Yfunc lets you browse current and predicted gene functions in yeast

Enter a GO term ID (example: GO:0003723):
Enter a yeast gene name to browse (example:AIR1):

Prediction of GO:0019787 "small conjugating protein ligase activity"

GO branch: MF
Number of currently annotated genes: 38

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest TAP:69Huh:nucleusHarbison:MSN2_HEATLum:FUDRLum:ChlorpromazineLum:TunicamycinLee:Gcn5 ypdPF00646Harbison:IME1_YPDPF08238 S000004117APC2YLR127CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; similar tocullin Cdc53p &radic0.565130.95823&radic&radic&radic&radic S000000927RSP5YER125WverifiedUbiquitin-protein ligase involved in ubiquitin-mediated proteindegradation; plays a role in heat shock element(HSE)-mediated gene expression andmultivesicular body sorting; contains a hect(homologous to E6-AP carboxyl terminus) domain &radic0.529480.95774 S000002290CDC53YDL132WverifiedCullin, structural protein of SCF complexes (which also containSkp1p, Cdc34p, and an F-box protein) involvedin ubiquitination; SCF promotes the G1-Stransition by targeting G1 cyclins and theCln-CDK inhibitor Sic1p for degradation &radic0.520470.95196&radic S000003209DOC1YGL240WverifiedProcessivity factor required for the ubiquitination activity ofthe anaphase promoting complex (APC), mediatesthe activity of the APC by contributing tosubstrate recognition; involved in cyclinproteolysis 0.461030.93469&radic&radic S000003583RTT101YJL047CverifiedCullin subunit of a Roc1p-dependent E3 ubiquitin ligasecomplex; deletion phenotype suggests a role inanaphase progression; interacts with Mms22p andimplicated in Mms22-dependent DNA repair;modified by the ubiquitin-like protein, Rub1p &radic0.404590.93469&radic&radic&radic S000002817SIZ1YDR409WverifiedSUMO ligase that promotes the attachment of sumo (Smt3p; smallubiquitin-related modifier) to proteins; bindsUbc9p and may bind septins; specificallyrequired for sumoylation of septins in vivo;localized to the septin ring &radic0.344970.92111&radic&radic&radic&radic S000001505CDC16YKL022CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; required forsporulation &radic0.336020.91547&radic&radic&radic&radic&radic&radic&radic S000000180CDC27YBL084CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.330850.91207&radic&radic&radic&radic&radic S000005682NFI1YOR156CverifiedSUMO ligase, catalyzes the covalent attachment of SUMO (Smt3p)to proteins; involved in maintenance of propertelomere length &radic0.311950.89937&radic&radic&radic&radic&radic S000001209CDC23YHR166CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.297510.89085&radic&radic S000002865TOM1YDR457WverifiedE3 ubiquitin ligase of the hect-domain class; has a role inmRNA export from the nucleus and may regulatetranscriptional coactivators &radic0.266480.88135&radic&radic&radic S000003797HUL4YJR036CverifiedProtein with similarity to hect domain E3 ubiquitin-proteinligases, not essential for viability &radic0.258130.87702&radic S000003109HUL5YGL141WverifiedProtein with similarity to hect domain E3 ubiquitin-proteinligases, not essential for viability &radic0.254170.87656&radic S000003235CUL3YGR003WverifiedUbiquitin-protein ligase, member of the cullin family withsimilarity to Cdc53p and human CUL3; nullmutation has no apparent phenotype &radic0.246660.86937&radic&radic S000001493UFD4YKL010CverifiedUbiquitin-protein ligase (E3) that interacts with Rpt4p andRpt6p, two subunits of the 19S particle of the26S proteasome; cytoplasmic E3 involved in thedegradation of ubiquitin fusion proteins &radic0.237710.86118&radic&radic&radic&radic&radic S000005116APC1YNL172WverifiedLargest subunit of the Anaphase-Promoting Complex/Cyclosome(APC/C), which is a ubiquitin-protein ligaserequired for degradation of anaphaseinhibitors, including mitotic cyclins, duringthe metaphase/anaphase transition &radic0.227180.85411&radic&radic&radic&radic S000001855ACT1YFL039CverifiedActin, structural protein involved in cell polarization,endocytosis, and other cytoskeletal functions 0.220040.84763&radic&radic S000000745MMS21YEL019CverifiedSUMO ligase involved in chromosomal organization and DNArepair; essential subunit of theMms21-Smc5-Smc6 complex; mutants are sensitiveto methyl methanesulfonate and show increasedspontaneous mutation and mitotic recombination &radic0.146750.76226&radic&radic S000005373HRD1YOL013CverifiedUbiquitin-protein ligase required for endoplasmicreticulum-associated degradation (ERAD) ofmisfolded proteins; genetically linked to theunfolded protein response (UPR); regulatedthrough association with Hrd3p; contains an H2ring finger &radic0.144140.75816&radic&radic S000003685YJL149WVerifiedPutative SCF ubiquitin ligase F-box protein of unknown function; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C; YJL149W is a non-essential gene 0.140090.75265&radic&radic&radic&radic S000002166APC11YDL008WverifiedCatalytic core subunit of the Anaphase-PromotingComplex/Cyclosome (APC/C), which is aubiquitin-protein ligase required fordegradation of anaphase inhibitors, includingmitotic cyclins, during the metaphase/anaphasetransition &radic0.121510.72315&radic S000005606DIA2YOR080WverifiedOrigin-binding F-box protein that forms an SCF ubiquitin ligasecomplex with Skp1p and Cdc53p; plays a role inDNA replication, involved in invasive andpseudohyphal growth 0.120540.72036&radic&radic S000004360MDM30YLR368WverifiedF-box protein; physically associates with mitochondria and isrequired for normal mitochondrial fusion inrich medium, during sporulation, and in matingcells; promotes ubiquitin-mediated degradationof Gal4p in some strains 0.119590.71781&radic&radic&radic S000005255SKP2YNL311CverifiedPutative F-box protein; analysis of integrated high-throughputdatasets predicts an involvement inubiquitin-dependent protein catabolism 0.118820.71682&radic&radic S000005493HRT1YOL133WverifiedRING finger containing subunit of Skp1-Cullin-F-box ubiquitinprotein ligases (SCF); required for Gic2p,Far1p, Sic1p and Cln2p degradation; may tetherCdc34p (a ubiquitin conjugating enzyme or E2)and Cdc53p (a cullin) subunits of SCF &radic0.117310.71405&radic S000001308MET30YIL046WverifiedF-box protein containing five copies of the WD40 motif,controls cell cycle function, sulfurmetabolism, and methionine biosynthesis as partof the ubiquitin ligase complex; interacts withand regulates Met4p, localizes within thenucleus 0.116310.71186&radic&radic&radic&radic&radic S000003416UBR1YGR184CverifiedUbiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2pto ubiquitinate substrates of the N-end rulepathway; binds to the Rpn2p, Rpt1p, and Rpt6pproteins of the 19S particle of the 26Sproteasome &radic0.111440.70262&radic&radic&radic S000000662RAD18YCR066WverifiedProtein involved in postreplication repair; bindssingle-stranded DNA and has single-stranded DNAdependent ATPase activity; forms heterodimerwith Rad6p; contains RING-finger motif 0.111420.70251 S000003740RCY1YJL204CverifiedF-box protein involved in recycling plasma membrane proteinsinternalized by endocytosis; localized to sitesof polarized growth 0.11080.70101&radic&radic&radic&radic S000004014UBR2YLR024CverifiedCytoplasmic ubiquitin-protein ligase (E3) &radic0.106660.69383&radic&radic&radic S000004022RAD5YLR032WverifiedSingle-stranded DNA-dependent ATPase, involved inpostreplication repair; contains RING fingerdomain 0.105650.69172&radic&radic S000004087HRT3YLR097CuncharacterizedPutative nuclear ubiquitin ligase, based on computationalanalysis of large-scale protein-proteininteraction data; has similarity to F-boxproteins; identified in association withCdc53p, Skp1p and Ubi4 in large-scale studies 0.093650.66793&radic&radic&radic&radic S000001517TUL1YKL034WverifiedGolgi-localized RING-finger ubiquitin ligase (E3), involved inubiquitinating and sorting membrane proteinsthat contain polar transmembrane domains tomultivesicular bodies for delivery to thevacuole for quality control purposes &radic0.093450.66737&radic&radic&radic S000004553UFO1YML088WverifiedF-box receptor protein, subunit of the Skp1-Cdc53-F-boxreceptor (SCF) E3 ubiquitin ligase complex;binds to phosphorylated Ho endonuclease,allowing its ubiquitylation by SCF andsubsequent degradation &radic0.089050.65431&radic S000002362ACK1YDL203CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.086020.64503&radic S000004138PEP3YLR148WverifiedVacuolar peripheral membrane protein that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, required for vacuolarbiogenesis, forms complex with Pep5p thatmediates protein transport to the vacuole 0.084810.64205&radic&radic&radic&radic S000004861RKR1YMR247CverifiedProtein of unknown function; may interact with ribosomes, basedon co-purification experiments; YMR247C is notan essential gene 0.077730.61893&radic&radic S000005967ELC1YPL046CverifiedElongin C, forms heterodimer with Ela1p that participates intranscription elongation; expressiondramatically upregulated during sporulation;widely conserved among eukaryotes 0.077120.61699&radic&radic&radic&radic S000004237IRC20YLR247CuncharacterizedPutative helicase; localized to mitochondria and the nucleus;YLR247C is not an essential gene; null mutantdisplays increased levels of spontaneous Rad52foci 0.073830.60657&radic&radic&radic&radic S000000407COS111YBR203WverifiedProtein required for resistance to the antifungal drugciclopirox olamine; not related to thesubtelomerically-encoded COS family; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 0.073310.60549&radic&radic S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.073340.60549&radic&radic&radic S000002673PEX10YDR265WverifiedRING finger peroxisomal membrane peroxin required forperoxisomal matrix protein import, interactswith Pex12p, links ubiquitin-conjugating Pex4pto protein import machinery; mutations in humanhomolog cause a variety of peroxisomaldisorders 0.072890.60388&radic&radic S000000918SLX8YER116CverifiedProtein containing a RING finger domain that interacts withHex3p; mutant phenotypes and geneticinteractions suggest a role in sumoylation andin genome stability 0.07270.60273&radic S000004315CWC24YLR323CverifiedEssential protein, component of a complex containing Cef1p; hassimilarity to S. pombe Cwf24p 0.071090.59629&radic&radic&radic&radic S000004844PEP5YMR231WverifiedPeripheral vacuolar membrane protein required for proteintrafficking and vacuole biogenesis; formscomplex with Pep3p that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, also interacts with Pep7p 0.068620.58883&radic&radic&radic S000005583SGT1YOR057WverifiedProbable cochaperone, regulates activity of Cyr1p (adenylylcyclase); involved in assembly of thekinetochore complex, associates with the SCF(Skp1p/Cdc53p/F box protein) ubiquitin ligasecomplex 0.068150.58553&radic&radic&radic&radic S000005415PSH1YOL054WuncharacterizedNuclear protein, putative RNA polymerase II elongation factor;isolated as Pob3p/Spt16p-binding protein 0.068360.58553&radic&radic&radic S000005498YOL138CUncharacterizedHypothetical protein 0.067580.58422 S000000002VPS8YAL002WverifiedMembrane-associated hydrophilic protein that interacts with thesmall GTPase, Vps21p, to facilitate solublevacuolar protein localization; required forlocalization and trafficking of the CPY sortingreceptor; contains a RING finger motif 0.065960.57911&radic&radic S000002627MFB1YDR219CverifiedMitochondria-associated F-box protein involved in maintenanceof normal mitochondrial morphology; interactswith Skp1p through the F-box motif;preferentially localizes to the mother cellduring budding 0.065690.57842&radic&radic&radic&radic&radic&radic S000004968FAP1YNL023CverifiedProtein that binds to Fpr1p (FKBP12), conferring rapamycinresistance by competing with rapamycin forFpr1p binding; has similarity to putativetranscription factors, including D.melanogaster shuttle craft and human NFX1 0.064860.57507&radic&radic S000003850GRR1YJR090CverifiedF-box protein component of the SCF ubiquitin-ligase complex,required for Cln1p and Cln2p degradation;involved in carbon catabolite repression,glucose-dependent divalent cation transport,high-affinity glucose transport, andmorphogenesis &radic0.064120.57161&radic S000001885CDC4YFL009WverifiedF-box protein required for G1/S and G2/M transition, associateswith Skp1p and Cdc53p to form a complex,SCFCdc4, which acts as ubiquitin-protein ligasedirecting ubiquitination of the phosphorylatedCDK inhibitor Sic1p &radic0.064020.57159&radic&radic&radic&radic S000001002YHL010CUncharacterizedPutative protein of unknown function, contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences 0.063060.56763&radic S000004419MAG2YLR427WuncharacterizedDNA-3-methyladenine glycosidase II that catalyzes of thehydrolysis of alkylated DNA 0.060590.55781 S000001157DMA1YHR115CverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma2p;homolog of S. pombe Dma1 and H. sapiens Chfr 0.060.55642&radic S000002674YDR266CUncharacterizedHypothetical protein 0.058430.54941&radic&radic S000000318RAD16YBR114WverifiedProtein that recognizes and binds damaged DNA in anATP-dependent manner (with Rad7p) duringnucleotide excision repair; subunit ofNucleotide Excision Repair Factor 4 (NEF4);member of the SWI/SNF family 0.055970.53933&radic S000000266YBR062CUncharacterizedHypothetical protein 0.054160.53059 S000002721PIB1YDR313CverifiedRING-type ubiquitin ligase of the endosomal and vacuolarmembranes, bindsphosphatidylinositol(3)-phosphate; contains aFYVE finger domain &radic0.053920.52874&radic&radic S000006297ASR1YPR093CverifiedProtein involved in a putative alcohol-responsive signalingpathway; accumulates in the nucleus underalcohol stress; contains a Ring/PHD fingerdomain 0.05310.52294&radic S000000870MOT2YER068WverifiedComponent of the CCR4-NOT complex, which has multiple roles intranscription regulation, mRNA degradation, andpost-transcriptional modifications; with Ubc4p,ubiquitylates subunits of the nascentpolypeptide-associated complex (NAC) 0.051210.51611&radic&radic S000004533ITT1YML068WverifiedProtein that modulates the efficiency of translationtermination, interacts with translation releasefactors eRF1 (Sup45p) and eRF3 (Sup35p) invitro, contains a zinc finger domaincharacteristic of the TRIAD class of proteins 0.051010.51355 S000000484SAF1YBR280CverifiedF-Box protein involved in proteasome-dependent degradation ofAah1p during entry of cells into quiescence;interacts with Skp1 0.050340.5105&radic S000001411MLP2YIL149CverifiedMyosin-like protein associated with the nuclear envelope,connects the nuclear pore complex with thenuclear interior; involved in the Tel1p pathwaythat controls telomere length 0.050030.50824 S000003746PEX2YJL210WverifiedRING-finger peroxin, peroxisomal membrane protein with aC-terminal zinc-binding RING domain, formsputative translocation subcomplex with Pex10pand Pex12p which functions in peroxisomalmatrix protein import 0.04960.50576&radic S000002550SAN1YDR143CverifiedUbiquitin-protein ligase, involved in the proteasome-dependentdegradation of aberrant nuclear proteins; san1mutations suppress sir4, spt16, and cdc68mutations, suggesting a role in chromatinsilencing &radic0.049020.50298&radic&radic S000000157SKT5YBL061CverifiedActivator of Chs3p (chitin synthase III), recruits Chs3p to thebud neck via interaction with Bni4p; hassimilarity to Shc1p, which activates Chs3pduring sporulation 0.048720.50209&radic&radic&radic S000000898SHC1YER096WverifiedSporulation-specific activator of Chs3p (chitin synthase III),required for the synthesis of the chitosanlayer of ascospores; has similarity to Skt5p,which activates Chs3p during vegetative growth;transcriptionally induced at alkaline pH 0.048590.50024&radic S000002868TFB3YDR460WverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, involved in transcriptioninitiation, required for nucleotide excisionrepair; ring finger protein similar tomammalian CAK and TFIIH subunit 0.048310.49984&radic S000003693FAR1YJL157CverifiedCyclin-dependent kinase inhibitor that mediates cell cyclearrest in response to pheromone; also forms acomplex with Cdc24p, Ste4p, and Ste18p that mayspecify the direction of polarized growthduring mating; potential Cdc28p substrate 0.048270.49944&radic&radic&radic&radic S000002232BRE1YDL074CverifiedE3 ubiquitin ligase for Rad6p, required for the ubiquitinationof histone H2B, recruitment of Rad6p topromoter chromatin and subsequent methylationof histone H3 (on L4 and L79), contains RINGfinger domain &radic0.046150.49093&radic&radic S000004344YLR352WUncharacterizedPutative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene 0.044110.48165&radic S000005060DMA2YNL116WverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma1p;homolog of S. pombe Dma1 and H. sapiens Chfr 0.04470.48165 S000004665RNA14YMR061WverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; bridges interaction between Rna15p andHrp1p in the CF I complex 0.043190.47713&radic&radic&radic&radic S000000211DSF2YBR007CuncharacterizedDeletion suppressor of mpt5 mutation 0.04290.4771&radic S000006393SKI3YPR189WverifiedProtein involved in exosome mediated 3' to 5' mRNA degradationand translation inhibition of non-poly(A)mRNAs; forms complex with Ski2p and Ski8p;required for repressing propagation of dsRNAviruses 0.042690.47389&radic&radic&radic&radic&radic S000002538YDR131CUncharacterizedF-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex 0.038770.45954&radic&radic&radic&radic S000005257YNL313CUncharacterizedProtein required for cell viability 0.038050.45667&radic&radic S000000865THO1YER063WverifiedProtein of unknown function; overproduction suppresses thetranscriptional defect caused by an hpr1mutation 0.034080.43807&radic S000005553STI1YOR027WverifiedHsp90 cochaperone, interacts with the Ssa group of thecytosolic Hsp70 chaperones; activates theATPase activity of Ssa1p; homolog of mammalianHop protein 0.032430.43037&radic&radic S000004605YMR003WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria; YMR003W is not an essential gene 0.031620.42654&radic S000002714YDR306CUncharacterizedF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain 0.029850.41568&radic&radic&radic S000004628PEX12YMR026CverifiedRING-finger peroxisomal membrane peroxin that plays anessential role in peroxisome biogenesis andperoxisomal matrix protein import, formstranslocation subcomplex with Pex2p and Pex10p 0.029550.41472&radic&radic S000004206CPR6YLR216CverifiedPeptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzesthe cis-trans isomerization of peptide bondsN-terminal to proline residues; binds to Hsp82pand contributes to chaperone activity 0.029240.40929&radic&radic S000004620YMR018WUncharacterizedPutative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene 0.027210.39963&radic&radic&radic S000005533SGT2YOR007CverifiedGlutamine-rich cytoplasmic protein of unknown function;contains tetratricopeptide (TPR) repeats, whichoften mediate protein-protein interactions; hassimilarity to human SGT, which is a cochaperonethat negatively regulates Hsp70 0.026410.39476&radic S000003279TFC4YGR047CverifiedOne of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauA domain of TFIIIC that binds BoxA DNApromoter sites of tRNA and similar genes; hasTPR motifs; human homolog is TFIIIC-102 0.026330.39355&radic&radic&radic&radic S000002510STE5YDR103WverifiedPheromone-responsive scaffold protein; shuttles from thenucleus to the plasma membrane and assembleskinases Ste11p, Ste7p, and Fus3p into aspecific signaling complex; active oligomericform interacts with Ste4p-Ste18p complex 0.026370.39355&radic&radic&radic S000005289VPS27YNR006WverifiedEndosomal protein that forms a complex with Hse1p; required forrecycling Golgi proteins, forming lumenalmembranes and sorting ubiquitinated proteinsdestined for degradation; has UbiquitinInteraction Motifs which bind ubiquitin (Ubi4p) 0.026050.39108&radic S000002171HEX3YDL013WverifiedProtein containing a RING finger domain that interacts withSlx8p; mutant phenotypes and geneticinteractions suggest a role in sumoylation andin genome stability 0.025680.38911 S000001243RPN10YHR200WverifiedNon-ATPase base subunit of the 19S regulatory particle (RP) ofthe 26S proteasome; N-terminus plays a role inmaintaining the structural integrity of the RP;binds selectively to polyubiquitin chains;homolog of the mammalian S5a protein 0.025270.38621&radic&radic S000005174ELA1YNL230CverifiedElongin A, F-box protein that forms a heterodimer with Elc1pand participates in transcription elongation 0.024520.37639&radic&radic&radic&radic&radic S000002731PEP7YDR323CverifiedMultivalent adaptor protein that facilitates vesicle-mediatedvacuolar protein sorting by ensuringhigh-fidelity vesicle docking and fusion, whichare essential for targeting of vesicles to theendosome; required for vacuole inheritance 0.023790.37374&radic&radic&radic&radic S000003099SNT2YGL131CverifiedDNA binding protein with similarity to the S. pombe Snt2protein 0.023690.37027&radic S000001335SPO22YIL073CverifiedMeiosis-specific protein essential for chromosome synapsis,similar to phospholipase A2, involved incompletion of nuclear divisions during meiosis;induced early in meiosis 0.02260.36439&radic&radic&radic&radic&radic S000001292SSM4YIL030CverifiedUbiquitin-protein ligase of the ER/nuclear envelope, requiredfor degradation of Alpha2p and other proteinscontaining a Deg1 degradation signal; ssm4mutation suppresses mRNA instability caused byan rna14 mutation &radic0.02220.36111&radic&radic&radic S000000316CYC8YBR112CverifiedGeneral transcriptional co-repressor, acts together with Tup1p;also acts as part of a transcriptionalco-activator complex that recruits the SWI/SNFand SAGA complexes to promoters 0.021620.3569&radic S000006128TYW1YPL207WuncharacterizedHypothetical protein 0.020480.34643&radic S000004376IKI3YLR384CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;maintains structural integrity of Elongator;homolog of human IKAP, mutations in which causefamilial dysautonomia (FD) 0.020680.34643&radic&radic S000001159TOM71YHR117WverifiedMitochondrial outer membrane protein with similarity to Tom70p;probable minor component of the TOM(translocase of outer membrane) complexresponsible for recognition and import ofmitochondrially directed proteins 0.020510.34643&radic&radic S000002652PEX5YDR244WverifiedPeroxisomal membrane signal receptor for C-terminal tripeptidesignal sequence (PTS1) of peroxisomal matrixproteins, required for peroxisomal matrixprotein import, tetratricopeptide repeatprotein, also involved in PTS1-independentimport 0.019740.34096&radic&radic S000004197HRD3YLR207WverifiedResident protein of the ER membrane that plays a central rolein ER-associated protein degradation (ERAD),forms HRD complex with Hrd1p and ERADdeterminants that engages in lumen to cytosolcommunication and coordination of ERAD events &radic0.019590.34015&radic&radic&radic&radic S000000994HSE1YHL002WverifiedSubunit of the endosomal Vps27p-Hse1p complex required forsorting of ubiquitinated membrane proteins intointralumenal vesicles prior to vacuolardegradation, as well as for recycling of Golgiproteins and formation of lumenal membranes 0.019490.33852&radic S000004442HMG2YLR450WverifiedOne of two isozymes of HMG-CoA reductase that convert HMG-CoAto mevalonate, a rate-limiting step in sterolbiosynthesis; overproduction induces assemblyof peripheral ER membrane arrays and shortnuclear-associated membrane stacks 0.019370.33745&radic&radic S000002991PIB2YGL023CverifiedProtein binding phosphatidylinositol 3-phosphate, involved intelomere-proximal repression of geneexpression; similar to Fab1 and Vps27 0.019060.3342&radic S000003778ESS1YJR017CverifiedPeptidylprolyl-cis/trans-isomerase (PPIase) specific forphosphorylated serine and threonine residuesN-terminal to proline; regulatesphosphorylation of the RNA polymerase II largesubunit (Rpo21p) C-terminal domain 0.018950.3342&radic&radic S000003355PPT1YGR123CverifiedProtein serine/threonine phosphatase with similarity to humanphosphatase PP5; present in both the nucleusand cytoplasm; expressed during logarithmicgrowth 0.018570.32984&radic&radic S000001915FAB1YFR019Wverified1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membranekinase that generates phosphatidylinositol(3,5)P2, which is involved in vacuolar sortingand homeostasis 0.018470.32984 S000002728SWA2YDR320CverifiedAuxilin-like protein involved in vesicular transport;clathrin-binding protein required for uncoatingof clathrin-coated vesicles 0.017840.32346&radic&radic&radic S000002743MSN5YDR335WverifiedKaryopherin involved in nuclear import and export; shown to beresponsible for nuclear import of replicationprotein A and for export of several proteinsincluding Swi6p, Far1p, and Pho4p; cargodissociation involves binding to RanGTP 0.017140.31766&radic S000000146SEC17YBL050WverifiedPeripheral membrane protein required for vesicular transportbetween ER and Golgi and for the 'priming' stepin homotypic vacuole fusion, part of thecis-SNARE complex; has similarity to alpha-SNAP 0.017250.31766&radic S000005505CTR9YOL145CverifiedComponent of the Paf1p complex, which is a large complex thatbinds to and modulates the activity of RNApolymerase II and is required for expression ofa subset of genes, including cyclin genes;contains TPR repeats 0.016840.31627&radic&radic S000002506BMH2YDR099Wverified14-3-3 protein, minor isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.016460.31271&radic&radic&radic&radic&radic S000003105SEC27YGL137WverifiedEssential beta'-coat protein of the COPI coatomer, involved inER-to-Golgi and Golgi-to-ER transport; containsWD40 domains that mediate cargo selectiveinteractions; 45% sequence identity tomammalian beta'-COP 0.016430.31161&radic S000003848YJR088CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum 0.015610.30226&radic S000004540HMG1YML075CverifiedOne of two isozymes of HMG-CoA reductase that catalyzes theconversion of HMG-CoA to mevalonate, which is arate-limiting step in sterol biosynthesis;localizes to the nuclear envelope;overproduction induces the formation ofkarmellae 0.015240.29918 S000003945HIF1YLL022CverifiedNon-essential component of the HAT-B histone acetyltransferasecomplex (Hat1p-Hat2p-Hif1p), localized to thenucleus; has a role in telomeric silencing 0.01510.29715&radic S000006312RPN7YPR108WverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome,similar to another S. cerevisiae regulatorysubunit, Rpn5p, as well as to mammalianproteasome subunits 0.014840.29539&radic&radic&radic S000004434SIR3YLR442CverifiedSilencing protein that interacts with Sir2p and Sir4p, andhistone H3 and H4 tails, to establish atranscriptionally silent chromatin state;required for spreading of silenced chromatin;recruited to chromatin through interaction withRap1p 0.014540.28925 S000001377NUP159YIL115CverifiedSubunit of the nuclear pore complex that is found exclusivelyon the cytoplasmic side, forms a subcomplexwith Nup82p and Nsp1p, required for mRNA export 0.014530.28925&radic S000005133SRP1YNL189WverifiedKaryopherin alpha homolog, forms a dimer with karyopherin betaKap95p to mediate import of nuclear proteins,binds the nuclear localization signal of thesubstrate during import; may also play a rolein regulation of protein degradation 0.014480.28925&radic S000004196ENT2YLR206WverifiedEpsin-like protein required for endocytosis and actin patchassembly and functionally redundant with Ent1p;contains clathrin-binding motif at C-terminus 0.014340.28925&radic&radic S000004850BCH1YMR237WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p; may interact with ribosomes 0.014110.28682&radic S000002635SIR4YDR227WverifiedSilent information regulator that, together with SIR2 and SIR3,is involved in assembly of silent chromatindomains at telomeres and the silent mating-typeloci; potentially phosphorylated by Cdc28p;some alleles of SIR4 prolong lifespan 0.013930.28429&radic&radic&radic S000000763RAD23YEL037CverifiedProtein with ubiquitin-like N terminus, recognizes and bindsdamaged DNA (with Rad4p) during nucleotideexcision repair; regulates Rad4p levels,subunit of Nuclear Excision Repair Factor 2(NEF2); homolog of human HR23A and HR23Bproteins 0.013860.28407&radic&radic S000000656TAH1YCR060WverifiedHSP90 cofactor; interacts with Hsp82p, Pih1p, Rvb1 and Rvb2,contains a single TPR domain with at least twoTPR motifs 0.013570.27697&radic S000002583NGG1YDR176WverifiedTranscriptional regulator involved in glucose repression ofGal4p-regulated genes; component oftranscriptional adaptor and histoneacetyltransferase complexes, the ADA complex,the SAGA complex, and the SLIK complex 0.013380.27697&radic&radic&radic&radic S000002483RAD55YDR076WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad57p 0.013320.27697&radic&radic&radic&radic&radic S000006356URN1YPR152CuncharacterizedPre-mRNA splicing factor associated with the U2-U5-U6 snRNPs,the RES complex, and the Prp19-associatedcomplex (NTC); null mutation displays syntheticgenetic interactions with mutations affectingU2 snRNA and pre-mRNA splicing factors 0.01320.27549&radic&radic&radic S000004107CLF1YLR117CverifiedEssential splicesome assembly factor; contains multipletetratricopeptide repeat (TPR) protein-bindingmotifs and interacts specifically with manyspliceosome components, may serve as a scaffoldduring splicesome assembly 0.012950.27334&radic&radic S000004283SEC72YLR292CverifiedNon-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66pand Sec72p); with Sec61 complex, Kar2p/BiP andLhs1p forms a channel competent forSRP-dependent and post-translationalSRP-independent protein targeting and importinto the ER 0.012890.27231&radic&radic&radic S000002320ENT1YDL161WverifiedEpsin-like protein involved in endocytosis and actin patchassembly and functionally redundant with Ent2p;binds clathrin via a clathrin-binding domainmotif at C-terminus 0.012890.27231 S000001790NUP133YKR082WverifiedSubunit of the Nup84p subcomplex of the nuclear pore complex(NPC), localizes to both sides of the NPC,required to establish a normalnucleocytoplasmic concentration gradient of theGTPase Gsp1p 0.01290.27231&radic S000001803MLP1YKR095WverifiedMyosin-like protein associated with the nuclear envelope,connects the nuclear pore complex with thenuclear interior; involved with Tel1p intelomere length control; involved with Pml1pand Pml39p in nuclear retention of unsplicedmRNAs 0.012680.26994 S000005065TOM70YNL121CverifiedComponent of the TOM (translocase of outer membrane) complexresponsible for recognition and initial importsteps for all mitochondrially directedproteins; acts as a receptor for incomingprecursor proteins 0.012690.26994&radic S000004832ESC1YMR219WverifiedProtein localized to the nuclear periphery, involved intelomeric silencing; interacts with PAD4-domainof Sir4p 0.012560.26917&radic&radic&radic&radic S000000063NUP60YAR002WverifiedSubunit of the nuclear pore complex (NPC), functions to anchorNup2p to the NPC in a process controlled by thenucleoplasmic concentration of Gsp1p-GTP;potential Cdc28p substrate; involved intelomere maintenance 0.012250.26492&radic&radic S000003827TOR1YJR066WverifiedPIK-related protein kinase and rapamycin target; subunit ofTORC1, a complex that controls growth inresponse to nutrients by regulatingtranslation, transcription, ribosomebiogenesis, nutrient transport and autophagy;involved in meiosis 0.012320.26492&radic&radic&radic S000004497SRC1YML034WverifiedProtein with a putative role in sister chromatid segregation,potentially phosphorylated by Cdc28p; greenfluorescent protein (GFP)-fusion proteinlocalizes to the nuclear periphery 0.012240.26492&radic&radic S000001141TRA1YHR099WverifiedSubunit of SAGA and NuA4 histone acetyltransferase complexes;interacts with acidic activators (e.g., Gal4p)which leads to transcription activation;similar to human TRRAP, which is a cofactor forc-Myc mediated oncogenic transformation 0.012320.26492 S000002566SAC3YDR159WverifiedNuclear pore-associated protein, forms a complex with Thp1pthat is involved in transcription and in mRNAexport from the nucleus 0.012180.26458&radic&radic S000004798HSC82YMR186WverifiedCytoplasmic chaperone of the Hsp90 family, redundant infunction and nearly identical with Hsp82p, andtogether they are essential; expressedconstitutively at 10-fold higher basal levelsthat HSP82 and induced 2-3 fold by heat shock 0.011950.26066&radic&radic&radic&radic&radic S000004575PML39YML107CverifiedProtein required for nuclear retention of unspliced pre-mRNAsalong with Mlp1p and Pml1p; anchored to nuclearpore complex via Mlp1p and Mlp2p; found withthe subset of nuclear pores farthest from thenucleolus; may interact with ribosomes 0.011670.25771&radic S000000399MSI1YBR195CverifiedSubunit of chromatin assembly factor I (CAF-I), regulates theRAS/cAMP pathway via sequestration of Npr1pkinase; localizes to the nucleus and cytoplasm;homologous to human retinoblastoma bindingproteins RbAp48 and RbAp46 0.011550.25615&radic&radic&radic S000000340MEC1YBR136WverifiedGenome integrity checkpoint protein and PI kinase superfamilymember; signal transducer required for cellcycle arrest and transcriptional responsesprompted by damaged or unreplicated DNA;monitors and participates in meioticrecombination 0.011210.25117 S000006345KAR3YPR141CverifiedMinus-end-directed microtubule motor that functions in mitosisand meiosis, localizes to the spindle pole bodyand localization is dependent on functionalCik1p, required for nuclear fusion duringmating; potential Cdc28p substrate 0.010850.24634&radic&radic&radic S000001877GNA1YFL017CverifiedEvolutionarily conserved glucosamine-6-phosphateacetyltransferase required for multiple cellcycle events including passage through START,DNA synthesis, and mitosis; involved inUDP-N-acetylglucosamine synthesis, formsGlcNAc6P from AcCoA 0.010540.24139&radic&radic&radic&radic S000002872SPP41YDR464WverifiedProtein involved in negative regulation of expression ofspliceosome components PRP4 and PRP3 0.010260.23807&radic&radic S000002461CDC34YDR054CverifiedUbiquitin-conjugating enzyme or E2; together with Skp1p, Rbx1p,Cdc53p, and an F-box protein, forms aubiquitin-protein ligase called the SCF complexwhich regulates cell cycle progression bytargeting key substrates for degradation 0.010140.23472&radic&radic&radic S000002265MSS2YDL107WverifiedPeripherally bound inner membrane protein of the mitochondrialmatrix, required for export of C-terminal tailof Cox2p through the inner membrane 0.010130.23472&radic S000005825BUD7YOR299WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p, from the Golgi to plasma membrane 0.010140.23472&radic&radic S000006373JIP5YPR169WuncharacterizedNucleolar protein of unknown function, exhibits a physicalinteraction with Bre1p 0.010.23407&radic&radic&radic&radic&radic S000003675YUR1YJL139CverifiedMannosyltransferase of the KTR1 family, involved in proteinN-glycosylation; located in the Golgi apparatus 0.009920.23286 S000004571NUP188YML103CverifiedSubunit of the nuclear pore complex (NPC), involved in thestructural organization of the complex and ofthe nuclear envelope, also involved in nuclearenvelope permeability, interacts with Pom152pand Nic96p 0.009850.23051 S000003136HUR1YGL168WuncharacterizedProtein required for hydroxyurea resistance; has possible rolesin DNA replication and maintenance of propertelomere length 0.009840.23051&radic S000005775APC5YOR249CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.009790.23051&radic&radic&radic&radic&radic S000003216BRR6YGL247WverifiedEssential nuclear envelope integral membrane protein requiredfor nuclear transport; depletion altersnucleoporin distribution and nuclear envelopemorphology, suggesting a role in the spatialorganization of nuclear pores 0.00980.23051&radic S000003430YPP1YGR198WverifiedCargo-transport protein involved in endocytosis; may interactwith ribosomes, based on co-purificationexperiments; GFP-fusion protein localizes tothe cytoplasm; YGR198W is an essential gene 0.009760.22804&radic S000001884WWM1YFL010CverifiedWW domain containing protein of unknown function; binds toMca1p, a caspase-related protease thatregulates H2O2-induced apoptosis;overexpression causes Gi phase growth arrestand clonal death that is suppressed byoverexpression of MCA1 0.009640.2279&radic&radic S000002831CAD1YDR423CverifiedAP-1-like bZIP transcriptional activator involved in multiplestress responses, iron metabolism, andpleiotropic drug resistance; controls a set ofgenes involved in stabilizing proteins, bindsconsensus sequence TTACTAA; 5' UTR containsuORFs 0.009520.22571&radic&radic&radic&radic&radic S000001175NSG1YHR133CverifiedProtein involved in regulation of sterol biosynthesis;specifically stabilizes Hmg2p, one of twoHMG-CoA isoenzymes that catalyze therate-limiting step in sterol biosynthesis;homolog of mammalian INSIG proteins 0.009540.22571&radic&radic S000001338SEC28YIL076WverifiedEpsilon-COP subunit of the coatomer; regulates retrogradeGolgi-to-ER protein traffic; stabilizes Cop1p,the alpha-COP and the coatomer complex;non-essential for cell growth 0.009510.22571&radic S000004200CLB4YLR210WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the G2/M transition; may beinvolved in DNA replication and spindleassembly; accumulates during S phase and G2,then targeted for ubiquitin-mediateddegradation 0.009440.22372&radic&radic&radic&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p 0.009350.22287&radic&radic S000000359CNS1YBR155WverifiedTPR-containing co-chaperone; binds both Hsp82p (Hsp90) andSsa1p (Hsp70) and stimulates the ATPaseactivity of SSA1, ts mutants reduce Hsp82pfunction while over expression suppresses thephenotypes of an HSP82 ts allele and a cpr7deletion 0.009320.22287&radic S000000886YER084WDubiousHypothetical protein 0.009310.2228&radic&radic&radic&radic S000003597NUP82YJL061WverifiedSubunit of the nuclear pore complex (NPC), forms a subcomplexwith Nup159p and Nsp1p, interacts with Nup116pand is required for proper localization ofNup116p in the NPC 0.009230.22059&radic S000002568YDR161WUncharacterizedinteracts with PP2C 0.009260.22059&radic&radic&radic S000003803NUP85YJR042WverifiedSubunit of the Nup84p subcomplex of the nuclear pore complex(NPC), required for assembly of the subcomplexand also for formation of the nucleocytoplasmicGsp1p concentration gradient that plays a rolein nuclear trafficking 0.009230.22059 S000002824SYF1YDR416WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression; similar to Drosophila crooked neckprotein 0.00920.22059&radic&radic&radic&radic&radic S000002494RRP1YDR087CverifiedEssential evolutionarily conserved nucleolar protein necessaryfor biogenesis of 60S ribosomal subunits andprocessing of pre-rRNAs to mature rRNAs,associated with several distinct 66Spre-ribosomal particles 0.009250.22059&radic&radic&radic&radic&radic&radic S000002200SIR2YDL042CverifiedConserved NAD+ dependent histone deacetylase of the Sirtuinfamily involved in regulation of lifespan;plays roles in silencing at HML, HMR,telomeres, and the rDNA locus; negativelyregulates initiation of DNA replication 0.009220.22059 S000001052RPL27AYHR010WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl27Bp and has similarity to ratL27 ribosomal protein 0.009250.22059&radic&radic S000001012OPI1YHL020CverifiedTranscriptional regulator of a variety of genes;phosphorylation by protein kinase A stimulatesOpi1p function in negative regulation ofphospholipid biosynthetic genes; involved intelomere maintenance 0.009250.22059&radic&radic S000002235VAM6YDL077CverifiedVacuolar protein that plays a critical role in the tetheringsteps of vacuolar membrane fusion byfacilitating guanine nucleotide exchange onsmall guanosine triphosphatase Ypt7p 0.009080.21791&radic S000002274NUP84YDL116WverifiedSubunit of the nuclear pore complex (NPC), forms a subcomplexwith Nup85p, Nup120p, Nup145p-C, Sec13p, andSeh1p that plays a role in nuclear mRNA exportand NPC biogenesis 0.008970.21633 S000001551NUP100YKL068WverifiedSubunit of the nuclear pore complex (NPC) that is localized toboth sides of the pore; contains a repetitiveGLFG motif that interacts with mRNA exportfactor Mex67p and with karyopherin Kap95p;homologous to Nup116p 0.008980.21633&radic&radic&radic S000004008POM34YLR018CverifiedIntegral membrane protein of the nuclear pore; has an importantrole in maintaining the architecture of thepore complex 0.009020.21633&radic S000002247YDL089WUncharacterizedProtein of unknown function; interacts with meiotic division protein Csm1p; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery, potential Cdc28p substrate 0.008940.21589 S000003281SCM4YGR049WverifiedPotential regulatory effector of CDC4 function, suppresses atemperature-sensitive allele of CDC4,tripartite protein structure in which a chargedregion separates two uncharged domains, notessential for mitosis or meiosis 0.008890.21434&radic&radic&radic&radic S000006376YPR172WUncharacterizedProtein of unknown function, transcriptionally activated by Yrm1p along with genes involved in multidrug resistance 0.008880.21434&radic&radic&radic&radic&radic S000005941ULP1YPL020CverifiedUbl (ubiquitin-like protein)-specific protease that cleavesSmt3p protein conjugates; specifically requiredfor cell cycle progression; associates withnucleoporins and may interact with septin ringsduring telophase 0.00890.21434 S000005624NUP1YOR098CverifiedNuclear pore complex (NPC) subunit, involved in proteinimport/export and in export of RNAs, possiblekaryopherin release factor that acceleratesrelease of karyopherin-cargo complexes aftertransport across NPC; potential Cdc28psubstrate 0.008850.21371&radic&radic S000005572DBP5YOR046CverifiedCytoplasmic ATP-dependent RNA helicase of the DEAD-box familyinvolved in mRNA export from the nucleus 0.008840.21371&radic&radic S000003793CPR7YJR032WverifiedPeptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzesthe cis-trans isomerization of peptide bondsN-terminal to proline residues; binds to Hsp82pand contributes to chaperone activity 0.008690.21024 S000004762NUP53YMR153WverifiedSubunit of the nuclear pore complex (NPC), interacts withkaryopherin Kap121p or with Nup170p viaoverlapping regions of Nup53p, involved inactivation of the spindle checkpoint mediatedby the Mad1p-Mad2p complex 0.008770.21024&radic&radic S000001726YKR018CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.008680.21024&radic S000004327NUP2YLR335WverifiedProtein involved in nucleocytoplasmic transport, binds toeither the nucleoplasmic or cytoplasmic facesof the nuclear pore complex depending onRan-GTP levels; also has a role in chromatinorganization 0.008670.21024&radic S000000682CSM1YCR086WverifiedNucleolar protein that forms a complex with Lrs4p which bindsMam1p at kinetochores during meiosis I tomediate accurate chromosome segregation, may beinvolved in premeiotic DNA replication;possible role in telomere maintenance 0.008640.20976&radic&radic S000002246ASM4YDL088CverifiedNuclear pore complex subunit, part of a subcomplex alsocontaining Nup53p, Nup170p, and Pse1p 0.008620.20969 S000002866HEH2YDR458CuncharacterizedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the nuclearperiphery 0.008620.20969 S000003306SMD1YGR074WverifiedCore Sm protein Sm D1; part of heteroheptameric complex (withSmb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D1 0.008560.20873&radic&radic&radic&radic&radic S000003957RIX7YLL034CverifiedPutative ATPase of the AAA family, required for export ofpre-ribosomal large subunits from the nucleus;distributed between the nucleolus, nucleoplasm,and nuclear periphery depending on growthconditions 0.008560.20873&radic&radic&radic S000000259PRP6YBR055CverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.008520.20814&radic S000000175NUP170YBL079WverifiedAbundant subunit of the nuclear pore complex (NPC), requiredfor proper localization of specificnucleoporins within the NPC, involved innuclear envelope permeability and in chromosomesegregation, has similarity to Nup157p 0.008510.20686&radic&radic S000004339KAP95YLR347CverifiedKaryopherin beta, forms a dimeric complex with Srp1p (Kap60p)that mediates nuclear import of cargo proteinsvia a nuclear localization signal (NLS),interacts with nucleoporins to guide transportacross the nuclear pore complex 0.008480.20686 S000003434PCT1YGR202CverifiedCholinephosphate cytidylyltransferase, also known asCTP:phosphocholine cytidylyltransferase,rate-determining enzyme of the CDP-cholinepathway for phosphatidylcholine synthesis,inhibited by Sec14p, activated uponlipid-binding 0.008360.20493&radic&radic S000004924GAS1YMR307WverifiedBeta-1.3-glucanosyltransferase, required for cell wallassembly; localizes to the cell surface via aglycosylphosphatidylinositol (GPI) anchor 0.008340.20493&radic S000004760YIM1YMR152WverifiedProtein of unknown function; null mutant displays sensitivityto DNA damaging agents; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 0.008290.20449&radic&radic&radic S000001669MTR2YKL186CverifiedmRNA transport regulator, essential nuclear protein; Mex67p andMtr2p form a mRNA export complex which binds toRNA 0.008260.20284&radic S000004279MEC3YLR288CverifiedDNA damage and meiotic pachytene checkpoint protein; subunit ofa heterotrimeric complex (Rad17p-Mec3p-Ddc1p)that forms a sliding clamp, loaded onto partialduplex DNA by a clamp loader complex; homologof human and S. pombe Hus1 0.007990.19915&radic&radic&radic&radic&radic&radic S000000743GTT3YEL017WuncharacterizedProtein of unknown function with a possible role in glutathionemetabolism, as suggested by computationalanalysis of large-scale protein-proteininteraction data; GFP-fusion protein localizesto the nuclear periphery 0.008030.19915&radic&radic S000003576NUP192YJL039CverifiedEssential structural subunit of the nuclear pore complex (NPC),localizes to the nuclear periphery of nuclearpores, homologous to human p205 0.007980.19915 S000001502RAM2YKL019WverifiedAlpha subunit of both the farnesyltransferase and type Igeranylgeranyltransferase that catalyzeprenylation of proteins containing a CAAXconsensus motif; essential protein required formembrane localization of Ras proteins anda-factor 0.008040.19915&radic&radic S000006323CLB2YPR119WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 0.008040.19915&radic&radic&radic S000006131SRP72YPL210CverifiedCore component of the signal recognition particle (SRP)ribonucleoprotein (RNP) complex that functionsin targeting nascent secretory proteins to theendoplasmic reticulum (ER) membrane 0.008020.19915&radic&radic S000004559GIM5YML094WverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 0.007940.198&radic S000002668SWM1YDR260CverifiedSubunit of the anaphase-promoting complex, which is an E3ubiquitin ligase that regulates themetaphase-anaphase transition and exit frommitosis; required for activation of thedaughter-specific gene expression and sporewall maturation 0.007920.198&radic&radic S000006090MEX67YPL169CverifiedPoly(A)RNA binding protein involved in nuclear mRNA export,component of the nuclear pore; ortholog ofhuman TAP 0.007920.198&radic&radic S000003437YGR205WVerifiedATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases 0.00790.1972&radic&radic S000003635CHS6YJL099WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p, from the Golgi to plasma membrane 0.007830.19683 S000005055CYB5YNL111CverifiedCytochrome b5, involved in the sterol and lipid biosynthesispathways; required for sterol C5-6 and fattyacid desaturation 0.007820.19606&radic S000003859YJR098CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; detectable in highly purified mitochondria 0.007820.19606&radic S000002971CDH1YGL003CverifiedCell-cycle regulated activator of the anaphase-promotingcomplex/cyclosome (APC/C), which directsubiquitination of mitotic cyclins resulting inexit from mitosis; targets the APC/C tospecific substrates including CDC20, ASE1 andCIN8 0.007730.19466&radic&radic S000000907NUP157YER105CverifiedAbundant subunit of the nuclear pore complex (NPC), present onboth sides of the NPC, has similarity toNup170p 0.007710.19459 S000000909GLE2YER107CverifiedComponent of the nuclear pore complex required forpolyadenylated RNA export but not for proteinimport, homologous to S. pombe Rae1p 0.007620.19301 S000003351NUP57YGR119CverifiedEssential subunit of the nuclear pore complex (NPC), functionsas the organizing center of an NPC subcomplexcontaining Nsp1p, Nup49p, Nup57p, and Nic96p 0.007580.19151&radic&radic S000003577NSP1YJL041WverifiedEssential component of the nuclear pore complex, which mediatesnuclear import and export 0.00750.19052&radic S000005559EXO1YOR033Cverified5'-3' exonuclease and flap-endonuclease involved inrecombination, double-strand break repair andDNA mismatch repair; member of the Rad2pnuclease family, with conserved N and Inuclease domains 0.007420.18924&radic&radic&radic&radic&radic S000001069RPN1YHR027CverifiedNon-ATPase base subunit of the 19S regulatory particle of the26S proteasome; may participate in therecognition of several ligands of theproteasome; contains a leucine-rich repeat(LRR) domain, a site for protein?proteininteractions 0.007420.18924&radic&radic&radic&radic S000003584UBX6YJL048CuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p, transcription isrepressed when cells are grown in mediacontaining inositol and choline 0.007390.18924 S000003188YGL220WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus 0.007360.18791&radic&radic&radic&radic S000004422SEN1YLR430WverifiedPutative helicase required for RNA polymerase II transcriptiontermination and processing of RNAs; homolog ofSenataxin which causes Ataxia-OculomotorApraxia 2 and a dominant form of amyotrophiclateral sclerosis 0.007290.18734&radic S000001495PRP40YKL012WverifiedU1 snRNP protein involved in splicing, interacts with thebranchpoint-binding protein during theformation of the second commitment complex 0.007230.18582&radic&radic&radic S000006177CLN2YPL256CverifiedG1 cyclin involved in regulation of the cell cycle; activatesCdc28p kinase to promote the G1 to S phasetransition; late G1 specific expression dependson transcription factor complexes, MBF(Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) 0.007270.18582&radic&radic&radic S000004092APC9YLR102CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.007220.18582&radic&radic&radic&radic&radic S000001504MAK11YKL021CverifiedProtein essential for cell growth and replication of M dsRNAvirus; contains four beta-transducin repeats 0.007280.18582&radic&radic&radic&radic&radic&radic S000003618IML2YJL082WverifiedProtein of unknown function; the authentic, non-tagged proteinis detected in highly purified mitochondria inhigh-throughput studies 0.007230.18582&radic S000006259SEC8YPR055WverifiedEssential 121kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 0.007260.18582&radic&radic S000005475PAP2YOL115WverifiedCatalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), anuclear poly (A) polymerase complex involved inRNA quality control; catalyzes polyadenylationof unmodified tRNAs, and snoRNA and rRNAprecursors; disputed role as a DNA polymerase 0.00720.18524&radic&radic&radic S000004368PSY3YLR376CuncharacterizedProtein of unknown function; deletion results in a mutatorphenotype suggesting a role for this protein asa mutational suppressor; deletion increasessensitivity to anticancer drugs oxaliplatin andcisplatin but not mitomycin C 0.007210.18524&radic&radic S000001688LOS1YKL205WverifiedNuclear pore protein involved in nuclear export of pre-tRNA 0.007180.18431&radic S000004721FMP24YMR115WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.007170.18431&radic S000003054MAD1YGL086WverifiedCoiled-coil protein involved in the spindle-assemblycheckpoint; phosphorylated by Mps1p uponcheckpoint activation which leads to inhibitionof the activity of the anaphase promotingcomplex; forms a complex with Mad2p 0.007170.18431&radic S000001686TOR2YKL203CverifiedPIK-related protein kinase and rapamycin target; subunit ofTORC1, a complex that regulates growth inresponse to nutrients and TORC2, a complex thatregulates cell-cycle dependent polarization ofthe actin cytoskeleton; involved in meiosis 0.007110.18319&radic S000002527TRM1YDR120CverifiedtRNA methyltransferase, localizes to both the nucleus andmitochondrion to produce the modified baseN2,N2-dimethylguanosine in tRNAs in bothcompartments 0.007050.18253&radic&radic&radic S000004493NDC1YML031WverifiedNuclear envelope protein with multiple putative transmembranedomains, required for nuclear pore complexassembly and spindle pole body duplication;required for meiosis II 0.007030.18219&radic&radic S000002140YAL064C-AUncharacterizedHypothetical protein 0.007030.18177&radic S000002777XRS2YDR369CverifiedProtein required for DNA repair; component of the Mre11complex, which is involved in double strandbreaks, meiotic recombination, telomeremaintenance, and checkpoint signaling 0.007020.18177&radic&radic&radic S000005102PGA1YNL158WverifiedEssential protein required for maturation of Gas1p and Pho8p;green fluorescent protein (GFP)-fusion proteinlocalizes to the nuclear periphery; hassynthetic genetic interations with secretorypathway genes 0.007020.18177 S000003223FZF1YGL254WverifiedTranscription factor involved in sulfite metabolism, soleidentified regulatory target is SSU1,overexpression suppresses sulfite-sensitivityof many unrelated mutants due tohyperactivation of SSU1, contains five zincfingers 0.007020.18177&radic&radic&radic&radic S000001167YHR125WDubiousHypothetical protein 0.0070.18016&radic&radic&radic&radic S000000440ABD1YBR236CverifiedMethyltransferase, catalyzes the transfer of a methyl groupfrom S-adenosylmethionine to the GpppN terminusof capped mRNA 0.006910.17959&radic&radic S000002736SKP1YDR328CverifiedEvolutionarily conserved kinetochore protein that is part ofmultiple protein complexes, including the SCFubiquitin ligase complex, the CBF3 complex thatbinds centromeric DNA, and the RAVE complexthat regulates assembly of the V-ATPase &radic0.006890.17949&radic&radic&radic S000002255RPN6YDL097CverifiedEssential, non-ATPase regulatory subunit of the 26S proteasomelid required for the assembly and activity ofthe 26S proteasome; the human homolog (S9protein) partially rescues Rpn6p depletion 0.006890.17949&radic S000001112TRM5YHR070WverifiedtRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacentto the anticodon that has a role in preventionof frameshifting; highly conserved acrossArchaea, Bacteria, and Eukarya 0.006850.17829&radic&radic&radic&radic S000001049ERG11YHR007CverifiedLanosterol 14-alpha-demethylase, catalyzes the C-14demethylation of lanosterol to form4,4''-dimethylcholesta-8,14,24-triene-3-beta-ol in theergosterol biosynthesis pathway; member of thecytochrome P450 family 0.006810.17791&radic S000001051YHR009CUncharacterizedPutative protein of unknown function; not an essential gene 0.006780.17666&radic&radic&radic S000002976PMA1YGL008CverifiedPlasma membrane H+-ATPase, pumps protons out of the cell; majorregulator of cytoplasmic pH and plasma membranepotential; part of the P2 subgroup ofcation-transporting ATPases 0.006730.17605&radic&radic&radic S000002525APC4YDR118WverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.006710.17578&radic&radic&radic&radic&radic S000005436MDM20YOL076WverifiedNon-catalytic subunit of the NatB N-terminal acetyltransferase,which catalyzes acetylation of theamino-terminal methionine residues of allproteins beginning with Met-Asp or Met-Glu andof some proteins beginning with Met-Asn orMet-Met; involved in mitochondrial inheritanceand actin assembly 0.006710.17578&radic&radic S000003055MMS2YGL087CverifiedProtein involved in error-free postreplication DNA repair;forms a heteromeric complex with Ubc13p thathas a ubiquitin-conjugating activity;cooperates with chromatin-associated RINGfinger proteins, Rad18p and Rad5p 0.006680.1752&radic&radic&radic S000002256SNU23YDL098CverifiedComponent of U4/U6.U5 snRNP involved in mRNA splicing viaspliceosome 0.00660.17302&radic S000001898NIC96YFR002WverifiedComponent of the nuclear pore complex, required for nuclearpore formation; forms a subcomplex with Nsp1p,Nup57p, and Nup49p 0.006670.17302 S000003060NUP145YGL092WverifiedEssential nucleoporin, catalyzes its own cleavage in vivo togenerate a C-terminal fragment that assemblesinto the Nup84p subcomplex of the nuclear porecomplex, and an N-terminal fragment of unknownfunction that is homologous to Nup100p 0.006650.17302&radic S000000792HPA3YEL066WverifiedD-Amino acid N-acetyltransferase, catalyzes N-acetylation ofD-amino acids through ordered bi-bi mechanismin which acetyl-CoA is first substrate boundand CoA is last product liberated; similar toHpa2p, acetylates histones weakly in vitro 0.006540.1724&radic&radic S000004214YLR224WUncharacterizedF-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene 0.006510.17149&radic&radic S000005551HST3YOR025WverifiedMember of the Sir2 family of NAD(+)-dependent proteindeacetylases; involved along with Hst4p intelomeric silencing, cell cycle progression,radiation resistance, genomic stability andshort-chain fatty acid metabolism 0.006530.17149&radic&radic&radic S000004700CTF13YMR094WverifiedSubunit of the CBF3 complex, which binds to the CDE III elementof centromeres, bending the DNA upon binding,and may be involved in sister chromatidcohesion during mitosis 0.006520.17149&radic S000000065RFA1YAR007CverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 0.006520.17149&radic&radic&radic S000001464MND2YIR025WverifiedSubunit of the anaphase-promoting complex (APC); needed formeiotic nuclear division 0.006430.1702&radic&radic&radic S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.006430.17009&radic&radic&radic&radic&radic S000001932CDC26YFR036WverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.006430.17009&radic&radic S000002493SSS1YDR086CverifiedSubunit of the Sec61p translocation complex(Sec61p-Sss1p-Sbh1p) that forms a channel forpassage of secretory proteins through theendoplasmic reticulum membrane, and of theSsh1p complex (Ssh1p-Sbh2p-Sss1p); interactswith Ost4p and Wbp1p 0.00640.16913&radic&radic&radic S000002603REF2YDR195WverifiedRNA-binding protein involved in the cleavage step of mRNA3'-end formation prior to polyadenylation, andin snoRNA maturation; part of holo-CPFsubcomplex APT, which associates with 3'-endsof snoRNA- and mRNA-encoding genes 0.006390.16913&radic&radic&radic S000001249SKN7YHR206WverifiedNuclear response regulator and transcription factor, part of abranched two-component signaling system;required for optimal induction of heat-shockgenes in response to oxidative stress; involvedin osmoregulation 0.006340.16823&radic&radic&radic S000004736POM152YMR129WverifiedNuclear pore membrane glycoprotein; may be involved induplication of nuclear pores and nuclear porecomplexes during S-phase; 0.006270.1666 S000005544ROD1YOR018WverifiedMembrane protein; overexpression confers resistance to the GSTsubstrate o-dinitrobenzene as well as to zincand calcium; contains 2 PY motifs, which arerequired for Rod1p interaction with Rsp5p, ahect-type ubiquitin ligase 0.006280.1666&radic&radic S000002764SPC110YDR356WverifiedInner plaque spindle pole body (SPB) component, ortholog ofhuman kendrin; involved in connecting nuclearmicrotubules to SPB; interacts withTub4p-complex and calmodulin; phosphorylated byMps1p in cell cycle-dependent manner 0.006260.1666&radic&radic&radic&radic S000002198NAT1YDL040CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.006290.1666&radic S000004842RRP5YMR229CverifiedProtein required for the synthesis of both 18S and 5.8S rRNA;C-terminal region is crucial for the formationof 18S rRNA and N-terminal region is requiredfor the 5.8S rRNA; component of small ribosomalsubunit (SSU) processosome 0.006210.16563 S000006256NHP6AYPR052CverifiedHigh-mobility group non-histone chromatin protein, functionallyredundant with Nhp6Bp; homologous to mammalianhigh mobility group proteins 1 and 2; acts torecruit transcription factor Rcs1p to certainpromoters 0.006190.16491&radic&radic&radic S000000683YCR087WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene 0.006150.16432&radic&radic S000000816HEM14YER014WverifiedProtoporphyrinogen oxidase, a mitochondrial enzyme thatcatalyzes the seventh step in the hemebiosynthetic pathway, convertingprotoporphyrinogen IX to protoporphyrin IX 0.006170.16432&radic&radic&radic S000006378YPR174CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate 0.00610.16123&radic&radic S000001616YKL133CUncharacterizedHypothetical protein 0.006040.16123&radic&radic&radic S000000667IMG2YCR071CverifiedMitochondrial ribosomal protein of the small subunit 0.006120.16123&radic&radic S000002366GLE1YDL207WverifiedCytoplasmic nucleoporin required for polyadenylated RNA exportbut not for protein import; component of Nup82pnuclear pore subcomplex; contains a nuclearexport signal 0.006090.16123 S000002482PPH3YDR075WverifiedCatalytic subunit of an evolutionarily conserved proteinphosphatase complex containing Psy2p and theregulatory subunit Psy4p; required forcisplatin resistance; involved in activation ofGln3p 0.006060.16123&radic&radic&radic&radic S000003794RAV1YJR033CverifiedSubunit of the RAVE complex (Rav1p, Rav2p, Skp1p), whichpromotes assembly of the V-ATPase holoenzyme;required for transport between the early andlate endosome/PVC and for localization of TGNmembrane proteins; potential Cdc28p substrate 0.005970.15999&radic S000002505GRX3YDR098CverifiedHydroperoxide and superoxide-radical responsiveglutathione-dependent oxidoreductase; monothiolglutaredoxin subfamily member along with Grx4pand Grx5p; protects cells from oxidative damage 0.005960.15958&radic&radic&radic S000002796RVS167YDR388WverifiedActin-associated protein, subunit of a complex(Rvs161p-Rvs167p) involved in regulation ofactin cytoskeleton, endocytosis, and viabilityfollowing starvation or osmotic stress; homologof mammalian amphiphysin 0.005960.15958&radic&radic S000000165AST1YBL069WverifiedPeripheral membrane protein that interacts with the plasmamembrane ATPase Pma1p and has a role in itstargeting to the plasma membrane, possibly byinfluencing its incorporation into lipid rafts 0.005950.15958&radic&radic&radic&radic S000000456DUT1YBR252WverifieddUTPase, catalyzes hydrolysis of dUTP to dUMP and PPi, therebypreventing incorporation of uracil into DNAduring replication; critical for themaintenance of genetic stability 0.005930.15814&radic&radic&radic S000003140NUP49YGL172WverifiedSubunit of the Nsp1p-Nup57p-Nup49p-Nic96p subcomplex of thenuclear pore complex (NPC), required fornuclear export of ribosomes 0.005930.15814 S000006273SPE3YPR069CverifiedSpermidine synthase, involved in biosynthesis of spermidine andalso in biosynthesis of pantothenic acid;spermidine is required for growth of wild-typecells 0.005910.15814&radic&radic S000002636PCF11YDR228CverifiedmRNA 3' end processing factor, essential component of cleavageand polyadenylation factor IA (CF IA), involvedin pre-mRNA 3' end processing and intranscription termination; binds C-terminaldomain of largest subunit of RNA pol II(Rpo21p) 0.005870.1576&radic&radic&radic&radic S000005433THP1YOL072WverifiedNuclear pore-associated protein, forms a complex with Sac3pthat is involved in transcription and in mRNAexport from the nucleus; contains a PAM domainimplicated in protein-protein binding 0.005880.1576&radic&radic S000002545HPR1YDR138WverifiedSubunit of THO/TREX complexes that couple transcriptionelongation with mitotic recombination and withmRNA metabolism and export, subunit of an RNAPol II complex; regulates lifespan; involved intelomere maintenance; similar to Top1p 0.005790.15567&radic&radic&radic&radic S000002625RAD9YDR217CverifiedDNA damage-dependent checkpoint protein, required forcell-cycle arrest in G1/S, intra-S, and G2/M;transmits checkpoint signal by activatingRad53p and Chk1p; hyperphosphorylated by Mec1pand Tel1p; potential Cdc28p substrate 0.00570.15334&radic&radic&radic&radic&radic S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 0.005670.1528&radic&radic&radic&radic S000003175SPT16YGL207WverifiedSubunit of the heterodimeric FACT complex (Spt16p-Pob3p),facilitates RNA Polymerase II transcriptionelongation through nucleosomes by destabilizingand then reassembling nucleosome structure 0.005670.1528&radic&radic S000006339CTF4YPR135WverifiedChromatin-associated protein, required for sister chromatidcohesion; interacts with DNA polymerase alpha(Pol1p) and may link DNA synthesis to sisterchromatid cohesion 0.005680.1528&radic&radic&radic&radic S000001234CTF8YHR191CverifiedSubunit of a complex with Ctf18p that shares some subunits withReplication Factor C and is required for sisterchromatid cohesion 0.005660.15272&radic S000002843PPM1YDR435CverifiedCarboxyl methyl transferase, methylates the C terminus of theprotein phosphatase 2A catalytic subunit(Pph21p or Pph22p), which is important forcomplex formation with regulatory subunits 0.005640.15084&radic S000002299RPO21YDL140CverifiedRNA polymerase II largest subunit B220, part of central core;phosphorylation of C-terminal heptapeptiderepeat domain regulates association withtranscription and splicing factors; similar tobacterial beta-prime 0.005630.15084&radic S000003684RPA34YJL148WverifiedRNA polymerase I subunit A34.5 0.005610.15084&radic&radic S000000019CCR4YAL021CverifiedComponent of the CCR4-NOT transcriptional complex, which isinvolved in regulation of gene expression;component of the major cytoplasmic deadenylase,which is involved in mRNA poly(A) tailshortening 0.00560.15084&radic S000004871YMR258CUncharacterizedProtein of unknown function with similarity to F-box proteins; physically interacts with Skp1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR258C is not an essential gene 0.005610.15084&radic&radic&radic&radic S000005524BDS1YOL164WverifiedBacterially-derived sulfatase required for use of alkyl- andaryl-sulfates as sulfur sources 0.005640.15084 S000005442ATG19YOL082WverifiedProtein involved in the cytoplasm-to-vacuole targeting pathwayand in autophagy, recognizes cargo proteins anddelivers them to the preautophagosomalstructure for eventual engulfment by theautophagosome and degradation 0.005610.15084&radic&radic S000006257YPR053CDubiousHypothetical protein 0.005620.15084&radic&radic S000004294MET17YLR303WverifiedO-acetyl homoserine-O-acetyl serine sulfhydrylase, required forsulfur amino acid synthesis 0.005590.15009&radic&radic&radic&radic S000003084CDC20YGL116WverifiedCell-cycle regulated activator of anaphase-promotingcomplex/cyclosome (APC/C), which is requiredfor metaphase/anaphase transition; directsubiquitination of mitotic cyclins, Pds1p, andother anaphase inhibitors; potential Cdc28psubstrate 0.005590.15009&radic S000004280GUF1YLR289WverifiedMitochondrial GTPase of unknown function, similar to E. colielongation factor-type GTP-binding protein LepAand to LK1236.1 from Caenorhabditis elegans 0.005550.14955&radic&radic&radic&radic&radic&radic S000000167YBL071CDubiousHypothetical protein 0.00550.1479&radic&radic&radic&radic S000002553SWI5YDR146CverifiedTranscription factor that activates transcription of genesexpressed at the M/G1 phase boundary and in G1phase; localization to the nucleus occursduring G1 and appears to be regulated byphosphorylation by Cdc28p kinase 0.005430.14592&radic&radic&radic S000001333PCI8YIL071CverifiedPossible shared subunit of Cop9 signalosome (CSN) and eIF3,binds eIF3b subunit Prt1p, has possible dualfunctions in transcriptional and translationalcontrol, contains a PCI (Proteasome-COP9signalosome (CSN)-eIF3) domain 0.005430.14592&radic&radic&radic&radic S000005364SIN3YOL004WverifiedComponent of the Sin3p-Rpd3p histone deacetylase complex,involved in transcriptional repression andactivation of diverse processes, includingmating-type switching and meiosis; involved inthe maintenance of chromosomal integrity 0.005450.14592&radic S000002458YDR051CUncharacterizedHypothetical protein 0.005450.14592&radic&radic&radic&radic&radic S000005294PRP2YNR011CverifiedRNA-dependent ATPase in the DEAH-box family, required foractivation of the spliceosome before the firsttransesterification step in RNA splicing 0.005410.14592&radic&radic S000001767TIF1YKR059WverifiedTranslation initiation factor eIF4A, identical to Tif2p;DEA(D/H)-box RNA helicase that couples ATPaseactivity to RNA binding and unwinding; forms adumbbell structure of two compact domainsconnected by a linker; interacts with eIF4G 0.005390.14568&radic S000000441PRP5YBR237WverifiedRNA helicase in the DEAD-box family, necessary forprespliceosome formation, bridges U1 and U2snRNPs and enables stable U2 snRNP associationwith intron RNA 0.005390.14568&radic&radic&radic&radic S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus 0.005390.14568&radic&radic&radic&radic&radic S000002690YDR282CUncharacterizedHypothetical protein 0.005390.14519 S000005784HNT3YOR258WverifiedMember of the third branch of the histidine triad (HIT)superfamily of nucleotide-binding proteins;similar to Aprataxin, a Hint related proteinthat is mutated in individuals with ataxia withoculomotor apraxia 0.005330.14409&radic&radic&radic&radic&radic&radic S000001817YAL065CUncharacterizedHypothetical protein 0.005330.14409&radic&radic S000005398PRE6YOL038Wverified20S proteasome alpha-type subunit; green fluorescent protein(GFP)-fusion protein relocates from the cytosolto the mitochondrial outer surface uponoxidative stress 0.00530.14305&radic&radic S000005887PDE2YOR360CverifiedHigh-affinity cyclic AMP phosphodiesterase, component of thecAMP-dependent protein kinase signaling system,protects the cell from extracellular cAMP,contains readthrough motif surroundingtermination codon 0.005270.14244&radic&radic&radic S000003962UBI4YLL039CverifiedUbiquitin, becomes conjugated to proteins, marking them forselective degradation via the ubiquitin-26Sproteasome system; essential for the cellularstress response 0.005280.14244&radic&radic&radic&radic S000003026RAD6YGL058WverifiedUbiquitin-conjugating enzyme (E2), involved in postreplicationrepair (with Rad18p), sporulation, telomeresilencing, and ubiquitin-mediated N-end ruleprotein degradation (with Ubr1p) 0.005260.14178&radic&radic S000002984KAP122YGL016WverifiedKaryopherin beta, responsible for import of the Toa1p-Toa2pcomplex into the nucleus; binds to nucleoporinsNup1p and Nup2p; may play a role in regulationof pleiotropic drug resistance 0.005250.14178&radic&radic S000007255TIM11YDR322C-AverifiedSubunit e of mitochondrial F1F0-ATPase, which is a large,evolutionarily conserved enzyme complexrequired for ATP synthesis; essential for thedimeric state of ATP synthase 0.005240.14162&radic S000000330TPS1YBR126CverifiedSynthase subunit of trehalose-6-phosphate synthase/phosphatasecomplex, which synthesizes the storagecarbohydrate trehalose; also found in amonomeric form; expression is induced by thestress response and repressed by the Ras-cAMPpathway 0.005230.14141&radic&radic S000002470YDR063WUncharacterizedHypothetical protein 0.005220.14111&radic&radic&radic&radic&radic S000000760HYP2YEL034WverifiedTranslation initiation factor eIF-5A, promotes formation of thefirst peptide bond; similar to and functionallyredundant with Anb1p; possible role intranslation elongation; undergoes an essentialhypusination modification 0.005220.14111&radic S000002562CPR1YDR155CverifiedCytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin),catalyzes the cis-trans isomerization ofpeptide bonds N-terminal to proline residues;binds the drug cyclosporin A 0.005160.13915&radic&radic&radic&radic S000003295SPT4YGR063CverifiedProtein that forms a complex with Spt5p and mediates bothactivation and inhibition of transcriptionelongation, and plays a role in pre-mRNAprocessing; in addition, Spt4p is involved inkinetochore function and gene silencing 0.005150.13915&radic&radic&radic S000001337RPN2YIL075CverifiedSubunit of the 26S proteasome, substrate of theN-acetyltransferase Nat1p 0.00510.13718&radic S000005862ALA1YOR335CverifiedCytoplasmic alanyl-tRNA synthetase, required for proteinsynthesis; point mutation (cdc64-1 allele)causes cell cycle arrest at G1; lethality ofnull mutation is functionally complemented byhuman homolog 0.005090.13718&radic S000006368MMS1YPR164WverifiedProtein likely involved in protection againstreplication-dependent DNA damage; mutants aresensitive to methyl methanesulfonate (MMS);implicated in regulation of Ty1 transposition 0.005120.13718&radic S000002222UBC9YDL064WverifiedSUMO-conjugating enzyme involved in the Smt3p conjugationpathway; nuclear protein required for S- andM-phase cyclin degradation and mitotic control;involved in proteolysis mediated by theanaphase-promoting complex cyclosome (APCC) 0.005120.13718&radic&radic S000002524TMA64YDR117CverifiedProtein of unknown function that associates with ribosomes; hasa putative RNA binding domain 0.005090.13718&radic&radic&radic&radic S000000777VMA8YEL051WverifiedSubunit D of the eight-subunit V1 peripheral membrane domain ofthe vacuolar H+-ATPase (V-ATPase), anelectrogenic proton pump found throughout theendomembrane system; plays a role in thecoupling of proton transport and ATP hydrolysis 0.005130.13718&radic&radic S000005612TCB1YOR086CverifiedLipid-binding protein containing three calcium and lipidbinding domains; non-tagged protein localizesto mitochondria and GFP-fusion proteinlocalizes to the cell periphery; C-termini ofTcb1p, Tcb2p and Tcb3p interact 0.005020.13534&radic S000003245SNU71YGR013WverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; yeast specific, no metazoancounterpart 0.005030.13534&radic&radic S000002520PDS1YDR113CverifiedSecurin that inhibits anaphase by binding separin Esp1p, alsoblocks cyclin destruction and mitotic exit,essential for cell cycle arrest in mitosis inthe presence of DNA damage or aberrant mitoticspindles; also present in meiotic nuclei 0.005010.13511&radic&radic S000000339CKS1YBR135WverifiedSubunit of the Cdc28 protein kinase, required for mitoticproteolysis, may also be involved in theproteolysis of the G1 cyclins 0.004980.13424&radic S000003016RPT6YGL048CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; bound byubiquitin-protein ligases Ubr1p and Ufd4p;localized mainly to the nucleus throughout thecell cycle 0.004960.13329&radic S000003451YGR219WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C 0.004920.13197&radic&radic&radic&radic S000003059NBP35YGL091CverifiedEssential P-loop NTPase that functions in nuclear and cytosoliciron-sulfur (FeS) protein assembly; has ahighly conserved N-terminus 0.004940.13197&radic&radic&radic S000001410RPL40AYIL148WverifiedFusion protein, identical to Rpl40Bp, that is cleaved to yieldubiquitin and a ribosomal protein of the large(60S) ribosomal subunit with similarity to ratL40; ubiquitin may facilitate assembly of theribosomal protein into ribosomes 0.004910.13197&radic S000006132NIP7YPL211WverifiedNucleolar protein required for 60S ribosome subunit biogenesis,constituent of 66S pre-ribosomal particles;physically interacts with Nop8p and the exosomesubunit Rrp43p 0.004920.13197&radic&radic&radic S000006363KRE6YPR159WverifiedProtein required for beta-1,6 glucan biosynthesis; putativebeta-glucan synthase; appears functionallyredundant with Skn1p 0.004880.13122&radic&radic&radic S000005805RFM1YOR279CverifiedDNA-binding protein required for vegetative repression ofmiddle sporulation genes; specificity factorthat directs the Hst1p histone deacetylase tosome of the promoters regulated by Sum1p;involved in telomere maintenance 0.004840.12939&radic&radic&radic&radic S000001222OYE2YHR179WverifiedWidely conserved NADPH oxidoreductase containing flavinmononucleotide (FMN), homologous to Oye3p withslight differences in ligand binding andcatalytic properties; may be involved in sterolmetabolism 0.004860.12939&radic&radic&radic S000003450CRM1YGR218WverifiedMajor karyopherin, involved in export of proteins, RNAs, andribosomal subunits from the nucleus 0.004810.12934&radic&radic&radic S000005564HIR2YOR038CverifiedNon-essential transcriptional corepressor involved in the cellcycle-regulated transcription of histone H2A,H2B, H3, and H4 genes; recruits Swi-Snfcomplexes to histone gene promoters; promotesheterochromatic gene silencing with Asf1p 0.004790.12855&radic&radic S000002589SAS4YDR181CverifiedSubunit of the SAS complex (Sas2p, Sas4p, Sas5p), whichacetylates free histones and nucleosomes andregulates transcriptional silencing; requiredfor the HAT activity of Sas2p 0.004760.12744&radic&radic&radic&radic&radic S000005846HSH49YOR319WverifiedU2-snRNP associated splicing factor with similarity to themammalian splicing factor SAP49; proposed tofunction as a U2-snRNP assembly factor alongwith Hsh155p and binding partner Cus1p;contains two RNA recognition motifs (RRM) 0.004750.12744&radic&radic S000001073RRM3YHR031CverifiedDNA helicase involved in rDNA replication and Ty1transposition; relieves replication fork pausesat telomeric regions; structurally andfunctionally related to Pif1p 0.004770.12744&radic S000004384YLR392CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR392C is not an essential gene 0.004750.12744&radic&radic&radic&radic S000004218ECM22YLR228CverifiedSterol regulatory element binding protein, regulatestranscription of the sterol biosynthetic genesERG2 and ERG3; member of the fungus-specificZn[2]-Cys[6] binuclear cluster family oftranscription factors; homologous to Upc2p 0.004760.12744&radic&radic&radic&radic S000001578YJU2YKL095WverifiedEssential nuclear protein; putative spliceosomal componentinvolved in mRNA splicing, based oncomputational analysis of large-scaleprotein-protein interaction data 0.004760.12744&radic&radic&radic&radic&radic S000003956IRC19YLL033WuncharacterizedPutative protein of unknown function; YLL033W is not anessential gene but mutant is defective in sporeformation; null mutant displays increasedlevels of spontaneous Rad52 foci 0.004780.12744&radic&radic S000003336SRB5YGR104CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation; involved intelomere maintenance 0.004690.12576&radic&radic&radic&radic&radic S000004069SIC1YLR079WverifiedInhibitor of Cdc28-Clb kinase complexes that controls G1/Sphase transition, preventing premature S phaseand ensuring genomic integrity; phosphorylationtargets Sic1p for SCF(CDC4)-dependent turnover;functional homolog of mammalian Kip1 0.004710.12576&radic&radic&radic S000001722YPT52YKR014CverifiedGTPase, similar to Ypt51p and Ypt53p and to mammalian rab5;required for vacuolar protein sorting andendocytosis 0.004690.12515&radic&radic&radic S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 0.004680.12515&radic&radic&radic&radic S000002665SET7YDR257CverifiedNuclear protein that contains a SET-domain, which have beenshown to mediate methyltransferase activity inother proteins 0.004660.12474&radic&radic&radic S000001195SPO12YHR152WverifiedNucleolar protein of unknown function, positive regulator ofexit from mitosis; involved in regulating therelease of Cdc14p from the nucleolus in earlyanaphase; proposed to play similar role inmeiosis 0.004640.12381&radic&radic&radic S000005159IES2YNL215WverifiedProtein that associates with the INO80 chromatin remodelingcomplex under low-salt conditions 0.004650.12381&radic&radic&radic&radic&radic S000001526PHD1YKL043WverifiedTranscriptional activator that enhances pseudohyphal growth;regulates expression of FLO11, an adhesinrequired for pseudohyphal filament formation;similar to StuA, an A. nidulans developmentalregulator; potential Cdc28p substrate 0.004630.12381&radic&radic&radic&radic S000001302APQ12YIL040WverifiedProtein involved in nucleocytoplasmic transport of mRNA 0.004650.12381&radic&radic S000002201PRP11YDL043CverifiedSubunit of the SF3a splicing factor complex, required forspliceosome assembly 0.004620.1232&radic&radic&radic S000002536SAC6YDR129CverifiedFimbrin, actin-bundling protein; cooperates with Scp1p(calponin/transgelin) in the organization andmaintenance of the actin cytoskeleton 0.004610.1232&radic S000003582YJL046WUncharacterizedPutative protein of unknown function, predicted to encode a lipoate-protein ligase A family member; deletion mutant has a respiratory growth defect 0.004620.1232&radic&radic S000005650UBP2YOR124CverifiedUbiquitin-specific protease that removes ubiquitin fromubiquitinated proteins, cleaves at the Cterminus of ubiquitin fusions; capable ofcleaving polyubiquitin and possessesisopeptidase activity 0.00460.12299&radic&radic S000003730CDC6YJL194WverifiedEssential ATP-binding protein required for DNA replication,component of the pre-replicative complex(pre-RC) which requires ORC to associate withchromatin and is in turn required for Mcm2-7pDNA association; homologous to S. pombe Cdc18p 0.00460.12251&radic&radic S000002580ARG82YDR173CverifiedInositol polyphosphate multikinase (IPMK), sequentiallyphosphorylates Ins(1,4,5)P3 to formIns(1,3,4,5,6)P5; putative nuclear lipidinositol PI3-kinase; regulates arginine-,phosphate-, and nitrogen-responsive genes 0.004580.12201&radic&radic&radic S000004306CDC3YLR314CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM 0.004580.12201&radic&radic S000003457AMA1YGR225WverifiedActivator of meiotic anaphase promoting complex (APC/C); Cdc20pfamily member; required for initiation of sporewall assembly; required for Clb1p degradationduring meiosis 0.004550.12105&radic&radic S000003567MAD2YJL030WverifiedComponent of the spindle-assembly checkpoint complex, whichdelays the onset of anaphase in cells withdefects in mitotic spindle assembly; forms acomplex with Mad1p 0.004530.12053&radic&radic S000002696NSE3YDR288WuncharacterizedEssential subunit of the Mms21-Smc5-Smc6 complex; protein ofunknown function; required for DNA repair andgrowth 0.004480.11865&radic&radic&radic&radic S000000062SEO1YAL067CverifiedPutative permease, member of the allantoate transportersubfamily of the major facilitator superfamily;mutation confers resistance to ethioninesulfoxide 0.004510.11865&radic&radic S000000814PRE1YER012Wverified20S proteasome beta-type subunit; localizes to the nucleusthroughout the cell cycle 0.00450.11865 S000002574TAF10YDR167WverifiedSubunit (145 kDa) of TFIID and SAGA complexes, involved in RNApolymerase II transcription initiation and inchromatin modification 0.00450.11865&radic&radic&radic&radic S000002435REG1YDR028CverifiedRegulatory subunit of type 1 protein phosphatase Glc7p,involved in negative regulation ofglucose-repressible genes 0.004450.11776&radic S000005664RUP1YOR138CverifiedProtein involved in regulation of Rsp5p, which is an essentialHECT ubiquitin ligase; required for binding ofRsp5p to Ubp2p; contains an UBA domain 0.004430.11721&radic&radic&radic S000000001TFC3YAL001CverifiedLargest of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauB domain of TFIIIC that binds DNA at theBoxB promoter sites of tRNA and similar genes;cooperates with Tfc6p in DNA binding 0.004430.11721&radic&radic&radic S000004889DSK2YMR276WverifiedNuclear-enriched ubiquitin-like polyubiquitin-binding protein,required for spindle pole body (SPB)duplication and for transit through the G2/Mphase of the cell cycle, involved inproteolysis, interacts with the proteasome 0.004420.11665&radic&radic&radic S000000521DCC1YCL016CverifiedSubunit of a complex with Ctf8p and Ctf18p that shares somecomponents with Replication Factor C, requiredfor sister chromatid cohesion and telomerelength maintenance 0.004410.11652&radic&radic&radic S000002615UME6YDR207CverifiedKey transcriptional regulator of early meiotic genes, bindsURS1 upstream regulatory sequence, couplesmetabolic responses to nutritional cues withinitiation and progression of meiosis, formscomplex with Ime1p, and also with Sin3p-Rpd3p 0.00440.11643&radic&radic S000000773YEL047CVerifiedFumurate ReDuctase Soluble 0.004380.11546&radic&radic&radic&radic&radic S000001521RGT1YKL038WverifiedGlucose-responsive transcription factor that regulatesexpression of several glucose transporter (HXT)genes in response to glucose; binds topromoters and acts both as a transcriptionalactivator and repressor 0.004390.11546&radic&radic S000003174CHC1YGL206CverifiedClathrin heavy chain, subunit of the major coat proteininvolved in intracellular protein transport andendocytosis; two heavy chains form the clathrintriskelion structural component; the lightchain (CLC1) is thought to regulate function 0.004390.11546 S000002744YDR336WUncharacterizedPutative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene 0.004390.11546&radic&radic&radic&radic S000005872YOR345CDubiousHypothetical protein 0.004360.11417&radic S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.004350.11417&radic S000003358YGR126WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 0.004330.11391&radic&radic&radic S000000302MMS4YBR098WverifiedSubunit of the structure-specific Mms4p-Mus81p endonucleasethat cleaves branched DNA; involved inrecombination and DNA repair 0.004330.11391&radic&radic&radic S000004603CDC5YMR001CverifiedPolo-like kinase with similarity to Xenopus Plx1 and S. pombePlo1p; found at bud neck, nucleus and SPBs; hasmultiple functions in mitosis and cytokinesisthrough phosphorylation of substrates; may be aCdc28p substrate 0.004310.11313&radic S000004624QRI8YMR022WverifiedUbiquitin conjugating enzyme, involved in the ER-associatedprotein degradation pathway; requires Cue1p forrecruitment to the ER membrane; proposed to beinvolved in chromatin assembly 0.004310.11313&radic&radic S000001334HOP1YIL072WverifiedMeiosis-specific DNA binding protein that displays Red1pdependent localization to the unsynapsedaxial-lateral elements of the synaptonemalcomplex; required for homologous chromosomesynapsis and chiasma formation 0.004330.11313&radic&radic&radic&radic&radic S000004614CLU1YMR012WverifiedeIF3 component of unknown function; deletion causes defects inmitochondrial organization but not in growth ortranslation initiation, can rescue cytokinesisand mitochondrial organization defects of theDictyostelium cluA- mutant 0.004270.11219&radic&radic S000002973COG7YGL005CverifiedComponent of the conserved oligomeric Golgi complex (Cog1pthrough Cog8p), a cytosolic tethering complexthat functions in protein trafficking tomediate fusion of transport vesicles to Golgicompartments 0.004220.11016&radic&radic&radic&radic S000004096MDN1YLR106CverifiedHuge dynein-related AAA-type ATPase (midasin), forms extendedpre-60S particle with the Rix1 complex(Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependentremodeling of 60S subunits and subsequentexport from nucleoplasm to cytoplasm 0.004210.11016&radic&radic&radic&radic&radic S000004584ATR1YML116WverifiedMultidrug efflux pump of the major facilitator superfamily,required for resistance to aminotriazole and4-nitroquinoline-N-oxide 0.004220.11016 S000001082BCD1YHR040WverifiedEssential protein required for the accumulation of box C/DsnoRNA 0.004220.11016&radic&radic&radic S000005295URK1YNR012WverifiedUridine/cytidine kinase, component of the pyrimidineribonucleotide salvage pathway that convertsuridine into UMP and cytidine into CMP;involved in the pyrimidine deoxyribonucleotidesalvage pathway, converting deoxycytidine intodCMP 0.004210.10971&radic&radic S000005721SLK19YOR195WverifiedKinetochore-associated protein required for normal segregationof chromosomes in meiosis and mitosis;component of the FEAR regulatory network, whichpromotes Cdc14p release from the nucleolusduring anaphase; potential Cdc28p substrate 0.00420.10936&radic&radic&radic S000002142PAU8YAL068CuncharacterizedHypothetical protein 0.004190.10936&radic S000002610RAV2YDR202CverifiedSubunit of RAVE (Rav1p, Rav2p, Skp1p), a complex thatassociates with the V1 domain of the vacuolarmembrane (H+)-ATPase (V-ATPase) and promotesassembly and reassembly of the holoenzyme 0.004190.10908&radic&radic S000005787RPN8YOR261CverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome;has similarity to the human p40 proteasomalsubunit and to another S. cerevisiae regulatorysubunit, Rpn11p 0.004180.10902&radic S000001444IST3YIR005WverifiedComponent of the U2 snRNP, required for the first catalyticstep of splicing and for spliceosomal assembly;interacts with Rds3p and is required forMer1p-activated splicing 0.004180.10902&radic&radic&radic&radic S000005430NUF2YOL069WverifiedComponent of the evolutionarily conservedkinetochore-associated Ndc80 complex(Ndc80p-Nuf2p-Spc24p-Spc25p); involved inchromosome segregation, spindle checkpointactivity and kinetochore clustering 0.004160.1085&radic&radic S000005394SMC5YOL034WverifiedStructural maintenance of chromosomes (SMC) protein; essentialsubunit of the Mms21-Smc5-Smc6 complex;required for growth and DNA repair; S. pombehomolog forms a heterodimer with S. pombeRad18p that is involved in DNA repair 0.004170.1085&radic&radic&radic S000000286UBC4YBR082CverifiedUbiquitin-conjugating enzyme that mediates degradation ofshort-lived and abnormal proteins; interactswith E3-CaM in ubiquitinating calmodulin;interacts with many SCF ubiquitin proteinligases; component of the cellular stressresponse 0.004140.10771&radic S000000231YBR027CDubiousHypothetical protein 0.004140.10771&radic S000004888BUL1YMR275CverifiedUbiquitin-binding component of the Rsp5p E3-ubiquitin ligasecomplex, functional homolog of Bul2p,disruption causes temperature-sensitive growth,overexpression causes missorting of amino acidpermeases 0.004140.10771&radic S000006161HSP82YPL240CverifiedCytoplasmic chaperone (Hsp90 family) required for pheromonesignaling and negative regulation of Hsf1p;docks with the mitochondrial import receptorTom70p for preprotein delivery; interacts withco-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p 0.004130.10731&radic S000005370RCL1YOL010WverifiedRNA terminal phosphate cyclase-like protein involved in rRNAprocessing at sites A0, A1, and A2; does notpossess detectable RNA cyclase activity 0.004130.10731&radic&radic&radic S000003372CBF2YGR140WverifiedEssential kinetochore protein, component of the CBF3multisubunit complex that binds to the CDEIIIregion of the centromere; Cbf2p also binds tothe CDEII region possibly forming a differentmultimeric complex, ubiquitinated in vivo 0.004090.10614&radic&radic&radic&radic S000004778CEP3YMR168CverifiedEssential kinetochore protein, component of the CBF3 complexthat binds the CDEIII region of the centromere;contains an N-terminal Zn2Cys6 type zinc fingerdomain, a C-terminal acidic domain, and aputative coiled coil dimerization domain 0.00410.10614&radic&radic S000003325YGR093WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus 0.004090.10614&radic&radic&radic S000001311DFG10YIL049WverifiedProtein of unknown function, involved in filamentous growth 0.00410.10614&radic&radic&radic&radic S000003517ZUO1YGR285CverifiedCytosolic ribosome-associated chaperone that acts, togetherwith Ssz1p and the Ssb proteins, as a chaperonefor nascent polypeptide chains; contains a DnaJdomain and functions as a J-protein partner forSsb1p and Ssb2p 0.00410.10614 S000004669KAR5YMR065WverifiedProtein required for nuclear membrane fusion during karyogamy,localizes to the membrane with a solubleportion in the endoplasmic reticulum lumen, mayform a complex with Jem1p and Kar2p; expressionof the gene is regulated by pheromone 0.004120.10614&radic&radic&radic S000002602MSS116YDR194CverifiedDEAD-box protein required for efficient splicing ofmitochondrial Group I and II introns; non-polarRNA helicase that also facilities strandannealing 0.004090.10608&radic&radic&radic&radic S000001886SMC1YFL008WverifiedSubunit of the multiprotein cohesin complex, essential proteininvolved in chromosome segregation and indouble-strand DNA break repair; SMC chromosomalATPase family member, binds DNA with apreference for DNA with secondary structure 0.004080.10569&radic&radic&radic&radic S000002922YDR514CUncharacterizedHypothetical protein 0.004070.10507&radic&radic S000001283RPB3YIL021WverifiedRNA polymerase II third largest subunit B44, part of centralcore; similar to prokaryotic alpha subunit 0.004070.10507&radic&radic S000003464NAS6YGR232WverifiedRegulatory, non-ATPase subunit of the 26S proteasome; homologof the human oncoprotein gankyrin, whichinteracts with the retinoblastoma tumorsuppressor (Rb) and cyclin-dependent kinase 4/6 0.004070.10507&radic&radic&radic S000003284FMP48YGR052WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.004070.10507&radic&radic S000004141PCD1YLR151CverifiedPeroxisomal nudix pyrophosphatase with specificity for coenzymeA and CoA derivatives, may function to removepotentially toxic oxidized CoA disulfide fromperoxisomes to maintain the capacity forbeta-oxidation of fatty acids 0.004070.10507&radic&radic&radic&radic S000002314CLB3YDL155WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the G2/M transition; may beinvolved in DNA replication and spindleassembly; accumulates during S phase and G2,then targeted for ubiquitin-mediateddegradation 0.004060.10507&radic&radic S000003507RTT102YGR275WverifiedComponent of both the SWI/SNF and RSC chromatin remodelingcomplexes, suggested role in chromosomemaintenance; possible weak regulator of Ty1transposition 0.004010.10321&radic&radic S000004216BUR2YLR226WverifiedCyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2pcomprise a CDK-cyclin complex involved intranscriptional regulation through itsphosphorylation of the carboxy-terminal domainof the largest subunit of RNA polymerase II 0.0040.10321&radic&radic&radic&radic&radic S000004370SEC61YLR378CverifiedEssential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p);forms a channel for SRP-dependent proteinimport and retrograde transport of misfoldedproteins out of the ER; with Sec63 complexallows SRP-independent protein import into ER 0.004020.10321&radic S000001537DEF1YKL054CverifiedRNAPII degradation factor, forms a complex with Rad26p inchromatin, enables ubiquitination andproteolysis of RNAPII present in an elongationcomplex 0.0040.10321&radic S000003472PFK1YGR240CverifiedAlpha subunit of heterooctameric phosphofructokinase involvedin glycolysis, indispensable for anaerobicgrowth, activated by fructose-2,6-bisphosphateand AMP, mutation inhibits glucose induction ofcell cycle-related genes 0.0040.10319&radic&radic S000003704SET2YJL168CverifiedHistone methyltransferase with a role in transcriptionalelongation, methylates a lysine residue ofhistone H3; associates with the C-terminaldomain of Rpo21p; histone methylation activityis regulated by phosphorylation status ofRpo21p 0.003990.10219&radic&radic&radic S000003516ERV29YGR284CverifiedProtein localized to COPII-coated vesicles, involved in vesicleformation and incorporation of specificsecretory cargo 0.003990.10219&radic&radic S000001863HAC1YFL031WverifiedbZIP transcription factor (ATF/CREB1 homolog) that regulatesthe unfolded protein response, via UPREbinding, and membrane biogenesis; ERstress-induced splicing pathway utilizingIre1p, Trl1p and Ada5p facilitates efficientHac1p synthesis 0.003990.10219&radic&radic&radic&radic S000004732STO1YMR125WverifiedLarge subunit of the nuclear mRNA cap-binding protein complex,interacts with Npl3p to carry nuclear poly(A)+mRNA to cytoplasm; also involved in nuclearmRNA degradation and telomere maintenance;orthologous to mammalian CBP80 0.003980.10219&radic&radic S000006331YPR127WUncharacterizedHypothetical protein 0.003960.10181&radic&radic&radic&radic S000001602VPH2YKL119CverifiedIntegral membrane protein required for vacuolar H+-ATPase(V-ATPase) function, although not an actualcomponent of the V-ATPase complex; functions inthe assembly of the V-ATPase; localized to theendoplasmic reticulum (ER) 0.003930.10036&radic S000004840TAF7YMR227CverifiedTFIID subunit (67 kDa), involved in RNA polymerase IItranscription initiation 0.003940.10036&radic&radic&radic&radic S000005785RPT4YOR259CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for spindlepole body duplication; localized mainly to thenucleus throughout the cell cycle 0.003930.10036&radic&radic&radic S000001769KTR2YKR061WverifiedMannosyltransferase involved in N-linked protein glycosylation;member of the KRE2/MNT1 mannosyltransferasefamily 0.003930.10036&radic&radic&radic&radic&radic S000001374HOS4YIL112WuncharacterizedSubunit of the Set3 complex, which is a meiotic-specificrepressor of sporulation specific genes thatcontains deacetylase activity; potential Cdc28psubstrate 0.003920.10036&radic&radic S000004121ACE2YLR131CverifiedTranscription factor that activates expression of earlyG1-specific genes, localizes to daughter cellnuclei after cytokinesis and delays G1progression in daughters, localization isregulated by phosphorylation; potential Cdc28psubstrate 0.003940.10036&radic&radic&radic&radic S000003726RPS22AYJL190CverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps22Bp and has similarity to E.coli S8 and rat S15a ribosomal proteins 0.003950.10036&radic&radic&radic S000001127IPI1YHR085WverifiedProtein of unknown function, essential for viability, may beinvolved in rRNA processing 0.003940.10036&radic&radic&radic&radic&radic S000003058LIF1YGL090WverifiedProtein involved in DNA double-strand break repair; physicallyinteracts with DNA ligase 4 (Lig4p); homologousto mammalian XRCC4 protein 0.003950.10036&radic&radic&radic S000002611YDR203WDubiousHypothetical protein 0.003920.10029&radic&radic&radic&radic&radic S000000104HIR1YBL008WverifiedNon-essential transcriptional corepressor involved in the cellcycle-regulated transcription of histone H2A,H2B, H3 and H4 genes; contributes to nucleosomeformation, heterochromatic gene silencing, andformation of functional kinetochores 0.00390.09928&radic&radic S000005897MRS6YOR370CverifiedRab escort protein, forms a complex with the Ras-like smallGTPase Ypt1p that is required for theprenylation of Ypt1p by proteingeranylgeranyltransferase type II (Bet2p-Bet4p) 0.003910.09928&radic&radic&radic S000005554CIN5YOR028CverifiedBasic leucine zipper transcriptional factor of the yAP-1 familythat mediates pleiotropic drug resistance andsalt tolerance; localizes constitutively to thenucleus 0.00390.09928&radic&radic&radic&radic S000004391BDF1YLR399CverifiedProtein involved in transcription initiation at TATA-containingpromoters; associates with the basaltranscription factor TFIID; contains twobromodomains; corresponds to the C-terminalregion of mammalian TAF1; redundant with Bdf2p 0.00390.09928&radic&radic S000001871BUD27YFL023WverifiedProtein involved in bud-site selection, nutrient signaling, andgene expression controlled by the TOR kinase;diploid mutants display a random buddingpattern instead of the wild-type bipolarpattern 0.003890.09869&radic&radic&radic S000005307YNR024WUncharacterizedHypothetical protein 0.003870.09836&radic&radic&radic&radic S000005117MDG1YNL173CverifiedPlasma membrane protein involved in G-protein mediatedpheromone signaling pathway; overproductionsuppresses bem1 mutations 0.003860.09707&radic&radic&radic S000005900NUD1YOR373WverifiedComponent of the spindle pole body outer plaque, required forexit from mitosis 0.003840.09707&radic S000003411OKP1YGR179CverifiedOuter kinetochore protein, required for accurate mitoticchromosome segregation; component of thekinetochore sub-complex COMA (Ctf19p, Okp1p,Mcm21p, Ame1p) that functions as a platform forkinetochore assembly 0.003840.09707&radic&radic S000002546RUB1YDR139CverifiedUbiquitin-like protein with similarity to mammalian NEDD8;conjugation (neddylation) substrates includethe cullins Cdc53p, Rtt101p, and Cul3p;activated by Ula1p and Uba3p (E1 enzyme pair);conjugation mediated by Ubc12p (E2 enzyme) 0.003860.09707&radic&radic&radic S000003963VPS13YLL040CverifiedProtein of unknown function; heterooligomeric or homooligomericcomplex; peripherally associated withmembranes; homologous to human COH1; involvedin sporulation, vacuolar protein sorting andprotein-Golgi retention 0.003850.09707&radic&radic S000000331VMA2YBR127CverifiedSubunit B of the eight-subunit V1 peripheral membrane domain ofthe vacuolar H+-ATPase (V-ATPase), anelectrogenic proton pump found throughout theendomembrane system; contains nucleotidebinding sites; also detected in the cytoplasm 0.003840.09707 S000000287TEC1YBR083WverifiedTranscription factor required for full Ty1 epxression,Ty1-mediated gene activation, and haploidinvasive and diploid pseudohyphal growth;TEA/ATTS DNA-binding domain family member 0.003860.09707&radic&radic S000000452HIS7YBR248CverifiedImidazole glycerol phosphate synthase (glutamineamidotransferase:cyclase), catalyzes the fifthand sixth steps of histidine biosynthesis andalso produces 5-aminoimidazole-4-carboxamideribotide (AICAR), a purine precursor 0.003830.09671&radic&radic S000003103RPL1BYGL135WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl1Apand has similarity to E. coli L1 and rat L10aribosomal proteins; rpl1a rpl1b double nullmutation is lethal 0.003820.09671&radic S000003617ARP4YJL081CverifiedNuclear actin-related protein involved in chromatin remodeling,component of chromatin-remodeling enzymecomplexes 0.003820.09624&radic&radic S000006271ISA2YPR067WverifiedProtein required for maturation of mitochondrial and cytosolicFe/S proteins, localizes to the mitochondrialintermembrane space, overexpression of ISA2suppresses grx5 mutations 0.003810.09624&radic S000001507URA6YKL024CverifiedUridylate kinase, catalyzes the seventh enzymatic step in thede novo biosynthesis of pyrimidines, convertinguridine monophosphate (UMP) intouridine-5'-diphosphate (UDP) 0.003810.09599&radic&radic S000002742SWR1YDR334WverifiedSwi2/Snf2-related ATPase that is the structural component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.003790.09542&radic&radic&radic&radic S000004271YLR281CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene 0.003790.09542&radic&radic&radic&radic S000006098CUP9YPL177CverifiedHomeodomain-containing transcriptional repressor of PTR2, whichencodes a major peptide transporter; importedpeptides activate ubiquitin-dependentproteolysis, resulting in degradation of Cup9pand de-repression of PTR2 transcription 0.00380.09542&radic&radic S000001318VHR1YIL056WuncharacterizedTranscriptional activator, required for the vitaminH-responsive element (VHRE) mediated inductionof VHT1 (Vitamin H transporter) and BIO5(biotin biosynthesis intermediate transporter)in response to low biotin concentrations 0.003790.0954&radic&radic S000005773SRL1YOR247WverifiedMannoprotein that exhibits a tight association with the cellwall, required for cell wall stability in theabsence of GPI-anchored mannoproteins; has ahigh serine-threonine content; expression isinduced in cell wall mutants 0.003780.09479&radic&radic S000000494BSD2YBR290WverifiedHeavy metal ion homeostasis protein, facilitates trafficking ofSmf1p and Smf2p metal transporters to thevacuole where they are degraded, controls metalion transport, prevents metalhyperaccumulation, functions in copperdetoxification 0.003770.09384 S000003352COG2YGR120CverifiedEssential component of the conserved oligomeric Golgi complex(Cog1p through Cog8p), a cytosolic tetheringcomplex that functions in protein traffickingto mediate fusion of transport vesicles toGolgi compartments 0.003760.09384&radic&radic S000001628RPT1YKL145WverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for optimalCDC20 transcription; interacts with Rpn12p andthe E3 ubiquitin-protein ligase Ubr1p 0.003750.09384&radic&radic&radic S000006103YPL182CDubiousHypothetical protein 0.003750.09384&radic&radic S000002725HIM1YDR317WverifiedProtein of unknown function involved in DNA repair 0.003760.09384&radic&radic S000003725RPL39YJL189WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to rat L39 ribosomal protein;required for ribosome biogenesis; exhibitsgenetic interactions with SIS1 and PAB1 0.003750.09384&radic S000004511GSF2YML048WverifiedER localized integral membrane protein that may promotesecretion of certain hexose transporters,including Gal2p; involved in glucose-dependentrepression 0.003760.09384&radic S000004839TMA29YMR226CverifiedNADP(+)-dependent dehydrogenase; acts on serine,L-allo-threonine, and other 3-hydroxy acids;green fluorescent protein fusion proteinlocalizes to the cytoplasm and nucleus; mayinteract with ribosomes, based onco-purification experiments 0.003740.09349&radic&radic&radic&radic S000002940YDR532CUncharacterizedProtein of unknown function that localizes to the nuclear side of the spindle pole body and along short spindles; deletion mutants have short telomeres; forms a complex with Spc105p 0.003740.09349&radic&radic S000005080EAF7YNL136WverifiedSubunit of the NuA4 histone acetyltransferase complex, whichacetylates the N-terminal tails of histones H4and H2A 0.003720.09314&radic&radic S000005450MSH2YOL090WverifiedProtein that forms heterodimers with Msh3p and Msh6p that bindto DNA mismatches to initiate the mismatchrepair process; contains a Walker ATP-bindingmotif required for repair activity; Msh2p-Msh6pbinds to and hydrolyzes ATP 0.003730.09314&radic&radic&radic&radic S000006191MDL2YPL270WverifiedHalf-type ATP-binding cassette (ABC) transporter of the innermitochondrial membrane 0.003710.09265&radic&radic&radic&radic S000002259DUN1YDL101CverifiedCell-cycle checkpoint serine-threonine kinase required for DNAdamage-induced transcription of certain targetgenes, phosphorylation of Rad55p and Sml1p, andtransient G2/M arrest after DNA damage; alsoregulates postreplicative DNA repair 0.003710.09218&radic&radic S000004312MMS22YLR320WverifiedProtein involved in resistance to ionizing radiation; acts withMms1p in a repair pathway that may be involvedin resolving replication intermediates orpreventing the damage caused by blockedreplication forks 0.00370.09218&radic&radic S000006391RPO26YPR187WverifiedRNA polymerase subunit ABC23, common to RNA polymerases I, II,and III; part of central core; similar tobacterial omega subunit 0.003690.09176&radic&radic&radic S000002581HMO1YDR174WverifiedChromatin associated high mobility group (HMG) family memberinvolved in genome maintenance; rDNA-bindingcomponent of the Pol I transcription system;associates with a 5'-3' DNA helicase and Fpr1p,a prolyl isomerase 0.003690.09176&radic S000002720SSF2YDR312WverifiedProtein required for ribosomal large subunit maturation,functionally redundant with Ssf1p; member ofthe Brix family 0.003690.09176&radic&radic S000003104MRM2YGL136CverifiedMitochondrial 21S rRNA methyltransferase, required formethylation of U(2791) in 21S rRNA; MRM2deletion confers thermosensitive respirationand loss of mitochondrial DNA; has similarityto Spb1p and Trm7p, and to E. coli FtsJ/RrmJ 0.003690.09176&radic S000001672HYM1YKL189WverifiedComponent of the RAM signaling network that is involved inregulation of Ace2p activity and cellularmorphogenesis, interacts with Kic1p and Sog2p,localizes to sites of polarized growth duringbudding and during the mating response 0.003690.09176&radic&radic&radic S000004116YLR126CUncharacterizedPutative protein of unknown function with similarity to glutamine amidotransferase proteins; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein 0.003690.09176&radic&radic&radic S000002793EFT2YDR385WverifiedElongation factor 2 (EF-2), also encoded by EFT1; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 0.003690.09176&radic&radic S000003849BIR1YJR089WverifiedEssential chromosomal passenger protein involved incoordinating cell cycle events for properchromosome segregation; C-terminal region bindsSli15p, and the middle region, uponphosphorylation, localizes Cbf2p to the spindleat anaphase 0.003670.09105&radic&radic S000003709RFA3YJL173CverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 0.003680.09105&radic&radic&radic S000005859VMA4YOR332WverifiedSubunit E of the eight-subunit V1 peripheral membrane domain ofthe vacuolar H+-ATPase (V-ATPase), anelectrogenic proton pump found throughout theendomembrane system; required for the V1 domainto assemble onto the vacuolar membrane 0.003680.09105&radic&radic S000003992NOC3YLR002CverifiedProtein that forms a nuclear complex with Noc2p that binds to66S ribosomal precursors to mediate theirintranuclear transport; also binds to chromatinto promote the association of DNA replicationfactors and replication initiation 0.003680.09105&radic&radic S000004841MTF1YMR228WverifiedMitochondrial RNA polymerase specificity factor with structuralsimilarity to S-adenosylmethionine-dependentmethyltransferases and functional similarity tobacterial sigma-factors, interacts withmitochondrial core polymerase Rpo41p 0.003680.09105&radic&radic&radic S000003143SAE2YGL175CverifiedProtein with a role in accurate meiotic and mitoticdouble-strand break repair; phosphorylated inresponse to DNA damage and required for normalresistance to DNA-damaging agents 0.003680.09105&radic&radic S000006307PRE2YPR103Wverified20S proteasome beta-type subunit, responsible for thechymotryptic activity of the proteasome 0.003670.09105&radic S000004072SRL2YLR082CverifiedProtein of unknown function; overexpression suppresses thelethality caused by a rad53 null mutation 0.003670.09105&radic&radic&radic&radic S000001622CTK1YKL139WverifiedCatalytic (alpha) subunit of C-terminal domain kinase I(CTDK-I), which phosphorylates the C-terminalrepeated domain of the RNA polymerase II largesubunit (Rpo21p) to affect both transcriptionand pre-mRNA 3' end processing 0.003670.09105&radic&radic&radic&radic S000005131YNL187WUncharacterizedPutative protein of unknown function; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; may interact with or be fuctionally redundant with Prp40p 0.003680.09105&radic&radic&radic&radic S000003860YUH1YJR099WverifiedUbiquitin C-terminal hydrolase that cleaves ubiquitin-proteinfusions to generate monomeric ubiquitin;hydrolyzes the peptide bond at the C-terminusof ubiquitin; also the major processing enzymefor the ubiquitin-like protein Rub1p 0.003660.09084&radic S000001235YHR192WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.003650.0896&radic S000000098HTB2YBL002WverifiedOne of two nearly identical (see HTB1) histone H2B subtypesrequired for chromatin assembly and chromosomefunction; Rad6p-Bre1p-Lge1p mediatedubiquitination regulates transcriptionalactivation, meiotic DSB formation and H3methylation 0.003640.0896&radic S000000284SEC18YBR080CverifiedATPase required for the release of Sec17p during the 'priming'step in homotypic vacuole fusion and for ER toGolgi transport; homolog of the mammalian NSF 0.003660.0896&radic S000001511TFA1YKL028WverifiedTFIIE large subunit, involved in recruitment of RNA polymeraseII to the promoter, activation of TFIIH, andpromoter opening 0.003620.0896&radic&radic S000006267YPR063CUncharacterizedHypothetical protein 0.003630.0896&radic S000004329VRP1YLR337CverifiedProline-rich actin-associated protein involved in cytoskeletalorganization and cytokinesis; related tomammalian Wiskott-Aldrich syndrome protein(WASP)-interacting protein (WIP) 0.003630.0896 S000004300IMH1YLR309CverifiedProtein involved in vesicular transport, mediates transportbetween an endosomal compartment and the Golgi,contains a Golgi-localization (GRIP) domainthat interacts with activated Arl1p-GTP tolocalize Imh1p to the Golgi 0.003650.0896&radic&radic&radic S000001111RRP4YHR069CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp43p and Dis3p 0.003650.0896&radic&radic&radic&radic&radic S000003463PHB2YGR231CverifiedSubunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDaring-shaped inner mitochondrial membranechaperone that stabilizes newly synthesizedproteins; determinant of replicative life span;involved in mitochondrial segregation 0.003660.0896&radic&radic S000005853MYO2YOR326WverifiedOne of two type V myosin motors (along with MYO4) involved inactin-based transport of cargos; required forthe polarized delivery of secretory vesicles,the vacuole, late Golgi elements, peroxisomes,and the mitotic spindle 0.003630.0896&radic S000002638YDR230WDubiousHypothetical protein 0.003630.0896&radic&radic&radic S000004846YMR233WUncharacterizedProtein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; YMR233W is not an essential gene 0.003630.0896&radic&radic&radic&radic S000002260CDC2YDL102WverifiedCatalytic subunit of DNA polymerase delta; required forchromosomal DNA replication during mitosis andmeiosis, intragenic recombination, repair ofdouble strand DNA breaks, and DNA replicationduring nucleotide excision repair (NER) 0.003640.0896&radic S000004765TPP1YMR156CverifiedDNA 3'-phosphatase that functions in repair of endogenousdamage of double-stranded DNA, activity isspecific for removal of 3' phosphates at strandbreaks; has similarity to the l-2-haloaciddehalogenase superfamily 0.003610.08915&radic&radic&radic&radic S000002187ARP2YDL029WverifiedEssential component of the Arp2/3 complex, which is a highlyconserved actin nucleation center required forthe motility and integrity of actin patches;involved in endocytosis and membrane growth andpolarity 0.003610.08866&radic S000001503SPT23YKL020CverifiedER membrane protein involved in regulation of OLE1transcription, acts with homolog Mga2p;inactive ER form dimerizes and one subunit isthen activated byubiquitin/proteasome-dependent processingfollowed by nuclear targeting 0.003610.08866&radic&radic S000004523SML1YML058WverifiedRibonucleotide reductase inhibitor involved in regulating dNTPproduction; regulated by Mec1p and Rad53pduring DNA damage and S phase 0.00360.08866&radic S000002457TPI1YDR050CverifiedTriose phosphate isomerase, abundant glycolytic enzyme; mRNAhalf-life is regulated by iron availability;transcription is controlled by activatorsReb1p, Gcr1p, and Rap1p through binding sitesin the 5' non-coding region 0.00360.08866&radic&radic S000003368LSB1YGR136WverifiedProtein containing an N-terminal SH3 domain; binds Las17p,which is a homolog of human Wiskott-AldrichSyndrome protein involved in actin patchassembly and actin polymerization 0.003610.08866&radic&radic S000003995SSL1YLR005WverifiedComponent of the core form of RNA polymerase transcriptionfactor TFIIH, which has both protein kinase andDNA-dependent ATPase/helicase activities and isessential for transcription and nucleotideexcision repair; interacts with Tfb4p 0.003590.08791&radic&radic S000005431NBA1YOL070CverifiedProtein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cellperiphery, cytoplasm, and bud neck; potentialCdc28p substrate 0.003580.08791&radic&radic&radic S000005570IRC23YOR044WuncharacterizedPutative protein of unknown function; green fluorescent protein(GFP)-fusion localizes to the ER; null mutantdisplays increased levels of spontaneous Rad52foci 0.003590.08791&radic&radic&radic S000002903VPS3YDR495CverifiedCytoplasmic protein required for the sorting and processing ofsoluble vacuolar proteins, acidification of thevacuolar lumen, and assembly of the vacuolarH+-ATPase 0.003590.08791&radic&radic&radic S000003083SNF4YGL115WverifiedProtein kinase activator found in a complex containing Snf1pand members of the Sip1p/Sip2p/Gal83p family;activates the Snf1p protein kinase; involved inexpression of glucose-repressed genes,sporulation, and peroxisome biogenesis 0.003580.0878&radic S000002715YDR307WUncharacterizedHypothetical protein 0.003570.08749&radic S000005724BFR1YOR198CverifiedComponent of mRNP complexes associated with polyribosomes;implicated in secretion and nuclearsegregation; multicopy suppressor of BFA(Brefeldin A) sensitivity 0.003570.08749&radic&radic&radic&radic S000003750HXT8YJL214WverifiedProtein of unknown function with similarity to hexosetransporter family members, expression isinduced by low levels of glucose and repressedby high levels of glucose 0.003570.08749&radic&radic&radic S000000514STP22YCL008CverifiedComponent of the ESCRT-I complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; homologous to the mouse and humanTsg101 tumor susceptibility gene; mutantsexhibit a Class E Vps phenotype 0.003560.08739&radic&radic S000005366TOP1YOL006CverifiedTopoisomerase I, nuclear enzyme that relieves torsional strainin DNA by cleaving and re-sealing thephosphodiester backbone; relaxes bothpositively and negatively supercoiled DNA;functions in replication, transcription, andrecombination 0.003560.08739&radic&radic&radic&radic&radic S000002719TFB1YDR311WverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, required for nucleotide excisionrepair, target for transcriptional activators 0.003560.08739&radic&radic S000004239YEF3YLR249WverifiedTranslational elongation factor, stimulates the binding ofaminoacyl-tRNA (AA-tRNA) to ribosomes byreleasing EF-1 alpha from the ribosomalcomplex; contains two ABC cassettes; binds andhydrolyses ATP 0.003560.08739&radic&radic&radic S000001104RPP1YHR062CverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.003560.0872&radic&radic S000005873REV1YOR346WverifiedDeoxycytidyl transferase, forms a complex with the subunits ofDNA polymerase zeta, Rev3p and Rev7p; involvedin repair of abasic sites in damaged DNA 0.003560.0872&radic&radic&radic&radic S000002800SPT3YDR392WverifiedSubunit of the SAGA and SAGA-like transcriptional regulatorycomplexes, interacts with Spt15p to activatetranscription of some RNA polymeraseII-dependent genes, also functions to inhibittranscription at some promoters 0.003560.0872&radic&radic S000003039AFT1YGL071WverifiedTranscription factor involved in iron utilization andhomeostasis; binds the consensus sitePyPuCACCCPu and activates the expression oftarget genes in response to changes in ironavailability 0.003550.08664&radic S000002411RAD57YDR004WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad55p 0.003550.08664&radic S000000545GLK1YCL040WverifiedGlucokinase, catalyzes the phosphorylation of glucose at C6 inthe first irreversible step of glucosemetabolism; one of three glucosephosphorylating enzymes; expression regulatedby non-fermentable carbon sources 0.003540.08644&radic&radic&radic S000004273YLR283WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene 0.003540.08644&radic&radic&radic&radic S000000735GCN4YEL009CverifiedTranscriptional activator of amino acid biosynthetic genes inresponse to amino acid starvation; expressionis tightly regulated at both thetranscriptional and translational levels 0.003530.08608&radic&radic&radic S000003461SMI1YGR229CverifiedProtein involved in the regulation of cell wall synthesis;proposed to be involved in coordinating cellcycle progression with cell wall integrity 0.003530.08608&radic&radic&radic S000001261YHR218WUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.003530.08597&radic&radic S000005976LGE1YPL055CverifiedProtein of unknown function; null mutant forms abnormally largecells 0.003520.08584&radic&radic S000006185YPL264CUncharacterizedHypothetical protein 0.003520.08584&radic S000004708YMR102CUncharacterizedProtein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene 0.003520.08578&radic S000003710KRE9YJL174WverifiedGlycoprotein involved in cell wall beta-glucan assembly; nullmutation leads to severe growth defects,aberrant multibudded morphology, and matingdefects 0.003520.08578&radic S000002386HOYDL227CverifiedSite-specific endonuclease required for gene conversion at theMAT locus (homothallic switching) through thegeneration of a ds DNA break; expressionrestricted to mother cells in late G1 ascontrolled by Swi4p-Swi6p, Swi5p and Ash1p 0.003510.08537&radic S000003890YJR129CUncharacterizedPutative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.003520.08537&radic&radic S000005322ZRG17YNR039CverifiedEndoplasmic reticulum protein of unknown function,transcription is induced under conditions ofzinc deficiency; mutant phenotype suggests arole in uptake of zinc 0.00350.08494 S000000217YBR013CUncharacterizedHypothetical protein 0.00350.08494&radic&radic S000004067FMP25YLR077WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.003490.08441&radic&radic S000000103SLA1YBL007CverifiedCytoskeletal protein binding protein required for assembly ofthe cortical actin cytoskeleton; contains 3 SH3domains; interacts with proteins regulatingactin dynamics and with proteins required forendocytosis 0.003490.08441&radic&radic S000004199PNP1YLR209CverifiedPurine nucleoside phosphorylase, specifically metabolizesinosine and guanosine nucleosides 0.003490.08441&radic&radic&radic S000002838CYM1YDR430CverifiedLysine-specific metalloprotease of the mitochondrialintermembrane space, member of the pitrilysinfamily; degrades proteins and presequencepeptides cleaved from imported proteins;required for normal mitochondrial morphology 0.003490.08441&radic&radic&radic S000004420YLR428CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 0.003480.08441 S000000124YBL028CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis 0.003490.08441&radic&radic&radic S000003712SWI3YJL176CverifiedSubunit of the SWI/SNF chromatin remodeling complex, whichregulates transcription by remodelingchromosomes; required for transcription of manygenes, including ADH1, ADH2, GAL1, HO, INO1 andSUC2 0.003470.08415&radic&radic&radic&radic S000006295YPR091CUncharacterizedHypothetical protein 0.003470.08415&radic&radic S000002703HDA2YDR295CverifiedSubunit of a possibly tetrameric trichostatin A-sensitive classII histone deacetylase complex containing anHda1p homodimer and an Hda2p-Hda3p heterodimer;involved in telomere maintenance 0.003450.08279&radic&radic&radic S000003801GEF1YJR040WverifiedChloride channel localized to late- or post-Golgi vesicles,involved in iron metabolism; highly homologousto voltage-gated chloride channels invertebrates 0.003470.08279&radic&radic S000003319PDC6YGR087CverifiedMinor isoform of pyruvate decarboxylase, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde, regulation is glucose- andethanol-dependent, involved in amino acidcatabolism 0.003440.08279&radic&radic&radic S000002485SHU2YDR078CuncharacterizedProtein of unassigned function involved in mutationsuppression, important for error-free repair ofspontaneous and induced DNA lesions to protectthe genome from mutation; associates withShu1p, Psy3p, and Csm2p 0.003460.08279&radic&radic&radic S000006430MRP10YDL045W-AverifiedMitochondrial ribosomal protein of the small subunit 0.003470.08279&radic S000005497BSC6YOL137WverifiedProtein of unknown function containing 8 putative transmembraneseqments; ORF exhibits genomic organizationcompatible with a translationalreadthrough-dependent mode of expression 0.003460.08279 S000001146GRE3YHR104WverifiedAldose reductase involved in methylglyoxal, d-xylose andarabinose metabolism; stress induced (osmotic,ionic, oxidative, heat shock, starvation andheavy metals); regulated by the HOG pathway 0.003460.08279&radic&radic&radic&radic S000003420BUB1YGR188CverifiedProtein kinase that forms a complex with Mad1p and Bub3p thatis crucial in the checkpoint mechanism requiredto prevent cell cycle progression into anaphasein the presence of spindle damage, associateswith centromere DNA via Skp1p 0.003470.08279&radic S000001081MSC7YHR039CverifiedProtein of unknown function, green fluorescent protein(GFP)-fusion protein localizes to theendoplasmic reticulum; msc7 mutants aredefective in directing meiotic recombinationevents to homologous chromatids 0.003450.08279&radic&radic&radic&radic S000000446YBR242WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene 0.003440.08279&radic&radic&radic&radic S000006317PIS1YPR113WverifiedPhosphatidylinositol synthase, required for biosynthesis ofphosphatidylinositol, which is a precursor forpolyphosphoinositides, sphingolipids, andglycolipid anchors for some of the plasmamembrane proteins 0.003460.08279&radic&radic S000002944STL1YDR536WverifiedGlycerol proton symporter of the plasma membrane, subject toglucose-induced inactivation, strongly buttransiently induced when cells are subjected toosmotic shock 0.003430.08256&radic&radic S000004281YLR290CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR290C is not an essential gene 0.003420.0822&radic&radic&radic&radic&radic S000002178RPN4YDL020CverifiedTranscription factor that stimulates expression of proteasomegenes; Rpn4p levels are in turn regulated bythe 26S proteasome in a negative feedbackcontrol mechanism; RPN4 is transcriptionallyregulated by various stress responses 0.003420.0822&radic S000001609YPK1YKL126WverifiedSerine/threonine protein kinase required for receptor-mediatedendocytosis; involved in sphingolipid-mediatedand cell integrity signaling pathways;localized to the bud neck, cytosol and plasmamembrane; homolog of mammalian kinase SGK 0.003420.08177&radic S000004640MSN2YMR037CverifiedTranscriptional activator related to Msn4p; activated in stressconditions, which results in translocation fromthe cytoplasm to the nucleus; binds DNA atstress response elements of responsive genes,inducing gene expression 0.003410.08177&radic S000002567SSY1YDR160WverifiedComponent of the SPS plasma membrane amino acid sensor system(Ssy1p-Ptr3p-Ssy5p), which senses externalamino acid concentration and transmitsintracellular signals that result in regulationof expression of amino acid permease genes 0.003410.08177&radic&radic S000002284CDC48YDL126CverifiedATPase in ER, nuclear membrane and cytosol with homology tomammalian p97; in a complex with Npl4p andUfd1p participates in retrotranslocation ofubiquitinated proteins from the ER into thecytosol for degradation by the proteasome 0.003410.08177&radic S000001021OCA5YHL029CuncharacterizedCytoplasmic protein required for replication of Brome mosaicvirus in S. cerevisiae, which is a model systemfor studying replication of positive-strand RNAviruses in their natural hosts 0.003410.08177&radic S000003925RTT109YLL002WverifiedProtein with a role in regulation of Ty1 transposition 0.00340.08136&radic&radic&radic&radic S000006335NAT3YPR131CverifiedCatalytic subunit of the NatB N-terminal acetyltransferase,which catalyzes acetylation of theamino-terminal methionine residues of allproteins beginning with Met-Asp or Met-Glu andof some proteins beginning with Met-Asn orMet-Met 0.00340.08136&radic&radic S000004907YMR293CUncharacterizedPutative protein similar to bacterial glutamyl-tRNA amidotransferases; may be involved in aerobic respiration; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR293C is not an essential gene 0.00340.08136&radic&radic&radic&radic S000005979PDR12YPL058CverifiedPlasma membrane weak-acid-inducible ATP-binding cassette (ABC)transporter, required for weak organic acidresistance, strongly induced by sorbate andbenzoate, regulated by War1p, mutants exhibitsorbate hypersensitivity 0.003390.08113&radic&radic S000004045SPT8YLR055CverifiedSubunit of the SAGA transcriptional regulatory complex but notpresent in SAGA-like complex SLIK/SALSA,required for SAGA-mediated inhibition at somepromoters 0.003380.08073&radic&radic S000002794MUS81YDR386WverifiedHelix-hairpin-helix protein, involved in DNA repair andreplication fork stability; functions as anendonuclease in complex with Mms4p; interactswith Rad54p 0.003370.08073&radic S000003176SIP2YGL208WverifiedOne of three beta subunits of the Snf1 serine/threonine proteinkinase complex involved in the response toglucose starvation; null mutants exhibitaccelerated aging; N-myristoylprotein localizedto the cytoplasm and the plasma membrane 0.003380.08073&radic&radic S000000770IES6YEL044WverifiedProtein that associates with the INO80 chromatin remodelingcomplex under low-salt conditions 0.003380.08073&radic&radic&radic S000005670ELG1YOR144CverifiedProtein required for S phase progression and telomerehomeostasis, forms an alternative replicationfactor C complex important for DNA replicationand genome integrity; involved in homologousrecombination-mediated DNA repair 0.003370.08052&radic&radic&radic&radic S000005831ISW2YOR304WverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPasecomponent that, with Itc1p, forms a complexrequired for repression of a-specific genes,INO1, and early meiotic genes during mitoticgrowth 0.003360.08043&radic&radic&radic S000001492MRT4YKL009WverifiedProtein involved in mRNA turnover and ribosome assembly,localizes to the nucleolus 0.003360.08027&radic&radic&radic S000001066MAS2YHR024CverifiedLarger subunit of the mitochondrial processing protease (MPP),essential processing enzyme that cleaves theN-terminal targeting sequences frommitochondrially imported proteins 0.003360.07983&radic&radic&radic S000003193VRG4YGL225WverifiedGolgi GDP-mannose transporter; regulates Golgi function andglycosylation in Golgi 0.003360.07983&radic&radic S000001240RIX1YHR197WverifiedEssential protein involved in the processing of the ITS2 regionof the rRNA locus; required for the maturationand nuclear export of the 60S ribosomal subunit 0.003350.07983&radic&radic&radic S000000112FUS3YBL016WverifiedMitogen-activated protein kinase involved in mating pheromoneresponse; activated by phosphorylation bySte7p; provides specificity during the matingvs. filamentous growth response byphosphorylating transcriptional and cytoplasmictargets 0.003360.07983&radic&radic&radic S000005306SNF12YNR023Wverified73 kDa subunit of the SWI/SNF chromatin remodeling complexinvolved in transcriptional regulation; homologof Rsc6p subunit of the RSC chromatinremodeling complex; deletion mutants aretemperature-sensitive 0.003360.07983&radic&radic&radic S000004913LIP1YMR298WverifiedCeramide synthase subunit; single-span ER membrane proteinassociated with Lag1p and Lac1p and requiredfor ceramide synthase activity, null mutantgrows extremely slowly and is defective inceramide synthesis 0.003340.07959&radic&radic&radic&radic S000002447ENA1YDR040CverifiedP-type ATPase sodium pump, involved in Na+ and Li+ efflux toallow salt tolerance 0.003320.0786&radic&radic S000003446RPS0AYGR214WverifiedProtein component of the small (40S) ribosomal subunit, nearlyidentical to Rps0Bp; required for maturation of18S rRNA along with Rps0Bp; deletion of eitherRPS0 gene reduces growth rate, deletion of bothgenes is lethal 0.003340.0786&radic S000000963SPT2YER161CverifiedProtein involved in negative regulation of transcription;required for RNA polyadenylation; exhibitsregulated interactions with both histones andSWI-SNF components, has similarity to mammalianHMG1 proteins 0.003330.0786&radic S000005509DCP1YOL149WverifiedSubunit of the Dcp1p-Dcp2p decapping enzyme complex, whichremoves the 5' cap structure from mRNAs priorto their degradation; enhances the activity ofcatalytic subunit Dcp2p; regulated by DEAD boxprotein Dhh1p 0.003330.0786&radic&radic&radic S000002910SAM2YDR502CverifiedS-adenosylmethionine synthetase, catalyzes transfer of theadenosyl group of ATP to the sulfur atom ofmethionine; one of two differentially regulatedisozymes (Sam1p and Sam2p) 0.003310.0786&radic&radic S000000979BMH1YER177Wverified14-3-3 protein, major isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.003320.0786 S000004664SAM37YMR060CverifiedComponent of the mitochondrial outer membrane sorting andassembly machinery (SAM) complex; required forthe sorting of some proteins to the outermembrane after import by the TOM complex 0.003330.0786&radic&radic&radic S000002954SOM1YEL059C-AverifiedSubunit of the mitochondrial inner membrane peptidase, which isrequired for maturation of mitochondrialproteins of the intermembrane space; Som1pfacilitates cleavage of a subset of substrates 0.003320.0786&radic S000003897YJR136CUncharacterizedPutative protein of unknown function; may interact with mitochondrial ribosomal protein Rsm23p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.003330.0786&radic&radic S000001541TOA2YKL058WverifiedTFIIA small subunit; involved in transcriptional activation,acts as antirepressor or as coactivator;homologous to smallest subunit of human andDrosophila TFIIA 0.003310.0786&radic&radic S000000533RNQ1YCL028Wverified[PIN(+)] prion, an infectious protein conformation that isgenerally an ordered protein aggregate 0.003330.0786&radic&radic&radic S000001022ECM29YHL030WverifiedMajor component of the proteasome; tethers the proteasome coreparticle to the regulatory particle, andenhances the stability of the proteasome 0.003320.0786&radic&radic S000000798RMD6YEL072WverifiedProtein required for sporulation 0.00330.07829&radic&radic&radic S000000364CDC28YBR160WverifiedCatalytic subunit of the main cell cycle cyclin-dependentkinase (CDK); alternately associates with G1cyclins (CLNs) and G2/M cyclins (CLBs) whichdirect the CDK to specific substrates 0.00330.07829&radic&radic S000005309SEC12YNR026CverifiedGuanine nucleotide exchange factor (GEF); glycosylated integralmembrane protein of the endoplasmic reticulum,important for the initiation of transportvesicle budding from the endoplasmic reticulumthrough activation of the GTPase Sar1p 0.003310.07829&radic S000001324ARC15YIL062CverifiedSubunit of the ARP2/3 complex, which is required for themotility and integrity of cortical actinpatches 0.003310.07829&radic S000003003MIG1YGL035CverifiedTranscription factor involved in glucose repression; C2H2 zincfinger protein similar to mammalian Egr andWilms tumor proteins 0.003310.07829&radic S000002100VMA10YHR039C-AverifiedVacuolar H+ ATPase subunit G of the catalytic (V1) sector,involved in vacuolar acidification 0.00330.07829&radic S000006360TPO3YPR156CverifiedPolyamine transport protein specific for spermine; localizes tothe plasma membrane; member of the majorfacilitator superfamily 0.00330.07819&radic&radic&radic S000000362AMN1YBR158WverifiedProtein required for daughter cell separation, multiple mitoticcheckpoints, and chromosome stability; contains12 degenerate leucine-rich repeat motifs;expression is induced by the Mitotic ExitNetwork (MEN) 0.00330.07819&radic S000000897RAD51YER095WverifiedStrand exchange protein, forms a helical filament with DNA thatsearches for homology; involved in therecombinational repair of double-strand breaksin DNA during vegetative growth and meiosis;homolog of Dmc1p and bacterial RecA protein 0.00330.07819&radic S000000123RPL19BYBL027WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl19Ap and has similarity to ratL19 ribosomal protein; rpl19a and rpl19b singlenull mutations result in slow growth, while thedouble null mutation is lethal 0.00330.07819&radic&radic S000001177YCK1YHR135CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck2p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck2p 0.00330.07819&radic&radic&radic S000001006YLF2YHL014CverifiedProtein of unknown function, has weak similarity to E. coliGTP-binding protein gtp1; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 0.003290.07761&radic&radic&radic&radic S000000539LSB5YCL034WverifiedProtein of unknown function; binds Las17p, which is a homologof human Wiskott-Aldrich Syndrome proteininvolved in actin patch assembly and actinpolymerization 0.003280.07761&radic&radic&radic S000001745SPC34YKR037CverifiedEssential subunit of the Dam1 complex (aka DASH complex),couples kinetochores to the force produced byMT depolymerization thereby aiding inchromosome segregation; also localized tonuclear side of spindle pole body 0.00330.07761&radic&radic&radic&radic&radic S000001676SDS22YKL193CverifiedConserved nuclear regulatory subunit of Glc7p type 1 proteinserine-threonine phosphatase (PP1), functionspositively with Glc7p to promotedephosphorylation of nuclear substratesrequired for chromosome transmission duringmitosis 0.003290.07761&radic&radic&radic S000001305CBR1YIL043CverifiedMicrosomal cytochrome b reductase, not essential for viability;also detected in mitochondria; mutation inconserved NADH binding domain of the humanortholog results in type I methemoglobinemia 0.003290.07761&radic&radic&radic S000006034YPL113CUncharacterizedPutative dehydrogenase 0.003280.07761&radic&radic&radic&radic S000005187SLA2YNL243WverifiedTransmembrane actin-binding protein involved in membranecytoskeleton assembly and cell polarization;adaptor protein that links actin to clathrinand endocytosis; present in the actin corticalpatch of the emerging bud tip; dimer in vivo 0.003280.07761 S000003011DST1YGL043WverifiedGeneral transcription elongation factor TFIIS, enables RNApolymerase II to read through blocks toelongation by stimulating cleavage of nascenttranscripts stalled at transcription arrestsites 0.003290.07761&radic&radic&radic S000003200YGL231CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum 0.00330.07761&radic&radic&radic S000001458MUC1YIR019CverifiedGPI-anchored cell surface glycoprotein required for diploidpseudohyphal formation and haploid invasivegrowth, transcriptionally regulated by the MAPKpathway (via Ste12p and Tec1p) and the cAMPpathway (via Flo8p) 0.003290.07761&radic S000006099CBC2YPL178WverifiedSmall subunit of the heterodimeric cap binding complex thatalso contains Sto1p, component of thespliceosomal commitment complex; interacts withNpl3p, possibly to package mRNA for export fromthe nucleus; contains an RNA-binding motif 0.003270.07689&radic&radic&radic S000001571YKL088WUncharacterizedPredicted phosphopantothenoylcysteine decarboxylase, may be involved in coenzyme A biosynthesis; interacts with Sis2p and Vhs3p 0.003270.07689&radic&radic&radic&radic S000004637YMR034CUncharacterizedPutative protein of unknown function with similarity to transporters; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene 0.003270.07689&radic&radic S000006210ICL2YPR006Cverified2-methylisocitrate lyase of the mitochondrial matrix, functionsin the methylcitrate cycle to catalyze theconversion of 2-methylisocitrate to succinateand pyruvate; ICL2 transcription is repressedby glucose and induced by ethanol 0.003280.07689&radic S000003218ZIP2YGL249WverifiedMeiosis-specific protein involved in normal synaptonemalcomplex formation and pairing betweenhomologous chromosomes during meiosis 0.003270.07689&radic S000005354YNR071CUncharacterizedHypothetical protein 0.003270.07689&radic&radic S000002970ERP6YGL002WverifiedProtein with similarity to Emp24p and Erv25p, member of the p24family involved in ER to Golgi transport; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 0.003270.07689&radic&radic&radic S000000881YER079WUncharacterizedHypothetical protein 0.003270.07689&radic&radic S000006038IDI1YPL117CverifiedIsopentenyl diphosphate:dimethylallyl diphosphate isomerase(IPP isomerase), catalyzes an essentialactivation step in the isoprenoid biosyntheticpathway; required for viability 0.003260.07684&radic S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.003250.07626&radic S000005028END3YNL084CverifiedEH domain-containing protein involved in endocytosis, actincytoskeletal organization and cell wallmorphogenesis; forms a complex with Sla1p andPan1p 0.003250.07626&radic&radic S000002606RKM2YDR198CverifiedRibosomal protein lysine methyltransferase, responsible forepsilon-trimethylation of the lysine residue atposition 10 of Rpl12Ap and Rpl12Bp 0.003260.07626&radic&radic&radic S000001572MIF2YKL089WverifiedKinetochore protein with homology to human CENP-C, required forstructural integrity of the spindle duringanaphase spindle elongation, interacts withhistones H2A, H2B, and H4, phosphorylated byIpl1p 0.003250.07626&radic&radic&radic&radic S000001346SDS3YIL084CverifiedComponent of the Rpd3p/Sin3p deacetylase complex required forits structural integrity and catalyticactivity, involved in transcriptional silencingand required for sporulation; cells defectivein SDS3 display pleiotropic phenotypes 0.003250.07626&radic&radic&radic S000004073EMP70YLR083CverifiedProtein whose 24kDa cleavage product is found inendosome-enriched membrane fractions, predictedto be a transmembrane protein 0.003260.07626&radic&radic&radic&radic&radic S000000784PCM1YEL058WverifiedEssential N-acetylglucosamine-phosphate mutase, ahexosephosphate mutase involved in thebiosynthesis of chitin 0.003260.07626&radic&radic&radic S000003973COF1YLL050CverifiedCofilin, promotes actin filament depolarization in apH-dependent manner; binds both actin monomersand filaments and severs filaments , thought tobe regulated by phosphorylation at SER4,ubiquitous and essential in eukaryotes 0.003240.07626&radic&radic S000005892YOR365CUncharacterizedHypothetical protein 0.003250.07626&radic&radic S000004133YLR143WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; YLR143W is not an essential gene 0.003250.07626&radic&radic&radic&radic S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif 0.003240.07614&radic&radic&radic&radic S000001072SLT2YHR030CverifiedSerine/threonine MAP kinase involved in regulating themaintenance of cell wall integrity andprogression through the cell cycle; regulatedby the PKC1-mediated signaling pathway 0.003240.07597&radic&radic S000002921GRX2YDR513WverifiedCytoplasmic glutaredoxin, thioltransferase,glutathione-dependent disulfide oxidoreductaseinvolved in maintaining redox state of targetproteins, also exhibits glutathione peroxidaseactivity, expression induced in response tostress 0.003240.07597&radic&radic&radic S000001329YIL067CUncharacterizedHypothetical protein 0.003240.07597&radic S000006320YPR116WUncharacterizedHypothetical protein 0.003230.07547&radic&radic&radic S000002570CWC15YDR163WverifiedNon-essential protein involved in pre-mRNA splicing, componentof a complex containing Cef1p; has similarityto S. pombe Cwf15p 0.003230.07547&radic&radic&radic&radic&radic S000004385ATP10YLR393WverifiedMitochondrial inner membrane protein required for assembly ofthe F0 sector of mitochondrial F1F0 ATPsynthase, interacts genetically with ATP6 0.003220.07547&radic S000004470SPT5YML010WverifiedProtein that forms a complex with Spt4p and mediates bothactivation and inhibition of transcriptionelongation; Spt4p-Spt5p complex also plays arole in pre-mRNA processing 0.003220.07547&radic&radic&radic&radic S000002591PLP1YDR183WverifiedProtein with a possible role in folding of beta-tubulin; hassimilarity to phosducins, which are GTPaseinhibitors 0.003220.07526&radic&radic&radic S000004124PDC5YLR134WverifiedMinor isoform of pyruvate decarboxylase, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde, regulation is glucose- andethanol-dependent, repressed by thiamine,involved in amino acid catabolism 0.003220.07526&radic S000005451SPO21YOL091WverifiedComponent of the meiotic outer plaque of the spindle pole body,involved in modifying the meiotic outer plaquethat is required prior to prospore membraneformation 0.003220.07526&radic&radic&radic S000002590CDC1YDR182WverifiedPutative membrane protein of unknown function involved in Mn2+homeostasis; mutants display actin and generalgrowth defects, heterogeneous cell cyclearrests, and pleiotropic defects in cell cycleprogression and organelle distribution 0.003220.07526&radic&radic&radic S000002814PDR15YDR406WverifiedATP binding cassette (ABC) transporter of the plasma membrane;general stress response factor implicated incellular detoxification; target of Pdr1p, Pdr3pand Pdr8p transcription regulators; promotercontains a PDR responsive element 0.003210.07526&radic&radic&radic S000005947SKS1YPL026CverifiedPutative serine/threonine protein kinase; involved in theadaptation to low concentrations of glucoseindependent of the SNF3 regulated pathway 0.003220.07526&radic S000006305SNT309YPR101WverifiedComponent of NineTeen complex (NTC) containing Prp19p involvedin mRNA splicing, interacts physically andgenetically with Prp19p 0.003210.07428&radic&radic&radic S000002569NBP2YDR162CverifiedProtein involved in the HOG (high osmolarity glycerol) pathway,negatively regulates Hog1p by recruitment ofphosphatase Ptc1p the Pbs2p-Hog1p complex,found in the nucleus and cytoplasm, contains anSH3 domain that binds Pbs2p 0.003190.07428&radic&radic&radic&radic&radic S000003633PHS1YJL097WuncharacterizedProtein of unknown function; homolog of mammalian PTPLA;involved in sphingolipid biosynthesis, proteintrafficking; required for cell viability 0.00320.07428&radic&radic S000003677YAK1YJL141CverifiedSerine-threonine protein kinase that is part of aglucose-sensing system involved in growthcontrol in response to glucose availability;translocates from the cytoplasm to the nucleusand phosphorylates Pop2p in response to aglucose signal 0.003210.07428 S000000516SGF29YCL010CverifiedSaGa associated Factor 29kDa; Probable 29kKDa Subunit of SAGAhistone acetyltransferase complex 0.003190.07428&radic S000001596RAD27YKL113Cverified5' to 3' exonuclease, 5' flap endonuclease, required forOkazaki fragment processing and maturation aswell as for long-patch base-excision repair;member of the S. pombe RAD2/FEN1 family 0.00320.07428&radic S000003993YLR003CUncharacterizedPutative protein of unknown function that may participate in DNA replication; green fluorescent protein (GFP)-fusion protein is localized to the nucleus; YLR003C is not an essential gene 0.003190.07428&radic&radic&radic&radic S000005713TUF1YOR187WverifiedMitochondrial translation elongation factor Tu; comprises bothGTPase and guanine nucleotide exchange factoractivities, while these activities are found inseparate proteins in S. pombe and humans 0.003190.07428&radic&radic&radic S000005365RPB11YOL005CverifiedRNA polymerase II subunit B12.5; part of central core; similarto Rpc19p and bacterial alpha subunit 0.00320.07428&radic S000004219CDC42YLR229CverifiedSmall rho-like GTPase, essential for establishment andmaintenance of cell polarity; mutants havedefects in the organization of actin andseptins 0.00320.07428 S000000786PRB1YEL060CverifiedVacuolar proteinase B (yscB), a serine protease of thesubtilisin family; involved in proteindegradation in the vacuole and required forfull protein degradation during sporulation 0.003210.07428&radic S000001445PAN1YIR006CverifiedPart of actin cytoskeleton-regulatory complexPan1p-Sla1p-End3p, associates with actinpatches on the cell cortex; promotesprotein-protein interactions essential forendocytosis; previously thought to be a subunitof poly(A) ribonuclease 0.003210.07428&radic S000005177BNI4YNL233WverifiedTargeting subunit for Glc7p protein phosphatase, localized tothe bud neck, required for localization ofchitin synthase III to the bud neck viainteraction with the chitin synthase IIIregulatory subunit Skt5p 0.003190.07428&radic S000005760RPL33BYOR234CverifiedRibosomal protein L37 of the large (60S) ribosomal subunit,nearly identical to Rpl33Ap and has similarityto rat L35a; rpl33b null mutant exhibits normalgrowth while rpl33a rpl33b double null mutantis inviable 0.003190.07428&radic&radic&radic&radic S000001870EPL1YFL024CverifiedComponent of NuA4, which is an essential histone H4/H2Aacetyltransferase complex; homologous toDrosophila Enhancer of Polycomb 0.003180.07415&radic&radic&radic S000004537TCB3YML072CverifiedLipid-binding protein, localized to the bud via specific mRNAtransport; non-tagged protein localizes tomitochondria and GFP-fusion protein localizesto the cell periphery; C-termini of Tcb1p,Tcb2p and Tcb3p interact 0.003180.07414 S000003950ISA1YLL027WverifiedMitochondrial matrix protein involved in biogenesis of theiron-sulfur (Fe/S) cluster of Fe/S proteins,isa1 deletion causes loss of mitochondrial DNAand respiratory deficiency; depletion reducesgrowth on nonfermentable carbon sources 0.003170.07357&radic&radic&radic S000004862GAD1YMR250WverifiedGlutamate decarboxylase, converts glutamate intogamma-aminobutyric acid (GABA) during glutamatecatabolism; involved in response to oxidativestress 0.003170.07357&radic&radic&radic&radic S000006076KIP2YPL155CverifiedKinesin-related motor protein involved in mitotic spindlepositioning, stabilizes microtubules bytargeting Bik1p to the plus end; Kip2p levelsare controlled during the cell cycle 0.003170.07357&radic&radic S000003872YJR111CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria 0.003170.07357&radic&radic S000002885SNF1YDR477WverifiedAMP-activated serine/threonine protein kinase found in acomplex containing Snf4p and members of theSip1p/Sip2p/Gal83p family; required fortranscription of glucose-repressed genes,thermotolerance, sporulation, and peroxisomebiogenesis 0.003170.07357 S000003480SOL4YGR248Wverified6-phosphogluconolactonase with similarity to Sol3p 0.003170.07357&radic&radic S000003413TIM13YGR181WverifiedMitochondrial intermembrane space protein, forms a complex withTIm8p that mediates import and insertion of asubset of polytopic inner membrane proteins;may prevent aggregation of incoming proteins ina chaperone-like manner 0.003170.07357&radic&radic&radic&radic S000005952PHO85YPL031CverifiedCyclin-dependent kinase, with ten cyclin partners; involved inenvironmental stress response; inphosphate-rich conditions, Pho85p-Pho80pcomplex phosphorylates Pho4p which in turnrepresses PHO5 0.003160.07357&radic&radic S000001618APL2YKL135CverifiedBeta-adaptin, large subunit of the clathrin-associated protein(AP-1) complex; binds clathrin; involved inclathrin-dependent Golgi protein sorting 0.003160.07357&radic&radic&radic S000000633PHO87YCR037CverifiedLow-affinity inorganic phosphate (Pi) transporter, involved inactivation of PHO pathway; expression isindependent of Pi concentration and Pho4pactivity; contains 12 membrane-spanningsegments 0.003140.07235&radic&radic&radic S000002892VPS52YDR484WverifiedComponent of the GARP (Golgi-associated retrograde protein)complex, Vps51p-Vps52p-Vps53p-Vps54p, which isrequired for the recycling of proteins fromendosomes to the late Golgi; involved inlocalization of actin and chitin 0.003150.07235&radic S000003692SSY5YJL156CverifiedProbable serine protease of the SPS plasma membrane amino acidsensor system (Ssy1p-Ptr3p-Ssy5p), which sensesexternal amino acid concentration and transmitsintracellular signals that regulate expressionof amino acid permease genes 0.003150.07235&radic S000004215YLR225CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene 0.003140.07235&radic&radic S000005659EFT1YOR133WverifiedElongation factor 2 (EF-2), also encoded by EFT2; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 0.003140.07235&radic&radic&radic S000005098YCK2YNL154CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck1p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck1p 0.003140.07235&radic&radic&radic&radic&radic S000000128HEK2YBL032WverifiedRNA binding protein with similarity to hnRNP-K that localizesto the cytoplasm and to subtelomeric DNA;required for the proper localization of ASH1mRNA; involved in the regulation of telomereposition effect and telomere length 0.003130.07235&radic S000001160ORC6YHR118CverifiedSubunit of the origin recognition complex, which directs DNAreplication by binding to replication originsand is also involved in transcriptionalsilencing; may be phosphorylated by Cdc28p 0.003140.07235&radic&radic&radic&radic S000004647IOC4YMR044WverifiedMember of a complex (Isw1b) with Isw1p and Ioc2p that exhibitsnucleosome-stimulated ATPase activity and actswithin coding regions to coordinatetranscription elongation with termination andprocessing, contains a PWWP motif 0.003130.07235&radic&radic S000003769TDH2YJR009CverifiedGlyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involvedin glycolysis and gluconeogenesis; tetramerthat catalyzes the reaction ofglyceraldehyde-3-phosphate to 1,3bis-phosphoglycerate; detected in the cytoplasmand cell-wall 0.003150.07235&radic S000006274MED1YPR070WverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation 0.003140.07235&radic&radic S000002711RSC3YDR303CverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; essential gene required forregulation of ribosomal protein genes and thecell wall/stress response; highly similar toRsc30p 0.003130.07235&radic&radic S000000945DDI1YER143WverifiedDNA damage-inducible v-SNARE binding protein, contains aubiquitin-associated (UBA) domain, may act as anegative regulator of constitutive exocytosis,may play a role in S-phase checkpoint control 0.003130.07235&radic S000000959COG3YER157WverifiedEssential component of the conserved oligomeric Golgi complex(Cog1p through Cog8p), a cytosolic tetheringcomplex that functions in protein traffickingto mediate fusion of transport vesicles toGolgi compartments 0.003140.07235 S000000824SRB4YER022WverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation 0.003160.07235&radic&radic S000006272HOS1YPR068CverifiedPutative class I histone deacetylase (HDAC) with sequencesimilarity to Hda1p, Rpd3p, Hos2p, and Hos3p;deletion results in increased histoneacetylation at rDNA repeats; interacts with theTup1p-Ssn6p corepressor complex 0.003130.07235&radic&radic&radic S000001390MET18YIL128WverifiedDNA repair and TFIIH regulator, required for both nucleotideexcision repair (NER) and RNA polymerase II(RNAP II) transcription; involved in telomeremaintenance 0.003160.07235&radic S000003616SCP160YJL080CverifiedEssential RNA-binding G protein effector of mating responsepathway, predominantly associated with nuclearenvelope and ER, interacts in mRNA-dependentmanner with translating ribosomes via multipleKH domains, similar to vertebrate vigilins 0.003140.07235 S000000214HHT1YBR010WverifiedOne of two identical histone H3 proteins (see also HHT2); corehistone required for chromatin assembly,involved in heterochromatin-mediated telomericand HM silencing; regulated by acetylation,methylation, and mitotic phosphorylation 0.003140.07235&radic S000004887RCE1YMR274CverifiedType II CAAX prenyl protease involved in the proteolysis andmaturation of Ras and the a-factor matingpheromone 0.003160.07235&radic&radic&radic S000004140STM1YLR150WverifiedProtein that binds G4 quadruplex and purine motif triplexnucleic acid; acts with Cdc13p to maintaintelomere structure; interacts with ribosomesand subtelomeric Y' DNA; multicopy suppressorof tom1 and pop2 mutations 0.003120.07126&radic S000001750UTH1YKR042WverifiedMitochondrial outer membrane and cell wall localized SUN familymember required for mitochondrial autophagy;involved in the oxidative stress response, lifespan during starvation, mitochondrialbiogenesis, and cell death 0.003120.07126&radic&radic