Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0016078 "tRNA catabolism"

GO branch: BP
Number of currently annotated genes: 4

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest Ho:S000001341Ho:S000002334Giaever:5-fluorouracil_76.9uMHo:S000005475Harbison:MSS11_BUT14TAP:36TAP:129Huh:nucleusTAP:123Harbison:FHL1_H2O2Hi S000005243TRF5YNL299WverifiedPoly (A) polymerase involved in nuclear RNA quality controlbased on: homology with Trf4p, geneticinteractions with TRF4 mutants, physicalinteraction with Mtr4p (TRAMP subunit), and bydirect assay; disputed role as a sigma DNApolymerase 0.162490.88636 S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p &radic0.076430.69042&radic&radic&radic&radic S000002334AIR2YDL175CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p; may regulatemethylation of Npl3p, which modulates Npl3pfunction in mRNA processing and export; hassimilarity to Air1p &radic0.063780.6533&radic&radic&radic S000006111NAB3YPL190CverifiedSingle stranded DNA binding protein; acidic ribonucleoprotein;required for termination of non-poly(A)transcripts and efficient splicing; interactswith Nrd1p 0.025020.48658&radic&radic S000003163GCN1YGL195WverifiedPositive regulator of the Gcn2p kinase activity, forms acomplex with Gcn20p; proposed to stimulateGcn2p activation by an uncharged tRNA 0.012110.35591 S000002495SLU7YDR088CverifiedRNA splicing factor, required for ATP-independent portion of2nd catalytic step of spliceosomal RNAsplicing; interacts with Prp18p; contains zincknuckle domain 0.005160.22805&radic S000005199GIS2YNL255CverifiedProtein with seven cysteine-rich CCHC zinc-finger motifs,similar to human CNBP, proposed to be involvedin the RAS/cAMP signaling pathway 0.00510.22493 S000004106MSL5YLR116WverifiedComponent of the commitment complex, which defines the firststep in the splicing pathway; essential proteinthat interacts with Mud2p and Prp40p, forming abridge between the intron ends; also involvedin nuclear retention of pre-mRNA 0.004990.22105&radic S000004390SKI2YLR398CverifiedPutative RNA helicase, involved in exosome mediated 3' to 5'mRNA degradation and translation inhibition ofnon-poly(A) mRNAs; forms complex with Ski3p andSki8p; required for repressing propagation ofdsRNA viruses 0.004280.19113 S000001710PAP1YKR002WverifiedPoly(A) polymerase, one of three factors required for mRNA3'-end polyadenylation, forms multiproteincomplex with polyadenylation factor I (PF I),also required for mRNA nuclear export; may alsopolyadenylate rRNAs 0.003150.14586&radic S000000939YER137CUncharacterizedHypothetical protein 0.002820.13228 S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif 0.002730.12889&radic S000006311YTH1YPR107CverifiedEssential RNA-binding component of cleavage and polyadenylationfactor, contains five zinc fingers; requiredfor pre-mRNA 3'-end processing andpolyadenylation 0.002640.12478&radic S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.001230.06046 S000003506TAF1YGR274CverifiedTFIID subunit (145 kDa), involved in RNA polymerase IItranscription initiation, has histoneacetyltransferase activity, involved inpromoter binding and G1/S progression 0.001090.05245&radic&radic S000005475PAP2YOL115WverifiedCatalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), anuclear poly (A) polymerase complex involved inRNA quality control; catalyzes polyadenylationof unmodified tRNAs, and snoRNA and rRNAprecursors; disputed role as a DNA polymerase &radic0.000910.04127&radic&radic&radic S000003586MTR4YJL050WverifiedDead-box family ATP dependent helicase required for mRNA exportfrom the nucleus; co-factor of the exosomecomplex, required for 3' end formation of 5.8SrRNA &radic0.000890.04054&radic&radic&radic&radic&radic S000001111RRP4YHR069CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp43p and Dis3p 0.000640.03066&radic S000005206POL2YNL262WverifiedCatalytic subunit of DNA polymerase epsilon, one of the majorchromosomal DNA replication polymerasescharacterized by processivity and proofreadingexonuclease activity; also involved in DNAsynthesis during DNA repair 0.000560.02682&radic S000001377NUP159YIL115CverifiedSubunit of the nuclear pore complex that is found exclusivelyon the cytoplasmic side, forms a subcomplexwith Nup82p and Nsp1p, required for mRNA export 0.000480.02184 S000005366TOP1YOL006CverifiedTopoisomerase I, nuclear enzyme that relieves torsional strainin DNA by cleaving and re-sealing thephosphodiester backbone; relaxes bothpositively and negatively supercoiled DNA;functions in replication, transcription, andrecombination 0.000460.01976&radic S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.000450.01935&radic&radic&radic S000004577ZDS2YML109WverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; paralogof Zds1p 0.000350.01235 S000000631RRP43YCR035CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp4p and Dis3p; required for efficientmaturation of 5.8S, 18S and 25S rRNA 0.00030.00843&radic&radic S000002360TRM8YDL201WverifiedSubunit of a tRNA methyltransferase complex composed of Trm8pand Trm82p that catalyzes 7-methylguanosinemodification of tRNA 0.000290.00834&radic S000000238HMT1YBR034CverifiedNuclear SAM-dependent mono- and asymmetric argininedimethylating methyltransferase that modifieshnRNPs, including Npl3p and Hrp1p, thusfacilitating nuclear export of these proteins;required for viability of npl3 mutants 0.000270.00653&radic S000002789YRA1YDR381WverifiedNuclear protein that binds to RNA and to Mex67p, required forexport of poly(A)+ mRNA from the nucleus;member of the REF (RNA and export factorbinding proteins) family; another familymember, Yra2p, can substitute for Yra1pfunction 0.000230.00392&radic S000005730DED1YOR204WverifiedATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, requiredfor translation initiation of all yeast mRNAs;mutations in human DEAD-box DBY are a frequentcause of male infertility 0.000230.00392 S000006379DPB2YPR175WverifiedSecond largest subunit of DNA polymerase II (DNA polymeraseepsilon), required for normal yeast chromosomalreplication; expression peaks at the G1/S phaseboundary; potential Cdc28p substrate 0.000220.00328&radic S000004284GSP1YLR293CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; regulated by Prp20p,Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p andKap95p; yeast Gsp2p homolog 0.000210.00294&radic S000005187SLA2YNL243WverifiedTransmembrane actin-binding protein involved in membranecytoskeleton assembly and cell polarization;adaptor protein that links actin to clathrinand endocytosis; present in the actin corticalpatch of the emerging bud tip; dimer in vivo 0.00020.00284 S000005056DBP2YNL112WverifiedEssential ATP-dependent RNA helicase of the DEAD-box proteinfamily, involved in nonsense-mediated mRNAdecay and rRNA processing 0.00020.00278&radic S000004409VPS36YLR417WverifiedComponent of the ESCRT-II complex; contains the GLUE (GRAM LikeUbiquitin binding in EAP45) domain which isinvolved in interactions with ESCRT-I andubiquitin-dependent sorting of proteins intothe endosome 0.00020.00278&radic S000004349RSC2YLR357WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; involvedin telomere maintenance 0.00020.00278&radic S000005028END3YNL084CverifiedEH domain-containing protein involved in endocytosis, actincytoskeletal organization and cell wallmorphogenesis; forms a complex with Sla1p andPan1p 0.00020.00271&radic&radic S000000120NCL1YBL024WverifiedS-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase,methylates cytosine to m5C at several positionsin tRNAs and intron-containing pre-tRNAs;similar to Nop2p and human proliferationassociated nucleolar protein p120 0.00020.00271&radic S000000682CSM1YCR086WverifiedNucleolar protein that forms a complex with Lrs4p which bindsMam1p at kinetochores during meiosis I tomediate accurate chromosome segregation, may beinvolved in premeiotic DNA replication;possible role in telomere maintenance 0.00020.00271&radic&radic S000004886ZDS1YMR273CverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; has arole in localization of Bcy1p, a regulatorysubunit of protein kinase A; implicated in mRNAnuclear export 0.00020.00271 S000004172SWI6YLR182WverifiedTranscription cofactor, forms complexes with DNA-bindingproteins Swi4p and Mbp1p to regulatetranscription at the G1/S transition; involvedin meiotic gene expression; localizationregulated by phosphorylation; potential Cdc28psubstrate 0.00020.00271&radic&radic S000002160NHP10YDL002CverifiedProtein related to mammalian high mobility group proteins;likely component of the INO80 complex, which isan ATP-dependent chromatin-remodeling complex 0.00020.00271&radic S000003288RSC1YGR056WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; containstwo essential bromodomains, a bromo-adjacenthomology (BAH) domain, and an AT hook 0.000190.00261 S000004181PEX13YLR191WverifiedIntegral peroxisomal membrane receptor for the PTS1 peroxisomalmatrix protein signal recognition factor Pex5p,required for the translocation of peroxisomalmatrix proteins, also interacts with Pex7p andPex14p, contains a C-terminal SH3 domain 0.000190.00257 S000002536SAC6YDR129CverifiedFimbrin, actin-bundling protein; cooperates with Scp1p(calponin/transgelin) in the organization andmaintenance of the actin cytoskeleton 0.000190.00257 S000004415ATG17YLR423CverifiedProtein that interacts with and is required for activation ofApg1p protein kinase; involved in autophagy butnot in the Cvt (cytoplasm to vacuole targeting)pathway 0.000190.00253&radic S000005372HTZ1YOL012CverifiedHistone variant H2AZ, exchanged for histone H2A in nucleosomesby the SWR1 complex; involved intranscriptional regulation through preventionof the spread of silent heterochromatin 0.000190.00253&radic S000001790NUP133YKR082WverifiedSubunit of the Nup84p subcomplex of the nuclear pore complex(NPC), localizes to both sides of the NPC,required to establish a normalnucleocytoplasmic concentration gradient of theGTPase Gsp1p 0.000190.00253 S000006005BRO1YPL084WverifiedCytoplasmic class E vacuolar protein sorting (VPS) factor thatcoordinates deubiquitination in themultivesicular body (MVB) pathway by recruitingDoa4p to endosomes 0.000190.00242 S000004470SPT5YML010WverifiedProtein that forms a complex with Spt4p and mediates bothactivation and inhibition of transcriptionelongation; Spt4p-Spt5p complex also plays arole in pre-mRNA processing 0.000190.00242&radic S000004984BDP1YNL039WverifiedEssential subunit of RNA polymerase III transcription factor(TFIIIB), which is involved in transcription ofgenes encoding tRNAs, 5S rRNA, U6 snRNA, andother small RNAs 0.000180.00241&radic S000005356YNR073CUncharacterizedHypothetical protein 0.000180.00241 S000002742SWR1YDR334WverifiedSwi2/Snf2-related ATPase that is the structural component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.000180.00233&radic&radic&radic S000006395QCR2YPR191WverifiedSubunit 2 of the ubiquinol cytochrome-c reductase complex,which is a component of the mitochondrial innermembrane electron transport chain;transcription is regulated by Hap1p,Hap2p/Hap3p, and heme 0.000180.00233 S000000039CDC24YAL041WverifiedGuanine nucleotide exchange factor (GEF or GDP-release factor)for Cdc42p; required for polarity establishmentand maintenance, and mutants have morphologicaldefects in bud formation and shmooing 0.000180.00226&radic S000001387KGD1YIL125WverifiedComponent of the mitochondrial alpha-ketoglutaratedehydrogenase complex, which catalyzes a keystep in the tricarboxylic acid (TCA) cycle, theoxidative decarboxylation ofalpha-ketoglutarate to form succinyl-CoA 0.000170.00224 S000002430SES1YDR023WverifiedCytosolic seryl-tRNA synthetase, class II aminoacyl-tRNAsynthetase that aminoacylates tRNA(Ser),displays tRNA-dependent amino acid recognitionwhich enhances discrimination of the serinesubstrate, interacts with peroxin Pex21p 0.000170.00224 S000006067NOP53YPL146CverifiedNucleolar protein; involved in biogenesis of the 60S subunit ofthe ribosome; interacts with rRNA processingfactors Cbf5p and Nop2p; null mutant is viablebut growth is severely impaired 0.000170.0022&radic S000000435SWC5YBR231CuncharacterizedProtein of unknown function, component of the SWR1 complex,which exchanges histone variant H2AZ (Htz1p)for chromatin-bound histone H2A 0.000170.00214&radic S000005143GCR2YNL199CverifiedTranscriptional activator of genes involved in glycolysis;interacts and functions with the DNA-bindingprotein Gcr1p 0.000170.00213 S000001073RRM3YHR031CverifiedDNA helicase involved in rDNA replication and Ty1transposition; relieves replication fork pausesat telomeric regions; structurally andfunctionally related to Pif1p 0.000170.00213&radic S000002847LRS4YDR439WverifiedProtein involved in rDNA silencing; positively chargedcoiled-coil protein with limited similarity tomyosin 0.000170.00213&radic S000004520IMD4YML056CverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.000170.00213 S000003397MRPS35YGR165WverifiedMitochondrial ribosomal protein of the small subunit 0.000160.00212 S000001234CTF8YHR191CverifiedSubunit of a complex with Ctf18p that shares some subunits withReplication Factor C and is required for sisterchromatid cohesion 0.000160.00211 S000004363ROM2YLR371WverifiedGDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutationsare synthetically lethal with mutations inrom1, which also encodes a GEP 0.000160.00211 S000005667ARP8YOR141CverifiedNuclear actin-related protein involved in chromatin remodeling,component of chromatin-remodeling enzymecomplexes 0.000160.00211&radic S000003088PRP43YGL120CverifiedRNA helicase in the DEAH-box family, functions in both RNApolymerase I and polymerase II transcriptmetabolism, involved in release of thelariat-intron from the spliceosome 0.000160.00209 S000006177CLN2YPL256CverifiedG1 cyclin involved in regulation of the cell cycle; activatesCdc28p kinase to promote the G1 to S phasetransition; late G1 specific expression dependson transcription factor complexes, MBF(Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) 0.000160.00209&radic S000003660LSM1YJL124CverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)involved in degradation of cytoplasmic mRNAs 0.000160.00207 S000004029RIC1YLR039CverifiedProtein involved in retrograde transport to the cis-Golginetwork; forms heterodimer with Rgp1p that actsas a GTP exchange factor for Ypt6p; involved intranscription of rRNA and ribosomal proteingenes 0.000160.00207 S000003336SRB5YGR104CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation; involved intelomere maintenance 0.000160.00206&radic S000001756NAP1YKR048CverifiedProtein that interacts with mitotic cyclin Clb2p; required forthe regulation of microtubule dynamics duringmitosis; controls bud morphogenesis; involvedin the transport of H2A and H2B histones to thenucleus 0.000160.00206 S000004732STO1YMR125WverifiedLarge subunit of the nuclear mRNA cap-binding protein complex,interacts with Npl3p to carry nuclear poly(A)+mRNA to cytoplasm; also involved in nuclearmRNA degradation and telomere maintenance;orthologous to mammalian CBP80 0.000160.00206&radic&radic S000002544RGP1YDR137WverifiedSubunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) thatcatalyzes nucleotide exchange on Ypt6p 0.000160.00202 S000003063VPS45YGL095CverifiedProtein of the Sec1p/Munc-18 family, essential for vacuolarprotein sorting; required for the function ofPep12p and the early endosome/late Golgi SNARETlg2p; essential for fusion of Golgi-derivedvesicles with the prevacuolar compartment 0.000160.00202 S000000968DNF1YER166WverifiedAminophospholipid translocase (flippase) that localizesprimarily to the plasma membrane; contributesto endocytosis, protein transport and cellpolarity; type 4 P-type ATPase 0.000160.00202 S000004467ERG6YML008CverifiedDelta(24)-sterol C-methyltransferase, converts zymosterol tofecosterol in the ergosterol biosyntheticpathway by methylating position C-24; localizedto both lipid particles and mitochondrial outermembrane 0.000160.00202 S000005195NRD1YNL251CverifiedRNA-binding protein that interacts with the C-terminal domainof the RNA polymerase II large subunit(Rpo21p), required for transcriptiontermination and 3' end maturation ofnonpolyadenylated RNAs 0.000160.002&radic&radic S000003406CBP4YGR174CverifiedMitochondrial protein required for assembly of ubiquinolcytochrome-c reductase complex (cytochrome bc1complex); interacts with Cbp3p and function ispartially redundant with that of Cbp3p 0.000150.00197&radic S000004138PEP3YLR148WverifiedVacuolar peripheral membrane protein that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, required for vacuolarbiogenesis, forms complex with Pep5p thatmediates protein transport to the vacuole 0.000150.00197 S000004682VPS20YMR077CverifiedMyristoylated subunit of ESCRTIII, the endosomal sortingcomplex required for transport of transmembraneproteins into the multivesicular body pathwayto the lysosomal/vacuolar lumen; cytoplasmicprotein recruited to endosomal membranes 0.000150.00196 S000005738STE4YOR212WverifiedG protein beta subunit, forms a dimer with Ste18p to activatethe mating signaling pathway, forms aheterotrimer with Gpa1p and Ste18p to dampensignaling; may recruit Rho1p to the polarizedgrowth site during mating; contains WD40repeats 0.000150.00193 S000004424IMD3YLR432WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.000140.00189 S000002905ITR1YDR497CverifiedMyo-inositol transporter with strong similarity to the minormyo-inositol transporter Itr2p, member of thesugar transporter superfamily; expression isrepressed by inositol and choline via Opi1p andderepressed via Ino2p and Ino4p 0.000140.00184&radic S000002777XRS2YDR369CverifiedProtein required for DNA repair; component of the Mre11complex, which is involved in double strandbreaks, meiotic recombination, telomeremaintenance, and checkpoint signaling 0.000130.00182&radic&radic S000002767VID21YDR359CverifiedComponent of the NuA4 histone acetyltransferase complex 0.000130.00182 S000000286UBC4YBR082CverifiedUbiquitin-conjugating enzyme that mediates degradation ofshort-lived and abnormal proteins; interactswith E3-CaM in ubiquitinating calmodulin;interacts with many SCF ubiquitin proteinligases; component of the cellular stressresponse 0.000140.00182&radic S000004329VRP1YLR337CverifiedProline-rich actin-associated protein involved in cytoskeletalorganization and cytokinesis; related tomammalian Wiskott-Aldrich syndrome protein(WASP)-interacting protein (WIP) 0.000130.00178 S000001129YHR087WVerifiedProtein involved in RNA metabolism, one of two yeast homologs (with Sdo1p/Ylr022cp) of the human protein SBDS responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome, also conserved in Archaea 0.000130.00176&radic S000003054MAD1YGL086WverifiedCoiled-coil protein involved in the spindle-assemblycheckpoint; phosphorylated by Mps1p uponcheckpoint activation which leads to inhibitionof the activity of the anaphase promotingcomplex; forms a complex with Mad2p 0.000130.00176&radic S000005923SNF8YPL002CverifiedComponent of the ESCRT-II complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; appears to be functionallyrelated to SNF7; involved in glucosederepression 0.000130.00174 S000005552BUB3YOR026WverifiedKinetochore checkpoint WD40 repeat protein that localizes tokinetochores during prophase and metaphase,delays anaphase in the presence of unattachedkinetochores; forms complexes with Mad1p-Bub1pand with Cdc20p, binds Mad2p and Mad3p 0.000130.00174&radic S000002545HPR1YDR138WverifiedSubunit of THO/TREX complexes that couple transcriptionelongation with mitotic recombination and withmRNA metabolism and export, subunit of an RNAPol II complex; regulates lifespan; involved intelomere maintenance; similar to Top1p 0.000130.00174&radic S000005051YAF9YNL107WverifiedSubunit of both the NuA4 histone H4 acetyltransferase complexand the SWR1 complex, may function toantagonize silencing near telomeres; interactsdirectly with Swc4p, has homology to humanleukemogenic protein AF9, contains a YEATSdomain 0.000130.00174&radic S000006305SNT309YPR101WverifiedComponent of NineTeen complex (NTC) containing Prp19p involvedin mRNA splicing, interacts physically andgenetically with Prp19p 0.000120.00173&radic S000004075ARP6YLR085CverifiedActin-related protein that binds nucleosomes; a component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.000120.00173&radic S000000913SWI4YER111CverifiedDNA binding component of the SBF complex (Swi4p-Swi6p), atranscriptional activator that in concert withMBF (Mbp1-Swi6p) regulates late G1-specifictranscription of targets including cyclins andgenes required for DNA synthesis and repair 0.000120.00173&radic S000004949HRB1YNL004WverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toGbp2p and Npl3p 0.000120.00173&radic S000001927SMC2YFR031CverifiedComponent of the condensin complex, essential SMC chromosomalATPase family member that forms a complex withSmc4p to form the active ATPase; Smc2p/Smc4pcomplex binds DNA, possibly in the cleft formedby the coiled-coil of the folded dimer 0.000120.00173&radic S000005480RPL18AYOL120CverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl18Bp and has similarity to ratL18 ribosomal protein; intron of RPL18Apre-mRNA forms stem-loop structures that are atarget for Rnt1p cleavage leading todegradation 0.000120.00173 S000000770IES6YEL044WverifiedProtein that associates with the INO80 chromatin remodelingcomplex under low-salt conditions 0.000120.00173&radic S000000301VPS15YBR097WverifiedMyristoylated serine/threonine protein kinase involved invacuolar protein sorting; functions as amembrane-associated complex with Vps34p; activeform recruits Vps34p to the Golgi membrane;interacts with the GDP-bound form of Gpa1p 0.000120.00173 S000004187SIK1YLR197WverifiedEssential evolutionarily-conserved nucleolar protein componentof the box C/D snoRNP complexes that direct2'-O-methylation of pre-rRNA during itsmaturation; overexpression causes spindleorientation defects 0.000120.00171 S000005169CNM67YNL225CverifiedComponent of the spindle pole body outer plaque; required forspindle orientation and mitotic nuclearmigration 0.000120.00171 S000000322TEF2YBR118WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF1; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.000120.00171 S000000122LSM2YBL026WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.000120.0017&radic S000001596RAD27YKL113Cverified5' to 3' exonuclease, 5' flap endonuclease, required forOkazaki fragment processing and maturation aswell as for long-patch base-excision repair;member of the S. pombe RAD2/FEN1 family 0.000120.0017&radic S000000673PAT1YCR077CverifiedTopoisomerase II-associated deadenylation-dependentmRNA-decapping factor; also required forfaithful chromosome transmission, maintenanceof rDNA locus stability, and protection of mRNA3'-UTRs from trimming; functionally linked toPab1p 0.000120.0017 S000003863VPS25YJR102CverifiedComponent of the ESCRT-II complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome 0.000120.00169 S000005831ISW2YOR304WverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPasecomponent that, with Itc1p, forms a complexrequired for repression of a-specific genes,INO1, and early meiotic genes during mitoticgrowth 0.000120.00169&radic&radic S000004683CTF18YMR078CverifiedSubunit of a complex with Ctf8p that shares some subunits withReplication Factor C and is required for sisterchromatid cohesion; may have overlappingfunctions with Rad24p in the DNA damagereplication checkpoint 0.000120.00169&radic S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.000120.00169&radic S000002555KGD2YDR148CverifiedDihydrolipoyl transsuccinylase, a component of themitochondrial alpha-ketoglutarate dehydrogenasecomplex, which catalyzes a step in thetricarboxylic acid (TCA) cycle, the oxidativedecarboxylation of alpha-ketoglutarate tosuccinyl-CoA 0.000120.00167 S000004362ARC18YLR370CverifiedSubunit of the ARP2/3 complex, which is required for themotility and integrity of cortical actinpatches 0.000120.00167 S000003062PAN2YGL094CverifiedEssential subunit of the Pan2p-Pan3p poly(A)-ribonucleasecomplex, which acts to control poly(A) taillength and regulate the stoichiometry andactivity of postreplication repair complexes 0.000120.00166 S000004045SPT8YLR055CverifiedSubunit of the SAGA transcriptional regulatory complex but notpresent in SAGA-like complex SLIK/SALSA,required for SAGA-mediated inhibition at somepromoters 0.000120.00166&radic S000004920UBP15YMR304WverifiedUbiquitin-specific protease that may play a role in ubiquitinprecursor processing 0.000120.00166&radic S000003095SOH1YGL127CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; involved in telomeremaintenance; conserved with other metazoanMED31 subunits 0.000120.00166&radic S000004252YPT6YLR262CverifiedGTPase, Ras-like GTP binding protein involved in the secretorypathway, required for fusion ofendosome-derived vesicles with the late Golgi,maturation of the vacuolar carboxypeptidase Y;has similarity to the human GTPase, Rab6 0.000120.00166 S000004745PSO2YMR137CverifiedRequired for a post-incision step in the repair of DNA singleand double-strand breaks that result frominterstrand crosslinks produced by a variety ofmono- and bi-functional psoralen derivatives;induced by UV-irradiation 0.000120.00166&radic&radic S000001886SMC1YFL008WverifiedSubunit of the multiprotein cohesin complex, essential proteininvolved in chromosome segregation and indouble-strand DNA break repair; SMC chromosomalATPase family member, binds DNA with apreference for DNA with secondary structure 0.000120.00166&radic&radic S000000455MRPS5YBR251WverifiedMitochondrial ribosomal protein of the small subunit 0.000120.00166&radic S000002688RRP45YDR280WverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.000120.00166&radic S000001123LRP1YHR081WverifiedSubstrate-specific nuclear cofactor for exosome activity in theprocessing of stable RNAs; required fortelomere length maintenance; homolog ofmammalian nuclear matrix protein C1D involvedin regulation of DNA repair and recombination 0.000110.00164 S000004597MSC1YML128CverifiedProtein of unknown function; mutant is defective in directingmeiotic recombination events to homologouschromatids; the authentic, non-tagged proteinis detected in highly purified mitochondria inhigh-throughput studies 0.000110.00163 S000004903HAS1YMR290CverifiedATP-dependent RNA helicase; localizes to both the nuclearperiphery and nucleolus; highly enriched innuclear pore complex fractions; constituent of66S pre-ribosomal particles 0.000110.00163 S000005194RAD50YNL250WverifiedSubunit of MRX complex, with Mre11p and Xrs2p, involved inprocessing double-strand DNA breaks invegetative cells, initiation of meiotic DSBs,telomere maintenance, and nonhomologous endjoining 0.000110.00163&radic S000003797HUL4YJR036CverifiedProtein with similarity to hect domain E3 ubiquitin-proteinligases, not essential for viability 0.000110.00163 S000000602RVS161YCR009CverifiedAmphiphysin-like lipid raft protein; subunit of a complex(Rvs161p-Rvs167p) that regulates polarizationof the actin cytoskeleton, endocytosis, cellpolarity, cell fusion and viability followingstarvation or osmotic stress 0.000110.00163 S000002419RPL4BYDR012WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl4Ap and has similarity to E.coli L4 and rat L4 ribosomal proteins 0.000110.00161&radic S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 0.000110.00161&radic S000000967PAB1YER165WverifiedPoly(A) binding protein, part of the 3'-end RNA-processingcomplex, mediates interactions between the 5'cap structure and the 3' mRNA poly(A) tail,involved in control of poly(A) tail length,interacts with translation factor eIF-4G 0.000110.00159 S000002467MAK21YDR060WverifiedConstituent of 66S pre-ribosomal particles, required for large(60S) ribosomal subunit biogenesis; involved innuclear export of pre-ribosomes; required formaintenance of dsRNA virus; homolog of humanCAATT-binding protein 0.000110.00159&radic S000001238NVJ1YHR195WverifiedNuclear envelope protein that interacts with the vacuolarmembrane protein Vac8p to promote formation ofnucleus-vacuole junctions during piecemealmicroautophagy of the nucleus (PMN) 0.000110.00159&radic S000005133SRP1YNL189WverifiedKaryopherin alpha homolog, forms a dimer with karyopherin betaKap95p to mediate import of nuclear proteins,binds the nuclear localization signal of thesubstrate during import; may also play a rolein regulation of protein degradation 0.000110.00157 S000006141RPL1AYPL220WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl1Bpand has similarity to E. coli L1 and rat L10aribosomal proteins; rpl1a rpl1b double nullmutation is lethal 0.000110.00157 S000004918ADH2YMR303CverifiedGlucose-repressible alcohol dehydrogenase II, catalyzes theconversion of ethanol to acetaldehyde; involvedin the production of certain carboxylateesters; regulated by ADR1 0.000110.00157 S000004925PSE1YMR308CverifiedKaryopherin/importin that interacts with the nuclear porecomplex; acts as the nuclear import receptorfor specific proteins, including Pdr1p, Yap1p,Ste12p, and Aft1p 0.000110.00157&radic S000001789RPF2YKR081CverifiedEssential protein involved in the processing of pre-rRNA andthe assembly of the 60S ribosomal subunit;interacts with ribosomal protein L11; localizespredominantly to the nucleolus; constituent of66S pre-ribosomal particles 0.00010.00154&radic S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 0.00010.00154&radic S000005820RRS1YOR294WverifiedEssential protein that binds ribosomal protein L11 and isrequired for nuclear export of the 60Spre-ribosomal subunit during ribosomebiogenesis; mouse homolog shows alteredexpression in Huntington's disease model mice 0.00010.00154 S000006082BEM4YPL161CverifiedProtein involved in establishment of cell polarity and budemergence; interacts with the Rho1p smallGTP-binding protein and with the Rho-typeGTPase Cdc42p; involved in maintenance ofproper telomere length 0.00010.00154&radic S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus 0.00010.00154&radic&radic&radic S000002718SUM1YDR310CverifiedTranscriptional repressor required for mitotic repression ofmiddle sporulation-specific genes; involved intelomere maintenance, regulated by thepachytene checkpoint 0.00010.00154&radic S000001508PAN3YKL025CverifiedEssential subunit of the Pan2p-Pan3p poly(A)-ribonucleasecomplex, which acts to control poly(A) taillength and regulate the stoichiometry andactivity of postreplication repair complexes 0.00010.00152&radic&radic S000000514STP22YCL008CverifiedComponent of the ESCRT-I complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; homologous to the mouse and humanTsg101 tumor susceptibility gene; mutantsexhibit a Class E Vps phenotype 0.00010.00152 S000004476TRM9YML014WverifiedtRNA methyltransferase, catalyzes the esterification ofmodified uridine nucleotides in tRNAs, creating5-methylcarbonylmethyluridine in tRNA(Arg3) and5-methylcarbonylmethyl-2-thiouridine intRNA(Glu); may have a role in stress response 0.00010.00152 S000004494RAD52YML032CverifiedProtein that stimulates strand exchange by facilitating Rad51pbinding to single-stranded DNA; annealscomplementary single-stranded DNA; involved inthe repair of double-strand breaks in DNAduring vegetative growth and meiosis 0.00010.00152&radic S000005606DIA2YOR080WverifiedOrigin-binding F-box protein that forms an SCF ubiquitin ligasecomplex with Skp1p and Cdc53p; plays a role inDNA replication, involved in invasive andpseudohyphal growth 0.00010.00152&radic&radic S000003746PEX2YJL210WverifiedRING-finger peroxin, peroxisomal membrane protein with aC-terminal zinc-binding RING domain, formsputative translocation subcomplex with Pex10pand Pex12p which functions in peroxisomalmatrix protein import 0.00010.00152 S000004312MMS22YLR320WverifiedProtein involved in resistance to ionizing radiation; acts withMms1p in a repair pathway that may be involvedin resolving replication intermediates orpreventing the damage caused by blockedreplication forks 0.00010.00152 S000003719MNN11YJL183WverifiedSubunit of a Golgi mannosyltransferase complex that alsocontains Anp1p, Mnn9p, Mnn10p, and Hoc1p, andmediates elongation of the polysaccharidemannan backbone; has homology to Mnn10p 0.00010.0015 S000000744EAF5YEL018WuncharacterizedEsa1p-associated factor, subunit of the NuA4 acetyltransferasecomplex 0.00010.0015&radic&radic S000003121PEX14YGL153WverifiedPeroxisomal membrane protein that is a central component of theperoxisomal protein import machinery, interactswith PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomalmatrix protein signal recognition factors andmembrane receptor Pex13p 9e-050.00148 S000000818BIM1YER016WverifiedMicrotubule-binding protein that together with Kar9p makes upthe cortical microtubule capture site anddelays the exit from mitosis when the spindleis oriented abnormally 9e-050.00148 S000004844PEP5YMR231WverifiedPeripheral vacuolar membrane protein required for proteintrafficking and vacuole biogenesis; formscomplex with Pep3p that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, also interacts with Pep7p 9e-050.00148 S000004498AMD1YML035CverifiedAMP deaminase, tetrameric enzyme that catalyzes the deaminationof AMP to form IMP and ammonia; may be involvedin regulation of intracellular adeninenucleotide pools 4e-050.00114 S000003994THI73YLR004CuncharacterizedPutative plasma membrane permease proposed to be involved incarboxylic acid uptake and repressed bythiamine; substrate of Dbf2p/Mob1p kinase;transcription is altered if mitochondrialdysfunction occurs 4e-050.00114 S000005942ECM23YPL021WverifiedNon-essential protein of unconfirmed function; affects pre-rRNAprocessing, may act as a negative regulator ofthe transcription of genes involved inpseudohyphal growth; homologous to Srd1p 4e-050.00113 S000003074MLC1YGL106WverifiedEssential light chain for myosin Myo2p; may stabilize Myo2p bybinding to the neck region; may interact withMyo1p, Iqg1p, and Myo2p to coordinate formationand contraction of the actomyosin ring withtargeted membrane deposition 4e-050.00113 S000000333OPY1YBR129CverifiedProtein of unknown function, overproduction blocks cell cyclearrest in the presence of mating pheromone; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 4e-050.00111 S000000664ATG15YCR068WverifiedLipase, required for intravacuolar lysis of autophagic bodies;located in the endoplasmic reticulum membraneand targeted to intravacuolar vesicles duringautophagy via the multivesicular body (MVB)pathway 4e-050.00111&radic S000004802SGS1YMR190CverifiedNucleolar DNA helicase of the RecQ family involved inmaintenance of genome integrity, regulateschromosome synapsis and meiotic crossing over;has similarity to human BLM and WRN helicasesimplicated in Bloom and Werner syndromes 4e-050.00111&radic S000004848RNA1YMR235CverifiedGTPase activating protein (GAP) for Gsp1p, involved in nucleartransport 4e-050.00111 S000004794RGM1YMR182CverifiedPutative transcriptional repressor with proline-rich zincfingers; overproduction impairs cell growth 4e-050.00111&radic S000000639YCR043CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene 4e-050.00111 S000002591PLP1YDR183WverifiedProtein with a possible role in folding of beta-tubulin; hassimilarity to phosducins, which are GTPaseinhibitors 4e-050.00111 S000001729ALY1YKR021WuncharacterizedPutative protein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasm 4e-050.00111 S000000459YBR255WUncharacterizedProtein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 4e-050.00111&radic S000003196VID30YGL227WverifiedProtein involved in proteasome-dependent catabolite degradationof fructose-1,6-bisphosphatase (FBPase); shiftsthe balance of nitrogen metabolism toward theproduction of glutamate; localized to thenucleus and the cytoplasm 4e-050.00111&radic S000002554EKI1YDR147WverifiedEthanolamine kinase, primarily responsible forphosphatidylethanolamine synthesis via theCDP-ethanolamine pathway; also exhibits cholinekinase activity 4e-050.00111 S000000384DTR1YBR180WverifiedMultidrug resistance dityrosine transporter of the majorfacilitator superfamily, essential for sporewall synthesis, facilitates the translocationof bisformyl dityrosine through the prosporemembrane 4e-050.00111 S000006320YPR116WUncharacterizedHypothetical protein 4e-050.00111 S000000575YCL073CUncharacterizedProtein of unconfirmed function; displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins; coding sequence 98% identical to that of YKR106W 4e-050.00111&radic S000005863KRE5YOR336WverifiedProtein required for beta-1,6 glucan biosynthesis; mutationsresult in aberrant morphology and severe growthdefects 4e-050.00111 S000006002RPS9AYPL081WverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps9Bp and has similarity to E.coli S4 and rat S9 ribosomal proteins 4e-050.00111 S000002461CDC34YDR054CverifiedUbiquitin-conjugating enzyme or E2; together with Skp1p, Rbx1p,Cdc53p, and an F-box protein, forms aubiquitin-protein ligase called the SCF complexwhich regulates cell cycle progression bytargeting key substrates for degradation 4e-050.00111&radic&radic S000003979YLL056CUncharacterizedPutative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in pleiotropic drug resistance (PDR) phenomenon; YLL056C is not an essential gene 4e-050.00111 S000002216USO1YDL058WverifiedEssential protein involved in intracellular protein transport,coiled-coil protein necessary for transportfrom ER to Golgi; required for assembly of theER-to-Golgi SNARE complex 4e-050.00111&radic S000001038PAU13YHL046CuncharacterizedPutative protein of unknown function; not an essential gene 4e-050.00111 S000003321NNF2YGR089WuncharacterizedProtein that exhibits physical and genetic interactions withRpb8p, which is a subunit of RNA polymerases I,II, and III; computational analysis oflarge-scale protein-protein interaction datasuggests a role in chromosome segregation 4e-050.00111&radic S000004132PUT1YLR142WverifiedProline oxidase, nuclear-encoded mitochondrial protein involvedin utilization of proline as sole nitrogensource; PUT1 transcription is induced by Put3pin the presence of proline and the absence of apreferred nitrogen source 4e-050.00111 S000000812YER010CVerifiedProtein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction 4e-050.00111 S000003026RAD6YGL058WverifiedUbiquitin-conjugating enzyme (E2), involved in postreplicationrepair (with Rad18p), sporulation, telomeresilencing, and ubiquitin-mediated N-end ruleprotein degradation (with Ubr1p) 4e-050.00111&radic S000003978YLL055WUncharacterizedPutative protein of unknown function with similarity to transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLL055W is not an essential gene 4e-050.00111 S000002830SIP1YDR422CverifiedAlternate beta-subunit of the Snf1p kinase complex, may confersubstrate specificity; vacuolar proteincontaining KIS (Kinase-Interacting Sequence)and ASC (Association with Snf1 kinase Complex)domains involved in protein interactions 4e-050.00111 S000004364SUR4YLR372WverifiedElongase, involved in fatty acid and sphingolipid biosynthesis;synthesizes very long chain 20-26-carbon fattyacids from C18-CoA primers; involved inregulation of sphingolipid biosynthesis 4e-050.00111 S000003807TAH11YJR046WverifiedDNA replication licensing factor, required for pre-replicationcomplex assembly 4e-050.00111&radic S000005384YOL024WUncharacterizedHypothetical protein 4e-050.00111&radic S000005832YOR305WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; YOR305W is not an essential gene 4e-050.00111 S000028672YLL066W-BUncharacterizedDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data 4e-050.00111 S000003868YJR107WUncharacterizedPutative protein of unknown function; has sequence or structural similarity to lipases 4e-050.00111 S000002315YDL156WUncharacterizedHypothetical protein 4e-050.00111&radic S000003773YJR012CUncharacterizedPutative protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W 4e-050.00111&radic S000004081YLR091WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR091W is not an esssential gene 4e-050.00111 S000028683YLR406C-AUncharacterizedPutative protein of unknown function 4e-050.00111 S000003581YJL045WUncharacterizedMinor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner 4e-050.00111 S000003849BIR1YJR089WverifiedEssential chromosomal passenger protein involved incoordinating cell cycle events for properchromosome segregation; C-terminal region bindsSli15p, and the middle region, uponphosphorylation, localizes Cbf2p to the spindleat anaphase 4e-050.00111&radic S000002263NSE4YDL105WverifiedNuclear protein that plays a role in the function of theSmc5p-Rhc18p complex 4e-050.00111&radic S000003626DPB11YJL090CverifiedEssential BRCT repeat protein, required on the prereplicativecomplex at replication origins for loading DNApolymerases to initiate DNA synthesis, alsorequired for S/M checkpoint control 4e-050.00111&radic S000002393GYP7YDL234CverifiedGTPase-activating protein for yeast Rab family membersincluding: Ypt7p (most effective), Ypt1p,Ypt31p, and Ypt32p (in vitro); involved invesicle mediated protein trafficking 4e-050.00111 S000006138BMS1YPL217CverifiedEssential conserved nucleolar GTP-binding protein required forsynthesis of 40S ribosomal subunits and forprocessing of the 35S pre-rRNA at sites A0, A1,and A2; interacts with Rcl1p, has similarity toTsr1p 4e-050.00111 S000001902YFR006WUncharacterizedPutative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene 4e-050.00111&radic S000004825EFR3YMR212CverifiedNon-essential protein of unknown function; exhibits syntheticlethal genetic interactions with PHO85; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 4e-050.00111 S000001278SNL1YIL016WverifiedProtein of unknown function proposed to be involved in nuclearpore complex biogenesis and maintenance as wellas protein folding; has similarity to themammalian BAG-1 protein 4e-050.00111 S000002470YDR063WUncharacterizedHypothetical protein 4e-050.00111&radic S000003681SFH5YJL145WverifiedPutative phosphatidylinositol transfer protein (PITP), exhibitsphosphatidylinositol- but notphosphatidylcholine-transfer activity, mainlylocalized to cytosol and microsomes, similar toSec14p; may be PITP regulator rather thanactual PITP 4e-050.00111&radic S000001156BZZ1YHR114WverifiedSH3 domain protein implicated in the regulation of actinpolymerization, able to recruit actinpolymerization machinery through its SH3domains, colocalizes with cortical actinpatches and Las17p, interacts with type Imyosins 4e-050.00111 S000006380BET2YPR176CverifiedBeta subunit of Type II geranylgeranyltransferase required forvesicular transport between the endoplasmicreticulum and the Golgi; provides a membraneattachment moiety to Rab-like proteins Ypt1pand Sec4p 4e-050.00111 S000000184TEL1YBL088CverifiedProtein kinase primarily involved in telomere lengthregulation; contributes to cell cyclecheckpoint control in response to DNA damage;functionally redundant with Mec1p; homolog ofhuman ataxia telangiectasia (ATM) gene 4e-050.00111 S000001202YHR159WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate 4e-050.00111 S000004237IRC20YLR247CuncharacterizedPutative helicase; localized to mitochondria and the nucleus;YLR247C is not an essential gene; null mutantdisplays increased levels of spontaneous Rad52foci 4e-050.00111&radic S000002826RPL12BYDR418WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl12Ap; rpl12a rpl12b doublemutant exhibits slow growth and slowtranslation; has similarity to E. coli L11 andrat L12 ribosomal proteins 4e-050.00111 S000001441MPH1YIR002CverifiedMember of the DEAH family of helicases, functions in anerror-free DNA damage bypass pathway thatinvolves homologous recombination, mutationsconfer a mutator phenotype 4e-050.00111&radic S000005997GPI2YPL076WverifiedProtein involved in the synthesis of N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI), the firstintermediate in the synthesis ofglycosylphosphatidylinositol (GPI) anchors;homologous to the human PIG-C protein 4e-050.00111 S000003997NSE1YLR007WverifiedEssential subunit of the Mms21-Smc5-Smc6 complex; nuclearprotein required for DNA repair and growth 4e-050.00111 S000028510SUS1YBR111W-AverifiedProtein involved in mRNA export coupled transcriptionactivation; component of the SAGA histoneacetylase complex 4e-050.00111 S000002316YDL157CUncharacterizedHypothetical protein 4e-050.00111 S000006038IDI1YPL117CverifiedIsopentenyl diphosphate:dimethylallyl diphosphate isomerase(IPP isomerase), catalyzes an essentialactivation step in the isoprenoid biosyntheticpathway; required for viability 4e-050.00111&radic S000003880JHD2YJR119CuncharacterizedPutative protein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmand nucleus 4e-050.00111&radic S000000407COS111YBR203WverifiedProtein required for resistance to the antifungal drugciclopirox olamine; not related to thesubtelomerically-encoded COS family; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 4e-050.00111 S000000126PET9YBL030CverifiedMajor ADP/ATP carrier of the mitochondrial inner membrane,exchanges cytosolic ADP for mitochondriallysynthesized ATP; required for viability in manycommon lab strains carrying a mutation in thepolymorphic SAL1 gene 4e-050.00111 S000001735BCH2YKR027WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p, from the Golgi to plasma membrane 4e-050.00111 S000000559SPB1YCL054WverifiedAdoMet-dependent methyltransferase involved in rRNA processingand 60S ribosomal subunit maturation;methylates G2922 in the tRNA docking site ofthe large subunit rRNA and in the absence ofsnR52, U2921; suppressor of PAB1 mutants 4e-050.00111&radic&radic S000000811NTF2YER009WverifiedNuclear envelope protein, interacts with GDP-bound Gsp1p andwith proteins of the nuclear pore to transportGsp1p into the nucleus where it is an essentialplayer in nucleocytoplasmic transport 4e-050.00111 S000001213NMD3YHR170WverifiedProtein involved in nuclear export of the large ribosomalsubunit; acts as a Crm1p-dependent adapterprotein for export of nascent ribosomalsubunits through the nuclear pore complex 4e-050.00111 S000001709VPS1YKR001CverifiedGTPase required for vacuolar protein sorting, functions inactin cytoskeleton organization via itsinteraction with Sla1p; required for lateGolgi-retention of some proteins includingKex2p; involved in regulating peroxisomebiogenesis 4e-050.00111 S000005335POP2YNR052CverifiedRNase of the DEDD superfamily, subunit of the Ccr4-Not complexthat mediates 3' to 5' mRNA deadenylation 4e-050.00111 S000001470DAL7YIR031CverifiedMalate synthase, role in allantoin degradation unknown;expression sensitive to nitrogen cataboliterepression and induced by allophanate, anintermediate in allantoin degradation 4e-050.00111 S000000598CIT2YCR005CverifiedCitrate synthase, catalyzes the condensation of acetyl coenzymeA and oxaloacetate to form citrate, peroxisomalisozyme involved in glyoxylate cycle;expression is controlled by Rtg1p and Rtg2ptranscription factors 4e-050.00111 S000005888PRT1YOR361CverifiedSubunit of the core complex of translation initiation factor3(eIF3), essential for translation; part of asubcomplex (Prt1p-Rpg1p-Nip1p) that stimulatesbinding of mRNA and tRNA(i)Met to ribosomes 4e-050.00111 S000003228YPS5YGL259WuncharacterizedProtein with similarity to GPI-anchored aspartic proteases suchas Yap1p and Yap3p 4e-050.00111 S000002630YDR222WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 4e-050.00111 S000004972CRZ1YNL027WverifiedTranscription factor that activates transcription of genesinvolved in stress response; nuclearlocalization is positively regulated bycalcineurin-mediated dephosphorylation 4e-050.00111 S000007239YER138W-AUncharacterizedPutative protein of unknown function 4e-050.00111 S000004725ASI1YMR119WverifiedPutative integral membrane E3 ubiquitin ligase; acts with Asi2pand Asi3p to ensure the fidelity of SPS-sensorsignalling by maintaining the dormant repressedstate of gene expression in the absence ofinducing signals 4e-050.00111 S000001016RIM4YHL024WverifiedPutative RNA-binding protein required for the expression ofearly and middle sporulation genes 4e-050.00111 S000005225HCH1YNL281WverifiedHeat shock protein regulator that binds to Hsp90p and maystimulate ATPase activity; originallyidentified as a high-copy number suppressor ofa HSP90 loss-of-function mutation; GFP-fusionprotein localizes to the cytoplasm and nucleus 4e-050.00111&radic S000002382HBT1YDL223CverifiedSubstrate of the Hub1p ubiquitin-like protein that localizes tothe shmoo tip (mating projection); mutants aredefective for mating projection formation,thereby implicating Hbt1p in polarized cellmorphogenesis 4e-050.00111 S000000076YAR028WUncharacterizedPutative integral membrane protein, member of DUP240 gene family 4e-050.00111 S000003052GUP1YGL084CverifiedPlasma membrane protein with a possible role in proton symportof glycerol; member of the MBOAT family ofputative membrane-bound O-acyltransferases 4e-050.00111 S000028565YLR157C-CUncharacterizedPutative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-4 4e-050.00111 S000002277YDL119CUncharacterizedPutative mitochondrial transport protein; localizes to the mitochondrion; YDL119C is not an essential gene 4e-050.00111 S000004981NCE103YNL036WverifiedCarbonic anhydrase; poorly transcribed under aerobic conditionsand at an undetectable level under anaerobicconditions; involved in non-classical proteinexport pathway 4e-050.00111&radic S000003695HSP150YJL159WverifiedO-mannosylated heat shock protein that is secreted andcovalently attached to the cell wall viabeta-1,3-glucan and disulfide bridges; requiredfor cell wall stability; induced by heat shock,oxidative stress, and nitrogen limitation 4e-050.00111 S000005040RPS7BYNL096CverifiedProtein component of the small (40S) ribosomal subunit, nearlyidentical to Rps7Ap; interacts with Kti11p;deletion causes hypersensitivity to zymocin;has similarity to rat S7 and Xenopus S8ribosomal proteins 4e-050.00111 S000002736SKP1YDR328CverifiedEvolutionarily conserved kinetochore protein that is part ofmultiple protein complexes, including the SCFubiquitin ligase complex, the CBF3 complex thatbinds centromeric DNA, and the RAVE complexthat regulates assembly of the V-ATPase 4e-050.00111&radic S000005910FIT3YOR383CverifiedMannoprotein that is incorporated into the cell wall via aglycosylphosphatidylinositol (GPI) anchor,involved in the retention of siderophore-ironin the cell wall 4e-050.00111 S000001397VHS2YIL135CverifiedCytoplasmic protein of unknown function; identified as ahigh-copy suppressor of the synthetic lethalityof a sis2 sit4 double mutant, suggesting a rolein G1/S phase progression; similar to Mlf3p 4e-050.00111 S000005379YOL019WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole 4e-050.00111 S000003716ATP12YJL180CverifiedConserved protein required for assembly of alpha and betasubunits into the F1 sector of mitochondrialF1F0 ATP synthase; mutation of human ATP12reduces active ATP synthase levels and isassociated with the disorder ATPAF2 deficiency 4e-050.00111 S000000344IRA1YBR140CverifiedGTPase-activating protein that negatively regulates RAS byconverting it from the GTP- to the GDP-boundinactive form, required for reducing cAMPlevels under nutrient limiting conditions,mediates membrane association of adenylatecyclase 4e-050.00111 S000002451HEM13YDR044WverifiedCoproporphyrinogen III oxidase, an oxygen requiring enzyme thatcatalyzes the sixth step in the hemebiosynthetic pathway; localizes to themitochondrial inner membrane; transcription isrepressed by oxygen and heme (via Rox1p andHap1p) 4e-050.00111&radic S000001859MOB2YFL034C-BverifiedComponent of the RAM signaling network, localizes and activatesthe Ace2p in the daughter cell nucleus todirect daughter cell-specific transcription ofseveral genes involved in cell separation;Mob1p-like protein 4e-050.00111 S000001733RPC37YKR025WverifiedRNA polymerase III subunit C37 4e-050.00111&radic S000002894VPS60YDR486CverifiedCytoplasmic and vacuolar membrane protein involved in lateendosome to vacuole transport; required fornormal filament maturation during pseudohyphalgrowth; may function in targeting specificcargo proteins for degradation 4e-050.00111 S000005805RFM1YOR279CverifiedDNA-binding protein required for vegetative repression ofmiddle sporulation genes; specificity factorthat directs the Hst1p histone deacetylase tosome of the promoters regulated by Sum1p;involved in telomere maintenance 4e-050.00111&radic S000005499CDC33YOL139CverifiedCytoplasmic mRNA cap binding protein; the eIF4E-cap complex isresponsible for mediating cap-dependent mRNAtranslation via interactions with thetranslation initiation factor eIF4G (Tif4631por Tif4632p) 4e-050.00111 S000003814BFA1YJR053WverifiedComponent of the GTPase-activating Bfa1p-Bub2p complex involvedin multiple cell cycle checkpoint pathways thatcontrol exit from mitosis 4e-050.00111 S000003808ANB1YJR047CverifiedTranslation initiation factor eIF-5A, promotes formation of thefirst peptide bond; similar to and functionallyredundant with Hyp2p; undergoes an essentialhypusination modification; expressed underanaerobic conditions 4e-050.00111 S000004097REX3YLR107WverifiedRNA exonuclease; required for maturation of the RNA componentof RNase MRP; functions redundantly with Rnh70pand Rex2p in processing of U5 snRNA and RNase PRNA; member of RNase D family of exonucleases 4e-050.00111&radic S000005245RPL18BYNL301CverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl18Ap and has similarity to ratL18 ribosomal protein 4e-050.00111 S000003590TIM54YJL054WverifiedComponent of the mitochondrial Tim54p-Tim22p complex involvedin insertion of polytopic proteins into theinner membrane 4e-050.00111 S000004003GAT3YLR013WverifiedProtein containing GATA family zinc finger motifs 4e-050.00111 S000001365DPH1YIL103WverifiedProtein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p,for synthesis of diphthamide, which is amodified histidine residue of translationelongation factor 2 (Eft1p or Eft2p); may actin a complex with Dph2p and Kti11p 4e-050.00111 S000006208YPR004CVerifiedElectron transfer flavoprotein complex subunit ETF-alpha; contains a FAD binding domain; interacts with YFH1, the yeast frataxin homolog 4e-050.00111 S000004762NUP53YMR153WverifiedSubunit of the nuclear pore complex (NPC), interacts withkaryopherin Kap121p or with Nup170p viaoverlapping regions of Nup53p, involved inactivation of the spindle checkpoint mediatedby the Mad1p-Mad2p complex 4e-050.00111 S000003741YJL206CUncharacterizedPutative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment 4e-050.00111 S000003942KNS1YLL019CverifiedNonessential putative protein kinase of unknown cellular role;member of the LAMMER family of protein kinases,which are serine/threonine kinases also capableof phosphorylating tyrosine residues 4e-050.00111 S000005446ADH1YOL086CverifiedAlcohol dehydrogenase, fermentative isozyme active as homo- orheterotetramers; required for the reduction ofacetaldehyde to ethanol, the last step in theglycolytic pathway 4e-050.00111 S000004718MED11YMR112CverifiedEssential subunit of the RNA polymerase II mediator complex;associates with core polymerase subunits toform the RNA polymerase II holoenzyme 4e-050.00111&radic S000000892TRP2YER090WverifiedAnthranilate synthase, catalyzes the initial step of tryptophanbiosynthesis, forms multifunctionalhetero-oligomeric anthranilatesynthase:indole-3-glycerol phosphate synthaseenzyme complex with Trp3p 4e-050.00111 S000028654YHR214C-EUncharacterizedIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 4e-050.00111 S000000671ERS1YCR075CverifiedProtein with similarity to human cystinosin, which is aH(+)-driven transporter involved in L-cystineexport from lysosomes and implicated in thedisease cystinosis; contains seventransmembrane domains 4e-050.00111 S000006008YDC1YPL087WverifiedAlkaline dihydroceramidase, involved in sphingolipidmetabolism; preferentially hydrolyzesdihydroceramide to a free fatty acid anddihydrosphingosine; has a minor reverseactivity 4e-050.00111 S000003362YGR130CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) 4e-050.00111 S000003708CPS1YJL172WverifiedVacuolar carboxypeptidase yscS; expression is induced underlow-nitrogen conditions 4e-050.00111 S000002454HEM12YDR047WverifiedUroporphyrinogen decarboxylase, catalyzes the fifth step in theheme biosynthetic pathway; localizes to boththe cytoplasm and nucleus; activity inhibitedby Cu2+, Zn2+, Fe2+, Fe3+ andsulfhydryl-specific reagents 4e-050.00111&radic S000000902UBC6YER100WverifiedUbiquitin-conjugating enzyme involved in ER-associated proteindegradation; located at the cytosolic side ofthe ER membrane; tail region contains atransmembrane segment at the C-terminus;substrate of the ubiquitin-proteasome pathway 4e-050.00111 S000028827YGR035W-AUncharacterizedPutative protein of unknown function 4e-050.00111 S000003969RNP1YLL046CverifiedRibonucleoprotein that contains two RNA recognition motifs(RRM) 4e-050.00111 S000001185RPC10YHR143W-AverifiedRNA polymerase subunit, found in RNA polymerase complexes I,II, and III 4e-050.00111 S000003449CCH1YGR217WverifiedVoltage-gated high-affinity calcium channel involved in calciuminflux in response to some environmentalstresses as well as exposure to matingpheromones; interacts and co-localizes withMid1p, suggesting Cch1p and Mid1p functiontogether 4e-050.00111 S000006249MNI2YPR045CuncharacterizedDeletion mutant is synthetically lethal with MEN mutants 4e-050.00111&radic S000004629YMR027WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene 4e-050.00111&radic S000006104YPL183CUncharacterizedHypothetical protein 4e-050.00111 S000005275AAD14YNL331CverifiedPutative aryl-alcohol dehydrogenase with similarity to P.chrysosporium aryl-alcohol dehydrogenase;mutational analysis has not yet revealed aphysiological role 4e-050.00111 S000004342ORM2YLR350WverifiedEvolutionarily conserved protein with similarity to Orm1p,required for resistance to agents that inducethe unfolded protein response; human orthologis located in the endoplasmic reticulum 4e-050.00111 S000002614EBS1YDR206WverifiedCytoplasmic protein of unknown function, involved in inhibitionof translation; interacts with cap bindingprotein Cdc33; contains an RNA recognitionmotif and similarity to Est1p; mRNA abundanceregulated by mRNA decay factors 4e-050.00111 S000003995SSL1YLR005WverifiedComponent of the core form of RNA polymerase transcriptionfactor TFIIH, which has both protein kinase andDNA-dependent ATPase/helicase activities and isessential for transcription and nucleotideexcision repair; interacts with Tfb4p 4e-050.00111&radic S000005837NOP58YOR310CverifiedProtein involved in pre-rRNA processing, 18S rRNA synthesis,and snoRNA synthesis; component of the smallsubunit processome complex, which is requiredfor processing of pre-18S rRNA 4e-050.00111 S000005558HMS1YOR032CverifiedBasic helix-loop-helix (bHLH) protein with similarity tomyc-family transcription factors;overexpression confers hyperfilamentous growthand suppresses the pseudohyphal filamentationdefect of a diploid mep1 mep2 homozygous nullmutant 4e-050.00111 S000005237MSB3YNL293WverifiedGTPase-activating protein for Sec4p and several other RabGTPases, regulates exocytosis via its action onSec4p, also required for proper actinorganization; similar to Msb4p; both Msb3p andMsb4p localize to sites of polarized growth 4e-050.00111 S000002947YDR539WUncharacterizedHypothetical protein 4e-050.00111 S000002580ARG82YDR173CverifiedInositol polyphosphate multikinase (IPMK), sequentiallyphosphorylates Ins(1,4,5)P3 to formIns(1,3,4,5,6)P5; putative nuclear lipidinositol PI3-kinase; regulates arginine-,phosphate-, and nitrogen-responsive genes 4e-050.00111&radic S000028514YMR194C-BUncharacterizedPutative protein of unknown function 4e-050.00111 S000002976PMA1YGL008CverifiedPlasma membrane H+-ATPase, pumps protons out of the cell; majorregulator of cytoplasmic pH and plasma membranepotential; part of the P2 subgroup ofcation-transporting ATPases 4e-050.00111&radic&radic S000001087YHR045WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum 4e-050.00111&radic S000003736ACO2YJL200CuncharacterizedPutative mitochondrial aconitase isozyme; similarity to Aco1p,an aconitase required for the TCA cycle;expression induced during growth on glucose, byamino acid starvation via Gcn4p, and repressedon ethanol 4e-050.00111 S000002294RDI1YDL135CverifiedRho GDP dissociation inhibitor involved in the localization andregulation of Cdc42p 4e-050.00111&radic S000004842RRP5YMR229CverifiedProtein required for the synthesis of both 18S and 5.8S rRNA;C-terminal region is crucial for the formationof 18S rRNA and N-terminal region is requiredfor the 5.8S rRNA; component of small ribosomalsubunit (SSU) processosome 4e-050.00111 S000006050TAF14YPL129WverifiedSubunit (30 kDa) of TFIID, TFIIF, and SWI/SNF complexes,involved in RNA polymerase II transcriptioninitiation and in chromatin modification,contains a YEATS domain 4e-050.00111&radic S000000953UBP3YER151CverifiedUbiquitin-specific protease that interacts with Bre5p toco-regulate anterograde and retrogradetransport between endoplasmic reticulum andGolgi compartments; inhibitor of genesilencing; cleaves ubiquitin fusions but notpolyubiquitin 4e-050.00111 S000005648PFY1YOR122CverifiedProfilin, actin- and phosphatidylinositol4,5-bisphosphate-binding protein, plays a rolein cytoskeleton organization, required fornormal timing of actin polymerization inresponse to thermal stress; localizes to plasmamembrane and cytosol 4e-050.00111&radic S000002432RPS11AYDR025WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps11Bp and has similarity to E.coli S17 and rat S11 ribosomal proteins 4e-050.00111 S000003312TWF1YGR080WverifiedTwinfilin, highly conserved actin monomer-sequestering proteininvolved in regulation of the cortical actincytoskeleton, composed of two cofilin-likeregions, localizes actin monomers to sites ofrapid filament assembly 4e-050.00111 S000004456YLR464WUncharacterizedPutative protein of unknown function with similarity to helicases; YLR464W contains an intron and overlaps the verified gene YRF1-4/YLR466W and two dubious ORFs YLR463C and YLR465C 4e-050.00111 S000003195YGL226WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion 4e-050.00111 S000003648MDV1YJL112WverifiedPeripheral protein of the cytosolic face of the mitochondrialouter membrane, required for mitochondrialfission; interacts with Fis1p and with thedynamin-related GTPase Dnm1p; contains WDrepeats 4e-050.00111 S000001351YIL089WUncharacterizedHypothetical protein 4e-050.00111&radic S000006020FMP14YPL099CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.00111 S000001115OSH3YHR073WverifiedMember of an oxysterol-binding protein family with sevenmembers in S. cerevisiae; family members haveoverlapping, redundant functions in sterolmetabolism and collectively perform a functionessential for viability 4e-050.00111 S000005096YNL152WUncharacterizedProtein required for cell viability 4e-050.00111 S000001003PRS3YHL011Cverified5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involvedin nucleotide, histidine, and tryptophanbiosynthesis; one of a five related enzymes,which are active as heteromultimeric complexes 4e-050.00111 S000000118PIM1YBL022CverifiedMitochondrial ATP-dependent protease involved inintramitochondrial proteolysis; involved indegradation of misfolded proteins inmitochondria; required for bigenesis andmaintenance of mitochondria 4e-050.00111&radic S000005420GPD2YOL059WverifiedNAD-dependent glycerol 3-phosphate dehydrogenase, homolog ofGpd1p, expression is controlled by anoxygen-independent signaling pathway requiredto regulate metabolism under anoxic conditions;located in cytosol and mitochondria 4e-050.00111&radic S000005196MRPL17YNL252CverifiedMitochondrial ribosomal protein of the large subunit 4e-050.00111 S000006007ELP3YPL086CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;exhibits histone acetyltransferase activitythat is directed to histones H3 and H4;disruption confers resistance to K. lactiszymotoxin 4e-050.00111 S000002358YDL199CUncharacterizedPutative protein of unknown function with sequence similarity to members of the sugar permease family 4e-050.00111 S000001501SWD2YKL018WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lys 4 and is involved intelomeric silencing; subunit of CPF (cleavageand polyadenylation factor), a complex involvedin RNAP II transcription termination 4e-050.00111 S000003878STE24YJR117WverifiedHighly conserved zinc metalloprotease that functions in twosteps of a-factor maturation, C-terminal CAAXproteolysis and the first step of N-terminalproteolytic processing; contains multipletransmembrane spans 4e-050.00111 S000003264GSC2YGR032WverifiedCatalytic subunit of 1,3-beta-glucan synthase, involved information of the inner layer of the spore wall;activity positively regulated by Rho1p andnegatively by Smk1p; has similarity to analternate catalytic subunit, Fks1p (Gsc1p) 4e-050.00111 S000001086DOG1YHR044Cverified2-deoxyglucose-6-phosphate phosphatase, similar to Dog2p,member of a family of low molecular weightphosphatases; confers 2-deoxyglucose resistancewhen overexpressed, in vivo substrate has notyet been identified 4e-050.00111&radic S000000059FLO9YAL063CverifiedLectin-like protein with similarity to Flo1p, thought to beexpressed and involved in flocculation 4e-050.00111 S000001543FBA1YKL060CverifiedFructose 1,6-bisphosphate aldolase, required for glycolysis andgluconeogenesis; catalyzes conversion offructose 1,6 bisphosphate to glyceraldehyde-3-Pand dihydroxyacetone-P; locates tomitochondrial outer surface upon oxidativestress 4e-050.00111 S000000769YEL043WUncharacterizedPredicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; contains fibronectin type III domain fold 4e-050.00111 S000005367CSI2YOL007CuncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mother side ofthe bud neck and the vacuole; YOL007C is not anessential gene 4e-050.00111 S000000151YBL055CUncharacterizedHypothetical protein 4e-050.00111 S000004778CEP3YMR168CverifiedEssential kinetochore protein, component of the CBF3 complexthat binds the CDEIII region of the centromere;contains an N-terminal Zn2Cys6 type zinc fingerdomain, a C-terminal acidic domain, and aputative coiled coil dimerization domain 4e-050.00111 S000004552YML087CUncharacterizedHypothetical protein 4e-050.00111 S000000218YBR014CUncharacterizedHypothetical protein 4e-050.00111 S000005904ATF1YOR377WverifiedAlcohol acetyltransferase with potential roles in lipid andsterol metabolism; responsible for the majorpart of volatile acetate ester productionduring fermentation 4e-050.00111 S000004481OST6YML019WverifiedSubunit of the oligosaccharyltransferase complex of the ERlumen, which catalyzes asparagine-linkedglycosylation of newly synthesized proteins;similar to and partially functionally redundantwith Ost3p 4e-050.00111 S000001107RRP3YHR065CverifiedProtein involved in rRNA processing; required for maturation ofthe 35S primary transcript of pre-rRNA and forcleavage leading to mature 18S rRNA; homologousto eIF-4a, which is a DEAD box RNA-dependentATPase with helicase activity 4e-050.00111&radic S000003968RPL8BYLL045CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Ap and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 4e-050.00111 S000000431MCX1YBR227CverifiedMitochondrial ATP-binding protein, possibly a mitochondrialchaperone with non-proteolytic function;similar to bacterial ClpX proteins 4e-050.00111 S000000868YER066WUncharacterizedHypothetical protein 4e-050.00111 S000006131SRP72YPL210CverifiedCore component of the signal recognition particle (SRP)ribonucleoprotein (RNP) complex that functionsin targeting nascent secretory proteins to theendoplasmic reticulum (ER) membrane 4e-050.00111 S000002609SPC19YDR201WverifiedEssential subunit of the Dam1 complex (aka DASH complex),couples kinetochores to the force produced byMT depolymerization thereby aiding inchromosome segregation; also localized tonuclear side of spindle pole body 4e-050.00111 S000003647CCT7YJL111WverifiedSubunit of the cytosolic chaperonin Cct ring complex, relatedto Tcp1p, required for the assembly of actinand tubulins in vivo 4e-050.00111 S000003409ATF2YGR177CverifiedAlcohol acetyltransferase, may play a role in steroiddetoxification; forms volatile esters duringfermentation, which is important in brewing 4e-050.00111 S000003099SNT2YGL131CverifiedDNA binding protein with similarity to the S. pombe Snt2protein 4e-050.00111&radic&radic S000004049REX2YLR059CverifiedRNA exonuclease, required for U4 snRNA maturation; functionsredundantly with Rnh70p in 5.8S rRNAmaturation, and with Rnh70p and Rex3p inprocessing of U5 snRNA and RNase P RNA; memberof RNase D family of exonucleases 4e-050.00111&radic S000000684ABP1YCR088WverifiedActin-binding protein of the cortical actin cytoskeleton,important for activation of the Arp2/3 complexthat plays a key role actin in cytoskeletonorganization 4e-050.00111 S000004592PHO84YML123CverifiedHigh-affinity inorganic phosphate (Pi) transporter andlow-affinity manganese transporter; regulatedby Pho4p and Spt7p; mutation confers resistanceto arsenate; exit from the ER during maturationrequires Pho86p 4e-050.00111 S000005985CWC27YPL064CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to S. pombeCwf27p 4e-050.00111&radic S000005297YNR014WUncharacterizedHypothetical protein 4e-050.00111 S000002342YDL183CUncharacterizedPutative protein of unknown function; YBR063C is not an essential gene 4e-050.00111&radic S000000208GPI18YBR004CverifiedFunctional ortholog of human PIG-V, which is amannosyltransferase that transfers the secondmannose in glycosylphosphatidylinositolbiosynthesis; the authentic, non-tagged proteinwas localized to mitochondria 4e-050.00111&radic S000007237ISD11YER048W-AverifiedProtein required for mitochondrial iron-sulfur clusterbiosynthesis 4e-050.00111 S000002376TIM22YDL217CverifiedComponent of the mitochondrial Tim54p-Tim22p complex involvedin insertion of polytopic proteins into theinner membrane 4e-050.00111&radic S000001466DAL1YIR027CverifiedAllantoinase, converts allantoin to allantoate in the firststep of allantoin degradation; expressionsensitive to nitrogen catabolite repression 4e-050.00111 S000029675REP1R0020CverifiedMaster regulator that acts in concert with Rep2p to regulatetranscript levels of the FLP1 gene thatpromotes plasmid copy amplification;autoregulates levels of its own transcript 4e-050.00111 S000003842EAF6YJR082CuncharacterizedEsa1p-associated factor, subunit of the NuA4 acetyltransferasecomplex 4e-050.00111&radic&radic S000001201KEL1YHR158CverifiedProtein required for proper cell fusion and cell morphology;functions in a complex with Kel2p to negativelyregulate mitotic exit, interacts with Tem1p andLte1p; localizes to regions of polarizedgrowth; potential Cdc28p substrate 4e-050.00111 S000004690YMR085WUncharacterizedHypothetical protein 4e-050.00111&radic S000003661GCD14YJL125CverifiedSubunit of tRNA (1-methyladenosine) methyltransferase, withGcd10p, required for the modification of theadenine at position 58 in tRNAs, especiallytRNAi-Met; first identified as a negativeregulator of GCN4 expression 4e-050.00111&radic&radic S000001151CTM1YHR109WverifiedCytochrome c lysine methyltransferase, trimethylates residue 72of apo-cytochrome c (Cyc1p) in the cytosol; notrequired for normal respiratory growth 4e-050.00111 S000001715MEH1YKR007WverifiedComponent of the EGO complex, which is involved in theregulation of microautophagy, and of the GSEcomplex, which is required for proper sortingof amino acid permease Gap1p; loss results in adefect in vacuolar acidification 4e-050.00111 S000000877PTP3YER075CverifiedPhosphotyrosine-specific protein phosphatase involved in theinactivation of mitogen-activated proteinkinase (MAPK) during osmolarity sensing;dephosporylates Hog1p MAPK and regulates itslocalization; localized to the cytoplasm 4e-050.00111 S000001937ERJ5YFR041CuncharacterizedType I membrane protein with a J domain is required to preservethe folding capacity of the endoplasmicreticulum; loss of the non-essential ERJ5 geneleads to a constitutively induced unfoldedprotein response 4e-050.00111&radic S000004105CFT2YLR115WverifiedSubunit of the mRNA cleavage and polyadenlylation factor (CPF);required for pre-mRNA cleavage, polyadenylationand poly(A) site recognition, 43% similaritywith the mammalian CPSF-100 protein. 4e-050.00111&radic S000004786PAI3YMR174CverifiedCytoplasmic proteinase A inhibitor, dependent on Pbs2p andHog1p protein kinases for osmotic induction;intrinsically unstructured, N-terminal halfbecomes ordered in the active site ofproteinase A upon contact 4e-050.00111&radic S000003236PEX31YGR004WverifiedPeroxisomal integral membrane protein, involved in negativeregulation of peroxisome size; partiallyfunctionally redundant with Pex30p and Pex32p;probably acts at a step downstream of stepsmediated by Pex28p and Pex29p 4e-050.00111 S000006190KAR9YPL269WverifiedKaryogamy protein required for correct positioning of themitotic spindle and for orienting cytoplasmicmicrotubules, localizes at the shmoo tip inmating cells and at the tip of the growing budin small-budded cells through anaphase 4e-050.00111 S000006164SRP68YPL243WverifiedCore component of the signal recognition particle (SRP)ribonucleoprotein (RNP) complex that functionsin targeting nascent secretory proteins to theendoplasmic reticulum (ER) membrane 4e-050.00111 S000001759YKR051WUncharacterizedHypothetical protein 4e-050.00111 S000001000YHL008CUncharacterizedPutative protein of unknown function, does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 4e-050.00111&radic S000000160PRX1YBL064CverifiedMitochondrial peroxiredoxin (1-Cys Prx) with thioredoxinperoxidase activity, has a role in reduction ofhydroperoxides; induced during respiratorygrowth and under conditions of oxidative stress 4e-050.00111 S000004233YLR243WUncharacterizedPutative protein of unknown function; YLR243W is an essential gene 4e-050.00111 S000028534YBR196C-AUncharacterizedPutative protein of unknown function; identified by fungal homology and RT-PCR 4e-050.00111 S000002494RRP1YDR087CverifiedEssential evolutionarily conserved nucleolar protein necessaryfor biogenesis of 60S ribosomal subunits andprocessing of pre-rRNAs to mature rRNAs,associated with several distinct 66Spre-ribosomal particles 4e-050.00111&radic S000002151YBL111CUncharacterizedHelicase-like protein encoded within the telomeric Y' element 4e-050.00111 S000004305SPH1YLR313CverifiedProtein involved in shmoo formation and bipolar bud siteselection; homologous to Spa2p, localizes tosites of polarized growth in a cell cycledependent- and Spa2p-dependent manner,interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p 4e-050.00111&radic&radic S000003366CAF130YGR134WverifiedPart of the evolutionarily-conserved CCR4-NOT transcriptionalregulatory complex involved in controlling mRNAinitiation, elongation, and degradation 4e-050.00111 S000006108MF(ALPHA)1YPL187WverifiedMating pheromone alpha-factor, made by alpha cells; interactswith mating type a cells to induce cell cyclearrest and other responses leading to mating;also encoded by MF(ALPHA)2, although MF(ALPHA)1produces most alpha-factor 4e-050.00111 S000003300YGR068CUncharacterizedPutative protein of unknown function; YGR068C is not an essential gene. 4e-050.00111 S000028508VMA9YCL005W-AverifiedVacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex;essential for vacuolar acidification; interactswith the V-ATPase assembly factor Vma21p in theER; involved in V0 biogenesis 4e-050.00111 S000002711RSC3YDR303CverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; essential gene required forregulation of ribosomal protein genes and thecell wall/stress response; highly similar toRsc30p 4e-050.00111&radic S000007651YOS1YER074W-AverifiedIntegral membrane protein required for ER to Golgi transport;localized to the Golgi, the ER, and COPIIvesicles; interacts with Yip1p and Yif1p 4e-050.00111 S000005902GDH1YOR375CverifiedNADP(+)-dependent glutamate dehydrogenase, synthesizesglutamate from ammonia and alpha-ketoglutarate;rate of alpha-ketoglutarate utilization differsfrom Gdh3p; expression regulated by nitrogenand carbon sources 4e-050.00111&radic S000028541YDR194W-AUncharacterizedPutative protein of unknown function; identified by fungal homology and RT-PCR 4e-050.00111 S000006259SEC8YPR055WverifiedEssential 121kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 4e-050.00111&radic&radic S000004744GAT2YMR136WverifiedProtein containing GATA family zinc finger motifs; similar toGln3p and Dal80p; expression repressed byleucine 4e-050.00111 S000000499PCA1YBR295WverifiedCadmium transporting P-type ATPase; may also have a role incopper and iron homeostasis; S288C and mostother lab strains contain a G970R mutationwhich eliminates normal cadmium transportfunction 4e-050.00111 S000000801YEL075CUncharacterizedHypothetical protein 4e-050.00111 S000005977YPL056CUncharacterizedHypothetical protein 4e-050.00111 S000004223EST1YLR233CverifiedTLC1 RNA-associated factor involved in telomere lengthregulation as the recruitment subunit of thetelomerase holoenzyme, has a possible role inactivating Est2p-TLC1-RNA bound to the telomere 4e-050.00111 S000002309RPC53YDL150WverifiedRNA polymerase III subunit C53 4e-050.00111&radic S000001227SSP1YHR184WverifiedProtein involved in the control of meiotic nuclear division andcoordination of meiosis with spore formation;transcription is induced midway through meiosis 4e-050.00111 S000003962UBI4YLL039CverifiedUbiquitin, becomes conjugated to proteins, marking them forselective degradation via the ubiquitin-26Sproteasome system; essential for the cellularstress response 4e-050.00111&radic S000005911FRE5YOR384WverifiedPutative ferric reductase with similarity to Fre2p; expressioninduced by low iron levels; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 4e-050.00111 S000003442YGR210CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 4e-050.00111 S000006021ATG21YPL100WverifiedPhosphatidylinositol 3,5-bisphosphate-binding protein requiredfor maturation of pro-aminopeptidase I,predicted to fold as a seven-bladedbeta-propeller; displays punctate cytoplasmiclocalization 4e-050.00111 S000000183RPL23AYBL087CverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl23Bp and has similarity to E.coli L14 and rat L23 ribosomal proteins 4e-050.00111&radic S000003331TEL2YGR099WverifiedEssential DNA-binding protein specific to single-stranded yeasttelomeric DNA repeats, required for telomerelength regulation and telomere position effect 4e-050.00111 S000004332RPP0YLR340WverifiedConserved ribosomal protein P0 similar to rat P0, human P0, andE. coli L10e; shown to be phosphorylated onserine 302 4e-050.00111 S000003733UBP12YJL197WverifiedUbiquitin-specific protease present in the nucleus andcytoplasm that cleaves ubiquitin fromubiquitinated proteins 4e-050.00111&radic S000004751RPS16AYMR143WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps16Bp and has similarity to E.coli S9 and rat S16 ribosomal proteins 4e-050.00111 S000004218ECM22YLR228CverifiedSterol regulatory element binding protein, regulatestranscription of the sterol biosynthetic genesERG2 and ERG3; member of the fungus-specificZn[2]-Cys[6] binuclear cluster family oftranscription factors; homologous to Upc2p 4e-050.00111&radic S000000556LRE1YCL051WverifiedProtein involved in control of cell wall structure and stressresponse; inhibits Cbk1p protein kinaseactivity; overproduction confers resistance tocell-wall degrading enzymes 4e-050.00111 S000003225ADH4YGL256WverifiedAlcohol dehydrogenase type IV, dimeric enzyme demonstrated tobe zinc-dependent despite sequence similarityto iron-activated alcohol dehydrogenases;transcription is induced in response to zincdeficiency; alcohol dehydrogenase isoenzyme IV 4e-050.00111 S000000929LCP5YER127WverifiedEssential protein involved in maturation of 18S rRNA; depletionleads to inhibited pre-rRNA processing andreduced polysome levels; localizes primarily tothe nucleolus 4e-050.00111 S000002297RGT2YDL138WverifiedPlasma membrane glucose receptor, highly similar to Snf3p; bothRgt2p and Snf3p serve as transmembrane glucosesensors generating an intracellular signal thatinduces expression of glucose transporter (HXT)genes 4e-050.00111 S000000028SNC1YAL030WverifiedVesicle membrane receptor protein (v-SNARE) involved in thefusion between Golgi-derived secretory vesicleswith the plasma membrane; proposed to beinvolved in endocytosis; member of thesynaptobrevin/VAMP family of R-type v-SNAREproteins 4e-050.00111 S000001339YIL077CUncharacterizedHypothetical protein 4e-050.00111 S000002651PRP28YDR243CverifiedRNA helicase in the DEAD-box family, involved in RNAisomerization at the 5' splice site 4e-050.00111&radic S000001322YIL060WUncharacterizedPutative protein of unknown function; mutant accumulates less glycogen than does wild type; YIL060W is not an essential gene 4e-050.00111&radic&radic S000000433ROT2YBR229CverifiedGlucosidase II catalytic subunit required for normal cell wallsynthesis; mutations in rot2 suppress tor2mutations, and are synthetically lethal withrot1 mutations 4e-050.00111 S000007588YDL085C-AUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 4e-050.00111&radic S000003511SCW4YGR279CverifiedCell wall protein with similarity to glucanases; scw4 scw10double mutants exhibit defects in mating 4e-050.00111 S000028848YMR175W-AUncharacterizedPutative protein of unknown function 4e-050.00111 S000002477FMP16YDR070CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.00111 S000001876LPD1YFL018CverifiedDihydrolipoamide dehydrogenase, the lipoamide dehydrogenasecomponent (E3) of the pyruvate dehydrogenaseand 2-oxoglutarate dehydrogenase multi-enzymecomplexes 4e-050.00111 S000001467DAL4YIR028WverifiedAllantoin permease; expression sensitive to nitrogen cataboliterepression and induced by allophanate, anintermediate in allantoin degradation 4e-050.00111 S000002635SIR4YDR227WverifiedSilent information regulator that, together with SIR2 and SIR3,is involved in assembly of silent chromatindomains at telomeres and the silent mating-typeloci; potentially phosphorylated by Cdc28p;some alleles of SIR4 prolong lifespan 4e-050.00111 S000003973COF1YLL050CverifiedCofilin, promotes actin filament depolarization in apH-dependent manner; binds both actin monomersand filaments and severs filaments , thought tobe regulated by phosphorylation at SER4,ubiquitous and essential in eukaryotes 4e-050.00111 S000002349UFD2YDL190CverifiedUbiquitin chain assembly factor (E4) that cooperates with aubiquitin-activating enzyme (E1), aubiquitin-conjugating enzyme (E2), and aubiquitin protein ligase (E3) to conjugateubiquitin to substrates; also functions as an 4e-050.00111&radic&radic S000005853MYO2YOR326WverifiedOne of two type V myosin motors (along with MYO4) involved inactin-based transport of cargos; required forthe polarized delivery of secretory vesicles,the vacuole, late Golgi elements, peroxisomes,and the mitotic spindle 4e-050.00111&radic S000001810FLO10YKR102WverifiedLectin-like protein with similarity to Flo1p, thought to beinvolved in flocculation 4e-050.00111 S000006246PUF2YPR042CverifiedMember of the PUF protein family, which is defined by thepresence of Pumilio homology domains thatconfer RNA binding activity; preferentiallybinds mRNAs encoding membrane-associatedproteins 4e-050.00111 S000004730PKR1YMR123WverifiedV-ATPase assembly factor, functions with other V-ATPaseassembly factors in the ER to efficientlyassemble the V-ATPase membrane sector(V0); overproduction confersresistance to Pichia farinosa killer toxin 4e-050.00111 S000001573CUE2YKL090WverifiedProtein of unknown function; has two CUE domains that bindubiquitin, which may facilitate intramolecularmonoubiquitination 4e-050.00111 S000003480SOL4YGR248Wverified6-phosphogluconolactonase with similarity to Sol3p 4e-050.00111&radic S000004829SKY1YMR216CverifiedSR protein kinase (SRPK) involved in regulating proteinsinvolved in mRNA metabolism and cationhomeostasis; similar to human SRPK1 4e-050.00111&radic&radic S000001024GUT1YHL032CverifiedGlycerol kinase, converts glycerol to glycerol-3-phosphate;glucose repression of expression is mediated byAdr1p and Ino2p-Ino4p; derepression ofexpression on non-fermentable carbon sources ismediated by Opi1p and Rsf1p 4e-050.00111 S000000485DUG2YBR281CuncharacterizedProtein involved in alternative pathway of glutathionedegradation; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmand nucleus; YBR281C is not an essential gene 4e-050.00111&radic S000001624SDH3YKL141WverifiedCytochrome b subunit of succinate dehydrogenase (Sdh1p, Sdh2p,Sdh3p, Sdh4p), which couples the oxidation ofsuccinate to the transfer of electrons toubiquinone 4e-050.00111 S000005111SKO1YNL167CverifiedBasic leucine zipper (bZIP) transcription factor of theATF/CREB family, forms a complex with Tup1p andSsn6p to both activate and represstranscription; cytosolic and nuclear proteininvolved in osmotic and oxidative stressresponses 4e-050.00111&radic S000002998RPL30YGL030WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to rat L30 ribosomal protein;involved in pre-rRNA processing in thenucleolus; autoregulates splicing of itstranscript 4e-050.00111 S000000180CDC27YBL084CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition 4e-050.00111&radic S000000840KRE29YER038CverifiedEssential subunit of the Mms21-Smc5-Smc6 complex; protein ofunknown function; required for growth and DNArepair; heterozygous mutant showshaploinsufficiency in K1 killer toxinresistance 4e-050.00111&radic S000001661STE3YKL178CverifiedReceptor for a factor receptor, transcribed in alpha cells andrequired for mating by alpha cells, couples toMAP kinase cascade to mediate pheromoneresponse; ligand bound receptors areendocytosed and recycled to the plasmamembrane; GPCR 4e-050.00111 S000005140YNL196CUncharacterizedSporulation-specific protein with a leucine zipper motif 4e-050.00111 S000007245COX19YLL018C-AverifiedProtein required for cytochrome c oxidase assembly, located inthe cytosol and mitochondrial intermembranespace; putative copper metallochaperone thatdelivers copper to cytochrome c oxidase 4e-050.00111&radic S000005603RTS2YOR077WverifiedBasic zinc-finger protein, similar to human and mouse Kin17proteins which are chromatin-associatedproteins involved in UV response and DNAreplication 4e-050.00111&radic S000000535HIS4YCL030CverifiedMultifunctional enzyme containing phosphoribosyl-ATPpyrophosphatase, phosphoribosyl-AMPcyclohydrolase, and histidinol dehydrogenaseactivities; catalyzes the second, third, ninthand tenth steps in histidine biosynthesis 4e-050.00111 S000004511GSF2YML048WverifiedER localized integral membrane protein that may promotesecretion of certain hexose transporters,including Gal2p; involved in glucose-dependentrepression 4e-050.00111 S000001168YHR126CUncharacterizedHypothetical protein 4e-050.00111 S000000572HMLALPHA2YCL067CverifiedSilenced copy of ALPHA2 at HML; homeobox-domain protein thatassociates with Mcm1p in haploid cells torepress a-specific gene expression andinteracts with a1p in diploid cells to represshaploid-specific gene expression 4e-050.00111 S000003759PAU1YJL223CverifiedPart of 23-member seripauperin multigene family encoded mainlyin subtelomeric regions, active duringalcoholic fermentation, regulated byanaerobiosis, negatively regulated by oxygen,repressed by heme 4e-050.00111&radic S000005506PSF3YOL146WverifiedSubunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), whichis localized to DNA replication origins andimplicated in assembly of the DNA replicationmachinery 4e-050.00111&radic&radic S000006252TAH18YPR048WverifiedProtein with a potential role in DNA replication; displayssynthetic lethal genetic interaction with thepol3-13 allele of POL3, which encodes DNApolymerase delta 4e-050.00111 S000002441LYS14YDR034CverifiedTranscriptional activator involved in regulation of genes ofthe lysine biosynthesis pathway; requires2-aminoadipate semialdehyde as co-inducer 4e-050.00111&radic S000000982ISC10YER180CverifiedProtein required for sporulation, transcript is induced 7.5hours after induction of meiosis, expected toplay significant role in the formation ofreproductive cells 4e-050.00111 S000005573STD1YOR047CverifiedProtein involved in control of glucose-regulated geneexpression; interacts with protein kinaseSnf1p, glucose sensors Snf3p and Rgt2p, andTATA-binding protein Spt15p; acts as aregulator of the transcription factor Rgt1p 4e-050.00111 S000001727IRS4YKR019CverifiedProtein involved in regulation of phosphatidylinositol4,5-bisphosphate concentrations; Irs4p andTax4p bind and activate the phosphatase Inp51p;mutation confers an increase in rDNA silencing 4e-050.00111 S000004515GAL80YML051WverifiedTranscriptional regulator involved in the repression of GALgenes in the absence of galactose; inhibitstranscriptional activation by Gal4p; inhibitionrelieved by Gal3p or Gal1p binding 4e-050.00111&radic S000003934SOF1YLL011WverifiedEssential subunit of the U3 (box C+D) snRNP complex requiredfor 2' O-methylation of pre-RNA; has similarityto the beta subunit of trimeric G-proteins andthe splicing factor Prp4p 4e-050.00111 S000004057PET309YLR067CverifiedSpecific translational activator for the COX1 mRNA, alsoinfluences stability of intron-containing COX1primary transcripts; located in themitochondrial inner membrane 4e-050.00111&radic S000000647YCR051WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene 4e-050.00111&radic S000000478CHK1YBR274WverifiedDNA damage checkpoint effector kinase, mediates mitotic cellcycle arrest via phosphorylation of Pds1p;phosphorylated by checkpoint signal transducerkinase Mec1p; homolog of S. pombe and mammalianChk1 checkpoint kinase 4e-050.00111 S000001081MSC7YHR039CverifiedProtein of unknown function, green fluorescent protein(GFP)-fusion protein localizes to theendoplasmic reticulum; msc7 mutants aredefective in directing meiotic recombinationevents to homologous chromatids 4e-050.00111&radic S000003025YGL057CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria; null mutant exhibits a growth defect on a non-fermentable (respiratory) carbon source; YGL057C is not an essential gene 4e-050.00111 S000002186MPS1YDL028CverifiedDual-specificity kinase required for spindle pole body (SPB)duplication and spindle checkpoint function;substrates include SPB proteins Spc42p,Spc110p, and Spc98p, mitotic exit networkprotein Mob1p, and checkpoint protein Mad1p 4e-050.00111&radic S000003673GLG2YJL137CverifiedSelf-glucosylating initiator of glycogen synthesis, alsoglucosylates n-dodecyl-beta-D-maltoside;similar to mammalian glycogenin 4e-050.00111 S000000046GEM1YAL048CverifiedEvolutionarily-conserved tail-anchored outer mitochondrialmembrane GTPase which regulates mitochondrialmorphology; cells lacking Gem1p containcollapsed, globular, or grape-likemitochondria; not required forpheromone-induced cell death 4e-050.00111&radic S000001193PEX28YHR150WverifiedPeroxisomal integral membrane protein, involved in regulationof peroxisome size and number; geneticinteractions suggest that Pex28p and Pex29p actat steps upstream of those mediated by Pex30p,Pex31p, and Pex32p 4e-050.00111 S000001625MRP8YKL142WverifiedPutative mitochondrial ribosomal protein, has similarity to E.coli ribosomal protein S2 4e-050.00111 S000005651CAT5YOR125CverifiedMitochondrial inner membrane protein directly involved inubiquinone biosynthesis, essential for severalother metabolic pathways including respirationand gluconeogenic gene activation 4e-050.00111&radic&radic S000028518YCL058W-AUncharacterizedProtein of unknown function; identified by homology to Ashbya gossypii 4e-050.00111 S000000883SER3YER081Wverified3-phosphoglycerate dehydrogenase, catalyzes the first step inserine and glycine biosynthesis; isozyme ofSer33p 4e-050.00111&radic&radic S000000653PWP2YCR057CverifiedConserved 90S pre-ribosomal component essential for properendonucleolytic cleavage of the 35 S rRNAprecursor at A0, A1, and A2 sites; containseight WD-repeats; PWP2 deletion leads todefects in cell cycle and bud morphogenesis 4e-050.00111 S000002296ARF2YDL137WverifiedADP-ribosylation factor, GTPase of the Ras superfamily involvedin regulation of coated formation vesicles inintracellular trafficking within the Golgi;functionally interchangeable with Arf1p 4e-050.00111 S000004398RPL31BYLR406CverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl31Ap and has similarity to ratL31 ribosomal protein; associates with thekaryopherin Sxm1p 4e-050.00111 S000006200YPL279CUncharacterizedHypothetical protein 4e-050.00111 S000003184KIP3YGL216WverifiedKinesin-related motor protein involved in mitotic spindlepositioning 4e-050.00111 S000002897SLD5YDR489WverifiedSubunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), whichis localized to DNA replication origins andimplicated in assembly of the DNA replicationmachinery 4e-050.00111 S000000483PAF1YBR279WverifiedRNA polymerase II-associated protein, defines a large complexthat is biochemically and functionally distinctfrom the Srb-Mediator form of Pol II holoenzymeand is required for full expression of a subsetof cell cycle-regulated genes 4e-050.00111&radic&radic S000003381YGR149WUncharacterizedPutative protein of unknown function; predicted to be an integal membrane protein 4e-050.00111 S000000552YCL047CUncharacterizedPutative protein of unknown function 4e-050.00111 S000002984KAP122YGL016WverifiedKaryopherin beta, responsible for import of the Toa1p-Toa2pcomplex into the nucleus; binds to nucleoporinsNup1p and Nup2p; may play a role in regulationof pleiotropic drug resistance 4e-050.00111&radic S000003713RPL17BYJL177WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl17Ap and has similarity to E.coli L22 and rat L17 ribosomal proteins 4e-050.00111 S000005015LAT1YNL071WverifiedDihydrolipoamide acetyltransferase component (E2) of pyruvatedehydrogenase complex, which catalyzes theoxidative decarboxylation of pyruvate toacetyl-CoA 4e-050.00111 S000000814PRE1YER012Wverified20S proteasome beta-type subunit; localizes to the nucleusthroughout the cell cycle 4e-050.00111 S000004585NAB6YML117WuncharacterizedPutative RNA-binding protein, based on computational analysisof large-scale protein-protein interaction data 4e-050.00111&radic S000004295ACO1YLR304CverifiedAconitase, required for the tricarboxylic acid (TCA) cycle andalso independently required for mitochondrialgenome maintenance; component of themitochondrial nucleoid; mutation leads toglutamate auxotrophy 4e-050.00111 S000000534BIK1YCL029CverifiedMicrotubule-associated protein, component of the interfacebetween microtubules and kinetochore, involvedin sister chromatid separation; essential inpolyploid cells but not in haploid or diploidcells; ortholog of mammalian CLIP-170 4e-050.00111 S000001806UBP11YKR098CverifiedUbiquitin-specific protease that cleaves ubiquitin fromubiquitinated proteins 4e-050.00111 S000002878UGO1YDR470CverifiedProtein of unknown function; outer membrane component of themitochondrial fusion machinery; Ugo1p binddirectly to Fzo1p and Mgm1p and thereby linkthese two GTPases during mitochondrial fusion 4e-050.00111 S000001855ACT1YFL039CverifiedActin, structural protein involved in cell polarization,endocytosis, and other cytoskeletal functions 4e-050.00111 S000028532YBR072C-AUncharacterizedIdentified by fungal homology and RT-PCR 4e-050.00111 S000003220HFM1YGL251CverifiedMeiosis specific DNA helicase involved in the conversion ofdouble-stranded breaks to later recombinationintermediates and in crossover control;catalyzes the unwinding of Holliday junctions;has ssDNA and dsDNA stimulated ATPase activity 4e-050.00111 S000000357RIB7YBR153WverifiedDiaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzesthe second step of the riboflavin biosynthesispathway 4e-050.00111 S000005794YOR268CUncharacterizedPutative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene 4e-050.00111 S000003238PRP18YGR006WverifiedSplicing factor involved in the positioning of the 3' splicesite during the second catalytic step ofsplicing, recruited to the spliceosome by Slu7p 4e-050.00111 S000028523YJR005C-AUncharacterizedPutative protein of unknown function, originally identified as a syntenic homolog of an Ashbya gossypii gene 4e-050.00111 S000005211PIK1YNL267WverifiedPhosphatidylinositol 4-kinase; catalyzes first step in thebiosynthesis ofphosphatidylinositol-4,5-biphosphate; maycontrol cytokineses through the actincytoskeleton 4e-050.00111 S000001025RPL8AYHL033CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Bp and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 4e-050.00111 S000006357YPR153WUncharacterizedHypothetical protein 4e-050.00111 S000004141PCD1YLR151CverifiedPeroxisomal nudix pyrophosphatase with specificity for coenzymeA and CoA derivatives, may function to removepotentially toxic oxidized CoA disulfide fromperoxisomes to maintain the capacity forbeta-oxidation of fatty acids 4e-050.00111 S000004007MEU1YLR017WverifiedMethylthioadenosine phosphorylase (MTAP), catalyzes the initialstep in the methionine salvage pathway; affectspolyamine biosynthesis through regulation ofornithine decarboxylase (Spe1p) activity;regulates ADH2 gene expression 4e-050.00111&radic S000003473YAP1802YGR241CverifiedProtein involved in clathrin cage assembly; binds Pan1p andclathrin; homologous to Yap1801p, member of theAP180 protein family 4e-050.00111 S000003543CTK2YJL006CverifiedBeta subunit of C-terminal domain kinase I (CTDK-I), whichphosphorylates the C-terminal repeated domainof the RNA polymerase II large subunit (Rpo21p)to affect both transcription and pre-mRNA 3'end processing; has similarity to cyclins 4e-050.00111&radic S000006434LSM3YLR438C-AverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 4e-050.00111&radic S000005542ERP4YOR016CverifiedProtein with similarity to Emp24p and Erv25p, member of the p24family involved in ER to Golgi transport 4e-050.00111 S000004072SRL2YLR082CverifiedProtein of unknown function; overexpression suppresses thelethality caused by a rad53 null mutation 4e-050.00111&radic S000001196SPO16YHR153CverifiedProtein of unknown function, required for spore formation 4e-050.00111 S000028538YDR169C-AUncharacterizedIdentified by fungal homology and RT-PCR 4e-050.00111 S000005424CRT10YOL063CverifiedProtein involved in transcriptional regulation of RNR2 andRNR3; expression of the gene is induced by DNAdamage and null mutations confer increasedresistance to hydroxyurea; N-terminal regionhas a leucine repeat and a WD40 repeat 4e-050.00111&radic S000002930SPS2YDR522CverifiedProtein expressed during sporulation, redundant with Sps22p fororganization of the beta-glucan layer of thespore wall; S. pombe ortholog is a spore wallcomponent 4e-050.00111 S000005657YOR131CUncharacterizedHypothetical protein 4e-050.00111&radic S000000058GDH3YAL062WverifiedNADP(+)-dependent glutamate dehydrogenase, synthesizesglutamate from ammonia and alpha-ketoglutarate;rate of alpha-ketoglutarate utilization differsfrom Gdh1p; expression regulated by nitrogenand carbon sources 4e-050.00111&radic&radic S000000323MUD1YBR119WverifiedU1 snRNP A protein, homolog of human U1-A; involved in nuclearmRNA splicing 4e-050.00111&radic&radic&radic S000001450STS1YIR011CverifiedProtein that interacts with the karyopherin Srp1p; may have arole with Srp1p in ubiquitin-mediated proteindegradation 4e-050.00111&radic S000001591SLD2YKL108WverifiedProtein required for DNA replication, phosphorylated in S phaseby S-phase cyclin-dependent kinases (Cdks),phosphorylation is essential for DNAreplication and for complex formation withDpb11p; potential Cdc28p substrate 4e-050.00111&radic S000004089ICT1YLR099CverifiedProtein of unknown function, null mutation leads to an increasein sensitivity to Calcofluor white; expressionof the gene is induced in the presence ofisooctane 4e-050.00111 S000001298CST6YIL036WverifiedBasic leucine zipper (bZIP) transcription factor of theATF/CREB family, activates transcription ofgenes involved in utilization of non-optimalcarbon sources; involved in telomeremaintenance 4e-050.00111&radic S000001276MNT3YIL014WverifiedAlpha-1,3-mannosyltransferase, adds the fourth and fifthalpha-1,3-linked mannose residues to O-linkedglycans during protein O-glycosylation 4e-050.00111 S000005711GSP2YOR185CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; interacts withKap121p, Kap123p and Pdr6p (karyophilin betas);Gsp1p homolog that is not required forviability 4e-050.00111&radic S000002819DFM1YDR411CuncharacterizedER localized derlin-like family member involved in ER stressand homeostasis; not involved in ERAD orsubstrate retrotranslocation; interacts withCDC48; contains four transmembrane domains andtwo SHP boxes 4e-050.00111 S000004195HMX1YLR205CverifiedER localized, heme-binding peroxidase involved in thedegradation of heme; does not exhibit hemeoxygenase activity despite similarity to hemeoxygenases; expression regulated by AFT1 4e-050.00111 S000028632YGL007C-AUncharacterizedIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 4e-050.00111 S000001064YHR022CUncharacterizedPutative protein of unknown function; YHR022C is not an essential gene. 4e-050.00111&radic S000003639GSM1YJL103CuncharacterizedPutative zinc cluster protein of unknown function; proposed tobe involved in the regulation of energymetabolism, based on patterns of expression andsequence analysis 4e-050.00111 S000000392NTC20YBR188CverifiedMember of a complex, including Prp19p, that binds to thespliceosome; required for pre-mRNA splicing 4e-050.00111&radic&radic S000005241MON2YNL297CverifiedPeripheral membrane protein with a role in endocytosis andvacuole integrity, interacts with Arl1p andlocalizes to the endosome; member of the Sec7pfamily of proteins 4e-050.00111 S000002398ADY3YDL239CverifiedProtein required for spore wall formation, thought to mediateassembly of a Don1p-containing structure at theleading edge of the prospore membrane viainteraction with spindle pole body components;potentially phosphorylated by Cdc28p 4e-050.00111 S000005191YNL247WUncharacterizedProtein required for cell viability 4e-050.00111 S000002248RAM1YDL090CverifiedBeta subunit of the CAAX farnesyltransferase (FTase) thatprenylates the a-factor mating pheromone andRas proteins; required for the membranelocalization of Ras proteins and a-factor;homolog of the mammalian FTase beta subunit 4e-050.00111 S000004004PPR1YLR014CverifiedZinc finger transcription factor containing a Zn(2)-Cys(6)binuclear cluster domain, positively regulatestranscription of genes involved in uracilbiosynthesis; activity may be modulated byinteraction with Tup1p 4e-050.00111 S000002391OST4YDL232WverifiedSubunit of the oligosaccharyltransferase complex of the ERlumen, which catalyzes proteinasparagine-linked glycosylation; type Imembrane protein required for incorporation ofOst3p or Ost6p into the OST complex 4e-050.00111 S000001703FRE2YKL220CverifiedFerric reductase and cupric reductase, reducessiderophore-bound iron and oxidized copperprior to uptake by transporters; expressioninduced by low iron levels but not by lowcopper levels 4e-050.00111 S000002762TRP4YDR354WverifiedAnthranilate phosphoribosyl transferase of the tryptophanbiosynthetic pathway, catalyzes thephosphoribosylation of anthranilate, subject tothe general control system of amino acidbiosynthesis 4e-050.00111&radic S000001008DUR3YHL016CverifiedPlasma membrane urea transporter, expression is highlysensitive to nitrogen catabolite repression andinduced by allophanate, the last intermediateof the allantoin degradative pathway 4e-050.00111 S000001022ECM29YHL030WverifiedMajor component of the proteasome; tethers the proteasome coreparticle to the regulatory particle, andenhances the stability of the proteasome 4e-050.00111&radic S000004262YCS4YLR272CverifiedNon-SMC subunit of the condensin complex(Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required forestablishment and maintenance of chromosomecondensation, chromosome segregation, chromatinbinding of condensin and silencing at themating type locus 4e-050.00111&radic S000001945YMR31YFR049WverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to human mitochondrial ribosomalprotein MRP-S36 4e-050.00111 S000004831TRS130YMR218CverifiedOne of 10 subunits of the transport protein particle (TRAPP)complex of the cis-Golgi which mediates vesicledocking and fusion; involved in ER to Golgimembrane traffic; mutation activatestranscription of OCH1 4e-050.00111 S000087203YCL048W-AUncharacterizedPutative protein of unknown function 4e-050.00111 S000003615PRY1YJL079CverifiedProtein of unknown function, has similarity to Pry2p and Pry3pand to the plant PR-1 class of pathogen relatedproteins 4e-050.00111 S000001891MSH4YFL003CverifiedProtein involved in meiotic recombination, required for normallevels of crossing over, colocalizes with Zip2pto discrete foci on meiotic chromosomes, hashomology to bacterial MutS protein 4e-050.00111&radic S000005068NAF1YNL124WverifiedProtein required for the assembly of box H/ACA snoRNPs and forpre-rRNA processing, forms a complex with Shq1pand interacts with H/ACA snoRNP componentsNhp2p and Cbf5p; has similarity to Gar1p andother RNA-binding proteins 4e-050.00111&radic S000004206CPR6YLR216CverifiedPeptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzesthe cis-trans isomerization of peptide bondsN-terminal to proline residues; binds to Hsp82pand contributes to chaperone activity 4e-050.00111 S000002153YBL113CUncharacterizedHelicase-like protein encoded within the telomeric Y' element 4e-050.00111 S000002733YCG1YDR325WverifiedNon-SMC subunit of the condensin complex(Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required forestablishment and maintenance of chromosomecondensation, chromosome segregation and forchromatin binding of the condensin complex 4e-050.00111&radic S000006132NIP7YPL211WverifiedNucleolar protein required for 60S ribosome subunit biogenesis,constituent of 66S pre-ribosomal particles;physically interacts with Nop8p and the exosomesubunit Rrp43p 4e-050.00111&radic S000005722LIP5YOR196CverifiedProtein involved in biosynthesis of the coenzyme lipoic acid,has similarity to E. coli lipoic acid synthase 4e-050.00111 S000001006YLF2YHL014CverifiedProtein of unknown function, has weak similarity to E. coliGTP-binding protein gtp1; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 4e-050.00109&radic S000003226MNT2YGL257CverifiedMannosyltransferase involved in adding the 4th and 5th mannoseresidues of O-linked glycans 4e-050.00109 S000004149YLR159WUncharacterizedNote: YLR156W, YLR159W, and YLR161W are identical. 4e-050.00109&radic S000000773YEL047CVerifiedFumurate ReDuctase Soluble 4e-050.00109&radic S000001337RPN2YIL075CverifiedSubunit of the 26S proteasome, substrate of theN-acetyltransferase Nat1p 4e-050.00109&radic S000006205CIT3YPR001WverifiedCitrate synthase, catalyzes the condensation of acetyl coenzymeA and oxaloacetate to form citrate,mitochondrial isozyme involved in the TCA cycle 4e-050.00109 S000005417GPM3YOL056WverifiedHomolog of Gpm1p phosphoglycerate mutase, which converts3-phosphoglycerate to 2-phosphoglycerate inglycolysis; may be non-functional derivative ofa gene duplication event 4e-050.00109 S000003646GZF3YJL110CverifiedGATA zinc finger protein and Dal80p homolog that negativelyregulates nitrogen catabolic gene expression bycompeting with Gat1p for GATA site binding;function requires a repressive carbon source;dimerizes with Dal80p and binds to Tor1p 4e-050.00109&radic S000007606YFR012W-AUncharacterizedPutative protein of unknown function; identified by homology 4e-050.00109 S000006270UBA3YPR066WverifiedProtein that acts together with Ula1p to activate Rub1p beforeits conjugation to proteins (neddylation),which may play a role in protein degradation;GFP-fusion protein localizes to the cytoplasmin a punctate pattern 4e-050.00109 S000002427YDR020CUncharacterizedPredicted protein shares weak similarity with uridine kinases and with phosphoribokinases; null exhibits no apparent phenotype 4e-050.00109&radic S000003159COX13YGL191WverifiedSubunit VIa of cytochrome c oxidase, which is the terminalmember of the mitochondrial inner membraneelectron transport chain; not essential forcytochrome c oxidase activity but may modulateactivity in response to ATP 4e-050.00109 S000000971RPH1YER169WverifiedTranscriptional repressor of PHR1, which is a photolyaseinduced by DNA damage; binds to AG(4) (C(4)T)sequence upstream of PHR1; Rph1pphosphorylation during DNA damage is undercontrol of the MEC1-RAD53 pathway 4e-050.00109&radic S000004898NGL2YMR285CverifiedProtein involved in 5.8S rRNA processing; Ccr4p-like RNaserequired for correct 3'-end formation of 5.8SrRNA at site E; similar to Ngl1p and Ngl3p 4e-050.00109&radic S000004961PUB1YNL016WverifiedPoly(A)+ RNA-binding protein, abundant mRNP-component proteinthat binds mRNA and is required for stabilityof a number of mRNAs; not reported to associatewith polyribosomes 4e-050.00109 S000028561YLR154C-GUncharacterizedPutative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF is contained within RDN25-2 and RDN37-2 4e-050.00109 S000001584HSL1YKL101WverifiedNim1p-related protein kinase that regulates the morphogenesisand septin checkpoints; associates with theassembled septin filament; required along withHsl7p for bud neck recruitment,phosphorylation, and degradation of Swe1p 4e-050.00109 S000002664CTA1YDR256CverifiedCatalase A, breaks down hydrogen peroxide in the peroxisomalmatrix formed by acyl-CoA oxidase (Pox1p)during fatty acid beta-oxidation 4e-050.00109 S000005135DUG3YNL191WuncharacterizedProtein involved in alternative pathway of glutathionedegradation 4e-050.00109 S000004044OSW2YLR054CuncharacterizedProtein of unknown function proposed to be involved in theassembly of the spore wall 4e-050.00109&radic S000004884URA10YMR271CverifiedOne of two orotate phosphoribosyltransferase isozymes (see alsoURA5) that catalyze the fifth enzymatic step inthe de novo biosynthesis of pyrimidines,converting orotate into orotidine-5'-phosphate 4e-050.00109 S000005052YNL108CUncharacterizedPutative protein of unknown function with similarity to TFC7 and phosphotranfer enzymes in prokaryotes; a deletion mutant shows alterations in glucose metabolism; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 4e-050.00109&radic S000003932COX17YLL009CverifiedCopper metallochaperone that transfers copper to Sco1p andCox11p for eventual delivery to cytochrome coxidase 4e-050.00109&radic S000005401NOP12YOL041CverifiedNucleolar protein, required for pre-25S rRNA processing;contains an RNA recognition motif (RRM) and hassimilarity to Nop13p, Nsr1p, and putativeorthologs in Drosophila and S. pombe 4e-050.00109 S000000284SEC18YBR080CverifiedATPase required for the release of Sec17p during the 'priming'step in homotypic vacuole fusion and for ER toGolgi transport; homolog of the mammalian NSF 4e-050.00109 S000000373SSE2YBR169CverifiedMember of the heat shock protein 70 (HSP70) family; may beinvolved in protein folding; localized to thecytoplasm; highly homologous to the heat shockprotein Sse1p 4e-050.00109 S000006173YAH1YPL252CverifiedFerredoxin of the mitochondrial matrix required for formationof cellular iron-sulfur proteins; involved inheme A biosynthesis; homologous to humanadrenodoxin 4e-050.00109 S000002279YDL121CUncharacterizedHypothetical protein 4e-050.00109 S000000896PUP3YER094CverifiedBeta subunit of the 20S proteasome involved inubiquitin-dependent catabolism; human homologis subunit C10 4e-050.00109 S000001195SPO12YHR152WverifiedNucleolar protein of unknown function, positive regulator ofexit from mitosis; involved in regulating therelease of Cdc14p from the nucleolus in earlyanaphase; proposed to play similar role inmeiosis 4e-050.00109&radic S000007246TMA7YLR262C-AverifiedProtein of unknown that associates with ribosomes; null mutantexhibits translation defects, alteredpolyribosome profiles, and resistance to thetranslation inhibitor anisomcyin 4e-050.00109&radic S000001957SAE3YHR079C-AverifiedMeiosis specific protein involved in DMC1-dependent meioticrecombination, forms heterodimer with Mei5p;proposed to be an assembly factor for Dmc1p 4e-050.00109&radic S000005887PDE2YOR360CverifiedHigh-affinity cyclic AMP phosphodiesterase, component of thecAMP-dependent protein kinase signaling system,protects the cell from extracellular cAMP,contains readthrough motif surroundingtermination codon 4e-050.00109&radic S000002187ARP2YDL029WverifiedEssential component of the Arp2/3 complex, which is a highlyconserved actin nucleation center required forthe motility and integrity of actin patches;involved in endocytosis and membrane growth andpolarity 4e-050.00109 S000002843PPM1YDR435CverifiedCarboxyl methyl transferase, methylates the C terminus of theprotein phosphatase 2A catalytic subunit(Pph21p or Pph22p), which is important forcomplex formation with regulatory subunits 4e-050.00109 S000005141WHI3YNL197CverifiedRNA binding protein that sequesters CLN3 mRNA in cytoplasmicfoci; cytoplasmic retention factor for Cdc28pand associated cyclins; regulates cell fate anddose-dependently regulates the critical cellsize required for passage through Start 4e-050.00109&radic&radic S000003985MHT1YLL062CverifiedS-methylmethionine-homocysteine methyltransferase, functionsalong with Sam4p in the conversion ofS-adenosylmethionine (AdoMet) to methionine tocontrol the methionine/AdoMet ratio 4e-050.00109 S000004832ESC1YMR219WverifiedProtein localized to the nuclear periphery, involved intelomeric silencing; interacts with PAD4-domainof Sir4p 4e-050.00109 S000003314TOM20YGR082WverifiedComponent of the TOM (translocase of outer membrane) complexresponsible for recognition and initial importsteps for all mitochondrially directedproteins; acts as a receptor for incomingprecursor proteins 4e-050.00109 S000001828YFL066CUncharacterizedHelicase-like protein encoded within the telomeric Y' element 4e-050.00109 S000003666URA2YJL130CverifiedBifunctional carbamoylphosphate synthetase (CPSase)-aspartatetranscarbamylase (ATCase), catalyzes the firsttwo enzymatic steps in the de novo biosynthesisof pyrimidines; both activities are subject tofeedback inhibition by UTP 4e-050.00109&radic S000003401PUS6YGR169CverifiedPseudouridine synthase responsible for modification ofcytoplasmic and mitochondrial tRNAs at position31; mutation of Asp168 to Ala abolishes enzymeactivity; not essential for viability 4e-050.00109 S000004754TIF34YMR146CverifiedSubunit of the core complex of translation initiation factor3(eIF3), which is essential for translation 4e-050.00109 S000005207YIF1YNL263CverifiedIntegral membrane protein required for the fusion of ER-derivedCOPII transport vesicles with the Golgi;interacts with Yip1p and Yos1p; localizes tothe Golgi, the ER, and COPII vesicles 4e-050.00109 S000000024DRS2YAL026CverifiedAminophospholipid translocase (flippase) that maintainsmembrane lipid asymmetry in post-Golgisecretory vessicles; localizes to thetrans-Golgi network; contributes toclathrin-coated vesicle formation andendocytosis; type 4 P-type ATPase 4e-050.00109 S000002287YDL129WUncharacterizedHypothetical protein 4e-050.00109&radic S000004487YML6YML025CverifiedMitochondrial ribosomal protein of the large subunit, hassimilarity to E. coli L4 ribosomal protein andhuman mitoribosomal MRP-L4 protein; essentialfor viability, unlike most other mitoribosomalproteins 4e-050.00109 S000001277BAR1YIL015WverifiedAspartyl protease secreted into the periplasmic space of matingtype a cells, helps cells find mating partners,cleaves and inactivates alpha factor allowingcells to recover from alpha-factor-induced cellcycle arrest 4e-050.00109 S000004643YET2YMR040WverifiedProtein of unknown function that may interact with ribosomes,based on co-purification experiments; homologof human BAP31 protein 4e-050.00109 S000002953YRF1-1YDR545WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 4e-050.00109 S000001848FMP32YFL046WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 4e-050.00109 S000004110YPS1YLR120CverifiedAspartic protease, attached to the plasma membrane via aglycosylphosphatidylinositol (GPI) anchor 4e-050.00109 S000004296STT4YLR305CverifiedPhosphatidylinositol-4-kinase that functions in the Pkc1pprotein kinase pathway; required for normalvacuole morphology, cell wall integrity, andactin cytoskeleton organization 4e-050.00109&radic S000005090YNL146WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene 4e-050.00109 S000001078BRL1YHR036WverifiedEssential nuclear envelope integral membrane protein identifiedas a suppressor of a cold-sensitive mutant ofCRM1, a karyopherin; homologous to andinteracts with Brr6p, which is a nuclearenvelope protein involved in nuclear export 4e-050.00109 S000003432ELP2YGR200CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;target of Kluyveromyces lactis zymocin 4e-050.00109 S000006188ACM1YPL267WverifiedCell cycle regulated protein of unknown function; associatedwith Cdh1p and may supress theAPC/C[Cdh1]-mediated proteolysis of mitoticcyclins 4e-050.00109 S000003053YGL085WUncharacterizedPutative protein of unknown function, has homology to Staphylococcus aureus nuclease; green fluorescent protein (GFP)-fusion protein localizes to mitochondria 4e-050.00109 S000005125YNL181WUncharacterizedProtein required for cell viability 4e-050.00109 S000000015PSK1YAL017WverifiedOne of two (see also PSK2) PAS domain containing S/T proteinkinases; coordinately regulates proteinsynthesis and carbohydrate metabolism andstorage in response to a unknown metabolitethat reflects nutritional status 4e-050.00109 S000001443DJP1YIR004WverifiedCytosolic J-domain-containing protein, required for peroxisomalprotein import and involved in peroxisomeassembly, homologous to E. coli DnaJ 4e-050.00109 S000000368ARL1YBR164CverifiedSoluble GTPase with a role in regulation of membrane traffic;regulates potassium influx; G protein of theRas superfamily, similar to ADP-ribosylationfactor 4e-050.00109 S000001477GTT1YIR038CverifiedER associated glutathione S-transferase capable ofhomodimerization; expression induced during thediauxic shift and throughout stationary phase;functional overlap with Gtt2p, Grx1p, and Grx2p 4e-050.00109 S000001247MNL1YHR204WverifiedAlpha mannosidase-like protein of the endoplasmic reticulumrequired for degradation of glycoproteins butnot for processing of N-linked oligosaccharides 4e-050.00109&radic S000001814YKR106WUncharacterizedProtein of unconfirmed function; displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins; coding sequence 98% identical to that of YCL073C 4e-050.00109 S000000045SPC72YAL047CverifiedComponent of the cytoplasmic Tub4p (gamma-tubulin) complex,binds spindle pole bodies and links them tomicrotubules; has roles in astral microtubuleformation and stabilization 4e-050.00109 S000003130SUT1YGL162WverifiedTranscription factor of the Zn[II]2Cys6 family involved insterol uptake; involved in induction of hypoxicgene expression 4e-050.00109&radic S000005409GSH2YOL049WverifiedGlutathione synthetase, catalyzes the ATP-dependent synthesisof glutathione (GSH) fromgamma-glutamylcysteine and glycine; induced byoxidative stress and heat shock 4e-050.00109 S000000837EDC2YER035WverifiedRNA-binding protein, activates mRNA decapping directly bybinding to the mRNA substrate and enhancing theactivity of the decapping proteins Dcp1p andDcp2p 4e-050.00109&radic S000006156RVB2YPL235WverifiedEssential protein involved in transcription regulation;component of chromatin remodeling complexes;required for assembly and function of the INO80complex; member of the RUVB-like protein family 4e-050.00109 S000005485TRM13YOL125WuncharacterizedHypothetical protein 4e-050.00109&radic S000007592PAU9YBL108C-AuncharacterizedHypothetical protein identified by homology. See FEBS Letters[2000] 487:31-36. 4e-050.00109 S000002731PEP7YDR323CverifiedMultivalent adaptor protein that facilitates vesicle-mediatedvacuolar protein sorting by ensuringhigh-fidelity vesicle docking and fusion, whichare essential for targeting of vesicles to theendosome; required for vacuole inheritance 4e-050.00109 S000005110BNI5YNL166CverifiedProtein involved in organization of septins at the mother-budneck, may interact directly with the Cdc11pseptin, localizes to bud neck in aseptin-dependent manner 4e-050.00109 S000005572DBP5YOR046CverifiedCytoplasmic ATP-dependent RNA helicase of the DEAD-box familyinvolved in mRNA export from the nucleus 4e-050.00109 S000000471REI1YBR267WverifiedCytoplasmic pre-60S factor; required for the correct recyclingof shuttling factors Alb1, Arx1 and Tif6 at theend of the ribosomal large subunit biogenesis;involved in bud growth in the mitotic signalingnetwork 4e-050.00109 S000003139ROK1YGL171WverifiedATP-dependent RNA helicase of the DEAD box family; required for18S rRNA synthesis 4e-050.00109 S000005596GYP1YOR070CverifiedCis-golgi GTPase-activating protein (GAP) for the Rab familymembers Ypt1p (in vivo) and for Ypt1p, Sec4p,Ypt7p, and Ypt51p (in vitro); involved invesicle docking and fusion 4e-050.00109 S000002535YDR128WUncharacterizedHypothetical protein 4e-050.00109&radic S000004078GAA1YLR088WverifiedSubunit of the GPI (glycosylphosphatidylinositol):proteintransamidase complex, removes the GPI-anchoringsignal and attaches GPI to proteins in the ER 4e-050.00109 S000003699YJL163CUncharacterizedHypothetical protein 4e-050.00109&radic&radic S000000452HIS7YBR248CverifiedImidazole glycerol phosphate synthase (glutamineamidotransferase:cyclase), catalyzes the fifthand sixth steps of histidine biosynthesis andalso produces 5-aminoimidazole-4-carboxamideribotide (AICAR), a purine precursor 4e-050.00109&radic S000002974PMC1YGL006WverifiedVacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ions; prevents growth inhibition by activationof calcineurin in the presence of elevatedconcentrations of calcium; similar to mammalianPMCA1a 4e-050.00109 S000000701PAU3YCR104WverifiedPart of 23-member seripauperin multigene family encoded mainlyin subtelomeric regions, active duringalcoholic fermentation, regulated byanaerobiosis, negatively regulated by oxygen,repressed by heme 4e-050.00109 S000004087HRT3YLR097CuncharacterizedPutative nuclear ubiquitin ligase, based on computationalanalysis of large-scale protein-proteininteraction data; has similarity to F-boxproteins; identified in association withCdc53p, Skp1p and Ubi4 in large-scale studies 4e-050.00109 S000003295SPT4YGR063CverifiedProtein that forms a complex with Spt5p and mediates bothactivation and inhibition of transcriptionelongation, and plays a role in pre-mRNAprocessing; in addition, Spt4p is involved inkinetochore function and gene silencing 4e-050.00109&radic S000002892VPS52YDR484WverifiedComponent of the GARP (Golgi-associated retrograde protein)complex, Vps51p-Vps52p-Vps53p-Vps54p, which isrequired for the recycling of proteins fromendosomes to the late Golgi; involved inlocalization of actin and chitin 4e-050.00109&radic S000001132YNG2YHR090CverifiedSubunit of the NuA4 histone acetyltransferase complex thatacetylates histone H4 and H2A; has similarityto the human tumor suppressor ING1 4e-050.00109&radic&radic S000000989YER187WUncharacterizedHypothetical protein 4e-050.00109 S000001164YHR122WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; may be involved in transcription; YHR122W is an essential gene 4e-050.00109 S000002448RSM10YDR041WverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S10 ribosomal protein;essential for viability, unlike most othermitoribosomal proteins 4e-050.00109&radic&radic S000003508RNH70YGR276Cverified3' exoribonuclease, required for 5S and tRNA-Arg3 maturation 4e-050.00109&radic S000001649TPK3YKL166CverifiedSubunit of cAMP-dependent protein kinase, which contains Tpk1p,Tpk2p, Tpk3p, and regulatory subunit Bcy1p;promotes growth in response to nutrients;mediates induction of apoptosis due toactin-mediated hyperactivation of Ras signaling 4e-050.00109&radic S000028586YOR376W-AUncharacterizedIdentified by fungal homology and RT-PCR 4e-050.00109 S000004681PDS5YMR076CverifiedProtein required for establishment and maintenance of sisterchromatid condensation and cohesion,colocalizes with cohesin on chromosomes in aninterdependent manner, may function as aprotein-protein interaction scaffold 4e-050.00109&radic S000001392ASG1YIL130WuncharacterizedProposed transcriptional activator, member of the Gal4p familyof zinc cluster proteins 4e-050.00109&radic S000001105PAN5YHR063Cverified2-dehydropantoate 2-reductase, part of the pantothenic acidpathway, structurally homologous to E. colipanE 4e-050.00109&radic&radic S000002290CDC53YDL132WverifiedCullin, structural protein of SCF complexes (which also containSkp1p, Cdc34p, and an F-box protein) involvedin ubiquitination; SCF promotes the G1-Stransition by targeting G1 cyclins and theCln-CDK inhibitor Sic1p for degradation 4e-050.00109&radic S000003576NUP192YJL039CverifiedEssential structural subunit of the nuclear pore complex (NPC),localizes to the nuclear periphery of nuclearpores, homologous to human p205 4e-050.00109 S000006101TCO89YPL180WverifiedSubunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complexthat regulates growth in response to nutrientavailability; cooperates with Ssd1p in themaintenance of cellular integrity; deletionstrains are hypersensitive to rapamycin 4e-050.00109 S000005809YOR283WUncharacterizedPutative protein of unknown function with some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene 4e-050.00109&radic S000000171SSA3YBL075CverifiedATPase involved in protein folding and the response to stress;plays a role in SRP-dependent cotranslationalprotein-membrane targeting and translocation;member of the heat shock protein 70 (HSP70)family; localized to the cytoplasm 4e-050.00109 S000003707YJL171CVerifiedGPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein 4e-050.00109 S000000290IST2YBR086CverifiedPlasma membrane protein that may be involved in osmotolerance,localizes to the mother cell in small-buddedcells and to the bud in medium- andlarge-budded cells; mRNA is transported to thebud tip by an actomyosin-driven process 4e-050.00109 S000002435REG1YDR028CverifiedRegulatory subunit of type 1 protein phosphatase Glc7p,involved in negative regulation ofglucose-repressible genes 4e-050.00109 S000007255TIM11YDR322C-AverifiedSubunit e of mitochondrial F1F0-ATPase, which is a large,evolutionarily conserved enzyme complexrequired for ATP synthesis; essential for thedimeric state of ATP synthase 4e-050.00109 S000000178ALG3YBL082CverifiedDolichol-P-Man dependent alpha(1-3) mannosyltransferase,involved in the synthesis of dolichol-linkedoligosaccharide donor for N-linkedglycosylation of proteins 4e-050.00109 S000005732NOC2YOR206WverifiedProtein that forms a nucleolar complex with Mak21p that bindsto 90S and 66S pre-ribosomes, as well as anuclear complex with Noc3p that binds to 66Spre-ribosomes; both complexes mediateintranuclear transport of ribosomal precursors 4e-050.00109&radic&radic S000004644YMR041CUncharacterizedPutative protein of unknown function with similarity to aldo/keto reductases; YMR041C is not an essential gene 4e-050.00109 S000003435YGR203WUncharacterizedPutative protein with sequence similarity to protein tyrosine phosphatases of the CDC25-like phosphatase family; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 4e-050.00109&radic S000002599HST4YDR191WverifiedMember of the Sir2 family of NAD(+)-dependent proteindeacetylases; involved along with Hst3p insilencing at telomeres, cell cycle progression,radiation resistance, genomic stability andshort-chain fatty acid metabolism 4e-050.00109&radic S000005036YNL092WUncharacterizedPutative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene 4e-050.00109 S000002403THI13YDL244WverifiedProtein involved in synthesis of the thiamine precursorhydroxymethylpyrimidine (HMP); member of asubtelomeric gene family including THI5, THI11,THI12, and THI13 4e-050.00109 S000003170ARO8YGL202WverifiedAromatic aminotransferase, expression is regulated by generalcontrol of amino acid biosynthesis 4e-050.00109 S000028845YLR361C-AUncharacterizedPutative protein of unknown function 4e-050.00109 S000002616MSS4YDR208WverifiedPhosphatidylinositol-4-phosphate 5-kinase, involved in actincytoskeleton organization and cellmorphogenesis; multicopy suppressor of stt4mutation 4e-050.00109 S000006187DIM1YPL266WverifiedEssential 18S rRNA dimethylase, responsible for conservedm6(2)Am6(2)A dimethylation in 3'-terminal loopof 18 S rRNA, part of 90S and 40S pre-particlesin nucleolus, involved in pre-ribosomal RNAprocessing 4e-050.00109&radic S000003744NUC1YJL208CverifiedMajor mitochondrial nuclease, has RNAse and DNA endo- andexonucleolytic activities; has a role inmitochondrial recombination 4e-050.00109 S000005160RAP1YNL216WverifiedDNA-binding protein involved in either activation or repressionof transcription, depending on binding sitecontext; also binds telomere sequences andplays a role in telomeric position effect(silencing) and telomere structure 4e-050.00109&radic S000001872FRS2YFL022CverifiedAlpha subunit of cytoplasmic phenylalanyl-tRNA synthetase,forms a tetramer with Frs1p to form activeenzyme; evolutionarily distant frommitochondrial phenylalanyl-tRNA synthetasebased on protein sequence, but substratebinding is similar 4e-050.00109 S000004039YLR049CUncharacterizedHypothetical protein 4e-050.00109 S000003178YPT32YGL210WverifiedGTPase of the Ypt/Rab family, very similar to Ypt31p; involvedin the exocytic pathway; mediates intra-Golgitraffic or the budding of post-Golgi vesiclesfrom the trans-Golgi 4e-050.00109&radic S000000487SSH1YBR283CverifiedSubunit of the Ssh1 translocon complex; Sec61p homolog involvedin co-translational pathway of proteintranslocation; not essential 4e-050.00109 S000000112FUS3YBL016WverifiedMitogen-activated protein kinase involved in mating pheromoneresponse; activated by phosphorylation bySte7p; provides specificity during the matingvs. filamentous growth response byphosphorylating transcriptional and cytoplasmictargets 4e-050.00109&radic&radic S000001206SOL3YHR163Wverified6-phosphogluconolactonase, catalyzes the second step of thepentose phosphate pathway; weak multicopysuppressor of los1-1 mutation; homologous toSol2p and Sol1p 4e-050.00109&radic S000002985ATE1YGL017WverifiedArginyl-tRNA-protein transferase, catalyzes post-translationalconjugation of arginine to the amino termini ofacceptor proteins which are then subject todegradation via the N-end rule pathway 4e-050.00109 S000007266AI5_BETAQ0075uncharacterizedProtein of unknown function, encoded within an intron of themitochondrial COX1 gene; translationalinitiation codon is predicted to be ATA ratherthan ATG 4e-050.00109 S000005838HSD1YOR311CverifiedEndoplasmic reticulum (ER)-resident membrane protein,overproduction induces enlargement of ER-likemembrane structures and suppresses atemperature-sensitive sly1 mutation 4e-050.00109 S000001352ICE2YIL090WverifiedIntegral ER membrane protein with type-III transmembranedomains; mutations cause defects in cortical ERmorphology in both the mother and daughtercells 4e-050.00109 S000006384AOS1YPR180WverifiedNuclear protein that acts as a heterodimer with Uba2p toactivate Smt3p (SUMO) before its conjugation toproteins (sumoylation), which may play a rolein protein targeting; essential for viability 4e-050.00109&radic&radic S000001712ECM9YKR004CverifiedNon-essential protein of unknown function 4e-050.00109 S000003375SKN1YGR143WverifiedProtein involved in sphingolipid biosynthesis; type II membraneprotein with similarity to Kre6p 4e-050.00109 S000000142PSY4YBL046WverifiedPutative regulatory subunit of an evolutionarily conservedprotein phosphatase complex containing thecatalytic subunit Pph3p and a third subunitPsy2p; required for cisplatin resistance;GFP-fusion protein localizes to the nucleus 4e-050.00109&radic S000001233ERG9YHR190WverifiedFarnesyl-diphosphate farnesyl transferase (squalene synthase),joins two farnesyl pyrophosphate moieties toform squalene in the sterol biosynthesispathway 4e-050.00109 S000001663RPL17AYKL180WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl17Bp and has similarity to E.coli L22 and rat L17 ribosomal proteins;copurifies with the Dam1 complex (aka DASHcomplex) 4e-050.00109 S000000337HSL7YBR133CverifiedProtein arginine N-methyltransferase that exhibits septin andHsl1p-dependent bud neck localization andperiodic Hsl1p-dependent phosphorylation;required along with Hsl1p for bud neckrecruitment, phosphorylation, and degradationof Swe1p 4e-050.00109 S000002955YEL076C-AUncharacterizedHypothetical protein 4e-050.00109 S000003478BRF1YGR246CverifiedTFIIIB B-related factor, one of three subunits of RNApolymerase III transcription initiation factorTFIIIB, binds TFIIIC and TBP and recruits RNApol III to promoters, amino-terminal half ishomologous to TFIIB 4e-050.00109&radic S000000038CLN3YAL040CverifiedG1 cyclin involved in cell cycle progression; activates Cdc28pkinase to promote the G1 to S phase transition;plays a role in regulating transcription of theother G1 cyclins, CLN1 and CLN2; regulated byphosphorylation and proteolysis 4e-050.00109 S000001198YSP1YHR155WuncharacterizedMitochondrial protein with a potential role in promotingmitochondrial fragmentation during programmedcell death in response to high levels ofalpha-factor mating pheromone or the drugamiodarone 4e-050.00109 S000004659BUB2YMR055CverifiedMitotic exit network regulator, forms GTPase-activatingBfa1p-Bub2p complex that binds Tem1p andspindle pole bodies, blocks cell cycleprogression before anaphase in response tospindle and kinetochore damage 4e-050.00109 S000002996SCW11YGL028CverifiedCell wall protein with similarity to glucanases; may play arole in conjugation during mating based on itsregulation by Ste12p 4e-050.00109&radic&radic S000004731YMR124WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene 4e-050.00109 S000003917THI11YJR156CverifiedProtein involved in synthesis of the thiamine precursorhydroxymethylpyrimidine (HMP); member of asubtelomeric gene family including THI5, THI11,THI12, and THI13 4e-050.00109 S000003606YJL070CUncharacterizedPutative protein of unknown function with similarity to AMP deaminases; YJL070C is a non-essential gene 4e-050.00109&radic&radic S000005060DMA2YNL116WverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma1p;homolog of S. pombe Dma1 and H. sapiens Chfr 4e-050.00109 S000005382YOL022CUncharacterizedProtein required for cell viability 4e-050.00109 S000003038RPB9YGL070CverifiedRNA polymerase II subunit B12.6; contacts DNA; mutations affecttranscription start site; involved in telomeremaintenance 4e-050.00109&radic S000005076KRE33YNL132WverifiedEssential protein of unknown function; heterozygous mutantshows haploinsufficiency in K1 killer toxinresistance 4e-050.00109 S000003952YLL029WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL029W is not an essential gene but mutant is defective in spore formation 4e-050.00109 S000001925PTR3YFR029WverifiedComponent of the SPS plasma membrane amino acid sensor system(Ssy1p-Ptr3p-Ssy5p), which senses externalamino acid concentration and transmitsintracellular signals that result in regulationof expression of amino acid permease genes 4e-050.00109 S000002341LYS20YDL182WverifiedHomocitrate synthase isozyme, catalyzes the condensation ofacetyl-CoA and alpha-ketoglutarate to formhomocitrate, which is the first step in thelysine biosynthesis pathway; highly similar tothe other isozyme, Lys21p 4e-050.00109&radic S000003281SCM4YGR049WverifiedPotential regulatory effector of CDC4 function, suppresses atemperature-sensitive allele of CDC4,tripartite protein structure in which a chargedregion separates two uncharged domains, notessential for mitosis or meiosis 4e-050.00109 S000000986YER184CUncharacterizedHypothetical protein 4e-050.00109 S000005557CRS5YOR031WverifiedCopper-binding metallothionein, required for wild-type copperresistance 4e-050.00109 S000001178SPL2YHR136CverifiedProtein with similarity to cyclin-dependent kinase inhibitors,overproduction suppresses a plc1 null mutation;green fluorescent protein (GFP)-fusion proteinlocalizes to the cytoplasm in a punctatepattern 4e-050.00109 S000002400YDL241WUncharacterizedHypothetical protein 4e-050.00109 S000001739SPO14YKR031CverifiedPhospholipase D, catalyzes the hydrolysis ofphosphatidylcholine, producing choline andphosphatidic acid; involved inSec14p-independent secretion; required formeiosis and spore formation; differentlyregulated in secretion and meiosis 4e-050.00109 S000001672HYM1YKL189WverifiedComponent of the RAM signaling network that is involved inregulation of Ace2p activity and cellularmorphogenesis, interacts with Kic1p and Sog2p,localizes to sites of polarized growth duringbudding and during the mating response 4e-050.00109&radic S000001751YKR043CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 4e-050.00109&radic S000002799YDR391CUncharacterizedHypothetical protein 4e-050.00109&radic S000004107CLF1YLR117CverifiedEssential splicesome assembly factor; contains multipletetratricopeptide repeat (TPR) protein-bindingmotifs and interacts specifically with manyspliceosome components, may serve as a scaffoldduring splicesome assembly 4e-050.00109&radic S000005370RCL1YOL010WverifiedRNA terminal phosphate cyclase-like protein involved in rRNAprocessing at sites A0, A1, and A2; does notpossess detectable RNA cyclase activity 4e-050.00109&radic S000002723IPK1YDR315CverifiedInositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear proteinrequired for synthesis of1,2,3,4,5,6-hexakisphosphate (phytate), whichis integral to cell function; has 2 motifsconserved in other fungi; ipk1 gle1 doublemutant is inviable 4e-050.00109 S000002785ATP17YDR377WverifiedSubunit f of the F0 sector of mitochondrial F1F0 ATP synthase,which is a large, evolutionarily conservedenzyme complex required for ATP synthesis 4e-050.00109 S000005929CHL1YPL008WverifiedConserved nuclear protein required to establishsister-chromatid pairing during S-phase,probable DNA helicase with similarity to humanBACH1, which associates with tumor suppressorBRCA1; associates with acetyltransferase Ctf7p 4e-050.00109&radic S000001638RSM22YKL155CverifiedMitochondrial ribosomal protein of the small subunit 4e-050.00109&radic S000000170AAR2YBL074CverifiedComponent of the U5 snRNP, required for splicing of U3precursors; originally described as a splicingfactor specifically required for splicingpre-mRNA of the MATa1 cistron 4e-050.00109&radic S000006074RAD53YPL153CverifiedProtein kinase, required for cell-cycle arrest in response toDNA damage; activated by transautophosphorylation when interacting withhyperphosphorylated Rad9p; also interacts withARS1 and plays a role in initiation of DNAreplication 4e-050.00109&radic S000000780RPL12AYEL054CverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl12Bp; rpl12a rpl12b doublemutant exhibits slow growth and slowtranslation; has similarity to E. coli L11 andrat L12 ribosomal proteins 4e-050.00109 S000004074RAX2YLR084CverifiedN-glycosylated protein involved in the maintenance of bud siteselection during bipolar budding; localizationrequires Rax1p 4e-050.00109 S000001043OSH7YHR001WverifiedMember of an oxysterol-binding protein family with sevenmembers in S. cerevisiae; family members haveoverlapping, redundant functions in sterolmetabolism and collectively perform a functionessential for viability 4e-050.00109 S000001421BNR1YIL159WverifiedFormin, nucleates the formation of linear actin filaments,involved in cell processes such as budding andmitotic spindle orientation which require theformation of polarized actin cables,functionally redundant with BNI1 4e-050.00109 S000004489YOX1YML027WverifiedHomeodomain-containing transcriptional repressor, binds toMcm1p and to early cell cycle boxes (ECBs) inthe promoters of cell cycle-regulated genesexpressed in M/G1 phase; expression is cellcycle-regulated; potential Cdc28p substrate 4e-050.00109&radic S000003326VAS1YGR094WverifiedMitochondrial and cytoplasmic valyl-tRNA synthetase 4e-050.00109&radic S000001679YKT6YKL196CverifiedVesicle membrane protein (v-SNARE) with acyltransferaseactivity; involved in trafficking to and withinthe Golgi, endocytic trafficking to thevacuole, and vacuolar fusion; membranelocalization due to prenylation at thecarboxy-terminus 4e-050.00109 S000000319LYS2YBR115CverifiedAlpha aminoadipate reductase, catalyzes the reduction ofalpha-aminoadipate to alpha-aminoadipate6-semialdehyde, which is the fifth step inbiosynthesis of lysine; activation requiresposttranslational phosphopantetheinylation byLys5p 4e-050.00109 S000000745MMS21YEL019CverifiedSUMO ligase involved in chromosomal organization and DNArepair; essential subunit of theMms21-Smc5-Smc6 complex; mutants are sensitiveto methyl methanesulfonate and show increasedspontaneous mutation and mitotic recombination 4e-050.00109&radic&radic S000004177SKG3YLR187WuncharacterizedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cellperiphery, cytoplasm, bud, and bud neck;potential Cdc28p substrate; similar to Caf120pand Skg4p 4e-050.00109 S000001106SSZ1YHR064CverifiedHsp70 protein that interacts with Zuo1p (a DnaJ homolog) toform a ribosome-associated complex that bindsthe ribosome via the Zuo1p subunit; alsoinvolved in pleiotropic drug resistance viasequential activation of PDR1 and PDR5; bindsATP 4e-050.00109 S000004267YSH1YLR277CverifiedPutative endonuclease, subunit of the mRNA cleavage andpolyadenylation specificity complex requiredfor 3' processing of mRNAs 4e-050.00109&radic&radic S000001285YKE4YIL023CverifiedZinc transporter; localizes to the ER; null mutant is sensitiveto calcofluor white, leads to zinc accumulationin cytosol; ortholog of the mouse KE4 andmember of the ZIP (ZRT, IRT-like Protein)family 4e-050.00109 S000007616YKL106C-AUncharacterizedPutative protein of unknown function; identified by homology to uncharacterized proteins in other fungi 4e-050.00109 S000005602SKI7YOR076CverifiedAntiviral adaptor protein that mediates interactions via itsN-terminus between the exosome and Ski complex(Ski2p, Ski3p, Ski8p) which operate in the3'-to-5' mRNA-decay pathway; cytoplasmicprotein required for degrading nonstop mRNAs 4e-050.00109 S000002176ERP3YDL018CverifiedProtein with similarity to Emp24p and Erv25p, member of the p24family involved in ER to Golgi transport 4e-050.00109 S000003293ADE6YGR061CverifiedFormylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzesa step in the 'de novo' purine nucleotidebiosynthetic pathway 4e-050.00109 S000005806FSH3YOR280CverifiedSerine hydrolase; sequence is similar to Fsh1p and Fsh2p 4e-050.00109 S000001802RPL40BYKR094CverifiedFusion protein, identical to Rpl40Ap, that is cleaved to yieldubiquitin and a ribosomal protein of the large(60S) ribosomal subunit with similarity to ratL40; ubiquitin may facilitate assembly of theribosomal protein into ribosomes 4e-050.00109 S000001776BET3YKR068CverifiedHydrophilic protein that acts in conjunction with SNAREproteins in targeting and fusion of ER to Golgitransport vesicles; component of the TRAPP(transport protein particle) complex 4e-050.00109 S000004589NDI1YML120CverifiedNADH:ubiquinone oxidoreductase, transfers electrons from NADHto ubiquinone in the respiratory chain but doesnot pump protons, in contrast to the highereukaryotic multisubunit respiratory complex I;homolog of human AMID 4e-050.00109 S000007611YJL062W-AUncharacterizedPutative protein of unknown function, identified based on comparison to related yeast species; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria 4e-050.00109 S000003374BTN2YGR142Wverifiedv-SNARE binding protein that facilitates specific proteinretrieval from a late endosome to the Golgi;modulates arginine uptake, possible role inmediating pH homeostasis between the vacuoleand plasma membrane H(+)-ATPase 4e-050.00109 S000002663RMD5YDR255CverifiedCytosolic protein required for sporulation; also required forthe ubiquitination of the gluconeogeneticenzyme fructose-1,6-bisphosphatase, which isdegraded rapidly after the switch fromgluconeogenesis to glycolysis 4e-050.00109 S000028576YMR230W-AUncharacterizedPutative protein of unknown function 4e-050.00109 S000001494CCE1YKL011CverifiedMitochondrial cruciform cutting endonuclease, cleaves Hollidayjunctions formed during recombination ofmitochondrial DNA 4e-050.00109&radic&radic S000005514ZPS1YOL154WverifiedPutative GPI-anchored protein; transcription is induced underlow-zinc conditions, as mediated by the Zap1ptranscription factor, and at alkaline pH 4e-050.00109 S000001613SHE2YKL130CverifiedRNA-binding protein that binds specific mRNAs and interactswith She3p; part of the mRNA localizationmachinery that restricts accumulation ofcertain proteins to the bud 4e-050.00109&radic S000005251MCK1YNL307CverifiedProtein serine/threonine/tyrosine (dual-specificity) kinaseinvolved in control of chromosome segregationand in regulating entry into meiosis; relatedto mammalian glycogen synthase kinases of theGSK-3 family 4e-050.00109&radic S000005181YTP1YNL237WverifiedProbable type-III integral membrane protein of unknownfunction, has regions of similarity tomitochondrial electron transport proteins 4e-050.00109 S000001858RPO41YFL036WverifiedMitochondrial RNA polymerase; single subunit enzyme similar tothose of T3 and T7 bacteriophages; requires aspecificity subunit encoded by MTF1 forpromoter recognition 4e-050.00109 S000004427TSR2YLR435WverifiedProtein with a potential role in pre-rRNA processing 4e-050.00109&radic&radic&radic S000002734YSP2YDR326CverifiedProtein involved in programmed cell death; mutant showsresistance to cell death induced by amiodaroneor intracellular acidification 4e-050.00109 S000005662IDH2YOR136WverifiedSubunit of mitochondrial NAD(+)-dependent isocitratedehydrogenase, which catalyzes the oxidation ofisocitrate to alpha-ketoglutarate in the TCAcycle 4e-050.00109 S000005041PHO23YNL097CverifiedProbable component of the Rpd3 histone deacetylase complex,involved in transcriptional regulation of PHO5;C-terminus has similarity to human candidatetumor suppressor p33(ING1) 4e-050.00109&radic S000003966FPS1YLL043WverifiedPlasma membrane glycerol channel, member of the major intrinsicprotein (MIP) family of channel proteins;involved in efflux of glycerol and in uptake ofthe trivalent metalloids arsenite andantimonite 4e-050.00109 S000007526YOR396WUncharacterizedHypothetical protein 4e-050.00109 S000005802CAF20YOR276WverifiedPhosphoprotein of the mRNA cap-binding complex involved intranslational control, repressor ofcap-dependent translation initiation, competeswith eIF4G for binding to eIF4E 4e-050.00109 S000002737PEX3YDR329CverifiedPeroxisomal membrane protein (PMP) required to recruit Pex19pchaperone to peroxisomes; plays selective,essential, direct role in PMP import as adocking factor for Pex19p 4e-050.00109 S000002402AAD4YDL243CverifiedPutative aryl-alcohol dehydrogenase with similarity to P.chrysosporium aryl-alcohol dehydrogenase,involved in the oxidative stress response 4e-050.00109 S000002207KNH1YDL049CverifiedProtein with similarity to Kre9p, which is involved in cellwall beta 1,6-glucan synthesis; overproductionsuppresses growth defects of a kre9 null mutant 4e-050.00109&radic S000002704MHR1YDR296WverifiedProtein involved in homologous recombination in mitochondriaand in transcription regulation in nucleus;binds to activation domains of acidicactivators; required forrecombination-dependent mtDNA partitioning 4e-050.00109 S000003436ADE3YGR204WverifiedCytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase,involved in single carbon metabolism andrequired for biosynthesis of purines,thymidylate, methionine, and histidine 4e-050.00109&radic S000004155PUS5YLR165CverifiedPseudouridine synthase, catalyzes only the formation ofpseudouridine (Psi)-2819 in mitochondrial 21SrRNA; not essential for viability 4e-050.00109&radic S000004835FSH2YMR222CverifiedSerine hydrolase that localizes to the cytoplasm; sequence issimilar to Fsh1p and Fsh3p 4e-050.00109 S000001200REC104YHR157WverifiedProtein involved in early stages of meiotic recombination;required for meiotic crossing over; forms acomplex with Rec102p and Spo11p necessaryduring the initiation of recombination 4e-050.00109 S000004203CRR1YLR213CverifiedPutative glycoside hydrolase of the spore wall envelope;required for normal spore wall assembly,possibly for cross-linking between the glucanand chitosan layers; expressed duringsporulation