Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0008170 "N-methyltransferase activity"

GO branch: MF
Number of currently annotated genes: 23

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest Huh:nucleusHarbison:MBF1_YPDLum:GliotoxinLum:DoxycyclineIPR003616IPR000241TAP:108PS50082Lum:CisplatinLum:Dyclonine S000000064SWD1YAR003WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres; WD40beta propeller superfamily member withsimilarity to mammalian Rbbp7 &radic0.412160.96766&radic&radic&radic&radic&radic&radic S000001161SET1YHR119WverifiedHistone methyltransferase, subunit of the COMPASS (Set1C)complex which methylates histone H3 on lysine4; required in transcriptional silencing neartelomeres and at the silent mating type loci;contains a SET domain &radic0.437540.96766&radic&radic&radic S000000379SWD3YBR175WverifiedEssential subunit of the COMPASS (Set1C) complex, whichmethylates histone H3 on lysine 4 and isrequired in transcriptional silencing neartelomeres; WD40 beta propeller superfamilymember and ortholog of mammalian WDR5 &radic0.380580.96766&radic&radic&radic&radic&radic&radic S000002877SDC1YDR469WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres; hassimilarity to C. elegans Dpy-30 &radic0.357220.96659&radic&radic&radic S000004005BRE2YLR015WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres;involved in telomere maintenance; similar totrithorax-group protein ASH2L &radic0.338890.96539&radic&radic&radic&radic&radic&radic S000006059SPP1YPL138CverifiedSubunit of COMPASS (Set1C), a complex which methylates histoneH3 on lysine 4 and is required in telomerictranscriptional silencing; PHD finger domainprotein similar to human CGBP, an unmethylatedCpG binding protein &radic0.244620.91981&radic&radic&radic&radic&radic&radic S000002476DOA4YDR069CverifiedUbiquitin hydrolase, required for recycling ubiquitin fromproteasome-bound ubiquitinated intermediates,acts at the late endosome/prevacuolarcompartment to recover ubiquitin fromubiquitinated membrane proteins en route to thevacuole 0.15320.85078&radic S000001106SSZ1YHR064CverifiedHsp70 protein that interacts with Zuo1p (a DnaJ homolog) toform a ribosome-associated complex that bindsthe ribosome via the Zuo1p subunit; alsoinvolved in pleiotropic drug resistance viasequential activation of PDR1 and PDR5; bindsATP 0.141260.83539&radic&radic S000003874RSM7YJR113CverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S7 ribosomal protein 0.13810.83277&radic S000005333LYS9YNR050CverifiedSaccharopine dehydrogenase (NADP+, L-glutamate-forming);catalyzes the formation of saccharopine fromalpha-aminoadipate 6-semialdehyde, which is theseventh step in lysine biosynthesis pathway 0.06860.70774 S000003704SET2YJL168CverifiedHistone methyltransferase with a role in transcriptionalelongation, methylates a lysine residue ofhistone H3; associates with the C-terminaldomain of Rpo21p; histone methylation activityis regulated by phosphorylation status ofRpo21p &radic0.067370.70397&radic&radic&radic&radic S000003322UTP22YGR090WuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.045260.61226&radic&radic&radic S000005484TRM11YOL124CverifiedCatalytic subunit of an adoMet-dependent tRNA methyltransferasecomplex (Trm11p-Trm112p), required for themethylation of the guanosine nucleotide atposition 10 (m2G10) in tRNAs; contains a THUMPdomain and a methyltransferase domain &radic0.037960.57777&radic&radic S000004476TRM9YML014WverifiedtRNA methyltransferase, catalyzes the esterification ofmodified uridine nucleotides in tRNAs, creating5-methylcarbonylmethyluridine in tRNA(Arg3) and5-methylcarbonylmethyl-2-thiouridine intRNA(Glu); may have a role in stress response 0.029020.51635&radic S000001308MET30YIL046WverifiedF-box protein containing five copies of the WD40 motif,controls cell cycle function, sulfurmetabolism, and methionine biosynthesis as partof the ubiquitin ligase complex; interacts withand regulates Met4p, localizes within thenucleus 0.017380.41373&radic&radic&radic&radic&radic S000004421CRN1YLR429WverifiedCoronin, cortical actin cytoskeletal component that associateswith the Arp2p/Arp3p complex to regulate itsactivity 0.016240.40232&radic&radic S000003196VID30YGL227WverifiedProtein involved in proteasome-dependent catabolite degradationof fructose-1,6-bisphosphatase (FBPase); shiftsthe balance of nitrogen metabolism toward theproduction of glutamate; localized to thenucleus and the cytoplasm 0.015850.39804&radic&radic S000005329TRM112YNR046WverifiedSubunit of an adoMet-dependent tRNA methyltransferase (MTase)complex (Trm11p-Trm112p), required for themethylation of the guanosine nucleotide atposition 10 (m2G10) in tRNAs; putative zincbinding subunit of other MTase-related proteins &radic0.015740.39804&radic&radic S000002547MTQ2YDR140WuncharacterizedS-adenosylmethionine-dependent methyltransferase of the sevenbeta-strand family; methylates release factoreRF1 (Sup45p) in vitro; is not an essentialgene; similar to E.coli PrmC 0.015320.39317&radic&radic&radic&radic S000005261PFS2YNL317WverifiedIntegral subunit of the pre-mRNA cleavage and polyadenylationfactor (CPF) complex; plays an essential rolein mRNA 3'-end formation by bridging differentprocessing factors and thereby promoting theassembly of the processing complex 0.015050.38996&radic&radic&radic&radic S000003432ELP2YGR200CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;target of Kluyveromyces lactis zymocin 0.014340.36741&radic&radic&radic S000001607SSH4YKL124WverifiedProtein whose overexpression confers resistance to the growthinhibitor leflunomide; suppressor of shr3mutation 0.012990.35487&radic S000004781YMR171CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to endosomes; mRNA is targeted to the bud via the mRNA transport system involving She2p: YMR171C is not an essential gene 0.011060.32738 S000002971CDH1YGL003CverifiedCell-cycle regulated activator of the anaphase-promotingcomplex/cyclosome (APC/C), which directsubiquitination of mitotic cyclins resulting inexit from mitosis; targets the APC/C tospecific substrates including CDC20, ASE1 andCIN8 0.010730.32388&radic&radic S000000214HHT1YBR010WverifiedOne of two identical histone H3 proteins (see also HHT2); corehistone required for chromatin assembly,involved in heterochromatin-mediated telomericand HM silencing; regulated by acetylation,methylation, and mitotic phosphorylation 0.009540.30194&radic S000001737SET3YKR029CverifiedDefining member of the SET3 histone deacetylase complex whichis a meiosis-specific repressor of sporulationgenes; necessary for efficient transcription byRNAPII; one of two yeast proteins that containsboth SET and PHD domains 0.008870.29308&radic&radic S000004212UTP13YLR222CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.008790.29066&radic&radic&radic&radic S000002665SET7YDR257CverifiedNuclear protein that contains a SET-domain, which have beenshown to mediate methyltransferase activity inother proteins 0.008770.29066&radic&radic S000003934SOF1YLL011WverifiedEssential subunit of the U3 (box C+D) snRNP complex requiredfor 2' O-methylation of pre-RNA; has similarityto the beta subunit of trimeric G-proteins andthe splicing factor Prp4p 0.008840.29066&radic&radic&radic&radic&radic&radic S000003105SEC27YGL137WverifiedEssential beta'-coat protein of the COPI coatomer, involved inER-to-Golgi and Golgi-to-ER transport; containsWD40 domains that mediate cargo selectiveinteractions; 45% sequence identity tomammalian beta'-COP 0.00870.28695&radic&radic S000004401UTP21YLR409CuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.008170.27913&radic&radic&radic&radic&radic S000004119DIP2YLR129WverifiedNucleolar protein, specifically associated with the U3 snoRNA,part of the large ribonucleoprotein complexknown as the small subunit (SSU) processome,required for 18S rRNA biogenesis, part of theactive pre-rRNA processing complex 0.007910.27596&radic&radic&radic&radic&radic S000000104HIR1YBL008WverifiedNon-essential transcriptional corepressor involved in the cellcycle-regulated transcription of histone H2A,H2B, H3 and H4 genes; contributes to nucleosomeformation, heterochromatic gene silencing, andformation of functional kinetochores 0.007820.27474&radic&radic&radic&radic S000005372HTZ1YOL012CverifiedHistone variant H2AZ, exchanged for histone H2A in nucleosomesby the SWR1 complex; involved intranscriptional regulation through preventionof the spread of silent heterochromatin 0.007830.27474&radic&radic S000006382PRP4YPR178WverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.00760.26882&radic&radic&radic S000000452HIS7YBR248CverifiedImidazole glycerol phosphate synthase (glutamineamidotransferase:cyclase), catalyzes the fifthand sixth steps of histidine biosynthesis andalso produces 5-aminoimidazole-4-carboxamideribotide (AICAR), a purine precursor 0.007430.26589&radic&radic&radic&radic S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 0.007380.26589&radic&radic&radic&radic&radic S000004570CAC2YML102WverifiedComponent of the chromatin assembly complex (with Rlf2p andMsi1p) that assembles newly synthesizedhistones onto recently replicated DNA, requiredfor building functional kinetochores, conservedfrom yeast to humans 0.007490.26589&radic&radic S000002772CDC40YDR364CverifiedPre-mRNA splicing factor, important for catalytic step II ofpre-mRNA splicing and plays a role in cellcycle progression; required for DNA synthesisduring mitosis and meiosis; has WD repeats 0.007120.25674&radic&radic&radic&radic S000003783LSM8YJR022WverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)that is part of spliceosomal U6 snRNP and isalso implicated in processing of pre-tRNA,pre-snoRNA, and pre-rRNA 0.007310.25674&radic&radic S000001696DOA1YKL213CverifiedWD repeat protein required for ubiquitin-mediated proteindegradation, forms complex with Cdc48p, plays arole in controlling cellular ubiquitinconcentration; also promotes efficient NHEJ inpostdiauxic/stationary phase 0.00660.25122&radic&radic&radic&radic S000001129YHR087WVerifiedProtein involved in RNA metabolism, one of two yeast homologs (with Sdo1p/Ylr022cp) of the human protein SBDS responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome, also conserved in Archaea 0.006770.25122&radic&radic&radic&radic S000005738STE4YOR212WverifiedG protein beta subunit, forms a dimer with Ste18p to activatethe mating signaling pathway, forms aheterotrimer with Gpa1p and Ste18p to dampensignaling; may recruit Rho1p to the polarizedgrowth site during mating; contains WD40repeats 0.00680.25122&radic&radic&radic S000000183RPL23AYBL087CverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl23Bp and has similarity to E.coli L14 and rat L23 ribosomal proteins 0.006670.25122&radic&radic S000002972RPN14YGL004CuncharacterizedPutative non-ATPase subunit of the 19S regulatory particle ofthe 26S proteasome; localized to the cytoplasm 0.006760.25122&radic&radic&radic S000004754TIF34YMR146CverifiedSubunit of the core complex of translation initiation factor3(eIF3), which is essential for translation 0.006140.24048&radic&radic S000004951LST8YNL006WverifiedProtein required for the transport of amino acid permease Gap1pfrom the Golgi to the cell surface; componentof the TOR signaling pathway; associates withboth Tor1p and Tor2p; contains a WD-repeat 0.006310.24048&radic&radic S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.006180.24048&radic&radic&radic&radic S000003723SWE1YJL187CverifiedProtein kinase that regulates the G2/M transition by inhibitionof Cdc28p kinase activity; localizes to thenucleus and to the daughter side of themother-bud neck; homolog of S. pombe Wee1p;potential Cdc28p substrate 0.006280.24048&radic S000000307SIF2YBR103WverifiedWD40 repeat-containing subunit of the Set3C histone deacetylasecomplex, which represses early/middlesporulation genes; antagonizes telomericsilencing; binds specifically to the Sir4pN-terminus 0.006060.23902&radic&radic&radic S000001744CAF4YKR036CverifiedWD40 repeat-containing protein associated with the CCR4-NOTcomplex, interacts in a Ccr4p-dependent mannerwith Ssn2p; also interacts with Fis1p, Mdv1pand Dnm1p and plays a role in mitochondrialfission 0.006070.23902&radic&radic S000002304COP1YDL145CverifiedAlpha subunit of COPI vesicle coatomer complex, which surroundstransport vesicles in the early secretorypathway 0.006070.23902&radic&radic S000003181SKI8YGL213CverifiedProtein involved in exosome mediated 3' to 5' mRNA degradationand translation inhibition of non-poly(A) mRNAsas well as double-strand break formation duringmeiotic recombination; required for repressingpropagation of dsRNA viruses 0.006060.23902&radic&radic S000003992NOC3YLR002CverifiedProtein that forms a nuclear complex with Noc2p that binds to66S ribosomal precursors to mediate theirintranuclear transport; also binds to chromatinto promote the association of DNA replicationfactors and replication initiation 0.005980.23708&radic&radic&radic S000005895RAD17YOR368WverifiedCheckpoint protein, involved in the activation of the DNAdamage and meiotic pachytene checkpoints; withMec3p and Ddc1p, forms a clamp that is loadedonto partial duplex DNA; homolog of human andS. pombe Rad1 and U. maydis Rec1 proteins 0.005670.22972&radic&radic&radic S000004722ASC1YMR116CverifiedWD repeat protein (G-beta like protein) involved in translationregulation; required for repression of Gcn4pactivity in the absence of amino-acidstarvation; core component of the ribosome;ortholog of mammalian RACK1 0.005880.22972&radic&radic S000004708YMR102CUncharacterizedProtein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene 0.005690.22972&radic&radic S000002769BCP1YDR361CverifiedEssential protein involved in nuclear export of Mss4p, which isa lipid kinase that generatesphosphatidylinositol 4,5-biphosphate and playsa role in actin cytoskeleton organization andvesicular transport 0.005820.22972&radic&radic&radic S000006341RRP9YPR137WverifiedProtein involved in pre-rRNA processing, associated with U3snRNP; component of small ribosomal subunit(SSU) processosome; ortholog of the humanU3-55k protein 0.00580.22972&radic S000004279MEC3YLR288CverifiedDNA damage and meiotic pachytene checkpoint protein; subunit ofa heterotrimeric complex (Rad17p-Mec3p-Ddc1p)that forms a sliding clamp, loaded onto partialduplex DNA by a clamp loader complex; homologof human and S. pombe Hus1 0.005560.22532&radic&radic&radic S000004045SPT8YLR055CverifiedSubunit of the SAGA transcriptional regulatory complex but notpresent in SAGA-like complex SLIK/SALSA,required for SAGA-mediated inhibition at somepromoters 0.005540.22532&radic&radic&radic&radic&radic&radic S000005795PAC1YOR269WverifiedProtein involved in nuclear migration, part of thedynein/dynactin pathway; targets dynein tomicrotubule tips, which is necessary forsliding of microtubules along bud cortex;synthetic lethal with bni1; homolog of humanLIS1 0.005550.22532&radic&radic&radic S000002763YDR355CDubiousHypothetical protein; open reading frame overlaps 5' end of essential SPC110 gene encoding an inner plaque spindle pole body component 0.005530.22532&radic&radic&radic&radic&radic S000002549PEX7YDR142CverifiedPeroxisomal signal receptor for the N-terminal nonapeptidesignal (PTS2) of peroxisomal matrix proteins;WD repeat protein; defects in human homologcause lethal rhizomelic chondrodysplasiapunctata (RCDP) 0.005540.22532&radic&radic S000000653PWP2YCR057CverifiedConserved 90S pre-ribosomal component essential for properendonucleolytic cleavage of the 35 S rRNAprecursor at A0, A1, and A2 sites; containseight WD-repeats; PWP2 deletion leads todefects in cell cycle and bud morphogenesis 0.00550.22468&radic&radic S000004738RRB1YMR131CverifiedEssential nuclear protein involved in early steps of ribosomebiogenesis; physically interacts with theribosomal protein Rpl3p 0.005480.22373&radic&radic&radic S000006104YPL183CUncharacterizedHypothetical protein 0.005440.22343&radic S000000868YER066WUncharacterizedHypothetical protein 0.005290.21837&radic&radic S000004198SEC13YLR208WverifiedComponent of both the Nup84 nuclear pore sub-complex and of theCOPII complex (Sar1p, Sec13p, Sec16p, Sec23p,Sec24p, Sec31p, Sfb2p, and Sfb3p) which isimportant for the formation of ER to Golgitransport vesicles 0.005260.21837&radic&radic&radic S000004410CDC73YLR418CverifiedConstituent of Paf1 complex with RNA polymerase II, Paf1p,Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinctfrom Srb-containing Pol II complexes; requiredfor expression of certain genes, modificationof some histones, and telomere maintenance 0.005270.21837&radic&radic&radic&radic&radic S000001069RPN1YHR027CverifiedNon-ATPase base subunit of the 19S regulatory particle of the26S proteasome; may participate in therecognition of several ligands of theproteasome; contains a leucine-rich repeat(LRR) domain, a site for protein?proteininteractions 0.005210.21665&radic S000000668RSA4YCR072CverifiedWD-repeat protein involved in ribosome biogenesis; may interactwith ribosomes; required for maturation andefficient intra-nuclear transport or pre-60Sribosomal subunits, localizes to the nucleolus 0.005160.21631&radic S000003068SEH1YGL100WverifiedNuclear pore protein that is part of the evolutionarilyconserved Nup84p complex (Nup84p, Nup85p,Nup120p, Nup145p, and Seh1p); homologous toSec13p 0.005140.21545&radic S000001504MAK11YKL021CverifiedProtein essential for cell growth and replication of M dsRNAvirus; contains four beta-transducin repeats 0.005080.21376&radic&radic&radic S000003648MDV1YJL112WverifiedPeripheral protein of the cytosolic face of the mitochondrialouter membrane, required for mitochondrialfission; interacts with Fis1p and with thedynamin-related GTPase Dnm1p; contains WDrepeats 0.005020.21249&radic S000003084CDC20YGL116WverifiedCell-cycle regulated activator of anaphase-promotingcomplex/cyclosome (APC/C), which is requiredfor metaphase/anaphase transition; directsubiquitination of mitotic cyclins, Pds1p, andother anaphase inhibitors; potential Cdc28psubstrate 0.005030.21249&radic&radic&radic S000001146GRE3YHR104WverifiedAldose reductase involved in methylglyoxal, d-xylose andarabinose metabolism; stress induced (osmotic,ionic, oxidative, heat shock, starvation andheavy metals); regulated by the HOG pathway 0.004930.20962&radic&radic&radic S000002537FIN1YDR130CverifiedSpindle pole body-related intermediate filament protein, formscell cycle-specific filaments between spindlepole bodies in mother and daughter cells, ableto self-assemble, expression induced duringS/G2, localization cell-cycle dependent 0.004950.20962&radic&radic&radic&radic S000000462SHG1YBR258CverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres &radic0.00490.20962&radic&radic&radic&radic&radic S000004629YMR027WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene 0.004860.20748&radic&radic&radic&radic&radic S000005945RMI1YPL024WverifiedInvolved in response to DNA damage; null mutants have increasedrates of recombination and delayed S phase;interacts physically and genetically with Sgs1p(RecQ family member) and Top3p (topoisomeraseIII) 0.004880.20748&radic&radic&radic S000000449ISW1YBR245CverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPase thatforms a complex with Ioc2p and Ioc4p toregulate transcription elongation, and acomplex with Ioc3p to repress transcriptioninitiation 0.004880.20748&radic&radic&radic&radic S000003782REC107YJR021CverifiedProtein involved in early stages of meiotic recombination;involved in altering chromatin structure at DNAdouble-stranded break sites and in coordinationbetween the initiation of recombination and thefirst division of meiosis 0.004770.20509&radic S000001604YKL121WUncharacterizedPutative protein of unknown function 0.004790.20509&radic&radic&radic S000000680TUP1YCR084CverifiedGeneral repressor of transcription, forms complex with Cyc8p,involved in the establishment of repressivechromatin structure through interactions withhistones H3 and H4, appears to enhanceexpression of some genes 0.004770.20509&radic&radic S000005677RPB2YOR151CverifiedRNA polymerase II second largest subunit B150, part of centralcore; similar to bacterial beta subunit 0.004750.20437&radic&radic&radic&radic S000000851TPA1YER049WuncharacterizedProtein of unknown function; interacts with Sup45p (eRF1),Sup35p (eRF3) and Pab1p; has a role intranslation termination efficiency, mRNApoly(A) tail length and mRNA stability 0.004660.20152&radic&radic&radic&radic S000001885CDC4YFL009WverifiedF-box protein required for G1/S and G2/M transition, associateswith Skp1p and Cdc53p to form a complex,SCFCdc4, which acts as ubiquitin-protein ligasedirecting ubiquitination of the phosphorylatedCDK inhibitor Sic1p 0.004730.20152&radic S000000805PMI40YER003CverifiedMannose-6-phosphate isomerase, catalyzes the interconversion offructose-6-P and mannose-6-P; required forearly steps in protein mannosylation 0.00460.20048&radic&radic&radic&radic&radic S000004840TAF7YMR227CverifiedTFIID subunit (67 kDa), involved in RNA polymerase IItranscription initiation 0.004530.19891&radic&radic S000006072PRP46YPL151CverifiedSplicing factor that is found in the Cef1p subcomplex of thespliceosome 0.004550.19891&radic&radic&radic S000000438ARC40YBR234CverifiedEssential subunit of the ARP2/3 complex, which is required forthe motility and integrity of cortical actinpatches 0.004490.19757&radic&radic S000003641SET4YJL105WverifiedProtein of unknown function, contains a SET domain 0.004520.19757&radic S000005798YTM1YOR272WverifiedConstituent of 66S pre-ribosomal particles, required formaturation of the large ribosomal subunit 0.004470.19651&radic&radic S000005649LEO1YOR123CverifiedComponent of the Paf1 complex, which associates with RNApolymerase II and is involved in histonemethylation 0.004430.19617&radic&radic&radic S000001031YHL039WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.004350.19244&radic S000004761YMR153C-ADubiousHypothetical protein 0.004310.19244&radic&radic&radic S000004901HSH155YMR288WverifiedU2-snRNP associated splicing factor that forms extensiveassociations with the branch site-3' splicesite-3' exon region upon prespliceosomeformation; similarity to the mammalian U2snRNP-associated splicing factor SAP155 0.004370.19244&radic&radic&radic S000000456DUT1YBR252WverifieddUTPase, catalyzes hydrolysis of dUTP to dUMP and PPi, therebypreventing incorporation of uracil into DNAduring replication; critical for themaintenance of genetic stability 0.004230.18913&radic&radic&radic S000000173YBL077WDubiousHypothetical protein; open reading frame overlaps 3' end of essential ILS1 gene encoding cytoplasmic isoleucine-tRNA synthetase 0.004190.18913&radic&radic S000003367PRE9YGR135Wverified20S proteasome beta-type subunit; the only nonessential 20Ssubunit 0.004280.18913&radic&radic&radic S000006132NIP7YPL211WverifiedNucleolar protein required for 60S ribosome subunit biogenesis,constituent of 66S pre-ribosomal particles;physically interacts with Nop8p and the exosomesubunit Rrp43p 0.004240.18913&radic&radic S000003388PTI1YGR156WverifiedPta1p Interacting protein 0.003990.18179&radic&radic&radic S000001132YNG2YHR090CverifiedSubunit of the NuA4 histone acetyltransferase complex thatacetylates histone H4 and H2A; has similarityto the human tumor suppressor ING1 0.004050.18179&radic S000002742SWR1YDR334WverifiedSwi2/Snf2-related ATPase that is the structural component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.003950.18179&radic&radic S000000149YBL053WDubiousHypothetical protein 0.004010.18179&radic&radic&radic S000005820RRS1YOR294WverifiedEssential protein that binds ribosomal protein L11 and isrequired for nuclear export of the 60Spre-ribosomal subunit during ribosomebiogenesis; mouse homolog shows alteredexpression in Huntington's disease model mice 0.004140.18179&radic&radic&radic S000002603REF2YDR195WverifiedRNA-binding protein involved in the cleavage step of mRNA3'-end formation prior to polyadenylation, andin snoRNA maturation; part of holo-CPFsubcomplex APT, which associates with 3'-endsof snoRNA- and mRNA-encoding genes 0.004160.18179&radic&radic&radic&radic S000003307PRP38YGR075CverifiedUnique component of the U4/U6.U5 tri-snRNP particle,dispensable for spliceosome assembly, butrequired for conformational changes which leadto catalytic activation of the spliceosome 0.004140.18179&radic&radic&radic&radic&radic S000000782HAT2YEL056WverifiedSubunit of the Hat1p-Hat2p histone acetyltransferase complex;required for high affinity binding of thecomplex to free histone H4, thereby enhancingHat1p activity; similar to human RbAp46 and 48;has a role in telomeric silencing 0.004050.18179&radic&radic&radic&radic S000002632HTB1YDR224CverifiedOne of two nearly identical (see HTB2) histone H2B subtypesrequired for chromatin assembly and chromosomefunction; Rad6p-Bre1p-Lge1p mediatedubiquitination regulates transcriptionalactivation, meiotic DSB formation and H3methylation 0.003950.18179&radic&radic&radic S000001250SET5YHR207CverifiedZinc-finger protein of unknown function, contains one canonicaland two unusual fingers in unusualarrangements; deletion enhances replication ofpositive-strand RNA virus 0.004030.18179&radic&radic S000000402TAF5YBR198CverifiedSubunit (90 kDa) of TFIID and SAGA complexes, involved in RNApolymerase II transcription initiation and inchromatin modification 0.004150.18179&radic&radic&radic S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 0.003950.18179&radic&radic&radic&radic S000002675CIA1YDR267CverifiedEssential protein involved in assembly of cytosolic and nucleariron-sulfur proteins 0.003930.18162&radic S000004652ERB1YMR049CverifiedProtein required for maturation of the 25S and 5.8S ribosomalRNAs; constituent of 66S pre-ribosomalparticles; homologous to mammalian Bop1 0.003910.18042&radic&radic S000005682NFI1YOR156CverifiedSUMO ligase, catalyzes the covalent attachment of SUMO (Smt3p)to proteins; involved in maintenance of propertelomere length 0.003920.18042&radic&radic&radic S000002527TRM1YDR120CverifiedtRNA methyltransferase, localizes to both the nucleus andmitochondrion to produce the modified baseN2,N2-dimethylguanosine in tRNAs in bothcompartments &radic0.003880.18009&radic&radic&radic&radic&radic S000002299RPO21YDL140CverifiedRNA polymerase II largest subunit B220, part of central core;phosphorylation of C-terminal heptapeptiderepeat domain regulates association withtranscription and splicing factors; similar tobacterial beta-prime 0.003850.17911&radic&radic S000006130IPL1YPL209CverifiedAurora kinase involved in regulating kinetochore-microtubuleattachments; helps maintain condensedchromosomes during anaphase and earlytelophase; associates with Sli15p, whichpromotes Ipl1p association with mitotic spindle 0.00380.17781&radic&radic&radic S000006086SET6YPL165CverifiedProtein of unknown function; deletion heterozygote is sensitiveto compounds that target ergosterolbiosynthesis, may be involved in compoundavailability 0.003820.17781 S000003233YGR001CUncharacterizedPutative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae 0.003780.17762 S000002765YDR357CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 0.003750.17601&radic&radic&radic&radic S000003833NPA3YJR072CverifiedEssential, conserved, cytoplasmic ATPase; phosphorylated by thePcl1p-Pho85p kinase complex 0.003710.1751&radic S000000730YEA4YEL004WverifiedUridine diphosphate-N-acetylglucosamine (UDP-GlcNAc)transporter required for cell wall chitinsynthesis; localized to the ER 0.003690.1751&radic&radic&radic S000003026RAD6YGL058WverifiedUbiquitin-conjugating enzyme (E2), involved in postreplicationrepair (with Rad18p), sporulation, telomeresilencing, and ubiquitin-mediated N-end ruleprotein degradation (with Ubr1p) 0.003660.17383&radic&radic&radic&radic S000005205ORC5YNL261WverifiedSubunit of the origin recognition complex, which directs DNAreplication by binding to replication originsand is also involved in transcriptionalsilencing 0.003670.17383&radic&radic&radic&radic&radic&radic S000005392OPI10YOL032WverifiedProtein with a possible role in phospholipid biosynthesis,based on inositol-excreting phenotype of thenull mutant and its suppression by exogenouscholine 0.003660.17383&radic&radic&radic S000005007MTQ1YNL063WuncharacterizedS-adenosylmethionine-dependent Methyltransferase; methylatestranslational release factor Mrf1p; similar toE.coli PrmC; is not an essential gene 0.003650.17383&radic S000000935GLC7YER133WverifiedCatalytic subunit of type 1 serine/threonine proteinphosphatase, involved in many processesincluding glycogen metabolism, sporulation, andmitosis; interacts with multiple regulatorysubunits; predominantly isolated with Sds22p 0.003650.17383&radic&radic S000000886YER084WDubiousHypothetical protein 0.003670.17383&radic&radic&radic&radic&radic S000003038RPB9YGL070CverifiedRNA polymerase II subunit B12.6; contacts DNA; mutations affecttranscription start site; involved in telomeremaintenance 0.003640.17322&radic&radic&radic&radic S000004300IMH1YLR309CverifiedProtein involved in vesicular transport, mediates transportbetween an endosomal compartment and the Golgi,contains a Golgi-localization (GRIP) domainthat interacts with activated Arl1p-GTP tolocalize Imh1p to the Golgi 0.003640.17322&radic&radic S000002777XRS2YDR369CverifiedProtein required for DNA repair; component of the Mre11complex, which is involved in double strandbreaks, meiotic recombination, telomeremaintenance, and checkpoint signaling 0.003630.17168&radic&radic&radic S000003335NOP7YGR103WverifiedNucleolar protein involved in rRNA processing and 60S ribosomalsubunit biogenesis; constituent of severaldifferent pre-ribosomal particles; required forexit from G0 and the initiation ofcell proliferation 0.003520.16815&radic&radic&radic S000004069SIC1YLR079WverifiedInhibitor of Cdc28-Clb kinase complexes that controls G1/Sphase transition, preventing premature S phaseand ensuring genomic integrity; phosphorylationtargets Sic1p for SCF(CDC4)-dependent turnover;functional homolog of mammalian Kip1 0.00350.16815&radic&radic&radic S000002545HPR1YDR138WverifiedSubunit of THO/TREX complexes that couple transcriptionelongation with mitotic recombination and withmRNA metabolism and export, subunit of an RNAPol II complex; regulates lifespan; involved intelomere maintenance; similar to Top1p 0.003570.16815&radic&radic&radic&radic S000002232BRE1YDL074CverifiedE3 ubiquitin ligase for Rad6p, required for the ubiquitinationof histone H2B, recruitment of Rad6p topromoter chromatin and subsequent methylationof histone H3 (on L4 and L79), contains RINGfinger domain 0.003480.16815&radic&radic S000003011DST1YGL043WverifiedGeneral transcription elongation factor TFIIS, enables RNApolymerase II to read through blocks toelongation by stimulating cleavage of nascenttranscripts stalled at transcription arrestsites 0.003480.16815&radic&radic&radic S000004070EMP46YLR080WverifiedIntegral membrane component of endoplasmic reticulum-derivedCOPII-coated vesicles, which function in ER toGolgi transport 0.00340.16453&radic&radic S000003682IDS2YJL146WverifiedProtein involved in modulation of Ime2p activity duringmeiosis, appears to act indirectly to promoteIme2p-mediated late meiotic functions; found ingrowing cells and degraded during sporulation 0.003440.16453&radic&radic&radic&radic S000002543VPS61YDR136CdubiousDubious open reading frame, unlikely to encode a protein; notconserved in closely related Saccharomycesspecies; 4% of ORF overlaps the verified geneRGP1; deletion causes a vacuolar proteinsorting defect 0.003390.16453&radic&radic&radic&radic S000005202DSL1YNL258CverifiedEndoplasmic reticulum (ER)-localized peripheral membraneprotein required for Golgi-to-ER retrogradetraffic; component of the ER target site thatinteracts with coatomer, the major component ofthe COPI vesicle protein coat 0.00340.16453&radic&radic&radic&radic&radic S000000157SKT5YBL061CverifiedActivator of Chs3p (chitin synthase III), recruits Chs3p to thebud neck via interaction with Bni4p; hassimilarity to Shc1p, which activates Chs3pduring sporulation 0.003410.16453&radic&radic&radic&radic S000006311YTH1YPR107CverifiedEssential RNA-binding component of cleavage and polyadenylationfactor, contains five zinc fingers; requiredfor pre-mRNA 3'-end processing andpolyadenylation 0.003380.16453&radic&radic S000002200SIR2YDL042CverifiedConserved NAD+ dependent histone deacetylase of the Sirtuinfamily involved in regulation of lifespan;plays roles in silencing at HML, HMR,telomeres, and the rDNA locus; negativelyregulates initiation of DNA replication 0.003410.16453&radic S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.003370.16453&radic S000003398KRE11YGR166WverifiedProtein involved in biosynthesis of cell wall beta-glucans;subunit of the TRAPP (transport proteinparticle) complex, which is involved in thelate steps of endoplasmic reticulum to Golgitransport 0.003350.16393&radic&radic S000000975RAD24YER173WverifiedCheckpoint protein, involved in the activation of the DNAdamage and meiotic pachytene checkpoints;subunit of a clamp loader that loadsRad17p-Mec3p-Ddc1p onto DNA; homolog of humanand S. pombe Rad17 protein 0.003360.16393&radic&radic S000005147YNL203CDubiousHypothetical protein 0.003330.16257&radic&radic&radic S000000885GET2YER083CverifiedSubunit of the GET complex; required for meiotic nucleardivision and for the retrieval of HDEL proteinsfrom the Golgi to the ER in an ERD2 dependentfashion; may be involved in cell wall function 0.003340.16257&radic&radic&radic&radic&radic S000003796RAD26YJR035WverifiedProtein involved in transcription-coupled repair nucleotideexcision repair of UV-induced DNA lesions;homolog of human CSB protein 0.003310.16257&radic S000000399MSI1YBR195CverifiedSubunit of chromatin assembly factor I (CAF-I), regulates theRAS/cAMP pathway via sequestration of Npr1pkinase; localizes to the nucleus and cytoplasm;homologous to human retinoblastoma bindingproteins RbAp48 and RbAp46 0.003310.16257&radic&radic&radic&radic&radic&radic S000000041PTA1YAL043CverifiedSubunit of holo-CPF, a multiprotein complex and functionalhomolog of mammalian CPSF, required for thecleavage and polyadenylation of mRNA and snoRNA3' ends; involved in pre-tRNA processing; bindsto the phosphorylated CTD of RNAPII 0.003310.16257&radic&radic&radic&radic S000004914DYN3YMR299CverifiedDynein light intermediate chain (LIC); localizes with dynein,null mutant is defective in nuclear migration 0.00330.16257&radic&radic&radic&radic S000001305CBR1YIL043CverifiedMicrosomal cytochrome b reductase, not essential for viability;also detected in mitochondria; mutation inconserved NADH binding domain of the humanortholog results in type I methemoglobinemia 0.003270.16192&radic&radic&radic S000004460YPT7YML001WverifiedGTPase; GTP-binding protein of the rab family; required forhomotypic fusion event in vacuole inheritance,for endosome-endosome fusion, similar tomammalian Rab7 0.003280.16192&radic&radic S000004376IKI3YLR384CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;maintains structural integrity of Elongator;homolog of human IKAP, mutations in which causefamilial dysautonomia (FD) 0.003270.16192&radic&radic&radic S000002810DIT2YDR402CverifiedN-formyltyrosine oxidase, sporulation-specific microsomalenzyme required for spore wall maturation,involved in the production of a solubleLL-dityrosine-containing precursor of the sporewall, homologous to cytochrome P-450s 0.003280.16192&radic&radic&radic S000005946YPL025CDubiousHypothetical protein 0.003250.16024&radic S000004625MSS1YMR023CverifiedMitochondrial protein, forms a heterodimer complex with Mto1pthat performs the 5-carboxymethylaminomethylmodification of the wobble uridine base inmitochondrial tRNAs; similar to human GTPBP3 0.003220.16024&radic&radic&radic S000001055ARD1YHR013CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.003260.16024&radic&radic S000002982PUF4YGL014WverifiedMember of the PUF protein family, which is defined by thepresence of Pumilio homology domains thatconfer RNA binding activity; preferentiallybinds mRNAs encoding nucleolar ribosomalRNA-processing factors 0.003210.15878&radic S000004240SSP120YLR250WverifiedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmin a punctate pattern 0.00320.15878&radic&radic&radic S000005609WHI5YOR083WverifiedRepressor of G1 transcription that binds to SCB binding factor(SBF) at SCB target promoters in early G1;phosphorylation of Whi5p by the CDK,Cln3p/Cdc28p relieves repression and promoterbinding by Whi5; periodically expressed in G1 0.00320.15878&radic&radic&radic S000001283RPB3YIL021WverifiedRNA polymerase II third largest subunit B44, part of centralcore; similar to prokaryotic alpha subunit 0.003160.15808&radic&radic S000006155TFP3YPL234CverifiedVacuolar ATPase V0 domain subunit c', involved in protontransport activity; hydrophobic integralmembrane protein (proteolipid) containing fourtransmembrane segments; N and C termini are inthe vacuolar lumen 0.003160.15808&radic&radic&radic&radic&radic S000002796RVS167YDR388WverifiedActin-associated protein, subunit of a complex(Rvs161p-Rvs167p) involved in regulation ofactin cytoskeleton, endocytosis, and viabilityfollowing starvation or osmotic stress; homologof mammalian amphiphysin 0.003170.15808&radic&radic S000002625RAD9YDR217CverifiedDNA damage-dependent checkpoint protein, required forcell-cycle arrest in G1/S, intra-S, and G2/M;transmits checkpoint signal by activatingRad53p and Chk1p; hyperphosphorylated by Mec1pand Tel1p; potential Cdc28p substrate 0.003120.1561&radic&radic&radic&radic S000004017AAT2YLR027CverifiedCytosolic aspartate aminotransferase, involved in nitrogenmetabolism; localizes to peroxisomes inoleate-grown cells 0.00310.1561&radic&radic&radic S000004864HOR7YMR251W-AverifiedProtein of unknown function; overexpression suppresses Ca2+sensitivity of mutants lacking inositolphosphorylceramide mannosyltransferases Csg1pand Csh1p; transcription is induced underhyperosmotic stress and repressed by alphafactor 0.003130.1561&radic&radic S000001220YHR177WUncharacterized 0.003150.1561&radic&radic S000004272YLR282CDubiousHypothetical protein 0.003150.1561&radic&radic&radic&radic S000001071YHI9YHR029CverifiedProtein of unknown function that is a member of the PhzFsuperfamily, although unlike its bacterialhomolog, is most likely not involved inphenazine production; possibly involved in amembrane regulation metabolic pathway 0.003130.1561&radic S000006007ELP3YPL086CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;exhibits histone acetyltransferase activitythat is directed to histones H3 and H4;disruption confers resistance to K. lactiszymotoxin 0.003110.1561&radic S000005188SUI1YNL244CverifiedTranslation initiation factor eIF1; component of a complexinvolved in recognition of the initiator codon;modulates translation accuracy at theinitiation phase 0.00310.1561&radic S000004636ARP9YMR033WverifiedActin-related protein involved in transcriptional regulation;subunit of the chromatin remodeling Snf/Swicomplex 0.003060.15427&radic S000000458TRS20YBR254CverifiedOne of 10 subunits of the transport protein particle (TRAPP)complex of the cis-Golgi which mediates vesicledocking and fusion; mutations in the humanhomolog cause the spondyloepiphyseal dysplasiatarda (SEDL) disorder 0.003050.15427&radic&radic&radic S000003716ATP12YJL180CverifiedConserved protein required for assembly of alpha and betasubunits into the F1 sector of mitochondrialF1F0 ATP synthase; mutation of human ATP12reduces active ATP synthase levels and isassociated with the disorder ATPAF2 deficiency 0.003080.15427&radic&radic&radic S000005585YOR059CUncharacterizedHypothetical protein 0.003080.15427&radic&radic S000001566YKL083WDubiousDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 0.003070.15427&radic&radic&radic S000006021ATG21YPL100WverifiedPhosphatidylinositol 3,5-bisphosphate-binding protein requiredfor maturation of pro-aminopeptidase I,predicted to fold as a seven-bladedbeta-propeller; displays punctate cytoplasmiclocalization 0.003090.15427&radic&radic S000001102VMA22YHR060WverifiedIntegral membrane protein that is required for vacuolarH+-ATPase (V-ATPase) function, although not anactual component of the V-ATPase complex;functions in the assembly of the V-ATPase;localized to the yeast endoplasmic reticulum(ER) 0.003050.15427&radic&radic&radic&radic S000002482PPH3YDR075WverifiedCatalytic subunit of an evolutionarily conserved proteinphosphatase complex containing Psy2p and theregulatory subunit Psy4p; required forcisplatin resistance; involved in activation ofGln3p 0.003080.15427&radic&radic&radic&radic S000004871YMR258CUncharacterizedProtein of unknown function with similarity to F-box proteins; physically interacts with Skp1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR258C is not an essential gene 0.003080.15427&radic&radic&radic S000000483PAF1YBR279WverifiedRNA polymerase II-associated protein, defines a large complexthat is biochemically and functionally distinctfrom the Srb-Mediator form of Pol II holoenzymeand is required for full expression of a subsetof cell cycle-regulated genes 0.003060.15427&radic&radic S000003162HOS2YGL194CverifiedHistone deacetylase required for gene activation via specificdeacetylation of lysines in H3 and H4 histonetails; subunit of the Set3 complex, ameiotic-specific repressor of sporulationspecific genes that contains deacetylaseactivity 0.003010.15257&radic&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p 0.003020.15257&radic&radic&radic S000005187SLA2YNL243WverifiedTransmembrane actin-binding protein involved in membranecytoskeleton assembly and cell polarization;adaptor protein that links actin to clathrinand endocytosis; present in the actin corticalpatch of the emerging bud tip; dimer in vivo 0.003050.15257&radic S000003347YGR115CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C 0.003040.15257&radic&radic&radic S000000435SWC5YBR231CuncharacterizedProtein of unknown function, component of the SWR1 complex,which exchanges histone variant H2AZ (Htz1p)for chromatin-bound histone H2A 0.003020.15257&radic&radic&radic S000005197TEX1YNL253WverifiedProtein involved in mRNA export, component of the transcriptionexport (TREX) complex 0.0030.15223&radic&radic&radic&radic&radic&radic S000006324CLB5YPR120CverifiedB-type cyclin involved in DNA replication during S phase;activates Cdc28p to promote initiation of DNAsynthesis; functions in formation of mitoticspindles along with Clb3p and Clb4p; mostabundant during late G1 phase 0.0030.15223&radic&radic S000005378TLG2YOL018CverifiedSyntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1pand mediates fusion of endosome-derivedvesicles with the late Golgi; binds Vps45p,which prevents Tlg2p degradation and alsofacilitates t-SNARE complex formation 0.002990.15207&radic&radic S000003346YGR114CDubiousHypothetical protein; open reading frame overlaps 5' end of essential SPT6 gene involved in nucleosome disassembly and reassembly during transcription 0.002980.1517&radic&radic&radic S000002461CDC34YDR054CverifiedUbiquitin-conjugating enzyme or E2; together with Skp1p, Rbx1p,Cdc53p, and an F-box protein, forms aubiquitin-protein ligase called the SCF complexwhich regulates cell cycle progression bytargeting key substrates for degradation 0.002940.14863&radic&radic&radic S000003453TOS2YGR221CverifiedProtein involved in localization of Cdc24p to the site of budgrowth; may act as a membrane anchor; localizesto the bud neck and bud tip; potentiallyphosphorylated by Cdc28p 0.00290.14863&radic&radic&radic S000003951TPO1YLL028WverifiedPolyamine transporter that recognizes spermine, putrescine, andspermidine; catalyzes uptake of polyamines atalkaline pH and excretion at acidic pH;phosphorylation enhances activity and sortingto the plasma membrane 0.002920.14863&radic&radic&radic S000005210YNL266WDubiousHypothetical protein 0.002940.14863&radic&radic&radic S000000382YBR178WDubiousHypothetical protein 0.002950.14863&radic&radic S000006047NAN1YPL126WverifiedU3 snoRNP protein, component of the small (ribosomal) subunit(SSU) processosome containing U3 snoRNA;required for the biogenesis of18S rRNA 0.002940.14863&radic&radic&radic S000002910SAM2YDR502CverifiedS-adenosylmethionine synthetase, catalyzes transfer of theadenosyl group of ATP to the sulfur atom ofmethionine; one of two differentially regulatedisozymes (Sam1p and Sam2p) 0.002970.14863&radic S000000421ATG12YBR217WverifiedUbiquitin-like modifier, conjugated via an isopeptide bond to alysine residue of Atg5p by the E1 enzyme,Atg7p, and the E2 enzyme, Atg10p, a step thatis essential for autophagy 0.002930.14863&radic&radic S000000358RPB5YBR154CverifiedRNA polymerase subunit ABC27, common to RNA polymerases I, II,and III; contacts DNA and affectstransactivation 0.002930.14863 S000005412SPE2YOL052CverifiedS-adenosylmethionine decarboxylase, required for thebiosynthesis of spermidine and spermine; cellslacking Spe2p require spermine or spermidinefor growth in the presence of oxygen but notwhen grown anaerobically 0.00290.14863&radic&radic S000002522YDR115WUncharacterizedPutative mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins 0.002860.14682&radic&radic&radic&radic S000000999STE20YHL007CverifiedSignal transducing kinase of the PAK (p21-activated kinase)family, involved in pheromone response andpseudohyphal/invasive growth pathways,activated by Cdc42p; binds Ste4p at a GBB motifpresent in noncatalytic domains of PAK kinases 0.002870.14682&radic&radic&radic&radic S000003348SPT6YGR116WverifiedEssential protein that interacts with histones and is involvedin nucleosome disassembly and reassembly duringtranscription elongation 0.002850.14682&radic&radic S000001521RGT1YKL038WverifiedGlucose-responsive transcription factor that regulatesexpression of several glucose transporter (HXT)genes in response to glucose; binds topromoters and acts both as a transcriptionalactivator and repressor 0.002890.14682&radic&radic S000002525APC4YDR118WverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition 0.002850.14682&radic&radic&radic&radic&radic S000005411GAL11YOL051WverifiedComponent of the Mediator complex; interacts with RNApolymerase II and the general transcriptionfactors to form the RNA polymerase IIholoenzyme; affects transcription by acting astarget of activators and repressors 0.002880.14682&radic&radic&radic S000002287YDL129WUncharacterizedHypothetical protein 0.002840.14469&radic S000002789YRA1YDR381WverifiedNuclear protein that binds to RNA and to Mex67p, required forexport of poly(A)+ mRNA from the nucleus;member of the REF (RNA and export factorbinding proteins) family; another familymember, Yra2p, can substitute for Yra1pfunction 0.00280.14469&radic&radic&radic&radic S000001229KOG1YHR186CverifiedSubunit of TORC1, a rapamycin-sensitive complex involved ingrowth control that contains Tor1p or Tor2p,Lst8p and Tco89p; contains four HEAT repeatsand seven WD-40 repeats; may act as a scaffoldprotein to couple TOR and its effectors 0.002790.14469&radic&radic&radic S000001172YHR130CDubiousHypothetical protein 0.00280.14469&radic&radic&radic S000006386SMX3YPR182WverifiedCore Sm protein Sm F; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm F 0.002840.14469&radic&radic&radic S000004798HSC82YMR186WverifiedCytoplasmic chaperone of the Hsp90 family, redundant infunction and nearly identical with Hsp82p, andtogether they are essential; expressedconstitutively at 10-fold higher basal levelsthat HSP82 and induced 2-3 fold by heat shock 0.00280.14469&radic S000001357PRK1YIL095WverifiedProtein serine/threonine kinase; regulates the organization andfunction of the actin cytoskeleton through thephosphorylation of the Pan1p-Sla1p-End3pprotein complex 0.002820.14469&radic&radic S000002893VPS72YDR485CverifiedHtz1p-binding component of the SWR1 complex, which exchangeshistone variant H2AZ (Htz1p) forchromatin-bound histone H2A; required forvacuolar protein sorting 0.00280.14469&radic S000002595YDR187CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W 0.002820.14469&radic&radic&radic S000001781YKR073CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data 0.002780.14209 S000002505GRX3YDR098CverifiedHydroperoxide and superoxide-radical responsiveglutathione-dependent oxidoreductase; monothiolglutaredoxin subfamily member along with Grx4pand Grx5p; protects cells from oxidative damage 0.002740.14209&radic&radic&radic S000000884UTP7YER082CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.002770.14209&radic&radic&radic&radic&radic S000005732NOC2YOR206WverifiedProtein that forms a nucleolar complex with Mak21p that bindsto 90S and 66S pre-ribosomes, as well as anuclear complex with Noc3p that binds to 66Spre-ribosomes; both complexes mediateintranuclear transport of ribosomal precursors 0.002760.14209&radic&radic S000005458YOL098CUncharacterizedPutative metalloprotease 0.002790.14209 S000004630TAP42YMR028WverifiedEssential protein involved in the TOR signaling pathway;physically associates with the proteinphosphatase 2A and the SIT4 protein phosphatasecatalytic subunits 0.002780.14209&radic&radic&radic S000003924DNM1YLL001WverifiedDynamin-related GTPase required for mitochondrial fission andthe maintenance of mitochondrial morphology,assembles on the cytoplasmic face ofmitochondrial tubules at sites at whichdivision will occur; also participates inendocytosis 0.002790.14209&radic&radic&radic&radic S000005950SUV3YPL029WverifiedATP-dependent RNA helicase, component of the mitochondrialdegradosome along with the RNase Dss1p; thedegradosome associates with the ribosome andmediates turnover of aberrant or unprocessedRNAs 0.002750.14209&radic S000000441PRP5YBR237WverifiedRNA helicase in the DEAD-box family, necessary forprespliceosome formation, bridges U1 and U2snRNPs and enables stable U2 snRNP associationwith intron RNA 0.002740.14209&radic&radic&radic&radic S000000213HHF1YBR009CverifiedOne of two identical histone H4 proteins (see also HHF2); corehistone required for chromatin assembly andchromosome function; contributes to telomericsilencing; N-terminal domain involved inmaintaining genomic integrity 0.002740.14209&radic S000005505CTR9YOL145CverifiedComponent of the Paf1p complex, which is a large complex thatbinds to and modulates the activity of RNApolymerase II and is required for expression ofa subset of genes, including cyclin genes;contains TPR repeats 0.002740.14209&radic S000004159YLR169WDubiousHypothetical protein 0.002780.14209&radic&radic&radic&radic S000000009SWC3YAL011WverifiedProtein of unknown function, component of the SWR1 complex,which exchanges histone variant H2AZ (Htz1p)for chromatin-bound histone H2A; required forformation of nuclear-associated array of smoothendoplasmic reticulum known as karmellae 0.002790.14209&radic&radic&radic S000000220YBR016WUncharacterizedPlasma membrane protein of unknown function; has similarity to hydrophilins, which are hydrophilic, glycine-rich proteins involved in the adaptive response to hyperosmotic conditions 0.002740.14209&radic S000002959YER066C-ADubiousDubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W 0.002730.14124&radic&radic&radic&radic S000003853FIP1YJR093CverifiedSubunit of cleavage polyadenylation factor (CPF), interactsdirectly with poly(A) polymerase (Pap1p) toregulate its activity 0.002720.14124&radic S000004161YLR171WDubiousHypothetical protein 0.002730.14124&radic&radic&radic S000002629GTB1YDR221WverifiedGlucosidase II beta subunit, forms a complex with alpha subunitRot2p, involved in removal of two glucoseresidues from N-linked glycans duringglycoprotein biogenesis in the ER 0.00270.14029&radic&radic&radic S000002311YDL152WDubiousHypothetical protein; open reading frame overlaps 5' end of essential SAS10 gene encoding a component of the small ribosomal subunit processosome 0.002710.14029&radic&radic S000004758IMP1YMR150CverifiedCatalytic subunit of the mitochondrial inner membrane peptidasecomplex, required for maturation ofmitochondrial proteins of the intermembranespace; complex contains Imp1p and Imp2p (bothcatalytic subunits), and Som1p 0.00270.14029&radic&radic&radic S000004232ARV1YLR242CverifiedProtein required for normal intracellular sterol distributionand for sphingolipid metabolism; similar toNup120p and C. elegans R05H5.5 protein 0.002710.14029&radic&radic S000005439YOL079WDubiousHypothetical protein 0.00270.14029&radic&radic S000004559GIM5YML094WverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 0.002650.13822 S000001005OTU2YHL013CverifiedProtein of unknown function that may interact with ribosomes,based on co-purification experiments; member ofthe ovarian tumor-like (OTU) superfamily ofpredicted cysteine proteases; shows cytoplasmiclocalization 0.002670.13822&radic&radic&radic&radic S000003295SPT4YGR063CverifiedProtein that forms a complex with Spt5p and mediates bothactivation and inhibition of transcriptionelongation, and plays a role in pre-mRNAprocessing; in addition, Spt4p is involved inkinetochore function and gene silencing 0.002660.13822&radic&radic S000001587GFA1YKL104CverifiedGlutamine-fructose-6-phosphate amidotransferase, catalyzes theformation of glucosamine-6-P and glutamate fromfructose-6-P and glutamine in the first step ofchitin biosynthesis 0.002670.13822&radic&radic S000004436YLR444CDubiousHypothetical protein 0.002670.13822&radic S000001185RPC10YHR143W-AverifiedRNA polymerase subunit, found in RNA polymerase complexes I,II, and III 0.002670.13822&radic&radic S000000229YBR025CUncharacterizedHypothetical protein 0.002690.13822&radic S000000316CYC8YBR112CverifiedGeneral transcriptional co-repressor, acts together with Tup1p;also acts as part of a transcriptionalco-activator complex that recruits the SWI/SNFand SAGA complexes to promoters 0.002660.13822&radic&radic S000005605ATX2YOR079CverifiedGolgi membrane protein involved in manganese homeostasis;overproduction suppresses the sod1 (copper,zinc superoxide dismutase) null mutation 0.002680.13822&radic&radic&radic S000006161HSP82YPL240CverifiedCytoplasmic chaperone (Hsp90 family) required for pheromonesignaling and negative regulation of Hsf1p;docks with the mitochondrial import receptorTom70p for preprotein delivery; interacts withco-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p 0.002640.13634&radic S000002848DOT1YDR440WverifiedNucleosomal histone H3-Lys79 methylase, associates withtranscriptionally active genes, functions ingene silencing at telomeres, most likely bydirectly modulating chromatin structure and Sirprotein localization &radic0.00260.13634&radic S000002597SLY1YDR189WverifiedHydrophilic protein involved in vesicle trafficking between theER and Golgi; SM (Sec1/Munc-18) family proteinthat binds the tSNARE Sed5p and stimulates itsassembly into a trans-SNARE membrane-proteincomplex 0.002630.13634&radic&radic&radic S000003213RTF1YGL244WverifiedSubunit of the RNA polymerase II-associated Paf1 complex;directly or indirectly regulates DNA-bindingproperties of Spt15p and relative activities ofdifferent TATA elements; involved in telomeremaintenance 0.002610.13634&radic&radic S000001458MUC1YIR019CverifiedGPI-anchored cell surface glycoprotein required for diploidpseudohyphal formation and haploid invasivegrowth, transcriptionally regulated by the MAPKpathway (via Ste12p and Tec1p) and the cAMPpathway (via Flo8p) 0.002610.13634&radic S000005686MTR10YOR160WverifiedNuclear import receptor, mediates the nuclear localization ofproteins involved in mRNA-nucleus export 0.002550.13362&radic&radic&radic S000000036CDC19YAL038WverifiedPyruvate kinase, functions as a homotetramer in glycolysis toconvert phosphoenolpyruvate to pyruvate, theinput for aerobic (TCA cycle) or anaerobic(glucose fermentation) respiration 0.002560.13362&radic&radic&radic&radic S000005126IPI3YNL182CuncharacterizedProtein required for cell viability; computational analysis oflarge-scale protein-protein interaction datasuggests a possible role in assembly of theribosomal large subunit 0.002540.13362&radic&radic S000000754YEL028WDubiousHypothetical protein 0.002550.13362&radic&radic&radic S000004258SEC22YLR268WverifiedR-SNARE protein; assembles into SNARE complex with Bet1p, Bos1pand Sed5p; cycles between the ER and Golgicomplex; involved in anterograde and retrogradetransport between the ER and Golgi;synaptobrevin homolog 0.002550.13362&radic&radic S000000122LSM2YBL026WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.002570.13362&radic&radic S000002198NAT1YDL040CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.002550.13362 S000004274ECI1YLR284CverifiedPeroxisomal delta3,delta2-enoyl-CoA isomerase, hexamericprotein that converts 3-hexenoyl-CoA totrans-2-hexenoyl-CoA, essential for thebeta-oxidation of unsaturated fatty acids,oleate-induced 0.002560.13362&radic&radic&radic&radic S000003413TIM13YGR181WverifiedMitochondrial intermembrane space protein, forms a complex withTIm8p that mediates import and insertion of asubset of polytopic inner membrane proteins;may prevent aggregation of incoming proteins ina chaperone-like manner 0.002580.13362&radic&radic S000000905SSA4YER103WverifiedHeat shock protein that is highly induced upon stress; plays arole in SRP-dependent cotranslationalprotein-membrane targeting and translocation;member of the HSP70 family; cytoplasmic proteinthat concentrates in nuclei upon starvation 0.002560.13362 S000000806FMP52YER004WverifiedProtein of unknown function, localized to the mitochondrialouter membrane 0.002580.13362&radic S000006159YPL238CDubiousHypothetical protein; open reading frame overlaps 5' end of essential SUI3 gene encoding a translation initiation factor subunit 0.002580.13362&radic S000005386MIM1YOL026CverifiedMitochondrial outer membrane protein, required for assembly ofthe translocase of the outer membrane (TOM)complex and thereby for mitochondrial proteinimport; N terminus is exposed to the cytosol:transmembrane segment is highly conserved 0.002540.13345&radic S000000076YAR028WUncharacterizedPutative integral membrane protein, member of DUP240 gene family 0.002540.13345&radic&radic&radic&radic S000005750RPB8YOR224CverifiedRNA polymerase subunit ABC14.5, common to RNA polymerases I,II, and III 0.002520.13209&radic S000002740IRC3YDR332WuncharacterizedHypothetical protein; null mutant displays increased levels ofspontaneous Rad52 foci 0.002530.13209&radic S000002491TVP23YDR084CuncharacterizedIntegral membrane protein localized to late Golgi vesiclesalong with the v-SNARE Tlg2p; green fluorescentprotein (GFP)-fusion protein localizes to thecytoplasm in a punctate pattern 0.002520.13209&radic&radic&radic S000006154NSL1YPL233WverifiedEssential component of the MIND kinetochore complex (Mtw1pIncluding Nnf1p-Nsl1p-Dsn1p) which joinskinetochore subunits contacting DNA to thosecontacting microtubules; required for accuratechromosome segregation 0.002530.13209&radic&radic&radic&radic S000001111RRP4YHR069CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp43p and Dis3p 0.002520.13209&radic&radic&radic S000000736YEL010WDubiousHypothetical protein 0.002530.13209&radic&radic&radic S000004976HHT2YNL031CverifiedOne of two identical histone H3 proteins (see also HHT1); corehistone required for chromatin assembly,involved in heterochromatin-mediated telomericand HM silencing; regulated by acetylation,methylation, and mitotic phosphorylation 0.002520.13209&radic S000002464YOS9YDR057WverifiedLectin; soluble lumenal ER protein; member of the OS-9 proteinfamily; similar to mannose-6-phosphatereceptors (MPRs); serves as a receptor thatrecognizes misfolded N-glycosylated proteinsand participates in their targeting to ERAD 0.002520.13209&radic&radic&radic S000000388YBR184WUncharacterizedPutative protein of unknown function; YBR184W is not an essential gene 0.002520.13209&radic&radic&radic S000002633HTA1YDR225WverifiedOne of two nearly identical (see also HTA2) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.002530.13209&radic S000001083SRB2YHR041CverifiedGeneral transcription factor, subunit of the RNA polymerase IImediator complex; associates with corepolymerase subunits to form the RNA polymeraseII holoenzyme; involved in telomere maintenance 0.002490.13108&radic S000005691SEY1YOR165WverifiedProtein of unknown function, contains two predicted GTP-bindingmotifs GXXXXGKS and DXXG near the N-terminus,homolog of the Arabidopsis gene RHD3 (Root HairDefective) 0.002510.13108&radic&radic S000004314VPS65YLR322WdubiousDubious open reading frame, unlikely to encode a protein; notconserved in closely related Saccharomycesspecies; 75% of ORF overlaps the verified geneSFH1; deletion causes a vacuolar proteinsorting defect 0.002490.13108&radic&radic S000002800SPT3YDR392WverifiedSubunit of the SAGA and SAGA-like transcriptional regulatorycomplexes, interacts with Spt15p to activatetranscription of some RNA polymeraseII-dependent genes, also functions to inhibittranscription at some promoters 0.00250.13108&radic&radic&radic S000003072VPS73YGL104CverifiedMitochondrial protein of unknown function involved in vacuolarprotein sorting 0.00250.13108&radic S000002611YDR203WDubiousHypothetical protein 0.00250.13108&radic&radic S000004829SKY1YMR216CverifiedSR protein kinase (SRPK) involved in regulating proteinsinvolved in mRNA metabolism and cationhomeostasis; similar to human SRPK1 0.002510.13108&radic&radic&radic S000002186MPS1YDL028CverifiedDual-specificity kinase required for spindle pole body (SPB)duplication and spindle checkpoint function;substrates include SPB proteins Spc42p,Spc110p, and Spc98p, mitotic exit networkprotein Mob1p, and checkpoint protein Mad1p 0.002490.13108 S000001112TRM5YHR070WverifiedtRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacentto the anticodon that has a role in preventionof frameshifting; highly conserved acrossArchaea, Bacteria, and Eukarya 0.00250.13108&radic&radic&radic&radic S000000146SEC17YBL050WverifiedPeripheral membrane protein required for vesicular transportbetween ER and Golgi and for the 'priming' stepin homotypic vacuole fusion, part of thecis-SNARE complex; has similarity to alpha-SNAP 0.00250.13108&radic&radic&radic S000000219MNN2YBR015CverifiedAlpha-1,2-mannosyltransferase, responsible for addition of thefirst alpha-1,2-linked mannose to form thebranches on the mannan backbone ofoligosaccharides, localizes to an early Golgicompartment 0.002480.13007&radic S000002470YDR063WUncharacterizedHypothetical protein 0.002480.13007&radic&radic&radic&radic S000002697RTT103YDR289CverifiedProtein that interacts with exonuclease Rat1p and Rai1p andplays a role in transcription termination byRNA polymerase II, has an RPR domain(carboxy-terminal domain interacting domain);also involved in regulation of Ty1transposition 0.002470.13007&radic&radic&radic S000003397MRPS35YGR165WverifiedMitochondrial ribosomal protein of the small subunit 0.002470.13007&radic S000000450YBR246WUncharacterizedHypothetical protein 0.002460.12955&radic&radic&radic S000001596RAD27YKL113Cverified5' to 3' exonuclease, 5' flap endonuclease, required forOkazaki fragment processing and maturation aswell as for long-patch base-excision repair;member of the S. pombe RAD2/FEN1 family 0.002460.12955&radic&radic&radic S000000460RIB5YBR256CverifiedRiboflavin synthase; catalyzes the last step of the riboflavinbiosynthesis pathway 0.002450.12955&radic&radic S000002315YDL156WUncharacterizedHypothetical protein 0.002450.12931&radic&radic&radic S000001501SWD2YKL018WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lys 4 and is involved intelomeric silencing; subunit of CPF (cleavageand polyadenylation factor), a complex involvedin RNAP II transcription termination &radic0.002450.12931&radic&radic&radic&radic S000002874PKH3YDR466WverifiedProtein kinase with similarity to mammalianphosphoinositide-dependent kinase 1 (PDK1) andyeast Pkh1p and Pkh2p, two redundant upstreamactivators of Pkc1p; identified as a multicopysuppressor of a pkh1 pkh2 double mutant 0.002450.12931&radic S000000485DUG2YBR281CuncharacterizedProtein involved in alternative pathway of glutathionedegradation; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmand nucleus; YBR281C is not an essential gene 0.002440.12918&radic&radic&radic S000004434SIR3YLR442CverifiedSilencing protein that interacts with Sir2p and Sir4p, andhistone H3 and H4 tails, to establish atranscriptionally silent chromatin state;required for spreading of silenced chromatin;recruited to chromatin through interaction withRap1p 0.002430.12831&radic S000000867ICL1YER065CverifiedIsocitrate lyase, catalyzes the formation of succinate andglyoxylate from isocitrate, a key reaction ofthe glyoxylate cycle; expression of ICL1 isinduced by growth on ethanol and repressed bygrowth on glucose 0.002430.12831&radic&radic&radic S000000013NTG1YAL015CverifiedDNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involvedin base excision repair, localizes to thenucleus and mitochondrion 0.002420.12831&radic&radic&radic&radic S000000396RIM2YBR192WverifiedMitochondrial pyrimidine nucleotide transporter; importspyrimidine nucleoside triphosphates and exportspyrimidine nucleoside monophosphates; member ofthe mitochondrial carrier family 0.002420.12831&radic&radic&radic&radic S000006339CTF4YPR135WverifiedChromatin-associated protein, required for sister chromatidcohesion; interacts with DNA polymerase alpha(Pol1p) and may link DNA synthesis to sisterchromatid cohesion 0.002430.12831&radic&radic&radic&radic S000001584HSL1YKL101WverifiedNim1p-related protein kinase that regulates the morphogenesisand septin checkpoints; associates with theassembled septin filament; required along withHsl7p for bud neck recruitment,phosphorylation, and degradation of Swe1p 0.002410.12757&radic&radic S000002715YDR307WUncharacterizedHypothetical protein 0.002410.12757&radic&radic S000004632RSF1YMR030WverifiedProtein required for respiratory growth; localized to both thenucleus and mitochondrion; mutant displaysdecreased transcription of specific nuclear andmitochondrial genes whose products are involvedin respiratory growth 0.002420.12757&radic&radic&radic S000000027MYO4YAL029CverifiedOne of two type V myosin motors (along with MYO2) involved inactin-based transport of cargos; required formRNA transport, including ASH1 mRNA, andfacilitating the growth and movement of ERtubules into the growing bud along with She3p 0.002420.12757&radic&radic&radic&radic S000002486PET100YDR079WverifiedChaperone that specifically facilitates the assembly ofcytochrome c oxidase, integral to themitochondrial inner membrane; interacts with asubcomplex of subunits VII, VIIa, and VIII(Cox7p, Cox9p, and Cox8p) but not with theholoenzyme 0.002410.12742&radic&radic&radic&radic S000004909YMR294W-ADubiousHypothetical protein 0.00240.12676&radic&radic S000000174ATG8YBL078CverifiedProtein required for autophagy; modified by the serial actionof Atg4p, Atg7p, and Atg3p, and conjugated atthe C terminus with phosphatidylethanolamine,to become the form essential for generation ofautophagosomes 0.00240.12676&radic&radic S000004852RNT1YMR239CverifiedRNAase III; cleaves a stem-loop structure at the 3' end of U2snRNA to ensure formation of the correct U2 3'end 0.00240.12676&radic&radic&radic&radic&radic S000002437RAD28YDR030CverifiedProtein involved in transcription-coupled repair nucleotideexcision repair of UV-induced DNA lesions;homolog of human CSA protein 0.002380.12622&radic S000000390PCH2YBR186WverifiedNucleolar component of the pachytene checkpoint, which preventschromosome segregation when recombination andchromosome synapsis are defective; alsorepresses meiotic interhomolog recombination inthe rDNA 0.002380.12556&radic&radic S000002520PDS1YDR113CverifiedSecurin that inhibits anaphase by binding separin Esp1p, alsoblocks cyclin destruction and mitotic exit,essential for cell cycle arrest in mitosis inthe presence of DNA damage or aberrant mitoticspindles; also present in meiotic nuclei 0.002380.12556&radic&radic&radic&radic S000004075ARP6YLR085CverifiedActin-related protein that binds nucleosomes; a component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.002370.12556&radic&radic&radic S000003731YJL195CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 0.002380.12556&radic&radic S000001673CNB1YKL190WverifiedCalcineurin B; the regulatory subunit of calcineurin, aCa++/calmodulin-regulated protein phosphatasewhich regulates Crz1p (a stress-responsetranscription factor), the other calcineurinsubunit is encoded by CNA1 and/or CMP1 0.002360.12413&radic&radic&radic S000005051YAF9YNL107WverifiedSubunit of both the NuA4 histone H4 acetyltransferase complexand the SWR1 complex, may function toantagonize silencing near telomeres; interactsdirectly with Swc4p, has homology to humanleukemogenic protein AF9, contains a YEATSdomain 0.002340.12413&radic S000002137YAL034C-BDubiousHypothetical protein 0.002340.1234&radic&radic S000000313CMD1YBR109CverifiedCalmodulin; Ca++ binding protein that regulates Ca++independent processes (mitosis, bud growth,actin organization, endocytosis, etc.) and Ca++dependent processes (stress-activatedpathways), targets include Nuf1p, Myo2p andcalcineurin 0.002340.1234 S000000729GIM4YEL003WverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 0.002340.1234&radic&radic&radic&radic&radic S000006188ACM1YPL267WverifiedCell cycle regulated protein of unknown function; associatedwith Cdh1p and may supress theAPC/C[Cdh1]-mediated proteolysis of mitoticcyclins 0.002290.11993&radic&radic&radic&radic&radic S000006095NIP100YPL174CverifiedLarge subunit of the dynactin complex, which is involved inpartitioning the mitotic spindle between motherand daughter cells; putative ortholog ofmammalian p150(glued) 0.002290.11993 S000001652YKL169CDubiousHypothetical protein 0.00230.11993&radic&radic&radic S000005978SUR1YPL057CverifiedProbable catalytic subunit of a mannosylinositolphosphorylceramide (MIPC) synthase, forms acomplex with probable regulatory subunit Csg2p;function in sphingolipid biosynthesis isoverlapping with that of Csh1p 0.002290.11993&radic&radic&radic S000001594YKL111CDubiousDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1 0.002290.11993&radic S000003798YJR037WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; YJR037W is not an essential gene. 0.002280.11993&radic S000005194RAD50YNL250WverifiedSubunit of MRX complex, with Mre11p and Xrs2p, involved inprocessing double-strand DNA breaks invegetative cells, initiation of meiotic DSBs,telomere maintenance, and nonhomologous endjoining 0.002330.11993&radic&radic S000003451YGR219WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C 0.00230.11993&radic&radic S000000378YBR174CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective 0.002330.11993&radic&radic S000001001YAP3YHL009CverifiedBasic leucine zipper (bZIP) transcription factor 0.00230.11993&radic&radic&radic&radic S000004416SPP382YLR424WverifiedEssential protein that forms a dimer with Ntr2p; also forms atrimer, with Ntr2p and Prp43p, that is involvedin spliceosome disassembly; found also in amultisubunit complex with the splicing factorClf1p; suppressor of prp38-1 mutation 0.002290.11993&radic&radic S000000011DEP1YAL013WverifiedTranscriptional modulator involved in regulation of structuralphospholipid biosynthesis genes andmetabolically unrelated genes, as well asmaintenance of telomeres, mating efficiency,and sporulation 0.002330.11993&radic&radic S000003962UBI4YLL039CverifiedUbiquitin, becomes conjugated to proteins, marking them forselective degradation via the ubiquitin-26Sproteasome system; essential for the cellularstress response 0.002320.11993&radic&radic&radic&radic S000000065RFA1YAR007CverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 0.00230.11993&radic&radic&radic&radic S000005708RPS30BYOR182CverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps30Ap and has similarity to ratS30 ribosomal protein 0.002310.11993&radic&radic&radic S000005365RPB11YOL005CverifiedRNA polymerase II subunit B12.5; part of central core; similarto Rpc19p and bacterial alpha subunit 0.00230.11993&radic&radic&radic S000002539YDR132CUncharacterizedHypothetical protein 0.002280.11993&radic&radic&radic S000000883SER3YER081Wverified3-phosphoglycerate dehydrogenase, catalyzes the first step inserine and glycine biosynthesis; isozyme ofSer33p 0.002280.11993&radic&radic&radic S000006133PUS1YPL212CverifiedtRNA:pseudouridine synthase, introduces pseudouridines atpositions 26-28, 34-36, 65, and 67 of tRNA;nuclear protein that appears to be involved intRNA export; also acts on U2 snRNA 0.002290.11993&radic&radic&radic S000005963SSN3YPL042CverifiedCyclin-dependent protein kinase, component of RNA polymerase IIholoenzyme; involved in phosphorylation of theRNA polymerase II C-terminal domain; involvedin glucose repression 0.002310.11993&radic&radic S000000853JHD1YER051WverifiedJmjC domain family histone demethylase specific for H3-K36,similar to proteins found in human, mouse,drosophila, X. laevis, C. elegans, and S. pombe 0.002290.11993&radic&radic&radic S000003156YGL188CDubiousHypothetical protein 0.002280.11993&radic S000000459YBR255WUncharacterizedProtein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 0.002270.11916&radic&radic S000005097GIM3YNL153CverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 0.002270.11916 S000001056SPO13YHR014WverifiedMeiosis-specific protein, involved in maintaining sisterchromatid cohesion during meiosis I as well aspromoting proper attachment of kinetochores tothe spindle during meiosis I and meiosis II 0.002270.11916&radic&radic S000000832CHZ1YER030WuncharacterizedPutative chaperone for Htz1p/H2A-H2B dimer 0.002270.11916&radic&radic&radic&radic S000000025YAL027WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YAL027W is a non-essential gene 0.002280.11916&radic&radic&radic&radic S000006345KAR3YPR141CverifiedMinus-end-directed microtubule motor that functions in mitosisand meiosis, localizes to the spindle pole bodyand localization is dependent on functionalCik1p, required for nuclear fusion duringmating; potential Cdc28p substrate 0.002270.11916&radic&radic&radic&radic&radic S000002472YDR065WUncharacterizedProtein of unknown function, required for vacuolar acidification 0.002270.11916&radic&radic&radic S000005262HXT14YNL318CverifiedProtein with similarity to hexose transporter family members,expression is induced in low glucose andrepressed in high glucose; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 0.002260.11858&radic&radic&radic&radic&radic S000005222CAF120YNL278WverifiedPart of the evolutionarily-conserved CCR4-NOT transcriptionalregulatory complex involved in controlling mRNAinitiation, elongation, and degradation 0.002250.11813&radic S000004802SGS1YMR190CverifiedNucleolar DNA helicase of the RecQ family involved inmaintenance of genome integrity, regulateschromosome synapsis and meiotic crossing over;has similarity to human BLM and WRN helicasesimplicated in Bloom and Werner syndromes 0.002240.11458&radic&radic&radic&radic&radic S000000105ALK2YBL009WverifiedProtein kinase; accumulation and phosphorylation are periodicduring the cell cycle; phosphorylated inresponse to DNA damage; contains characteristicmotifs for degradation via the APC pathway;similar to Alk1p and to mammalian haspins 0.002210.11458&radic&radic&radic&radic S000000259PRP6YBR055CverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.002210.11458&radic&radic S000000434OM14YBR230CverifiedIntegral mitochondrial outer membrane protein; abundance isdecreased in cells grown in glucose relative toother carbon sources; appears to contain 3alpha-helical transmembrane segments; ORFencodes a 97-basepair intron 0.002220.11458&radic&radic S000004847RNH1YMR234WverifiedRibonuclease H1, removes RNA primers during Okazaki fragmentsynthesis; degrades RNA attached to the 5'-endof a DNA strand 0.002230.11458&radic&radic&radic&radic S000005607TGL5YOR081CverifiedTriacylglycerol lipase involved in TAG mobilization; localizesto lipid particles; potential Cdc28p substrate 0.002240.11458&radic&radic&radic S000002834YDR426CDubiousHypothetical protein 0.00220.11458&radic S000004267YSH1YLR277CverifiedPutative endonuclease, subunit of the mRNA cleavage andpolyadenylation specificity complex requiredfor 3' processing of mRNAs 0.002230.11458&radic&radic S000000111ACH1YBL015WverifiedAcetyl-coA hydrolase, primarily localized to mitochondria;required for acetate utilization and fordiploid pseudohyphal growth 0.002240.11458&radic&radic&radic S000005976LGE1YPL055CverifiedProtein of unknown function; null mutant forms abnormally largecells 0.002240.11458&radic&radic&radic&radic S000001556LHS1YKL073WverifiedMolecular chaperone of the endoplasmic reticulum lumen,involved in polypeptide translocation andfolding; member of the Hsp70 family; localizesto the lumen of the ER; regulated by theunfolded protein response pathway 0.002230.11458 S000001937ERJ5YFR041CuncharacterizedType I membrane protein with a J domain is required to preservethe folding capacity of the endoplasmicreticulum; loss of the non-essential ERJ5 geneleads to a constitutively induced unfoldedprotein response 0.002210.11458&radic&radic&radic S000005705SYC1YOR179CverifiedSubunit of the APT subcomplex of cleavage and polyadenylationfactor, may have a role in 3' end formation ofboth polyadenylated and non-polyadenylated RNAs 0.00220.11458&radic&radic&radic&radic S000000550YCL045CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; interacts with Gal80p; YCL045C is not an essential gene 0.002210.11458&radic&radic&radic S000001158COX23YHR116WverifiedMitochondrial intermembrane space protein that functions inmitochondrial copper homeostasis, essential forfunctional cytochrome oxidase expression;homologous to Cox17p 0.002240.11458&radic&radic&radic S000004735ECM16YMR128WverifiedEssential DEAH-box ATP-dependent RNA helicase specific to theU3 snoRNP, predominantly nucleolar indistribution, required for 18S rRNA synthesis 0.002230.11458 S000004505VPS71YML041Cverifiednucleosome-binding component of the SWR1 complex, whichexchanges histone variant H2AZ (Htz1p) forchromatin-bound histone H2A; required forvacuolar protein sorting 0.002210.11458&radic S000004012SDO1YLR022CuncharacterizedEssential protein involved in RNA metabolism, one of two yeasthomologs (with Yhr087wp) of the human proteinSBDS responsible for autosomal recessiveShwachman-Bodian-Diamond Syndrome, alsoconserved in Archaea 0.002210.11458&radic&radic&radic S000004844PEP5YMR231WverifiedPeripheral vacuolar membrane protein required for proteintrafficking and vacuole biogenesis; formscomplex with Pep3p that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, also interacts with Pep7p 0.002240.11458&radic&radic&radic&radic S000003946YLL023CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLL023C is not an essential gene 0.002210.11458&radic&radic S000004924GAS1YMR307WverifiedBeta-1.3-glucanosyltransferase, required for cell wallassembly; localizes to the cell surface via aglycosylphosphatidylinositol (GPI) anchor 0.00220.11458&radic S000003959PRP19YLL036CverifiedSplicing factor associated with the spliceosome; contains aU-box, a motif found in a class of ubiquitinligases 0.002230.11458&radic&radic&radic&radic S000002607YDR199WDubiousHypothetical protein 0.002240.11458&radic S000001817YAL065CUncharacterizedHypothetical protein 0.002210.11458&radic&radic S000004838MRPL44YMR225CverifiedMitochondrial ribosomal protein of the large subunit 0.002190.11429&radic&radic&radic&radic S000000078PRM9YAR031WverifiedPheromone-regulated protein with 3 predicted transmembranesegments and an FF sequence, a motif involvedin COPII binding; member of DUP240 gene family 0.002190.11334&radic&radic&radic S000005755WTM2YOR229WverifiedTranscriptional repressor involved in regulation of meiosis andsilencing; contains WD repeats 0.002190.11334&radic&radic&radic&radic&radic&radic S000002494RRP1YDR087CverifiedEssential evolutionarily conserved nucleolar protein necessaryfor biogenesis of 60S ribosomal subunits andprocessing of pre-rRNAs to mature rRNAs,associated with several distinct 66Spre-ribosomal particles 0.002190.11334&radic&radic&radic&radic S000002838CYM1YDR430CverifiedLysine-specific metalloprotease of the mitochondrialintermembrane space, member of the pitrilysinfamily; degrades proteins and presequencepeptides cleaved from imported proteins;required for normal mitochondrial morphology 0.002190.11334&radic&radic&radic S000001175NSG1YHR133CverifiedProtein involved in regulation of sterol biosynthesis;specifically stabilizes Hmg2p, one of twoHMG-CoA isoenzymes that catalyze therate-limiting step in sterol biosynthesis;homolog of mammalian INSIG proteins 0.002180.11334&radic&radic&radic&radic S000001243RPN10YHR200WverifiedNon-ATPase base subunit of the 19S regulatory particle (RP) ofthe 26S proteasome; N-terminus plays a role inmaintaining the structural integrity of the RP;binds selectively to polyubiquitin chains;homolog of the mammalian S5a protein 0.002180.11334&radic&radic S000001710PAP1YKR002WverifiedPoly(A) polymerase, one of three factors required for mRNA3'-end polyadenylation, forms multiproteincomplex with polyadenylation factor I (PF I),also required for mRNA nuclear export; may alsopolyadenylate rRNAs 0.002180.11334&radic&radic&radic&radic S000004846YMR233WUncharacterizedProtein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; YMR233W is not an essential gene 0.002190.11334&radic&radic&radic&radic&radic S000000774YEL048CUncharacterizedPutative protein of unknown function; synthetic lethal with gcs1delta 0.002170.11227&radic&radic&radic S000003212TAD1YGL243WverifiedtRNA-specific adenosine deaminase, deaminates adenosine-37 toinosine in tRNA-Ala 0.002160.11227&radic&radic S000000187MAP2YBL091CverifiedMethionine aminopeptidase, catalyzes the cotranslationalremoval of N-terminal methionine from nascentpolypeptides; function is partially redundantwith that of Map1p 0.002160.11227&radic&radic&radic&radic S000004760YIM1YMR152WverifiedProtein of unknown function; null mutant displays sensitivityto DNA damaging agents; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 0.002160.11227&radic&radic&radic S000000491ZSP1YBR287WuncharacterizedProtein of unknown function; mutation results in a zincsensitive phenotype 0.002170.11227&radic S000001049ERG11YHR007CverifiedLanosterol 14-alpha-demethylase, catalyzes the C-14demethylation of lanosterol to form4,4''-dimethylcholesta-8,14,24-triene-3-beta-ol in theergosterol biosynthesis pathway; member of thecytochrome P450 family 0.002170.11227&radic&radic S000004732STO1YMR125WverifiedLarge subunit of the nuclear mRNA cap-binding protein complex,interacts with Npl3p to carry nuclear poly(A)+mRNA to cytoplasm; also involved in nuclearmRNA degradation and telomere maintenance;orthologous to mammalian CBP80 0.002160.11227&radic S000002783BCS1YDR375CverifiedProtein of the mitochondrial inner membrane that functions asan ATP-dependent chaperone, required for theassembly of the cytochrome bc(1) complex fromthe Rip1p and Qcr10p proteins; member of theCDC48/PAS1/SEC18 ATPase family 0.002160.11146&radic&radic S000002535YDR128WUncharacterizedHypothetical protein 0.002160.11146&radic&radic S000000439YBR235WUncharacterizedPutative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene 0.002160.11146&radic&radic&radic&radic S000000640PER1YCR044CverifiedProtein of the endoplasmic reticulum, required forGPI-phospholipase A2 activity that remodels theGPI anchor as a prerequisite for association ofGPI-anchored proteins with lipid rafts;functionally complemented by human orthologPERLD1 0.002150.11146&radic S000003079NSA1YGL111WverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis 0.002150.11146&radic&radic S000002142PAU8YAL068CuncharacterizedHypothetical protein 0.002150.11146&radic&radic&radic S000005377ESC8YOL017WverifiedProtein involved in telomeric and mating-type locus silencing,interacts with Sir2p and also interacts withthe Gal11p, which is a component of the RNA polII mediator complex 0.002140.11028&radic&radic S000006238ARP7YPR034WverifiedActin-related protein involved in transcriptional regulation;subunit of the chromatin remodeling Snf/Swicomplex 0.002130.11028&radic&radic S000004623MAC1YMR021CverifiedCopper-sensing transcription factor involved in regulation ofgenes required for high affinity coppertransport 0.002140.11028&radic&radic&radic&radic S000002725HIM1YDR317WverifiedProtein of unknown function involved in DNA repair 0.002140.11028&radic S000002354SEC31YDL195WverifiedEssential phosphoprotein component (p150) of the COPII coat ofsecretory pathway vesicles, in complex withSec13p; required for ER-derived transportvesicle formation 0.002140.11028&radic S000000066SEN34YAR008WverifiedSubunit of the tRNA splicing endonuclease, which is composed ofSen2p, Sen15p, Sen34p, and Sen54p; Sen34pcontains the active site for tRNA 3' splicesite cleavage and has similarity to Sen2p andto Archaeal tRNA splicing endonuclease 0.002150.11028&radic&radic&radic&radic&radic S000000415AME1YBR211CverifiedEssential kinetochore protein associated with microtubules andspindle pole bodies; component of thekinetochore sub-complex COMA (Ctf19p, Okp1p,Mcm21p, Ame1p); involved in spindle checkpointmaintenance 0.002130.11028&radic&radic&radic S000003693FAR1YJL157CverifiedCyclin-dependent kinase inhibitor that mediates cell cyclearrest in response to pheromone; also forms acomplex with Cdc24p, Ste4p, and Ste18p that mayspecify the direction of polarized growthduring mating; potential Cdc28p substrate 0.002130.11028&radic&radic&radic&radic&radic S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.002130.10925&radic&radic S000002643PRP42YDR235WverifiedU1 snRNP protein involved in splicing, required for U1 snRNPbiogenesis; contains multipletetriatricopeptide repeats 0.002120.10925&radic&radic&radic S000002767VID21YDR359CverifiedComponent of the NuA4 histone acetyltransferase complex 0.002130.10925 S000000798RMD6YEL072WverifiedProtein required for sporulation 0.002120.10925&radic&radic&radic&radic&radic S000000381EHT1YBR177CverifiedAcyl-coenzymeA:ethanol O-acyltransferase that plays a minorrole in medium-chain fatty acid ethyl esterbiosynthesis; contains esterase activity;localizes to lipid particles and themitochondrial outer membrane 0.002130.10925&radic&radic S000006384AOS1YPR180WverifiedNuclear protein that acts as a heterodimer with Uba2p toactivate Smt3p (SUMO) before its conjugation toproteins (sumoylation), which may play a rolein protein targeting; essential for viability 0.002120.10925&radic&radic&radic&radic&radic S000004249HSP60YLR259CverifiedTetradecameric mitochondrial chaperonin required forATP-dependent folding of precursor polypeptidesand complex assembly; prevents aggregation andmediates protein refolding after heat shock;role in mtDNA transmission; similarity to groEL 0.002120.10925&radic&radic S000003769TDH2YJR009CverifiedGlyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involvedin glycolysis and gluconeogenesis; tetramerthat catalyzes the reaction ofglyceraldehyde-3-phosphate to 1,3bis-phosphoglycerate; detected in the cytoplasmand cell-wall 0.002130.10925&radic&radic S000004170SAM1YLR180WverifiedS-adenosylmethionine synthetase, catalyzes transfer of theadenosyl group of ATP to the sulfur atom ofmethionine; one of two differentially regulatedisozymes (Sam1p and Sam2p) 0.002120.10925&radic&radic S000002957TMA20YER007C-AverifiedProtein of unknown function that associates with ribosomes andhas a putative RNA binding domain; interactswith Tma22p; null mutant exhibits translationdefects; has homology to human oncogene MCT-1 0.002120.10925&radic&radic&radic S000005561SHE4YOR035CverifiedProtein containing a UCS (UNC-45/CRO1/SHE4) domain, binds tomyosin motor domains to regulate myosinfunction; involved in endocytosis, polarizationof the actin cytoskeleton, and asymmetric mRNAlocalization 0.002120.10925&radic&radic&radic S000000852RSM18YER050CverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S18 ribosomal protein 0.002130.10925&radic&radic&radic&radic&radic S000004186PWP1YLR196WverifiedProtein with WD-40 repeats involved in rRNA processing;associates with trans-acting ribosomebiogenesis factors; similar to beta-transducinsuperfamily 0.002120.10925&radic&radic&radic S000005952PHO85YPL031CverifiedCyclin-dependent kinase, with ten cyclin partners; involved inenvironmental stress response; inphosphate-rich conditions, Pho85p-Pho80pcomplex phosphorylates Pho4p which in turnrepresses PHO5 0.002110.10923&radic S000005201SIP3YNL257CverifiedProtein that activates transcription through interaction withDNA-bound Snf1p, C-terminal region has aputative leucine zipper motif; potential Cdc28psubstrate 0.002090.10771&radic&radic&radic&radic S000003013RIM8YGL045WverifiedProtein of unknown function, involved in the proteolyticactivation of Rim101p in response to alkalinepH; has similarity to A. nidulans PalF 0.002110.10771&radic&radic&radic S000005647YOR121CDubiousHypothetical protein 0.002110.10771&radic&radic&radic S000000420YBP1YBR216CverifiedProtein required for oxidation of specific cysteine residues ofthe transcription factor Yap1p, resulting inthe nuclear localization of Yap1p in responseto stress 0.002110.10771&radic&radic S000004312MMS22YLR320WverifiedProtein involved in resistance to ionizing radiation; acts withMms1p in a repair pathway that may be involvedin resolving replication intermediates orpreventing the damage caused by blockedreplication forks 0.002110.10771&radic&radic&radic S000001374HOS4YIL112WuncharacterizedSubunit of the Set3 complex, which is a meiotic-specificrepressor of sporulation specific genes thatcontains deacetylase activity; potential Cdc28psubstrate 0.002090.10771&radic&radic S000004073EMP70YLR083CverifiedProtein whose 24kDa cleavage product is found inendosome-enriched membrane fractions, predictedto be a transmembrane protein 0.002110.10771&radic&radic&radic S000001000YHL008CUncharacterizedPutative protein of unknown function, does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 0.002090.10771&radic&radic&radic&radic S000002524TMA64YDR117CverifiedProtein of unknown function that associates with ribosomes; hasa putative RNA binding domain 0.002110.10771&radic&radic&radic S000000740YEL014CDubiousHypothetical protein 0.00210.10771&radic&radic&radic S000004841MTF1YMR228WverifiedMitochondrial RNA polymerase specificity factor with structuralsimilarity to S-adenosylmethionine-dependentmethyltransferases and functional similarity tobacterial sigma-factors, interacts withmitochondrial core polymerase Rpo41p 0.002090.10771&radic&radic S000004234MAP1YLR244CverifiedMethionine aminopeptidase, catalyzes the cotranslationalremoval of N-terminal methionine from nascentpolypeptides; function is partially redundantwith that of Map2p 0.00210.10771&radic S000002178RPN4YDL020CverifiedTranscription factor that stimulates expression of proteasomegenes; Rpn4p levels are in turn regulated bythe 26S proteasome in a negative feedbackcontrol mechanism; RPN4 is transcriptionallyregulated by various stress responses 0.00210.10771&radic S000000521DCC1YCL016CverifiedSubunit of a complex with Ctf8p and Ctf18p that shares somecomponents with Replication Factor C, requiredfor sister chromatid cohesion and telomerelength maintenance 0.002110.10771&radic&radic&radic S000002627MFB1YDR219CverifiedMitochondria-associated F-box protein involved in maintenanceof normal mitochondrial morphology; interactswith Skp1p through the F-box motif;preferentially localizes to the mother cellduring budding 0.00210.10771&radic&radic&radic S000000155YBL059WUncharacterizedHypothetical protein 0.00210.10771&radic&radic&radic&radic S000000824SRB4YER022WverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation 0.00210.10771&radic&radic&radic&radic&radic&radic S000002638YDR230WDubiousHypothetical protein 0.002090.10771&radic&radic S000004043YLR053CUncharacterizedHypothetical protein 0.002090.10771&radic&radic&radic S000001039ARN2YHL047CverifiedTransporter, member of the ARN family of transporters thatspecifically recognize siderophore-ironchelates; responsible for uptake of iron boundto the siderophore triacetylfusarinine C 0.002090.10709&radic&radic&radic&radic S000004929ELP6YMR312WverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;required for Elongator structural integrity 0.002080.10709&radic S000000158YBL062WDubiousHypothetical protein 0.002090.10709&radic&radic S000002771ESC2YDR363WverifiedProtein involved in mating-type locus silencing, interacts withSir2p; probably functions to recruit orstabilize Sir proteins 0.002080.10709&radic&radic&radic S000005835SNU66YOR308CverifiedComponent of the U4/U6.U5 snRNP complex involved in pre-mRNAsplicing via spliceosome; has homology to humanSART-1 and to an S. pombe protein; snu66 nullmutation confers cold-sensitivity but is notlethal at normal growth temperatures 0.002080.10709&radic&radic&radic&radic S000002544RGP1YDR137WverifiedSubunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) thatcatalyzes nucleotide exchange on Ypt6p 0.002080.10684&radic&radic S000001849SEC53YFL045CverifiedPhosphomannomutase, involved in synthesis of GDP-mannose anddolichol-phosphate-mannose; required forfolding and glycosylation of secretory proteinsin the ER lumen 0.002080.10684&radic&radic&radic S000001157DMA1YHR115CverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma2p;homolog of S. pombe Dma1 and H. sapiens Chfr 0.002060.1055&radic&radic&radic S000003303YGR071CUncharacterizedPutative protein of unknown function; deletion mutant has increased glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the nucleus 0.002060.1055&radic&radic&radic S000001171ARP1YHR129CverifiedActin-related protein of the dynactin complex; required forspindle orientation and nuclear migration;putative ortholog of mammalian centractin 0.002060.1055&radic&radic&radic S000001164YHR122WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; may be involved in transcription; YHR122W is an essential gene 0.002060.1055&radic&radic&radic S000003215RAI1YGL246CverifiedNuclear protein that binds to and stabilizes theexoribonuclease Rat1p, required for pre-rRNAprocessing 0.002070.1055&radic S000005229YNL285WDubiousHypothetical protein 0.002060.1055&radic&radic&radic S000004066YLR076CDubiousHypothetical protein; open reading frame overlaps essential RPL10 gene encoding the ribosomal protein L10 which is a component of the large (60S) ribosomal subunit and responsible for joining the large and small subunits 0.002060.1055&radic S000000433ROT2YBR229CverifiedGlucosidase II catalytic subunit required for normal cell wallsynthesis; mutations in rot2 suppress tor2mutations, and are synthetically lethal withrot1 mutations 0.002070.1055&radic S000006160YAR1YPL239WverifiedCytoplasmic ankyrin-repeat containing protein of unknownfunction, proposed to link the processes of 40Sribosomal subunit biogenesis and adaptation toosmotic and oxidative stress; expressionrepressed by heat shock 0.002060.1055&radic&radic&radic&radic S000004470SPT5YML010WverifiedProtein that forms a complex with Spt4p and mediates bothactivation and inhibition of transcriptionelongation; Spt4p-Spt5p complex also plays arole in pre-mRNA processing 0.002060.1055&radic&radic&radic&radic S000003705YJL169WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 0.002070.1055&radic&radic S000004698AIP1YMR092CverifiedActin cortical patch component, interacts with the actindepolymerizing factor cofilin; required torestrict cofilin localization to corticalpatches; contains WD repeats 0.002070.1055&radic&radic S000002548DOP1YDR141CverifiedProtein of unknown function, essential for viability, involvedin establishing cellular polarity andmorphogenesis; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.002060.1055&radic&radic&radic S000004255NEJ1YLR265CverifiedProtein involved in regulation of nonhomologous end joining;repressed by MAT heterozygosity; associateswith Lif1p and regulates its cellulardistribution 0.002070.1055&radic&radic&radic S000000559SPB1YCL054WverifiedAdoMet-dependent methyltransferase involved in rRNA processingand 60S ribosomal subunit maturation;methylates G2922 in the tRNA docking site ofthe large subunit rRNA and in the absence ofsnR52, U2921; suppressor of PAB1 mutants 0.002050.10444&radic&radic&radic S000000252RPS11BYBR048WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps11Ap and has similarity to E.coli S17 and rat S11 ribosomal proteins 0.002040.10444&radic S000002205SIT4YDL047WverifiedType 2A-related serine-threonine phosphatase that functions inthe G1/S transition of the mitotic cycle;cytoplasmic and nuclear protein that modulatesfunctions mediated by Pkc1p including cell walland actin cytoskeleton organization 0.002050.10444&radic&radic S000004067FMP25YLR077WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.002050.10444&radic S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.002040.10444&radic&radic&radic S000004849TAF9YMR236WverifiedSubunit (17 kDa) of TFIID and SAGA complexes, involved in RNApolymerase II transcription initiation and inchromatin modification, similar to histone H3 0.002050.10444&radic&radic&radic&radic S000003683YJL147CUncharacterizedMitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene 0.002040.10444&radic&radic S000003570HCA4YJL033WverifiedPutative nucleolar DEAD box RNA helicase; high-copy numbersuppression of a U14 snoRNA processing mutantsuggests an involvement in 18S rRNA synthesis 0.002040.10444&radic S000000831SMB1YER029CverifiedCore Sm protein Sm B; part of heteroheptameric complex (withSmd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm B and Sm B' 0.002040.10444&radic&radic S000005166SSU72YNL222WverifiedTranscription/RNA-processing factor that associates with TFIIBand cleavage/polyadenylation factor Pta1p;exhibits phosphatase activity on serine-5 ofthe RNA polymerase II C-terminal domain;affects start site selection in vivo 0.002040.10404&radic S000000004SSA1YAL005CverifiedATPase involved in protein folding and nuclear localizationsignal (NLS)-directed nuclear transport; memberof heat shock protein 70 (HSP70) family; formsa chaperone complex with Ydj1p; localized tothe nucleus, cytoplasm, and cell wall 0.002040.10404&radic S000000106YBL010CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles 0.002030.10299&radic&radic&radic S000001723YKR015CUncharacterizedPutative protein of unknown function 0.002020.10299&radic&radic&radic S000005530UTP23YOR004WverifiedEssential nucleolar protein that is a component of the SSU(small subunit) processome involved in 40Sribosomal subunit biogenesis; has homology toPINc domain protein Fcf1p, although the PINcdomain of Utp23p is not required for function 0.002010.10299&radic&radic&radic&radic&radic S000003697FMP33YJL161WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.002010.10299 S000001121IRE1YHR079CverifiedSerine-threonine kinase and endoribonuclease; transmembraneprotein that initiates the unfolded proteinresponse signal by regulating synthesis ofHac1p through HAC1 mRNA splicing 0.002020.10299&radic&radic&radic&radic S000001724FMP13YKR016WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.002020.10299&radic&radic&radic S000002331YDL172CDubiousHypothetical protein 0.002010.10299&radic S000001086DOG1YHR044Cverified2-deoxyglucose-6-phosphate phosphatase, similar to Dog2p,member of a family of low molecular weightphosphatases; confers 2-deoxyglucose resistancewhen overexpressed, in vivo substrate has notyet been identified 0.002010.10299&radic&radic&radic S000000070ADE1YAR015WverifiedN-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR)synthetase, required for 'de novo' purinenucleotide biosynthesis; red pigmentaccumulates in mutant cells deprived of adenine 0.002020.10299&radic&radic S000004906GOT1YMR292WverifiedEvolutionarily conserved non-essential protein present in earlyGolgi cisternae that may be involved inER-Golgi transport at a step after vesicletethering to Golgi membranes, exhibits membranetopology similar to that of Sft2p 0.002020.10299&radic&radic&radic S000005562PEP12YOR036WverifiedTarget membrane receptor (t-SNARE) for vesicular intermediatestraveling between the Golgi apparatus and thevacuole; controls entry of biosynthetic,endocytic, and retrograde traffic into theprevacuolar compartment; syntaxin 0.002020.10299&radic&radic&radic S000000815PRP22YER013WverifiedDEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase,associates with lariat intermediates before thesecond catalytic step of splicing; mediatesATP-dependent mRNA release from the spliceosomeand unwinds RNA duplexes 0.002020.10299&radic&radic S000005573STD1YOR047CverifiedProtein involved in control of glucose-regulated geneexpression; interacts with protein kinaseSnf1p, glucose sensors Snf3p and Rgt2p, andTATA-binding protein Spt15p; acts as aregulator of the transcription factor Rgt1p 0.002030.10299&radic&radic&radic S000006168YPL247CUncharacterizedHypothetical protein 0.0020.10076&radic&radic&radic&radic&radic S000000406CDC47YBR202WverifiedComponent of the hexameric MCM complex, which is important forpriming origins of DNA replication in G1 andbecomes an active ATP-dependent helicase thatpromotes DNA melting and elongation whenactivated by Cdc7p-Dbf4p in S-phase 0.001980.10076&radic&radic&radic S000000909GLE2YER107CverifiedComponent of the nuclear pore complex required forpolyadenylated RNA export but not for proteinimport, homologous to S. pombe Rae1p 0.002010.10076&radic S000002460YDR053WDubiousPutative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex 0.002010.10076&radic&radic&radic S000005242CLA4YNL298WverifiedCdc42p activated signal transducing kinase of the PAK(p21-activated kinase) family, involved inseptin ring assembly and cytokinesis; directlyphosphorylates septins Cdc3p and Cdc10p; otheryeast PAK family members are Ste20p and Skm1p 0.002010.10076&radic&radic&radic S000000048OAF1YAL051WverifiedOleate-activated transcription factor, acts alone and as aheterodimer with Pip2p; activates genesinvolved in beta-oxidation of fatty acids andperoxisome organization and biogenesis 0.001990.10076&radic&radic&radic S000005922HAT1YPL001WverifiedCatalytic subunit of the Hat1p-Hat2p histone acetyltransferasecomplex that uses the cofactor acetyl coenzymeA, to acetylate free nuclear and cytoplasmichistone H4; involved in telomeric silencing andDNA double-strand break repair 0.002010.10076&radic&radic S000005429HST1YOL068CverifiedNAD(+)-dependent histone deacetylase; essential subunit of theSum1p/Rfm1p/Hst1p complex required forORC-dependent silencing and mitotic repression;non-essential subunit of the Set3C deacetylasecomplex; involved in telomere maintenance 0.001990.10076&radic&radic&radic S000004765TPP1YMR156CverifiedDNA 3'-phosphatase that functions in repair of endogenousdamage of double-stranded DNA, activity isspecific for removal of 3' phosphates at strandbreaks; has similarity to the l-2-haloaciddehalogenase superfamily 0.001980.10076&radic&radic&radic S000002824SYF1YDR416WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression; similar to Drosophila crooked neckprotein 0.0020.10076&radic&radic&radic S000003954GPI13YLL031CverifiedER membrane localized phosphoryltransferase that addsphosphoethanolamine onto the third mannoseresidue of the glycosylphosphatidylinositol(GPI) anchor precursor; similar to human PIG-Oprotein 0.001990.10076&radic S000002540YDR133CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C 0.001990.10076&radic&radic S000001681PTK1YKL198CverifiedPutative serine/threonine protein kinase that regulatesspermine uptake; involved in polyaminetransport; possible mitochondrial protein 0.001990.10076&radic&radic&radic S000006402SGE1YPR198WverifiedMembrane-associated multidrug transporter, acts as an extrusionpermease, member of the drug-resistance proteinfamily within the major facilitator superfamily(MFS), partial multicopy suppressor of gal11mutations 0.001990.10076&radic&radic&radic S000000742NPP2YEL016CverifiedNucleotide pyrophosphatase/phosphodiesterase family member;mediates extracellular nucleotide phosphatehydrolysis along with Npp1p and Pho5p; activityand expression enhanced during conditions ofphosphate starvation 0.001990.10076&radic&radic&radic S000002309RPC53YDL150WverifiedRNA polymerase III subunit C53 0.002010.10076&radic&radic S000003274YGR042WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 0.0020.10076&radic&radic&radic&radic S000003957RIX7YLL034CverifiedPutative ATPase of the AAA family, required for export ofpre-ribosomal large subunits from the nucleus;distributed between the nucleolus, nucleoplasm,and nuclear periphery depending on growthconditions 0.0020.10076&radic&radic&radic&radic S000002474OCA6YDR067CuncharacterizedCytoplasmic protein required for replication of Brome mosaicvirus in S. cerevisiae, which is a model systemfor studying replication of positive-strand RNAviruses in their natural hosts 0.002010.10076&radic&radic&radic S000001863HAC1YFL031WverifiedbZIP transcription factor (ATF/CREB1 homolog) that regulatesthe unfolded protein response, via UPREbinding, and membrane biogenesis; ERstress-induced splicing pathway utilizingIre1p, Trl1p and Ada5p facilitates efficientHac1p synthesis 0.001980.10076&radic&radic&radic&radic S000000981DMC1YER179WverifiedMeiosis-specific protein required for repair of double-strandbreaks and pairing between homologouschromosomes; homolog of Rad51p and thebacterial RecA protein 0.001980.10076 S000004887RCE1YMR274CverifiedType II CAAX prenyl protease involved in the proteolysis andmaturation of Ras and the a-factor matingpheromone 0.002010.10076&radic S000001493UFD4YKL010CverifiedUbiquitin-protein ligase (E3) that interacts with Rpt4p andRpt6p, two subunits of the 19S particle of the26S proteasome; cytoplasmic E3 involved in thedegradation of ubiquitin fusion proteins 0.001990.10076&radic&radic&radic S000005510YOL150CDubiousHypothetical protein 0.001990.10076&radic S000006403ARR1YPR199CverifiedTranscriptional activator of the bZIP family, required fortranscription of genes involved in resistanceto arsenic compounds 0.0020.10076&radic&radic S000002127SBH2YER019C-AverifiedSsh1p-Sss1p-Sbh2p complex component, involved in proteintranslocation into the endoplasmic reticulum;homologous to Sbh1p 0.001970.10064&radic&radic&radic S000003207CSE1YGL238WverifiedNuclear envelope protein that mediates the nuclear export ofimportin alpha (Srp1p), homolog of metazoan CASprotein, required for accurate chromosomesegregation 0.001970.10064&radic&radic S000002732UTP4YDR324CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.001970.10064&radic&radic S000003925RTT109YLL002WverifiedProtein with a role in regulation of Ty1 transposition 0.001950.09903&radic&radic&radic&radic S000000368ARL1YBR164CverifiedSoluble GTPase with a role in regulation of membrane traffic;regulates potassium influx; G protein of theRas superfamily, similar to ADP-ribosylationfactor 0.001960.09903&radic S000004461YML002WUncharacterizedHypothetical protein 0.001970.09903&radic&radic&radic&radic S000002556YDR149CDubiousHypothetical protein 0.001950.09903&radic&radic S000005498YOL138CUncharacterizedHypothetical protein 0.001950.09903&radic&radic S000001316YIL054WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data 0.001960.09903 S000003941DPS1YLL018CverifiedCytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme thatcatalyzes the specific aspartylation oftRNA(Asp); class II aminoacyl tRNA synthetase;binding to its own mRNA may conferautoregulation 0.001960.09903&radic&radic&radic S000002254OPI6YDL096CdubiousDubious open reading frame unlikely to encode a protein, basedon available experimental and comparativesequence data; partially overlaps verified genePMT1/YDL095W; YDL096C is not essential 0.001960.09903 S000005405PSK2YOL045WverifiedOne of two (see also PSK1) PAS domain containing S/T proteinkinases; regulates sugar flux and translationin response to an unknown metabolite byphosphorylating Ugp1p and Gsy2p (sugar flux)and Caf20p, Tif11p and Sro9p (translation) 0.001950.09903&radic&radic S000000871ARG5,6YER069WverifiedProtein that is processed in the mitochondrion to yieldacetylglutamate kinase andN-acetyl-gamma-glutamyl-phosphate reductase,which catalyze the 2nd and 3rd steps inarginine biosynthesis; enzymes form a complexwith Arg2p 0.001960.09903&radic&radic S000003718YJL182CDubiousHypothetical protein 0.001970.09903 S000001348YIL086CDubiousHypothetical protein 0.001970.09903&radic&radic&radic S000001052RPL27AYHR010WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl27Bp and has similarity to ratL27 ribosomal protein 0.001960.09903&radic&radic&radic S000002542YCF1YDR135CverifiedVacuolar glutathione S-conjugate transporter of the ATP-bindingcassette family, has a role in detoxifyingmetals such as cadmium, mercury, and arsenite;also transports unconjugated bilirubin; similarto human cystic fibrosis protein CFTR 0.001960.09903&radic&radic S000002709CFT1YDR301WverifiedRNA-binding subunit of the mRNA cleavage and polyadenylationfactor; involved in poly(A) site recognitionand required for both pre-mRNA cleavage andpolyadenylation, 51% sequence similarity withmammalian AAUAA-binding subunit of CPSF 0.001940.09879&radic&radic S000002835RPN9YDR427WverifiedNon-ATPase regulatory subunit of the 26S proteasome, hassimilarity to putative proteasomal subunits inother species; null mutant is temperaturesensitive and exhibits cell cycle andproteasome assembly defects 0.001940.09879&radic S000002988GET1YGL020CverifiedSubunit of the GET complex; required for the retrieval of HDELproteins from the Golgi to the ER in an ERD2dependent fashion and for normal mitochondrialmorphology and inheritance 0.001940.09879 S000000816HEM14YER014WverifiedProtoporphyrinogen oxidase, a mitochondrial enzyme thatcatalyzes the seventh step in the hemebiosynthetic pathway, convertingprotoporphyrinogen IX to protoporphyrin IX 0.001940.09879&radic&radic&radic S000005238RIM21YNL294CverifiedComponent of the RIM101 pathway, has a role in cell wallconstruction and alkaline pH response; hassimilarity to A. nidulans PalH 0.001930.09697&radic&radic&radic S000002528DPB4YDR121WverifiedShared subunit of DNA polymerase epsilon and of ISW2/yCHRACchromatin accessibility complex; involved inboth chromosomal DNA replication and ininheritance of telomeric silencing 0.001930.09697&radic&radic&radic S000001745SPC34YKR037CverifiedEssential subunit of the Dam1 complex (aka DASH complex),couples kinetochores to the force produced byMT depolymerization thereby aiding inchromosome segregation; also localized tonuclear side of spindle pole body 0.001940.09697&radic&radic&radic&radic S000002766GGA1YDR358WverifiedGolgi-localized protein with homology to gamma-adaptin,interacts with and regulates Arf1p and Arf2p ina GTP-dependent manner in order to facilitatetraffic through the late Golgi 0.001930.09697&radic&radic S000001789RPF2YKR081CverifiedEssential protein involved in the processing of pre-rRNA andthe assembly of the 60S ribosomal subunit;interacts with ribosomal protein L11; localizespredominantly to the nucleolus; constituent of66S pre-ribosomal particles 0.001910.09697&radic S000002594YDR186CUncharacterizedHypothetical protein 0.001910.09697&radic&radic S000004016SED5YLR026Cverifiedcis-Golgi t-SNARE syntaxin required for vesicular transportbetween the ER and the Golgi complex, binds atleast 9 SNARE proteins 0.001920.09697&radic&radic S000001550YNK1YKL067WverifiedNucleoside diphosphate kinase, catalyzes the transfer of gammaphosphates from nucleoside triphosphates,usually ATP, to nucleoside diphosphates by amechanism that involves formation of anautophosphorylated enzyme intermediate 0.001920.09697&radic&radic&radic&radic S000002782YDR374CUncharacterizedHypothetical protein 0.001920.09697&radic S000002841YDR433WDubiousHypothetical protein 0.001920.09697&radic S000004680RCO1YMR075WverifiedEssential subunit of the histone deacetylase Rpd3S complex;interacts with Eaf3p 0.001930.09697&radic&radic S000001334HOP1YIL072WverifiedMeiosis-specific DNA binding protein that displays Red1pdependent localization to the unsynapsedaxial-lateral elements of the synaptonemalcomplex; required for homologous chromosomesynapsis and chiasma formation 0.001920.09697&radic&radic S000002508ARX1YDR101CverifiedShuttling pre-60S factor; involved in the biogenesis ofribosomal large subunit biogenesis; interactsdirectly with Alb1; responsible for Tif6recycling defects in absence of Rei1;associated with the ribosomal export complex 0.001930.09697&radic&radic S000000752SNU13YEL026WverifiedRNA binding protein, part of U3 snoRNP involved in rRNAprocessing, part of U4/U6-U5 tri-snRNP involvedin mRNA splicing, similar to human 15.5Kprotein 0.001930.09697&radic&radic&radic S000005266KRE1YNL322CverifiedCell wall glycoprotein involved in beta-glucan assembly; servesas a K1 killer toxin membrane receptor 0.001930.09697&radic&radic&radic S000005193MPA43YNL249CverifiedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.001920.09697&radic&radic&radic&radic S000004273YLR283WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene 0.001910.09697&radic&radic&radic&radic S000002251PMT5YDL093WverifiedProtein O-mannosyltransferase, transfers mannose residues fromdolichyl phosphate-D-mannose to proteinserine/threonine residues; acts in a complexwith Pmt3p, can instead interact with Pmt2p insome conditions; target for new antifungals 0.001930.09697 S000005651CAT5YOR125CverifiedMitochondrial inner membrane protein directly involved inubiquinone biosynthesis, essential for severalother metabolic pathways including respirationand gluconeogenic gene activation 0.001920.09697 S000000165AST1YBL069WverifiedPeripheral membrane protein that interacts with the plasmamembrane ATPase Pma1p and has a role in itstargeting to the plasma membrane, possibly byinfluencing its incorporation into lipid rafts 0.001930.09697&radic&radic&radic&radic&radic S000000995LAG1YHL003CverifiedCeramide synthase component, involved in synthesis of ceramidefrom C26(acyl)-coenzyme A anddihydrosphingosine or phytosphingosine,functionally equivalent to Lac1p 0.001930.09697&radic&radic&radic S000004876SAP30YMR263WverifiedSubunit of a histone deacetylase complex, along with Rpd3p andSin3p, that is involved in silencing attelomeres, rDNA, and silent mating-type loci;involved in telomere maintenance 0.001920.09697&radic&radic&radic S000005241MON2YNL297CverifiedPeripheral membrane protein with a role in endocytosis andvacuole integrity, interacts with Arl1p andlocalizes to the endosome; member of the Sec7pfamily of proteins 0.001920.09697&radic&radic S000002572TRM82YDR165WverifiedSubunit of a tRNA methyltransferase complex composed of Trm8pand Trm82p that catalyzes 7-methylguanosinemodification of tRNA 0.00190.09561&radic&radic&radic&radic S000002592ATC1YDR184CverifiedNuclear protein, possibly involved in regulation of cationstress responses and/or in the establishment ofbipolar budding pattern 0.00190.09561&radic&radic S000005632VAM3YOR106WverifiedSyntaxin-related protein required for vacuolar assembly;functions with Vam7p in vacuolar proteintrafficking; member of the syntaxin family ofproteins 0.001890.09561&radic&radic S000002521YDR114CUncharacterizedPutative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium 0.00190.09561&radic&radic S000004395SFP1YLR403WverifiedTranscription factor that controls expression of many ribosomebiogenesis genes in response to nutrients andstress, regulates G2/M transitions duringmitotic cell cycle and DNA-damage response,involved in cell size modulation 0.00190.09561&radic&radic S000004571NUP188YML103CverifiedSubunit of the nuclear pore complex (NPC), involved in thestructural organization of the complex and ofthe nuclear envelope, also involved in nuclearenvelope permeability, interacts with Pom152pand Nic96p 0.001910.09561 S000000444THI2YBR240CverifiedZinc finger protein of the Zn(II)2Cys6 type, probabletranscriptional activator of thiaminebiosynthetic genes 0.00190.09561&radic&radic&radic S000005563CYC2YOR037WverifiedMitochondrial peripheral inner membrane protein, contains a FADcofactor in a domain exposed in theintermembrane space; exhibits redox activity invitro; likely participates in ligation of hemeto acytochromes c and c1 (Cyc1p and Cyt1p) 0.00190.09561&radic&radic&radic S000004980YNL035CUncharacterizedPutative protein of unknown function with similarity to proteins containing WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene 0.00190.09561&radic&radic S000002722RAD34YDR314CverifiedProtein involved in nucleotide excision repair (NER);homologous to RAD4 0.00190.09561&radic&radic S000003201TAN1YGL232WverifiedPutative tRNA acetyltransferase, RNA-binding protein requiredfor the formation of the modified nucleosideN(4)-acetylcytidine in serine and leucine tRNAsbut not required for the same modification in18S rRNA 0.001890.09561&radic&radic&radic S000005155YNL211CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene 0.001890.09561&radic&radic&radic S000002778YDR370CUncharacterizedHypothetical protein 0.001890.09561&radic&radic S000001070DAP2YHR028CverifiedDipeptidyl aminopeptidase, synthesized as a glycosylatedprecursor; localizes to the vacuolar membrane;similar to Ste13p 0.001880.09511&radic&radic S000004824DML1YMR211WverifiedEssential protein involved in mtDNA inheritance, may alsofunction in the partitioning of themitochondrial organelle or in the segregationof chromosomes, exhibits regions similar tomembers of a GTPase family 0.001880.09511&radic&radic&radic S000007235SEM1YDR363W-AverifiedComponent of the lid subcomplex of the regulatory subunit ofthe 26S proteasome; ortholog of human DSS1 0.001880.09511&radic S000002785ATP17YDR377WverifiedSubunit f of the F0 sector of mitochondrial F1F0 ATP synthase,which is a large, evolutionarily conservedenzyme complex required for ATP synthesis 0.001880.09511&radic&radic&radic S000006099CBC2YPL178WverifiedSmall subunit of the heterodimeric cap binding complex thatalso contains Sto1p, component of thespliceosomal commitment complex; interacts withNpl3p, possibly to package mRNA for export fromthe nucleus; contains an RNA-binding motif 0.001880.09511&radic&radic&radic S000003046DBP3YGL078CverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in ribosomal biogenesis 0.001880.09511&radic&radic&radic S000003459DIE2YGR227WverifiedDolichyl-phosphoglucose-dependent glucosyltransferase of theER, functions in the dolichol pathway thatsynthesizes the dolichol-linked oligosaccharideprecursor for N-linked protein glycosylation,has a role in regulation of ITR1 and INO1 0.001880.09511&radic&radic&radic S000000463YBR259WUncharacterizedPutative protein of unknown function; YBR259W is not an essential gene 0.001880.09511&radic&radic&radic&radic S000005823TIM18YOR297CverifiedComponent of the mitochondrial Tim54p-Tim22p complex involvedin insertion of polytopic proteins into theinner membrane; may function to stabilize thecomplex 0.001880.09511&radic&radic&radic S000003956IRC19YLL033WuncharacterizedPutative protein of unknown function; YLL033W is not anessential gene but mutant is defective in sporeformation; null mutant displays increasedlevels of spontaneous Rad52 foci 0.001880.09511&radic&radic S000004208YLR218CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR218C is not essential; mutants exhibit glycogen storage defects and growth defects on a non-fermentable carbon source 0.001880.09511&radic&radic&radic S000005567YOR041CDubiousDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance 0.001880.09511&radic&radic&radic S000001730NTR2YKR022CverifiedEssential protein that forms a dimer with Ntr1p; also forms atrimer, with Ntr2p and the DExD/H-box RNAhelicase Prp43p, that is involved inspliceosome disassembly 0.001880.09511&radic&radic&radic&radic&radic S000003399CLC1YGR167WverifiedClathrin light chain, subunit of the major coat proteininvolved in intracellular protein transport andendocytosis; thought to regulate clathrinfunction, two Clathrin heavy chains (CHC1) formthe clathrin triskelion structural component 0.001860.09415&radic&radic&radic&radic S000003563RNR2YJL026WverifiedRibonucleotide-diphosphate reductase (RNR), small subunit; theRNR complex catalyzes the rate-limiting step indNTP synthesis and is regulated by DNAreplication and DNA damage checkpoint pathwaysvia localization of the small subunits 0.001870.09415&radic&radic&radic&radic&radic S000005784HNT3YOR258WverifiedMember of the third branch of the histidine triad (HIT)superfamily of nucleotide-binding proteins;similar to Aprataxin, a Hint related proteinthat is mutated in individuals with ataxia withoculomotor apraxia 0.001870.09415&radic&radic S000003358YGR126WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 0.001860.09415&radic&radic S000000392NTC20YBR188CverifiedMember of a complex, including Prp19p, that binds to thespliceosome; required for pre-mRNA splicing 0.001870.09415&radic&radic&radic S000000849SAP1YER047CverifiedPutative ATPase of the AAA family, interacts with the Sin1ptranscriptional repressor in the two-hybridsystem 0.001870.09415&radic&radic S000004631FAR8YMR029CverifiedProtein involved in G1 cell cycle arrest in response topheromone, in a pathway different from theFar1p-dependent pathway; interacts with Far3p,Far7p, Far9p, Far10p, and Far11p 0.001860.09415 S000004873TIF11YMR260CverifiedTranslation initiation factor eIF1A, essential protein thatforms a complex with Sui1p (eIF1) and the 40Sribosomal subunit and scans for the startcodon; C-terminus associates with Fun12p(eIF5B); N terminus interacts with eIF2 andeIF3 0.001850.09324&radic&radic&radic S000000819AFG3YER017CverifiedComponent, with Yta12p, of the mitochondrial inner membranem-AAA protease that mediates degradation ofmisfolded or unassembled proteins and is alsorequired for correct assembly of mitochondrialenzyme complexes 0.001850.09324&radic&radic&radic&radic&radic S000002649BUD26YDR241WdubiousDubious open reading frame, unlikely to encode a protein; notconserved in closely related Saccharomycesspecies; 1% of ORF overlaps the verified geneSNU56; diploid mutant displays a weak buddingpattern phenotype in a systematic assay 0.001850.09324&radic&radic&radic&radic S000000966CHD1YER164WverifiedNucleosome remodeling factor that functions in regulation oftranscription elongation; contains a chromodomain, a helicase domain and a DNA-bindingdomain; component of both the SAGA and SILKcomplexes 0.001840.09324&radic S000005596GYP1YOR070CverifiedCis-golgi GTPase-activating protein (GAP) for the Rab familymembers Ypt1p (in vivo) and for Ypt1p, Sec4p,Ypt7p, and Ypt51p (in vitro); involved invesicle docking and fusion 0.001850.09324&radic&radic&radic S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif 0.001850.09324&radic&radic&radic&radic S000004216BUR2YLR226WverifiedCyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2pcomprise a CDK-cyclin complex involved intranscriptional regulation through itsphosphorylation of the carboxy-terminal domainof the largest subunit of RNA polymerase II 0.001840.09324&radic&radic&radic S000003506TAF1YGR274CverifiedTFIID subunit (145 kDa), involved in RNA polymerase IItranscription initiation, has histoneacetyltransferase activity, involved inpromoter binding and G1/S progression 0.001840.09324&radic&radic&radic S000006182YPL261CDubiousDubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W 0.001850.09324&radic&radic S000003938BPT1YLL015WverifiedABC type transmembrane transporter of MRP/CFTR family, found invacuolar membrane, involved in the transport ofunconjugated bilirubin and in heavy metaldetoxification via glutathione conjugates,along with Ycf1p 0.001850.09324&radic&radic&radic S000001359FYV10YIL097WverifiedProtein of unknown function, required for survival uponexposure to K1 killer toxin; involved inproteasome-dependent catabolite inactivation offructose-1,6-bisphosphatase; contains CTLHdomain 0.001850.09324&radic&radic&radic&radic S000002323CDC9YDL164CverifiedDNA ligase found in the nucleus and mitochondria, an essentialenzyme that joins Okazaki fragments during DNAreplication; also acts in nucleotide excisionrepair, base excision repair, and recombination 0.001850.09324&radic&radic S000001311DFG10YIL049WverifiedProtein of unknown function, involved in filamentous growth 0.001850.09324&radic S000004872YMR259CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR259C is not an essential gene 0.001850.09324&radic&radic S000000161YBL065WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene 0.001840.09324&radic&radic S000003093MET13YGL125WverifiedIsozyme of methylenetetrahydrofolate reductase, catalyzes thereduction of 5,10-methylenetetrahydrofolate to5-methyltetrahydrofolate in the methioninebiosynthesis pathway 0.001840.09324&radic&radic S000000998SHU1YHL006CuncharacterizedProtein of unassigned function involved in mutationsuppression, important for error-free repair ofspontaneous and induced DNA lesions to protectthe genome from mutation; associates withShu2p, Psy3p, and Csm2p 0.001850.09324&radic&radic&radic S000002249UBX3YDL091CuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p, green fluorescentprotein (GFP)-fusion protein localizes to thecytoplasm in a punctate pattern 0.001840.09324&radic S000004158YLR168CUncharacterizedPutative protein of unknown function that may be involved in intramitochondrial sorting; has similarity to Ups1p and to human PRELI; the green fluorescent protein (GFP)-tagged protein localizes to mitochondria 0.001830.09144&radic S000001367SLM1YIL105CverifiedPhosphoinositide PI4,5P(2) binding protein, forms a complexwith Slm2p; acts downstream of Mss4p in apathway regulating actin cytoskeletonorganization in response to stress; subunit ofand phosphorylated by the TORC2 complex 0.001840.09144 S000004284GSP1YLR293CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; regulated by Prp20p,Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p andKap95p; yeast Gsp2p homolog 0.001830.09144&radic&radic&radic S000006390PZF1YPR186CverifiedTranscription factor IIIA (TFIIIA), essential protein with nineC2H2 Zn-fingers, binds the 5S rRNA gene throughthe zinc finger domain and directs assembly ofa multiprotein initiation complex for RNApolymerase III; also binds DNA 0.001840.09144&radic&radic&radic&radic S000002172NOP1YDL014WverifiedNucleolar protein, component of the small subunit processomecomplex, which is required for processing ofpre-18S rRNA; has similarity to mammalianfibrillarin 0.001830.09144&radic S000001366SHQ1YIL104CverifiedEssential nuclear protein, required for accumulation of boxH/ACA snoRNAs and for rRNA processing;interacts with Naf1p 0.001810.09144&radic S000003271YGR039WDubiousHypothetical protein 0.001820.09144&radic&radic S000003344SHY1YGR112WverifiedMitochondrial inner membrane protein required for normalrespiration, possible chaperone involved inassembly of cytochrome c oxidase; similar toSURF1 from mammals, chickens, and D.melanogaster 0.001830.09144&radic&radic S000000069BUD14YAR014CverifiedProtein involved in bud-site selection, Bud14p-Glc7p complexfunctions as a cortical regulator of dynein;diploid mutants display a random buddingpattern instead of the wild-type bipolarpattern 0.001820.09144&radic&radic&radic S000005208PDR17YNL264CverifiedPhosphatidylinositol transfer protein (PITP), downregulatesPlb1p-mediated turnover of phosphatidylcholine,found in the cytosol and microsomes, homologousto Pdr16p, deletion affects phospholipidcomposition 0.001820.09144&radic&radic&radic&radic&radic S000002495SLU7YDR088CverifiedRNA splicing factor, required for ATP-independent portion of2nd catalytic step of spliceosomal RNAsplicing; interacts with Prp18p; contains zincknuckle domain 0.001810.09144&radic&radic&radic S000002823YDR415CUncharacterizedHypothetical protein 0.001820.09144&radic S000005929CHL1YPL008WverifiedConserved nuclear protein required to establishsister-chromatid pairing during S-phase,probable DNA helicase with similarity to humanBACH1, which associates with tumor suppressorBRCA1; associates with acetyltransferase Ctf7p 0.001830.09144&radic S000003305YGR073CDubiousHypothetical protein; open reading frame extensively overlaps essential SMD1 gene encoding a U6 snRNP protein 0.001830.09144&radic&radic S000004880PPA2YMR267WverifiedMitochondrial inorganic pyrophosphatase, required formitochondrial function and possibly involved inenergy generation from inorganic pyrophosphate 0.001840.09144&radic&radic S000005163ALG9YNL219CverifiedMannosyltransferase, involved in N-linked glycosylation;catalyzes the transfer of mannose fromDol-P-Man to lipid-linked oligosaccharides;mutation of the human ortholog causes type 1congenital disorders of glycosylation 0.001810.09144&radic&radic S000002504MSH6YDR097CverifiedProtein required for mismatch repair in mitosis and meiosis,forms a complex with Msh2p to repair bothsingle-base & insertion-deletion mispairs;potentially phosphorylated by Cdc28p 0.001820.09144&radic&radic&radic S000002822ERD1YDR414CverifiedPredicted membrane protein required for the retention oflumenal endoplasmic reticulum proteins; mutantssecrete the endogenous ER protein, BiP (Kar2p) 0.001810.09144&radic&radic&radic S000002989ALK1YGL021WverifiedProtein kinase; accumulation and phosphorylation are periodicduring the cell cycle; phosphorylated inresponse to DNA damage; contains characteristicmotifs for degradation via the APC pathway;similar to Alk2p and to mammalian haspins 0.001830.09144&radic&radic S000000003EFB1YAL003WverifiedTranslation elongation factor 1 beta; stimulates nucleotideexchange to regenerate EF-1 alpha-GTP for thenext elongation cycle; part of the EF-1complex, which facilitates binding ofaminoacyl-tRNA to the ribosomal A site 0.001810.09144&radic&radic S000003016RPT6YGL048CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; bound byubiquitin-protein ligases Ubr1p and Ufd4p;localized mainly to the nucleus throughout thecell cycle 0.001810.09144&radic&radic&radic S000005735NPT1YOR209CverifiedNicotinate phosphoribosyltransferase, acts in the salvagepathway of NAD+ biosynthesis; required forsilencing at rDNA and telomeres and has a rolein silencing at mating-type loci; localized tothe nucleus 0.001830.09144&radic&radic S000004886ZDS1YMR273CverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; has arole in localization of Bcy1p, a regulatorysubunit of protein kinase A; implicated in mRNAnuclear export 0.001840.09144 S000001886SMC1YFL008WverifiedSubunit of the multiprotein cohesin complex, essential proteininvolved in chromosome segregation and indouble-strand DNA break repair; SMC chromosomalATPase family member, binds DNA with apreference for DNA with secondary structure 0.001840.09144&radic&radic S000004029RIC1YLR039CverifiedProtein involved in retrograde transport to the cis-Golginetwork; forms heterodimer with Rgp1p that actsas a GTP exchange factor for Ypt6p; involved intranscription of rRNA and ribosomal proteingenes 0.001810.09144 S000003125YGL157WVerifiedOxidoreductase, catalyzes NADPH-dependent reduction of the bicyclic diketone bicyclo[2.2.2]octane-2,6-dione (BCO2,6D) to the chiral ketoalcohol (1R,4S,6S)-6-hydroxybicyclo[2.2.2]octane-2-one (BCO2one6ol) 0.001810.09144&radic&radic S000002984KAP122YGL016WverifiedKaryopherin beta, responsible for import of the Toa1p-Toa2pcomplex into the nucleus; binds to nucleoporinsNup1p and Nup2p; may play a role in regulationof pleiotropic drug resistance 0.001820.09144&radic&radic&radic S000002693ZIP1YDR285WverifiedTransverse filament protein of the synaptonemal complex;required for normal levels of meioticrecombination and pairing between homologouschromosome during meiosis; potential Cdc28psubstrate 0.001810.09144&radic&radic S000006347RRP15YPR143WverifiedNucleolar protein, constituent of pre-60S ribosomal particles;required for processing of the 27S pre-rRNA atthe A2 site to yield 5.8S and 25S rRNA 0.001820.09144&radic&radic S000003256THG1YGR024CverifiedtRNAHis guanylyltransferase, adds a guanosine residue to the 5'end of tRNAHis after transcription and RNase Pcleavage; couples nuclear division andmigration to cell budding and cytokinesis;essential enzyme conserved among eukaryotes 0.001820.09144&radic S000006063YPL142CDubiousHypothetical protein 0.001820.09144&radic&radic S000004668AEP1YMR064WverifiedProtein required for expression of the mitochondrial OLI1 geneencoding subunit 9 of F1-F0 ATP synthase 0.001820.09144&radic S000004656FAR3YMR052WverifiedProtein involved in G1 cell cycle arrest in response topheromone, in a pathway different from theFar1p-dependent pathway; interacts with Far7p,Far8p, Far9p, Far10p, and Far11p 0.001830.09144&radic S000002562CPR1YDR155CverifiedCytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin),catalyzes the cis-trans isomerization ofpeptide bonds N-terminal to proline residues;binds the drug cyclosporin A 0.00180.09069&radic&radic&radic&radic S000000015PSK1YAL017WverifiedOne of two (see also PSK2) PAS domain containing S/T proteinkinases; coordinately regulates proteinsynthesis and carbohydrate metabolism andstorage in response to a unknown metabolitethat reflects nutritional status 0.00180.09069&radic&radic&radic S000000617YCR023CUncharacterizedPutative protein of unknown function; YCR023C is not an essential gene 0.00180.09069&radic&radic&radic S000001153UBA4YHR111WverifiedProtein that activates Urm1p before its conjugation to proteins(urmylation); one target is the thioredoxinperoxidase Ahp1p, suggesting a role ofurmylation in the oxidative stress response 0.00180.09069&radic&radic S000000513YCL007CDubiousDubious ORF unlikely to encode a protein; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A 0.00180.09069&radic&radic&radic S000000131POL12YBL035CverifiedB subunit of DNA polymerase alpha-primase complex, required forinitiation of DNA replication during mitoticand premeiotic DNA synthesis; also functions intelomere capping and length regulation 0.00180.09069&radic&radic&radic&radic S000000870MOT2YER068WverifiedComponent of the CCR4-NOT complex, which has multiple roles intranscription regulation, mRNA degradation, andpost-transcriptional modifications; with Ubc4p,ubiquitylates subunits of the nascentpolypeptide-associated complex (NAC) 0.00180.09069&radic&radic&radic S000003383YGR151CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C 0.00180.09069&radic&radic&radic S000006119RPL7BYPL198WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl7Ap and has similarity to E.coli L30 and rat L7 ribosomal proteins;contains a conserved C-terminal Nucleic acidBinding Domain (NDB2) 0.00180.09069&radic&radic S000002716SRB7YDR308CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation; target of theglobal repressor Tup1p 0.00180.09069&radic&radic&radic&radic S000003018TYW3YGL050WuncharacterizedHypothetical protein 0.00180.09069&radic&radic&radic S000000560KAR4YCL055WverifiedTranscription factor required for gene regulation in repsonseto pheromones; also required during meiosis;exists in two forms, a slower-migrating formmore abundant during vegetative growth and afaster-migrating form induced by pheromone 0.00180.09069&radic&radic S000001312PCL7YIL050WverifiedPho85p cyclin of the Pho80p subfamily, forms a functionalkinase complex with Pho85p which phosphorylatesMmr1p and is regulated by Pho81p; involved inglycogen metabolism, expression is cell-cycleregulated 0.001810.09069&radic S000004280GUF1YLR289WverifiedMitochondrial GTPase of unknown function, similar to E. colielongation factor-type GTP-binding protein LepAand to LK1236.1 from Caenorhabditis elegans 0.001790.09039&radic&radic&radic S000006131SRP72YPL210CverifiedCore component of the signal recognition particle (SRP)ribonucleoprotein (RNP) complex that functionsin targeting nascent secretory proteins to theendoplasmic reticulum (ER) membrane 0.001790.09039&radic&radic&radic S000000731VAB2YEL005CverifiedProtein with a potential role in vacuolar function, assuggested by its ability to bind Vac8p; greenfluorescent protein (GFP)-fusion proteinlocalizes to the cytoplasm in a punctatepattern 0.001790.09039&radic&radic S000006256NHP6AYPR052CverifiedHigh-mobility group non-histone chromatin protein, functionallyredundant with Nhp6Bp; homologous to mammalianhigh mobility group proteins 1 and 2; acts torecruit transcription factor Rcs1p to certainpromoters 0.001780.08972&radic S000006346YPR142CDubiousHypothetical protein; open reading frame overlaps 5' end of essential RRP15 gene required for ribosomal RNA processing 0.001790.08972&radic&radic S000002456YDR049WUncharacterizedZinc finger protein; putative transcription factor that may interact with proteins involved in histone acetylation or deacetylation; may be involved in altering acetylation on histone lysines 0.001790.08972&radic&radic S000005932TAF3YPL011CverifiedTFIID subunit (47 kDa), involved in promoter binding and RNApolymerase II transcription initiation 0.001790.08972&radic&radic&radic S000000416NGR1YBR212WverifiedRNA binding protein that negatively regulates growth rate;interacts with the 3' UTR of the mitochondrialporin (POR1) mRNA and enhances its degradation;overexpression impairs mitochondrial function;expressed in stationary phase 0.001780.08972&radic&radic&radic S000005635INP53YOR109WverifiedPhosphatidylinositol 4,5-bisphosphate 5-phosphatase,synaptojanin-like protein with an N-terminalSac1 domain, plays a role in a TGN (trans Golginetwork)-to-early endosome pathway;hyperosmotic stress causes translocation toactin patches 0.001790.08972&radic&radic&radic S000005415PSH1YOL054WuncharacterizedNuclear protein, putative RNA polymerase II elongation factor;isolated as Pob3p/Spt16p-binding protein 0.001780.08972&radic&radic&radic&radic S000001597APN1YKL114CverifiedMajor apurinic/apyrimidinic endonuclease, 3'-repair diesteraseinvolved in repair of DNA damage by oxidationand alkylating agents; also functions as a3'-5' exonuclease to repair7,8-dihydro-8-oxodeoxyguanosine 0.001780.08972&radic&radic&radic S000004801GCV2YMR189WverifiedP subunit of the mitochondrial glycine decarboxylase complex,required for the catabolism of glycine to5,10-methylene-THF; expression is regulated bylevels of levels of 5,10-methylene-THF in thecytoplasm 0.001790.08972&radic&radic&radic S000002477FMP16YDR070CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.001780.08972&radic&radic&radic S000002578HSP42YDR171WverifiedSmall cytosolic stress-induced chaperone that formsbarrel-shaped oligomers and suppresses theaggregation of non-native proteins; oligomerdissociation is not required for function;involved in cytoskeleton reorganization afterheat shock 0.001780.08972&radic&radic S000004499YML034C-ADubiousHypothetical protein 0.001790.08972&radic&radic S000002313MSH5YDL154WverifiedProtein of the MutS family, forms a dimer with Msh4p thatfacilitates crossovers between homologs duringmeiosis; msh5-Y823H mutation confers toleranceto DNA alkylating agents; homologs present inC. elegans and humans 0.001780.08972 S000000517GBP2YCL011CverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toHrb1p and Npl3p; also binds single-strandedtelomeric repeat sequence in vitro 0.001780.08972&radic&radic&radic S000003418TFG1YGR186WverifiedTFIIF (Transcription Factor II) largest subunit; involved inboth transcription initiation and elongation ofRNA polymerase II; homologous to human RAP74 0.001780.08972&radic&radic&radic&radic S000006257YPR053CDubiousHypothetical protein 0.001780.08972 S000002801SHE9YDR393WverifiedMitochondrial inner membrane protein required for normalmitochondrial morphology, may be involved infission of the inner membrane; forms ahomo-oligomeric complex 0.001770.08936&radic&radic&radic S000001776BET3YKR068CverifiedHydrophilic protein that acts in conjunction with SNAREproteins in targeting and fusion of ER to Golgitransport vesicles; component of the TRAPP(transport protein particle) complex 0.001770.08936&radic&radic S000003751YJL215CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data 0.001780.08936&radic&radic S000000001TFC3YAL001CverifiedLargest of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauB domain of TFIIIC that binds DNA at theBoxB promoter sites of tRNA and similar genes;cooperates with Tfc6p in DNA binding 0.001780.08936&radic&radic&radic S000003142BUD13YGL174WverifiedSubunit of the RES complex, which is required for nuclearpre-mRNA retention and splicing; involved inbud-site selection; diploid mutants display aunipolar budding pattern instead of thewild-type bipolar pattern 0.001760.08826&radic&radic S000000482DPB3YBR278WverifiedThird-largest subunit of DNA polymerase II (DNA polymeraseepsilon), required to maintain fidelity ofchromosomal replication and also forinheritance of telomeric silencing; mRNAabundance peaks at the G1/S boundary of thecell cycle 0.001760.08826&radic&radic S000002601YDR193WDubiousHypothetical protein 0.001760.08826&radic&radic&radic&radic&radic S000001198YSP1YHR155WuncharacterizedMitochondrial protein with a potential role in promotingmitochondrial fragmentation during programmedcell death in response to high levels ofalpha-factor mating pheromone or the drugamiodarone 0.001760.08826&radic&radic&radic S000000170AAR2YBL074CverifiedComponent of the U5 snRNP, required for splicing of U3precursors; originally described as a splicingfactor specifically required for splicingpre-mRNA of the MATa1 cistron 0.001760.08826&radic S000000574VBA3YCL069WverifiedPermease of basic amino acids in the vacuolar membrane 0.001760.08826&radic&radic&radic S000002812RPB7YDR404CverifiedRNA polymerase II subunit B16; forms two subunit dissociablecomplex with Rpb4p 0.001760.08826&radic&radic S000004665RNA14YMR061WverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; bridges interaction between Rna15p andHrp1p in the CF I complex 0.001750.08826&radic S000002620TCP1YDR212WverifiedAlpha subunit of chaperonin-containing T-complex, whichmediates protein folding in the cytosol;involved in maintenance of actin cytoskeleton;homolog to Drosophila melanogaster and mousetailless complex polypeptide 0.001760.08826&radic&radic&radic S000000667IMG2YCR071CverifiedMitochondrial ribosomal protein of the small subunit 0.001760.08826 S000006376YPR172WUncharacterizedProtein of unknown function, transcriptionally activated by Yrm1p along with genes involved in multidrug resistance 0.001760.08826&radic&radic&radic&radic S000001656SNU114YKL173WverifiedGTPase component of U5 snRNP involved in mRNA splicing viaspliceosome; binds directly to U5 snRNA;proposed to be involved in conformationalchanges of the spliceosome; similarity toribosomal translocation factor EF-2 0.001760.08826&radic&radic S000003857YJR096WUncharacterizedPutative xylose and arabinose reductase 0.001750.08826&radic&radic S000001051YHR009CUncharacterizedPutative protein of unknown function; not an essential gene 0.001750.08826&radic&radic S000002436YDR029WDubiousHypothetical protein 0.001760.08826&radic S000001196SPO16YHR153CverifiedProtein of unknown function, required for spore formation 0.001760.08826&radic&radic&radic&radic S000001098RSC30YHR056CverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; non-essential gene required forregulation of ribosomal protein genes and thecell wall/stress response; highly similar toRsc3p; null mutants are osmosensitive 0.001770.08826 S000000944MAG1YER142Cverified3-methyl-adenine DNA glycosylase involved in protecting DNAagainst alkylating agents; initiates baseexcision repair by removing damaged bases tocreate abasic sites that are subsequentlyrepaired 0.001760.08826&radic S000003216BRR6YGL247WverifiedEssential nuclear envelope integral membrane protein requiredfor nuclear transport; depletion altersnucleoporin distribution and nuclear envelopemorphology, suggesting a role in the spatialorganization of nuclear pores 0.001760.08826&radic S000004770YMR160WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene 0.001750.08774&radic&radic S000000746YEL020CUncharacterizedHypothetical protein with low sequence identity to Pdc1p 0.001750.08774&radic&radic&radic S000003330ESP1YGR098CverifiedSeparase with cysteine protease activity (related to caspases)that promotes sister chromatid separation bymediating dissociation of the cohesin Scc1pfrom chromatin; inhibited by Pds1p 0.001750.08774&radic&radic S000002471RPS13YDR064WverifiedProtein component of the small (40S) ribosomal subunit; hassimilarity to E. coli S15 and rat S13 ribosomalproteins 0.001750.08774&radic&radic S000003098CEG1YGL130WverifiedAlpha (guanylyltransferase) subunit of the mRNA capping enzyme,a heterodimer (the other subunit is CET1, anRNA 5'-triphophatase) involved in adding the 5'cap to mRNA; the mammalian enzyme is a singlebifunctional polypeptide 0.001750.08774&radic&radic S000001317YIL055CUncharacterizedHypothetical protein 0.001740.08774 S000000551YCL046WDubiousHypothetical protein 0.001750.08774&radic&radic S000006267YPR063CUncharacterizedHypothetical protein 0.001740.0875&radic S000000739VAC8YEL013WverifiedPhosphorylated vacuolar membrane protein that interacts withAtg13p, required for the cytoplasm-to-vacuoletargeting (Cvt) pathway; interacts with Nvj1pto form nucleus-vacuole junctions 0.001740.0875&radic&radic S000000732YEA6YEL006WverifiedPutative mitochondrial NAD+ transporter, member of themitochondrial carrier subfamily (see alsoYIA6); has putative human ortholog 0.001740.0875&radic&radic&radic S000005775APC5YOR249CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition 0.001740.0875&radic&radic S000003781YJR020WDubiousHypothetical protein 0.001740.08731&radic S000000663SED4YCR067CverifiedIntegral endoplasmic reticulum membrane protein, functions as apositive regulator of Sar1p probably throughinhibition of GTPase activation by Sec23p;binds Sec16p, participates in vesicleformation, similar to Sec12p 0.001730.08716&radic S000005989YPL068CUncharacterizedHypothetical protein 0.001730.08716&radic&radic&radic&radic S000005129MRPL19YNL185CverifiedMitochondrial ribosomal protein of the large subunit 0.001730.08716&radic&radic&radic&radic S000003834OPI3YJR073CverifiedPhospholipid methyltransferase(methylene-fatty-acyl-phospholipid synthase),catalyzes the last two steps inphosphatidylcholine biosynthesis &radic0.001730.08716 S000003010YGL042CDubiousDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 0.001730.08716&radic S000003666URA2YJL130CverifiedBifunctional carbamoylphosphate synthetase (CPSase)-aspartatetranscarbamylase (ATCase), catalyzes the firsttwo enzymatic steps in the de novo biosynthesisof pyrimidines; both activities are subject tofeedback inhibition by UTP 0.001720.0863&radic&radic&radic S000005384YOL024WUncharacterizedHypothetical protein 0.001720.0863&radic S000003715PFD1YJL179WverifiedSubunit of heterohexameric prefoldin, which binds cytosolicchaperonin and transfers target proteins to it;involved in the biogenesis of actin and ofalpha- and gamma-tubulin 0.001720.0863&radic&radic S000003778ESS1YJR017CverifiedPeptidylprolyl-cis/trans-isomerase (PPIase) specific forphosphorylated serine and threonine residuesN-terminal to proline; regulatesphosphorylation of the RNA polymerase II largesubunit (Rpo21p) C-terminal domain 0.001720.0863&radic&radic S000005531DNL4YOR005CverifiedDNA ligase required for nonhomologous end-joining (NHEJ), formsstable heterodimer with required cofactorLif1p, catalyzes DNA ligation as part of acomplex with Lif1p and Nej1p; involved inmeiosis, not essential for vegetative growth 0.001720.0863&radic&radic S000004621STB4YMR019WverifiedProtein that binds Sin3p in a two-hybrid assay; contains aZn(II)2Cys6 zinc finger domain characteristicof DNA-binding proteins 0.001720.0863&radic&radic&radic&radic S000005291LRO1YNR008WverifiedAcyltransferase that catalyzes diacylglycerol esterification;one of several acyltransferases that contributeto triglyceride synthesis; putative homolog ofhuman lecithin cholesterol acyltransferase 0.001720.0863&radic S000002294RDI1YDL135CverifiedRho GDP dissociation inhibitor involved in the localization andregulation of Cdc42p 0.001720.0863&radic&radic S000005999ATP4YPL078CverifiedSubunit b of the stator stalk of mitochondrial F1F0 ATPsynthase, which is a large, evolutionarilyconserved enzyme complex required for ATPsynthesis 0.001730.0863&radic&radic S000004807RPL36AYMR194WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl36Bpand has similarity to rat L36 ribosomalprotein; binds to 5.8 S rRNA 0.001730.0863&radic&radic&radic S000000919RPL23BYER117WverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl23Ap and has similarity to E.coli L14 and rat L23 ribosomal proteins 0.001730.0863&radic S000000101PDR3YBL005WverifiedTranscriptional activator of the pleiotropic drug resistancenetwork, regulates expression of ATP-bindingcassette (ABC) transporters through binding tocis-acting sites known as PDREs (PDR responsiveelements) 0.001730.0863&radic&radic S000002468YDR061WUncharacterizedMitochondrial protein, member of the ATP-binding cassette (ABC) transporter family; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance 0.001720.0863&radic&radic&radic&radic&radic S000003804POL32YJR043CverifiedThird subunit of DNA polymerase delta, involved in chromosomalDNA replication; required for error-prone DNAsynthesis in the presence of DNA damage andprocessivity; interacts with Hys2p, PCNA(Pol30p), and Pol1p 0.001720.0863&radic&radic S000001634YKL151CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.001730.0863&radic&radic S000004900DSS1YMR287CverifiedRNase, component of the mitochondrial degradosome along withthe ATP-dependent RNA helicase Suv3p; thedegradosome associates with the ribosome andmediates turnover of aberrant or unprocessedRNAs 0.001730.0863&radic&radic S000000755BUD16YEL029CverifiedProtein involved in bud-site selection and telomeremaintenance; diploid mutants display a randombudding pattern instead of the wild-typebipolar pattern; has similarity to pyridoxalkinases 0.001730.0863&radic&radic&radic S000003750HXT8YJL214WverifiedProtein of unknown function with similarity to hexosetransporter family members, expression isinduced by low levels of glucose and repressedby high levels of glucose 0.001720.0863&radic&radic&radic S000001167YHR125WDubiousHypothetical protein 0.001720.0863&radic S000005685SME1YOR159CverifiedCore Sm protein Sm E; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm E 0.001730.0863&radic S000002652PEX5YDR244WverifiedPeroxisomal membrane signal receptor for C-terminal tripeptidesignal sequence (PTS1) of peroxisomal matrixproteins, required for peroxisomal matrixprotein import, tetratricopeptide repeatprotein, also involved in PTS1-independentimport 0.001730.0863&radic&radic&radic&radic S000001601YKL118WDubiousDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 0.001720.08532&radic&radic&radic S000001195SPO12YHR152WverifiedNucleolar protein of unknown function, positive regulator ofexit from mitosis; involved in regulating therelease of Cdc14p from the nucleolus in earlyanaphase; proposed to play similar role inmeiosis 0.001720.08532&radic&radic&radic&radic S000004270YLR280CDubiousHypothetical protein 0.001710.08532&radic&radic&radic&radic S000005168SQS1YNL224CverifiedProtein of unknown function; overexpression antagonizes thesuppression of splicing defects by spp382mutants; green fluorescent protein (GFP)-fusionprotein localizes to both the cytoplasm and thenucleus 0.001710.08532&radic&radic&radic&radic&radic S000000102LDB7YBL006CverifiedComponent of the RSC chromatin remodeling complex; interactswith Rsc3p, Rsc30p, Npl6p, and Htl1p to form amodule important for a broad range of RSCfunctions 0.001710.08532&radic&radic S000000974BRR2YER172CverifiedRNA-dependent ATPase RNA helicase involved in the facilitationand disruption of snRNA interactions, requiredfor disruption of U4/U6 base-pairing in nativesnRNPs to activate the spliceosome forcatalysis <