YGL224CverifiedPyrimidine nucleotidase; overexpression suppresses the 6-AUsensitivity of transcription elongation factorS-II, as well as resistance to other pyrimidinederivatives 0.002610.04544 S000003570HCA4YJL033WverifiedPutative nucleolar DEAD box RNA helicase; high-copy numbersuppression of a U14 snoRNA processing mutantsuggests an involvement in 18S rRNA synthesis 0.002610.04544&radic S000000638TAF2YCR042CverifiedTFIID subunit (150 kDa), involved in RNA polymerase IItranscription initiation 0.002560.04497&radic S000002372NOP6YDL213CuncharacterizedProtein with similarity to hydrophilins, which are involved inthe adaptive response to hyperosmoticconditions; computational analysis oflarge-scale protein-protein interaction datasuggests a possible role in rRNA processing 0.002540.04463&radic&radic S000006050TAF14YPL129WverifiedSubunit (30 kDa) of TFIID, TFIIF, and SWI/SNF complexes,involved in RNA polymerase II transcriptioninitiation and in chromatin modification,contains a YEATS domain 0.002550.04463&radic&radic&radic S000006367TIF3YPR163CverifiedTranslation initiation factor eIF-4B, has RNA annealingactivity; contains an RNA recognition motif andbinds to single-stranded RNA 0.002560.04463&radic&radic S000003213RTF1YGL244WverifiedSubunit of the RNA polymerase II-associated Paf1 complex;directly or indirectly regulates DNA-bindingproperties of Spt15p and relative activities ofdifferent TATA elements; involved in telomeremaintenance 0.002540.04463&radic&radic&radic&radic S000001292SSM4YIL030CverifiedUbiquitin-protein ligase of the ER/nuclear envelope, requiredfor degradation of Alpha2p and other proteinscontaining a Deg1 degradation signal; ssm4mutation suppresses mRNA instability caused byan rna14 mutation 0.002540.04463 S000004527MFT1YML062CverifiedSubunit of the THO complex, which is a nuclear complexcomprised of Hpr1p, Mft1p, Rlr1p, and Thp2p,that is involved in transcription elongationand mitotic recombination; involved in telomeremaintenance 0.002540.04458&radic S000002209LHP1YDL051WverifiedRNA binding protein required for maturation of tRNA and snRNAprecursors; acts as a molecular chaperone forRNAs transcribed by polymerase III; homologousto human La (SS-B) autoantigen 0.002530.04439&radic&radic S000004881PRP24YMR268CverifiedSplicing factor that reanneals U4 and U6 snRNPs duringspliceosome recycling 0.002520.04422&radic&radic&radic&radic S000004494RAD52YML032CverifiedProtein that stimulates strand exchange by facilitating Rad51pbinding to single-stranded DNA; annealscomplementary single-stranded DNA; involved inthe repair of double-strand breaks in DNAduring vegetative growth and meiosis 0.002490.04386&radic&radic S000001128NAM8YHR086WverifiedRNA binding protein, component of the U1 snRNP protein; mutantsare defective in meiotic recombination and information of viable spores, involved in theformation of DSBs through meiosis-specificsplicing of MER2 pre-mRNA 0.002430.04313&radic&radic&radic S000002545HPR1YDR138WverifiedSubunit of THO/TREX complexes that couple transcriptionelongation with mitotic recombination and withmRNA metabolism and export, subunit of an RNAPol II complex; regulates lifespan; involved intelomere maintenance; similar to Top1p 0.002430.04304&radic&radic S000000064SWD1YAR003WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres; WD40beta propeller superfamily member withsimilarity to mammalian Rbbp7 0.002420.04281&radic S000001057MIP6YHR015WverifiedPutative RNA-binding protein, interacts with Mex67p, which is acomponent of the nuclear pore involved innuclear mRNA export 0.002370.04208&radic&radic&radic S000001016RIM4YHL024WverifiedPutative RNA-binding protein required for the expression ofearly and middle sporulation genes 0.002370.04208&radic S000004075ARP6YLR085CverifiedActin-related protein that binds nucleosomes; a component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.002380.04208&radic&radic S000004585NAB6YML117WuncharacterizedPutative RNA-binding protein, based on computational analysisof large-scale protein-protein interaction data 0.002370.04208&radic S000002742SWR1YDR334WverifiedSwi2/Snf2-related ATPase that is the structural component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.002340.04151&radic&radic S000005929CHL1YPL008WverifiedConserved nuclear protein required to establishsister-chromatid pairing during S-phase,probable DNA helicase with similarity to humanBACH1, which associates with tumor suppressorBRCA1; associates with acetyltransferase Ctf7p 0.002340.04151&radic&radic&radic S000005366TOP1YOL006CverifiedTopoisomerase I, nuclear enzyme that relieves torsional strainin DNA by cleaving and re-sealing thephosphodiester backbone; relaxes bothpositively and negatively supercoiled DNA;functions in replication, transcription, andrecombination 0.00230.04077&radic&radic&radic S000005372HTZ1YOL012CverifiedHistone variant H2AZ, exchanged for histone H2A in nucleosomesby the SWR1 complex; involved intranscriptional regulation through preventionof the spread of silent heterochromatin 0.002290.04064&radic&radic&radic S000005976LGE1YPL055CverifiedProtein of unknown function; null mutant forms abnormally largecells 0.002260.04025&radic S000003418TFG1YGR186WverifiedTFIIF (Transcription Factor II) largest subunit; involved inboth transcription initiation and elongation ofRNA polymerase II; homologous to human RAP74 0.002260.04011&radic&radic S000001928YFR032CUncharacterizedPutative protein of unknown function; transcribed during sporulation; YFR032C is not an essential gene 0.002160.03861&radic S000005365RPB11YOL005CverifiedRNA polymerase II subunit B12.5; part of central core; similarto Rpc19p and bacterial alpha subunit 0.002140.0384&radic&radic&radic S000000168RPS8AYBL072CverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps8Bp and has similarity to ratS8 ribosomal protein 0.002120.03813&radic&radic S000003336SRB5YGR104CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation; involved intelomere maintenance 0.00210.0378&radic&radic S000003450CRM1YGR218WverifiedMajor karyopherin, involved in export of proteins, RNAs, andribosomal subunits from the nucleus 0.002110.0378&radic&radic&radic S000000483PAF1YBR279WverifiedRNA polymerase II-associated protein, defines a large complexthat is biochemically and functionally distinctfrom the Srb-Mediator form of Pol II holoenzymeand is required for full expression of a subsetof cell cycle-regulated genes 0.002070.0374&radic S000004650NUP116YMR047CverifiedSubunit of the nuclear pore complex (NPC) that is localized toboth sides of the pore; contains a repetitiveGLFG motif that interacts with mRNA exportfactor Mex67p and with karyopherin Kap95p;homologous to Nup100p 0.002050.03696&radic&radic S000004505VPS71YML041Cverifiednucleosome-binding component of the SWR1 complex, whichexchanges histone variant H2AZ (Htz1p) forchromatin-bound histone H2A; required forvacuolar protein sorting 0.002030.03666&radic&radic S000000950SPT15YER148WverifiedTATA-binding protein, general transcription factor thatinteracts with other factors to form thepreinitiation complex at promoters, essentialfor viability 0.002030.03666&radic S000004140STM1YLR150WverifiedProtein that binds G4 quadruplex and purine motif triplexnucleic acid; acts with Cdc13p to maintaintelomere structure; interacts with ribosomesand subtelomeric Y' DNA; multicopy suppressorof tom1 and pop2 mutations 0.0020.03607&radic&radic S000003034SGF73YGL066Wverified73 kDa subunit of SAGA histone acetyltransferase complex;involved in formation of the preinitiationcomplex assembly at promoters 0.0020.03607&radic&radic&radic S000003639GSM1YJL103CuncharacterizedPutative zinc cluster protein of unknown function; proposed tobe involved in the regulation of energymetabolism, based on patterns of expression andsequence analysis 0.001990.03607&radic S000006365SGV1YPR161CverifiedCyclin (Bur2p)-dependent protein kinase that functions intranscriptional regulation; phosphorylates thecarboxy-terminal domain of Rpo21p, which is thelargest subunit of RNA polymerase II; regulatedby Cak1p 0.001980.03584&radic&radic S000006348NOC4YPR144CverifiedNucleolar protein, forms a complex with Nop14p that mediatesmaturation and nuclear export of 40S ribosomalsubunits 0.001980.03584&radic&radic&radic&radic S000004424IMD3YLR432WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.001970.03581 S000001501SWD2YKL018WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lys 4 and is involved intelomeric silencing; subunit of CPF (cleavageand polyadenylation factor), a complex involvedin RNAP II transcription termination 0.001960.03553&radic S000001259IMD2YHR216WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, expression is induced bymycophenolic acid resulting in resistance tothe drug, expression is repressed by nutrientlimitation 0.001960.03553 S000000172ILS1YBL076CverifiedCytoplasmic isoleucine-tRNA synthetase, target of theG1-specific inhibitor reveromycin A 0.001920.03501&radic&radic S000004107CLF1YLR117CverifiedEssential splicesome assembly factor; contains multipletetratricopeptide repeat (TPR) protein-bindingmotifs and interacts specifically with manyspliceosome components, may serve as a scaffoldduring splicesome assembly 0.001920.03492&radic&radic S000000160PRX1YBL064CverifiedMitochondrial peroxiredoxin (1-Cys Prx) with thioredoxinperoxidase activity, has a role in reduction ofhydroperoxides; induced during respiratorygrowth and under conditions of oxidative stress 0.001920.03492&radic&radic&radic S000002596CCT6YDR188WverifiedSubunit of the cytosolic chaperonin Cct ring complex, relatedto Tcp1p, essential protein that is requiredfor the assembly of actin and tubulins in vivo;contains an ATP-binding motif 0.001910.03479&radic&radic S000004085IOC2YLR095CverifiedMember of a complex (Isw1b) with Isw1p and Ioc4p that exhibitsnucleosome-stimulated ATPase activity and actswithin coding regions to coordinatetranscription elongation with termination andprocessing, contains a PHD finger motif 0.00190.0346&radic&radic&radic&radic S000004216BUR2YLR226WverifiedCyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2pcomprise a CDK-cyclin complex involved intranscriptional regulation through itsphosphorylation of the carboxy-terminal domainof the largest subunit of RNA polymerase II 0.00190.0346&radic&radic S000004440RPL6BYLR448WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to Rpl6Bp and to rat L6 ribosomalprotein; binds to 5.8S rRNA 0.001870.03403 S000000009SWC3YAL011WverifiedProtein of unknown function, component of the SWR1 complex,which exchanges histone variant H2AZ (Htz1p)for chromatin-bound histone H2A; required forformation of nuclear-associated array of smoothendoplasmic reticulum known as karmellae 0.001840.03324&radic&radic&radic S000003783LSM8YJR022WverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)that is part of spliceosomal U6 snRNP and isalso implicated in processing of pre-tRNA,pre-snoRNA, and pre-rRNA 0.001820.03306&radic&radic S000004641CCS1YMR038CverifiedCopper chaperone for superoxide dismutase Sod1p, involved inoxidative stress protection; Met-X-Cys-X2-Cysmotif within the N-terminal portion is involvedin insertion of copper into Sod1p underconditions of copper deprivation 0.00180.03277&radic&radic&radic S000002824SYF1YDR416WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression; similar to Drosophila crooked neckprotein 0.00180.03267&radic S000002232BRE1YDL074CverifiedE3 ubiquitin ligase for Rad6p, required for the ubiquitinationof histone H2B, recruitment of Rad6p topromoter chromatin and subsequent methylationof histone H3 (on L4 and L79), contains RINGfinger domain 0.001790.03229&radic S000003095SOH1YGL127CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; involved in telomeremaintenance; conserved with other metazoanMED31 subunits 0.001780.03229&radic S000002719TFB1YDR311WverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, required for nucleotide excisionrepair, target for transcriptional activators 0.001790.03229&radic&radic&radic S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus 0.001780.03229&radic&radic S000000379SWD3YBR175WverifiedEssential subunit of the COMPASS (Set1C) complex, whichmethylates histone H3 on lysine 4 and isrequired in transcriptional silencing neartelomeres; WD40 beta propeller superfamilymember and ortholog of mammalian WDR5 0.001770.03204&radic&radic S000002893VPS72YDR485CverifiedHtz1p-binding component of the SWR1 complex, which exchangeshistone variant H2AZ (Htz1p) forchromatin-bound histone H2A; required forvacuolar protein sorting 0.001780.03204&radic S000004509PRP39YML046WverifiedU1 snRNP protein involved in splicing, contains multipletetriatricopeptide repeats 0.001760.03186&radic S000006141RPL1AYPL220WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl1Bpand has similarity to E. coli L1 and rat L10aribosomal proteins; rpl1a rpl1b double nullmutation is lethal 0.001750.03169&radic&radic S000003676RPB4YJL140WverifiedRNA polymerase II subunit B32; forms two subunit dissociablecomplex with Rpb7p; dispensable under someenvironmental conditions; involved in export ofmRNA to cytoplasm under stress conditions;involved in telomere maintenance 0.001750.03155&radic S000003098CEG1YGL130WverifiedAlpha (guanylyltransferase) subunit of the mRNA capping enzyme,a heterodimer (the other subunit is CET1, anRNA 5'-triphophatase) involved in adding the 5'cap to mRNA; the mammalian enzyme is a singlebifunctional polypeptide 0.001750.03155&radic S000000435SWC5YBR231CuncharacterizedProtein of unknown function, component of the SWR1 complex,which exchanges histone variant H2AZ (Htz1p)for chromatin-bound histone H2A 0.001740.03125&radic&radic S000001406TID3YIL144WverifiedComponent of the evolutionarily conservedkinetochore-associated Ndc80 complex(Ndc80p-Nuf2p-Spc24p-Spc25p); conservedcoiled-coil protein involved in chromosomesegregation, spindle checkpoint activity,kinetochore assembly and clustering 0.00170.0305&radic S000000791SIT1YEL065WverifiedFerrioxamine B transporter, member of the ARN family oftransporters that specifically recognizesiderophore-iron chelates; transcription isinduced during iron deprivation and diauxicshift; potentially phosphorylated by Cdc28p 0.001680.03002&radic S000004794RGM1YMR182CverifiedPutative transcriptional repressor with proline-rich zincfingers; overproduction impairs cell growth 0.001680.02976&radic&radic&radic&radic S000000781POL5YEL055CverifiedProtein with sequence similarity to the human MybBP1A and weaksequence similar to B-type DNA polymerases, notrequired for chromosomal DNA replication;required for the synthesis of rRNA 0.001670.02955&radic&radic S000004410CDC73YLR418CverifiedConstituent of Paf1 complex with RNA polymerase II, Paf1p,Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinctfrom Srb-containing Pol II complexes; requiredfor expression of certain genes, modificationof some histones, and telomere maintenance 0.001660.02924&radic S000004296STT4YLR305CverifiedPhosphatidylinositol-4-kinase that functions in the Pkc1pprotein kinase pathway; required for normalvacuole morphology, cell wall integrity, andactin cytoskeleton organization 0.001650.029&radic&radic S000003608PSF2YJL072CverifiedSubunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), whichis localized to DNA replication origins andimplicated in assembly of the DNA replicationmachinery 0.001630.02838 S000001123LRP1YHR081WverifiedSubstrate-specific nuclear cofactor for exosome activity in theprocessing of stable RNAs; required fortelomere length maintenance; homolog ofmammalian nuclear matrix protein C1D involvedin regulation of DNA repair and recombination 0.001640.02838&radic S000000452HIS7YBR248CverifiedImidazole glycerol phosphate synthase (glutamineamidotransferase:cyclase), catalyzes the fifthand sixth steps of histidine biosynthesis andalso produces 5-aminoimidazole-4-carboxamideribotide (AICAR), a purine precursor 0.001610.02739&radic&radic&radic S000002412MAF1YDR005CverifiedNegative regulator of RNA polymerase III; component of severalsignaling pathways that repress polymerase IIItranscription in response to changes incellular environment; targets the initiationfactor TFIIIB 0.001620.02739&radic&radic S000005711GSP2YOR185CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; interacts withKap121p, Kap123p and Pdr6p (karyophilin betas);Gsp1p homolog that is not required forviability 0.001610.02739&radic&radic S000001331RPS24BYIL069CverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps24Ap and has similarity to ratS24 ribosomal protein 0.001610.02707 S000004332RPP0YLR340WverifiedConserved ribosomal protein P0 similar to rat P0, human P0, andE. coli L10e; shown to be phosphorylated onserine 302 0.00160.02668&radic S000005768SSP2YOR242CverifiedSporulation specific protein that localizes to the spore wall;required for sporulation at a point aftermeiosis II and during spore wall formation;SSP2 expression is induced midway in meiosis 0.001590.0261&radic&radic S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.001590.0261&radic&radic S000004034PDC1YLR044CverifiedMajor of three pyruvate decarboxylase isozymes, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde; subject to glucose-, ethanol-,and autoregulation; involved in amino acidcatabolism 0.001580.02591&radic&radic&radic S000000953UBP3YER151CverifiedUbiquitin-specific protease that interacts with Bre5p toco-regulate anterograde and retrogradetransport between endoplasmic reticulum andGolgi compartments; inhibitor of genesilencing; cleaves ubiquitin fusions but notpolyubiquitin 0.001570.0251 S000005036YNL092WUncharacterizedPutative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene 0.001550.02429&radic S000005133SRP1YNL189WverifiedKaryopherin alpha homolog, forms a dimer with karyopherin betaKap95p to mediate import of nuclear proteins,binds the nuclear localization signal of thesubstrate during import; may also play a rolein regulation of protein degradation 0.001540.02392&radic&radic&radic S000003968RPL8BYLL045CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Ap and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 0.001530.02355&radic S000000993RPL14BYHL001WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl14Ap and has similarity to ratL14 ribosomal protein 0.001520.0232 S000004873TIF11YMR260CverifiedTranslation initiation factor eIF1A, essential protein thatforms a complex with Sui1p (eIF1) and the 40Sribosomal subunit and scans for the startcodon; C-terminus associates with Fun12p(eIF5B); N terminus interacts with eIF2 andeIF3 0.001520.02293&radic&radic S000000952SPI1YER150WverifiedGPI-anchored, serine/threonine rich cell wall protein ofunknown function; basal expression requiresMsn2p/Msn4p; expression is induced underconditions of stress and during the diauxicshift; similar to Sed1p 0.001510.02293 S000000955PET122YER153CverifiedSpecific translational activator for the COX3 mRNA that actstogether with Pet54p and Pet494p; located inthe mitochondrial inner membrane 0.00150.02226 S000004258SEC22YLR268WverifiedR-SNARE protein; assembles into SNARE complex with Bet1p, Bos1pand Sed5p; cycles between the ER and Golgicomplex; involved in anterograde and retrogradetransport between the ER and Golgi;synaptobrevin homolog 0.001490.02226&radic S000000197ECM21YBL101CverifiedNon-essential protein of unknown function; promoter containsseveral Gcn4p binding elements 0.00150.02226 S000004125SLX4YLR135WverifiedSubunit of a complex, with Slx1p, that hydrolyzes 5' branchesfrom duplex DNA in response to stalled orconverging replication forks; function overlapswith that of Sgs1p-Top3p 0.001490.02208&radic&radic S000001101FYV4YHR059WverifiedProtein of unknown function, required for survival uponexposure to K1 killer toxin 0.001480.02208 S000003454PET54YGR222WverifiedProtein required for splicing of the COX1 intron AI5 beta; alsospecifically required, together with Pet122pand Pet494p, for translation of the COX3 mRNA;located in the mitochondrial inner membrane 0.001490.02208 S000003419HGH1YGR187CverifiedProtein of unknown function with similarity to human HMG1 andHMG2; localizes to the cytoplasm 0.001470.02125 S000005487RPL25YOL127WverifiedPrimary rRNA-binding ribosomal protein component of the large(60S) ribosomal subunit, has similarity to E.coli L23 and rat L23a ribosomal proteins; bindsto 26S rRNA via a conserved C-terminal motif 0.001460.02097 S000003428FYV8YGR196CverifiedProtein of unknown function, required for survival uponexposure to K1 killer toxin 0.001460.02097&radic&radic S000005152YNL208WUncharacterizedHypothetical protein 0.001440.02057 S000001169YHR127WVerifiedProtein of unknown function; localizes to the nucleus 0.001450.02057&radic S000004883RRN9YMR270CverifiedProtein involved in promoting high level transcription of rDNA,subunit of UAF (upstream activation factor) forRNA polymerase I 0.001440.02031&radic&radic&radic S000006278TKL1YPR074CverifiedTransketolase, similar to Tkl2p; catalyzes conversion ofxylulose-5-phosphate and ribose-5-phosphate tosedoheptulose-7-phosphate andglyceraldehyde-3-phosphate in the pentosephosphate pathway; needed for synthesis ofaromatic amino acids 0.001440.02013&radic S000000044YAL046CUncharacterizedHypothetical protein 0.001430.02013&radic S000005101IGO1YNL157WuncharacterizedHypothetical protein 0.001420.01942&radic&radic S000002358YDL199CUncharacterizedPutative protein of unknown function with sequence similarity to members of the sugar permease family 0.001410.01936 S000002620TCP1YDR212WverifiedAlpha subunit of chaperonin-containing T-complex, whichmediates protein folding in the cytosol;involved in maintenance of actin cytoskeleton;homolog to Drosophila melanogaster and mousetailless complex polypeptide 0.001410.01936&radic&radic S000000472MRPL37YBR268WverifiedMitochondrial ribosomal protein of the large subunit 0.001410.01883&radic S000001643ELF1YKL160WverifiedTranscription elongation factor that contains a conserved zincfinger domain; implicated in the maintenance ofproper chromatin structure in activelytranscribed regions; deletion inhibits Bromemosaic virus (BMV) gene expression 0.00140.01883&radic&radic&radic S000004294MET17YLR303WverifiedO-acetyl homoserine-O-acetyl serine sulfhydrylase, required forsulfur amino acid synthesis 0.001390.01872 S000004297UBC12YLR306WverifiedEnzyme that mediates the conjugation of Rub1p, a ubiquitin-likeprotein, to other proteins; related to E2ubiquitin-conjugating enzymes 0.001390.01872 S000002240RPL13AYDL082WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl13Bp; not essential forviability; has similarity to rat L13 ribosomalprotein 0.001390.0185 S000005257YNL313CUncharacterizedProtein required for cell viability 0.001390.0185&radic S000004890FCP1YMR277WverifiedCarboxy-terminal domain (CTD) phosphatase, essential fordephosphorylation of the repeated C-terminaldomain of the RNA polymerase II large subunit(Rpo21p) 0.001370.01803&radic&radic&radic S000001283RPB3YIL021WverifiedRNA polymerase II third largest subunit B44, part of centralcore; similar to prokaryotic alpha subunit 0.001360.01781&radic S000003411OKP1YGR179CverifiedOuter kinetochore protein, required for accurate mitoticchromosome segregation; component of thekinetochore sub-complex COMA (Ctf19p, Okp1p,Mcm21p, Ame1p) that functions as a platform forkinetochore assembly 0.001360.01781&radic&radic S000003090NAB2YGL122CverifiedNuclear polyadenylated RNA-binding protein; autoregulates mRNAlevels; related to human hnRNPs; has nuclearlocalization signal sequence that binds toKap104p; required for poly(A) tail lengthcontrol and nuclear mRNA export 0.001360.01756&radic&radic S000001055ARD1YHR013CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.001350.01724 S000004490TSA1YML028WverifiedUbiquitous housekeeping thioredoxin peroxidase, reducesreactive oxygen, nitrogen and sulfur speciesusing thioredoxin as hydrogen donor; mediatesredox regulation of the nuclear localization ofYap1p; deletion results in mutator phenotype 0.001340.01685 S000001489RPL14AYKL006WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl14Bpand has similarity to rat L14 ribosomalprotein; rpl14a csh5 double null mutantexhibits synthetic slow growth 0.001340.01685 S000000893MET6YER091CverifiedCobalamin-independent methionine synthase, involved in aminoacid biosynthesis; requires a minimum of twoglutamates on the methyltetrahydrofolatesubstrate, similar to bacterial metE homologs 0.001310.01621 S000000029GIP4YAL031CverifiedCytoplasmic Glc7-interacting protein whose overexpressionrelocalizes Glc7p from the nucleus and preventschromosome segregation; potential Cdc28psubstrate 0.001310.01621&radic&radic S000005182KEX2YNL238WverifiedSubtilisin-like protease (proprotein convertase), acalcium-dependent serine protease involved inthe activation of proproteins of the secretorypathway 0.001310.01601&radic&radic S000006212HAA1YPR008WverifiedTranscriptional activator involved in the transcription ofTPO2, HSP30 and other genes encoding membranestress proteins; despite sequence similaritywith the transcription factor Ace1p, it is notsubject to metalloregulation 0.001280.01518 S000004421CRN1YLR429WverifiedCoronin, cortical actin cytoskeletal component that associateswith the Arp2p/Arp3p complex to regulate itsactivity 0.001260.01463 S000003348SPT6YGR116WverifiedEssential protein that interacts with histones and is involvedin nucleosome disassembly and reassembly duringtranscription elongation 0.001260.01456&radic S000001551NUP100YKL068WverifiedSubunit of the nuclear pore complex (NPC) that is localized toboth sides of the pore; contains a repetitiveGLFG motif that interacts with mRNA exportfactor Mex67p and with karyopherin Kap95p;homologous to Nup116p 0.001250.0144&radic&radic S000006002RPS9AYPL081WverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps9Bp and has similarity to E.coli S4 and rat S9 ribosomal proteins 0.001240.01408&radic S000004636ARP9YMR033WverifiedActin-related protein involved in transcriptional regulation;subunit of the chromatin remodeling Snf/Swicomplex 0.001240.01401 S000000106YBL010CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles 0.001240.01384&radic&radic S000004989YIP3YNL044WverifiedProtein localized to COPII vesicles, proposed to be involved inER to Golgi transport; interacts with membersof the Rab GTPase family and Yip1p; alsointeracts with Rtn1p 0.001230.01374 S000003202SEC15YGL233WverifiedEssential 113kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which mediates polarized targeting ofvesicles to active sites of exocytosis; Sec15passociates with Sec4p and vesicles 0.001230.01368&radic&radic S000004416SPP382YLR424WverifiedEssential protein that forms a dimer with Ntr2p; also forms atrimer, with Ntr2p and Prp43p, that is involvedin spliceosome disassembly; found also in amultisubunit complex with the splicing factorClf1p; suppressor of prp38-1 mutation 0.001210.01322&radic S000002982PUF4YGL014WverifiedMember of the PUF protein family, which is defined by thepresence of Pumilio homology domains thatconfer RNA binding activity; preferentiallybinds mRNAs encoding nucleolar ribosomalRNA-processing factors 0.001190.0125&radic&radic S000006284TEF1YPR080WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF2; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.001190.01236 S000001071YHI9YHR029CverifiedProtein of unknown function that is a member of the PhzFsuperfamily, although unlike its bacterialhomolog, is most likely not involved inphenazine production; possibly involved in amembrane regulation metabolic pathway 0.001180.01221&radic&radic S000004072SRL2YLR082CverifiedProtein of unknown function; overexpression suppresses thelethality caused by a rad53 null mutation 0.001180.01221&radic&radic&radic S000000951PEA2YER149CverifiedCoiled-coil polarisome protein required for polarizedmorphogenesis, cell fusion, and low affinityCa2+ influx; forms polarisome complex withBni1p, Bud6p, and Spa2p; localizes to sites ofpolarized growth 0.001180.01214 S000000019CCR4YAL021CverifiedComponent of the CCR4-NOT transcriptional complex, which isinvolved in regulation of gene expression;component of the major cytoplasmic deadenylase,which is involved in mRNA poly(A) tailshortening 0.001170.01188&radic&radic S000000370TYR1YBR166CverifiedPrephenate dehydrogenase involved in tyrosine biosynthesis,expression is dependent on phenylalanine levels 0.001170.01188 S000001404CCT2YIL142WverifiedSubunit beta of the cytosolic chaperonin Cct ring complex,related to Tcp1p, required for the assembly ofactin and tubulins in vivo 0.001170.0118 S000005180SIN4YNL236WverifiedSubunit of the Mediator complex; interacts with the RNApolymerase II holoenzyme to postively ornegatively regulate transcription; dispensiblefor basal transcription 0.001160.01161&radic&radic&radic S000001025RPL8AYHL033CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Bp and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 0.001150.01143 S000000307SIF2YBR103WverifiedWD40 repeat-containing subunit of the Set3C histone deacetylasecomplex, which represses early/middlesporulation genes; antagonizes telomericsilencing; binds specifically to the Sir4pN-terminus 0.001140.01106&radic S000005619YOR093CUncharacterizedHypothetical protein 0.001130.01089&radic&radic&radic S000004520IMD4YML056CverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.001130.01089 S000002856ADA2YDR448WverifiedTranscription coactivator, component of the ADA and SAGAtranscriptional adaptor/HAT (histoneacetyltransferase) complexes 0.00110.00996&radic&radic S000000322TEF2YBR118WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF1; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.00110.00983 S000003236PEX31YGR004WverifiedPeroxisomal integral membrane protein, involved in negativeregulation of peroxisome size; partiallyfunctionally redundant with Pex30p and Pex32p;probably acts at a step downstream of stepsmediated by Pex28p and Pex29p 0.00110.00983&radic&radic S000005013RPL16BYNL069CverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, binds to 5.8 S rRNA; hassimilarity to Rpl16Ap, E. coli L13 and rat L13aribosomal proteins; transcriptionally regulatedby Rap1p 0.001070.00895 S000004415ATG17YLR423CverifiedProtein that interacts with and is required for activation ofApg1p protein kinase; involved in autophagy butnot in the Cvt (cytoplasm to vacuole targeting)pathway 0.001060.00883 S000000358RPB5YBR154CverifiedRNA polymerase subunit ABC27, common to RNA polymerases I, II,and III; contacts DNA and affectstransactivation 0.001060.00876 S000003367PRE9YGR135Wverified20S proteasome beta-type subunit; the only nonessential 20Ssubunit 0.001060.00871&radic&radic&radic S000002207KNH1YDL049CverifiedProtein with similarity to Kre9p, which is involved in cellwall beta 1,6-glucan synthesis; overproductionsuppresses growth defects of a kre9 null mutant 0.001050.00857 S000006253ATG11YPR049CverifiedPeripheral membrane protein required for delivery ofaminopeptidase I (Lap4p) to the vacuole in thecytoplasm-to-vacuole targeting pathway; alsorequired for peroxisomal degradation(pexophagy) 0.001050.00835 S000004402VIP1YLR410WverifiedProtein of unknown function probably involved in the functionof the cortical actin cytoskeleton; putativeortholog of S. pombe asp1+ 0.001040.00832&radic S000004429YLR437CUncharacterizedPutative protein of unknown function; epitope tagged protein localizes to the cytoplasm 0.001040.00831 S000002946PAD1YDR538WverifiedPhenylacrylic acid decarboxylase, confers resistance tocinnamic acid, decarboxylates aromaticcarboxylic acids to the corresponding vinylderivatives 0.001030.00804 S000005589RPL3YOR063WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to E. coli L3 and rat L3 ribosomalproteins; involved in the replication andmaintenance of killer double stranded RNA virus 0.001030.008&radic&radic S000000564KRR1YCL059CverifiedEssential nucleolar protein required for the synthesis of 18SrRNA and for the assembly of 40S ribosomalsubunit 0.001030.0079&radic&radic S000000945DDI1YER143WverifiedDNA damage-inducible v-SNARE binding protein, contains aubiquitin-associated (UBA) domain, may act as anegative regulator of constitutive exocytosis,may play a role in S-phase checkpoint control 0.001030.0079 S000002377YDL218WUncharacterizedHypothetical protein 0.001030.0079 S000002600NUP42YDR192CverifiedSubunit of the nuclear pore complex (NPC) that localizesexclusively to the cytoplasmic side; involvedin RNA export, most likely at a terminal step;interacts with Gle1p 0.001020.00776 S000001177YCK1YHR135CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck2p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck2p 0.001020.00772&radic&radic S000004735ECM16YMR128WverifiedEssential DEAH-box ATP-dependent RNA helicase specific to theU3 snoRNP, predominantly nucleolar indistribution, required for 18S rRNA synthesis 0.001010.00763&radic&radic S000004041FCF2YLR051CverifiedEssential nucleolar protein involved in the early steps of 35SrRNA processing; interacts with Faf1p; memberof a transcriptionally co-regulated set ofgenes called the RRB regulon 0.001010.00763&radic&radic S000000542SRO9YCL037CverifiedCytoplasmic RNA-binding protein that associates withtranslating ribosomes; involved in hemeregulation of Hap1p as a component of the HMCcomplex, also involved in the organization ofactin filaments; contains a La motif 0.001010.00757&radic&radic S000001352ICE2YIL090WverifiedIntegral ER membrane protein with type-III transmembranedomains; mutations cause defects in cortical ERmorphology in both the mother and daughtercells 0.000990.00732&radic S000000439YBR235WUncharacterizedPutative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene 0.000990.00722&radic&radic S000006081CDC60YPL160WverifiedCytosolic leucyl tRNA synthetase, ligates leucine to theappropriate tRNA 0.000980.0072&radic S000000409KTR3YBR205WverifiedPutative alpha-1,2-mannosyltransferase involved in O- andN-linked protein glycosylation; member of theKRE2/MNT1 mannosyltransferase family 0.000980.00717&radic&radic S000000884UTP7YER082CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000980.0071&radic&radic&radic S000002707BFR2YDR299WverifiedEssential protein possibly involved in secretion; multicopysuppressor of sensitivity to Brefeldin A 0.000980.0071&radic&radic&radic S000005231SEC21YNL287WverifiedGamma subunit of coatomer, a heptameric protein complex thattogether with Arf1p forms the COPI coat;involved in ER to Golgi transport of selectivecargo 0.000970.00707&radic S000001239UTP9YHR196WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000970.00705&radic&radic S000001191IMP3YHR148WverifiedComponent of the SSU processome, which is required for pre-18SrRNA processing, essential protein thatinteracts with Mpp10p and mediates interactionsof Imp4p and Mpp10p with U3 snoRNA 0.000970.00701&radic&radic&radic S000005937SWI1YPL016WverifiedSubunit of the SWI/SNF chromatin remodeling complex, whichregulates transcription by remodelingchromosomes; required for transcription of manygenes, including ADH1, ADH2, GAL1, HO, INO1 andSUC2 0.000960.00692&radic&radic&radic S000005398PRE6YOL038Wverified20S proteasome alpha-type subunit; green fluorescent protein(GFP)-fusion protein relocates from the cytosolto the mitochondrial outer surface uponoxidative stress 0.000950.00672&radic&radic&radic S000005326MVD1YNR043WverifiedMevalonate pyrophosphate decarboxylase, essential enzymeinvolved in the biosynthesis of isoprenoids andsterols, including ergosterol; acts as ahomodimer 0.000950.00672&radic S000000100UTP20YBL004WverifiedComponent of the small-subunit (SSU) processome, which isinvolved in the biogenesis of the 18S rRNA 0.000950.00669&radic&radic S000006047NAN1YPL126WverifiedU3 snoRNP protein, component of the small (ribosomal) subunit(SSU) processosome containing U3 snoRNA;required for the biogenesis of18S rRNA 0.000940.00663&radic S000001582UTP11YKL099CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000940.00663&radic&radic S000004643YET2YMR040WverifiedProtein of unknown function that may interact with ribosomes,based on co-purification experiments; homologof human BAP31 protein 0.000940.0066&radic&radic S000002773ESF1YDR365CverifiedNucleolar protein involved in pre-rRNA processing; depletioncauses severely decreased 18S rRNA levels 0.000940.00656&radic&radic&radic&radic&radic S000002767VID21YDR359CverifiedComponent of the NuA4 histone acetyltransferase complex 0.000940.00654&radic&radic S000003377ENP2YGR145WuncharacterizedEssential nucleolar protein of unknown function; contains WDrepeats, interacts with Mpp10p and Bfr2p, andhas homology to Spb1p 0.000930.00644&radic&radic S000002732UTP4YDR324CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.000930.00644&radic&radic&radic&radic S000004762NUP53YMR153WverifiedSubunit of the nuclear pore complex (NPC), interacts withkaryopherin Kap121p or with Nup170p viaoverlapping regions of Nup53p, involved inactivation of the spindle checkpoint mediatedby the Mad1p-Mad2p complex 0.000920.00634&radic&radic S000006082BEM4YPL161CverifiedProtein involved in establishment of cell polarity and budemergence; interacts with the Rho1p smallGTP-binding protein and with the Rho-typeGTPase Cdc42p; involved in maintenance ofproper telomere length 0.000920.00631&radic&radic&radic S000003088PRP43YGL120CverifiedRNA helicase in the DEAH-box family, functions in both RNApolymerase I and polymerase II transcriptmetabolism, involved in release of thelariat-intron from the spliceosome 0.000910.0062&radic S000001768UTP30YKR060WuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.000910.00612&radic&radic S000001876LPD1YFL018CverifiedDihydrolipoamide dehydrogenase, the lipoamide dehydrogenasecomponent (E3) of the pyruvate dehydrogenaseand 2-oxoglutarate dehydrogenase multi-enzymecomplexes 0.000910.00612&radic&radic S000006124TPK2YPL203WverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; activates filamentous growth 0.00090.00608&radic&radic&radic S000002491TVP23YDR084CuncharacterizedIntegral membrane protein localized to late Golgi vesiclesalong with the v-SNARE Tlg2p; green fluorescentprotein (GFP)-fusion protein localizes to thecytoplasm in a punctate pattern 0.00090.00603&radic S000003220HFM1YGL251CverifiedMeiosis specific DNA helicase involved in the conversion ofdouble-stranded breaks to later recombinationintermediates and in crossover control;catalyzes the unwinding of Holliday junctions;has ssDNA and dsDNA stimulated ATPase activity 0.000890.00598&radic&radic S000005750RPB8YOR224CverifiedRNA polymerase subunit ABC14.5, common to RNA polymerases I,II, and III 0.000880.00585&radic&radic S000002505GRX3YDR098CverifiedHydroperoxide and superoxide-radical responsiveglutathione-dependent oxidoreductase; monothiolglutaredoxin subfamily member along with Grx4pand Grx5p; protects cells from oxidative damage 0.000880.0058&radic&radic&radic S000003700TPK1YJL164CverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; inhibits filamentous growth 0.000880.0058&radic&radic&radic S000005785RPT4YOR259CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for spindlepole body duplication; localized mainly to thenucleus throughout the cell cycle 0.000860.00567&radic&radic&radic&radic S000004674MOT3YMR070WverifiedNuclear transcription factor with two Cys2-His2 zinc fingers;involved in repression of a subset of hypoxicgenes by Rox1p, repression of several DAN/TIRgenes during aerobic growth, and repression ofergosterol biosynthetic genes 0.000840.00547&radic&radic S000004443LEU3YLR451WverifiedZinc-finger transcription factor that regulates genes involvedin branched chain amino acid biosynthesis andammonia assimilation; positively regulated byalpha-isopropylmalate, an intermediate inleucine biosynthesis 0.000840.00546&radic&radic S000000238HMT1YBR034CverifiedNuclear SAM-dependent mono- and asymmetric argininedimethylating methyltransferase that modifieshnRNPs, including Npl3p and Hrp1p, thusfacilitating nuclear export of these proteins;required for viability of npl3 mutants 0.000830.00542&radic&radic S000001942CNN1YFR046CverifiedKinetochore protein of unknown function; associated with theessential kinetochore proteins Nnf1p andSpc24p; phosphorylated by both Clb5-Cdk1 and,to a lesser extent, Clb2-Cdk1. 0.000830.00536&radic&radic&radic&radic S000003219YGL250WUncharacterizedPutative protein of unknown function; deletion mutant results in reduced PIS1 expression and has growth defects on non-fermentable carbon sources and on minimal media; GFP-fusion protein localizes to both the cytoplasm and the nucleus 0.000830.00535&radic&radic&radic S000005361PHO80YOL001WverifiedCyclin, negatively regulates phosphate metabolism;Pho80p-Pho85p (cyclin-CDK complex)phosphorylates Pho4p and Swi5p; deletion ofPHO80 leads to aminoglycoside supersensitivity;truncated form of PHO80 affects vacuoleinheritance 0.000820.00531&radic&radic S000004798HSC82YMR186WverifiedCytoplasmic chaperone of the Hsp90 family, redundant infunction and nearly identical with Hsp82p, andtogether they are essential; expressedconstitutively at 10-fold higher basal levelsthat HSP82 and induced 2-3 fold by heat shock 0.000820.00531&radic&radic S000003500HUA1YGR268CuncharacterizedCytoplasmic protein containing a zinc finger domain withsequence similarity to that of Type IJ-proteins; computational analysis oflarge-scale protein-protein interaction datasuggests a possible role in actin patchassembly 0.000820.00528&radic&radic&radic S000006372NUT2YPR168WverifiedComponent of the RNA polymerase II mediator complex, which isrequired for transcriptional activation andalso has a role in basal transcription 0.000810.00524&radic&radic&radic&radic S000004808ICY1YMR195WuncharacterizedProtein of unknown function, required for viability in richmedia of cells lacking mitochondrial DNA 0.000810.00524&radic&radic S000004912PRC1YMR297WverifiedVacuolar carboxypeptidase Y (proteinase C), involved in proteindegradation in the vacuole and required forfull protein degradation during sporulation 0.000810.0052&radic&radic S000000339CKS1YBR135WverifiedSubunit of the Cdc28 protein kinase, required for mitoticproteolysis, may also be involved in theproteolysis of the G1 cyclins 0.00080.00517&radic&radic S000000402TAF5YBR198CverifiedSubunit (90 kDa) of TFIID and SAGA complexes, involved in RNApolymerase II transcription initiation and inchromatin modification 0.00080.00511&radic&radic&radic&radic S000006175HFI1YPL254WverifiedAdaptor protein required for structural integrity of the SAGAcomplex, a histoneacetyltransferase-coactivator complex that isinvolved in global regulation of geneexpression through acetylation andtranscription functions 0.00080.00511&radic&radic S000002665SET7YDR257CverifiedNuclear protein that contains a SET-domain, which have beenshown to mediate methyltransferase activity inother proteins 0.000790.00509&radic&radic&radic&radic S000006125HRR25YPL204WverifiedProtein kinase involved in regulating diverse events includingvesicular trafficking, gene expression, DNArepair, and chromosome segregation; binds theCTD of RNA pol II; homolog of mammalian caseinkinase 1delta (CK1delta) 0.000790.00507&radic&radic&radic&radic S000005388YAP7YOL028CverifiedPutative basic leucine zipper (bZIP) transcription factor 0.000790.00505&radic&radic&radic&radic S000005577YOR051CUncharacterizedNuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts 0.000790.00503&radic&radic S000005739SAS5YOR213CverifiedSubunit of the SAS complex (Sas2p, Sas4p, Sas5p), whichacetylates free histones and nucleosomes andregulates transcriptional silencing; stimulatesSas2p HAT activity 0.000790.00503&radic&radic&radic&radic S000003152STR3YGL184CverifiedCystathionine beta-lyase, converts cystathionine intohomocysteine 0.000780.005&radic S000004391BDF1YLR399CverifiedProtein involved in transcription initiation at TATA-containingpromoters; associates with the basaltranscription factor TFIID; contains twobromodomains; corresponds to the C-terminalregion of mammalian TAF1; redundant with Bdf2p 0.000780.005&radic&radic S000004249HSP60YLR259CverifiedTetradecameric mitochondrial chaperonin required forATP-dependent folding of precursor polypeptidesand complex assembly; prevents aggregation andmediates protein refolding after heat shock;role in mtDNA transmission; similarity to groEL 0.000780.00495&radic S000005234RFC3YNL290WverifiedSubunit of heteropentameric Replication factor C (RF-C), whichis a DNA binding protein and ATPase that actsas a clamp loader of the proliferating cellnuclear antigen (PCNA) processivity factor forDNA polymerases delta and epsilon 0.000780.00495&radic&radic S000003874RSM7YJR113CverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S7 ribosomal protein 0.000780.00495 S000005985CWC27YPL064CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to S. pombeCwf27p 0.000770.00494&radic&radic&radic&radic S000005160RAP1YNL216WverifiedDNA-binding protein involved in either activation or repressionof transcription, depending on binding sitecontext; also binds telomere sequences andplays a role in telomeric position effect(silencing) and telomere structure 0.000770.00489&radic&radic&radic&radic S000005041PHO23YNL097CverifiedProbable component of the Rpd3 histone deacetylase complex,involved in transcriptional regulation of PHO5;C-terminus has similarity to human candidatetumor suppressor p33(ING1) 0.000770.00489&radic&radic S000000851TPA1YER049WuncharacterizedProtein of unknown function; interacts with Sup45p (eRF1),Sup35p (eRF3) and Pab1p; has a role intranslation termination efficiency, mRNApoly(A) tail length and mRNA stability 0.000760.00484&radic&radic S000002798UBA2YDR390CverifiedNuclear protein that acts as a heterodimer with Aos1p toactivate Smt3p (SUMO) before its conjugation toproteins (sumoylation), which may play a rolein protein targeting; essential for viability 0.000760.00484&radic&radic&radic&radic S000002508ARX1YDR101CverifiedShuttling pre-60S factor; involved in the biogenesis ofribosomal large subunit biogenesis; interactsdirectly with Alb1; responsible for Tif6recycling defects in absence of Rei1;associated with the ribosomal export complex 0.000760.00483&radic&radic S000002803SXM1YDR395WverifiedNuclear transport factor (karyopherin) involved in proteintransport between the cytoplasm andnucleoplasm; similar to Nmd5p, Cse1p, Lph2p,and the human cellular apoptosis susceptibilityprotein, CAS1 0.000750.00477&radic&radic&radic S000002284CDC48YDL126CverifiedATPase in ER, nuclear membrane and cytosol with homology tomammalian p97; in a complex with Npl4p andUfd1p participates in retrotranslocation ofubiquitinated proteins from the ER into thecytosol for degradation by the proteasome 0.000750.00477&radic&radic&radic S000005190VPS75YNL246WverifiedProtein of unknown function involved in vacuolar proteinsorting; detected in the nucleus 0.000750.00477&radic&radic&radic S000006305SNT309YPR101WverifiedComponent of NineTeen complex (NTC) containing Prp19p involvedin mRNA splicing, interacts physically andgenetically with Prp19p 0.000750.00477&radic&radic S000002716SRB7YDR308CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation; target of theglobal repressor Tup1p 0.000740.00476&radic&radic&radic&radic S000001178SPL2YHR136CverifiedProtein with similarity to cyclin-dependent kinase inhibitors,overproduction suppresses a plc1 null mutation;green fluorescent protein (GFP)-fusion proteinlocalizes to the cytoplasm in a punctatepattern 0.000740.00473&radic S000004166RFX1YLR176CverifiedProtein involved in DNA damage and replication checkpointpathway; recruits repressors Tup1p and Cyc8p topromoters of DNA damage-inducible genes;similar to a family of mammalian DNA bindingRFX1-4 proteins 0.000740.00473&radic&radic&radic&radic S000005198YNL254CUncharacterizedHypothetical protein 0.000740.00473&radic&radic&radic S000003039AFT1YGL071WverifiedTranscription factor involved in iron utilization andhomeostasis; binds the consensus sitePyPuCACCCPu and activates the expression oftarget genes in response to changes in ironavailability 0.000740.00471&radic&radic&radic&radic S000001957SAE3YHR079C-AverifiedMeiosis specific protein involved in DMC1-dependent meioticrecombination, forms heterodimer with Mei5p;proposed to be an assembly factor for Dmc1p 0.000730.0047 S000004718MED11YMR112CverifiedEssential subunit of the RNA polymerase II mediator complex;associates with core polymerase subunits toform the RNA polymerase II holoenzyme 0.000730.0047&radic&radic S000000115APN2YBL019WverifiedClass II abasic (AP) endonuclease involved in repair of DNAdamage; homolog of human HAP1 and E. coliexoIII 0.000730.0047&radic&radic&radic S000003435YGR203WUncharacterizedPutative protein with sequence similarity to protein tyrosine phosphatases of the CDC25-like phosphatase family; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 0.000730.0047&radic&radic&radic S000003962UBI4YLL039CverifiedUbiquitin, becomes conjugated to proteins, marking them forselective degradation via the ubiquitin-26Sproteasome system; essential for the cellularstress response 0.000730.0047&radic&radic S000000562PRD1YCL057WverifiedZinc metalloendopeptidase, found in the cytoplasm andintermembrane space of mitochondria; withCym1p, involved in degradation of mitochondrialproteins and of presequence peptides cleavedfrom imported proteins 0.000730.00469&radic S000003601DLS1YJL065CverifiedSubunit of ISW2/yCHRAC chromatin accessibility complex alongwith Itc1p, Isw2p, and Dpb4p; involved ininheritance of telomeric silencing 0.000730.00467&radic&radic S000001505CDC16YKL022CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; required forsporulation 0.000730.00467&radic&radic&radic S000006010RLM1YPL089CverifiedMADS-box transcription factor, component of the protein kinaseC-mediated MAP kinase pathway involved in themaintenance of cell integrity; phosphorylatedand activated by the MAP-kinase Slt2p 0.000730.00467&radic&radic S000000680TUP1YCR084CverifiedGeneral repressor of transcription, forms complex with Cyc8p,involved in the establishment of repressivechromatin structure through interactions withhistones H3 and H4, appears to enhanceexpression of some genes 0.000730.00466&radic&radic S000004560RAD10YML095CverifiedSingle-stranded DNA endonuclease (with Rad1p), cleavessingle-stranded DNA during nucleotide excisionrepair and double-strand break repair; subunitof Nucleotide Excision Repair Factor 1 (NEF1);homolog of human ERCC1 protein 0.000720.00464&radic&radic S000005464NDJ1YOL104CverifiedMeiosis-specific telomere protein, required for bouquetformation, effective homolog pairing, orderedcross-over distribution (interference), sisterchromatid cohesion at meiotic telomeres, andsegregation of small chromosomes 0.000720.00464&radic S000001139YHR097CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus 0.000730.00464&radic&radic&radic&radic S000005959MET31YPL038WverifiedZinc-finger DNA-binding protein, involved in regulatingexpression of the methionine biosyntheticgenes, similar to Met32p 0.000720.00464&radic&radic&radic&radic S000003502YTA7YGR270WverifiedProtein of unknown function, member of CDC48/PAS1/SEC18 familyof ATPases, potentially phosphorylated byCdc28p 0.000730.00464&radic S000003813RAD7YJR052WverifiedProtein that recognizes and binds damaged DNA in anATP-dependent manner (with Rad16p) duringnucleotide excision repair; subunit ofNucleotide Excision Repair Factor 4 (NEF4) 0.000720.00463&radic&radic S000005159IES2YNL215WverifiedProtein that associates with the INO80 chromatin remodelingcomplex under low-salt conditions 0.000720.00463&radic&radic&radic&radic S000001197RTT107YHR154WverifiedProtein implicated in Mms22-dependent DNA repair during Sphase, DNA damage induces phosphorylation byMec1p at one or more SQ/TQ motifs; interactswith Mms22p and Slx4p; has four BRCT domains;has a role in regulation of Ty1 transposition 0.000720.00463&radic&radic S000002769BCP1YDR361CverifiedEssential protein involved in nuclear export of Mss4p, which isa lipid kinase that generatesphosphatidylinositol 4,5-biphosphate and playsa role in actin cytoskeleton organization andvesicular transport 0.000720.00463&radic&radic&radic S000006149CET1YPL228WverifiedBeta (RNA 5'-triphosphatase) subunit of the mRNA cappingenzyme, a heterodimer (the other subunit isCEG1, a guanylyltransferase) involved in addingthe 5' cap to mRNA; the mammalian enzyme is asingle bifunctional polypeptide 0.000720.00463&radic&radic S000002494RRP1YDR087CverifiedEssential evolutionarily conserved nucleolar protein necessaryfor biogenesis of 60S ribosomal subunits andprocessing of pre-rRNAs to mature rRNAs,associated with several distinct 66Spre-ribosomal particles 0.000720.00463&radic&radic&radic S000005756WTM1YOR230WverifiedTranscriptional repressor involved in regulation of meiosis andsilencing; contains WD repeats 0.000720.00463&radic&radic&radic S000004752YMR144WUncharacterizedPutative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene 0.000720.00462&radic&radic S000000974BRR2YER172CverifiedRNA-dependent ATPase RNA helicase involved in the facilitationand disruption of snRNA interactions, requiredfor disruption of U4/U6 base-pairing in nativesnRNPs to activate the spliceosome forcatalysis 0.000720.00461&radic&radic S000005006GCD10YNL062CverifiedSubunit of tRNA (1-methyladenosine) methyltransferase withGcd14p, required for the modification of theadenine at position 58 in tRNAs, especiallytRNAi-Met; first identified as a negativeregulator of GCN4 expression 0.000720.00461&radic&radic S000005755WTM2YOR229WverifiedTranscriptional repressor involved in regulation of meiosis andsilencing; contains WD repeats 0.000710.00459&radic&radic&radic S000004042IES3YLR052WverifiedSubunit of the INO80 chromatin remodeling complex 0.000710.00459&radic S000003177MIG2YGL209WverifiedProtein containing zinc fingers, involved in repression, alongwith Mig1p, of SUC2 (invertase) expression byhigh levels of glucose; binds to Mig1p-bindingsites in SUC2 promoter 0.000710.00459&radic&radic&radic&radic S000005879YOR352WUncharacterizedHypothetical protein 0.000720.00459&radic&radic&radic S000005236PUS4YNL292WverifiedPseudouridine synthase, catalyzes only the formation ofpseudouridine-55 (Psi55), a highly conservedtRNA modification, in mitochondrial andcytoplasmic tRNAs; PUS4 overexpression leads totranslational derepression of GCN4 (Gcd-phenotype) 0.000710.00459&radic&radic&radic S000002771ESC2YDR363WverifiedProtein involved in mating-type locus silencing, interacts withSir2p; probably functions to recruit orstabilize Sir proteins 0.000710.00459&radic&radic&radic S000003698JJJ2YJL162CuncharacterizedProtein of unknown function, contains a J-domain, which is aregion with homology to the E. coli DnaJprotein 0.000710.00458&radic&radic&radic&radic S000003050YGL082WUncharacterizedHypothetical protein 0.000710.00458&radic&radic&radic S000000488YBR284WUncharacterizedPutative protein of unknown function; YBR284W is not an essential gene; null mutant exhibits decreased resistance to rapamycin and wortmannin 0.000710.00456 S000004447YLR455WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR455W is not an essential gene 0.000710.00456&radic&radic S000001873GAT1YFL021WverifiedTranscriptional activator of genes involved in nitrogencatabolite repression, member of the GATAfamily of DNA binding proteins; activity andlocalization regulated by nitrogen limitationand Ure2p 0.000710.00456&radic&radic&radic S000000362AMN1YBR158WverifiedProtein required for daughter cell separation, multiple mitoticcheckpoints, and chromosome stability; contains12 degenerate leucine-rich repeat motifs;expression is induced by the Mitotic ExitNetwork (MEN) 0.000710.00456&radic&radic S000001384POG1YIL122WverifiedPutative transcriptional activator that promotes recovery frompheromone induced arrest; inhibits bothalpha-factor induced G1 arrest and repressionof CLN1 and CLN2 via SCB/MCB promoter elements;potential Cdc28p substrate; SBF regulated 0.000710.00456&radic&radic S000004012SDO1YLR022CuncharacterizedEssential protein involved in RNA metabolism, one of two yeasthomologs (with Yhr087wp) of the human proteinSBDS responsible for autosomal recessiveShwachman-Bodian-Diamond Syndrome, alsoconserved in Archaea 0.000710.00456&radic&radic S000002450NRG1YDR043CverifiedTranscriptional repressor that recruits the Cyc8p-Tup1p complexto promoters; mediates glucose repression andnegatively regulates a variety of processesincluding filamentous growth and alkaline pHresponse 0.000710.00456&radic&radic S000005233PCL1YNL289WverifiedPho85 cyclin of the Pcl1,2-like subfamily, involved in entryinto the mitotic cell cycle and regulation ofmorphogenesis, localizes to sites of polarizedcell growth 0.000710.00456&radic&radic S000004250LCB5YLR260WverifiedMinor sphingoid long-chain base kinase, paralog of Lcb4presponsible for few percent of the totalactivity, possibly involved in synthesis oflong-chain base phosphates, which function assignaling molecules 0.000710.00455&radic S000001407PAN6YIL145CverifiedPantothenate synthase, also known as pantoate-beta-alanineligase, required for pantothenic acidbiosynthesis, deletion causes pantothenic acidauxotrophy, homologous to E. coli panC 0.000710.00454&radic S000001717FOX2YKR009CverifiedMultifunctional enzyme of the peroxisomal fatty acidbeta-oxidation pathway; has 3-hydroxyacyl-CoAdehydrogenase and enoyl-CoA hydrataseactivities 0.00070.00454 S000005428RTG1YOL067CverifiedTranscription factor (bHLH) involved in interorganellecommunication between mitochondria,peroxisomes, and nucleus 0.00070.00453&radic&radic&radic S000000678AHC2YCR082WuncharacterizedProtein of unknown function, putative transcriptionalregulator; proposed to be a Ada Histoneacetyltransferase complex component; GFP taggedprotein is localized to the cytoplasm andnucleus 0.00070.00453&radic&radic S000001293ULP2YIL031WverifiedPeptidase that deconjugates Smt3/SUMO-1 peptides from proteins,plays a role in chromosome cohesion atcentromeric regions and recovery fromcheckpoint arrest induced by DNA damage or DNAreplication defects; potential Cdc28p substrate 0.00070.00451 S000003925RTT109YLL002WverifiedProtein with a role in regulation of Ty1 transposition 0.00070.0045&radic&radic S000002290CDC53YDL132WverifiedCullin, structural protein of SCF complexes (which also containSkp1p, Cdc34p, and an F-box protein) involvedin ubiquitination; SCF promotes the G1-Stransition by targeting G1 cyclins and theCln-CDK inhibitor Sic1p for degradation 0.00070.0045&radic&radic S000001111RRP4YHR069CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp43p and Dis3p 0.00070.00449&radic&radic&radic S000000961BUR6YER159CverifiedProtein that forms a heterodimeric histone-fold NC2 generaltranscription regulator complex with Ydr1p thatbinds to TBP and represses RNA pol IItranscription during assembly of thepreinitiation complex, homologous to humanNC2alpha 0.00070.00449&radic&radic S000004365VID22YLR373CverifiedGlycosylated integral membrane protein localized to the plasmamembrane; plays a role infructose-1,6-bisphosphatase (FBPase)degradation; involved in FBPase transport fromthe cytosol to Vid (vacuole import anddegradation) vesicles 0.000690.00448&radic&radic S000001946PRE4YFR050Cverified20S proteasome beta-type subunit 0.000690.00448&radic&radic S000004276CTS1YLR286CverifiedEndochitinase, required for cell separation after mitosis;transcriptional activation during late G andearly M cell cycle phases is mediated bytranscription factor Ace2p 0.000690.00447 S000005887PDE2YOR360CverifiedHigh-affinity cyclic AMP phosphodiesterase, component of thecAMP-dependent protein kinase signaling system,protects the cell from extracellular cAMP,contains readthrough motif surroundingtermination codon 0.000690.00446&radic&radic&radic S000001597APN1YKL114CverifiedMajor apurinic/apyrimidinic endonuclease, 3'-repair diesteraseinvolved in repair of DNA damage by oxidationand alkylating agents; also functions as a3'-5' exonuclease to repair7,8-dihydro-8-oxodeoxyguanosine 0.000690.00446&radic&radic&radic&radic S000001749YKR041WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.000690.00446&radic&radic S000005253STB1YNL309WverifiedProtein with a role in regulation of MBF-specific transcriptionat Start, phosphorylated by Cln-Cdc28p kinasesin vitro; unphosphorylated form binds Swi6p andbinding is required for Stb1p function;expression is cell-cycle regulated 0.000690.00445&radic&radic S000001249SKN7YHR206WverifiedNuclear response regulator and transcription factor, part of abranched two-component signaling system;required for optimal induction of heat-shockgenes in response to oxidative stress; involvedin osmoregulation 0.000690.00445&radic&radic&radic S000005491YOL131WUncharacterizedPutative protein of unknown function 0.000690.00443 S000005248YPT11YNL304WverifiedRab-type small GTPase that interacts with the C-terminal taildomain of Myo2p to mediate distribution ofmitochondria to daughter cells 0.000690.00443&radic&radic S000004590GTR1YML121WverifiedCytoplasmic GTP binding protein and negative regulator, withhomolog Gtr2p, of the Ran/Tc4 GTPase cycle;component of GSE complex, which is required forsorting of Gap1p; involved in phosphatetransport; has homology to human RagA and RagB 0.000690.00443 S000003412RNR4YGR180CverifiedRibonucleotide-diphosphate reductase (RNR), small subunit; theRNR complex catalyzes the rate-limiting step indNTP synthesis and is regulated by DNAreplication and DNA damage checkpoint pathwaysvia localization of the small subunits 0.000690.00443&radic&radic&radic&radic S000004265SMD2YLR275WverifiedCore Sm protein Sm D2; part of heteroheptameric complex (withSmb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D2 0.000690.00443&radic&radic&radic S000000890DOT6YER088CverifiedProtein of unknown function, involved in telomeric genesilencing and filamentation 0.000690.00443&radic&radic S000005460PKH2YOL100WverifiedSerine/threonine protein kinase involved insphingolipid-mediated signaling pathway thatcontrols endocytosis; activates Ypk1p andYkr2p, components of signaling cascade requiredfor maintenance of cell wall integrity;redundant with Pkh1p 0.000690.00443 S000005484TRM11YOL124CverifiedCatalytic subunit of an adoMet-dependent tRNA methyltransferasecomplex (Trm11p-Trm112p), required for themethylation of the guanosine nucleotide atposition 10 (m2G10) in tRNAs; contains a THUMPdomain and a methyltransferase domain 0.000680.00442 S000003361SYF2YGR129WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression 0.000680.0044&radic&radic&radic S000005621RKI1YOR095CverifiedRibose-5-phosphate ketol-isomerase, catalyzes theinterconversion of ribose 5-phosphate andribulose 5-phosphate in the pentose phosphatepathway; participates in pyridoxinebiosynthesis 0.000680.0044&radic&radic S000000530AGP1YCL025CverifiedLow-affinity amino acid permease with broad substrate range,involved in uptake of asparagine, glutamine,and other amino acids; expression is regulatedby the SPS plasma membrane amino acid sensorsystem (Ssy1p-Ptr3p-Ssy5p) 0.000680.0044&radic&radic S000000657YCR061WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 0.000680.0044 S000004064BUD20YLR074CverifiedProtein involved in bud-site selection; diploid mutants displaya random budding pattern instead of thewild-type bipolar pattern 0.000680.0044&radic&radic&radic S000003262POP6YGR030CverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.000680.0044&radic&radic&radic S000001785YKR077WUncharacterizedPutative transcriptional activator, identified by high-throughput two-hybrid experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YKR077W is not an essential gene 0.000680.00439&radic&radic&radic S000001789RPF2YKR081CverifiedEssential protein involved in the processing of pre-rRNA andthe assembly of the 60S ribosomal subunit;interacts with ribosomal protein L11; localizespredominantly to the nucleolus; constituent of66S pre-ribosomal particles 0.000680.00438&radic&radic S000003041HSF1YGL073WverifiedTrimeric heat shock transcription factor, activates multiplegenes in response to stresses that includehyperthermia; recognizes variable heat shockelements (HSEs) consisting of inverted NGAANrepeats; posttranslationally regulated 0.000680.00438&radic&radic&radic&radic S000001106SSZ1YHR064CverifiedHsp70 protein that interacts with Zuo1p (a DnaJ homolog) toform a ribosome-associated complex that bindsthe ribosome via the Zuo1p subunit; alsoinvolved in pleiotropic drug resistance viasequential activation of PDR1 and PDR5; bindsATP 0.000680.00438 S000004876SAP30YMR263WverifiedSubunit of a histone deacetylase complex, along with Rpd3p andSin3p, that is involved in silencing attelomeres, rDNA, and silent mating-type loci;involved in telomere maintenance 0.000680.00438&radic&radic&radic S000004911LCB1YMR296CverifiedComponent of serine palmitoyltransferase, responsible alongwith Lcb2p for the first committed step insphingolipid synthesis, which is thecondensation of serine with palmitoyl-CoA toform 3-ketosphinganine 0.000670.00436&radic S000001954MEI4YER044C-AverifiedMeiosis-specific protein involved in recombination; requiredfor chromosome synapsis; required forproduction of viable spores 0.000670.00436&radic&radic S000005996GCR1YPL075WverifiedTranscriptional activator of genes involved in glycolysis;DNA-binding protein that interacts andfunctions with the transcriptional activatorGcr2p 0.000670.00436&radic&radic&radic S000004984BDP1YNL039WverifiedEssential subunit of RNA polymerase III transcription factor(TFIIIB), which is involved in transcription ofgenes encoding tRNAs, 5S rRNA, U6 snRNA, andother small RNAs 0.000670.00433&radic&radic S000002872SPP41YDR464WverifiedProtein involved in negative regulation of expression ofspliceosome components PRP4 and PRP3 0.000670.00433&radic&radic S000001668ASH1YKL185WverifiedZinc-finger inhibitor of HO transcription; mRNA is localizedand translated in the distal tip of anaphasecells, resulting in accumulation of Ash1p indaughter cell nuclei and inhibition of HOexpression; potential Cdc28p substrate 0.000670.00433&radic&radic S000006347RRP15YPR143WverifiedNucleolar protein, constituent of pre-60S ribosomal particles;required for processing of the 27S pre-rRNA atthe A2 site to yield 5.8S and 25S rRNA 0.000670.00433&radic&radic&radic S000001608RRN3YKL125WverifiedProtein required for transcription of rDNA by RNA polymerase I;transcription factor independent of DNAtemplate; involved in recruitment of RNApolymerase I to rDNA 0.000670.00433&radic&radic&radic S000003658ALB1YJL122WverifiedShuttling pre-60S factor; involved in the biogenesis ofribosomal large subunit; interacts directlywith Arx1p; responsible for Tif6p recyclingdefects in absence of Rei1p. 0.000670.00431&radic&radic S000006373JIP5YPR169WuncharacterizedNucleolar protein of unknown function, exhibits a physicalinteraction with Bre1p 0.000670.00431&radic&radic&radic&radic S000004927YMR310CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene 0.000670.00431&radic&radic&radic&radic S000005468INO4YOL108CverifiedTranscription factor required for derepression ofinositol-choline-regulated genes involved inphospholipid synthesis; forms a complex, withIno2p, that binds theinositol-choline-responsive element through abasic helix-loop-helix domain 0.000670.00431&radic&radic S000003322UTP22YGR090WuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.000660.00428&radic&radic&radic&radic&radic S000000111ACH1YBL015WverifiedAcetyl-coA hydrolase, primarily localized to mitochondria;required for acetate utilization and fordiploid pseudohyphal growth 0.000660.00428 S000000981DMC1YER179WverifiedMeiosis-specific protein required for repair of double-strandbreaks and pairing between homologouschromosomes; homolog of Rad51p and thebacterial RecA protein 0.000660.00428 S000005226POP3YNL282WverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.000660.00427&radic&radic&radic&radic S000005461IZH4YOL101CverifiedMembrane protein involved in zinc metabolism, member of thefour-protein IZH family, expression induced byfatty acids and altered zinc levels; deletionreduces sensitivity to excess zinc; possiblerole in sterol metabolism 0.000660.00427 S000002413SOK1YDR006CverifiedProtein whose overexpression suppresses the growth defect ofmutants lacking protein kinase A activity;involved in cAMP-mediated signaling; localizedto the nucleus; similar to the mousetestis-specific protein PBS13 0.000660.00426&radic&radic S000003410PBP1YGR178CverifiedProtein interacting with poly(A)-binding protein Pab1p; likelyinvolved in controlling the extent of mRNApolyadenylation; forms a complex with Mkt1pthat may regulate HO translation; interactswith Lsm12p in a copurification assay 0.000660.00426&radic&radic&radic&radic S000004098YLR108CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene 0.000660.00426&radic&radic&radic S000006312RPN7YPR108WverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome,similar to another S. cerevisiae regulatorysubunit, Rpn5p, as well as to mammalianproteasome subunits 0.000650.00425&radic&radic&radic&radic&radic S000003563RNR2YJL026WverifiedRibonucleotide-diphosphate reductase (RNR), small subunit; theRNR complex catalyzes the rate-limiting step indNTP synthesis and is regulated by DNAreplication and DNA damage checkpoint pathwaysvia localization of the small subunits 0.000650.00425&radic S000001082BCD1YHR040WverifiedEssential protein required for the accumulation of box C/DsnoRNA 0.000650.00425&radic&radic&radic&radic S000003437YGR205WVerifiedATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases 0.000650.00425&radic&radic S000004289YHC1YLR298CverifiedComponent of the U1 snRNP complex required for pre-mRNAsplicing; putative ortholog of human U1Cprotein, which is involved in formation of acomplex between U1 snRNP and the pre-mRNA 5'splice site 0.000650.00425&radic&radic S000000099HTA2YBL003CverifiedOne of two nearly identical (see also HTA1) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.000650.00425&radic&radic&radic&radic&radic S000000566MRC1YCL061CverifiedS-phase checkpoint protein found at replication forks, requiredfor DNA replication; also required for Rad53pactivation during DNA replication stress, whereit forms a replication-pausing complex withTof1p and is phosphorylated by Mec1p; proteininvolved in replication checkpoint 0.000650.00425&radic&radic S000000914LSM4YER112WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.000650.00425&radic&radic S000002661MET32YDR253CverifiedZinc-finger DNA-binding protein, involved in regulatingexpression of the methionine biosyntheticgenes, similar to Met31p 0.000650.00425&radic&radic&radic&radic S000001166NDT80YHR124WverifiedMeiosis-specific transcription factor required for exit frompachytene and for full meiotic recombination;activates middle sporulation genes; competeswith Sum1p for binding to promoters containingmiddle sporulation elements (MSE) 0.000650.00425&radic&radic S000002256SNU23YDL098CverifiedComponent of U4/U6.U5 snRNP involved in mRNA splicing viaspliceosome 0.000650.00424&radic&radic S000001764TRM2YKR056WverifiedtRNA methyltransferase, 5-methylates the uridine residue atposition 54 of tRNAs and may also have a rolein tRNA stabilization or maturation; previouslythought to be an endo-exonuclease 0.000650.00423&radic S000000148SAS3YBL052CverifiedHistone acetyltransferase catalytic subunit of NuA3 complexthat acetylates histone H3, involved intranscriptional silencing; homolog of themammalian MOZ proto-oncogene; sas3 gcn5 doublemutation confers lethality 0.000650.00422&radic&radic&radic&radic S000000963SPT2YER161CverifiedProtein involved in negative regulation of transcription;required for RNA polyadenylation; exhibitsregulated interactions with both histones andSWI-SNF components, has similarity to mammalianHMG1 proteins 0.000650.00422&radic&radic S000005251MCK1YNL307CverifiedProtein serine/threonine/tyrosine (dual-specificity) kinaseinvolved in control of chromosome segregationand in regulating entry into meiosis; relatedto mammalian glycogen synthase kinases of theGSK-3 family 0.000650.00422&radic S000005806FSH3YOR280CverifiedSerine hydrolase; sequence is similar to Fsh1p and Fsh2p 0.000650.00422&radic&radic&radic S000000316CYC8YBR112CverifiedGeneral transcriptional co-repressor, acts together with Tup1p;also acts as part of a transcriptionalco-activator complex that recruits the SWI/SNFand SAGA complexes to promoters 0.000650.00422&radic&radic S000002722RAD34YDR314CverifiedProtein involved in nucleotide excision repair (NER);homologous to RAD4 0.000650.00421&radic&radic&radic S000003226MNT2YGL257CverifiedMannosyltransferase involved in adding the 4th and 5th mannoseresidues of O-linked glycans 0.000640.00418&radic&radic S000002591PLP1YDR183WverifiedProtein with a possible role in folding of beta-tubulin; hassimilarity to phosducins, which are GTPaseinhibitors 0.000640.00418&radic&radic S000002161MCD1YDL003WverifiedEssential protein required for sister chromatid cohesion inmitosis and meiosis; subunit of the cohesincomplex; expression is cell cycle regulated andpeaks in S phase 0.000640.00418&radic&radic S000003880JHD2YJR119CuncharacterizedPutative protein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmand nucleus 0.000640.00418&radic&radic S000001346SDS3YIL084CverifiedComponent of the Rpd3p/Sin3p deacetylase complex required forits structural integrity and catalyticactivity, involved in transcriptional silencingand required for sporulation; cells defectivein SDS3 display pleiotropic phenotypes 0.000640.00418&radic&radic&radic&radic S000001250SET5YHR207CverifiedZinc-finger protein of unknown function, contains one canonicaland two unusual fingers in unusualarrangements; deletion enhances replication ofpositive-strand RNA virus 0.000640.00418&radic&radic S000001894SPB4YFL002CverifiedPutative ATP-dependent RNA helicase, nucleolar protein requiredfor synthesis of 60S ribosomal subunits at alate step in the pathway; sediments with 66Spre-ribosomes in sucrose gradients 0.000640.00418&radic&radic S000002360TRM8YDL201WverifiedSubunit of a tRNA methyltransferase complex composed of Trm8pand Trm82p that catalyzes 7-methylguanosinemodification of tRNA 0.000640.00418&radic&radic S000004033TRX1YLR043CverifiedCytoplasmic thioredoxin isoenzyme of the thioredoxin systemwhich protects cells against both oxidative andreductive stress, forms LMA1 complex withPbi2p, acts as a cofactor for Tsa1p, requiredfor ER-Golgi transport and vacuole inheritance 0.000640.00418&radic S000002341LYS20YDL182WverifiedHomocitrate synthase isozyme, catalyzes the condensation ofacetyl-CoA and alpha-ketoglutarate to formhomocitrate, which is the first step in thelysine biosynthesis pathway; highly similar tothe other isozyme, Lys21p 0.000640.00418&radic&radic S000004545DUS1YML080WverifiedDihydrouridine synthase, member of a widespread family ofconserved proteins including Smm1p, Dus3p, andDus4p; modifies pre-tRNA(Phe) at U17 0.000640.00418&radic&radic S000002993PGD1YGL025CverifiedSubunit of the Mediator global transcriptional cofactorcomplex, which is part of the RNA polymerase IIholoenzyme and plays an essential role in basaland activated transcription; direct target ofthe Cyc8p-Tup1p transcriptional corepressor 0.000640.00417&radic&radic&radic&radic S000003712SWI3YJL176CverifiedSubunit of the SWI/SNF chromatin remodeling complex, whichregulates transcription by remodelingchromosomes; required for transcription of manygenes, including ADH1, ADH2, GAL1, HO, INO1 andSUC2 0.000640.00417&radic&radic&radic S000006048HHO1YPL127CverifiedHistone H1, a linker histone required for nucleosome packagingat restricted sites; suppresses DNA repairinvolving homologous recombination; notrequired for telomeric silencing, basaltranscriptional repression, or efficientsporulation 0.000640.00417&radic&radic&radic&radic S000006115DDC1YPL194WverifiedDNA damage checkpoint protein, part of a PCNA-like complexrequired for DNA damage response, required forpachytene checkpoint to inhibit cell cycle inresponse to unrepaired recombinationintermediates; potential Cdc28p substrate 0.000640.00417&radic&radic S000000097ECM15YBL001CverifiedNon-essential protein of unknown function, likely exists astetramer, may be regulated by the binding ofsmall-molecule ligands (possibly sulfate ions),may have a role in yeast cell-wall biogenesis 0.000640.00417&radic&radic&radic S000004466YAP1YML007WverifiedBasic leucine zipper (bZIP) transcription factor required foroxidative stress tolerance; mediatespleiotropic drug and metal resistance;localized to the nucleus in response to thepresence of oxidants 0.000640.00417&radic&radic&radic S000005642RPO31YOR116CverifiedRNA polymerase III subunit C160, part of core enzyme; similarto bacterial beta-prime subunit 0.000640.00417&radic&radic S000006331YPR127WUncharacterizedHypothetical protein 0.000640.00416&radic&radic&radic&radic S000006049TBF1YPL128CverifiedTelobox-containing general regulatory factor; binds to TTAGGGrepeats within subtelomeric anti-silencingregions (STARs) and possibly throughout thegenome and mediates their insulating capacityby blocking silent chromatin propagation 0.000630.00415&radic&radic&radic S000006102CTI6YPL181WverifiedProtein that relieves transcriptional repression by binding tothe Cyc8p-Tup1p corepressor and recruiting theSAGA complex to the repressed promoter;contains a PHD finger domain 0.000630.00414&radic&radic S000003055MMS2YGL087CverifiedProtein involved in error-free postreplication DNA repair;forms a heteromeric complex with Ubc13p thathas a ubiquitin-conjugating activity;cooperates with chromatin-associated RINGfinger proteins, Rad18p and Rad5p 0.000630.00414&radic&radic&radic S000000763RAD23YEL037CverifiedProtein with ubiquitin-like N terminus, recognizes and bindsdamaged DNA (with Rad4p) during nucleotideexcision repair; regulates Rad4p levels,subunit of Nuclear Excision Repair Factor 2(NEF2); homolog of human HR23A and HR23Bproteins 0.000630.00413&radic&radic&radic S000005574RAT1YOR048CverifiedNuclear 5' to 3' single-stranded RNA exonuclease, involved inRNA metabolism, including rRNA and snRNAprocessing as well as mRNA transcriptiontermination 0.000630.00413&radic&radic S000000804NOP16YER002WverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis 0.000630.00413&radic&radic&radic&radic S000000837EDC2YER035WverifiedRNA-binding protein, activates mRNA decapping directly bybinding to the mRNA substrate and enhancing theactivity of the decapping proteins Dcp1p andDcp2p 0.000630.00413&radic&radic&radic S000002214MBP1YDL056WverifiedTranscription factor involved in regulation of cell cycleprogression from G1 to S phase, forms a complexwith Swi6p that binds to MluI cell cycle boxregulatory element in promoters of DNAsynthesis genes 0.000630.00413&radic&radic S000003206HAP2YGL237CverifiedSubunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5pCCAAT-binding complex, a transcriptionalactivator and global regulator of respiratorygene expression; contains sequences sufficientfor both complex assembly and DNA binding 0.000630.00413&radic&radic S000004045SPT8YLR055CverifiedSubunit of the SAGA transcriptional regulatory complex but notpresent in SAGA-like complex SLIK/SALSA,required for SAGA-mediated inhibition at somepromoters 0.000630.00412&radic&radic&radic S000002263NSE4YDL105WverifiedNuclear protein that plays a role in the function of theSmc5p-Rhc18p complex 0.000630.00412&radic&radic S000003393RTS3YGR161CuncharacterizedPutative component of the protein phosphatase type 2A complex 0.000630.00412&radic&radic&radic S000002711RSC3YDR303CverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; essential gene required forregulation of ribosomal protein genes and thecell wall/stress response; highly similar toRsc30p 0.000630.00412&radic&radic&radic S000004645ARG80YMR042WverifiedTranscription factor involved in regulation ofarginine-responsive genes; acts with Arg81p andArg82p 0.000630.00412&radic&radic&radic S000006345KAR3YPR141CverifiedMinus-end-directed microtubule motor that functions in mitosisand meiosis, localizes to the spindle pole bodyand localization is dependent on functionalCik1p, required for nuclear fusion duringmating; potential Cdc28p substrate 0.000630.00411&radic&radic S000005051YAF9YNL107WverifiedSubunit of both the NuA4 histone H4 acetyltransferase complexand the SWR1 complex, may function toantagonize silencing near telomeres; interactsdirectly with Swc4p, has homology to humanleukemogenic protein AF9, contains a YEATSdomain 0.000630.00411&radic&radic S000004581DAT1YML113WverifiedDNA binding protein that recognizes oligo(dA).oligo(dT) tracts;Arg side chain in its N-terminal pentadGly-Arg-Lys-Pro-Gly repeat is required forDNA-binding; not essential for viability 0.000620.0041&radic&radic S000002175CDC7YDL017WverifiedDDK (Dbf4-dependent kinase) catalytic subunit required forfiring origins and replication fork progressionin mitosis through phosphorylation of Mcm2-7pcomplexes and Cdc45p; kinase activitycorrelates with cyclical DBF4 expression 0.000620.0041&radic&radic S000002217RAD59YDL059CverifiedProtein involved in the repair of double-strand breaks in DNAduring vegetative growth via recombination andsingle-strand annealing; anneals complementarysingle-stranded DNA; homologous to Rad52p 0.000620.0041&radic&radic S000006059SPP1YPL138CverifiedSubunit of COMPASS (Set1C), a complex which methylates histoneH3 on lysine 4 and is required in telomerictranscriptional silencing; PHD finger domainprotein similar to human CGBP, an unmethylatedCpG binding protein 0.000620.0041&radic&radic&radic S000002309RPC53YDL150WverifiedRNA polymerase III subunit C53 0.000620.0041&radic&radic S000004224TOP3YLR234WverifiedDNA Topoisomerase III, conserved protein that functions in acomplex with Sgs1p and Rmi1p to relaxsingle-stranded negatively-supercoiled DNApreferentially, involved in telomere stabilityand regulation of mitotic recombination 0.000620.0041&radic&radic&radic S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 0.000620.0041&radic&radic&radic&radic S000002283HNT1YDL125CverifiedAdenosine 5'-monophosphoramidase; interacts physically andgenetically with Kin28p, a CDK and TFIIKsubunit, and genetically with CAK1; member ofthe histidine triad (HIT) superfamily ofnucleotide-binding proteins and similar to Hint 0.000620.00409&radic&radic S000000392NTC20YBR188CverifiedMember of a complex, including Prp19p, that binds to thespliceosome; required for pre-mRNA splicing 0.000620.00409&radic&radic&radic&radic S000002592ATC1YDR184CverifiedNuclear protein, possibly involved in regulation of cationstress responses and/or in the establishment ofbipolar budding pattern 0.000620.00409&radic&radic S000005674SPP2YOR148CverifiedEssential protein that promotes the first step of splicing andis required for the final stages of spliceosomematuration; interacts with Prp2p, which mayrelease Spp2p from the spliceosome followingthe first cleavage reaction 0.000620.00408&radic&radic&radic S000002510STE5YDR103WverifiedPheromone-responsive scaffold protein; shuttles from thenucleus to the plasma membrane and assembleskinases Ste11p, Ste7p, and Fus3p into aspecific signaling complex; active oligomericform interacts with Ste4p-Ste18p complex 0.000620.00408&radic&radic&radic S000001129YHR087WVerifiedProtein involved in RNA metabolism, one of two yeast homologs (with Sdo1p/Ylr022cp) of the human protein SBDS responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome, also conserved in Archaea 0.000620.00408&radic&radic S000002329UGA3YDL170WverifiedTranscriptional activator necessary for gamma-aminobutyrate(GABA)-dependent induction of GABA genes (suchas UGA1, UGA2, UGA4); zinc-finger transcriptionfactor of the Zn(2)-Cys(6) binuclear clusterdomain type; localized to the nucleus 0.000620.00408&radic&radic S000001504MAK11YKL021CverifiedProtein essential for cell growth and replication of M dsRNAvirus; contains four beta-transducin repeats 0.000620.00408&radic&radic&radic S000003436ADE3YGR204WverifiedCytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase,involved in single carbon metabolism andrequired for biosynthesis of purines,thymidylate, methionine, and histidine 0.000620.00408&radic&radic S000005413DDR2YOL052C-AverifiedMultistress response protein, expression is activated by avariety of xenobiotic agents and environmentalor physiological stresses 0.000620.00408 S000001112TRM5YHR070WverifiedtRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacentto the anticodon that has a role in preventionof frameshifting; highly conserved acrossArchaea, Bacteria, and Eukarya 0.000620.00408&radic S000004134ACF2YLR144CverifiedIntracellular beta-1,3-endoglucanase, expression is inducedduring sporulation; may have a role in corticalactin cytoskeleton assembly 0.000620.00407&radic&radic&radic&radic S000002574TAF10YDR167WverifiedSubunit (145 kDa) of TFIID and SAGA complexes, involved in RNApolymerase II transcription initiation and inchromatin modification 0.000620.00407&radic&radic&radic S000003389CHO2YGR157WverifiedPhosphatidylethanolamine methyltransferase (PEMT), catalyzesthe first step in the conversion ofphosphatidylethanolamine to phosphatidylcholineduring the methylation pathway ofphosphatidylcholine biosynthesis 0.000620.00407&radic&radic S000004966HDA1YNL021WverifiedPutative catalytic subunit of a class II histone deacetylasecomplex that also contains Hda2p and Hda3p;Hda1p interacts with the Hda2p-Hda3p subcomplexto form an active tetramer; deletion increaseshistone H2B, H3 and H4 acetylation 0.000610.00407&radic&radic S000005943RAD1YPL022WverifiedSingle-stranded DNA endonuclease (with Rad10p), cleavessingle-stranded DNA during nucleotide excisionrepair and double-strand break repair; subunitof Nucleotide Excision Repair Factor 1 (NEF1);homolog of human XPF protein 0.000610.00407&radic&radic&radic S000005046POL1YNL102WverifiedCatalytic subunit of the DNA polymerase alpha-primase complex,required for the initiation of DNA replicationduring mitotic DNA synthesis and premeiotic DNAsynthesis 0.000610.00407&radic&radic S000004187SIK1YLR197WverifiedEssential evolutionarily-conserved nucleolar protein componentof the box C/D snoRNP complexes that direct2'-O-methylation of pre-rRNA during itsmaturation; overexpression causes spindleorientation defects 0.000610.00407&radic&radic&radic&radic S000004058FYV7YLR068WverifiedProtein of unknown function, required for survival uponexposure to K1 killer toxin; involved inprocessing the 35S rRNA primary transcript togenerate the 20S and 27SA2 pre-rRNA transcripts 0.000610.00407&radic&radic&radic S000001845SWP82YFL049WuncharacterizedMember of the SWI/SNF chromatin remodeling complex in which itplays an as yet unidentified role; hasidentifiable counterparts in closely relatedyeast species; abundantly expressed in manygrowth conditions; paralog of Npl6p 0.000610.00407&radic&radic S000000112FUS3YBL016WverifiedMitogen-activated protein kinase involved in mating pheromoneresponse; activated by phosphorylation bySte7p; provides specificity during the matingvs. filamentous growth response byphosphorylating transcriptional and cytoplasmictargets 0.000610.00407&radic&radic S000005080EAF7YNL136WverifiedSubunit of the NuA4 histone acetyltransferase complex, whichacetylates the N-terminal tails of histones H4and H2A 0.000610.00407&radic&radic S000000836YER034WUncharacterizedHypothetical protein 0.000610.00407&radic&radic&radic S000003269ACB1YGR037CverifiedAcyl-CoA-binding protein, transports newly synthesized acyl-CoAesters from fatty acid synthetase (Fas1p-Fas2p)to acyl-CoA-consuming processes 0.000610.00407&radic S000001869BST1YFL025CverifiedGPI inositol deacylase of the ER that negatively regulatesCOPII vesicle formation, prevents production ofvesicles with defective subunits, required forproper discrimination between resident ERproteins and Golgi-bound cargo molecules 0.000610.00407&radic&radic S000000398SOY1YBR194WuncharacterizedProtein proposed to be associated with the nuclear pore complex 0.000610.00407&radic&radic&radic&radic S000000808NUG1YER006WverifiedGTPase that associates with nuclear 60S pre-ribosomes, requiredfor export of 60S ribosomal subunits from thenucleus 0.000610.00407&radic&radic&radic&radic S000002772CDC40YDR364CverifiedPre-mRNA splicing factor, important for catalytic step II ofpre-mRNA splicing and plays a role in cellcycle progression; required for DNA synthesisduring mitosis and meiosis; has WD repeats 0.000610.00406&radic&radic&radic S000000189ROX3YBL093CverifiedRNA polymerase II holoenzyme component 0.000610.00406&radic&radic&radic S000002552TAF12YDR145WverifiedSubunit (61/68 kDa) of TFIID and SAGA complexes, involved inRNA polymerase II transcription initiation andin chromatin modification, similar to histoneH2A 0.000610.00406&radic&radic&radic&radic S000006366ORC4YPR162CverifiedSubunit of the origin recognition complex, which directs DNAreplication by binding to replication originsand is also involved in transcriptionalsilencing 0.000610.00406&radic S000003510CWC22YGR278WverifiedEssential protein, component of a complex containing Cef1p, hassimilarity to S. pombe Cwf22p; putativespliceosomal component, based on computationalanalysis of large-scale protein-proteininteraction data 0.000610.00406&radic&radic&radic&radic S000001943BNA6YFR047CverifiedQuinolinate phosphoribosyl transferase, required forbiosynthesis of nicotinic acid from tryptophanvia kynurenine pathway 0.000610.00406&radic S000002429CIS1YDR022CverifiedProtein of unknown function that may be involved in microtubuleorganization; high-copy suppressor of CIK1deletion 0.000610.00405&radic S000002977LEU1YGL009CverifiedIsopropylmalate isomerase, catalyzes the second step in theleucine biosynthesis pathway 0.000610.00405&radic&radic S000001722YPT52YKR014CverifiedGTPase, similar to Ypt51p and Ypt53p and to mammalian rab5;required for vacuolar protein sorting andendocytosis 0.000610.00405&radic&radic S000002499UBC13YDR092WverifiedUbiquitin-conjugating enzyme involved in the error-free DNApostreplication repair pathway; interacts withMms2p to assemble ubiquitin chains at the UbLys-63 residue; DNA damage triggersredistribution from the cytoplasm to thenucleus 0.000610.00405&radic S000000698YCR101CUncharacterizedPutative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene 0.000610.00405&radic&radic S000006074RAD53YPL153CverifiedProtein kinase, required for cell-cycle arrest in response toDNA damage; activated by transautophosphorylation when interacting withhyperphosphorylated Rad9p; also interacts withARS1 and plays a role in initiation of DNAreplication 0.000610.00405&radic&radic&radic S000004248GSY2YLR258WverifiedGlycogen synthase, similar to Gsy1p; expression induced byglucose limitation, nitrogen starvation, heatshock, and stationary phase; activity regulatedby cAMP-dependent, Snf1p and Pho85p kinases aswell as by the Gac1p-Glc7p phosphatase 0.000610.00405&radic S000000792HPA3YEL066WverifiedD-Amino acid N-acetyltransferase, catalyzes N-acetylation ofD-amino acids through ordered bi-bi mechanismin which acetyl-CoA is first substrate boundand CoA is last product liberated; similar toHpa2p, acetylates histones weakly in vitro 0.000610.00405&radic&radic&radic S000006129RKM1YPL208WverifiedSET-domain lysine-N-methyltransferase, catalyzes the formationof dimethyllysine residues on the largeribsomal subunit protein L23a (RPL23A andRPL23B) 0.000610.00404&radic&radic&radic S000005437BRX1YOL077CverifiedNucleolar protein, constituent of 66S pre-ribosomal particles;depletion leads to defects in rRNA processingand a block in the assembly of large ribosomalsubunits; possesses a sigma(70)-likeRNA-binding motif 0.000610.00404&radic&radic S000003825CCT5YJR064WverifiedSubunit of the cytosolic chaperonin Cct ring complex, relatedto Tcp1p, required for the assembly of actinand tubulins in vivo 0.000610.00404 S000002458YDR051CUncharacterizedHypothetical protein 0.000610.00404&radic S000002667YAP6YDR259CverifiedPutative basic leucine zipper (bZIP) transcription factor;overexpression increases sodium and lithiumtolerance 0.000610.00404&radic&radic&radic S000001858RPO41YFL036WverifiedMitochondrial RNA polymerase; single subunit enzyme similar tothose of T3 and T7 bacteriophages; requires aspecificity subunit encoded by MTF1 forpromoter recognition 0.000610.00404&radic&radic S000003908HMS2YJR147WverifiedProtein with similarity to heat shock transcription factors;overexpression suppresses the pseudohyphalfilamentation defect of a diploid mep1 mep2homozygous null mutant 0.000610.00404&radic&radic S000004833ERG8YMR220WverifiedPhosphomevalonate kinase, an essential cytosolic enzyme thatacts in the biosynthesis of isoprenoids andsterols, including ergosterol, from mevalonate 0.000610.00404&radic&radic&radic S000005313ALG12YNR030WverifiedAlpha-1,6-mannosyltransferase localized to the ER; responsiblefor the addition of the alpha-1,6 mannose todolichol-linked Man7GlcNAc2, acts in thedolichol pathway for N-glycosylation 0.00060.00404&radic S000003290YGR058WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 0.00060.00404&radic S000000253REB1YBR049CverifiedRNA polymerase I enhancer binding protein; DNA binding proteinwhich binds to genes transcribed by both RNApolymerase I and RNA polymerase II; requiredfor termination of RNA polymerase Itranscription 0.00060.00404&radic&radic S000002738UBX5YDR330WuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p 0.00060.00403&radic&radic&radic S000002632HTB1YDR224CverifiedOne of two nearly identical (see HTB2) histone H2B subtypesrequired for chromatin assembly and chromosomefunction; Rad6p-Bre1p-Lge1p mediatedubiquitination regulates transcriptionalactivation, meiotic DSB formation and H3methylation 0.00060.00403&radic&radic S000005130UBP10YNL186WverifiedUbiquitin-specific protease that deubiquitinatesubiquitin-protein moieties; may regulatesilencing by acting on Sir4p; involved inposttranscriptionally regulating Gap1p andpossibly other transporters; primarily locatedin the nucleus 0.00060.00403&radic&radic S000003760YJL225CUncharacterizedPutative protein of unknown function; similarity to DNA helicases that are also encoded within subtelomeric Y' elements and induced in telomerase deficient survivors 0.00060.00403&radic S000001887BLM10YFL007WverifiedProteosome activator subunit; found in association with coreparticles, with and without the 19S regulatoryparticle; required for resistance to bleomycin,may be involved in protecting against oxidativedamage; similar to mammalian PA200 0.00060.00403&radic&radic&radic S000002718SUM1YDR310CverifiedTranscriptional repressor required for mitotic repression ofmiddle sporulation-specific genes; involved intelomere maintenance, regulated by thepachytene checkpoint 0.00060.00403&radic&radic&radic S000003896MCM22YJR135CverifiedProtein involved in minichromosome maintenance; component ofthe kinetochore; binds to centromeric DNA in aCtf19p-dependent manner 0.00060.00403&radic&radic S000000750RIP1YEL024WverifiedUbiquinol-cytochrome-c reductase, a Rieske iron-sulfur proteinof the mitochondrial cytochrome bc1 complex;transfers electrons from ubiquinol tocytochrome c1 during respiration 0.00060.00403&radic&radic S000001001YAP3YHL009CverifiedBasic leucine zipper (bZIP) transcription factor 0.00060.00403&radic&radic&radic S000001078BRL1YHR036WverifiedEssential nuclear envelope integral membrane protein identifiedas a suppressor of a cold-sensitive mutant ofCRM1, a karyopherin; homologous to andinteracts with Brr6p, which is a nuclearenvelope protein involved in nuclear export 0.00060.00402&radic&radic S000000745MMS21YEL019CverifiedSUMO ligase involved in chromosomal organization and DNArepair; essential subunit of theMms21-Smc5-Smc6 complex; mutants are sensitiveto methyl methanesulfonate and show increasedspontaneous mutation and mitotic recombination 0.00060.00402&radic&radic&radic S000005551HST3YOR025WverifiedMember of the Sir2 family of NAD(+)-dependent proteindeacetylases; involved along with Hst4p intelomeric silencing, cell cycle progression,radiation resistance, genomic stability andshort-chain fatty acid metabolism 0.00060.00402&radic&radic&radic S000000912KAP123YER110CverifiedKaryopherin beta, mediates nuclear import of ribosomal proteinsprior to assembly into ribosomes and import ofhistones H3 and H4; localizes to the nuclearpore, nucleus, and cytoplasm; exhibits geneticinteractions with RAI1 0.00060.00402&radic&radic S000003992NOC3YLR002CverifiedProtein that forms a nuclear complex with Noc2p that binds to66S ribosomal precursors to mediate theirintranuclear transport; also binds to chromatinto promote the association of DNA replicationfactors and replication initiation 0.00060.00402&radic&radic&radic S000005242CLA4YNL298WverifiedCdc42p activated signal transducing kinase of the PAK(p21-activated kinase) family, involved inseptin ring assembly and cytokinesis; directlyphosphorylates septins Cdc3p and Cdc10p; otheryeast PAK family members are Ste20p and Skm1p 0.000590.00401&radic&radic S000001498PUT3YKL015WverifiedTranscriptional activator of proline utilization genes,constitutively binds PUT1 and PUT2 promotersequences and undergoes a conformational changeto form the active state; has a Zn(2)-Cys(6)binuclear cluster domain 0.00060.00401&radic&radic&radic S000002981PDR1YGL013CverifiedZinc cluster protein that is a master regulator involved inrecruiting other zinc cluster proteins topleiotropic drug response elements (PDREs) tofine tune the regulation of multidrugresistance genes 0.00060.00401&radic&radic&radic S000006014NOG1YPL093WverifiedPutative GTPase that associates with free 60S ribosomalsubunits in the nucleolus and is required for60S ribosomal subunit biogenesis; constituentof 66S pre-ribosomal particles; member of theODN family of nucleolar G-proteins 0.000590.00401&radic&radic&radic S000003942KNS1YLL019CverifiedNonessential putative protein kinase of unknown cellular role;member of the LAMMER family of protein kinases,which are serine/threonine kinases also capableof phosphorylating tyrosine residues 0.00060.00401&radic&radic&radic S000002723IPK1YDR315CverifiedInositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear proteinrequired for synthesis of1,2,3,4,5,6-hexakisphosphate (phytate), whichis integral to cell function; has 2 motifsconserved in other fungi; ipk1 gle1 doublemutant is inviable 0.000590.00401&radic S000001481YIR042CUncharacterizedPutative protein of unknown function; YIR042C is a non-essential gene 0.000590.00401 S000004872YMR259CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR259C is not an essential gene 0.00060.00401&radic&radic&radic S000006386SMX3YPR182WverifiedCore Sm protein Sm F; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm F 0.00060.00401&radic&radic&radic S000000925YCK3YER123WverifiedPalmitoylated, vacuolar membrane-localized casein kinase Iisoform; negatively regulates vacuole fusionduring hypertonic stress via phosphorylation ofthe HOPS complex subunit, Vps41p; sharesoverlapping essential functions with Hrr25p 0.00060.00401&radic S000005092ALF1YNL148CverifiedAlpha-tubulin folding protein, similar to mammalian cofactor B;Alf1p-GFP localizes to cytoplasmicmicrotubules; required for the folding ofalpha-tubulin and may play an additional rolein microtubule maintenance 0.00060.00401&radic&radic&radic S000000521DCC1YCL016CverifiedSubunit of a complex with Ctf8p and Ctf18p that shares somecomponents with Replication Factor C, requiredfor sister chromatid cohesion and telomerelength maintenance 0.00060.00401&radic&radic S000001897LOC1YFR001WverifiedNuclear protein involved in asymmetric localization of ASH1mRNA; binds double-stranded RNA in vitro;constituent of 66S pre-ribosomal particles 0.00060.00401&radic&radic&radic&radic S000001012OPI1YHL020CverifiedTranscriptional regulator of a variety of genes;phosphorylation by protein kinase A stimulatesOpi1p function in negative regulation ofphospholipid biosynthetic genes; involved intelomere maintenance 0.00060.00401&radic&radic&radic S000004840TAF7YMR227CverifiedTFIID subunit (67 kDa), involved in RNA polymerase IItranscription initiation 0.000590.004&radic&radic&radic S000004080XDJ1YLR090WverifiedPutative chaperone, homolog of E. coli DnaJ, closely related toYdj1p; the authentic, non-tagged protein isdetected in highly purified mitochondria inhigh-throughput studies 0.000590.004&radic&radic&radic S000000270NRG2YBR066CverifiedTranscriptional repressor that mediates glucose repression andnegatively regulates filamentous growth; hassimilarity to Nrg1p 0.000590.004&radic&radic S000002580ARG82YDR173CverifiedInositol polyphosphate multikinase (IPMK), sequentiallyphosphorylates Ins(1,4,5)P3 to formIns(1,3,4,5,6)P5; putative nuclear lipidinositol PI3-kinase; regulates arginine-,phosphate-, and nitrogen-responsive genes 0.000590.004&radic&radic&radic&radic S000002797SAC7YDR389WverifiedGTPase activating protein (GAP) for Rho1p, involved insignaling to the actin cytoskeleton, nullmutations suppress tor2 mutations andtemperature sensitive mutations in actin;potential Cdc28p substrate 0.000590.004&radic&radic&radic S000000534BIK1YCL029CverifiedMicrotubule-associated protein, component of the interfacebetween microtubules and kinetochore, involvedin sister chromatid separation; essential inpolyploid cells but not in haploid or diploidcells; ortholog of mammalian CLIP-170 0.000590.004&radic&radic&radic S000003931DRS1YLL008WverifiedNucleolar DEAD-box protein required for ribosome assembly andfunction, including synthesis of 60S ribosomalsubunits; constituent of 66S pre-ribosomalparticles 0.000590.004&radic&radic&radic S000000162SEF1YBL066CverifiedPutative transcription factor, has homolog in Kluyveromyceslactis 0.000590.004&radic&radic S000004122YLR132CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to mitochondria and nucleus; YLR132C is an essential gene 0.000590.004&radic S000004777MLH1YMR167WverifiedProtein required for mismatch repair in mitosis and meiosis,postmeiotic segregation, and spore viability;forms a complex with Pms1p and Msh2p to repairmismatched DNA; human homolog is associatedwith hereditary non-polyposis colon cancer 0.000590.00399&radic&radic&radic S000005154MER1YNL210WverifiedProtein with RNA-binding motifs required for meiosis-specificmRNA splicing; required for chromosome pairingand meiotic recombination 0.000590.00399&radic&radic&radic S000005898GPB1YOR371CverifiedProposed beta subunit of the heterotrimeric G protein;interacts with the receptor Grp1p, affectssignaling by altering the level ofphosphorylation of PKS substrates; regulatespseudohyphal growth through cAMP levels;homolog of Gpb2p 0.000590.00399&radic S000004980YNL035CUncharacterizedPutative protein of unknown function with similarity to proteins containing WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene 0.000590.00399&radic&radic S000001632DBR1YKL149CverifiedRNA lariat debranching enzyme, involved in intron turnover;required for efficient Ty1 transposition 0.000590.00399&radic&radic&radic&radic S000003556MPS3YJL019WverifiedEssential integral membrane protein required for spindle polebody duplication and for nuclear fusion,localizes to the spindle pole body half bridge,interacts with DnaJ-like chaperone Jem1p andwith centrin homolog Cdc31p 0.000590.00399&radic S000005989YPL068CUncharacterizedHypothetical protein 0.000590.00399&radic&radic S000000842GLN3YER040WverifiedTranscriptional activator of genes regulated by nitrogencatabolite repression (NCR), localization andactivity regulated by quality of nitrogensource 0.000590.00399&radic&radic&radic&radic S000003341CLB6YGR109CverifiedB-type cyclin involved in DNA replication during S phase;activates Cdc28p to promote initiation of DNAsynthesis; functions in formation of mitoticspindles along with Clb3p and Clb4p; mostabundant during late G1 0.000590.00399&radic&radic&radic S000006090MEX67YPL169CverifiedPoly(A)RNA binding protein involved in nuclear mRNA export,component of the nuclear pore; ortholog ofhuman TAP 0.000590.00399&radic S000002713HNT2YDR305CverifiedDinucleoside triphosphate hydrolase; has similarity to thetumor suppressor FHIT and belongs to thehistidine triad (HIT) superfamily ofnucleotide-binding proteins 0.000590.00399&radic&radic S000000406CDC47YBR202WverifiedComponent of the hexameric MCM complex, which is important forpriming origins of DNA replication in G1 andbecomes an active ATP-dependent helicase thatpromotes DNA melting and elongation whenactivated by Cdc7p-Dbf4p in S-phase 0.000590.00398&radic&radic&radic&radic S000005582NOB1YOR056CverifiedEssential nuclear protein involved in proteasome maturation andsynthesis of 40S ribosomal subunits; requiredfor cleavage of the 20S pre-rRNA to generatethe mature 18S rRNA 0.000590.00398&radic&radic&radic S000000869YER067WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YER067W is not an essential gene 0.000590.00398&radic&radic S000005125YNL181WUncharacterizedProtein required for cell viability 0.000590.00398&radic S000004897YKU70YMR284WverifiedSubunit of the telomeric Ku complex (Yku70p-Yku80p), involvedin telomere length maintenance, structure andtelomere position effect; relocates to sites ofdouble-strand cleavage to promote nonhomologousend joining during DSB repair 0.000590.00398&radic&radic&radic S000002490RRP8YDR083WverifiedNucleolar protein involved in rRNA processing, pre-rRNAcleavage at site A2; also involved in telomeremaintenance; mutation is synthetically lethalwith a gar1 mutation 0.000590.00398&radic&radic&radic S000005688YRR1YOR162CverifiedZn2-Cys6 zinc-finger transcription factor that activates genesinvolved in multidrug resistance; paralog ofYrm1p, acting on an overlapping set of targetgenes 0.000590.00398&radic&radic&radic S000001017SNF6YHL025WverifiedSubunit of the SWI/SNF chromatin remodeling complex involved intranscriptional regulation; functionsinterdependently in transcriptional activationwith Snf2p and Snf5p 0.000590.00398&radic&radic S000002599HST4YDR191WverifiedMember of the Sir2 family of NAD(+)-dependent proteindeacetylases; involved along with Hst3p insilencing at telomeres, cell cycle progression,radiation resistance, genomic stability andshort-chain fatty acid metabolism 0.000590.00398&radic&radic&radic&radic S000002671DIN7YDR263CverifiedMitochondrial nuclease functioning in DNA repair andreplication, modulates the stability of themitochondrial genome, induced by exposure tomutagens, also induced during meiosis at a timenearly coincident with commitment torecombination 0.000590.00398&radic S000005609WHI5YOR083WverifiedRepressor of G1 transcription that binds to SCB binding factor(SBF) at SCB target promoters in early G1;phosphorylation of Whi5p by the CDK,Cln3p/Cdc28p relieves repression and promoterbinding by Whi5; periodically expressed in G1 0.000590.00398&radic&radic&radic S000001595ABF1YKL112WverifiedDNA binding protein with possible chromatin-reorganizingactivity involved in transcriptionalactivation, gene silencing, and DNA replicationand repair 0.000590.00398&radic&radic&radic S000005643RPT5YOR117WverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; recruited to theGAL1-10 promoter region upon induction oftranscription 0.000590.00398&radic&radic S000000687KIN82YCR091WverifiedPutative serine/threonine protein kinase, most similar tocyclic nucleotide-dependent protein kinasesubfamily and the protein kinase C subfamily 0.000590.00398&radic S000005162MGS1YNL218WverifiedProtein with DNA-dependent ATPase and ssDNA annealingactivities involved in maintenance of genome;interacts functionally with DNA polymerasedelta; homolog of human Werner helicaseinteracting protein (WHIP) 0.000590.00398&radic&radic&radic S000006046KAP120YPL125WverifiedKaryopherin with a role in the assembly or export of 60Sribosomal subunits 0.000590.00398&radic&radic&radic S000000958YER156CUncharacterizedHypothetical protein 0.000590.00398&radic&radic S000003058LIF1YGL090WverifiedProtein involved in DNA double-strand break repair; physicallyinteracts with DNA ligase 4 (Lig4p); homologousto mammalian XRCC4 protein 0.000590.00398&radic&radic&radic S000006026YPL105CUncharacterizedHypothetical protein 0.000580.00396&radic&radic&radic S000001721PRY2YKR013WverifiedProtein of unknown function, has similarity to Pry1p and Pry3pand to the plant PR-1 class of pathogen relatedproteins 0.000580.00396&radic S000001676SDS22YKL193CverifiedConserved nuclear regulatory subunit of Glc7p type 1 proteinserine-threonine phosphatase (PP1), functionspositively with Glc7p to promotedephosphorylation of nuclear substratesrequired for chromosome transmission duringmitosis 0.000580.00396&radic&radic&radic S000001947RET2YFR051CverifiedDelta subunit of the coatomer complex (COPI), which coatsGolgi-derived transport vesicles; involved inretrograde transport between Golgi and ER 0.000580.00396&radic S000000613PET18YCR020CverifiedProtein required for respiratory growth and stability of themitochondrial genome 0.000580.00396&radic&radic S000001733RPC37YKR025WverifiedRNA polymerase III subunit C37 0.000580.00396&radic&radic&radic S000004629YMR027WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene 0.000580.00396&radic S000004534POB3YML069WverifiedSubunit of the heterodimeric FACT complex (Spt16p-Pob3p), whichfacilitates RNA Polymerase II transcriptionelongation through nucleosomes by destabilizingand then reassembling nucleosome structure 0.000580.00396&radic&radic S000005420GPD2YOL059WverifiedNAD-dependent glycerol 3-phosphate dehydrogenase, homolog ofGpd1p, expression is controlled by anoxygen-independent signaling pathway requiredto regulate metabolism under anoxic conditions;located in cytosol and mitochondria 0.000590.00396&radic&radic&radic S000005163ALG9YNL219CverifiedMannosyltransferase, involved in N-linked glycosylation;catalyzes the transfer of mannose fromDol-P-Man to lipid-linked oligosaccharides;mutation of the human ortholog causes type 1congenital disorders of glycosylation 0.000580.00396&radic&radic&radic S000004261YLR271WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YLR271W is not an essential gene 0.000580.00396&radic&radic S000004518CYB2YML054CverifiedCytochrome b2 (L-lactate cytochrome-c oxidoreductase),component of the mitochondrial intermembranespace, required for lactate utilization;expression is repressed by glucose andanaerobic conditions 0.000580.00396&radic S000001462DAL81YIR023WverifiedPositive regulator of genes in multiple nitrogen degradationpathways; contains DNA binding domain but doesnot appear to bind the dodecanucleotidesequence present in the promoter region of manygenes involved in allantoin catabolism 0.000580.00396&radic&radic S000006164SRP68YPL243WverifiedCore component of the signal recognition particle (SRP)ribonucleoprotein (RNP) complex that functionsin targeting nascent secretory proteins to theendoplasmic reticulum (ER) membrane 0.000580.00396&radic&radic S000002703HDA2YDR295CverifiedSubunit of a possibly tetrameric trichostatin A-sensitive classII histone deacetylase complex containing anHda1p homodimer and an Hda2p-Hda3p heterodimer;involved in telomere maintenance 0.000580.00395&radic&radic&radic&radic S000003233YGR001CUncharacterizedPutative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae 0.000580.00395&radic&radic S000004088CHA4YLR098CverifiedDNA binding transcriptional activator, mediatesserine/threonine activation of the catabolicL-serine (L-threonine) deaminase (CHA1);Zinc-finger protein with Zn[2]-Cys[6]fungal-type binuclear cluster domain 0.000580.00395&radic&radic&radic&radic S000001610PGM1YKL127WverifiedPhosphoglucomutase, minor isoform; catalyzes the conversionfrom glucose-1-phosphate toglucose-6-phosphate, which is a key step inhexose metabolism 0.000580.00395&radic&radic S000005379YOL019WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole 0.000580.00395&radic&radic S000002805NCB2YDR397CverifiedBeta subunit of the NC2 dimeric histone-fold complex; repressesRNA polymerase II transcription through bindingto TBP and inhibition of TFIIA and TFIIB;homologous to the Dr1 subunit of the mammalianNC2 (negative cofactor2) 0.000580.00395&radic&radic&radic S000005805RFM1YOR279CverifiedDNA-binding protein required for vegetative repression ofmiddle sporulation genes; specificity factorthat directs the Hst1p histone deacetylase tosome of the promoters regulated by Sum1p;involved in telomere maintenance 0.000580.00395&radic&radic S000005415PSH1YOL054WuncharacterizedNuclear protein, putative RNA polymerase II elongation factor;isolated as Pob3p/Spt16p-binding protein 0.000580.00395&radic&radic S000001478YPS6YIR039CverifiedPutative GPI-anchored aspartic protease 0.000580.00395 S000003866ADO1YJR105WverifiedAdenosine kinase, required for the utilization ofS-adenosylmethionine (AdoMet); may be involvedin recycling adenosine produced through themethyl cycle 0.000580.00395&radic S000000104HIR1YBL008WverifiedNon-essential transcriptional corepressor involved in the cellcycle-regulated transcription of histone H2A,H2B, H3 and H4 genes; contributes to nucleosomeformation, heterochromatic gene silencing, andformation of functional kinetochores 0.000580.00395&radic S000005700MED4YOR174WverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation 0.000580.00395&radic S000000451ENP1YBR247CverifiedProtein associated with U3 and U14 snoRNAs, required forpre-rRNA processing and 40S ribosomal subunitsynthesis; localized in the nucleus andconcentrated in the nucleolus 0.000580.00395&radic&radic&radic&radic&radic S000001571YKL088WUncharacterizedPredicted phosphopantothenoylcysteine decarboxylase, may be involved in coenzyme A biosynthesis; interacts with Sis2p and Vhs3p 0.000580.00395 S000002775YDR367WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 0.000580.00395&radic&radic S000004778CEP3YMR168CverifiedEssential kinetochore protein, component of the CBF3 complexthat binds the CDEIII region of the centromere;contains an N-terminal Zn2Cys6 type zinc fingerdomain, a C-terminal acidic domain, and aputative coiled coil dimerization domain 0.000580.00395&radic&radic S000000215IPP1YBR011CverifiedCytoplasmic inorganic pyrophosphatase (PPase), catalyzes therapid exchange of oxygens from Pi with water,highly expressed and essential for viability,active-site residues show identity to thosefrom E. coli PPase 0.000580.00395&radic S000001783YKR075CUncharacterizedProtein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p 0.000580.00395&radic&radic&radic S000002303YDL144CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene. 0.000580.00395&radic&radic S000000441PRP5YBR237WverifiedRNA helicase in the DEAD-box family, necessary forprespliceosome formation, bridges U1 and U2snRNPs and enables stable U2 snRNP associationwith intron RNA 0.000580.00395&radic&radic S000001325YRB2YIL063CverifiedProtein of unknown function involved in nuclear processes ofthe Ran-GTPase cycle; involved in nuclearprotein export; contains Ran Binding Domain andFxFG repeats; interacts with Srm1p, GTP-Gsp1p,Rna1p and Crm1p; is not essential 0.000580.00395&radic&radic S000004774MSS11YMR164CverifiedTranscription factor involved in regulation of invasive growthand starch degradation; controls the activationof MUC1 and STA2 in response to nutritionalsignals 0.000580.00395&radic&radic S000005885HAP5YOR358WverifiedSubunit of the heme-activated, glucose-repressed Hap2/3/4/5CCAAT-binding complex, a transcriptionalactivator and global regulator of respiratorygene expression; required for assembly and DNAbinding activity of the complex 0.000580.00395&radic&radic&radic&radic S000001458MUC1YIR019CverifiedGPI-anchored cell surface glycoprotein required for diploidpseudohyphal formation and haploid invasivegrowth, transcriptionally regulated by the MAPKpathway (via Ste12p and Tec1p) and the cAMPpathway (via Flo8p) 0.000580.00395&radic&radic S000001222OYE2YHR179WverifiedWidely conserved NADPH oxidoreductase containing flavinmononucleotide (FMN), homologous to Oye3p withslight differences in ligand binding andcatalytic properties; may be involved in sterolmetabolism 0.000580.00395&radic&radic&radic S000000203YBL107CUncharacterizedHypothetical protein 0.000580.00395&radic S000005021APJ1YNL077WverifiedPutative chaperone of the HSP40 (DNAJ) family; overexpressioninterferes with propagation of the [Psi+]prion; the authentic, non-tagged protein isdetected in highly purified mitochondria inhigh-throughput studies 0.000580.00394&radic&radic S000004173TOS4YLR183CverifiedTranscription factor that binds to a number of promoterregions, particularly promoters of some genesinvolved in pheromone response and cell cycle;potential Cdc28p substrate; expression isinduced in G1 by bound SBF 0.000580.00394&radic&radic S000005184NAR1YNL240CverifiedNuclear architecture related protein; component of thecytosolic iron-sulfur (FeS) protein assemblymachinery, required for maturation of cytosolicand nuclear FeS proteins; homologous to humanNarf 0.000580.00394&radic&radic S000006032CAR1YPL111WverifiedArginase, responsible for arginine degradation, expressionresponds to both induction by arginine andnitrogen catabolite repression; disruptionenhances freeze tolerance 0.000580.00394&radic&radic&radic S000003520MAL13YGR288WverifiedMAL-activator protein, part of complex locus MAL1;nonfunctional in genomic reference strain S288C 0.000580.00394&radic&radic&radic S000003162HOS2YGL194CverifiedHistone deacetylase required for gene activation via specificdeacetylation of lysines in H3 and H4 histonetails; subunit of the Set3 complex, ameiotic-specific repressor of sporulationspecific genes that contains deacetylaseactivity 0.000580.00394&radic&radic&radic&radic S000000457SRB6YBR253WverifiedEssentital subunit of the RNA polymerase II mediator complex;associates with core polymerase subunits toform the RNA polymerase II holoenzyme;essential for transcriptional regulation 0.000580.00394&radic&radic&radic S000006066KES1YPL145CverifiedMember of the oxysterol binding protein family, which includesseven yeast homologs; involved in negativeregulation of Sec14p-dependent Golgi complexsecretory functions, peripheral membraneprotein that localizes to the Golgi complex 0.000580.00394&radic&radic&radic S000003008HEM2YGL040CverifiedDelta-aminolevulinate dehydratase, a homo-octameric enzyme,catalyzes the conversion ofdelta-aminolevulinic acid to porphobilinogen,the second step in the heme biosyntheticpathway; localizes to both the cytoplasm andnucleus 0.000580.00394&radic&radic&radic S000001699URA1YKL216WverifiedDihydroorotate dehydrogenase, catalyzes the fourth enzymaticstep in the de novo biosynthesis ofpyrimidines, converting dihydroorotic acid intoorotic acid 0.000580.00394&radic&radic&radic S000001210THP2YHR167WverifiedSubunit of the THO complex, which connects transcriptionelongation and mitotic recombination, and ofthe TREX complex, which is recruited toactivated genes and couples transcription tomRNA export; involved in telomere maintenance 0.000580.00394&radic&radic&radic S000001914YFR018CUncharacterizedHypothetical protein 0.000580.00394&radic&radic S000004377SWC7YLR385CverifiedProtein of unknown function, component of the Swr1p complexthat incorporates Htz1p into chromatin 0.000580.00394&radic&radic&radic S000002289LYS21YDL131WverifiedHomocitrate synthase isozyme, catalyzes the condensation ofacetyl-CoA and alpha-ketoglutarate to formhomocitrate, which is the first step in thelysine biosynthesis pathway; highly similar tothe other isozyme, Lys20p 0.000580.00394&radic&radic S000002777XRS2YDR369CverifiedProtein required for DNA repair; component of the Mre11complex, which is involved in double strandbreaks, meiotic recombination, telomeremaintenance, and checkpoint signaling 0.000570.00393&radic&radic&radic&radic S000005174ELA1YNL230CverifiedElongin A, F-box protein that forms a heterodimer with Elc1pand participates in transcription elongation 0.000570.00393&radic&radic&radic&radic S000003690VPS35YJL154CverifiedEndosomal subunit of membrane-associated retromer complexrequired for retrograde transport; receptorthat recognizes retrieval signals on cargoproteins, forms subcomplex with Vps26p andVps29p that selects cargo proteins forretrieval 0.000570.00393&radic&radic S000005519YOL159CUncharacterizedHypothetical protein 0.000570.00393&radic S000005307YNR024WUncharacterizedHypothetical protein 0.000570.00393&radic&radic S000000636MATALPHA1YCR040WverifiedTranscriptional co-activator involved in regulation ofmating-type-specific gene expression; targetsthe transcription factor Mcm1p to the promotersof alpha-specific genes; one of two genesencoded by the MATalpha mating type cassette 0.000570.00393&radic S000007246TMA7YLR262C-AverifiedProtein of unknown that associates with ribosomes; null mutantexhibits translation defects, alteredpolyribosome profiles, and resistance to thetranslation inhibitor anisomcyin 0.000570.00393&radic S000001901SAD1YFR005CverifiedConserved zinc-finger domain protein involved in pre-mRNAsplicing, required for assembly of U4 snRNAinto the U4/U6 particle 0.000570.00393&radic&radic&radic S000002222UBC9YDL064WverifiedSUMO-conjugating enzyme involved in the Smt3p conjugationpathway; nuclear protein required for S- andM-phase cyclin degradation and mitotic control;involved in proteolysis mediated by theanaphase-promoting complex cyclosome (APCC) 0.000570.00393&radic&radic S000004632RSF1YMR030WverifiedProtein required for respiratory growth; localized to both thenucleus and mitochondrion; mutant displaysdecreased transcription of specific nuclear andmitochondrial genes whose products are involvedin respiratory growth 0.000570.00393&radic&radic&radic&radic S000006405ARR3YPR201WverifiedArsenite transporter of the plasma membrane, required forresistance to arsenic compounds; transcriptionis activated by Arr1p in the presence ofarsenite 0.000570.00393&radic S000006362YPR158WUncharacterizedHypothetical protein 0.000570.00392&radic&radic&radic S000005564HIR2YOR038CverifiedNon-essential transcriptional corepressor involved in the cellcycle-regulated transcription of histone H2A,H2B, H3, and H4 genes; recruits Swi-Snfcomplexes to histone gene promoters; promotesheterochromatic gene silencing with Asf1p 0.000570.00392&radic&radic S000000004SSA1YAL005CverifiedATPase involved in protein folding and nuclear localizationsignal (NLS)-directed nuclear transport; memberof heat shock protein 70 (HSP70) family; formsa chaperone complex with Ydj1p; localized tothe nucleus, cytoplasm, and cell wall 0.000570.00392&radic S000004369FBP1YLR377CverifiedFructose-1,6-bisphosphatase, key regulatory enzyme in thegluconeogenesis pathway, required for glucosemetabolism 0.000570.00392&radic&radic S000000074YAR023CUncharacterizedPutative integral membrane protein, member of DUP240 gene family 0.000570.00392&radic S000005377ESC8YOL017WverifiedProtein involved in telomeric and mating-type locus silencing,interacts with Sir2p and also interacts withthe Gal11p, which is a component of the RNA polII mediator complex 0.000570.00392&radic&radic&radic S000002625RAD9YDR217CverifiedDNA damage-dependent checkpoint protein, required forcell-cycle arrest in G1/S, intra-S, and