Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0016874 "ligase activity"

GO branch: MF
Number of currently annotated genes: 121

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest Huh:cytoplasmLum:MethotrexateLum:TamoxifenLum:Valproic AcidTAP:69Lum:TroglitazoneLum:5-FUKumar:cyto.Lum:Nalidixic acidHarbison:MGA1_H2O2Hi S000002290CDC53YDL132WverifiedCullin, structural protein of SCF complexes (which also containSkp1p, Cdc34p, and an F-box protein) involvedin ubiquitination; SCF promotes the G1-Stransition by targeting G1 cyclins and theCln-CDK inhibitor Sic1p for degradation &radic0.588890.95765&radic&radic S000000172ILS1YBL076CverifiedCytoplasmic isoleucine-tRNA synthetase, target of theG1-specific inhibitor reveromycin A &radic0.576840.95765&radic&radic&radic&radic&radic&radic&radic&radic S000001061DED81YHR019CverifiedCytosolic asparaginyl-tRNA synthetase, required for proteinsynthesis, catalyzes the specific attachment ofasparagine to its cognate tRNA &radic0.573360.95765&radic&radic&radic&radic S000004117APC2YLR127CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; similar tocullin Cdc53p &radic0.610050.95765&radic&radic&radic&radic&radic&radic S000000927RSP5YER125WverifiedUbiquitin-protein ligase involved in ubiquitin-mediated proteindegradation; plays a role in heat shock element(HSE)-mediated gene expression andmultivesicular body sorting; contains a hect(homologous to E6-AP carboxyl terminus) domain &radic0.577290.95765&radic S000001062YHR020WUncharacterizedPutative protein of unknown function, has similarity to proline-tRNA ligase; YHR020W is an essential gene &radic0.515630.95304&radic&radic&radic S000003030PYC1YGL062WverifiedPyruvate carboxylase isoform, cytoplasmic enzyme that convertspyruvate to oxaloacetate; highly similar toisoform Pyc2p but differentially regulated;mutations in the human homolog are associatedwith lactic acidosis &radic0.514770.95304&radic&radic&radic&radic S000003666URA2YJL130CverifiedBifunctional carbamoylphosphate synthetase (CPSase)-aspartatetranscarbamylase (ATCase), catalyzes the firsttwo enzymatic steps in the de novo biosynthesisof pyrimidines; both activities are subject tofeedback inhibition by UTP &radic0.502240.95058&radic&radic&radic&radic S000000245FAT1YBR041WverifiedFatty acid transporter and very long-chain fatty acyl-CoAsynthetase, may form a complex with Faa1p orFaa4p that imports and activates exogenousfatty acids &radic0.502190.95058&radic S000004860FAA4YMR246WverifiedLong chain fatty acyl-CoA synthetase, regulates proteinmodification during growth in the presence ofethanol, functions to incorporate palmitic acidinto phospholipids and neutral lipids &radic0.493940.94787&radic&radic&radic S000005844FAA1YOR317WverifiedLong chain fatty acyl-CoA synthetase with a preference forC12:0-C16:0 fatty acids; involved in theactivation of imported fatty acids; localizedto both lipid particles and mitochondrial outermembrane; essential for stationary phase &radic0.491220.94787&radic S000000422PYC2YBR218CverifiedPyruvate carboxylase isoform, cytoplasmic enzyme that convertspyruvate to oxaloacetate; highly similar toisoform Pyc1p but differentially regulated;mutations in the human homolog are associatedwith lactic acidosis &radic0.491420.94787&radic&radic&radic&radic&radic S000003496MES1YGR264CverifiedMethionyl-tRNA synthetase, forms a complex with glutamyl-tRNAsynthetase (Gus1p) and Arc1p, which increasesthe catalytic efficiency of both tRNAsynthetases; also has a role in nuclear exportof tRNAs &radic0.484180.9465&radic&radic&radic&radic S000003417TYS1YGR185CverifiedCytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNAsynthetase that aminoacylates tRNA(Tyr),required for cytoplasmic protein synthesis,interacts with positions 34 and 35 of theanticodon of tRNATyr &radic0.477490.9465&radic&radic&radic&radic S000003214GUS1YGL245WverifiedGlutamyl-tRNA synthetase (GluRS), forms a complex withmethionyl-tRNA synthetase (Mes1p) and Arc1p;complex formation increases the catalyticefficiency of both tRNA synthetases and ensurestheir correct localization to the cytoplasm &radic0.483230.9465&radic&radic&radic&radic&radic&radic&radic S000000618SLM5YCR024CverifiedMitochondrial asparaginyl-tRNA synthetase &radic0.474440.94327&radic S000006081CDC60YPL160WverifiedCytosolic leucyl tRNA synthetase, ligates leucine to theappropriate tRNA &radic0.471530.94224&radic&radic&radic&radic&radic&radic&radic&radic S000001493UFD4YKL010CverifiedUbiquitin-protein ligase (E3) that interacts with Rpt4p andRpt6p, two subunits of the 19S particle of the26S proteasome; cytoplasmic E3 involved in thedegradation of ubiquitin fusion proteins &radic0.468450.94032&radic&radic&radic&radic&radic S000003941DPS1YLL018CverifiedCytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme thatcatalyzes the specific aspartylation oftRNA(Asp); class II aminoacyl tRNA synthetase;binding to its own mRNA may conferautoregulation &radic0.459270.93754&radic&radic&radic&radic&radic S000003209DOC1YGL240WverifiedProcessivity factor required for the ubiquitination activity ofthe anaphase promoting complex (APC), mediatesthe activity of the APC by contributing tosubstrate recognition; involved in cyclinproteolysis 0.448080.93584&radic&radic&radic&radic&radic&radic&radic S000003583RTT101YJL047CverifiedCullin subunit of a Roc1p-dependent E3 ubiquitin ligasecomplex; deletion phenotype suggests a role inanaphase progression; interacts with Mms22p andimplicated in Mms22-dependent DNA repair;modified by the ubiquitin-like protein, Rub1p &radic0.444620.93584&radic&radic S000003326VAS1YGR094WverifiedMitochondrial and cytoplasmic valyl-tRNA synthetase &radic0.443680.93584&radic&radic&radic&radic&radic&radic S000005961ISM1YPL040CverifiedMitochondrial isoleucyl-tRNA synthetase, null mutant isdeficient in respiratory growth &radic0.441230.9357&radic&radic&radic&radic S000004820HFA1YMR207CverifiedMitochondrial acetyl-coenzyme A carboxylase, catalyzes theproduction of malonyl-CoA in mitochondrialfatty acid biosynthesis &radic0.441930.9357 S000006285GRS2YPR081CverifiedProtein with sequence similarity to Grs1p, which is aglycyl-tRNA synthetase; cannot substitute forGrs1p; possible pseudogene that is expressed atvery low levels &radic0.432890.93556&radic S000001677MST1YKL194CverifiedMitochondrial threonyl-tRNA synthetase &radic0.427340.93241 S000000325GRS1YBR121CverifiedCytoplasmic and mitochondrial glycyl-tRNA synthase that ligatesglycine to the cognate anticodon bearing tRNA;transcription termination factor that mayinteract with the 3'-end of pre-mRNA to promote3'-end formation &radic0.429060.93241&radic&radic S000005299ACC1YNR016CverifiedAcetyl-CoA carboxylase, biotin containing enzyme that catalyzesthe carboxylation of acetyl-CoA to formmalonyl-CoA; required for de novo biosynthesisof long-chain fatty acids &radic0.424640.93183&radic&radic&radic&radic&radic&radic&radic S000003403MSM1YGR171CverifiedMitochondrial methionyl-tRNA synthetase (MetRS), functions as amonomer in mitochondrial protein synthesis;functions similarly to cytoplasmic MetRSalthough the cytoplasmic form contains azinc-binding domain not found in Msm1p &radic0.418290.92767&radic S000005457WRS1YOL097CverifiedCytoplasmic tryptophanyl-tRNA synthetase, aminoacylatestryptophanyl-tRNA &radic0.417530.92767&radic&radic&radic S000005191YNL247WUncharacterizedProtein required for cell viability &radic0.413630.92767&radic&radic&radic&radic&radic S000001133MSR1YHR091CverifiedMitochondrial arginyl-tRNA synthetase &radic0.41140.92719&radic&radic&radic S000006237HTS1YPR033CverifiedCytoplasmic and mitochondrial histidine tRNA synthetase;encoded by a single nuclear gene that specifiestwo messages; efficient mitochondriallocalization requires both a presequence and anamino-terminal sequence &radic0.405530.92337&radic S000002749YDR341CVerifiedCytoplasmic arginyl-tRNA synthetase &radic0.403750.92273&radic&radic&radic&radic S000000889YER087WUncharacterizedHypothetical protein &radic0.394750.91864&radic&radic S000001340THS1YIL078WverifiedThreonyl-tRNA synthetase, essential cytoplasmic protein &radic0.386120.91557&radic&radic&radic S000002676MSW1YDR268WverifiedMitochondrial tryptophanyl-tRNA synthetase &radic0.386930.91557&radic&radic S000002817SIZ1YDR409WverifiedSUMO ligase that promotes the attachment of sumo (Smt3p; smallubiquitin-related modifier) to proteins; bindsUbc9p and may bind septins; specificallyrequired for sumoylation of septins in vivo;localized to the septin ring &radic0.377550.91246&radic&radic&radic S000001209CDC23YHR166CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.374470.91136&radic S000006025MSD1YPL104WverifiedMitochondrial aspartyl-tRNA synthetase, required for acylationof aspartyl-tRNA; yeast and bacterialaspartyl-, asparaginyl-, and lysyl-tRNAsynthetases contain regions with high sequencesimilarity, suggesting a common ancestral gene &radic0.366380.90476&radic&radic S000005682NFI1YOR156CverifiedSUMO ligase, catalyzes the covalent attachment of SUMO (Smt3p)to proteins; involved in maintenance of propertelomere length &radic0.367810.90476&radic&radic&radic&radic S000006018MSY1YPL097WverifiedMitochondrial tyrosyl-tRNA synthetase &radic0.369530.90476&radic&radic&radic&radic S000002444KRS1YDR037WverifiedLysyl-tRNA synthetase; also identified as a negative regulatorof general control of amino acid biosynthesis &radic0.362240.90362&radic&radic S000005694GLN4YOR168WverifiedGlutamine tRNA synthetase, monomeric class I tRNA synthetasethat catalyzes the specific glutaminylation oftRNA(Glu); N-terminal domain proposed to beinvolved in enzyme-tRNA interactions &radic0.355130.90242&radic&radic&radic&radic&radic&radic S000003870CPA2YJR109CverifiedLarge subunit of carbamoyl phosphate synthetase, whichcatalyzes a step in the synthesis ofcitrulline, an arginine precursor &radic0.35370.90157&radic S000000412DUR1,2YBR208CverifiedUrea amidolyase, contains both urea carboxylase and allophanatehydrolase activities, degrades urea to CO2 andNH3; expression sensitive to nitrogencatabolite repression and induced byallophanate, an intermediate in allantoindegradation &radic0.347650.89744&radic&radic&radic&radic&radic S000005017MSK1YNL073WverifiedMitochondrial lysine-tRNA synthetase, required for import ofboth aminoacylated and deacylated forms oftRNA(Lys) into mitochondria &radic0.345880.89669 S000004143ACS2YLR153CverifiedAcetyl-coA synthetase isoform which, along with Acs1p, is thenuclear source of acetyl-coA for histoneacetlyation; mutants affect globaltranscription; required for growth on glucose;expressed under anaerobic conditions &radic0.33660.89265 S000003797HUL4YJR036CverifiedProtein with similarity to hect domain E3 ubiquitin-proteinligases, not essential for viability &radic0.33630.89215&radic S000002865TOM1YDR457WverifiedE3 ubiquitin ligase of the hect-domain class; has a role inmRNA export from the nucleus and may regulatetranscriptional coactivators &radic0.330980.88888&radic S000005393MSE1YOL033WverifiedMitochondrial glutamyl-tRNA synthetase, encoded by a nucleargene &radic0.328630.88888&radic&radic&radic&radic&radic&radic S000001053DIA4YHR011WverifiedProbable mitochondrial seryl-tRNA synthetase, mutant displaysincreased invasive and pseudohyphal growth &radic0.331280.88888&radic&radic&radic&radic S000003109HUL5YGL141WverifiedProtein with similarity to hect domain E3 ubiquitin-proteinligases, not essential for viability &radic0.324810.88721&radic S000000135URA7YBL039CverifiedMajor CTP synthase isozyme (see also URA8), catalyzes theATP-dependent transfer of the amide nitrogenfrom glutamine to UTP, forming CTP, the finalstep in de novo biosynthesis of pyrimidines;involved in phospholipid biosynthesis &radic0.319710.8851&radic S000002430SES1YDR023WverifiedCytosolic seryl-tRNA synthetase, class II aminoacyl-tRNAsynthetase that aminoacylates tRNA(Ser),displays tRNA-dependent amino acid recognitionwhich enhances discrimination of the serinesubstrate, interacts with peroxin Pex21p &radic0.315870.88408&radic&radic S000001505CDC16YKL022CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; required forsporulation &radic0.315150.88386&radic&radic&radic&radic&radic&radic&radic S000004374NAM2YLR382CverifiedMitochondrial leucyl-tRNA synthetase, also has a direct role insplicing of several mitochondrial group Iintrons; indirectly required for mitochondrialgenome maintenance &radic0.311960.88211 S000000319LYS2YBR115CverifiedAlpha aminoadipate reductase, catalyzes the reduction ofalpha-aminoadipate to alpha-aminoadipate6-semialdehyde, which is the fifth step inbiosynthesis of lysine; activation requiresposttranslational phosphopantetheinylation byLys5p 0.313270.88211&radic&radic S000003235CUL3YGR003WverifiedUbiquitin-protein ligase, member of the cullin family withsimilarity to Cdc53p and human CUL3; nullmutation has no apparent phenotype &radic0.311350.88149&radic&radic&radic S000000426PCS60YBR222CverifiedPeroxisomal AMP-binding protein, localizes to both theperoxisomal peripheral membrane and matrix,expression is highly inducible by oleic acid,similar to E. coli long chain acyl-CoAsynthetase 0.308530.88047&radic&radic&radic&radic&radic S000002691GCN2YDR283CverifiedProtein kinase, phosphorylates the alpha-subunit of translationinitiation factor eIF2 (Sui2p) in response tostarvation; activated by uncharged tRNAs andthe Gcn1p-Gcn20p complex 0.280990.86688&radic&radic&radic S000000180CDC27YBL084CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.28030.86688&radic&radic&radic S000005654ADE2YOR128CverifiedPhosphoribosylaminoimidazole carboxylase, catalyzes a step inthe 'de novo' purine nucleotide biosyntheticpathway; red pigment accumulates in mutantcells deprived of adenine 0.27850.86545&radic&radic&radic&radic&radic S000000452HIS7YBR248CverifiedImidazole glycerol phosphate synthase (glutamineamidotransferase:cyclase), catalyzes the fifthand sixth steps of histidine biosynthesis andalso produces 5-aminoimidazole-4-carboxamideribotide (AICAR), a purine precursor 0.273670.86213&radic&radic&radic&radic&radic S000001855ACT1YFL039CverifiedActin, structural protein involved in cell polarization,endocytosis, and other cytoskeletal functions 0.272490.8613&radic&radic&radic S000001872FRS2YFL022CverifiedAlpha subunit of cytoplasmic phenylalanyl-tRNA synthetase,forms a tetramer with Frs1p to form activeenzyme; evolutionarily distant frommitochondrial phenylalanyl-tRNA synthetasebased on protein sequence, but substratebinding is similar &radic0.266770.85891&radic&radic&radic S000005116APC1YNL172WverifiedLargest subunit of the Anaphase-Promoting Complex/Cyclosome(APC/C), which is a ubiquitin-protein ligaserequired for degradation of anaphaseinhibitors, including mitotic cyclins, duringthe metaphase/anaphase transition &radic0.25470.85177&radic&radic&radic&radic&radic&radic S000003356ASN2YGR124WverifiedAsparagine synthetase, isozyme of Asn1p; catalyzes thesynthesis of L-asparagine from L-aspartate inthe asparagine biosynthetic pathway &radic0.250410.84839&radic&radic&radic&radic S000001271FAA3YIL009WverifiedLong chain fatty acyl-CoA synthetase, has a preference for C16and C18 fatty acids; green fluorescent protein(GFP)-fusion protein localizes to the cellperiphery &radic0.2380.8382&radic S000000817FAA2YER015WverifiedLong chain fatty acyl-CoA synthetase; accepts a wider range ofacyl chain lengths than Faa1p, preferringC9:0-C13:0; involved in the activation ofendogenous pools of fatty acids &radic0.237450.83782 S000006349ASN1YPR145WverifiedAsparagine synthetase, isozyme of Asn2p; catalyzes thesynthesis of L-asparagine from L-aspartate inthe asparagine biosynthetic pathway &radic0.237350.83738&radic&radic&radic S000003864URA8YJR103WverifiedMinor CTP synthase isozyme (see also URA7), catalyzes theATP-dependent transfer of the amide nitrogenfrom glutamine to UTP, forming CTP, the finalstep in de novo biosynthesis of pyrimidines;involved in phospholipid biosynthesis &radic0.225160.82974&radic S000001694TRP3YKL211CverifiedBifunctional enzyme exhibiting both indole-3-glycerol-phosphatesynthase and anthranilate synthase activities,forms multifunctional hetero-oligomericanthranilate synthase:indole-3-glycerolphosphate synthase enzyme complex with Trp2p 0.225570.82974&radic&radic&radic&radic&radic&radic&radic&radic S000006251MSF1YPR047WverifiedMitochondrial phenylalanyl-tRNA synthetase alpha subunit,active as a monomer, unlike the cytoplasmicsubunit which is active as a dimer complexed toa beta subunit dimer; similar to the alphasubunit of E. coli phenylalanyl-tRNA synthetase &radic0.223840.82956 S000005829CPA1YOR303WverifiedSmall subunit of carbamoyl phosphate synthetase, whichcatalyzes a step in the synthesis ofcitrulline, an arginine precursor;translationally regulated by an attenuatorpeptide encoded by YOR302W within the CPA1 mRNA5'-leader &radic0.213420.82171&radic S000004719FOL3YMR113WverifiedDihydrofolate synthetase, involved in folic acid biosynthesis;catalyzes the conversion of dihydropteroate todihydrofolate in folate coenzyme biosynthesis &radic0.203330.81379&radic&radic S000003436ADE3YGR204WverifiedCytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase,involved in single carbon metabolism andrequired for biosynthesis of purines,thymidylate, methionine, and histidine &radic0.199210.81093&radic&radic&radic S000000288MIS1YBR084WverifiedMitochondrial C1-tetrahydrofolate synthase, involved ininterconversion between different oxidationstates of tetrahydrofolate (THF); providesactivities of formyl-THF synthetase,methenyl-THF cyclohydrolase, and methylene-THFdehydrogenase &radic0.197590.81003 S000005767MET7YOR241WverifiedFolylpolyglutamate synthetase, catalyzes extension of theglutamate chains of the folate coenzymes,required for methionine synthesis and formaintenance of mitochondrial DNA &radic0.194020.80868&radic&radic&radic&radic&radic&radic&radic&radic S000000050ACS1YAL054CverifiedAcetyl-coA synthetase isoform which, along with Acs2p, is thenuclear source of acetyl-coA for histoneacetlyation; expressed during growth onnonfermentable carbon sources and under aerobicconditions &radic0.192070.80781&radic&radic S000004116YLR126CUncharacterizedPutative protein of unknown function with similarity to glutamine amidotransferase proteins; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein 0.19120.80745&radic&radic&radic&radic S000003203ADE5,7YGL234WverifiedBifunctional enzyme of the 'de novo' purine nucleotidebiosynthetic pathway, contains aminoimidazoleribotide synthetase and glycinamide ribotidesynthetase activities &radic0.186510.80103&radic&radic S000005255SKP2YNL311CverifiedPutative F-box protein; analysis of integrated high-throughputdatasets predicts an involvement inubiquitin-dependent protein catabolism 0.176290.78875&radic&radic&radic&radic S000003582YJL046WUncharacterizedPutative protein of unknown function, predicted to encode a lipoate-protein ligase A family member; deletion mutant has a respiratory growth defect 0.171020.78307&radic S000003476LSC2YGR244CverifiedBeta subunit of succinyl-CoA ligase, which is a mitochondrialenzyme of the TCA cycle that catalyzes thenucleotide-dependent conversion of succinyl-CoAto succinate &radic0.16880.78161 S000005606DIA2YOR080WverifiedOrigin-binding F-box protein that forms an SCF ubiquitin ligasecomplex with Skp1p and Cdc53p; plays a role inDNA replication, involved in invasive andpseudohyphal growth 0.165150.77707&radic&radic&radic&radic&radic S000004830GUA1YMR217WverifiedGMP synthase, an enzyme that catalyzes the second step in thebiosynthesis of GMP from inosine 5'-phosphate(IMP); transcription is not subject toregulation by guanine but is negativelyregulated by nutrient starvation &radic0.163280.77396&radic&radic S000001615RMA1YKL132CverifiedPutative dihydrofolate synthetase; has similarity to Fol3p andto E. coli folylpolyglutamatesynthetase/dihydrofolate synthetase; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies &radic0.162680.77369&radic&radic&radic&radic&radic S000005373HRD1YOL013CverifiedUbiquitin-protein ligase required for endoplasmicreticulum-associated degradation (ERAD) ofmisfolded proteins; genetically linked to theunfolded protein response (UPR); regulatedthrough association with Hrd3p; contains an H2ring finger &radic0.158170.76694&radic&radic S000002340INH1YDL181WverifiedProtein that inhibits ATP hydrolysis by the F1F0-ATP synthase,inhibitory function is enhanced by stabilizingproteins Stf1p and Stf2p; has similarity toStf1p and both Inh1p and Stf1p exhibit thepotential to form coiled-coil structures 0.157170.76597&radic S000001308MET30YIL046WverifiedF-box protein containing five copies of the WD40 motif,controls cell cycle function, sulfurmetabolism, and methionine biosynthesis as partof the ubiquitin ligase complex; interacts withand regulates Met4p, localizes within thenucleus 0.151260.75813&radic&radic S000002323CDC9YDL164CverifiedDNA ligase found in the nucleus and mitochondria, an essentialenzyme that joins Okazaki fragments during DNAreplication; also acts in nucleotide excisionrepair, base excision repair, and recombination &radic0.14520.74786&radic&radic S000000745MMS21YEL019CverifiedSUMO ligase involved in chromosomal organization and DNArepair; essential subunit of theMms21-Smc5-Smc6 complex; mutants are sensitiveto methyl methanesulfonate and show increasedspontaneous mutation and mitotic recombination &radic0.142920.74623&radic&radic&radic&radic&radic&radic S000005493HRT1YOL133WverifiedRING finger containing subunit of Skp1-Cullin-F-box ubiquitinprotein ligases (SCF); required for Gic2p,Far1p, Sic1p and Cln2p degradation; may tetherCdc34p (a ubiquitin conjugating enzyme or E2)and Cdc53p (a cullin) subunits of SCF &radic0.142660.74623&radic&radic S000000949SCC4YER147CverifiedSubunit of cohesin loading factor (Scc2p-Scc4p), a complexrequired for the loading of cohesin complexesonto chromosomes; involved in establishingsister chromatid cohesion during double-strandbreak repair via phosphorylated histone H2AX 0.139790.73995&radic S000005316ABZ1YNR033WverifiedPara-aminobenzoate (PABA) synthase, has similarity toEscherichia coli PABA synthase components PabAand PabB &radic0.138520.73781&radic&radic S000003416UBR1YGR184CverifiedUbiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2pto ubiquitinate substrates of the N-end rulepathway; binds to the Rpn2p, Rpt1p, and Rpt6pproteins of the 19S particle of the 26Sproteasome &radic0.138510.73753&radic&radic S000001788MTD1YKR080WverifiedNAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase,plays a catalytic role in oxidation ofcytoplasmic one-carbon units; expression isregulated by Bas1p and Bas2p, repressed byadenine, and may be induced by inositol andcholine 0.135390.73081&radic&radic S000003685YJL149WVerifiedPutative SCF ubiquitin ligase F-box protein of unknown function; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C; YJL149W is a non-essential gene 0.135810.73081&radic&radic&radic S000005619YOR093CUncharacterizedHypothetical protein 0.134980.73027&radic&radic&radic&radic S000004087HRT3YLR097CuncharacterizedPutative nuclear ubiquitin ligase, based on computationalanalysis of large-scale protein-proteininteraction data; has similarity to F-boxproteins; identified in association withCdc53p, Skp1p and Ubi4 in large-scale studies 0.131880.72649&radic&radic&radic S000000065RFA1YAR007CverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 0.127790.71978&radic&radic&radic S000004014UBR2YLR024CverifiedCytoplasmic ubiquitin-protein ligase (E3) &radic0.126660.71932&radic&radic S000004419MAG2YLR427WuncharacterizedDNA-3-methyladenine glycosidase II that catalyzes of thehydrolysis of alkylated DNA 0.126010.71875&radic&radic S000004237IRC20YLR247CuncharacterizedPutative helicase; localized to mitochondria and the nucleus;YLR247C is not an essential gene; null mutantdisplays increased levels of spontaneous Rad52foci 0.118570.70621&radic&radic&radic S000001517TUL1YKL034WverifiedGolgi-localized RING-finger ubiquitin ligase (E3), involved inubiquitinating and sorting membrane proteinsthat contain polar transmembrane domains tomultivesicular bodies for delivery to thevacuole for quality control purposes &radic0.116840.70504&radic&radic&radic&radic&radic S000000662RAD18YCR066WverifiedProtein involved in postreplication repair; bindssingle-stranded DNA and has single-stranded DNAdependent ATPase activity; forms heterodimerwith Rad6p; contains RING-finger motif 0.114680.70014 S000004562YML096WUncharacterizedPutative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 0.113980.69917&radic S000004138PEP3YLR148WverifiedVacuolar peripheral membrane protein that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, required for vacuolarbiogenesis, forms complex with Pep5p thatmediates protein transport to the vacuole 0.112160.69721&radic&radic&radic S000004022RAD5YLR032WverifiedSingle-stranded DNA-dependent ATPase, involved inpostreplication repair; contains RING fingerdomain 0.112460.69721&radic S000004402VIP1YLR410WverifiedProtein of unknown function probably involved in the functionof the cortical actin cytoskeleton; putativeortholog of S. pombe asp1+ 0.112220.69721&radic&radic&radic&radic&radic S000005409GSH2YOL049WverifiedGlutathione synthetase, catalyzes the ATP-dependent synthesisof glutathione (GSH) fromgamma-glutamylcysteine and glycine; induced byoxidative stress and heat shock &radic0.111160.69547&radic S000003740RCY1YJL204CverifiedF-box protein involved in recycling plasma membrane proteinsinternalized by endocytosis; localized to sitesof polarized growth 0.107180.68889&radic&radic&radic&radic&radic S000005531DNL4YOR005CverifiedDNA ligase required for nonhomologous end-joining (NHEJ), formsstable heterodimer with required cofactorLif1p, catalyzes DNA ligation as part of acomplex with Lif1p and Nej1p; involved inmeiosis, not essential for vegetative growth &radic0.106990.68882&radic S000002166APC11YDL008WverifiedCatalytic core subunit of the Anaphase-PromotingComplex/Cyclosome (APC/C), which is aubiquitin-protein ligase required fordegradation of anaphase inhibitors, includingmitotic cyclins, during the metaphase/anaphasetransition &radic0.106940.68882 S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.106110.68765&radic&radic&radic S000000318RAD16YBR114WverifiedProtein that recognizes and binds damaged DNA in anATP-dependent manner (with Rad7p) duringnucleotide excision repair; subunit ofNucleotide Excision Repair Factor 4 (NEF4);member of the SWI/SNF family 0.102490.68318&radic S000002673PEX10YDR265WverifiedRING finger peroxisomal membrane peroxin required forperoxisomal matrix protein import, interactswith Pex12p, links ubiquitin-conjugating Pex4pto protein import machinery; mutations in humanhomolog cause a variety of peroxisomaldisorders 0.100710.68009 S000001885CDC4YFL009WverifiedF-box protein required for G1/S and G2/M transition, associateswith Skp1p and Cdc53p to form a complex,SCFCdc4, which acts as ubiquitin-protein ligasedirecting ubiquitination of the phosphorylatedCDK inhibitor Sic1p &radic0.09950.67713&radic&radic&radic S000004360MDM30YLR368WverifiedF-box protein; physically associates with mitochondria and isrequired for normal mitochondrial fusion inrich medium, during sporulation, and in matingcells; promotes ubiquitin-mediated degradationof Gal4p in some strains 0.099390.67713&radic&radic S000004861RKR1YMR247CverifiedProtein of unknown function; may interact with ribosomes, basedon co-purification experiments; YMR247C is notan essential gene 0.095720.67265&radic&radic&radic&radic S000000484SAF1YBR280CverifiedF-Box protein involved in proteasome-dependent degradation ofAah1p during entry of cells into quiescence;interacts with Skp1 0.094520.67103 S000004315CWC24YLR323CverifiedEssential protein, component of a complex containing Cef1p; hassimilarity to S. pombe Cwf24p 0.091970.66561&radic&radic S000002362ACK1YDL203CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.090330.66062 S000002627MFB1YDR219CverifiedMitochondria-associated F-box protein involved in maintenanceof normal mitochondrial morphology; interactswith Skp1p through the F-box motif;preferentially localizes to the mother cellduring budding 0.091150.66062&radic&radic&radic&radic S000001002YHL010CUncharacterizedPutative protein of unknown function, contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences 0.087920.65688&radic&radic&radic&radic&radic S000000918SLX8YER116CverifiedProtein containing a RING finger domain that interacts withHex3p; mutant phenotypes and geneticinteractions suggest a role in sumoylation andin genome stability 0.086630.65461&radic S000001157DMA1YHR115CverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma2p;homolog of S. pombe Dma1 and H. sapiens Chfr 0.085330.65093&radic&radic&radic&radic S000005256RFA2YNL312WverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 0.083840.64859&radic&radic&radic S000006297ASR1YPR093CverifiedProtein involved in a putative alcohol-responsive signalingpathway; accumulates in the nucleus underalcohol stress; contains a Ring/PHD fingerdomain 0.083240.64859&radic S000004968FAP1YNL023CverifiedProtein that binds to Fpr1p (FKBP12), conferring rapamycinresistance by competing with rapamycin forFpr1p binding; has similarity to putativetranscription factors, including D.melanogaster shuttle craft and human NFX1 0.081480.64496 S000000002VPS8YAL002WverifiedMembrane-associated hydrophilic protein that interacts with thesmall GTPase, Vps21p, to facilitate solublevacuolar protein localization; required forlocalization and trafficking of the CPY sortingreceptor; contains a RING finger motif 0.081560.64496&radic&radic&radic S000003850GRR1YJR090CverifiedF-box protein component of the SCF ubiquitin-ligase complex,required for Cln1p and Cln2p degradation;involved in carbon catabolite repression,glucose-dependent divalent cation transport,high-affinity glucose transport, andmorphogenesis &radic0.08120.64454 S000005498YOL138CUncharacterizedHypothetical protein 0.080370.64262 S000000298PBY1YBR094WuncharacterizedP-body associated protein 0.077170.63507&radic&radic&radic S000005967ELC1YPL046CverifiedElongin C, forms heterodimer with Ela1p that participates intranscription elongation; expressiondramatically upregulated during sporulation;widely conserved among eukaryotes 0.076740.63488&radic&radic&radic&radic&radic S000000407COS111YBR203WverifiedProtein required for resistance to the antifungal drugciclopirox olamine; not related to thesubtelomerically-encoded COS family; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 0.074210.62784&radic&radic&radic&radic&radic S000005415PSH1YOL054WuncharacterizedNuclear protein, putative RNA polymerase II elongation factor;isolated as Pob3p/Spt16p-binding protein 0.074120.62784&radic&radic&radic&radic&radic S000002721PIB1YDR313CverifiedRING-type ubiquitin ligase of the endosomal and vacuolarmembranes, bindsphosphatidylinositol(3)-phosphate; contains aFYVE finger domain &radic0.073990.62768&radic&radic S000004844PEP5YMR231WverifiedPeripheral vacuolar membrane protein required for proteintrafficking and vacuole biogenesis; formscomplex with Pep3p that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, also interacts with Pep7p 0.072970.62441&radic&radic&radic&radic S000000266YBR062CUncharacterizedHypothetical protein 0.071510.62214 S000003693FAR1YJL157CverifiedCyclin-dependent kinase inhibitor that mediates cell cyclearrest in response to pheromone; also forms acomplex with Cdc24p, Ste4p, and Ste18p that mayspecify the direction of polarized growthduring mating; potential Cdc28p substrate 0.069890.6169&radic&radic&radic&radic S000005668LSC1YOR142WverifiedAlpha subunit of succinyl-CoA ligase, which is a mitochondrialenzyme of the TCA cycle that catalyzes thenucleotide-dependent conversion of succinyl-CoAto succinate &radic0.069060.61546&radic&radic&radic&radic S000004206CPR6YLR216CverifiedPeptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzesthe cis-trans isomerization of peptide bondsN-terminal to proline residues; binds to Hsp82pand contributes to chaperone activity 0.068590.61462&radic&radic&radic&radic&radic&radic S000004985YNL040WUncharacterizedPutative protein of unknown function with strong similarity to alanyl-tRNA synthases from Eubacteria; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene 0.068440.61219&radic&radic S000004553UFO1YML088WverifiedF-box receptor protein, subunit of the Skp1-Cdc53-F-boxreceptor (SCF) E3 ubiquitin ligase complex;binds to phosphorylated Ho endonuclease,allowing its ubiquitylation by SCF andsubsequent degradation &radic0.068510.61219&radic S000004229LIP2YLR239CverifiedLipoyl ligase, involved in the modification of mitochondrialenzymes by the attachment of lipoic acid groups &radic0.067190.61039&radic&radic&radic&radic&radic S000003746PEX2YJL210WverifiedRING-finger peroxin, peroxisomal membrane protein with aC-terminal zinc-binding RING domain, formsputative translocation subcomplex with Pex10pand Pex12p which functions in peroxisomalmatrix protein import 0.065520.60277&radic&radic S000000870MOT2YER068WverifiedComponent of the CCR4-NOT complex, which has multiple roles intranscription regulation, mRNA degradation, andpost-transcriptional modifications; with Ubc4p,ubiquitylates subunits of the nascentpolypeptide-associated complex (NAC) 0.063790.59872&radic&radic&radic S000001587GFA1YKL104CverifiedGlutamine-fructose-6-phosphate amidotransferase, catalyzes theformation of glucosamine-6-P and glutamate fromfructose-6-P and glutamine in the first step ofchitin biosynthesis 0.06270.59532&radic&radic&radic S000000157SKT5YBL061CverifiedActivator of Chs3p (chitin synthase III), recruits Chs3p to thebud neck via interaction with Bni4p; hassimilarity to Shc1p, which activates Chs3pduring sporulation 0.061490.59166&radic&radic&radic&radic&radic&radic S000004344YLR352WUncharacterizedPutative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene 0.060810.58971&radic S000002674YDR266CUncharacterizedHypothetical protein 0.060620.58908&radic&radic&radic S000002714YDR306CUncharacterizedF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain 0.060020.58668&radic&radic&radic S000000898SHC1YER096WverifiedSporulation-specific activator of Chs3p (chitin synthase III),required for the synthesis of the chitosanlayer of ascospores; has similarity to Skt5p,which activates Chs3p during vegetative growth;transcriptionally induced at alkaline pH 0.060440.58668 S000003293ADE6YGR061CverifiedFormylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzesa step in the 'de novo' purine nucleotidebiosynthetic pathway &radic0.059590.58567&radic&radic&radic&radic&radic S000002232BRE1YDL074CverifiedE3 ubiquitin ligase for Rad6p, required for the ubiquitinationof histone H2B, recruitment of Rad6p topromoter chromatin and subsequent methylationof histone H3 (on L4 and L79), contains RINGfinger domain &radic0.058180.58114 S000004533ITT1YML068WverifiedProtein that modulates the efficiency of translationtermination, interacts with translation releasefactors eRF1 (Sup45p) and eRF3 (Sup35p) invitro, contains a zinc finger domaincharacteristic of the TRIAD class of proteins 0.055720.56997 S000002868TFB3YDR460WverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, involved in transcriptioninitiation, required for nucleotide excisionrepair; ring finger protein similar tomammalian CAK and TFIIH subunit 0.054430.56388 S000002550SAN1YDR143CverifiedUbiquitin-protein ligase, involved in the proteasome-dependentdegradation of aberrant nuclear proteins; san1mutations suppress sir4, spt16, and cdc68mutations, suggesting a role in chromatinsilencing &radic0.053230.56129&radic&radic&radic S000004605YMR003WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria; YMR003W is not an essential gene 0.05150.55451&radic&radic&radic S000000211DSF2YBR007CuncharacterizedDeletion suppressor of mpt5 mutation 0.051160.55369 S000000892TRP2YER090WverifiedAnthranilate synthase, catalyzes the initial step of tryptophanbiosynthesis, forms multifunctionalhetero-oligomeric anthranilatesynthase:indole-3-glycerol phosphate synthaseenzyme complex with Trp3p 0.049090.54414&radic S000000070ADE1YAR015WverifiedN-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR)synthetase, required for 'de novo' purinenucleotide biosynthesis; red pigmentaccumulates in mutant cells deprived of adenine &radic0.047440.53756&radic&radic&radic&radic&radic&radic&radic&radic S000005583SGT1YOR057WverifiedProbable cochaperone, regulates activity of Cyr1p (adenylylcyclase); involved in assembly of thekinetochore complex, associates with the SCF(Skp1p/Cdc53p/F box protein) ubiquitin ligasecomplex 0.046690.53286&radic&radic&radic S000005060DMA2YNL116WverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma1p;homolog of S. pombe Dma1 and H. sapiens Chfr 0.045290.52774&radic&radic S000000865THO1YER063WverifiedProtein of unknown function; overproduction suppresses thetranscriptional defect caused by an hpr1mutation 0.042510.51546 S000005257YNL313CUncharacterizedProtein required for cell viability 0.038120.4892&radic&radic&radic S000001243RPN10YHR200WverifiedNon-ATPase base subunit of the 19S regulatory particle (RP) ofthe 26S proteasome; N-terminus plays a role inmaintaining the structural integrity of the RP;binds selectively to polyubiquitin chains;homolog of the mammalian S5a protein 0.037670.48822&radic&radic&radic&radic&radic S000005135DUG3YNL191WuncharacterizedProtein involved in alternative pathway of glutathionedegradation 0.035910.47568&radic&radic&radic&radic&radic S000002171HEX3YDL013WverifiedProtein containing a RING finger domain that interacts withSlx8p; mutant phenotypes and geneticinteractions suggest a role in sumoylation andin genome stability 0.035760.47253&radic S000002555KGD2YDR148CverifiedDihydrolipoyl transsuccinylase, a component of themitochondrial alpha-ketoglutarate dehydrogenasecomplex, which catalyzes a step in thetricarboxylic acid (TCA) cycle, the oxidativedecarboxylation of alpha-ketoglutarate tosuccinyl-CoA 0.035680.47253&radic&radic S000002300BPL1YDL141WverifiedBiotin:apoprotein ligase, covalently modifies proteins with theaddition of biotin, required for acetyl-CoAcarboxylase (Acc1p) holoenzyme formation &radic0.034780.46956&radic&radic S000002731PEP7YDR323CverifiedMultivalent adaptor protein that facilitates vesicle-mediatedvacuolar protein sorting by ensuringhigh-fidelity vesicle docking and fusion, whichare essential for targeting of vesicles to theendosome; required for vacuole inheritance 0.033420.45872&radic&radic S000005553STI1YOR027WverifiedHsp90 cochaperone, interacts with the Ssa group of thecytosolic Hsp70 chaperones; activates theATPase activity of Ssa1p; homolog of mammalianHop protein 0.031860.44643&radic&radic&radic S000002538YDR131CUncharacterizedF-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex 0.030470.43126&radic&radic&radic&radic S000003099SNT2YGL131CverifiedDNA binding protein with similarity to the S. pombe Snt2protein 0.02990.42704&radic S000005289VPS27YNR006WverifiedEndosomal protein that forms a complex with Hse1p; required forrecycling Golgi proteins, forming lumenalmembranes and sorting ubiquitinated proteinsdestined for degradation; has UbiquitinInteraction Motifs which bind ubiquitin (Ubi4p) 0.028480.41274 S000005533SGT2YOR007CverifiedGlutamine-rich cytoplasmic protein of unknown function;contains tetratricopeptide (TPR) repeats, whichoften mediate protein-protein interactions; hassimilarity to human SGT, which is a cochaperonethat negatively regulates Hsp70 0.027490.40379&radic&radic&radic&radic&radic S000002652PEX5YDR244WverifiedPeroxisomal membrane signal receptor for C-terminal tripeptidesignal sequence (PTS1) of peroxisomal matrixproteins, required for peroxisomal matrixprotein import, tetratricopeptide repeatprotein, also involved in PTS1-independentimport 0.02740.40247&radic&radic&radic S000004814RAD14YMR201CverifiedProtein that recognizes and binds damaged DNA during nucleotideexcision repair; subunit of Nucleotide ExcisionRepair Factor 1 (NEF1); contains zinc fingermotif; homolog of human XPA protein 0.027190.4001&radic&radic&radic&radic&radic&radic S000005015LAT1YNL071WverifiedDihydrolipoamide acetyltransferase component (E2) of pyruvatedehydrogenase complex, which catalyzes theoxidative decarboxylation of pyruvate toacetyl-CoA 0.026910.39683 S000006128TYW1YPL207WuncharacterizedHypothetical protein 0.026410.39114&radic&radic&radic&radic&radic S000005174ELA1YNL230CverifiedElongin A, F-box protein that forms a heterodimer with Elc1pand participates in transcription elongation 0.026470.39114&radic&radic&radic&radic&radic&radic S000004915ADE4YMR300CverifiedPhosphoribosylpyrophosphate amidotransferase (PRPPAT;amidophosphoribosyltransferase), catalyzesfirst step of the 'de novo' purine nucleotidebiosynthetic pathway 0.025790.3851&radic&radic&radic S000001426NIT1YIL164CverifiedNitrilase, member of the widely found nitrilase branch(EC:3.5.5.1) of the nitrilase superfamily 0.02560.37932 S000002510STE5YDR103WverifiedPheromone-responsive scaffold protein; shuttles from thenucleus to the plasma membrane and assembleskinases Ste11p, Ste7p, and Fus3p into aspecific signaling complex; active oligomericform interacts with Ste4p-Ste18p complex 0.025180.37864&radic&radic&radic&radic S000000994HSE1YHL002WverifiedSubunit of the endosomal Vps27p-Hse1p complex required forsorting of ubiquitinated membrane proteins intointralumenal vesicles prior to vacuolardegradation, as well as for recycling of Golgiproteins and formation of lumenal membranes 0.024550.371 S000002728SWA2YDR320CverifiedAuxilin-like protein involved in vesicular transport;clathrin-binding protein required for uncoatingof clathrin-coated vesicles 0.024380.3687&radic&radic&radic&radic S000004628PEX12YMR026CverifiedRING-finger peroxisomal membrane peroxin that plays anessential role in peroxisome biogenesis andperoxisomal matrix protein import, formstranslocation subcomplex with Pex2p and Pex10p 0.024470.3687&radic&radic&radic&radic&radic S000003279TFC4YGR047CverifiedOne of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauA domain of TFIIIC that binds BoxA DNApromoter sites of tRNA and similar genes; hasTPR motifs; human homolog is TFIIIC-102 0.023970.36218&radic S000003823NTA1YJR062CverifiedAmidase, removes the amide group from N-terminal asparagine andglutamine residues to generate proteins withN-terminal aspartate and glutamate residuesthat are targets of ubiquitin-mediateddegradation 0.023710.35839&radic&radic S000002320ENT1YDL161WverifiedEpsin-like protein involved in endocytosis and actin patchassembly and functionally redundant with Ent2p;binds clathrin via a clathrin-binding domainmotif at C-terminus 0.023450.35667&radic S000005383IFM1YOL023WverifiedMitochondrial translation initiation factor 2 0.023310.35588&radic&radic S000004343NIT3YLR351CverifiedNit protein, one of two proteins in S. cerevisiae withsimilarity to the Nit domain of NitFhit fromfly and worm and to the mouse and human Nitprotein which interacts with the Fhit tumorsuppressor; nitrilase superfamily member 0.023230.35486&radic&radic S000003662NIT2YJL126WverifiedNit protein, one of two proteins in S. cerevisiae withsimilarity to the Nit domain of NitFhit fromfly and worm and to the mouse and human Nitprotein which interacts with the Fhit tumorsuppressor; nitrilase superfamily member 0.023060.35246&radic S000000120NCL1YBL024WverifiedS-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase,methylates cytosine to m5C at several positionsin tRNAs and intron-containing pre-tRNAs;similar to Nop2p and human proliferationassociated nucleolar protein p120 0.022680.34382&radic&radic&radic&radic&radic S000002991PIB2YGL023CverifiedProtein binding phosphatidylinositol 3-phosphate, involved intelomere-proximal repression of geneexpression; similar to Fab1 and Vps27 0.022540.34382&radic S000004196ENT2YLR206WverifiedEpsin-like protein required for endocytosis and actin patchassembly and functionally redundant with Ent1p;contains clathrin-binding motif at C-terminus 0.022640.34382&radic&radic&radic&radic S000004050FRS1YLR060WverifiedBeta subunit of cytoplasmic phenylalanyl-tRNA synthetase, formsa tetramer with Frs2p to generate activeenzyme; sequence is evolutionarily distant frommitochondrial phenylalanyl-tRNA synthetase(Msf1p), but substrate binding is similar &radic0.022390.34287&radic&radic&radic&radic&radic S000004575PML39YML107CverifiedProtein required for nuclear retention of unspliced pre-mRNAsalong with Mlp1p and Pml1p; anchored to nuclearpore complex via Mlp1p and Mlp2p; found withthe subset of nuclear pores farthest from thenucleolus; may interact with ribosomes 0.021980.33492 S000001292SSM4YIL030CverifiedUbiquitin-protein ligase of the ER/nuclear envelope, requiredfor degradation of Alpha2p and other proteinscontaining a Deg1 degradation signal; ssm4mutation suppresses mRNA instability caused byan rna14 mutation &radic0.022030.33492&radic S000003425PDX1YGR193CverifiedDihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) ofthe mitochondrial pyruvate dehydrogenase (PDH)complex, plays a structural role in the complexby binding and positioning E3 to thedihydrolipoamide acetyltransferase (E2) core 0.021350.32692&radic&radic&radic S000004620YMR018WUncharacterizedPutative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene 0.021140.32412&radic&radic&radic&radic S000003945HIF1YLL022CverifiedNon-essential component of the HAT-B histone acetyltransferasecomplex (Hat1p-Hat2p-Hif1p), localized to thenucleus; has a role in telomeric silencing 0.020510.31463 S000002824SYF1YDR416WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression; similar to Drosophila crooked neckprotein 0.020270.31054&radic&radic&radic&radic S000000340MEC1YBR136WverifiedGenome integrity checkpoint protein and PI kinase superfamilymember; signal transducer required for cellcycle arrest and transcriptional responsesprompted by damaged or unreplicated DNA;monitors and participates in meioticrecombination 0.020190.30972 S000006178YPL257WUncharacterizedHypothetical protein 0.020070.30422&radic&radic&radic&radic&radic S000005825BUD7YOR299WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p, from the Golgi to plasma membrane 0.019850.30422&radic&radic&radic&radic&radic S000000042GCV3YAL044CverifiedH subunit of the mitochondrial glycine decarboxylase complex,required for the catabolism of glycine to5,10-methylene-THF; expression is regulated bylevels of levels of 5,10-methylene-THF in thecytoplasm 0.019780.3034&radic&radic&radic&radic S000005775APC5YOR249CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.019150.29351&radic&radic&radic S000002777XRS2YDR369CverifiedProtein required for DNA repair; component of the Mre11complex, which is involved in double strandbreaks, meiotic recombination, telomeremaintenance, and checkpoint signaling 0.01870.28545&radic&radic&radic&radic&radic S000006393SKI3YPR189WverifiedProtein involved in exosome mediated 3' to 5' mRNA degradationand translation inhibition of non-poly(A)mRNAs; forms complex with Ski2p and Ski8p;required for repressing propagation of dsRNAviruses 0.018660.28545&radic&radic S000004850BCH1YMR237WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p; may interact with ribosomes 0.018520.28105&radic&radic&radic S000004760YIM1YMR152WverifiedProtein of unknown function; null mutant displays sensitivityto DNA damaging agents; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 0.01830.27934&radic&radic&radic S000006131SRP72YPL210CverifiedCore component of the signal recognition particle (SRP)ribonucleoprotein (RNP) complex that functionsin targeting nascent secretory proteins to theendoplasmic reticulum (ER) membrane 0.017830.27013&radic&radic&radic&radic&radic&radic&radic S000002461CDC34YDR054CverifiedUbiquitin-conjugating enzyme or E2; together with Skp1p, Rbx1p,Cdc53p, and an F-box protein, forms aubiquitin-protein ligase called the SCF complexwhich regulates cell cycle progression bytargeting key substrates for degradation 0.017790.26945&radic&radic&radic&radic&radic S000000259PRP6YBR055CverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.017720.26817&radic S000004283SEC72YLR292CverifiedNon-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66pand Sec72p); with Sec61 complex, Kar2p/BiP andLhs1p forms a channel competent forSRP-dependent and post-translationalSRP-independent protein targeting and importinto the ER 0.017470.26034&radic S000001616YKL133CUncharacterizedHypothetical protein 0.01730.2595&radic&radic&radic S000004107CLF1YLR117CverifiedEssential splicesome assembly factor; contains multipletetratricopeptide repeat (TPR) protein-bindingmotifs and interacts specifically with manyspliceosome components, may serve as a scaffoldduring splicesome assembly 0.01730.2595&radic&radic&radic&radic&radic S000005436MDM20YOL076WverifiedNon-catalytic subunit of the NatB N-terminal acetyltransferase,which catalyzes acetylation of theamino-terminal methionine residues of allproteins beginning with Met-Asp or Met-Glu andof some proteins beginning with Met-Asn orMet-Met; involved in mitochondrial inheritanceand actin assembly 0.01670.24776&radic&radic&radic&radic&radic S000002172NOP1YDL014WverifiedNucleolar protein, component of the small subunit processomecomplex, which is required for processing ofpre-18S rRNA; has similarity to mammalianfibrillarin 0.016580.24532 S000001427YIL165CUncharacterizedHypothetical protein 0.016570.24532&radic S000004689YMR084WUncharacterizedHypothetical protein 0.016360.24082 S000000656TAH1YCR060WverifiedHSP90 cofactor; interacts with Hsp82p, Pih1p, Rvb1 and Rvb2,contains a single TPR domain with at least twoTPR motifs 0.016170.23716&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p 0.016160.23614&radic&radic&radic S000001915FAB1YFR019Wverified1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membranekinase that generates phosphatidylinositol(3,5)P2, which is involved in vacuolar sortingand homeostasis 0.016070.2352&radic S000003105SEC27YGL137WverifiedEssential beta'-coat protein of the COPI coatomer, involved inER-to-Golgi and Golgi-to-ER transport; containsWD40 domains that mediate cargo selectiveinteractions; 45% sequence identity tomammalian beta'-COP 0.015880.23148 S000003355PPT1YGR123CverifiedProtein serine/threonine phosphatase with similarity to humanphosphatase PP5; present in both the nucleusand cytoplasm; expressed during logarithmicgrowth 0.015630.22495&radic&radic&radic&radic S000006312RPN7YPR108WverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome,similar to another S. cerevisiae regulatorysubunit, Rpn5p, as well as to mammalianproteasome subunits 0.015710.22495&radic&radic&radic&radic S000002568YDR161WUncharacterizedinteracts with PP2C 0.015660.22495&radic&radic&radic&radic&radic&radic S000003635CHS6YJL099WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p, from the Golgi to plasma membrane 0.015310.21866 S000004614CLU1YMR012WverifiedeIF3 component of unknown function; deletion causes defects inmitochondrial organization but not in growth ortranslation initiation, can rescue cytokinesisand mitochondrial organization defects of theDictyostelium cluA- mutant 0.014830.209&radic&radic&radic&radic S000002790RPP2BYDR382WverifiedRibosomal protein P2 beta, a component of the ribosomal stalk,which is involved in the interaction betweentranslational elongation factors and theribosome; regulates the accumulation of P1(Rpp1Ap and Rpp1Bp) in the cytoplasm 0.014810.20845&radic&radic&radic&radic S000001334HOP1YIL072WverifiedMeiosis-specific DNA binding protein that displays Red1pdependent localization to the unsynapsedaxial-lateral elements of the synaptonemalcomplex; required for homologous chromosomesynapsis and chiasma formation 0.014680.20585&radic&radic&radic&radic&radic S000000056BDH1YAL060WverifiedNAD-dependent (2R,3R)-2,3-butanediol dehydrogenase, azinc-containing medium-chain alcoholdehydrogenase, produces 2,3-butanediol fromacetoin during fermentation and allows using2,3-butanediol as a carbon source duringaerobic growth 0.014510.20074&radic&radic&radic&radic&radic S000004890FCP1YMR277WverifiedCarboxy-terminal domain (CTD) phosphatase, essential fordephosphorylation of the repeated C-terminaldomain of the RNA polymerase II large subunit(Rpo21p) 0.014430.20035&radic&radic&radic S000003430YPP1YGR198WverifiedCargo-transport protein involved in endocytosis; may interactwith ribosomes, based on co-purificationexperiments; GFP-fusion protein localizes tothe cytoplasm; YGR198W is an essential gene 0.014420.19957&radic&radic S000004092APC9YLR102CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.014380.19932&radic&radic&radic S000002668SWM1YDR260CverifiedSubunit of the anaphase-promoting complex, which is an E3ubiquitin ligase that regulates themetaphase-anaphase transition and exit frommitosis; required for activation of thedaughter-specific gene expression and sporewall maturation 0.014340.1977&radic&radic&radic&radic S000004376IKI3YLR384CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;maintains structural integrity of Elongator;homolog of human IKAP, mutations in which causefamilial dysautonomia (FD) 0.014240.19584&radic&radic&radic&radic S000001141TRA1YHR099WverifiedSubunit of SAGA and NuA4 histone acetyltransferase complexes;interacts with acidic activators (e.g., Gal4p)which leads to transcription activation;similar to human TRRAP, which is a cofactor forc-Myc mediated oncogenic transformation 0.014180.19495 S000000359CNS1YBR155WverifiedTPR-containing co-chaperone; binds both Hsp82p (Hsp90) andSsa1p (Hsp70) and stimulates the ATPaseactivity of SSA1, ts mutants reduce Hsp82pfunction while over expression suppresses thephenotypes of an HSP82 ts allele and a cpr7deletion 0.014170.19495&radic S000000461POP4YBR257WverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends; binds to the RPR1 RNAsubunit in Rnase P 0.014040.19302&radic&radic&radic&radic&radic S000003174CHC1YGL206CverifiedClathrin heavy chain, subunit of the major coat proteininvolved in intracellular protein transport andendocytosis; two heavy chains form the clathrintriskelion structural component; the lightchain (CLC1) is thought to regulate function 0.013960.19091&radic S000001220YHR177WUncharacterized 0.013820.18861&radic&radic&radic&radic S000003723SWE1YJL187CverifiedProtein kinase that regulates the G2/M transition by inhibitionof Cdc28p kinase activity; localizes to thenucleus and to the daughter side of themother-bud neck; homolog of S. pombe Wee1p;potential Cdc28p substrate 0.013820.18861&radic&radic S000004279MEC3YLR288CverifiedDNA damage and meiotic pachytene checkpoint protein; subunit ofa heterotrimeric complex (Rad17p-Mec3p-Ddc1p)that forms a sliding clamp, loaded onto partialduplex DNA by a clamp loader complex; homologof human and S. pombe Hus1 0.013560.18324&radic&radic&radic S000001464MND2YIR025WverifiedSubunit of the anaphase-promoting complex (APC); needed formeiotic nuclear division 0.013710.18324&radic&radic&radic&radic S000004700CTF13YMR094WverifiedSubunit of the CBF3 complex, which binds to the CDE III elementof centromeres, bending the DNA upon binding,and may be involved in sister chromatidcohesion during mitosis 0.013510.18324 S000001159TOM71YHR117WverifiedMitochondrial outer membrane protein with similarity to Tom70p;probable minor component of the TOM(translocase of outer membrane) complexresponsible for recognition and import ofmitochondrially directed proteins 0.01360.18324&radic&radic&radic&radic S000003793CPR7YJR032WverifiedPeptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzesthe cis-trans isomerization of peptide bondsN-terminal to proline residues; binds to Hsp82pand contributes to chaperone activity 0.013350.18133&radic&radic S000002736SKP1YDR328CverifiedEvolutionarily conserved kinetochore protein that is part ofmultiple protein complexes, including the SCFubiquitin ligase complex, the CBF3 complex thatbinds centromeric DNA, and the RAVE complexthat regulates assembly of the V-ATPase &radic0.013350.18133&radic&radic&radic&radic&radic S000002222UBC9YDL064WverifiedSUMO-conjugating enzyme involved in the Smt3p conjugationpathway; nuclear protein required for S- andM-phase cyclin degradation and mitotic control;involved in proteolysis mediated by theanaphase-promoting complex cyclosome (APCC) 0.013320.17912&radic&radic S000005065TOM70YNL121CverifiedComponent of the TOM (translocase of outer membrane) complexresponsible for recognition and initial importsteps for all mitochondrially directedproteins; acts as a receptor for incomingprecursor proteins 0.013280.17912 S000002265MSS2YDL107WverifiedPeripherally bound inner membrane protein of the mitochondrialmatrix, required for export of C-terminal tailof Cox2p through the inner membrane 0.012940.17274&radic S000003567MAD2YJL030WverifiedComponent of the spindle-assembly checkpoint complex, whichdelays the onset of anaphase in cells withdefects in mitotic spindle assembly; forms acomplex with Mad1p 0.012830.17274&radic S000005505CTR9YOL145CverifiedComponent of the Paf1p complex, which is a large complex thatbinds to and modulates the activity of RNApolymerase II and is required for expression ofa subset of genes, including cyclin genes;contains TPR repeats 0.012810.17253 S000003848YJR088CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum 0.012770.17186&radic S000004497SRC1YML034WverifiedProtein with a putative role in sister chromatid segregation,potentially phosphorylated by Cdc28p; greenfluorescent protein (GFP)-fusion proteinlocalizes to the nuclear periphery 0.012780.17186&radic&radic&radic&radic&radic&radic S000004798HSC82YMR186WverifiedCytoplasmic chaperone of the Hsp90 family, redundant infunction and nearly identical with Hsp82p, andtogether they are essential; expressedconstitutively at 10-fold higher basal levelsthat HSP82 and induced 2-3 fold by heat shock 0.012630.16967&radic&radic&radic&radic&radic S000000146SEC17YBL050WverifiedPeripheral membrane protein required for vesicular transportbetween ER and Golgi and for the 'priming' stepin homotypic vacuole fusion, part of thecis-SNARE complex; has similarity to alpha-SNAP 0.01260.16765&radic&radic&radic S000001750UTH1YKR042WverifiedMitochondrial outer membrane and cell wall localized SUN familymember required for mitochondrial autophagy;involved in the oxidative stress response, lifespan during starvation, mitochondrialbiogenesis, and cell death 0.012480.16706&radic&radic&radic S000000456DUT1YBR252WverifieddUTPase, catalyzes hydrolysis of dUTP to dUMP and PPi, therebypreventing incorporation of uracil into DNAduring replication; critical for themaintenance of genetic stability 0.012380.16553&radic&radic&radic&radic S000000763RAD23YEL037CverifiedProtein with ubiquitin-like N terminus, recognizes and bindsdamaged DNA (with Rad4p) during nucleotideexcision repair; regulates Rad4p levels,subunit of Nuclear Excision Repair Factor 2(NEF2); homolog of human HR23A and HR23Bproteins 0.012290.16459&radic&radic&radic&radic S000001932CDC26YFR036WverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.012140.16263&radic&radic&radic&radic&radic S000001338SEC28YIL076WverifiedEpsilon-COP subunit of the coatomer; regulates retrogradeGolgi-to-ER protein traffic; stabilizes Cop1p,the alpha-COP and the coatomer complex;non-essential for cell growth 0.012190.16263&radic&radic&radic&radic S000001562SMY1YKL079WverifiedProtein that interacts with Myo2p, proposed to be involved inexocytosis; N-terminal domain is related to themotor domain of kinesins 0.012210.16263&radic&radic&radic&radic S000003667YJL131CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria; YJL131C is a non-essential gene 0.012070.16127&radic&radic&radic&radic&radic S000002330GLT1YDL171CverifiedNAD(+)-dependent glutamate synthase (GOGAT), synthesizesglutamate from glutamine andalpha-ketoglutarate; with Gln1p, forms thesecondary pathway for glutamate biosynthesisfrom ammonia; expression regulated by nitrogensource 0.011830.15745&radic S000000062SEO1YAL067CverifiedPutative permease, member of the allantoate transportersubfamily of the major facilitator superfamily;mutation confers resistance to ethioninesulfoxide 0.011820.15745&radic&radic&radic&radic&radic S000005999ATP4YPL078CverifiedSubunit b of the stator stalk of mitochondrial F1F0 ATPsynthase, which is a large, evolutionarilyconserved enzyme complex required for ATPsynthesis 0.011880.15745&radic&radic S000002438MIC14YDR031WverifiedMitochondrial intermembrane space cysteine motif protein of 14kDa 0.011870.15745&radic S000002540YDR133CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C 0.011890.15745&radic&radic&radic&radic&radic S000005228MRPL10YNL284CverifiedMitochondrial ribosomal protein of the large subunit; appearsas two protein spots (YmL10 and YmL18) ontwo-dimensional SDS gels 0.011860.15745 S000003073ARC1YGL105WverifiedProtein that binds tRNA and methionyl- and glutamyl-tRNAsynthetases (Mes1p and Gus1p), delivering tRNAto them, stimulating catalysis, and ensuringtheir localization to the cytoplasm; also bindsquadruplex nucleic acids 0.011780.15534&radic S000001817YAL065CUncharacterizedHypothetical protein 0.011520.15251&radic&radic&radic&radic S000003717YJL181WUncharacterizedHypothetical protein 0.011510.15149&radic&radic&radic&radic&radic S000005784HNT3YOR258WverifiedMember of the third branch of the histidine triad (HIT)superfamily of nucleotide-binding proteins;similar to Aprataxin, a Hint related proteinthat is mutated in individuals with ataxia withoculomotor apraxia 0.011490.15149&radic&radic&radic S000000119MCM2YBL023CverifiedProtein involved in DNA replication; component of the Mcm2-7hexameric complex that binds chromatin as apart of the pre-replicative complex 0.011430.15093&radic&radic&radic&radic S000000252RPS11BYBR048WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps11Ap and has similarity to E.coli S17 and rat S11 ribosomal proteins 0.011310.14889&radic S000002971CDH1YGL003CverifiedCell-cycle regulated activator of the anaphase-promotingcomplex/cyclosome (APC/C), which directsubiquitination of mitotic cyclins resulting inexit from mitosis; targets the APC/C tospecific substrates including CDC20, ASE1 andCIN8 0.011330.14889&radic S000002796RVS167YDR388WverifiedActin-associated protein, subunit of a complex(Rvs161p-Rvs167p) involved in regulation ofactin cytoskeleton, endocytosis, and viabilityfollowing starvation or osmotic stress; homologof mammalian amphiphysin 0.011270.14829&radic&radic&radic S000005427RIB2YOL066CverifiedDRAP deaminase, catalyzes the third step of the riboflavinbiosynthesis pathway; cytoplasmic tRNApseudouridine synthase involved inpseudouridylation of cytoplasmic tRNAs atposition 32 0.011140.14586&radic&radic&radic&radic&radic&radic S000006005BRO1YPL084WverifiedCytoplasmic class E vacuolar protein sorting (VPS) factor thatcoordinates deubiquitination in themultivesicular body (MVB) pathway by recruitingDoa4p to endosomes 0.011160.14586&radic&radic&radic&radic&radic S000005777YOR251CUncharacterizedcatalyzes transfer of the sulfane atom of thiosulfate to cyanide to form sulfite and thiocyanate 0.011190.14586&radic&radic&radic&radic&radic&radic S000003058LIF1YGL090WverifiedProtein involved in DNA double-strand break repair; physicallyinteracts with DNA ligase 4 (Lig4p); homologousto mammalian XRCC4 protein 0.011080.14474&radic&radic&radic&radic S000006161HSP82YPL240CverifiedCytoplasmic chaperone (Hsp90 family) required for pheromonesignaling and negative regulation of Hsf1p;docks with the mitochondrial import receptorTom70p for preprotein delivery; interacts withco-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p 0.010960.14208&radic&radic&radic&radic&radic S000001005OTU2YHL013CverifiedProtein of unknown function that may interact with ribosomes,based on co-purification experiments; member ofthe ovarian tumor-like (OTU) superfamily ofpredicted cysteine proteases; shows cytoplasmiclocalization 0.0110.14208&radic&radic&radic&radic S000000137PRE7YBL041Wverified20S proteasome beta-type subunit 0.011050.14208&radic S000001751YKR043CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.010880.14043&radic&radic&radic&radic&radic&radic S000002483RAD55YDR076WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad57p 0.010790.13669&radic&radic&radic&radic S000004665RNA14YMR061WverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; bridges interaction between Rna15p andHrp1p in the CF I complex 0.010690.13665 S000006177CLN2YPL256CverifiedG1 cyclin involved in regulation of the cell cycle; activatesCdc28p kinase to promote the G1 to S phasetransition; late G1 specific expression dependson transcription factor complexes, MBF(Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) 0.010640.13449&radic&radic&radic&radic&radic S000006168YPL247CUncharacterizedHypothetical protein 0.010570.13325&radic&radic&radic&radic&radic S000002520PDS1YDR113CverifiedSecurin that inhibits anaphase by binding separin Esp1p, alsoblocks cyclin destruction and mitotic exit,essential for cell cycle arrest in mitosis inthe presence of DNA damage or aberrant mitoticspindles; also present in meiotic nuclei 0.010470.12963&radic&radic&radic&radic&radic S000001791DAD2YKR083CverifiedEssential subunit of the Dam1 complex (aka DASH complex),couples kinetochores to the force produced byMT depolymerization thereby aiding inchromosome segregation; is transferred to thekinetochore prior to mitosis 0.010440.12963&radic&radic&radic&radic&radic S000001664PRS1YKL181Wverified5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involvedin nucleotide, histidine, and tryptophanbiosynthesis; one of five related enzymes,which are active as heteromultimeric complexes 0.010490.12963&radic&radic&radic&radic S000002494RRP1YDR087CverifiedEssential evolutionarily conserved nucleolar protein necessaryfor biogenesis of 60S ribosomal subunits andprocessing of pre-rRNAs to mature rRNAs,associated with several distinct 66Spre-ribosomal particles 0.010360.12783&radic&radic&radic&radic S000001735BCH2YKR027WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p, from the Golgi to plasma membrane 0.010070.12069 S000001149CDC12YHR107CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM 0.010070.12069&radic&radic&radic&radic S000003472PFK1YGR240CverifiedAlpha subunit of heterooctameric phosphofructokinase involvedin glycolysis, indispensable for anaerobicgrowth, activated by fructose-2,6-bisphosphateand AMP, mutation inhibits glucose induction ofcell cycle-related genes 0.010050.12027&radic&radic&radic&radic&radic&radic S000004214YLR224WUncharacterizedF-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene 0.009970.11869&radic&radic&radic&radic&radic&radic S000000339CKS1YBR135WverifiedSubunit of the Cdc28 protein kinase, required for mitoticproteolysis, may also be involved in theproteolysis of the G1 cyclins 0.009880.11664&radic&radic S000001746KAE1YKR038CverifiedPutative glycoprotease proposed to be in transcription as acomponent of the EKC protein complex withBud32p, Cgi121p, Pcc1p, and Gon7p; alsoidentified as a component of the KEOPS proteincomplex 0.009910.11664&radic&radic&radic S000001337RPN2YIL075CverifiedSubunit of the 26S proteasome, substrate of theN-acetyltransferase Nat1p 0.009840.11599&radic&radic S000003272KSS1YGR040WverifiedMitogen-activated protein kinase (MAPK) involved in signaltransduction pathways that control filamentousgrowth and pheromone response 0.009780.11463&radic&radic S000003446RPS0AYGR214WverifiedProtein component of the small (40S) ribosomal subunit, nearlyidentical to Rps0Bp; required for maturation of18S rRNA along with Rps0Bp; deletion of eitherRPS0 gene reduces growth rate, deletion of bothgenes is lethal 0.009650.11235&radic&radic S000003026RAD6YGL058WverifiedUbiquitin-conjugating enzyme (E2), involved in postreplicationrepair (with Rad18p), sporulation, telomeresilencing, and ubiquitin-mediated N-end ruleprotein degradation (with Ubr1p) 0.009660.11235&radic&radic&radic S000004165CBF5YLR175WverifiedPseudouridine synthase catalytic subunit of box H/ACA smallnucleolar ribonucleoprotein particles(snoRNPs), acts on both large and small rRNAsand on snRNA U2 0.00970.11235&radic&radic&radic&radic&radic&radic S000003223FZF1YGL254WverifiedTranscription factor involved in sulfite metabolism, soleidentified regulatory target is SSU1,overexpression suppresses sulfite-sensitivityof many unrelated mutants due tohyperactivation of SSU1, contains five zincfingers 0.009640.11235&radic&radic&radic&radic&radic S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus 0.009550.11047&radic&radic&radic S000002285PCL2YDL127WverifiedG1 cyclin, associates with Pho85p cyclin-dependent kinase (Cdk)to contribute to entry into the mitotic cellcycle, essential for cell morphogenesis;localizes to sites of polarized cell growth 0.009430.10887&radic S000003016RPT6YGL048CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; bound byubiquitin-protein ligases Ubr1p and Ufd4p;localized mainly to the nucleus throughout thecell cycle 0.009410.10851&radic&radic S000001410RPL40AYIL148WverifiedFusion protein, identical to Rpl40Bp, that is cleaved to yieldubiquitin and a ribosomal protein of the large(60S) ribosomal subunit with similarity to ratL40; ubiquitin may facilitate assembly of theribosomal protein into ribosomes 0.009420.10851&radic S000002299RPO21YDL140CverifiedRNA polymerase II largest subunit B220, part of central core;phosphorylation of C-terminal heptapeptiderepeat domain regulates association withtranscription and splicing factors; similar tobacterial beta-prime 0.009390.1082 S000003176SIP2YGL208WverifiedOne of three beta subunits of the Snf1 serine/threonine proteinkinase complex involved in the response toglucose starvation; null mutants exhibitaccelerated aging; N-myristoylprotein localizedto the cytoplasm and the plasma membrane 0.009380.1082&radic&radic&radic&radic&radic S000005650UBP2YOR124CverifiedUbiquitin-specific protease that removes ubiquitin fromubiquitinated proteins, cleaves at the Cterminus of ubiquitin fusions; capable ofcleaving polyubiquitin and possessesisopeptidase activity 0.009380.1082&radic&radic&radic S000006314RPC40YPR110CverifiedRNA polymerase subunit, common to RNA polymerase I and III 0.009330.10607&radic&radic S000000381EHT1YBR177CverifiedAcyl-coenzymeA:ethanol O-acyltransferase that plays a minorrole in medium-chain fatty acid ethyl esterbiosynthesis; contains esterase activity;localizes to lipid particles and themitochondrial outer membrane 0.009290.10607&radic&radic&radic&radic S000001069RPN1YHR027CverifiedNon-ATPase base subunit of the 19S regulatory particle of the26S proteasome; may participate in therecognition of several ligands of theproteasome; contains a leucine-rich repeat(LRR) domain, a site for protein?proteininteractions 0.009290.10607&radic&radic&radic S000006323CLB2YPR119WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 0.009310.10607&radic&radic S000004624QRI8YMR022WverifiedUbiquitin conjugating enzyme, involved in the ER-associatedprotein degradation pathway; requires Cue1p forrecruitment to the ER membrane; proposed to beinvolved in chromatin assembly 0.009250.10607&radic&radic&radic&radic S000000814PRE1YER012Wverified20S proteasome beta-type subunit; localizes to the nucleusthroughout the cell cycle 0.009240.10607&radic&radic&radic&radic S000001049ERG11YHR007CverifiedLanosterol 14-alpha-demethylase, catalyzes the C-14demethylation of lanosterol to form4,4''-dimethylcholesta-8,14,24-triene-3-beta-ol in theergosterol biosynthesis pathway; member of thecytochrome P450 family 0.009350.10607&radic&radic&radic&radic&radic&radic&radic&radic S000002794MUS81YDR386WverifiedHelix-hairpin-helix protein, involved in DNA repair andreplication fork stability; functions as anendonuclease in complex with Mms4p; interactswith Rad54p 0.009210.10538&radic&radic&radic S000002525APC4YDR118WverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.009180.10538&radic&radic&radic&radic&radic S000004603CDC5YMR001CverifiedPolo-like kinase with similarity to Xenopus Plx1 and S. pombePlo1p; found at bud neck, nucleus and SPBs; hasmultiple functions in mitosis and cytokinesisthrough phosphorylation of substrates; may be aCdc28p substrate 0.009140.10462&radic&radic&radic&radic S000004170SAM1YLR180WverifiedS-adenosylmethionine synthetase, catalyzes transfer of theadenosyl group of ATP to the sulfur atom ofmethionine; one of two differentially regulatedisozymes (Sam1p and Sam2p) 0.009150.10462&radic&radic&radic&radic S000004096MDN1YLR106CverifiedHuge dynein-related AAA-type ATPase (midasin), forms extendedpre-60S particle with the Rix1 complex(Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependentremodeling of 60S subunits and subsequentexport from nucleoplasm to cytoplasm 0.009130.10462&radic&radic&radic&radic S000004975HHF2YNL030WverifiedOne of two identical histone H4 proteins (see also HHF1); corehistone required for chromatin assembly andchromosome function; contributes to telomericsilencing; N-terminal domain involved inmaintaining genomic integrity 0.009130.10462 S000004294MET17YLR303WverifiedO-acetyl homoserine-O-acetyl serine sulfhydrylase, required forsulfur amino acid synthesis 0.009070.10323&radic&radic&radic&radic S000005868RPA190YOR341WverifiedRNA polymerase I subunit; largest subunit of RNA polymerase I 0.009050.10323&radic&radic S000001052RPL27AYHR010WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl27Bp and has similarity to ratL27 ribosomal protein 0.009070.10323&radic&radic&radic&radic&radic S000001734GCN3YKR026CverifiedAlpha subunit of the translation initiation factor eIF2B, theguanine-nucleotide exchange factor for eIF2;activity subsequently regulated byphosphorylated eIF2; first identified as apositive regulator of GCN4 expression 0.008930.10155&radic&radic&radic&radic S000002885SNF1YDR477WverifiedAMP-activated serine/threonine protein kinase found in acomplex containing Snf4p and members of theSip1p/Sip2p/Gal83p family; required fortranscription of glucose-repressed genes,thermotolerance, sporulation, and peroxisomebiogenesis 0.008910.10155&radic S000002904PUF6YDR496CverifiedPumilio-homology domain protein that binds ASH1 mRNA at PUFconsensus sequences in the 3' UTR and repressesits translation, resulting in proper asymmetriclocalization of ASH1 mRNA 0.008890.10155 S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.008880.10137&radic S000002625RAD9YDR217CverifiedDNA damage-dependent checkpoint protein, required forcell-cycle arrest in G1/S, intra-S, and G2/M;transmits checkpoint signal by activatingRad53p and Chk1p; hyperphosphorylated by Mec1pand Tel1p; potential Cdc28p substrate 0.008870.10137&radic&radic&radic&radic S000004926NIP1YMR309CverifiedSubunit of the eukaryotic translation initiation factor 3(eIF3), involved in the assembly ofpreinitiation complex and start codon selection 0.008850.10113&radic&radic&radic S000001512MAE1YKL029CverifiedMitochondrial malic enzyme, catalyzes the oxidativedecarboxylation of malate to pyruvate, which isa key intermediate in sugar metabolism and aprecursor for synthesis of several amino acids 0.008860.10113&radic&radic&radic&radic S000001444IST3YIR005WverifiedComponent of the U2 snRNP, required for the first catalyticstep of splicing and for spliceosomal assembly;interacts with Rds3p and is required forMer1p-activated splicing 0.008830.101&radic&radic S000002764SPC110YDR356WverifiedInner plaque spindle pole body (SPB) component, ortholog ofhuman kendrin; involved in connecting nuclearmicrotubules to SPB; interacts withTub4p-complex and calmodulin; phosphorylated byMps1p in cell cycle-dependent manner 0.008820.10078&radic&radic&radic S000003084CDC20YGL116WverifiedCell-cycle regulated activator of anaphase-promotingcomplex/cyclosome (APC/C), which is requiredfor metaphase/anaphase transition; directsubiquitination of mitotic cyclins, Pds1p, andother anaphase inhibitors; potential Cdc28psubstrate 0.008820.10078&radic S000001249SKN7YHR206WverifiedNuclear response regulator and transcription factor, part of abranched two-component signaling system;required for optimal induction of heat-shockgenes in response to oxidative stress; involvedin osmoregulation 0.008790.10053&radic&radic&radic&radic&radic S000000494BSD2YBR290WverifiedHeavy metal ion homeostasis protein, facilitates trafficking ofSmf1p and Smf2p metal transporters to thevacuole where they are degraded, controls metalion transport, prevents metalhyperaccumulation, functions in copperdetoxification 0.008750.09996&radic S000005398PRE6YOL038Wverified20S proteasome alpha-type subunit; green fluorescent protein(GFP)-fusion protein relocates from the cytosolto the mitochondrial outer surface uponoxidative stress 0.008780.09996&radic&radic&radic&radic&radic&radic S000004888BUL1YMR275CverifiedUbiquitin-binding component of the Rsp5p E3-ubiquitin ligasecomplex, functional homolog of Bul2p,disruption causes temperature-sensitive growth,overexpression causes missorting of amino acidpermeases 0.008770.09996&radic&radic&radic&radic S000003083SNF4YGL115WverifiedProtein kinase activator found in a complex containing Snf1pand members of the Sip1p/Sip2p/Gal83p family;activates the Snf1p protein kinase; involved inexpression of glucose-repressed genes,sporulation, and peroxisome biogenesis 0.008740.09996&radic&radic S000004239YEF3YLR249WverifiedTranslational elongation factor, stimulates the binding ofaminoacyl-tRNA (AA-tRNA) to ribosomes byreleasing EF-1 alpha from the ribosomalcomplex; contains two ABC cassettes; binds andhydrolyses ATP 0.008760.09996&radic&radic&radic&radic&radic S000003962UBI4YLL039CverifiedUbiquitin, becomes conjugated to proteins, marking them forselective degradation via the ubiquitin-26Sproteasome system; essential for the cellularstress response 0.008720.09974&radic&radic&radic&radic&radic&radic S000003794RAV1YJR033CverifiedSubunit of the RAVE complex (Rav1p, Rav2p, Skp1p), whichpromotes assembly of the V-ATPase holoenzyme;required for transport between the early andlate endosome/PVC and for localization of TGNmembrane proteins; potential Cdc28p substrate 0.008690.09947&radic S000005853MYO2YOR326WverifiedOne of two type V myosin motors (along with MYO4) involved inactin-based transport of cargos; required forthe polarized delivery of secretory vesicles,the vacuole, late Golgi elements, peroxisomes,and the mitotic spindle 0.00870.09947&radic&radic&radic&radic S000000682CSM1YCR086WverifiedNucleolar protein that forms a complex with Lrs4p which bindsMam1p at kinetochores during meiosis I tomediate accurate chromosome segregation, may beinvolved in premeiotic DNA replication;possible role in telomere maintenance 0.008650.09889&radic&radic S000004329VRP1YLR337CverifiedProline-rich actin-associated protein involved in cytoskeletalorganization and cytokinesis; related tomammalian Wiskott-Aldrich syndrome protein(WASP)-interacting protein (WIP) 0.008650.09889&radic&radic&radic&radic&radic S000000981DMC1YER179WverifiedMeiosis-specific protein required for repair of double-strandbreaks and pairing between homologouschromosomes; homolog of Rad51p and thebacterial RecA protein 0.008640.09889 S000004069SIC1YLR079WverifiedInhibitor of Cdc28-Clb kinase complexes that controls G1/Sphase transition, preventing premature S phaseand ensuring genomic integrity; phosphorylationtargets Sic1p for SCF(CDC4)-dependent turnover;functional homolog of mammalian Kip1 0.008610.09855&radic&radic&radic S000000331VMA2YBR127CverifiedSubunit B of the eight-subunit V1 peripheral membrane domain ofthe vacuolar H+-ATPase (V-ATPase), anelectrogenic proton pump found throughout theendomembrane system; contains nucleotidebinding sites; also detected in the cytoplasm 0.008610.09855&radic S000005887PDE2YOR360CverifiedHigh-affinity cyclic AMP phosphodiesterase, component of thecAMP-dependent protein kinase signaling system,protects the cell from extracellular cAMP,contains readthrough motif surroundingtermination codon 0.008580.09806&radic&radic&radic&radic&radic S000006317PIS1YPR113WverifiedPhosphatidylinositol synthase, required for biosynthesis ofphosphatidylinositol, which is a precursor forpolyphosphoinositides, sphingolipids, andglycolipid anchors for some of the plasmamembrane proteins 0.008590.09806&radic S000006214RPA135YPR010CverifiedRNA polymerase I subunit A135 0.008550.09762 S000003163GCN1YGL195WverifiedPositive regulator of the Gcn2p kinase activity, forms acomplex with Gcn20p; proposed to stimulateGcn2p activation by an uncharged tRNA 0.008540.09762&radic&radic S000004442HMG2YLR450WverifiedOne of two isozymes of HMG-CoA reductase that convert HMG-CoAto mevalonate, a rate-limiting step in sterolbiosynthesis; overproduction induces assemblyof peripheral ER membrane arrays and shortnuclear-associated membrane stacks 0.008550.09762 S000004778CEP3YMR168CverifiedEssential kinetochore protein, component of the CBF3 complexthat binds the CDEIII region of the centromere;contains an N-terminal Zn2Cys6 type zinc fingerdomain, a C-terminal acidic domain, and aputative coiled coil dimerization domain 0.008530.09762&radic&radic S000001726YKR018CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.008540.09762&radic&radic S000001767TIF1YKR059WverifiedTranslation initiation factor eIF4A, identical to Tif2p;DEA(D/H)-box RNA helicase that couples ATPaseactivity to RNA binding and unwinding; forms adumbbell structure of two compact domainsconnected by a linker; interacts with eIF4G 0.008560.09762&radic&radic&radic&radic S000004852RNT1YMR239CverifiedRNAase III; cleaves a stem-loop structure at the 3' end of U2snRNA to ensure formation of the correct U2 3'end 0.008540.09762&radic&radic&radic&radic S000004712YKU80YMR106CverifiedSubunit of the telomeric Ku complex (Yku70p-Yku80p), involvedin telomere length maintenance, structure andtelomere position effect; relocates to sites ofdouble-strand cleavage to promote nonhomologousend joining during DSB repair 0.008480.09587&radic S000002610RAV2YDR202CverifiedSubunit of RAVE (Rav1p, Rav2p, Skp1p), a complex thatassociates with the V1 domain of the vacuolarmembrane (H+)-ATPase (V-ATPase) and promotesassembly and reassembly of the holoenzyme 0.008510.09587&radic&radic S000005659EFT1YOR133WverifiedElongation factor 2 (EF-2), also encoded by EFT2; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 0.008440.09587&radic&radic&radic&radic S000004663SEN15YMR059WverifiedSubunit of the tRNA splicing endonuclease, which is composed ofSen2p, Sen15p, Sen34p, and Sen54p 0.008440.09587 S000003450CRM1YGR218WverifiedMajor karyopherin, involved in export of proteins, RNAs, andribosomal subunits from the nucleus 0.008460.09587&radic&radic S000003516ERV29YGR284CverifiedProtein localized to COPII-coated vesicles, involved in vesicleformation and incorporation of specificsecretory cargo 0.008430.09587&radic&radic&radic&radic S000003884RPS5YJR123WverifiedProtein component of the small (40S) ribosomal subunit, theleast basic of the non-acidic ribosomalproteins; phosphorylated in vivo; essential forviability; has similarity to E. coli S7 and ratS5 ribosomal proteins 0.008440.09587&radic&radic S000005454RFC4YOL094CverifiedSubunit of heteropentameric Replication factor C (RF-C), whichis a DNA binding protein and ATPase that actsas a clamp loader of the proliferating cellnuclear antigen (PCNA) processivity factor forDNA polymerases delta and epsilon 0.008460.09587 S000003175SPT16YGL207WverifiedSubunit of the heterodimeric FACT complex (Spt16p-Pob3p),facilitates RNA Polymerase II transcriptionelongation through nucleosomes by destabilizingand then reassembling nucleosome structure 0.00850.09587&radic&radic&radic&radic S000005204YNL260CUncharacterizedPutative protein of unknown function with similarity to a human protein overexpressed in oral cancers; localizes to the nucleus and cytoplasm; YNL260C is an essential gene 0.008470.09587&radic S000006307PRE2YPR103Wverified20S proteasome beta-type subunit, responsible for thechymotryptic activity of the proteasome 0.008430.09587&radic S000000313CMD1YBR109CverifiedCalmodulin; Ca++ binding protein that regulates Ca++independent processes (mitosis, bud growth,actin organization, endocytosis, etc.) and Ca++dependent processes (stress-activatedpathways), targets include Nuf1p, Myo2p andcalcineurin 0.008490.09587&radic S000002213PSA1YDL055CverifiedGDP-mannose pyrophosphorylase (mannose-1-phosphateguanyltransferase), synthesizes GDP-mannosefrom GTP and mannose-1-phosphate in cell wallbiosynthesis; required for normal cell wallstructure 0.008520.09587&radic&radic&radic S000004540HMG1YML075CverifiedOne of two isozymes of HMG-CoA reductase that catalyzes theconversion of HMG-CoA to mevalonate, which is arate-limiting step in sterol biosynthesis;localizes to the nuclear envelope;overproduction induces the formation ofkarmellae 0.008320.0944 S000006156RVB2YPL235WverifiedEssential protein involved in transcription regulation;component of chromatin remodeling complexes;required for assembly and function of the INO80complex; member of the RUVB-like protein family 0.008340.0944&radic&radic&radic S000003407ERG1YGR175CverifiedSqualene epoxidase, catalyzes the epoxidation of squalene to2,3-oxidosqualene; plays an essential role inthe ergosterol-biosynthesis pathway and is thespecific target of the antifungal drugterbinafine 0.008320.0944&radic&radic&radic S000002205SIT4YDL047WverifiedType 2A-related serine-threonine phosphatase that functions inthe G1/S transition of the mitotic cycle;cytoplasmic and nuclear protein that modulatesfunctions mediated by Pkc1p including cell walland actin cytoskeleton organization 0.008380.0944&radic&radic S000003334YGR102CUncharacterizedPutative protein of unknown function; transposon insertion mutant is salt sensitive, and the systematic deletion mutant has growth defects; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion 0.008340.0944&radic&radic&radic S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.008380.0944&radic S000001325YRB2YIL063CverifiedProtein of unknown function involved in nuclear processes ofthe Ran-GTPase cycle; involved in nuclearprotein export; contains Ran Binding Domain andFxFG repeats; interacts with Srm1p, GTP-Gsp1p,Rna1p and Crm1p; is not essential 0.008370.0944&radic&radic&radic&radic&radic S000000058GDH3YAL062WverifiedNADP(+)-dependent glutamate dehydrogenase, synthesizesglutamate from ammonia and alpha-ketoglutarate;rate of alpha-ketoglutarate utilization differsfrom Gdh1p; expression regulated by nitrogenand carbon sources 0.008370.0944&radic&radic&radic S000004529TEM1YML064CverifiedGTP-binding protein of the ras superfamily involved intermination of M-phase; controls actomyosin andseptin dynamics during cytokinesis 0.008290.0944 S000000888ILV1YER086WverifiedThreonine deaminase, catalyzes the first step in isoleucinebiosynthesis; expression is under general aminoacid control; ILV1 locus exhibits highlypositioned nucleosomes whose organization isindependent of known ILV1 regulation 0.008320.0944 S000002506BMH2YDR099Wverified14-3-3 protein, minor isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.008250.09409&radic&radic&radic&radic S000000760HYP2YEL034WverifiedTranslation initiation factor eIF-5A, promotes formation of thefirst peptide bond; similar to and functionallyredundant with Anb1p; possible role intranslation elongation; undergoes an essentialhypusination modification 0.008250.09409&radic S000002435REG1YDR028CverifiedRegulatory subunit of type 1 protein phosphatase Glc7p,involved in negative regulation ofglucose-repressible genes 0.008240.09278&radic&radic S000001335SPO22YIL073CverifiedMeiosis-specific protein essential for chromosome synapsis,similar to phospholipase A2, involved incompletion of nuclear divisions during meiosis;induced early in meiosis 0.008210.09278&radic S000004280GUF1YLR289WverifiedMitochondrial GTPase of unknown function, similar to E. colielongation factor-type GTP-binding protein LepAand to LK1236.1 from Caenorhabditis elegans 0.008240.09278&radic&radic&radic&radic&radic&radic S000005309SEC12YNR026CverifiedGuanine nucleotide exchange factor (GEF); glycosylated integralmembrane protein of the endoplasmic reticulum,important for the initiation of transportvesicle budding from the endoplasmic reticulumthrough activation of the GTPase Sar1p 0.008230.09278&radic S000001333PCI8YIL071CverifiedPossible shared subunit of Cop9 signalosome (CSN) and eIF3,binds eIF3b subunit Prt1p, has possible dualfunctions in transcriptional and translationalcontrol, contains a PCI (Proteasome-COP9signalosome (CSN)-eIF3) domain 0.008150.09278&radic&radic&radic&radic&radic S000001356LYS12YIL094CverifiedHomo-isocitrate dehydrogenase, an NAD-linked mitochondrialenzyme required for the fourth step in thebiosynthesis of lysine, in whichhomo-isocitrate is oxidatively decarboxylatedto alpha-ketoadipate 0.008210.09278 S000002567SSY1YDR160WverifiedComponent of the SPS plasma membrane amino acid sensor system(Ssy1p-Ptr3p-Ssy5p), which senses externalamino acid concentration and transmitsintracellular signals that result in regulationof expression of amino acid permease genes 0.008210.09278&radic&radic&radic S000005188SUI1YNL244CverifiedTranslation initiation factor eIF1; component of a complexinvolved in recognition of the initiator codon;modulates translation accuracy at theinitiation phase 0.008190.09278&radic S000002482PPH3YDR075WverifiedCatalytic subunit of an evolutionarily conserved proteinphosphatase complex containing Psy2p and theregulatory subunit Psy4p; required forcisplatin resistance; involved in activation ofGln3p 0.008210.09278&radic&radic&radic&radic S000004494RAD52YML032CverifiedProtein that stimulates strand exchange by facilitating Rad51pbinding to single-stranded DNA; annealscomplementary single-stranded DNA; involved inthe repair of double-strand breaks in DNAduring vegetative growth and meiosis 0.008160.09278&radic&radic&radic S000000213HHF1YBR009CverifiedOne of two identical histone H4 proteins (see also HHF2); corehistone required for chromatin assembly andchromosome function; contributes to telomericsilencing; N-terminal domain involved inmaintaining genomic integrity 0.008220.09278 S000004384YLR392CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR392C is not an essential gene 0.008140.0925&radic&radic&radic&radic&radic S000006284TEF1YPR080WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF2; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.008140.0925&radic&radic S000002133PTC6YCR079WverifiedPhosphoprotein phosphatase type 2C; contains mitochondrialtargeting sequence; green fluorescent protein(GFP)-fusion protein localizes to themitochondrion; null mutant is sensitive torapamycin 0.008130.0925 S000002187ARP2YDL029WverifiedEssential component of the Arp2/3 complex, which is a highlyconserved actin nucleation center required forthe motility and integrity of actin patches;involved in endocytosis and membrane growth andpolarity 0.008080.09171&radic S000002104RPL2AYFR031C-AverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl2Bp and has similarity to E.coli L2 and rat L8 ribosomal proteins 0.008090.09171&radic&radic&radic&radic S000000330TPS1YBR126CverifiedSynthase subunit of trehalose-6-phosphate synthase/phosphatasecomplex, which synthesizes the storagecarbohydrate trehalose; also found in amonomeric form; expression is induced by thestress response and repressed by the Ras-cAMPpathway 0.00810.09171&radic&radic&radic S000005664RUP1YOR138CverifiedProtein involved in regulation of Rsp5p, which is an essentialHECT ubiquitin ligase; required for binding ofRsp5p to Ubp2p; contains an UBA domain 0.008110.09171&radic&radic&radic&radic&radic S000005480RPL18AYOL120CverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl18Bp and has similarity to ratL18 ribosomal protein; intron of RPL18Apre-mRNA forms stem-loop structures that are atarget for Rnt1p cleavage leading todegradation 0.008120.09171&radic S000000316CYC8YBR112CverifiedGeneral transcriptional co-repressor, acts together with Tup1p;also acts as part of a transcriptionalco-activator complex that recruits the SWI/SNFand SAGA complexes to promoters 0.00810.09171 S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 0.008130.09171&radic&radic&radic S000003131RAD54YGL163CverifiedDNA-dependent ATPase, stimulates strand exchange by modifyingthe topology of double-stranded DNA; involvedin the recombinational repair of double-strandbreaks in DNA during vegetative growth andmeiosis; member of the SWI/SNF family 0.008080.09126&radic S000001925PTR3YFR029WverifiedComponent of the SPS plasma membrane amino acid sensor system(Ssy1p-Ptr3p-Ssy5p), which senses externalamino acid concentration and transmitsintracellular signals that result in regulationof expression of amino acid permease genes 0.008050.09126 S000001930PHO4YFR034CverifiedBasic helix-loop-helix (bHLH) transcription factor; bindscooperatively with Pho2p to the PHO5 promoter;function is regulated by phosphorylation atmultiple sites and by phosphate availability 0.008070.09126&radic&radic&radic&radic&radic&radic&radic S000000286UBC4YBR082CverifiedUbiquitin-conjugating enzyme that mediates degradation ofshort-lived and abnormal proteins; interactswith E3-CaM in ubiquitinating calmodulin;interacts with many SCF ubiquitin proteinligases; component of the cellular stressresponse 0.008070.09126&radic&radic S000000421ATG12YBR217WverifiedUbiquitin-like modifier, conjugated via an isopeptide bond to alysine residue of Atg5p by the E1 enzyme,Atg7p, and the E2 enzyme, Atg10p, a step thatis essential for autophagy 0.008050.09126&radic&radic&radic S000002546RUB1YDR139CverifiedUbiquitin-like protein with similarity to mammalian NEDD8;conjugation (neddylation) substrates includethe cullins Cdc53p, Rtt101p, and Cul3p;activated by Ula1p and Uba3p (E1 enzyme pair);conjugation mediated by Ubc12p (E2 enzyme) 0.008070.09126&radic&radic&radic S000004523SML1YML058WverifiedRibonucleotide reductase inhibitor involved in regulating dNTPproduction; regulated by Mec1p and Rad53pduring DNA damage and S phase 0.008050.09126&radic&radic S000000786PRB1YEL060CverifiedVacuolar proteinase B (yscB), a serine protease of thesubtilisin family; involved in proteindegradation in the vacuole and required forfull protein degradation during sporulation 0.008050.09126&radic&radic&radic&radic&radic S000004364SUR4YLR372WverifiedElongase, involved in fatty acid and sphingolipid biosynthesis;synthesizes very long chain 20-26-carbon fattyacids from C18-CoA primers; involved inregulation of sphingolipid biosynthesis 0.008030.09103 S000005079FPR1YNL135CverifiedPeptidyl-prolyl cis-trans isomerase (PPIase), binds to thedrugs FK506 and rapamycin; also binds to thenonhistone chromatin binding protein Hmo1p andmay regulate its assembly or function 0.008010.09048&radic&radic S000004550TUB1YML085CverifiedAlpha-tubulin; associates with beta-tubulin (Tub2p) to formtubulin dimer, which polymerizes to formmicrotubules 0.007980.08951&radic&radic&radic S000006368MMS1YPR164WverifiedProtein likely involved in protection againstreplication-dependent DNA damage; mutants aresensitive to methyl methanesulfonate (MMS);implicated in regulation of Ty1 transposition 0.007980.08951&radic&radic S000004065RPL10YLR075WverifiedProtein component of the large (60S) ribosomal subunit,responsible for joining the 40S and 60Ssubunits; regulates translation initiation; hassimilarity to rat L10 ribosomal protein and tomembers of the QM gene family 0.007980.08951&radic&radic&radic&radic&radic S000002793EFT2YDR385WverifiedElongation factor 2 (EF-2), also encoded by EFT1; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 0.007950.08951&radic&radic&radic&radic S000000520BUD3YCL014WverifiedProtein involved in bud-site selection and required for axialbudding pattern; localizes with septins to budneck in mitosis and may constitute an axiallandmark for next round of budding 0.007930.08951&radic&radic S000000036CDC19YAL038WverifiedPyruvate kinase, functions as a homotetramer in glycolysis toconvert phosphoenolpyruvate to pyruvate, theinput for aerobic (TCA cycle) or anaerobic(glucose fermentation) respiration 0.00790.089&radic&radic&radic&radic S000005133SRP1YNL189WverifiedKaryopherin alpha homolog, forms a dimer with karyopherin betaKap95p to mediate import of nuclear proteins,binds the nuclear localization signal of thesubstrate during import; may also play a rolein regulation of protein degradation 0.007910.089&radic&radic&radic&radic&radic S000006147NEW1YPL226WverifiedATP binding cassette family member; Asn/Gln-rich rich regionsupports [NU+] prion formation, susceptibilityto [PSI+] prion induction and aggregation of afragment of the human Machado-Joseph Diseaseprotein 0.007920.089&radic&radic&radic&radic S000006027SSE1YPL106CverifiedATPase that is a component of the heat shock protein Hsp90chaperone complex; binds unfolded proteins;member of the heat shock protein 70 (HSP70)family; localized to the cytoplasm 0.007920.089&radic&radic&radic&radic&radic S000004282GCD7YLR291CverifiedBeta subunit of the translation initiation factor eIF2B, theguanine-nucleotide exchange factor for eIF2;activity subsequently regulated byphosphorylated eIF2; first identified as anegative regulator of GCN4 expression 0.007870.08818&radic&radic&radic&radic&radic&radic S000002719TFB1YDR311WverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, required for nucleotide excisionrepair, target for transcriptional activators 0.007840.08818&radic S000000221KAP104YBR017CverifiedTransportin, cytosolic karyopherin beta 2 involved in deliveryof heterogeneous nuclear ribonucleoproteins tothe nucleoplasm, binds rg-nuclear localizationsignals on Nab2p and Hrp1p, plays a role incell-cycle progression 0.007840.08818&radic S000003319PDC6YGR087CverifiedMinor isoform of pyruvate decarboxylase, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde, regulation is glucose- andethanol-dependent, involved in amino acidcatabolism 0.007790.08742&radic&radic&radic&radic&radic S000000015PSK1YAL017WverifiedOne of two (see also PSK2) PAS domain containing S/T proteinkinases; coordinately regulates proteinsynthesis and carbohydrate metabolism andstorage in response to a unknown metabolitethat reflects nutritional status 0.00780.08742&radic&radic&radic S000000897RAD51YER095WverifiedStrand exchange protein, forms a helical filament with DNA thatsearches for homology; involved in therecombinational repair of double-strand breaksin DNA during vegetative growth and meiosis;homolog of Dmc1p and bacterial RecA protein 0.007820.08742&radic S000003461SMI1YGR229CverifiedProtein involved in the regulation of cell wall synthesis;proposed to be involved in coordinating cellcycle progression with cell wall integrity 0.007780.08742&radic&radic&radic&radic&radic S000003074MLC1YGL106WverifiedEssential light chain for myosin Myo2p; may stabilize Myo2p bybinding to the neck region; may interact withMyo1p, Iqg1p, and Myo2p to coordinate formationand contraction of the actomyosin ring withtargeted membrane deposition 0.007750.0869 S000006339CTF4YPR135WverifiedChromatin-associated protein, required for sister chromatidcohesion; interacts with DNA polymerase alpha(Pol1p) and may link DNA synthesis to sisterchromatid cohesion 0.007760.0869&radic&radic S000005662IDH2YOR136WverifiedSubunit of mitochondrial NAD(+)-dependent isocitratedehydrogenase, which catalyzes the oxidation ofisocitrate to alpha-ketoglutarate in the TCAcycle 0.007710.08639&radic&radic S000004721FMP24YMR115WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.007710.08639 S000004370SEC61YLR378CverifiedEssential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p);forms a channel for SRP-dependent proteinimport and retrograde transport of misfoldedproteins out of the ER; with Sec63 complexallows SRP-independent protein import into ER 0.007730.08639&radic&radic&radic&radic S000000284SEC18YBR080CverifiedATPase required for the release of Sec17p during the 'priming'step in homotypic vacuole fusion and for ER toGolgi transport; homolog of the mammalian NSF 0.007670.08554&radic S000003606YJL070CUncharacterizedPutative protein of unknown function with similarity to AMP deaminases; YJL070C is a non-essential gene 0.00770.08554&radic&radic S000000516SGF29YCL010CverifiedSaGa associated Factor 29kDa; Probable 29kKDa Subunit of SAGAhistone acetyltransferase complex 0.007650.08554&radic S000001177YCK1YHR135CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck2p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck2p 0.00770.08554&radic&radic&radic&radic S000002284CDC48YDL126CverifiedATPase in ER, nuclear membrane and cytosol with homology tomammalian p97; in a complex with Npl4p andUfd1p participates in retrotranslocation ofubiquitinated proteins from the ER into thecytosol for degradation by the proteasome 0.007690.08554&radic&radic S000005477RRI2YOL117WverifiedSubunit of the COP9 signalosome (CSN) complex that cleaves theubiquitin-like protein Nedd8 from SCF ubiquitinligases; plays a role in the mating pheromoneresponse 0.007650.08554&radic S000005339BIO5YNR056CverifiedPutative transmembrane protein involved in the biotinbiosynthesis pathway; responsible for uptake of7-keto 8-aminopelargonic acid; BIO5 is in acluster of 3 genes (BIO3, BIO4, and BIO5) thatmediate biotin synthesis 0.007690.08554 S000001635GPM1YKL152CverifiedTetrameric phosphoglycerate mutase, mediates the conversion of3-phosphoglycerate to 2-phosphoglycerate duringglycolysis and the reverse reaction duringgluconeogenesis 0.007690.08554&radic&radic&radic&radic&radic&radic S000001458MUC1YIR019CverifiedGPI-anchored cell surface glycoprotein required for diploidpseudohyphal formation and haploid invasivegrowth, transcriptionally regulated by the MAPKpathway (via Ste12p and Tec1p) and the cAMPpathway (via Flo8p) 0.007640.08554&radic&radic&radic&radic&radic S000006363KRE6YPR159WverifiedProtein required for beta-1,6 glucan biosynthesis; putativebeta-glucan synthase; appears functionallyredundant with Skn1p 0.007630.08487&radic S000004897YKU70YMR284WverifiedSubunit of the telomeric Ku complex (Yku70p-Yku80p), involvedin telomere length maintenance, structure andtelomere position effect; relocates to sites ofdouble-strand cleavage to promote nonhomologousend joining during DSB repair 0.007630.08487&radic&radic&radic&radic&radic S000003827TOR1YJR066WverifiedPIK-related protein kinase and rapamycin target; subunit ofTORC1, a complex that controls growth inresponse to nutrients by regulatingtranslation, transcription, ribosomebiogenesis, nutrient transport and autophagy;involved in meiosis 0.007620.08487&radic&radic S000005061MLS1YNL117WverifiedMalate synthase, enzyme of the glyoxylate cycle, involved inutilization of non-fermentable carbon sources;expression is subject to carbon cataboliterepression; localizes in peroxisomes duringgrowth in oleic acid medium 0.007620.08487&radic S000002536SAC6YDR129CverifiedFimbrin, actin-bundling protein; cooperates with Scp1p(calponin/transgelin) in the organization andmaintenance of the actin cytoskeleton 0.00760.08487&radic&radic&radic&radic&radic S000003952YLL029WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL029W is not an essential gene but mutant is defective in spore formation 0.007570.08446&radic&radic&radic&radic S000002987CKB1YGL019WverifiedBeta regulatory subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA1, CKA2 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 0.007550.08406&radic&radic&radic&radic S000001132YNG2YHR090CverifiedSubunit of the NuA4 histone acetyltransferase complex thatacetylates histone H4 and H2A; has similarityto the human tumor suppressor ING1 0.007540.08406 S000001686TOR2YKL203CverifiedPIK-related protein kinase and rapamycin target; subunit ofTORC1, a complex that regulates growth inresponse to nutrients and TORC2, a complex thatregulates cell-cycle dependent polarization ofthe actin cytoskeleton; involved in meiosis 0.007560.08406&radic&radic&radic&radic S000004652ERB1YMR049CverifiedProtein required for maturation of the 25S and 5.8S ribosomalRNAs; constituent of 66S pre-ribosomalparticles; homologous to mammalian Bop1 0.007540.08406 S000003055MMS2YGL087CverifiedProtein involved in error-free postreplication DNA repair;forms a heteromeric complex with Ubc13p thathas a ubiquitin-conjugating activity;cooperates with chromatin-associated RINGfinger proteins, Rad18p and Rad5p 0.007560.08406&radic&radic&radic&radic&radic&radic S000000292POL30YBR088CverifiedProliferating cell nuclear antigen (PCNA), functions as thesliding clamp for DNA polymerase delta; mayfunction as a docking site for other proteinsrequired for mitotic and meiotic chromosomalDNA replication and for DNA repair 0.007540.08406&radic&radic S000003588TDH1YJL052WverifiedGlyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involvedin glycolysis and gluconeogenesis; tetramerthat catalyzes the reaction ofglyceraldehyde-3-phosphate to 1,3bis-phosphoglycerate; detected in the cytoplasmand cell-wall 0.007520.08336&radic&radic S000001212DBP8YHR169WverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in biogenesis of the 40S ribosomalsubunit 0.007540.08336 S000003778ESS1YJR017CverifiedPeptidylprolyl-cis/trans-isomerase (PPIase) specific forphosphorylated serine and threonine residuesN-terminal to proline; regulatesphosphorylation of the RNA polymerase II largesubunit (Rpo21p) C-terminal domain 0.007520.08336&radic&radic&radic S000003484GCN5YGR252WverifiedHistone acetyltransferase, acetylates N-terminal lysines onhistones H2B and H3; catalytic subunit of theADA and SAGA histone acetyltransferasecomplexes; founding member of the Gcn5p-relatedN-acetyltransferase superfamily 0.007530.08336 S000004312MMS22YLR320WverifiedProtein involved in resistance to ionizing radiation; acts withMms1p in a repair pathway that may be involvedin resolving replication intermediates orpreventing the damage caused by blockedreplication forks 0.00750.08336&radic S000004166RFX1YLR176CverifiedProtein involved in DNA damage and replication checkpointpathway; recruits repressors Tup1p and Cyc8p topromoters of DNA damage-inducible genes;similar to a family of mammalian DNA bindingRFX1-4 proteins 0.007530.08336&radic&radic&radic S000006273SPE3YPR069CverifiedSpermidine synthase, involved in biosynthesis of spermidine andalso in biosynthesis of pantothenic acid;spermidine is required for growth of wild-typecells 0.007520.08336&radic S000000001TFC3YAL001CverifiedLargest of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauB domain of TFIIIC that binds DNA at theBoxB promoter sites of tRNA and similar genes;cooperates with Tfc6p in DNA binding 0.007520.08336&radic&radic&radic&radic&radic S000001649TPK3YKL166CverifiedSubunit of cAMP-dependent protein kinase, which contains Tpk1p,Tpk2p, Tpk3p, and regulatory subunit Bcy1p;promotes growth in response to nutrients;mediates induction of apoptosis due toactin-mediated hyperactivation of Ras signaling 0.007460.08285&radic&radic S000006260TFB4YPR056WverifiedSubunit of TFIIH complex, involved in transcription initiation,similar to 34 kDa subunit of human TFIIH;interacts with Ssl1p 0.007460.08285 S000005173URE2YNL229CverifiedNitrogen catabolite repression regulator that acts byinhibition of GLN3 transcription in goodnitrogen source; altered form of Ure2p creates[URE3] prion 0.007480.08285&radic&radic&radic&radic S000002806UTP5YDR398WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.007480.08285&radic&radic&radic&radic S000001283RPB3YIL021WverifiedRNA polymerase II third largest subunit B44, part of centralcore; similar to prokaryotic alpha subunit 0.007440.08273&radic&radic S000002178RPN4YDL020CverifiedTranscription factor that stimulates expression of proteasomegenes; Rpn4p levels are in turn regulated bythe 26S proteasome in a negative feedbackcontrol mechanism; RPN4 is transcriptionallyregulated by various stress responses 0.007450.08273&radic S000000838ARB1YER036CverifiedATPase of the ATP-binding cassette (ABC) family involved in 40Sand 60S ribosome biogenesis, has similarity toGcn20p; shuttles from nucleus to cytoplasm,physically interacts with Tif6p, Lsg1p 0.007450.08273&radic&radic&radic S000000979BMH1YER177Wverified14-3-3 protein, major isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.007430.08251&radic S000001578YJU2YKL095WverifiedEssential nuclear protein; putative spliceosomal componentinvolved in mRNA splicing, based oncomputational analysis of large-scaleprotein-protein interaction data 0.007440.08251&radic&radic&radic S000002314CLB3YDL155WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the G2/M transition; may beinvolved in DNA replication and spindleassembly; accumulates during S phase and G2,then targeted for ubiquitin-mediateddegradation 0.007440.08251&radic&radic S000000362AMN1YBR158WverifiedProtein required for daughter cell separation, multiple mitoticcheckpoints, and chromosome stability; contains12 degenerate leucine-rich repeat motifs;expression is induced by the Mitotic ExitNetwork (MEN) 0.007380.08141&radic S000006238ARP7YPR034WverifiedActin-related protein involved in transcriptional regulation;subunit of the chromatin remodeling Snf/Swicomplex 0.007420.08141 S000002448RSM10YDR041WverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S10 ribosomal protein;essential for viability, unlike most othermitoribosomal proteins 0.007370.08141&radic S000005544ROD1YOR018WverifiedMembrane protein; overexpression confers resistance to the GSTsubstrate o-dinitrobenzene as well as to zincand calcium; contains 2 PY motifs, which arerequired for Rod1p interaction with Rsp5p, ahect-type ubiquitin ligase 0.007370.08141&radic&radic&radic&radic&radic S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 0.007360.08141&radic&radic&radic S000005612TCB1YOR086CverifiedLipid-binding protein containing three calcium and lipidbinding domains; non-tagged protein localizesto mitochondria and GFP-fusion proteinlocalizes to the cell periphery; C-termini ofTcb1p, Tcb2p and Tcb3p interact 0.007420.08141&radic&radic&radic&radic&radic S000002255RPN6YDL097CverifiedEssential, non-ATPase regulatory subunit of the 26S proteasomelid required for the assembly and activity ofthe 26S proteasome; the human homolog (S9protein) partially rescues Rpn6p depletion 0.007370.08141&radic&radic S000001596RAD27YKL113Cverified5' to 3' exonuclease, 5' flap endonuclease, required forOkazaki fragment processing and maturation aswell as for long-patch base-excision repair;member of the S. pombe RAD2/FEN1 family 0.007370.08141&radic&radic&radic&radic&radic S000002411RAD57YDR004WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad55p 0.007420.08141&radic S000004295ACO1YLR304CverifiedAconitase, required for the tricarboxylic acid (TCA) cycle andalso independently required for mitochondrialgenome maintenance; component of themitochondrial nucleoid; mutation leads toglutamate auxotrophy 0.007370.08141&radic&radic S000000022LTE1YAL024CverifiedPutative GDP/GTP exchange factor required for mitotic exit atlow temperatures; acts as a guanine nucleotideexchange factor (GEF) for Tem1p, which is a keyregulator of mitotic exit; physicallyassociates with Ras2p-GTP 0.007430.08141&radic&radic S000000867ICL1YER065CverifiedIsocitrate lyase, catalyzes the formation of succinate andglyoxylate from isocitrate, a key reaction ofthe glyoxylate cycle; expression of ICL1 isinduced by growth on ethanol and repressed bygrowth on glucose 0.007340.08113&radic&radic&radic&radic&radic S000005000POR1YNL055CverifiedMitochondrial porin (voltage-dependent anion channel), outermembrane protein required for the maintenanceof mitochondrial osmotic stability andmitochondrial membrane permeability 0.007330.081&radic S000004197HRD3YLR207WverifiedResident protein of the ER membrane that plays a central rolein ER-associated protein degradation (ERAD),forms HRD complex with Hrd1p and ERADdeterminants that engages in lumen to cytosolcommunication and coordination of ERAD events &radic0.007320.081&radic&radic S000002740IRC3YDR332WuncharacterizedHypothetical protein; null mutant displays increased levels ofspontaneous Rad52 foci 0.007190.07819&radic&radic&radic S000004124PDC5YLR134WverifiedMinor isoform of pyruvate decarboxylase, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde, regulation is glucose- andethanol-dependent, repressed by thiamine,involved in amino acid catabolism 0.007180.07819&radic S000005677RPB2YOR151CverifiedRNA polymerase II second largest subunit B150, part of centralcore; similar to bacterial beta subunit 0.00720.07819&radic&radic&radic&radic&radic&radic S000003080TAF6YGL112CverifiedSubunit (60 kDa) of TFIID and SAGA complexes, involved intranscription initiation of RNA polymerase IIand in chromatin modification, similar tohistone H4 0.007190.07819 S000000132YBL036CVerifiedSingle-domain racemase, possibly non-specific due to the lack of the second domain, which presumably determines specificity 0.007250.07819&radic&radic S000001153UBA4YHR111WverifiedProtein that activates Urm1p before its conjugation to proteins(urmylation); one target is the thioredoxinperoxidase Ahp1p, suggesting a role ofurmylation in the oxidative stress response 0.007160.07819&radic&radic&radic&radic&radic&radic S000001106SSZ1YHR064CverifiedHsp70 protein that interacts with Zuo1p (a DnaJ homolog) toform a ribosome-associated complex that bindsthe ribosome via the Zuo1p subunit; alsoinvolved in pleiotropic drug resistance viasequential activation of PDR1 and PDR5; bindsATP 0.007260.07819&radic&radic S000005551HST3YOR025WverifiedMember of the Sir2 family of NAD(+)-dependent proteindeacetylases; involved along with Hst4p intelomeric silencing, cell cycle progression,radiation resistance, genomic stability andshort-chain fatty acid metabolism 0.007290.07819&radic&radic&radic&radic S000001001YAP3YHL009CverifiedBasic leucine zipper (bZIP) transcription factor 0.007250.07819&radic&radic&radic&radic&radic S000003769TDH2YJR009CverifiedGlyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involvedin glycolysis and gluconeogenesis; tetramerthat catalyzes the reaction ofglyceraldehyde-3-phosphate to 1,3bis-phosphoglycerate; detected in the cytoplasmand cell-wall 0.007220.07819&radic&radic S000005121MRPL22YNL177CverifiedMitochondrial ribosomal protein of the large subunit 0.00730.07819&radic&radic&radic&radic S000006098CUP9YPL177CverifiedHomeodomain-containing transcriptional repressor of PTR2, whichencodes a major peptide transporter; importedpeptides activate ubiquitin-dependentproteolysis, resulting in degradation of Cup9pand de-repression of PTR2 transcription 0.007220.07819&radic&radic&radic S000005768SSP2YOR242CverifiedSporulation specific protein that localizes to the spore wall;required for sporulation at a point aftermeiosis II and during spore wall formation;SSP2 expression is induced midway in meiosis 0.007320.07819&radic&radic&radic&radic S000003494BUD32YGR262CverifiedProtein kinase proposed to be involved in bud-site selection,telomere uncapping and elongation, andtranscription; component of the EKC/KEOPSprotein complex with Kae1p, Cgi121p, Pcc1p, andGon7p 0.00730.07819&radic&radic&radic&radic S000001521RGT1YKL038WverifiedGlucose-responsive transcription factor that regulatesexpression of several glucose transporter (HXT)genes in response to glucose; binds topromoters and acts both as a transcriptionalactivator and repressor 0.007260.07819&radic&radic&radic&radic&radic&radic&radic&radic S000002578HSP42YDR171WverifiedSmall cytosolic stress-induced chaperone that formsbarrel-shaped oligomers and suppresses theaggregation of non-native proteins; oligomerdissociation is not required for function;involved in cytoskeleton reorganization afterheat shock 0.00730.07819&radic&radic&radic&radic&radic S000004219CDC42YLR229CverifiedSmall rho-like GTPase, essential for establishment andmaintenance of cell polarity; mutants havedefects in the organization of actin andseptins 0.007190.07819 S000001445PAN1YIR006CverifiedPart of actin cytoskeleton-regulatory complexPan1p-Sla1p-End3p, associates with actinpatches on the cell cortex; promotesprotein-protein interactions essential forendocytosis; previously thought to be a subunitof poly(A) ribonuclease 0.00720.07819&radic S000003829RFC2YJR068WverifiedSubunit of heteropentameric Replication factor C (RF-C), whichis a DNA binding protein and ATPase that actsas a clamp loader of the proliferating cellnuclear antigen (PCNA) processivity factor forDNA polymerases delta and epsilon 0.007190.07819&radic S000004185NMT1YLR195CverifiedN-myristoyl transferase, catalyzes the cotranslational,covalent attachment of myristic acid to theN-terminal glycine residue of several proteinsinvolved in cellular growth and signaltransduction 0.007260.07819&radic&radic&radic&radic&radic S000001234CTF8YHR191CverifiedSubunit of a complex with Ctf18p that shares some subunits withReplication Factor C and is required for sisterchromatid cohesion 0.007150.07777&radic S000003963VPS13YLL040CverifiedProtein of unknown function; heterooligomeric or homooligomericcomplex; peripherally associated withmembranes; homologous to human COH1; involvedin sporulation, vacuolar protein sorting andprotein-Golgi retention 0.007130.07777&radic&radic&radic&radic&radic S000002553SWI5YDR146CverifiedTranscription factor that activates transcription of genesexpressed at the M/G1 phase boundary and in G1phase; localization to the nucleus occursduring G1 and appears to be regulated byphosphorylation by Cdc28p kinase 0.007120.07774&radic&radic&radic S000003457AMA1YGR225WverifiedActivator of meiotic anaphase promoting complex (APC/C); Cdc20pfamily member; required for initiation of sporewall assembly; required for Clb1p degradationduring meiosis 0.007120.07774&radic&radic&radic S000003049YGL081WUncharacterizedHypothetical protein 0.007120.07766 S000001022ECM29YHL030WverifiedMajor component of the proteasome; tethers the proteasome coreparticle to the regulatory particle, andenhances the stability of the proteasome 0.007120.07766&radic&radic&radic&radic&radic S000002259DUN1YDL101CverifiedCell-cycle checkpoint serine-threonine kinase required for DNAdamage-induced transcription of certain targetgenes, phosphorylation of Rad55p and Sml1p, andtransient G2/M arrest after DNA damage; alsoregulates postreplicative DNA repair 0.007110.07754 S000004306CDC3YLR314CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM 0.007110.07754&radic&radic&radic S000006180APM1YPL259CverifiedMu1-like medium subunit of the clathrin-associated proteincomplex (AP-1); binds clathrin; involved inclathrin-dependent Golgi protein sorting 0.007090.07654&radic&radic&radic&radic S000000023MAK16YAL025CverifiedEssential nuclear protein, constituent of 66S pre-ribosomalparticles; required for maturation of 25S and5.8S rRNAs; required for maintenance of M1satellite double-stranded RNA of the L-A virus 0.007070.07654&radic&radic S000000630FEN1YCR034WverifiedFatty acid elongase, involved in sphingolipid biosynthesis;acts on fatty acids of up to 24 carbons inlength; mutations have regulatory effects on1,3-beta-glucan synthase, vacuolar ATPase, andthe secretory pathway 0.007090.07654&radic S000000824SRB4YER022WverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation 0.007070.07654&radic&radic&radic&radic S000001131GAR1YHR089CverifiedProtein component of the H/ACA snoRNP pseudouridylase complex,involved in the modification and cleavage ofthe 18S pre-rRNA 0.007070.07654&radic S000000287TEC1YBR083WverifiedTranscription factor required for full Ty1 epxression,Ty1-mediated gene activation, and haploidinvasive and diploid pseudohyphal growth;TEA/ATTS DNA-binding domain family member 0.007080.07654&radic&radic S000003324DBF2YGR092WverifiedSer/Thr kinase involved in transcription and stress response;functions as part of a network of genes in exitfrom mitosis; localization is cell cycleregulated; activated by Cdc15p during the exitfrom mitosis 0.007060.07654&radic S000002743MSN5YDR335WverifiedKaryopherin involved in nuclear import and export; shown to beresponsible for nuclear import of replicationprotein A and for export of several proteinsincluding Swi6p, Far1p, and Pho4p; cargodissociation involves binding to RanGTP 0.007020.07585&radic&radic&radic S000001628RPT1YKL145WverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for optimalCDC20 transcription; interacts with Rpn12p andthe E3 ubiquitin-protein ligase Ubr1p 0.007040.07585&radic&radic S000005122RPS3YNL178WverifiedProtein component of the small (40S) ribosomal subunit, hasapurinic/apyrimidinic (AP) endonucleaseactivity; essential for viability; hassimilarity to E. coli S3 and rat S3 ribosomalproteins 0.007050.07585&radic&radic&radic&radic S000003517ZUO1YGR285CverifiedCytosolic ribosome-associated chaperone that acts, togetherwith Ssz1p and the Ssb proteins, as a chaperonefor nascent polypeptide chains; contains a DnaJdomain and functions as a J-protein partner forSsb1p and Ssb2p 0.007040.07585&radic&radic&radic&radic&radic&radic S000000913SWI4YER111CverifiedDNA binding component of the SBF complex (Swi4p-Swi6p), atranscriptional activator that in concert withMBF (Mbp1-Swi6p) regulates late G1-specifictranscription of targets including cyclins andgenes required for DNA synthesis and repair 0.006990.07484&radic S000002931SPS1YDR523CverifiedPutative protein serine/threonine kinase expressed at the endof meiosis and localized to the prosporemembrane, required for correct localization ofenzymes involved in spore wall synthesis 0.0070.07484&radic S000005859VMA4YOR332WverifiedSubunit E of the eight-subunit V1 peripheral membrane domain ofthe vacuolar H+-ATPase (V-ATPase), anelectrogenic proton pump found throughout theendomembrane system; required for the V1 domainto assemble onto the vacuolar membrane 0.006970.07484&radic&radic&radic&radic S000005643RPT5YOR117WverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; recruited to theGAL1-10 promoter region upon induction oftranscription 0.006960.07484&radic&radic S000001293ULP2YIL031WverifiedPeptidase that deconjugates Smt3/SUMO-1 peptides from proteins,plays a role in chromosome cohesion atcentromeric regions and recovery fromcheckpoint arrest induced by DNA damage or DNAreplication defects; potential Cdc28p substrate 0.0070.07484&radic S000004593TUB3YML124CverifiedAlpha-tubulin; associates with beta-tubulin (Tub2p) to formtubulin dimer, which polymerizes to formmicrotubules; expressed at lower level thanTub1p 0.006990.07484&radic&radic S000005759KIN4YOR233WverifiedKinase that acts by inhibiting the mitotic exit network (MEN)when the spindle position checkpoint isactivated; localized asymmetrically to mothercell cortex, spindle pole body and bud neck 0.006980.07484&radic&radic&radic&radic&radic S000000399MSI1YBR195CverifiedSubunit of chromatin assembly factor I (CAF-I), regulates theRAS/cAMP pathway via sequestration of Npr1pkinase; localizes to the nucleus and cytoplasm;homologous to human retinoblastoma bindingproteins RbAp48 and RbAp46 0.006980.07484&radic&radic&radic&radic&radic S000001779DRE2YKR071CverifiedProtein of unknown function; mutation displays synthetic lethalinteraction with the pol3-13 allele of CDC2 0.0070.07484&radic&radic&radic&radic&radic S000006373JIP5YPR169WuncharacterizedNucleolar protein of unknown function, exhibits a physicalinteraction with Bre1p 0.006960.07452&radic&radic&radic S000000128HEK2YBL032WverifiedRNA binding protein with similarity to hnRNP-K that localizesto the cytoplasm and to subtelomeric DNA;required for the proper localization of ASH1mRNA; involved in the regulation of telomereposition effect and telomere length 0.006960.07452&radic&radic&radic&radic S000004140STM1YLR150WverifiedProtein that binds G4 quadruplex and purine motif triplexnucleic acid; acts with Cdc13p to maintaintelomere structure; interacts with ribosomesand subtelomeric Y' DNA; multicopy suppressorof tom1 and pop2 mutations 0.006940.07407&radic&radic&radic&radic&radic&radic&radic S000004470SPT5YML010WverifiedProtein that forms a complex with Spt4p and mediates bothactivation and inhibition of transcriptionelongation; Spt4p-Spt5p complex also plays arole in pre-mRNA processing 0.006930.07407&radic&radic&radic&radic&radic&radic&radic S000006062YPL141CUncharacterizedHypothetical protein 0.006930.07407&radic&radic&radic&radic&radic S000001324ARC15YIL062CverifiedSubunit of the ARP2/3 complex, which is required for themotility and integrity of cortical actinpatches 0.006920.07394&radic S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.006890.07323&radic&radic&radic&radic&radic&radic&radic S000000025YAL027WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YAL027W is a non-essential gene 0.006880.07323&radic&radic S000005947SKS1YPL026CverifiedPutative serine/threonine protein kinase; involved in theadaptation to low concentrations of glucoseindependent of the SNF3 regulated pathway 0.00690.07323&radic&radic&radic&radic&radic&radic S000001472MGA2YIR033WverifiedER membrane protein involved in regulation of OLE1transcription, acts with homolog Spt23p;inactive ER form dimerizes and one subunit isthen activated byubiquitin/proteasome-dependent processingfollowed by nuclear targeting 0.006850.07228&radic S000003218ZIP2YGL249WverifiedMeiosis-specific protein involved in normal synaptonemalcomplex formation and pairing betweenhomologous chromosomes during meiosis 0.006840.07228&radic&radic S000005724BFR1YOR198CverifiedComponent of mRNP complexes associated with polyribosomes;implicated in secretion and nuclearsegregation; multicopy suppressor of BFA(Brefeldin A) sensitivity 0.006840.0721&radic&radic&radic&radic S000005787RPN8YOR261CverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome;has similarity to the human p40 proteasomalsubunit and to another S. cerevisiae regulatorysubunit, Rpn11p 0.006820.07178&radic&radic&radic&radic&radic&radic S000004248GSY2YLR258WverifiedGlycogen synthase, similar to Gsy1p; expression induced byglucose limitation, nitrogen starvation, heatshock, and stationary phase; activity regulatedby cAMP-dependent, Snf1p and Pho85p kinases aswell as by the Gac1p-Glc7p phosphatase 0.006810.07178&radic&radic&radic&radic S000002742SWR1YDR334WverifiedSwi2/Snf2-related ATPase that is the structural component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.006790.07095&radic&radic&radic&radic&radic&radic&radic S000000374NPL4YBR170CverifiedEndoplasmic reticulum and nuclear membrane protein, forms acomplex with Cdc48p and Ufd1p that recognizesubiquitinated proteins in the endoplasmicreticulum and delivers them to the proteasomefor degradation 0.006780.07095&radic S000000291RFC5YBR087WverifiedSubunit of heteropentameric Replication factor C (RF-C), whichis a DNA binding protein and ATPase that actsas a clamp loader of the proliferating cellnuclear antigen (PCNA) processivity factor forDNA polymerases delta and epsilon 0.00680.07095 S000000945DDI1YER143WverifiedDNA damage-inducible v-SNARE binding protein, contains aubiquitin-associated (UBA) domain, may act as anegative regulator of constitutive exocytosis,may play a role in S-phase checkpoint control 0.006790.07095&radic S000000019CCR4YAL021CverifiedComponent of the CCR4-NOT transcriptional complex, which isinvolved in regulation of gene expression;component of the major cytoplasmic deadenylase,which is involved in mRNA poly(A) tailshortening 0.00680.07095&radic&radic&radic&radic S000006130IPL1YPL209CverifiedAurora kinase involved in regulating kinetochore-microtubuleattachments; helps maintain condensedchromosomes during anaphase and earlytelophase; associates with Sli15p, whichpromotes Ipl1p association with mitotic spindle 0.006760.07054&radic&radic&radic&radic&radic&radic&radic&radic S000000529KCC4YCL024WverifiedProtein kinase of the bud neck involved in the septincheckpoint, associates with septin proteins,negatively regulates Swe1p by phosphorylation,shows structural homology to bud neck kinasesGin4p and Hsl1p 0.006760.07054&radic&radic&radic&radic S000002685MTH1YDR277CverifiedNegative regulator of the glucose-sensing signal transductionpathway, required for repression oftranscription by Rgt1p; interacts with Rgt1pand the Snf3p and Rgt2p glucose sensors;phosphorylated by Yck1p, triggering Mth1pdegradation 0.006770.07054&radic&radic&radic S000002198NAT1YDL040CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.006780.07054&radic&radic S000004073EMP70YLR083CverifiedProtein whose 24kDa cleavage product is found inendosome-enriched membrane fractions, predictedto be a transmembrane protein 0.006760.07054&radic&radic&radic&radic S000002260CDC2YDL102WverifiedCatalytic subunit of DNA polymerase delta; required forchromosomal DNA replication during mitosis andmeiosis, intragenic recombination, repair ofdouble strand DNA breaks, and DNA replicationduring nucleotide excision repair (NER) 0.006780.07054 S000005564HIR2YOR038CverifiedNon-essential transcriptional corepressor involved in the cellcycle-regulated transcription of histone H2A,H2B, H3, and H4 genes; recruits Swi-Snfcomplexes to histone gene promoters; promotesheterochromatic gene silencing with Asf1p 0.006750.07039&radic&radic&radic&radic&radic&radic S000003660LSM1YJL124CverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)involved in degradation of cytoplasmic mRNAs 0.006750.07039&radic&radic S000003112ROG1YGL144CverifiedProtein with putative serine active lipase domain 0.006750.07026 S000005098YCK2YNL154CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck1p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck1p 0.006750.07026&radic&radic&radic&radic S000000072CDC15YAR019CverifiedProtein kinase of the Mitotic Exit Network that is localized tothe spindle pole bodies at late anaphase;promotes mitotic exit by directly switching onthe kinase activity of Dbf2p 0.006740.07001 S000006065YPL144WUncharacterizedHypothetical protein 0.006740.07001&radic&radic&radic&radic&radic S000000564KRR1YCL059CverifiedEssential nucleolar protein required for the synthesis of 18SrRNA and for the assembly of 40S ribosomalsubunit 0.006740.07001&radic&radic&radic&radic&radic S000004847RNH1YMR234WverifiedRibonuclease H1, removes RNA primers during Okazaki fragmentsynthesis; degrades RNA attached to the 5'-endof a DNA strand 0.006720.06962&radic&radic&radic&radic S000000263AKL1YBR059CverifiedSer-Thr protein kinase, member (with Ark1p and Prk1p) of theArk kinase family; involved in endocytosis andactin cytoskeleton organization 0.006720.06962&radic&radic S000002189DBP10YDL031WverifiedPutative ATP-dependent RNA helicase of the DEAD-box proteinfamily, constituent of 66S pre-ribosomalparticles; essential protein involved inribosome biogenesis 0.006720.06962 S000005080EAF7YNL136WverifiedSubunit of the NuA4 histone acetyltransferase complex, whichacetylates the N-terminal tails of histones H4and H2A 0.006710.06962 S000004871YMR258CUncharacterizedProtein of unknown function with similarity to F-box proteins; physically interacts with Skp1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR258C is not an essential gene 0.006730.06962&radic&radic S000001055ARD1YHR013CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.006720.06962&radic&radic&radic&radic&radic S000001665FAS1YKL182WverifiedBeta subunit of fatty acid synthetase, which catalyzes thesynthesis of long-chain saturated fatty acids;contains acetyltransacylase, dehydratase, enoylreductase, malonyl transacylase, and palmitoyltransacylase activities 0.00670.06932&radic&radic&radic&radic&radic S000006288YPR084WUncharacterizedHypothetical protein 0.006630.06745&radic S000002622AHA1YDR214WverifiedCo-chaperone that binds to Hsp82p and activates its ATPaseactivity; similar to Hch1p; expression isregulated by stresses such as heat shock 0.006640.06745&radic&radic&radic S000002910SAM2YDR502CverifiedS-adenosylmethionine synthetase, catalyzes transfer of theadenosyl group of ATP to the sulfur atom ofmethionine; one of two differentially regulatedisozymes (Sam1p and Sam2p) 0.006670.06745&radic&radic S000005067NMA111YNL123WverifiedProtein of unknown function which may contribute to lipidhomeostasis and/or apoptosis; sequencesimilarity to the mammalian Omi/HtrA2 family ofserine proteases 0.006660.06745 S000005251MCK1YNL307CverifiedProtein serine/threonine/tyrosine (dual-specificity) kinaseinvolved in control of chromosome segregationand in regulating entry into meiosis; relatedto mammalian glycogen synthase kinases of theGSK-3 family 0.006660.06745&radic&radic&radic&radic&radic S000003837CDC11YJR076CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM 0.006620.06745 S000001504MAK11YKL021CverifiedProtein essential for cell growth and replication of M dsRNAvirus; contains four beta-transducin repeats 0.006650.06745&radic&radic&radic&radic S000000999STE20YHL007CverifiedSignal transducing kinase of the PAK (p21-activated kinase)family, involved in pheromone response andpseudohyphal/invasive growth pathways,activated by Cdc42p; binds Ste4p at a GBB motifpresent in noncatalytic domains of PAK kinases 0.006640.06745&radic&radic&radic S000005976LGE1YPL055CverifiedProtein of unknown function; null mutant forms abnormally largecells 0.006630.06745&radic&radic&radic&radic&radic&radic S000005867RPA43YOR340CverifiedRNA polymerase I subunit A43 0.006630.06745&radic&radic S000002352NUS1YDL193WuncharacterizedPrenyltransferase, required for cell viability; involved inprotein trafficking 0.006640.06745 S000001754PET10YKR046CuncharacterizedProtein of unknown function that co-purifies with lipidparticles; expression pattern suggests a rolein respiratory growth; computational analysisof large-scale protein-protein interaction datasuggests a role in ATP/ADP exchange 0.006680.06745&radic S000002583NGG1YDR176WverifiedTranscriptional regulator involved in glucose repression ofGal4p-regulated genes; component oftranscriptional adaptor and histoneacetyltransferase complexes, the ADA complex,the SAGA complex, and the SLIK complex 0.006640.06745&radic&radic S000001591SLD2YKL108WverifiedProtein required for DNA replication, phosphorylated in S phaseby S-phase cyclin-dependent kinases (Cdks),phosphorylation is essential for DNAreplication and for complex formation withDpb11p; potential Cdc28p substrate 0.006650.06745&radic&radic&radic S000000607POL4YCR014CverifiedDNA polymerase IV, undergoes pair-wise interactions withDnl4p-Lif1p and Rad27p to mediate repair of DNAdouble-strand breaks by non-homologous endjoining (NHEJ); homologous to mammalian DNApolymerase beta 0.006610.06726 S000005924ULA1YPL003WverifiedProtein that acts together with Uba3p to activate Rub1p beforeits conjugation to proteins (neddylation),which may play a role in protein degradation 0.006610.06726&radic&radic&radic&radic&radic&radic S000005778TMA16YOR252WverifiedProtein of unknown function that associates with ribosomes 0.006610.06665&radic&radic&radic S000001259IMD2YHR216WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, expression is induced bymycophenolic acid resulting in resistance tothe drug, expression is repressed by nutrientlimitation 0.006590.06665&radic&radic&radic S000000752SNU13YEL026WverifiedRNA binding protein, part of U3 snoRNP involved in rRNAprocessing, part of U4/U6-U5 tri-snRNP involvedin mRNA splicing, similar to human 15.5Kprotein 0.006590.06665&radic&radic&radic S000000929LCP5YER127WverifiedEssential protein involved in maturation of 18S rRNA; depletionleads to inhibited pre-rRNA processing andreduced polysome levels; localizes primarily tothe nucleolus 0.00660.06665 S000004829SKY1YMR216CverifiedSR protein kinase (SRPK) involved in regulating proteinsinvolved in mRNA metabolism and cationhomeostasis; similar to human SRPK1 0.00660.06665&radic&radic&radic&radic S000005524BDS1YOL164WverifiedBacterially-derived sulfatase required for use of alkyl- andaryl-sulfates as sulfur sources 0.00660.06665 S000001849SEC53YFL045CverifiedPhosphomannomutase, involved in synthesis of GDP-mannose anddolichol-phosphate-mannose; required forfolding and glycosylation of secretory proteinsin the ER lumen 0.006610.06665&radic&radic&radic&radic&radic&radic S000005475PAP2YOL115WverifiedCatalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), anuclear poly (A) polymerase complex involved inRNA quality control; catalyzes polyadenylationof unmodified tRNAs, and snoRNA and rRNAprecursors; disputed role as a DNA polymerase 0.006570.06596 S000002386HOYDL227CverifiedSite-specific endonuclease required for gene conversion at theMAT locus (homothallic switching) through thegeneration of a ds DNA break; expressionrestricted to mother cells in late G1 ascontrolled by Swi4p-Swi6p, Swi5p and Ash1p 0.006570.06596 S000004410CDC73YLR418CverifiedConstituent of Paf1 complex with RNA polymerase II, Paf1p,Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinctfrom Srb-containing Pol II complexes; requiredfor expression of certain genes, modificationof some histones, and telomere maintenance 0.006560.06596&radic&radic&radic&radic&radic S000000028SNC1YAL030WverifiedVesicle membrane receptor protein (v-SNARE) involved in thefusion between Golgi-derived secretory vesicleswith the plasma membrane; proposed to beinvolved in endocytosis; member of thesynaptobrevin/VAMP family of R-type v-SNAREproteins 0.006580.06596&radic&radic&radic&radic&radic S000003432ELP2YGR200CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;target of Kluyveromyces lactis zymocin 0.006550.06576&radic&radic&radic&radic&radic S000001564TEF4YKL081WverifiedTranslation elongation factor EF-1 gamma 0.006550.06576&radic&radic&radic&radic&radic S000002990STT3YGL022WverifiedSubunit of the oligosaccharyltransferase complex of the ERlumen, which catalyzes asparagine-linkedglycosylation of newly synthesized proteins;forms a subcomplex with Ost3p and Ost4p and isdirectly involved in catalysis 0.006550.06576&radic&radic&radic&radic S000003674TIF2YJL138CverifiedTranslation initiation factor eIF4A, identical to Tif1p;DEA(D/H)-box RNA helicase that couples ATPaseactivity to RNA binding and unwinding; forms adumbbell structure of two compact domainsconnected by a linker; interacts with eIF4G 0.006550.06576&radic&radic&radic S000000364CDC28YBR160WverifiedCatalytic subunit of the main cell cycle cyclin-dependentkinase (CDK); alternately associates with G1cyclins (CLNs) and G2/M cyclins (CLBs) whichdirect the CDK to specific substrates 0.00650.06485&radic S000005585YOR059CUncharacterizedHypothetical protein 0.006540.06485&radic&radic&radic&radic S000004920UBP15YMR304WverifiedUbiquitin-specific protease that may play a role in ubiquitinprecursor processing 0.006530.06485&radic&radic&radic&radic&radic&radic S000000122LSM2YBL026WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.006540.06485&radic&radic&radic&radic&radic S000002780VPS74YDR372CverifiedNon-essential protein of unknown function involved in vacuolarprotein sorting; belongs to a family ofcytosolic Golgi-associated proteins suggestingthat it may play a role in secretion; alsodetected in the nucleus 0.006510.06485&radic&radic&radic&radic&radic&radic S000005372HTZ1YOL012CverifiedHistone variant H2AZ, exchanged for histone H2A in nucleosomesby the SWR1 complex; involved intranscriptional regulation through preventionof the spread of silent heterochromatin 0.006540.06485&radic&radic&radic&radic S000002577SEC7YDR170CverifiedGuanine nucleotide exchange factor (GEF) for ADP ribosylationfactors involved in proliferation of the Golgi,intra-Golgi transport and ER-to-Golgitransport; found in the cytoplasm and onGolgi-associated coated vesicles 0.006510.06485&radic&radic&radic S000001618APL2YKL135CverifiedBeta-adaptin, large subunit of the clathrin-associated protein(AP-1) complex; binds clathrin; involved inclathrin-dependent Golgi protein sorting 0.006530.06485&radic&radic S000000488YBR284WUncharacterizedPutative protein of unknown function; YBR284W is not an essential gene; null mutant exhibits decreased resistance to rapamycin and wortmannin 0.006460.06369 S000005785RPT4YOR259CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for spindlepole body duplication; localized mainly to thenucleus throughout the cell cycle 0.006480.06369&radic&radic&radic&radic S000003628HPR5YJL092WverifiedDNA helicase and DNA-dependent ATPase involved in DNA repair,required for proper timing of commitment tomeiotic recombination and the transition fromMeiosis I to Meiosis II; potential Cdc28psubstrate 0.006490.06369 S000000885GET2YER083CverifiedSubunit of the GET complex; required for meiotic nucleardivision and for the retrieval of HDEL proteinsfrom the Golgi to the ER in an ERD2 dependentfashion; may be involved in cell wall function 0.006470.06369&radic S000002515GSG1YDR108WverifiedSubunit of TRAPP (transport protein particle), a multi-subunitcomplex involved in targeting and/or fusion ofER-to-Golgi transport vesicles with theiracceptor compartment; protein has late meioticrole, following DNA replication 0.006440.06369&radic&radic&radic S000000118PIM1YBL022CverifiedMitochondrial ATP-dependent protease involved inintramitochondrial proteolysis; involved indegradation of misfolded proteins inmitochondria; required for bigenesis andmaintenance of mitochondria 0.006480.06369&radic&radic&radic S000002769BCP1YDR361CverifiedEssential protein involved in nuclear export of Mss4p, which isa lipid kinase that generatesphosphatidylinositol 4,5-biphosphate and playsa role in actin cytoskeleton organization andvesicular transport 0.006450.06369&radic&radic&radic&radic S000004465GIS4YML006CverifiedCAAX box containing protein of unknown function, proposed to beinvolved in the RAS/cAMP signaling pathway 0.00650.06369&radic&radic&radic&radic&radic S000006125HRR25YPL204WverifiedProtein kinase involved in regulating diverse events includingvesicular trafficking, gene expression, DNArepair, and chromosome segregation; binds theCTD of RNA pol II; homolog of mammalian caseinkinase 1delta (CK1delta) 0.006460.06369&radic&radic&radic&radic&radic S000000816HEM14YER014WverifiedProtoporphyrinogen oxidase, a mitochondrial enzyme thatcatalyzes the seventh step in the hemebiosynthetic pathway, convertingprotoporphyrinogen IX to protoporphyrin IX 0.006470.06369&radic&radic&radic&radic S000001918ROG3YFR022WverifiedProtein that binds to Rsp5p, which is a hect-type ubiquitinligase, via its 2 PY motifs; has similarity toRod1p; mutation suppresses the temperaturesensitivity of an mck1 rim11 double mutant 0.006460.06369 S000003700TPK1YJL164CverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; inhibits filamentous growth 0.006460.06369&radic&radic&radic&radic&radic S000005187SLA2YNL243WverifiedTransmembrane actin-binding protein involved in membranecytoskeleton assembly and cell polarization;adaptor protein that links actin to clathrinand endocytosis; present in the actin corticalpatch of the emerging bud tip; dimer in vivo 0.006480.06369&radic S000005713TUF1YOR187WverifiedMitochondrial translation elongation factor Tu; comprises bothGTPase and guanine nucleotide exchange factoractivities, while these activities are found inseparate proteins in S. pombe and humans 0.006470.06369&radic&radic S000002732UTP4YDR324CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.006450.06369&radic S000003849BIR1YJR089WverifiedEssential chromosomal passenger protein involved incoordinating cell cycle events for properchromosome segregation; C-terminal region bindsSli15p, and the middle region, uponphosphorylation, localizes Cbf2p to the spindleat anaphase 0.006430.06337 S000004433RPS1AYLR441CverifiedRibosomal protein 10 (rp10) of the small (40S) subunit; nearlyidentical to Rps1Bp and has similarity to ratS3a ribosomal protein 0.006440.06337&radic&radic&radic S000003618IML2YJL082WverifiedProtein of unknown function; the authentic, non-tagged proteinis detected in highly purified mitochondria inhigh-throughput studies 0.006430.06337&radic&radic S000005394SMC5YOL034WverifiedStructural maintenance of chromosomes (SMC) protein; essentialsubunit of the Mms21-Smc5-Smc6 complex;required for growth and DNA repair; S. pombehomolog forms a heterodimer with S. pombeRad18p that is involved in DNA repair 0.006430.06337&radic&radic&radic&radic S000005233PCL1YNL289WverifiedPho85 cyclin of the Pcl1,2-like subfamily, involved in entryinto the mitotic cell cycle and regulation ofmorphogenesis, localizes to sites of polarizedcell growth 0.006440.06337&radic S000003593IKS1YJL057CuncharacterizedPutative serine/threonine kinase; expression is induced duringmild heat stress; deletion mutants arehypersensitive to copper sulphate and resistantto sorbate; interacts with an N-terminalfragment of Sst2p 0.006430.06337&radic&radic S000000229YBR025CUncharacterizedHypothetical protein 0.006430.06337&radic&radic S000000301VPS15YBR097WverifiedMyristoylated serine/threonine protein kinase involved invacuolar protein sorting; functions as amembrane-associated complex with Vps34p; activeform recruits Vps34p to the Golgi membrane;interacts with the GDP-bound form of Gpa1p 0.006430.06337 S000004848RNA1YMR235CverifiedGTPase activating protein (GAP) for Gsp1p, involved in nucleartransport 0.006420.06283&radic&radic&radic&radic S000007255TIM11YDR322C-AverifiedSubunit e of mitochondrial F1F0-ATPase, which is a large,evolutionarily conserved enzyme complexrequired for ATP synthesis; essential for thedimeric state of ATP synthase 0.006410.06283 S000001016RIM4YHL024WverifiedPutative RNA-binding protein required for the expression ofearly and middle sporulation genes 0.00640.06283&radic S000000963SPT2YER161CverifiedProtein involved in negative regulation of transcription;required for RNA polyadenylation; exhibitsregulated interactions with both histones andSWI-SNF components, has similarity to mammalianHMG1 proteins 0.006420.06283 S000005216SEC2YNL272CverifiedGuanyl-nucleotide exchange factor for the small G-proteinSec4p, located on cytoplasmic vesicles;essential for post-Golgi vesicle transport 0.006410.06283&radic&radic&radic&radic&radic&radic S000001910CMK1YFR014CverifiedCalmodulin-dependent protein kinase; may play a role in stressresponse, many CA++/calmodulan dependentphosphorylation substrates demonstrated invitro, amino acid sequence similar to Cmk2p andmammalian Cam Kinase II 0.00640.06283&radic&radic S000002338PCL9YDL179WverifiedCyclin, forms a functional kinase complex with Pho85pcyclin-dependent kinase (Cdk), expressed inlate M/early G1 phase, activated by Swi5p 0.006420.06283 S000006034YPL113CUncharacterizedPutative dehydrogenase 0.006420.06283&radic&radic&radic S000003420BUB1YGR188CverifiedProtein kinase that forms a complex with Mad1p and Bub3p thatis crucial in the checkpoint mechanism requiredto prevent cell cycle progression into anaphasein the presence of spindle damage, associateswith centromere DNA via Skp1p 0.00640.06283 S000002261QRI1YDL103CverifiedUDP-N-acetylglucosamine pyrophosphorylase, catalyzes theformation of UDP-N-acetylglucosamine(UDP-GlcNAc), which is important in cell wallbiosynthesis, protein N-glycosylation, and GPIanchor biosynthesis 0.006420.06283&radic S000001891MSH4YFL003CverifiedProtein involved in meiotic recombination, required for normallevels of crossing over, colocalizes with Zip2pto discrete foci on meiotic chromosomes, hashomology to bacterial MutS protein 0.006410.06283 S000000103SLA1YBL007CverifiedCytoskeletal protein binding protein required for assembly ofthe cortical actin cytoskeleton; contains 3 SH3domains; interacts with proteins regulatingactin dynamics and with proteins required forendocytosis 0.006390.06272&radic&radic&radic&radic S000006032CAR1YPL111WverifiedArginase, responsible for arginine degradation, expressionresponds to both induction by arginine andnitrogen catabolite repression; disruptionenhances freeze tolerance 0.006380.06264&radic&radic&radic&radic&radic&radic&radic S000004378VAC14YLR386WverifiedProtein involved in regulated synthesis of PtdIns(3,5)P(2), incontrol of trafficking of some proteins to thevacuole lumen via the MVB, and in maintenanceof vacuole size and acidity; activator of Fab1p 0.006360.06236&radic&radic&radic&radic S000002693ZIP1YDR285WverifiedTransverse filament protein of the synaptonemal complex;required for normal levels of meioticrecombination and pairing between homologouschromosome during meiosis; potential Cdc28psubstrate 0.006370.06236&radic&radic S000000400PGI1YBR196CverifiedGlycolytic enzyme phosphoglucose isomerase, catalyzes theinterconversion of glucose-6-phosphate andfructose-6-phosphate; required for cell cycleprogression and completion of the gluconeogenicevents of sporulation 0.006360.0623&radic&radic&radic S000000849SAP1YER047CverifiedPutative ATPase of the AAA family, interacts with the Sin1ptranscriptional repressor in the two-hybridsystem 0.006360.0623&radic&radic&radic&radic&radic&radic&radic S000001948RPN12YFR052WverifiedSubunit of the 19S regulatory particle of the 26S proteasomelid; synthetically lethal with RPT1, which isan ATPase component of the 19S regulatoryparticle; physically interacts with Nob1p andRpn3p 0.006350.06188&radic&radic&radic&radic S000005194RAD50YNL250WverifiedSubunit of MRX complex, with Mre11p and Xrs2p, involved inprocessing double-strand DNA breaks invegetative cells, initiation of meiotic DSBs,telomere maintenance, and nonhomologous endjoining 0.006350.06188&radic S000002921GRX2YDR513WverifiedCytoplasmic glutaredoxin, thioltransferase,glutathione-dependent disulfide oxidoreductaseinvolved in maintaining redox state of targetproteins, also exhibits glutathione peroxidaseactivity, expression induced in response tostress 0.006360.06188&radic S000004322CHS5YLR330WverifiedProtein involved in export from the Golgi to plasma membrane;involved in chitin biosynthesis through itsrole in Chs3p localization; interacts withArf1p, Bch1p, Fmp50p, Bud7p, and Chs6p 0.006350.06188&radic&radic S000003957RIX7YLL034CverifiedPutative ATPase of the AAA family, required for export ofpre-ribosomal large subunits from the nucleus;distributed between the nucleolus, nucleoplasm,and nuclear periphery depending on growthconditions 0.006350.06188&radic&radic&radic&radic S000000777VMA8YEL051WverifiedSubunit D of the eight-subunit V1 peripheral membrane domain ofthe vacuolar H+-ATPase (V-ATPase), anelectrogenic proton pump found throughout theendomembrane system; plays a role in thecoupling of proton transport and ATP hydrolysis 0.006360.06188&radic&radic&radic&radic&radic&radic S000003642IME2YJL106WverifiedSerine/threonine protein kinase involved in activation ofmeiosis, associates with Ime1p and mediates itsstability, activates Ndt80p; IME2 expression ispositively regulated by Ime1p 0.006350.06188 S000002344TFP1YDL185WverifiedVacuolar ATPase V1 domain subunit A containing the catalyticnucleotide binding sites; protein precursorundergoes self-catalyzed splicing to yield theextein Tfp1p and the intein Vde (PI-SceI),which is a site-specific endonuclease 0.006340.06188&radic S000006137YPL216WUncharacterizedHypothetical protein 0.006310.06149&radic&radic&radic&radic&radic S000006365SGV1YPR161CverifiedCyclin (Bur2p)-dependent protein kinase that functions intranscriptional regulation; phosphorylates thecarboxy-terminal domain of Rpo21p, which is thelargest subunit of RNA polymerase II; regulatedby Cak1p 0.006320.06149 S000002319DHH1YDL160CverifiedCytoplasmic DExD/H-box helicase, stimulates mRNA decapping,coordinates distinct steps in mRNA function anddecay, interacts with both the decapping anddeadenylase complexes, may have a role in mRNAexport and translation 0.006320.06149&radic&radic S000006127YPL206CUncharacterizedProtein that plays a role in metabolism of glycerophosphocholine to phosphatidylcholine; contains glycerophosphodiester phosphodiesterase motifs 0.006320.06149&radic S000003616SCP160YJL080CverifiedEssential RNA-binding G protein effector of mating responsepathway, predominantly associated with nuclearenvelope and ER, interacts in mRNA-dependentmanner with translating ribosomes via multipleKH domains, similar to vertebrate vigilins 0.00630.06149&radic S000006198YPL277CUncharacterizedHypothetical protein 0.006320.06149 S000006369RHO1YPR165WverifiedGTP-binding protein of the rho subfamily of Ras-like proteins,involved in establishment of cell polarity;regulates protein kinase C (Pkc1p) and the cellwall synthesizing enzyme 1,3-beta-glucansynthase (Fks1p and Gsc2p) 0.006280.06104&radic&radic S000004640MSN2YMR037CverifiedTranscriptional activator related to Msn4p; activated in stressconditions, which results in translocation fromthe cytoplasm to the nucleus; binds DNA atstress response elements of responsive genes,inducing gene expression 0.006270.06104&radic S000001672HYM1YKL189WverifiedComponent of the RAM signaling network that is involved inregulation of Ace2p activity and cellularmorphogenesis, interacts with Kic1p and Sog2p,localizes to sites of polarized growth duringbudding and during the mating response 0.00630.06104&radic&radic&radic&radic&radic S000001134HXT4YHR092CverifiedHigh-affinity glucose transporter of the major facilitatorsuperfamily, expression is induced by lowlevels of glucose and repressed by high levelsof glucose 0.006290.06104&radic&radic&radic S000005405PSK2YOL045WverifiedOne of two (see also PSK1) PAS domain containing S/T proteinkinases; regulates sugar flux and translationin response to an unknown metabolite byphosphorylating Ugp1p and Gsy2p (sugar flux)and Caf20p, Tif11p and Sro9p (translation) 0.006270.06104&radic&radic&radic&radic&radic&radic&radic S000005670ELG1YOR144CverifiedProtein required for S phase progression and telomerehomeostasis, forms an alternative replicationfactor C complex important for DNA replicationand genome integrity; involved in homologousrecombination-mediated DNA repair 0.006290.06104&radic&radic&radic S000000653PWP2YCR057CverifiedConserved 90S pre-ribosomal component essential for properendonucleolytic cleavage of the 35 S rRNAprecursor at A0, A1, and A2 sites; containseight WD-repeats; PWP2 deletion leads todefects in cell cycle and bud morphogenesis 0.006290.06104 S000003358YGR126WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 0.006270.06104&radic&radic S000005005NOP2YNL061WverifiedProbable RNA m(5)C methyltransferase, essential for processingand maturation of 27S pre-rRNA and largeribosomal subunit biogenesis; localized to thenucleolus; constituent of 66S pre-ribosomalparticles 0.00630.06104 S000004925PSE1YMR308CverifiedKaryopherin/importin that interacts with the nuclear porecomplex; acts as the nuclear import receptorfor specific proteins, including Pdr1p, Yap1p,Ste12p, and Aft1p 0.006280.06104&radic S000002289LYS21YDL131WverifiedHomocitrate synthase isozyme, catalyzes the condensation ofacetyl-CoA and alpha-ketoglutarate to formhomocitrate, which is the first step in thelysine biosynthesis pathway; highly similar tothe other isozyme, Lys20p 0.00630.06104 S000002586CSN9YDR179CverifiedSubunit of the Cop9 signalosome, which is required fordeneddylation, or removal of the ubiquitin-likeprotein Rub1p from Cdc53p (cullin); involved inadaptation to pheromone signaling 0.006290.06104&radic&radic&radic&radic S000003335NOP7YGR103WverifiedNucleolar protein involved in rRNA processing and 60S ribosomalsubunit biogenesis; constituent of severaldifferent pre-ribosomal particles; required forexit from G0 and the initiation ofcell proliferation 0.006260.06045&radic&radic&radic&radic S000006256NHP6AYPR052CverifiedHigh-mobility group non-histone chromatin protein, functionallyredundant with Nhp6Bp; homologous to mammalianhigh mobility group proteins 1 and 2; acts torecruit transcription factor Rcs1p to certainpromoters 0.006250.06045&radic S000004266DBP9YLR276CverifiedATP-dependent RNA helicas