Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0031124 "mRNA 3'-end processing"

GO branch: BP
Number of currently annotated genes: 21

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest Kumar:nucleusHuh:nucleusTAP:162Lum:DaunorubicinTAP:111Lum:ClomipraminePS50158IPR012677PF05182IPR000504 S000003388PTI1YGR156WverifiedPta1p Interacting protein &radic0.390430.92364&radic&radic&radic&radic&radic&radic&radic&radic S000004105CFT2YLR115WverifiedSubunit of the mRNA cleavage and polyadenlylation factor (CPF);required for pre-mRNA cleavage, polyadenylationand poly(A) site recognition, 43% similaritywith the mammalian CPSF-100 protein. &radic0.37470.91704&radic&radic&radic&radic&radic&radic S000004267YSH1YLR277CverifiedPutative endonuclease, subunit of the mRNA cleavage andpolyadenylation specificity complex requiredfor 3' processing of mRNAs &radic0.349610.88164&radic&radic&radic&radic&radic S000005261PFS2YNL317WverifiedIntegral subunit of the pre-mRNA cleavage and polyadenylationfactor (CPF) complex; plays an essential rolein mRNA 3'-end formation by bridging differentprocessing factors and thereby promoting theassembly of the processing complex &radic0.34730.88164&radic&radic&radic&radic&radic S000000041PTA1YAL043CverifiedSubunit of holo-CPF, a multiprotein complex and functionalhomolog of mammalian CPSF, required for thecleavage and polyadenylation of mRNA and snoRNA3' ends; involved in pre-tRNA processing; bindsto the phosphorylated CTD of RNAPII &radic0.345460.88054&radic&radic&radic&radic&radic&radic S000005166SSU72YNL222WverifiedTranscription/RNA-processing factor that associates with TFIIBand cleavage/polyadenylation factor Pta1p;exhibits phosphatase activity on serine-5 ofthe RNA polymerase II C-terminal domain;affects start site selection in vivo 0.342530.87296&radic&radic&radic&radic S000002603REF2YDR195WverifiedRNA-binding protein involved in the cleavage step of mRNA3'-end formation prior to polyadenylation, andin snoRNA maturation; part of holo-CPFsubcomplex APT, which associates with 3'-endsof snoRNA- and mRNA-encoding genes 0.320090.85806&radic&radic&radic&radic S000006311YTH1YPR107CverifiedEssential RNA-binding component of cleavage and polyadenylationfactor, contains five zinc fingers; requiredfor pre-mRNA 3'-end processing andpolyadenylation &radic0.309860.85251&radic&radic&radic&radic&radic S000002636PCF11YDR228CverifiedmRNA 3' end processing factor, essential component of cleavageand polyadenylation factor IA (CF IA), involvedin pre-mRNA 3' end processing and intranscription termination; binds C-terminaldomain of largest subunit of RNA pol II(Rpo21p) &radic0.295150.83778&radic&radic&radic&radic S000000935GLC7YER133WverifiedCatalytic subunit of type 1 serine/threonine proteinphosphatase, involved in many processesincluding glycogen metabolism, sporulation, andmitosis; interacts with multiple regulatorysubunits; predominantly isolated with Sds22p 0.292970.83452&radic&radic&radic S000004665RNA14YMR061WverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; bridges interaction between Rna15p andHrp1p in the CF I complex &radic0.267610.81873&radic&radic&radic S000003853FIP1YJR093CverifiedSubunit of cleavage polyadenylation factor (CPF), interactsdirectly with poly(A) polymerase (Pap1p) toregulate its activity &radic0.249140.80088&radic&radic&radic&radic S000002709CFT1YDR301WverifiedRNA-binding subunit of the mRNA cleavage and polyadenylationfactor; involved in poly(A) site recognitionand required for both pre-mRNA cleavage andpolyadenylation, 51% sequence similarity withmammalian AAUAA-binding subunit of CPSF &radic0.226310.78571&radic&radic&radic S000003012RNA15YGL044CverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; interacts with the A-rich polyadenylationsignal in complex with Rna14p and Hrp1p &radic0.216790.77246&radic&radic&radic&radic S000003062PAN2YGL094CverifiedEssential subunit of the Pan2p-Pan3p poly(A)-ribonucleasecomplex, which acts to control poly(A) taillength and regulate the stoichiometry andactivity of postreplication repair complexes &radic0.198130.75505 S000005705SYC1YOR179CverifiedSubunit of the APT subcomplex of cleavage and polyadenylationfactor, may have a role in 3' end formation ofboth polyadenylated and non-polyadenylated RNAs 0.180140.74247&radic&radic S000005776CLP1YOR250CverifiedSubunit of cleavage factor I (CFI), involved in both theendonucleolyitc cleavage and polyadenylationsteps of mRNA 3'-end maturation &radic0.17740.7399&radic&radic&radic S000001508PAN3YKL025CverifiedEssential subunit of the Pan2p-Pan3p poly(A)-ribonucleasecomplex, which acts to control poly(A) taillength and regulate the stoichiometry andactivity of postreplication repair complexes &radic0.155680.70833&radic&radic S000000551YCL046WDubiousHypothetical protein 0.148010.69722&radic&radic S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif &radic0.135110.6794&radic&radic&radic&radic&radic S000001855ACT1YFL039CverifiedActin, structural protein involved in cell polarization,endocytosis, and other cytoskeletal functions 0.129850.67171&radic S000000221KAP104YBR017CverifiedTransportin, cytosolic karyopherin beta 2 involved in deliveryof heterogeneous nuclear ribonucleoproteins tothe nucleoplasm, binds rg-nuclear localizationsignals on Nab2p and Hrp1p, plays a role incell-cycle progression 0.114830.6436&radic S000003017TIF4632YGL049CverifiedTranslation initiation factor eIF4G, subunit of the mRNAcap-binding protein complex (eIF4F) that alsocontains eIF4E (Cdc33p); associates with thepoly(A)-binding protein Pab1p, also interactswith eIF4A (Tif1p); homologous to Tif4631p 0.065860.5476&radic&radic S000001297CKA1YIL035CverifiedAlpha catalytic subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA2, CKB1 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 0.058660.52609&radic&radic&radic S000004198SEC13YLR208WverifiedComponent of both the Nup84 nuclear pore sub-complex and of theCOPII complex (Sar1p, Sec13p, Sec16p, Sec23p,Sec24p, Sec31p, Sfb2p, and Sfb3p) which isimportant for the formation of ER to Golgitransport vesicles 0.054340.51032&radic&radic&radic S000004975HHF2YNL030WverifiedOne of two identical histone H4 proteins (see also HHF1); corehistone required for chromatin assembly andchromosome function; contributes to telomericsilencing; N-terminal domain involved inmaintaining genomic integrity 0.053160.50553&radic&radic&radic S000005871TYE7YOR344CverifiedSerine-rich protein that contains a basic-helix-loop-helix(bHLH) DNA binding motif; binds E-boxes ofglycolytic genes and contributes to theiractivation; may function as a transcriptionalactivator in Ty1-mediated gene expression 0.049380.48759&radic&radic&radic S000003472PFK1YGR240CverifiedAlpha subunit of heterooctameric phosphofructokinase involvedin glycolysis, indispensable for anaerobicgrowth, activated by fructose-2,6-bisphosphateand AMP, mutation inhibits glucose induction ofcell cycle-related genes 0.047810.48156&radic&radic S000002633HTA1YDR225WverifiedOne of two nearly identical (see also HTA2) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.047220.4788&radic&radic S000004492YML030WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YML030W is not an essential gene 0.046420.47431&radic&radic&radic S000000171SSA3YBL075CverifiedATPase involved in protein folding and the response to stress;plays a role in SRP-dependent cotranslationalprotein-membrane targeting and translocation;member of the heat shock protein 70 (HSP70)family; localized to the cytoplasm 0.04540.47048&radic&radic S000003080TAF6YGL112CverifiedSubunit (60 kDa) of TFIID and SAGA complexes, involved intranscription initiation of RNA polymerase IIand in chromatin modification, similar tohistone H4 0.042450.45633&radic&radic S000000967PAB1YER165WverifiedPoly(A) binding protein, part of the 3'-end RNA-processingcomplex, mediates interactions between the 5'cap structure and the 3' mRNA poly(A) tail,involved in control of poly(A) tail length,interacts with translation factor eIF-4G 0.04150.45134&radic&radic&radic S000000309VID24YBR105CverifiedPeripheral membrane protein located at Vid (vacuole import anddegradation) vesicles; regulatesfructose-1,6-bisphosphatase (FBPase) targetingto the vacuole; involved inproteasome-dependent catabolite degradation ofFBPase 0.040490.44607&radic&radic S000005475PAP2YOL115WverifiedCatalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), anuclear poly (A) polymerase complex involved inRNA quality control; catalyzes polyadenylationof unmodified tRNAs, and snoRNA and rRNAprecursors; disputed role as a DNA polymerase 0.039610.44135&radic S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.037270.42807&radic&radic&radic&radic S000004211RSA3YLR221CverifiedProtein with a likely role in ribosomal maturation, requiredfor accumulation of wild-type levels of large(60S) ribosomal subunits; binds to the helicaseDbp6p in pre-60S ribosomal particles in thenucleolus 0.037180.42788&radic S000002354SEC31YDL195WverifiedEssential phosphoprotein component (p150) of the COPII coat ofsecretory pathway vesicles, in complex withSec13p; required for ER-derived transportvesicle formation 0.036840.42549&radic S000001027VMR1YHL035CverifiedProtein of unknown function that may interact with ribosomes,based on co-purification experiments; member ofthe ATP-binding cassette (ABC) family;potential Cdc28p substrate; detected inpurified mitochondria in high-throughputstudies 0.036890.42549&radic&radic S000003566VPS53YJL029CverifiedComponent of the GARP (Golgi-associated retrograde protein)complex, Vps51p-Vps52p-Vps53p-Vps54p, which isrequired for the recycling of proteins fromendosomes to the late Golgi; required forvacuolar protein sorting 0.036690.42473&radic S000001217ENO2YHR174WverifiedEnolase II, a phosphopyruvate hydratase that catalyzes theconversion of 2-phosphoglycerate tophosphoenolpyruvate during glycolysis and thereverse reaction during gluconeogenesis;expression is induced in response to glucose 0.034070.41072&radic S000001710PAP1YKR002WverifiedPoly(A) polymerase, one of three factors required for mRNA3'-end polyadenylation, forms multiproteincomplex with polyadenylation factor I (PF I),also required for mRNA nuclear export; may alsopolyadenylate rRNAs &radic0.033230.4054&radic&radic&radic S000003450CRM1YGR218WverifiedMajor karyopherin, involved in export of proteins, RNAs, andribosomal subunits from the nucleus 0.033110.40447&radic&radic&radic S000005483HRP1YOL123WverifiedSubunit of cleavage factor I, a five-subunit complex requiredfor the cleavage and polyadenylation ofpre-mRNA 3' ends; RRM-containing heteronuclearRNA binding protein and hnRNPA/B family memberthat binds to poly (A) signal sequences &radic0.025650.35909&radic&radic&radic&radic S000002299RPO21YDL140CverifiedRNA polymerase II largest subunit B220, part of central core;phosphorylation of C-terminal heptapeptiderepeat domain regulates association withtranscription and splicing factors; similar tobacterial beta-prime 0.023990.34716&radic&radic S000002218TSR1YDL060WverifiedProtein required for processing of 20S pre-rRNA in thecytoplasm, associates with pre-40S ribosomalparticles 0.022390.33263&radic S000002789YRA1YDR381WverifiedNuclear protein that binds to RNA and to Mex67p, required forexport of poly(A)+ mRNA from the nucleus;member of the REF (RNA and export factorbinding proteins) family; another familymember, Yra2p, can substitute for Yra1pfunction 0.020880.31875&radic&radic&radic&radic&radic&radic S000002430SES1YDR023WverifiedCytosolic seryl-tRNA synthetase, class II aminoacyl-tRNAsynthetase that aminoacylates tRNA(Ser),displays tRNA-dependent amino acid recognitionwhich enhances discrimination of the serinesubstrate, interacts with peroxin Pex21p 0.020650.31549 S000004895AEP2YMR282CverifiedMitochondrial protein, likely involved in translation of themitochondrial OLI1 mRNA; exhibits geneticinteraction with the OLI1 mRNA 5'-untranslatedleader 0.020670.31549&radic S000004642SUB1YMR039CverifiedTranscriptional coactivator, facilitates elongation byinfluencing enzymes that modify RNAP II, actsin a peroxide resistance pathway involvingRad2p; suppressor of TFIIB mutations 0.017530.27824&radic&radic&radic S000000064SWD1YAR003WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres; WD40beta propeller superfamily member withsimilarity to mammalian Rbbp7 0.016090.26045&radic S000002758ATP22YDR350CverifiedMitochondrial inner membrane protein required for assembly ofthe F0 sector of mitochondrial F1F0 ATPsynthase, which is a large, evolutionarilyconserved enzyme complex required for ATPsynthesis 0.015860.25784&radic&radic S000002453BAP3YDR046CverifiedAmino acid permease involved in the uptake of cysteine,leucine, isoleucine and valine 0.015480.25155&radic&radic S000003279TFC4YGR047CverifiedOne of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauA domain of TFIIIC that binds BoxA DNApromoter sites of tRNA and similar genes; hasTPR motifs; human homolog is TFIIIC-102 0.01490.24286&radic&radic&radic S000000912KAP123YER110CverifiedKaryopherin beta, mediates nuclear import of ribosomal proteinsprior to assembly into ribosomes and import ofhistones H3 and H4; localizes to the nuclearpore, nucleus, and cytoplasm; exhibits geneticinteractions with RAI1 0.014420.23664&radic&radic S000004513RSE1YML049CverifiedProtein involved in pre-mRNA splicing; component of thepre-spliceosome; associates with U2 snRNA;involved in ER to Golgi transport 0.014280.23456 S000002349UFD2YDL190CverifiedUbiquitin chain assembly factor (E4) that cooperates with aubiquitin-activating enzyme (E1), aubiquitin-conjugating enzyme (E2), and aubiquitin protein ligase (E3) to conjugateubiquitin to substrates; also functions as an 0.013840.22778&radic S000004868GFD1YMR255WverifiedCoiled-coiled protein of unknown function, identified as ahigh-copy suppressor of a dbp5 mutation 0.013330.22114&radic S000005952PHO85YPL031CverifiedCyclin-dependent kinase, with ten cyclin partners; involved inenvironmental stress response; inphosphate-rich conditions, Pho85p-Pho80pcomplex phosphorylates Pho4p which in turnrepresses PHO5 0.011810.19904&radic&radic&radic&radic S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.011620.19621&radic&radic S000002798UBA2YDR390CverifiedNuclear protein that acts as a heterodimer with Aos1p toactivate Smt3p (SUMO) before its conjugation toproteins (sumoylation), which may play a rolein protein targeting; essential for viability 0.011560.19613&radic&radic&radic&radic S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus 0.010940.18742&radic&radic S000000323MUD1YBR119WverifiedU1 snRNP A protein, homolog of human U1-A; involved in nuclearmRNA splicing 0.010650.18356&radic&radic&radic&radic S000005433THP1YOL072WverifiedNuclear pore-associated protein, forms a complex with Sac3pthat is involved in transcription and in mRNAexport from the nucleus; contains a PAM domainimplicated in protein-protein binding 0.010350.1791&radic&radic S000001803MLP1YKR095WverifiedMyosin-like protein associated with the nuclear envelope,connects the nuclear pore complex with thenuclear interior; involved with Tel1p intelomere length control; involved with Pml1pand Pml39p in nuclear retention of unsplicedmRNAs 0.010220.17755&radic S000001163LSM12YHR121WverifiedProtein of unknown function that interacts with Pbp1p and Pbp4pand associates with ribosomes; contains anRNA-binding LSM domain and an AD domain; mayplay a role in RNA processing 0.010210.17739&radic&radic&radic S000005898GPB1YOR371CverifiedProposed beta subunit of the heterotrimeric G protein;interacts with the receptor Grp1p, affectssignaling by altering the level ofphosphorylation of PKS substrates; regulatespseudohyphal growth through cAMP levels;homolog of Gpb2p 0.009770.17097&radic S000002697RTT103YDR289CverifiedProtein that interacts with exonuclease Rat1p and Rai1p andplays a role in transcription termination byRNA polymerase II, has an RPR domain(carboxy-terminal domain interacting domain);also involved in regulation of Ty1transposition 0.009560.16737&radic&radic&radic S000004097REX3YLR107WverifiedRNA exonuclease; required for maturation of the RNA componentof RNase MRP; functions redundantly with Rnh70pand Rex2p in processing of U5 snRNA and RNase PRNA; member of RNase D family of exonucleases 0.009490.16649&radic&radic&radic S000003427SKI6YGR195Wverified3'-to-5' phosphorolytic exoribonuclease that is a subunit ofthe exosome; required for 3' processing of the5.8S rRNA; involved in 3' to 5' mRNAdegradation and translation inhibition ofnon-poly(A) mRNAs 0.009220.16162&radic&radic&radic S000000307SIF2YBR103WverifiedWD40 repeat-containing subunit of the Set3C histone deacetylasecomplex, which represses early/middlesporulation genes; antagonizes telomericsilencing; binds specifically to the Sir4pN-terminus 0.009190.16125&radic S000005440REX4YOL080CverifiedPutative RNA exonuclease possibly involved in pre-rRNAprocessing and ribosome assembly 0.00920.16125&radic&radic&radic&radic S000003508RNH70YGR276Cverified3' exoribonuclease, required for 5S and tRNA-Arg3 maturation 0.009190.16113 S000003959PRP19YLL036CverifiedSplicing factor associated with the spliceosome; contains aU-box, a motif found in a class of ubiquitinligases 0.008960.15684&radic&radic S000001411MLP2YIL149CverifiedMyosin-like protein associated with the nuclear envelope,connects the nuclear pore complex with thenuclear interior; involved in the Tel1p pathwaythat controls telomere length 0.008750.15414&radic S000004049REX2YLR059CverifiedRNA exonuclease, required for U4 snRNA maturation; functionsredundantly with Rnh70p in 5.8S rRNAmaturation, and with Rnh70p and Rex3p inprocessing of U5 snRNA and RNase P RNA; memberof RNase D family of exonucleases 0.008720.15372&radic&radic&radic S000004961PUB1YNL016WverifiedPoly(A)+ RNA-binding protein, abundant mRNP-component proteinthat binds mRNA and is required for stabilityof a number of mRNAs; not reported to associatewith polyribosomes 0.008520.15068&radic&radic&radic S000003280UFD1YGR048WverifiedProtein that interacts with Cdc48p and Npl4p, involved inrecognition of polyubiquitinated proteins andtheir presentation to the 26S proteasome fordegradation; involved in transporting proteinsfrom the ER to the cytosol 0.007970.14263&radic S000005195NRD1YNL251CverifiedRNA-binding protein that interacts with the C-terminal domainof the RNA polymerase II large subunit(Rpo21p), required for transcriptiontermination and 3' end maturation ofnonpolyadenylated RNAs 0.007970.14263&radic&radic&radic&radic&radic&radic S000005929CHL1YPL008WverifiedConserved nuclear protein required to establishsister-chromatid pairing during S-phase,probable DNA helicase with similarity to humanBACH1, which associates with tumor suppressorBRCA1; associates with acetyltransferase Ctf7p 0.007620.13654&radic&radic&radic S000002971CDH1YGL003CverifiedCell-cycle regulated activator of the anaphase-promotingcomplex/cyclosome (APC/C), which directsubiquitination of mitotic cyclins resulting inexit from mitosis; targets the APC/C tospecific substrates including CDC20, ASE1 andCIN8 0.007460.1343&radic&radic S000002334AIR2YDL175CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p; may regulatemethylation of Npl3p, which modulates Npl3pfunction in mRNA processing and export; hassimilarity to Air1p 0.007410.13348&radic&radic&radic S000005243TRF5YNL299WverifiedPoly (A) polymerase involved in nuclear RNA quality controlbased on: homology with Trf4p, geneticinteractions with TRF4 mutants, physicalinteraction with Mtr4p (TRAMP subunit), and bydirect assay; disputed role as a sigma DNApolymerase 0.007390.13276 S000002495SLU7YDR088CverifiedRNA splicing factor, required for ATP-independent portion of2nd catalytic step of spliceosomal RNAsplicing; interacts with Prp18p; contains zincknuckle domain 0.007340.13168&radic&radic&radic&radic&radic S000004685NAM7YMR080CverifiedATP-dependent RNA helicase of the SFI superfamily, required fornonsense mediated mRNA decay and for efficienttranslation termination at nonsense codons;involved in telomere maintenance 0.006950.1244 S000003570HCA4YJL033WverifiedPutative nucleolar DEAD box RNA helicase; high-copy numbersuppression of a U14 snoRNA processing mutantsuggests an involvement in 18S rRNA synthesis 0.006770.12235&radic S000000379SWD3YBR175WverifiedEssential subunit of the COMPASS (Set1C) complex, whichmethylates histone H3 on lysine 4 and isrequired in transcriptional silencing neartelomeres; WD40 beta propeller superfamilymember and ortholog of mammalian WDR5 0.006760.12206&radic&radic S000005199GIS2YNL255CverifiedProtein with seven cysteine-rich CCHC zinc-finger motifs,similar to human CNBP, proposed to be involvedin the RAS/cAMP signaling pathway 0.006760.12206&radic&radic S000001622CTK1YKL139WverifiedCatalytic (alpha) subunit of C-terminal domain kinase I(CTDK-I), which phosphorylates the C-terminalrepeated domain of the RNA polymerase II largesubunit (Rpo21p) to affect both transcriptionand pre-mRNA 3' end processing 0.006720.12179&radic&radic S000006161HSP82YPL240CverifiedCytoplasmic chaperone (Hsp90 family) required for pheromonesignaling and negative regulation of Hsf1p;docks with the mitochondrial import receptorTom70p for preprotein delivery; interacts withco-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p 0.006680.1208&radic&radic S000001501SWD2YKL018WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lys 4 and is involved intelomeric silencing; subunit of CPF (cleavageand polyadenylation factor), a complex involvedin RNAP II transcription termination &radic0.006640.12053&radic S000004470SPT5YML010WverifiedProtein that forms a complex with Spt4p and mediates bothactivation and inhibition of transcriptionelongation; Spt4p-Spt5p complex also plays arole in pre-mRNA processing 0.00660.11988&radic&radic S000004140STM1YLR150WverifiedProtein that binds G4 quadruplex and purine motif triplexnucleic acid; acts with Cdc13p to maintaintelomere structure; interacts with ribosomesand subtelomeric Y' DNA; multicopy suppressorof tom1 and pop2 mutations 0.006490.1182&radic&radic S000001504MAK11YKL021CverifiedProtein essential for cell growth and replication of M dsRNAvirus; contains four beta-transducin repeats 0.00650.1182&radic&radic&radic S000004421CRN1YLR429WverifiedCoronin, cortical actin cytoskeletal component that associateswith the Arp2p/Arp3p complex to regulate itsactivity 0.006450.11711 S000000638TAF2YCR042CverifiedTFIID subunit (150 kDa), involved in RNA polymerase IItranscription initiation 0.006360.11548&radic S000005133SRP1YNL189WverifiedKaryopherin alpha homolog, forms a dimer with karyopherin betaKap95p to mediate import of nuclear proteins,binds the nuclear localization signal of thesubstrate during import; may also play a rolein regulation of protein degradation 0.006350.1151&radic&radic&radic S000005823TIM18YOR297CverifiedComponent of the mitochondrial Tim54p-Tim22p complex involvedin insertion of polytopic proteins into theinner membrane; may function to stabilize thecomplex 0.00630.11434&radic S000006141RPL1AYPL220WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl1Bpand has similarity to E. coli L1 and rat L10aribosomal proteins; rpl1a rpl1b double nullmutation is lethal 0.006270.11394&radic&radic S000002432RPS11AYDR025WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps11Bp and has similarity to E.coli S17 and rat S11 ribosomal proteins 0.006250.11353&radic S000001450STS1YIR011CverifiedProtein that interacts with the karyopherin Srp1p; may have arole with Srp1p in ubiquitin-mediated proteindegradation 0.006260.11353&radic&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p 0.006120.1112&radic&radic&radic S000003432ELP2YGR200CverifiedSubunit of Elongator complex, which is required formodification of wobble nucleosides in tRNA;target of Kluyveromyces lactis zymocin 0.006070.10991&radic&radic S000002506BMH2YDR099Wverified14-3-3 protein, minor isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.005990.10875&radic&radic S000004106MSL5YLR116WverifiedComponent of the commitment complex, which defines the firststep in the splicing pathway; essential proteinthat interacts with Mud2p and Prp40p, forming abridge between the intron ends; also involvedin nuclear retention of pre-mRNA 0.005990.10875&radic&radic&radic&radic S000004699UTP15YMR093WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.005940.10776&radic&radic&radic S000002259DUN1YDL101CverifiedCell-cycle checkpoint serine-threonine kinase required for DNAdamage-induced transcription of certain targetgenes, phosphorylation of Rad55p and Sml1p, andtransient G2/M arrest after DNA damage; alsoregulates postreplicative DNA repair 0.005850.10617&radic&radic S000004332RPP0YLR340WverifiedConserved ribosomal protein P0 similar to rat P0, human P0, andE. coli L10e; shown to be phosphorylated onserine 302 0.005770.10427&radic S000004034PDC1YLR044CverifiedMajor of three pyruvate decarboxylase isozymes, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde; subject to glucose-, ethanol-,and autoregulation; involved in amino acidcatabolism 0.005760.10427&radic&radic&radic S000005401NOP12YOL041CverifiedNucleolar protein, required for pre-25S rRNA processing;contains an RNA recognition motif (RRM) and hassimilarity to Nop13p, Nsr1p, and putativeorthologs in Drosophila and S. pombe 0.005730.10357&radic&radic&radic&radic S000005738STE4YOR212WverifiedG protein beta subunit, forms a dimer with Ste18p to activatethe mating signaling pathway, forms aheterotrimer with Gpa1p and Ste18p to dampensignaling; may recruit Rho1p to the polarizedgrowth site during mating; contains WD40repeats 0.005730.10357&radic S000005524BDS1YOL164WverifiedBacterially-derived sulfatase required for use of alkyl- andaryl-sulfates as sulfur sources 0.005730.10357 S000000973RAD3YER171Wverified5' to 3' DNA helicase, involved in nucleotide excision repairand transcription; subunit of RNA polymerase IItranscription initiation factor TFIIH; subunitof Nucleotide Excision Repair Factor 3 (NEF3);homolog of human XPD protein 0.005690.10271&radic S000003506TAF1YGR274CverifiedTFIID subunit (145 kDa), involved in RNA polymerase IItranscription initiation, has histoneacetyltransferase activity, involved inpromoter binding and G1/S progression 0.005650.10188&radic&radic&radic&radic S000006132NIP7YPL211WverifiedNucleolar protein required for 60S ribosome subunit biogenesis,constituent of 66S pre-ribosomal particles;physically interacts with Nop8p and the exosomesubunit Rrp43p 0.005640.10144&radic&radic&radic S000005566GLO4YOR040WverifiedMitochondrial glyoxalase II, catalyzes the hydrolysis ofS-D-lactoylglutathione into glutathione andD-lactate 0.005620.1005&radic&radic S000002972RPN14YGL004CuncharacterizedPutative non-ATPase subunit of the 19S regulatory particle ofthe 26S proteasome; localized to the cytoplasm 0.005510.0991&radic&radic S000002982PUF4YGL014WverifiedMember of the PUF protein family, which is defined by thepresence of Pumilio homology domains thatconfer RNA binding activity; preferentiallybinds mRNAs encoding nucleolar ribosomalRNA-processing factors 0.005340.09551&radic&radic S000003410PBP1YGR178CverifiedProtein interacting with poly(A)-binding protein Pab1p; likelyinvolved in controlling the extent of mRNApolyadenylation; forms a complex with Mkt1pthat may regulate HO translation; interactswith Lsm12p in a copurification assay &radic0.005340.09533&radic&radic&radic&radic S000001448MSL1YIR009WverifiedU2B component of U2 snRNP, involved in splicing, binds the U2snRNA stem-loop IV in vitro; does not containthe conserved C-terminal RNA binding domainfound in other family members 0.005330.09523&radic&radic&radic S000001071YHI9YHR029CverifiedProtein of unknown function that is a member of the PhzFsuperfamily, although unlike its bacterialhomolog, is most likely not involved inphenazine production; possibly involved in amembrane regulation metabolic pathway 0.005320.09473&radic&radic S000001025RPL8AYHL033CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Bp and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 0.005290.0944 S000002680GLO2YDR272WverifiedCytoplasmic glyoxalase II, catalyzes the hydrolysis ofS-D-lactoylglutathione into glutathione andD-lactate 0.005230.09308&radic&radic&radic S000001022ECM29YHL030WverifiedMajor component of the proteasome; tethers the proteasome coreparticle to the regulatory particle, andenhances the stability of the proteasome 0.005220.09295&radic&radic&radic&radic S000002558CTH1YDR151CverifiedMember of the CCCH zinc finger family; has similarity tomammalian Tis11 protein, which activatestranscription and also has a role in mRNAdegradation; may function with Tis11p in ironhomeostasis 0.005210.09255&radic S000003391NSR1YGR159CverifiedNucleolar protein that binds nuclear localization sequences,required for pre-rRNA processing and ribosomebiogenesis 0.005180.09233&radic&radic&radic&radic&radic S000005733RET1YOR207CverifiedSecond-largest subunit of RNA polymerase III, which isresponsible for the transcription of tRNA and5S RNA genes, and other low molecular weightRNAs 0.005160.0919&radic&radic&radic S000006059SPP1YPL138CverifiedSubunit of COMPASS (Set1C), a complex which methylates histoneH3 on lysine 4 and is required in telomerictranscriptional silencing; PHD finger domainprotein similar to human CGBP, an unmethylatedCpG binding protein 0.005120.09138&radic&radic&radic S000002600NUP42YDR192CverifiedSubunit of the nuclear pore complex (NPC) that localizesexclusively to the cytoplasmic side; involvedin RNA export, most likely at a terminal step;interacts with Gle1p 0.00510.0906 S000005617TMA46YOR091WverifiedProtein of unknown function that associates with ribosomes;interacts with GTPase Rbg1p 0.005090.0906 S000001587GFA1YKL104CverifiedGlutamine-fructose-6-phosphate amidotransferase, catalyzes theformation of glucosamine-6-P and glutamate fromfructose-6-P and glutamine in the first step ofchitin biosynthesis 0.005030.08945 S000000979BMH1YER177Wverified14-3-3 protein, major isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.005010.08907 S000001292SSM4YIL030CverifiedUbiquitin-protein ligase of the ER/nuclear envelope, requiredfor degradation of Alpha2p and other proteinscontaining a Deg1 degradation signal; ssm4mutation suppresses mRNA instability caused byan rna14 mutation 0.004930.08755 S000004440RPL6BYLR448WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to Rpl6Bp and to rat L6 ribosomalprotein; binds to 5.8S rRNA 0.004920.08739 S000005589RPL3YOR063WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to E. coli L3 and rat L3 ribosomalproteins; involved in the replication andmaintenance of killer double stranded RNA virus 0.004910.08701&radic&radic S000003934SOF1YLL011WverifiedEssential subunit of the U3 (box C+D) snRNP complex requiredfor 2' O-methylation of pre-RNA; has similarityto the beta subunit of trimeric G-proteins andthe splicing factor Prp4p 0.004830.0855&radic&radic S000003202SEC15YGL233WverifiedEssential 113kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which mediates polarized targeting ofvesicles to active sites of exocytosis; Sec15passociates with Sec4p and vesicles 0.004810.08512&radic&radic S000001604YKL121WUncharacterizedPutative protein of unknown function 0.004750.08405&radic&radic S000004393DUS3YLR401CuncharacterizedDihydrouridine synthase, member of a widespread family ofconserved proteins including Smm1p, Dus1p, andDus4p; contains a consensus oleate responseelement (ORE) in its promoter region 0.004770.08405&radic S000004401UTP21YLR409CuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.004710.08347&radic&radic S000004212UTP13YLR222CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.004690.08283&radic&radic&radic S000004005BRE2YLR015WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres;involved in telomere maintenance; similar totrithorax-group protein ASH2L 0.004670.08252&radic&radic&radic S000004315CWC24YLR323CverifiedEssential protein, component of a complex containing Cef1p; hassimilarity to S. pombe Cwf24p 0.004650.08234&radic&radic&radic S000000104HIR1YBL008WverifiedNon-essential transcriptional corepressor involved in the cellcycle-regulated transcription of histone H2A,H2B, H3 and H4 genes; contributes to nucleosomeformation, heterochromatic gene silencing, andformation of functional kinetochores 0.004640.08226&radic S000004134ACF2YLR144CverifiedIntracellular beta-1,3-endoglucanase, expression is inducedduring sporulation; may have a role in corticalactin cytoskeleton assembly 0.004630.0819&radic&radic&radic&radic S000004668AEP1YMR064WverifiedProtein required for expression of the mitochondrial OLI1 geneencoding subunit 9 of F1-F0 ATP synthase 0.004570.08055 S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.004530.08024 S000005097GIM3YNL153CverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 0.004470.07894&radic S000001323SNP1YIL061CverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; may interact with poly(A)polymerase to regulate polyadenylation; homologof human U1 70K protein 0.004450.0785&radic&radic&radic&radic S000001697YRA2YKL214CverifiedMember of the REF (RNA and export factor binding proteins)family; when overexpressed, can substitute forthe function of Yra1p in export of poly(A)+mRNA from the nucleus 0.004430.0782&radic&radic&radic&radic S000002549PEX7YDR142CverifiedPeroxisomal signal receptor for the N-terminal nonapeptidesignal (PTS2) of peroxisomal matrix proteins;WD repeat protein; defects in human homologcause lethal rhizomelic chondrodysplasiapunctata (RCDP) 0.004390.07716&radic S000002596CCT6YDR188WverifiedSubunit of the cytosolic chaperonin Cct ring complex, relatedto Tcp1p, essential protein that is requiredfor the assembly of actin and tubulins in vivo;contains an ATP-binding motif 0.004390.07716&radic&radic S000003969RNP1YLL046CverifiedRibonucleoprotein that contains two RNA recognition motifs(RRM) 0.004370.07665&radic&radic&radic S000002707BFR2YDR299WverifiedEssential protein possibly involved in secretion; multicopysuppressor of sensitivity to Brefeldin A 0.004350.07665&radic&radic&radic S000003484GCN5YGR252WverifiedHistone acetyltransferase, acetylates N-terminal lysines onhistones H2B and H3; catalytic subunit of theADA and SAGA histone acetyltransferasecomplexes; founding member of the Gcn5p-relatedN-acetyltransferase superfamily 0.004320.07597&radic&radic S000000884UTP7YER082CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.004250.07465&radic&radic&radic S000005366TOP1YOL006CverifiedTopoisomerase I, nuclear enzyme that relieves torsional strainin DNA by cleaving and re-sealing thephosphodiester backbone; relaxes bothpositively and negatively supercoiled DNA;functions in replication, transcription, andrecombination 0.004250.07465&radic&radic&radic S000001259IMD2YHR216WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, expression is induced bymycophenolic acid resulting in resistance tothe drug, expression is repressed by nutrientlimitation 0.004260.07465 S000006074RAD53YPL153CverifiedProtein kinase, required for cell-cycle arrest in response toDNA damage; activated by transautophosphorylation when interacting withhyperphosphorylated Rad9p; also interacts withARS1 and plays a role in initiation of DNAreplication 0.004230.07393&radic&radic&radic S000004334FKS1YLR342WverifiedCatalytic subunit of 1,3-beta-D-glucan synthase, functionallyredundant with alternate catalytic subunitGsc2p; binds to regulatory subunit Rho1p;involved in cell wall synthesis andmaintenance; localizes to sites of cell wallremodeling 0.004220.07393 S000003968RPL8BYLL045CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Ap and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 0.004230.07393&radic S000002469LCB2YDR062WverifiedComponent of serine palmitoyltransferase, responsible alongwith Lcb1p for the first committed step insphingolipid synthesis, which is thecondensation of serine with palmitoyl-CoA toform 3-ketosphinganine 0.004230.07393&radic S000004650NUP116YMR047CverifiedSubunit of the nuclear pore complex (NPC) that is localized toboth sides of the pore; contains a repetitiveGLFG motif that interacts with mRNA exportfactor Mex67p and with karyopherin Kap95p;homologous to Nup100p 0.00420.07346&radic&radic S000005054NOP15YNL110CverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis; localizes to bothnucleolus and cytoplasm 0.004190.0733&radic&radic&radic&radic S000000729GIM4YEL003WverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 0.004180.0733&radic&radic S000003557BBC1YJL020CverifiedProtein possibly involved in assembly of actin patches;interacts with an actin assembly factor Las17pand with the SH3 domains of Type I myosinsMyo3p and Myo5p; localized predominantly tocortical actin patches 0.004170.07314 S000003322UTP22YGR090WuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.004170.07295&radic&radic&radic&radic&radic S000005604BUD21YOR078WverifiedComponent of small ribosomal subunit (SSU) processosome thatcontains U3 snoRNA; originally isolated asbud-site selection mutant that displays arandom budding pattern 0.004160.07262&radic&radic&radic&radic S000000029GIP4YAL031CverifiedCytoplasmic Glc7-interacting protein whose overexpressionrelocalizes Glc7p from the nucleus and preventschromosome segregation; potential Cdc28psubstrate 0.004140.07236&radic&radic S000003163GCN1YGL195WverifiedPositive regulator of the Gcn2p kinase activity, forms acomplex with Gcn20p; proposed to stimulateGcn2p activation by an uncharged tRNA 0.004130.07191&radic S000000168RPS8AYBL072CverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps8Bp and has similarity to ratS8 ribosomal protein 0.004110.07191&radic&radic S000004424IMD3YLR432WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.00410.07147 S000001696DOA1YKL213CverifiedWD repeat protein required for ubiquitin-mediated proteindegradation, forms complex with Cdc48p, plays arole in controlling cellular ubiquitinconcentration; also promotes efficient NHEJ inpostdiauxic/stationary phase 0.004080.07102&radic&radic S000001444IST3YIR005WverifiedComponent of the U2 snRNP, required for the first catalyticstep of splicing and for spliceosomal assembly;interacts with Rds3p and is required forMer1p-activated splicing 0.004080.07102&radic&radic&radic S000004249HSP60YLR259CverifiedTetradecameric mitochondrial chaperonin required forATP-dependent folding of precursor polypeptidesand complex assembly; prevents aggregation andmediates protein refolding after heat shock;role in mtDNA transmission; similarity to groEL 0.004050.07023&radic S000000027MYO4YAL029CverifiedOne of two type V myosin motors (along with MYO2) involved inactin-based transport of cargos; required formRNA transport, including ASH1 mRNA, andfacilitating the growth and movement of ERtubules into the growing bud along with She3p 0.004040.07023&radic S000004187SIK1YLR197WverifiedEssential evolutionarily-conserved nucleolar protein componentof the box C/D snoRNP complexes that direct2'-O-methylation of pre-rRNA during itsmaturation; overexpression causes spindleorientation defects 0.004030.07007&radic&radic&radic&radic S000001356LYS12YIL094CverifiedHomo-isocitrate dehydrogenase, an NAD-linked mitochondrialenzyme required for the fourth step in thebiosynthesis of lysine, in whichhomo-isocitrate is oxidatively decarboxylatedto alpha-ketoadipate 0.004030.07007 S000004794RGM1YMR182CverifiedPutative transcriptional repressor with proline-rich zincfingers; overproduction impairs cell growth 0.003980.069&radic&radic&radic&radic S000005846HSH49YOR319WverifiedU2-snRNP associated splicing factor with similarity to themammalian splicing factor SAP49; proposed tofunction as a U2-snRNP assembly factor alongwith Hsh155p and binding partner Cus1p;contains two RNA recognition motifs (RRM) 0.0040.069&radic&radic&radic&radic&radic&radic S000004170SAM1YLR180WverifiedS-adenosylmethionine synthetase, catalyzes transfer of theadenosyl group of ATP to the sulfur atom ofmethionine; one of two differentially regulatedisozymes (Sam1p and Sam2p) 0.003960.06833&radic S000000939YER137CUncharacterizedHypothetical protein 0.003930.06794 S000003838MIR1YJR077CverifiedMitochondrial phosphate carrier, imports inorganic phosphateinto mitochondria; functionally redundant withPic2p but more abundant than Pic2 under normalconditions 0.00390.06735 S000006002RPS9AYPL081WverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps9Bp and has similarity to E.coli S4 and rat S9 ribosomal proteins 0.003890.06704&radic S000001557MUD2YKL074CverifiedProtein involved in early pre-mRNA splicing; component of thepre-mRNA-U1 snRNP complex, the commitmentcomplex; interacts with Msl5p/BBP splicingfactor and Sub2p; similar to metazoan splicingfactor U2AF65 0.003890.06704&radic&radic&radic&radic&radic&radic S000005119NOP13YNL175CverifiedProtein of unknown function, localizes to the nucleolus andnucleoplasm; contains an RNA recognition motif(RRM) and has similarity to Nop12p, which isrequired for processing of pre-18S rRNA 0.003880.06684&radic&radic&radic&radic&radic&radic S000002620TCP1YDR212WverifiedAlpha subunit of chaperonin-containing T-complex, whichmediates protein folding in the cytosol;involved in maintenance of actin cytoskeleton;homolog to Drosophila melanogaster and mousetailless complex polypeptide 0.003880.06684&radic&radic S000006099CBC2YPL178WverifiedSmall subunit of the heterodimeric cap binding complex thatalso contains Sto1p, component of thespliceosomal commitment complex; interacts withNpl3p, possibly to package mRNA for export fromthe nucleus; contains an RNA-binding motif 0.003860.06597&radic&radic&radic&radic&radic&radic S000002796RVS167YDR388WverifiedActin-associated protein, subunit of a complex(Rvs161p-Rvs167p) involved in regulation ofactin cytoskeleton, endocytosis, and viabilityfollowing starvation or osmotic stress; homologof mammalian amphiphysin 0.003840.06597&radic S000001062YHR020WUncharacterizedPutative protein of unknown function, has similarity to proline-tRNA ligase; YHR020W is an essential gene 0.003820.06528&radic&radic S000000019CCR4YAL021CverifiedComponent of the CCR4-NOT transcriptional complex, which isinvolved in regulation of gene expression;component of the major cytoplasmic deadenylase,which is involved in mRNA poly(A) tailshortening 0.003820.06528&radic&radic S000001331RPS24BYIL069CverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps24Ap and has similarity to ratS24 ribosomal protein 0.003810.06498 S000000106YBL010CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles 0.003790.06486&radic&radic S000000402TAF5YBR198CverifiedSubunit (90 kDa) of TFIID and SAGA complexes, involved in RNApolymerase II transcription initiation and inchromatin modification 0.003770.06451&radic&radic&radic&radic S000002772CDC40YDR364CverifiedPre-mRNA splicing factor, important for catalytic step II ofpre-mRNA splicing and plays a role in cellcycle progression; required for DNA synthesisduring mitosis and meiosis; has WD repeats 0.003760.06405&radic&radic&radic S000001495PRP40YKL012WverifiedU1 snRNP protein involved in splicing, interacts with thebranchpoint-binding protein during theformation of the second commitment complex 0.003750.06396&radic S000005230CUS2YNL286WverifiedProtein that binds to U2 snRNA and Prp11p, may be involved inU2 snRNA folding; contains two RNA recognitionmotifs (RRMs) 0.003740.06362&radic&radic&radic S000000748GEA2YEL022WverifiedGuanine nucleotide exchange factor for ADP ribosylation factors(ARFs), involved in vesicular transport betweenthe Golgi and ER, Golgi organization, and actincytoskeleton organization; similar to but notfunctionally redundant with Gea1p 0.003740.06362 S000006111NAB3YPL190CverifiedSingle stranded DNA binding protein; acidic ribonucleoprotein;required for termination of non-poly(A)transcripts and efficient splicing; interactswith Nrd1p 0.003720.06303&radic&radic&radic&radic S000001239UTP9YHR196WverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.003710.06303&radic&radic S000003098CEG1YGL130WverifiedAlpha (guanylyltransferase) subunit of the mRNA capping enzyme,a heterodimer (the other subunit is CET1, anRNA 5'-triphophatase) involved in adding the 5'cap to mRNA; the mammalian enzyme is a singlebifunctional polypeptide 0.003710.06303&radic S000005975LEE1YPL054WverifiedZinc-finger protein of unknown function 0.00370.06295&radic S000001551NUP100YKL068WverifiedSubunit of the nuclear pore complex (NPC) that is localized toboth sides of the pore; contains a repetitiveGLFG motif that interacts with mRNA exportfactor Mex67p and with karyopherin Kap95p;homologous to Nup116p 0.00370.06295&radic&radic S000000776RML2YEL050CverifiedMitochondrial ribosomal protein of the large subunit, hassimilarity to E. coli L2 ribosomal protein;fat21 mutant allele causes inability to utilizeoleate and may interfere with activity of theAdr1p transcription factor 0.003690.06274&radic&radic S000001123LRP1YHR081WverifiedSubstrate-specific nuclear cofactor for exosome activity in theprocessing of stable RNAs; required fortelomere length maintenance; homolog ofmammalian nuclear matrix protein C1D involvedin regulation of DNA repair and recombination 0.003680.06252&radic S000001156BZZ1YHR114WverifiedSH3 domain protein implicated in the regulation of actinpolymerization, able to recruit actinpolymerization machinery through its SH3domains, colocalizes with cortical actinpatches and Las17p, interacts with type Imyosins 0.003660.06215&radic&radic&radic S000004681PDS5YMR076CverifiedProtein required for establishment and maintenance of sisterchromatid condensation and cohesion,colocalizes with cohesin on chromosomes in aninterdependent manner, may function as aprotein-protein interaction scaffold 0.003630.06157&radic S000004165CBF5YLR175WverifiedPseudouridine synthase catalytic subunit of box H/ACA smallnucleolar ribonucleoprotein particles(snoRNPs), acts on both large and small rRNAsand on snRNA U2 0.003620.06137&radic&radic&radic&radic S000004614CLU1YMR012WverifiedeIF3 component of unknown function; deletion causes defects inmitochondrial organization but not in growth ortranslation initiation, can rescue cytokinesisand mitochondrial organization defects of theDictyostelium cluA- mutant 0.003620.06137&radic&radic S000005257YNL313CUncharacterizedProtein required for cell viability 0.003590.06082&radic S000002368CWC2YDL209CverifiedProtein involved in pre-mRNA splicing, component of a complexcontaining Cef1p; interacts with Prp19p;contains an RNA recognition motif; hassimilarity to S. pombe Cwf2p 0.003590.06082&radic&radic&radic S000004126TIS11YLR136CverifiedmRNA-binding protein expressed during iron starvation; binds toa sequence element in the 3'-untranslatedregions of specific mRNAs to mediate theirdegradation; involved in iron homeostasis 0.003590.05968&radic&radic S000004754TIF34YMR146CverifiedSubunit of the core complex of translation initiation factor3(eIF3), which is essential for translation 0.003540.05968&radic S000004294MET17YLR303WverifiedO-acetyl homoserine-O-acetyl serine sulfhydrylase, required forsulfur amino acid synthesis 0.003550.05968 S000002877SDC1YDR469WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres; hassimilarity to C. elegans Dpy-30 0.003540.05968&radic S000000532FUS1YCL027WverifiedMembrane protein localized to the shmoo tip, required for cellfusion; expression regulated by matingpheromone; proposed to coordinate signaling,fusion, and polarization events required forfusion; potential Cdc28p substrate 0.003570.05968&radic&radic S000000438ARC40YBR234CverifiedEssential subunit of the ARP2/3 complex, which is required forthe motility and integrity of cortical actinpatches 0.003580.05968 S000004559GIM5YML094WverifiedSubunit of the heterohexameric cochaperone prefoldin complexwhich binds specifically to cytosolicchaperonin and transfers target proteins to it 0.003530.05954 S000002910SAM2YDR502CverifiedS-adenosylmethionine synthetase, catalyzes transfer of theadenosyl group of ATP to the sulfur atom ofmethionine; one of two differentially regulatedisozymes (Sam1p and Sam2p) 0.003530.05954 S000001440SGN1YIR001CverifiedCytoplasmic RNA-binding protein, contains an RNA recognitionmotif (RRM); may have a role in mRNAtranslation, as suggested by geneticinteractions with genes encoding proteinsinvolved in translational initiation 0.003520.05954&radic&radic&radic S000002372NOP6YDL213CuncharacterizedProtein with similarity to hydrophilins, which are involved inthe adaptive response to hyperosmoticconditions; computational analysis oflarge-scale protein-protein interaction datasuggests a possible role in rRNA processing 0.003520.05925&radic&radic&radic S000005619YOR093CUncharacterizedHypothetical protein 0.003510.05925&radic&radic S000000172ILS1YBL076CverifiedCytoplasmic isoleucine-tRNA synthetase, target of theG1-specific inhibitor reveromycin A 0.003520.05925&radic&radic S000004883RRN9YMR270CverifiedProtein involved in promoting high level transcription of rDNA,subunit of UAF (upstream activation factor) forRNA polymerase I 0.003510.05918&radic&radic&radic S000006072PRP46YPL151CverifiedSplicing factor that is found in the Cef1p subcomplex of thespliceosome 0.003490.05888&radic&radic&radic S000002383WHI4YDL224CverifiedPutative RNA binding protein and partially redundant Whi3phomolog that regulates the cell sizerequirement for passage through Start andcommitment to cell division 0.003470.05852&radic&radic&radic S000005964NOP4YPL043WverifiedNucleolar protein, essential for processing and maturation of27S pre-rRNA and large ribosomal subunitbiogenesis; constituent of 66S pre-ribosomalparticles; contains four RNA recognition motifs(RRMs) 0.003470.05852&radic&radic&radic&radic S000004873TIF11YMR260CverifiedTranslation initiation factor eIF1A, essential protein thatforms a complex with Sui1p (eIF1) and the 40Sribosomal subunit and scans for the startcodon; C-terminus associates with Fun12p(eIF5B); N terminus interacts with eIF2 andeIF3 0.003440.05808&radic&radic S000001128NAM8YHR086WverifiedRNA binding protein, component of the U1 snRNP protein; mutantsare defective in meiotic recombination and information of viable spores, involved in theformation of DSBs through meiosis-specificsplicing of MER2 pre-mRNA 0.003450.05808&radic&radic&radic&radic S000003419HGH1YGR187CverifiedProtein of unknown function with similarity to human HMG1 andHMG2; localizes to the cytoplasm 0.003450.05808 S000003783LSM8YJR022WverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)that is part of spliceosomal U6 snRNP and isalso implicated in processing of pre-tRNA,pre-snoRNA, and pre-rRNA 0.003420.05753&radic&radic S000000517GBP2YCL011CverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toHrb1p and Npl3p; also binds single-strandedtelomeric repeat sequence in vitro 0.00340.05728&radic&radic&radic&radic&radic S000001919PES4YFR023WverifiedPoly(A) binding protein, suppressor of DNA polymerase epsilonmutation, similar to Mip6p 0.003390.05723&radic&radic&radic&radic S000005837NOP58YOR310CverifiedProtein involved in pre-rRNA processing, 18S rRNA synthesis,and snoRNA synthesis; component of the smallsubunit processome complex, which is requiredfor processing of pre-18S rRNA 0.003380.05705&radic&radic&radic S000000462SHG1YBR258CverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres 0.003380.05705&radic S000002307NOP14YDL148CverifiedNucleolar protein, forms a complex with Noc4p that mediatesmaturation and nuclear export of 40S ribosomalsubunits; also present in the small subunitprocessome complex, which is required forprocessing of pre-18S rRNA 0.003380.05705&radic S000004911LCB1YMR296CverifiedComponent of serine palmitoyltransferase, responsible alongwith Lcb2p for the first committed step insphingolipid synthesis, which is thecondensation of serine with palmitoyl-CoA toform 3-ketosphinganine 0.003370.05673&radic S000006284TEF1YPR080WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF2; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.003370.05673 S000004072SRL2YLR082CverifiedProtein of unknown function; overexpression suppresses thelethality caused by a rad53 null mutation 0.003350.0565&radic&radic&radic S000002667YAP6YDR259CverifiedPutative basic leucine zipper (bZIP) transcription factor;overexpression increases sodium and lithiumtolerance 0.003350.0565&radic&radic&radic S000005798YTM1YOR272WverifiedConstituent of 66S pre-ribosomal particles, required formaturation of the large ribosomal subunit 0.003340.05637&radic&radic S000001308MET30YIL046WverifiedF-box protein containing five copies of the WD40 motif,controls cell cycle function, sulfurmetabolism, and methionine biosynthesis as partof the ubiquitin ligase complex; interacts withand regulates Met4p, localizes within thenucleus 0.003330.05637&radic&radic&radic S000002591PLP1YDR183WverifiedProtein with a possible role in folding of beta-tubulin; hassimilarity to phosducins, which are GTPaseinhibitors 0.003310.05602&radic&radic S000000030PRP45YAL032CverifiedProtein required for pre-mRNA splicing; associates with thespliceosome and interacts with splicing factorsPrp22p and Prp46p; orthologous to humantranscriptional coactivator SKIP and canactivate transcription of a reporter gene 0.003320.05602&radic&radic&radic S000005068NAF1YNL124WverifiedProtein required for the assembly of box H/ACA snoRNPs and forpre-rRNA processing, forms a complex with Shq1pand interacts with H/ACA snoRNP componentsNhp2p and Cbf5p; has similarity to Gar1p andother RNA-binding proteins 0.00330.05584&radic S000007235SEM1YDR363W-AverifiedComponent of the lid subcomplex of the regulatory subunit ofthe 26S proteasome; ortholog of human DSS1 0.003290.05549&radic S000001026SBP1YHL034CverifiedNucleolar single-strand nucleic acid binding protein;associates with small nuclear RNAs 0.003270.05519&radic&radic&radic&radic&radic S000002292PPH21YDL134CverifiedCatalytic subunit of protein phosphatase 2A, functionallyredundant with Pph22p; methylated at Cterminus; forms alternate complexes withseveral regulatory subunits; involved in signaltransduction and regulation of mitosis 0.003260.05515&radic S000004490TSA1YML028WverifiedUbiquitous housekeeping thioredoxin peroxidase, reducesreactive oxygen, nitrogen and sulfur speciesusing thioredoxin as hydrogen donor; mediatesredox regulation of the nuclear localization ofYap1p; deletion results in mutator phenotype 0.003250.0551 S000004989YIP3YNL044WverifiedProtein localized to COPII vesicles, proposed to be involved inER to Golgi transport; interacts with membersof the Rab GTPase family and Yip1p; alsointeracts with Rtn1p 0.003250.05506 S000002209LHP1YDL051WverifiedRNA binding protein required for maturation of tRNA and snRNAprecursors; acts as a molecular chaperone forRNAs transcribed by polymerase III; homologousto human La (SS-B) autoantigen 0.003240.05498&radic&radic&radic S000002979SCL1YGL011CverifiedAlpha subunit of the 20S core complex of the 26S proteasomeinvolved in the degradation of ubiquitinatedsubstrates; essential for growth; detected inthe mitochondria 0.003230.05484&radic&radic S000000834FIR1YER032WverifiedProtein involved in 3' mRNA processing, interacts with Ref2p;potential Cdc28p substrate &radic0.003210.05462&radic&radic S000002505GRX3YDR098CverifiedHydroperoxide and superoxide-radical responsiveglutathione-dependent oxidoreductase; monothiolglutaredoxin subfamily member along with Grx4pand Grx5p; protects cells from oxidative damage 0.003210.05395&radic&radic&radic S000004119DIP2YLR129WverifiedNucleolar protein, specifically associated with the U3 snoRNA,part of the large ribonucleoprotein complexknown as the small subunit (SSU) processome,required for 18S rRNA biogenesis, part of theactive pre-rRNA processing complex 0.00320.05395&radic&radic&radic S000001744CAF4YKR036CverifiedWD40 repeat-containing protein associated with the CCR4-NOTcomplex, interacts in a Ccr4p-dependent mannerwith Ssn2p; also interacts with Fis1p, Mdv1pand Dnm1p and plays a role in mitochondrialfission 0.003190.05395 S000006105MRN1YPL184CuncharacterizedPutative RNA binding protein 0.00320.05395&radic&radic&radic S000002790RPP2BYDR382WverifiedRibosomal protein P2 beta, a component of the ribosomal stalk,which is involved in the interaction betweentranslational elongation factors and theribosome; regulates the accumulation of P1(Rpp1Ap and Rpp1Bp) in the cytoplasm 0.003170.05395&radic&radic&radic S000002719TFB1YDR311WverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, required for nucleotide excisionrepair, target for transcriptional activators 0.003210.05395&radic&radic&radic S000000747URA3YEL021WverifiedOrotidine-5'-phosphate (OMP) decarboxylase, catalyzes the sixthenzymatic step in the de novo biosynthesis ofpyrimidines, converting OMP into uridinemonophosphate (UMP); converts 5-FOA into5-fluorouracil, a toxic compound 0.003190.05395 S000004762NUP53YMR153WverifiedSubunit of the nuclear pore complex (NPC), interacts withkaryopherin Kap121p or with Nup170p viaoverlapping regions of Nup53p, involved inactivation of the spindle checkpoint mediatedby the Mad1p-Mad2p complex 0.003120.05306&radic&radic S000006278TKL1YPR074CverifiedTransketolase, similar to Tkl2p; catalyzes conversion ofxylulose-5-phosphate and ribose-5-phosphate tosedoheptulose-7-phosphate andglyceraldehyde-3-phosphate in the pentosephosphate pathway; needed for synthesis ofaromatic amino acids 0.003140.05306&radic S000003367PRE9YGR135Wverified20S proteasome beta-type subunit; the only nonessential 20Ssubunit 0.003140.05306&radic&radic&radic S000000044YAL046CUncharacterizedHypothetical protein 0.003130.05306&radic S000003060NUP145YGL092WverifiedEssential nucleoporin, catalyzes its own cleavage in vivo togenerate a C-terminal fragment that assemblesinto the Nup84p subcomplex of the nuclear porecomplex, and an N-terminal fragment of unknownfunction that is homologous to Nup100p 0.003120.05303&radic S000001404CCT2YIL142WverifiedSubunit beta of the cytosolic chaperonin Cct ring complex,related to Tcp1p, required for the assembly ofactin and tubulins in vivo 0.00310.05269 S000001447PRI1YIR008CverifiedSubunit of DNA primase, which is required for DNA synthesis anddouble-strand break repair 0.003090.05265 S000000993RPL14BYHL001WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl14Ap and has similarity to ratL14 ribosomal protein 0.003070.05211 S000004738RRB1YMR131CverifiedEssential nuclear protein involved in early steps of ribosomebiogenesis; physically interacts with theribosomal protein Rpl3p 0.003070.05211&radic S000002856ADA2YDR448WverifiedTranscription coactivator, component of the ADA and SAGAtranscriptional adaptor/HAT (histoneacetyltransferase) complexes 0.003070.05211&radic&radic S000000893MET6YER091CverifiedCobalamin-independent methionine synthase, involved in aminoacid biosynthesis; requires a minimum of twoglutamates on the methyltetrahydrofolatesubstrate, similar to bacterial metE homologs 0.003080.05211 S000005711GSP2YOR185CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; interacts withKap121p, Kap123p and Pdr6p (karyophilin betas);Gsp1p homolog that is not required forviability 0.003070.05211&radic&radic S000003648MDV1YJL112WverifiedPeripheral protein of the cytosolic face of the mitochondrialouter membrane, required for mitochondrialfission; interacts with Fis1p and with thedynamin-related GTPase Dnm1p; contains WDrepeats 0.003050.05203 S000002358YDL199CUncharacterizedPutative protein of unknown function with sequence similarity to members of the sugar permease family 0.003050.05203 S000000741EDC3YEL015WverifiedNon-essential conserved protein of unknown function, plays arole in mRNA decapping by specificallyaffecting the function of the decapping enzymeDcp1p; localizes to cytoplasmic mRNA processingbodies 0.003060.05203&radic&radic S000003236PEX31YGR004WverifiedPeroxisomal integral membrane protein, involved in negativeregulation of peroxisome size; partiallyfunctionally redundant with Pex30p and Pex32p;probably acts at a step downstream of stepsmediated by Pex28p and Pex29p 0.003050.052&radic&radic S000000929LCP5YER127WverifiedEssential protein involved in maturation of 18S rRNA; depletionleads to inhibited pre-rRNA processing andreduced polysome levels; localizes primarily tothe nucleolus 0.003050.05187&radic S000006090MEX67YPL169CverifiedPoly(A)RNA binding protein involved in nuclear mRNA export,component of the nuclear pore; ortholog ofhuman TAP 0.003050.05187&radic S000000409KTR3YBR205WverifiedPutative alpha-1,2-mannosyltransferase involved in O- andN-linked protein glycosylation; member of theKRE2/MNT1 mannosyltransferase family 0.003030.05175&radic&radic S000000322TEF2YBR118WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF1; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.003030.05162 S000006316MRD1YPR112CverifiedEssential conserved protein that associates with 35S precursorrRNA and is required for its initial processingat the A(0)-A(2) cleavage sites, shows partialnucleolar localization, contains five consensusRNA-binding domains 0.003020.05143&radic&radic&radic S000000370TYR1YBR166CverifiedPrephenate dehydrogenase involved in tyrosine biosynthesis,expression is dependent on phenylalanine levels 0.003020.05143 S000002913PSP1YDR505CverifiedAsn and gln rich protein of unknown function; high-copysuppressor of POL1 (DNA polymerase alpha) andpartial suppressor of CDC2 (polymerase delta)and CDC6 (pre-RC loading factor) mutations;overexpression results in growth inhibition 0.003010.05122&radic S000001057MIP6YHR015WverifiedPutative RNA-binding protein, interacts with Mex67p, which is acomponent of the nuclear pore involved innuclear mRNA export 0.0030.051&radic&radic&radic&radic S000004636ARP9YMR033WverifiedActin-related protein involved in transcriptional regulation;subunit of the chromatin remodeling Snf/Swicomplex 0.002980.0508 S000004949HRB1YNL004WverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toGbp2p and Npl3p 0.002980.0508&radic&radic&radic S000004520IMD4YML056CverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.002980.0508 S000002412MAF1YDR005CverifiedNegative regulator of RNA polymerase III; component of severalsignaling pathways that repress polymerase IIItranscription in response to changes incellular environment; targets the initiationfactor TFIIIB 0.002960.05065&radic&radic S000001876LPD1YFL018CverifiedDihydrolipoamide dehydrogenase, the lipoamide dehydrogenasecomponent (E3) of the pyruvate dehydrogenaseand 2-oxoglutarate dehydrogenase multi-enzymecomplexes 0.002970.05065&radic&radic S000001928YFR032CUncharacterizedPutative protein of unknown function; transcribed during sporulation; YFR032C is not an essential gene 0.002940.05034&radic&radic S000005101IGO1YNL157WuncharacterizedHypothetical protein 0.002950.05034&radic&radic S000004708YMR102CUncharacterizedProtein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene 0.002940.05002 S000003825CCT5YJR064WverifiedSubunit of the cytosolic chaperonin Cct ring complex, relatedto Tcp1p, required for the assembly of actinand tubulins in vivo 0.002930.05002 S000003608PSF2YJL072CverifiedSubunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), whichis localized to DNA replication origins andimplicated in assembly of the DNA replicationmachinery 0.002940.05002 S000002326NRP1YDL167CverifiedProtein of unknown function, rich in asparagine residues 0.00290.04957&radic&radic S000003084CDC20YGL116WverifiedCell-cycle regulated activator of anaphase-promotingcomplex/cyclosome (APC/C), which is requiredfor metaphase/anaphase transition; directsubiquitination of mitotic cyclins, Pds1p, andother anaphase inhibitors; potential Cdc28psubstrate 0.00290.04957&radic S000001195SPO12YHR152WverifiedNucleolar protein of unknown function, positive regulator ofexit from mitosis; involved in regulating therelease of Cdc14p from the nucleolus in earlyanaphase; proposed to play similar role inmeiosis 0.002890.04952&radic&radic&radic S000005152YNL208WUncharacterizedHypothetical protein 0.002890.04952 S000004881PRP24YMR268CverifiedSplicing factor that reanneals U4 and U6 snRNPs duringspliceosome recycling 0.002890.04952&radic&radic&radic&radic&radic S000002946PAD1YDR538WverifiedPhenylacrylic acid decarboxylase, confers resistance tocinnamic acid, decarboxylates aromaticcarboxylic acids to the corresponding vinylderivatives 0.002880.04945 S000002172NOP1YDL014WverifiedNucleolar protein, component of the small subunit processomecomplex, which is required for processing ofpre-18S rRNA; has similarity to mammalianfibrillarin 0.002870.04922&radic S000002773ESF1YDR365CverifiedNucleolar protein involved in pre-rRNA processing; depletioncauses severely decreased 18S rRNA levels 0.002880.04922&radic&radic&radic&radic&radic S000001734GCN3YKR026CverifiedAlpha subunit of the translation initiation factor eIF2B, theguanine-nucleotide exchange factor for eIF2;activity subsequently regulated byphosphorylated eIF2; first identified as apositive regulator of GCN4 expression 0.002830.04864&radic&radic S000005504NOP8YOL144WverifiedNucleolar protein required for 60S ribosomal subunit biogenesis 0.002860.04864&radic&radic S000005795PAC1YOR269WverifiedProtein involved in nuclear migration, part of thedynein/dynactin pathway; targets dynein tomicrotubule tips, which is necessary forsliding of microtubules along bud cortex;synthetic lethal with bni1; homolog of humanLIS1 0.002850.04864&radic S000003628HPR5YJL092WverifiedDNA helicase and DNA-dependent ATPase involved in DNA repair,required for proper timing of commitment tomeiotic recombination and the transition fromMeiosis I to Meiosis II; potential Cdc28psubstrate 0.002860.04864&radic S000000451ENP1YBR247CverifiedProtein associated with U3 and U14 snoRNAs, required forpre-rRNA processing and 40S ribosomal subunitsynthesis; localized in the nucleus andconcentrated in the nucleolus 0.002840.04864&radic&radic&radic&radic&radic S000004917YME2YMR302CverifiedIntegral inner mitochondrial membrane protein with similarityto exonucleases; mutants exhibit an increasedrate of mitochondrial DNA escape 0.002840.04864&radic&radic S000002675CIA1YDR267CverifiedEssential protein involved in assembly of cytosolic and nucleariron-sulfur proteins 0.002840.04864 S000006246PUF2YPR042CverifiedMember of the PUF protein family, which is defined by thepresence of Pumilio homology domains thatconfer RNA binding activity; preferentiallybinds mRNAs encoding membrane-associatedproteins 0.002840.04864&radic&radic S000000197ECM21YBL101CverifiedNon-essential protein of unknown function; promoter containsseveral Gcn4p binding elements 0.002860.04864 S000006348NOC4YPR144CverifiedNucleolar protein, forms a complex with Nop14p that mediatesmaturation and nuclear export of 40S ribosomalsubunits 0.002830.04857&radic&radic&radic&radic S000002240RPL13AYDL082WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl13Bp; not essential forviability; has similarity to rat L13 ribosomalprotein 0.002820.04843 S000003105SEC27YGL137WverifiedEssential beta'-coat protein of the COPI coatomer, involved inER-to-Golgi and Golgi-to-ER transport; containsWD40 domains that mediate cargo selectiveinteractions; 45% sequence identity tomammalian beta'-COP 0.002810.04821 S000001185RPC10YHR143W-AverifiedRNA polymerase subunit, found in RNA polymerase complexes I,II, and III 0.002770.04779&radic S000003482YGR250CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.002770.04779&radic&radic S000004416SPP382YLR424WverifiedEssential protein that forms a dimer with Ntr2p; also forms atrimer, with Ntr2p and Prp43p, that is involvedin spliceosome disassembly; found also in amultisubunit complex with the splicing factorClf1p; suppressor of prp38-1 mutation 0.002790.04779&radic S000000147PIN4YBL051CverifiedProtein involved in G2/M phase progression and response to DNAdamage, interacts with Rad53p; contains an RNArecognition motif, a nuclear localizationsignal, and several SQ/TQ cluster domains;hyperphosphorylated in response to DNA damage 0.002780.04779&radic&radic&radic S000005036YNL092WUncharacterizedPutative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene 0.002760.04763&radic S000001885CDC4YFL009WverifiedF-box protein required for G1/S and G2/M transition, associateswith Skp1p and Cdc53p to form a complex,SCFCdc4, which acts as ubiquitin-protein ligasedirecting ubiquitination of the phosphorylatedCDK inhibitor Sic1p 0.002740.04734&radic S000002466UBC5YDR059CverifiedUbiquitin-conjugating enzyme that mediates selectivedegradation of short-lived and abnormalproteins, central component of the cellularstress response; expression is heat inducible 0.002720.04697&radic&radic S000001489RPL14AYKL006WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl14Bpand has similarity to rat L14 ribosomalprotein; rpl14a csh5 double null mutantexhibits synthetic slow growth 0.002720.04697 S000002837TIF35YDR429CverifiedSubunit of the core complex of translation initiation factor3(eIF3), which is essential for translation 0.002710.04697&radic&radic&radic S000003572TAD2YJL035CverifiedSubunit of tRNA-specific adenosine-34 deaminase, forms aheterodimer with Tad3p that converts adenosineto inosine at the wobble position of severaltRNAs 0.00270.04685&radic S000004585NAB6YML117WuncharacterizedPutative RNA-binding protein, based on computational analysisof large-scale protein-protein interaction data 0.002690.04657&radic&radic S000006104YPL183CUncharacterizedHypothetical protein 0.002650.04617 S000002304COP1YDL145CverifiedAlpha subunit of COPI vesicle coatomer complex, which surroundstransport vesicles in the early secretorypathway 0.002650.04617 S000005013RPL16BYNL069CverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, binds to 5.8 S rRNA; hassimilarity to Rpl16Ap, E. coli L13 and rat L13aribosomal proteins; transcriptionally regulatedby Rap1p 0.002650.04614 S000006367TIF3YPR163CverifiedTranslation initiation factor eIF-4B, has RNA annealingactivity; contains an RNA recognition motif andbinds to single-stranded RNA 0.002650.04614&radic&radic&radic S000002211PBP4YDL053CverifiedPbp1p binding protein, interacts strongly with Pab1p-bindingprotein 1 (Pbp1p) in the yeast two-hybridsystem; also interacts with Lsm12p in acopurification assay 0.002630.04595&radic S000001229KOG1YHR186CverifiedSubunit of TORC1, a rapamycin-sensitive complex involved ingrowth control that contains Tor1p or Tor2p,Lst8p and Tco89p; contains four HEAT repeatsand seven WD-40 repeats; may act as a scaffoldprotein to couple TOR and its effectors 0.002630.04584&radic&radic S000006060UME1YPL139CverifiedNegative regulator of meiosis, required for repression of asubset of meiotic genes during vegetativegrowth, binding of histone deacetylase Rpd3prequired for activity, contains a NEE box and aWD repeat motif; homologous with Wtm1p, Wtm2p 0.002620.04582&radic&radic&radic S000001016RIM4YHL024WverifiedPutative RNA-binding protein required for the expression ofearly and middle sporulation genes 0.00260.04544&radic&radic S000004722ASC1YMR116CverifiedWD repeat protein (G-beta like protein) involved in translationregulation; required for repression of Gcn4pactivity in the absence of amino-acidstarvation; core component of the ribosome;ortholog of mammalian RACK1 0.00260.04544 S000002491TVP23YDR084CuncharacterizedIntegral membrane protein localized to late Golgi vesiclesalong with the v-SNARE Tlg2p; green fluorescentprotein (GFP)-fusion protein localizes to thecytoplasm in a punctate pattern 0.002610.04544&radic S000000544GID7YCL039WverifiedProtein of unknown function, involved in proteasome-dependentcatabolite inactivation offructose-1,6-bisphosphatase; contains six WD40repeats; computational analysis suggests thatGid7p and Moh1p have similar functions 0.00260.04544&radic&radic&radic S000004903HAS1YMR290CverifiedATP-dependent RNA helicase; localizes to both the nuclearperiphery and nucleolus; highly enriched innuclear pore complex fractions; constituent of66S pre-ribosomal particles 0.002590.04535&radic&radic&radic&radic S000002732UTP4YDR324CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.002590.04535&radic&radic&radic&radic S000001584HSL1YKL101WverifiedNim1p-related protein kinase that regulates the morphogenesisand septin checkpoints; associates with theassembled septin filament; required along withHsl7p for bud neck recruitment,phosphorylation, and degradation of Swe1p 0.002570.04509&radic&radic&radic S000002319DHH1YDL160CverifiedCytoplasmic DExD/H-box helicase, stimulates mRNA decapping,coordinates distinct steps in mRNA function anddecay, interacts with both the decapping anddeadenylase complexes, may have a role in mRNAexport and translation 0.002580.04509&radic S000002452RPC11YDR045CverifiedRNA polymerase III subunit C11; mediates pol III RNA cleavageactivity and is important for termination oftranscription 0.002560.04497 S000000868YER066WUncharacterizedHypothetical protein 0.002540.04463 S000006021ATG21YPL100WverifiedPhosphatidylinositol 3,5-bisphosphate-binding protein requiredfor maturation of pro-aminopeptidase I,predicted to fold as a seven-bladedbeta-propeller; displays punctate cytoplasmiclocalization 0.002530.04439&radic S000005334BRE5YNR051CverifiedUbiquitin protease cofactor, forms deubiquitination complexwith Ubp3p that coregulates anterograde andretrograde transport between the endoplasmicreticulum and Golgi compartments; null issensitive to brefeldin A 0.002530.04439&radic&radic S000006253ATG11YPR049CverifiedPeripheral membrane protein required for delivery ofaminopeptidase I (Lap4p) to the vacuole in thecytoplasm-to-vacuole targeting pathway; alsorequired for peroxisomal degradation(pexophagy) 0.002510.04422 S000004045SPT8YLR055CverifiedSubunit of the SAGA transcriptional regulatory complex but notpresent in SAGA-like complex SLIK/SALSA,required for SAGA-mediated inhibition at somepromoters 0.002490.04391&radic&radic&radic S000003360UTP8YGR128CverifiedNucleolar protein required for export of tRNAs from thenucleus; also copurifies with the small subunit(SSU) processome containing the U3 snoRNA thatis involved in processing of pre-18S rRNA 0.002470.04373&radic&radic&radic S000006382PRP4YPR178WverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.002460.04354&radic&radic S000006341RRP9YPR137WverifiedProtein involved in pre-rRNA processing, associated with U3snRNP; component of small ribosomal subunit(SSU) processosome; ortholog of the humanU3-55k protein 0.002460.04346&radic&radic&radic&radic S000005141WHI3YNL197CverifiedRNA binding protein that sequesters CLN3 mRNA in cytoplasmicfoci; cytoplasmic retention factor for Cdc28pand associated cyclins; regulates cell fate anddose-dependently regulates the critical cellsize required for passage through Start 0.002450.04343&radic&radic&radic S000000416NGR1YBR212WverifiedRNA binding protein that negatively regulates growth rate;interacts with the 3' UTR of the mitochondrialporin (POR1) mRNA and enhances its degradation;overexpression impairs mitochondrial function;expressed in stationary phase 0.002450.04339&radic&radic&radic S000000870MOT2YER068WverifiedComponent of the CCR4-NOT complex, which has multiple roles intranscription regulation, mRNA degradation, andpost-transcriptional modifications; with Ubc4p,ubiquitylates subunits of the nascentpolypeptide-associated complex (NAC) 0.002440.04313&radic&radic&radic S000000782HAT2YEL056WverifiedSubunit of the Hat1p-Hat2p histone acetyltransferase complex;required for high affinity binding of thecomplex to free histone H4, thereby enhancingHat1p activity; similar to human RbAp46 and 48;has a role in telomeric silencing 0.002440.04313&radic S000004139YLR149CUncharacterizedPutative protein of unknown function; YLR149C is not an essential gene 0.002420.04281&radic S000003851JSN1YJR091CverifiedMember of the Puf family of RNA-binding proteins, interactswith mRNAs encoding membrane-associatedproteins; overexpression suppresses a tub2-150mutation and causes increased sensitivity tobenomyl in wild-type cells 0.00240.04252&radic&radic S000003092MON1YGL124CverifiedProtein required for fusion of cvt-vesicles and autophagosomeswith the vacuole; associates, as a complex withCcz1p, with a perivacuolar compartment;potential Cdc28p substrate 0.002410.04252 S000004439VMA6YLR447CverifiedSubunit D of the five-subunit V0 integral membrane domain ofvacuolar H+-ATPase (V-ATPase), an electrogenicproton pump found in the endomembrane system;stabilizes VO subunits; required for V1 domainassembly on the vacuolar membrane 0.00240.04252 S000000238HMT1YBR034CverifiedNuclear SAM-dependent mono- and asymmetric argininedimethylating methyltransferase that modifieshnRNPs, including Npl3p and Hrp1p, thusfacilitating nuclear export of these proteins;required for viability of npl3 mutants 0.00240.04252&radic&radic S000000945DDI1YER143WverifiedDNA damage-inducible v-SNARE binding protein, contains aubiquitin-associated (UBA) domain, may act as anegative regulator of constitutive exocytosis,may play a role in S-phase checkpoint control 0.002370.04203 S000004429YLR437CUncharacterizedPutative protein of unknown function; epitope tagged protein localizes to the cytoplasm 0.002350.04167 S000005029MKT1YNL085WverifiedProtein that forms a complex with Pbp1p that may mediateposttranscriptional regulation of HOendonuclease; involved in propagation of M2dsRNA satellite of L-A virus 0.002330.04137 S000004652ERB1YMR049CverifiedProtein required for maturation of the 25S and 5.8S ribosomalRNAs; constituent of 66S pre-ribosomalparticles; homologous to mammalian Bop1 0.002330.04137&radic S000002377YDL218WUncharacterizedHypothetical protein 0.002330.04137 S000004122YLR132CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to mitochondria and nucleus; YLR132C is an essential gene 0.002320.04126&radic S000005398PRE6YOL038Wverified20S proteasome alpha-type subunit; green fluorescent protein(GFP)-fusion protein relocates from the cytosolto the mitochondrial outer surface uponoxidative stress 0.00230.04077&radic&radic&radic S000003874RSM7YJR113CverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S7 ribosomal protein 0.00230.04077 S000003871YMR1YJR110WverifiedPhosphatidylinositol 3-phosphate [PI(3)P] phosphatase,regulates the localization and levels ofPI(3)P; involved in cytoplasm to vacuole (CVT)transport; has similarity to the conservedmyotubularin dual specificity phosphatasefamily 0.002260.04025 S000002487VPS41YDR080WverifiedVacuolar membrane protein that is a subunit of the homotypicvacuole fusion and vacuole protein sorting(HOPS) complex; essential for membrane dockingand fusion at the Golgi-to-endosome andendosome-to-vacuole stages of protein transport 0.002260.04011&radic&radic S000005131YNL187WUncharacterizedPutative protein of unknown function; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; may interact with or be fuctionally redundant with Prp40p 0.002260.04011&radic&radic S000001354YIL092WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus 0.002250.0399&radic S000000399MSI1YBR195CverifiedSubunit of chromatin assembly factor I (CAF-I), regulates theRAS/cAMP pathway via sequestration of Npr1pkinase; localizes to the nucleus and cytoplasm;homologous to human retinoblastoma bindingproteins RbAp48 and RbAp46 0.002230.03944&radic&radic S000000452HIS7YBR248CverifiedImidazole glycerol phosphate synthase (glutamineamidotransferase:cyclase), catalyzes the fifthand sixth steps of histidine biosynthesis andalso produces 5-aminoimidazole-4-carboxamideribotide (AICAR), a purine precursor 0.00220.03911&radic&radic&radic S000000680TUP1YCR084CverifiedGeneral repressor of transcription, forms complex with Cyc8p,involved in the establishment of repressivechromatin structure through interactions withhistones H3 and H4, appears to enhanceexpression of some genes 0.002190.03911&radic&radic S000003457AMA1YGR225WverifiedActivator of meiotic anaphase promoting complex (APC/C); Cdc20pfamily member; required for initiation of sporewall assembly; required for Clb1p degradationduring meiosis 0.002180.03899 S000005888PRT1YOR361CverifiedSubunit of the core complex of translation initiation factor3(eIF3), essential for translation; part of asubcomplex (Prt1p-Rpg1p-Nip1p) that stimulatesbinding of mRNA and tRNA(i)Met to ribosomes 0.002180.03893&radic&radic&radic&radic S000004186PWP1YLR196WverifiedProtein with WD-40 repeats involved in rRNA processing;associates with trans-acting ribosomebiogenesis factors; similar to beta-transducinsuperfamily 0.002180.03893&radic&radic&radic S000004415ATG17YLR423CverifiedProtein that interacts with and is required for activation ofApg1p protein kinase; involved in autophagy butnot in the Cvt (cytoplasm to vacuole targeting)pathway 0.002170.03887 S000006339CTF4YPR135WverifiedChromatin-associated protein, required for sister chromatidcohesion; interacts with DNA polymerase alpha(Pol1p) and may link DNA synthesis to sisterchromatid cohesion 0.002160.03861&radic&radic S000002824SYF1YDR416WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression; similar to Drosophila crooked neckprotein 0.002150.03854&radic S000001177YCK1YHR135CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck2p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck2p 0.002140.03849&radic&radic S000000100UTP20YBL004WverifiedComponent of the small-subunit (SSU) processome, which isinvolved in the biogenesis of the 18S rRNA 0.002150.03849&radic&radic S000000920SHO1YER118CverifiedTransmembrane osmosensor, participates in activation of boththe Cdc42p- and MAP kinase-dependentfilamentous growth pathway and thehigh-osmolarity glycerol response pathway 0.002130.0382 S000003181SKI8YGL213CverifiedProtein involved in exosome mediated 3' to 5' mRNA degradationand translation inhibition of non-poly(A) mRNAsas well as double-strand break formation duringmeiotic recombination; required for repressingpropagation of dsRNA viruses 0.002120.03804&radic&radic S000000542SRO9YCL037CverifiedCytoplasmic RNA-binding protein that associates withtranslating ribosomes; involved in hemeregulation of Hap1p as a component of the HMCcomplex, also involved in the organization ofactin filaments; contains a La motif 0.002110.0378&radic&radic S000004570CAC2YML102WverifiedComponent of the chromatin assembly complex (with Rlf2p andMsi1p) that assembles newly synthesizedhistones onto recently replicated DNA, requiredfor building functional kinetochores, conservedfrom yeast to humans 0.00210.0378&radic S000005928TFC8YPL007CverifiedOne of six subunits of RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part ofTFIIIC TauB domain that binds BoxB promotersites of tRNA and other genes; linker betweenTauB and TauA domains; human homolog isTFIIIC-90 0.002090.03754&radic&radic&radic S000004723SPC24YMR117CverifiedComponent of the evolutionarily conservedkinetochore-associated Ndc80 complex(Ndc80p-Nuf2p-Spc24p-Spc25p); involved inchromosome segregation, spindle checkpointactivity and kinetochore clustering 0.002080.03754 S000005205ORC5YNL261WverifiedSubunit of the origin recognition complex, which directs DNAreplication by binding to replication originsand is also involved in transcriptionalsilencing 0.002050.03696&radic&radic S000004176EMG1YLR186WverifiedProtein required for the maturation of the 18S rRNA and for 40Sribosome production; associated withspindle/microtubules; nuclear localizationdepends on physical interaction with Nop14p;may bind snoRNAs 0.002040.03666&radic&radic S000004698AIP1YMR092CverifiedActin cortical patch component, interacts with the actindepolymerizing factor cofilin; required torestrict cofilin localization to corticalpatches; contains WD repeats 0.002030.03666&radic S000002437RAD28YDR030CverifiedProtein involved in transcription-coupled repair nucleotideexcision repair of UV-induced DNA lesions;homolog of human CSA protein 0.002030.03666 S000005197TEX1YNL253WverifiedProtein involved in mRNA export, component of the transcriptionexport (TREX) complex 0.002020.03643 S000003414YGR182CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W 0.002010.03643&radic&radic S000003068SEH1YGL100WverifiedNuclear pore protein that is part of the evolutionarilyconserved Nup84p complex (Nup84p, Nup85p,Nup120p, Nup145p, and Seh1p); homologous toSec13p 0.002020.03643 S000003284FMP48YGR052WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.002020.03643&radic&radic S000001768UTP30YKR060WuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.002010.03643&radic&radic S000002740IRC3YDR332WuncharacterizedHypothetical protein; null mutant displays increased levels ofspontaneous Rad52 foci 0.0020.03607&radic&radic S000005126IPI3YNL182CuncharacterizedProtein required for cell viability; computational analysis oflarge-scale protein-protein interaction datasuggests a possible role in assembly of theribosomal large subunit 0.002010.03607&radic S000005309SEC12YNR026CverifiedGuanine nucleotide exchange factor (GEF); glycosylated integralmembrane protein of the endoplasmic reticulum,important for the initiation of transportvesicle budding from the endoplasmic reticulumthrough activation of the GTPase Sar1p 0.001990.03607 S000004951LST8YNL006WverifiedProtein required for the transport of amino acid permease Gap1pfrom the Golgi to the cell surface; componentof the TOR signaling pathway; associates withboth Tor1p and Tor2p; contains a WD-repeat 0.0020.03607 S000000202SRO77YBL106CverifiedProtein with roles in exocytosis and cation homeostasis;functions in docking and fusion of post-Golgivesicles with plasma membrane; homolog of Sro7pand Drosophila lethal giant larvae tumorsuppressor; interacts with SNARE protein Sec9p 0.001990.03607 S000005552BUB3YOR026WverifiedKinetochore checkpoint WD40 repeat protein that localizes tokinetochores during prophase and metaphase,delays anaphase in the presence of unattachedkinetochores; forms complexes with Mad1p-Bub1pand with Cdc20p, binds Mad2p and Mad3p 0.001980.03584&radic&radic S000002666HSP78YDR258CverifiedOligomeric mitochondrial matrix chaperone that cooperates withSsc1p in mitochondrial thermotolerance afterheat shock; prevents the aggregation ofmisfolded matrix proteins; component of themitochondrial proteolysis system 0.001980.03584&radic&radic S000006385SEC23YPR181CverifiedGTPase-activating protein; component of the Sec23p-Sec24pheterodimeric complex of the COPII vesiclecoat, involved in ER to Golgi transport andautophagy; stimulates the GDP-bound form ofSar1p 0.001990.03584&radic S000001451SQT1YIR012WverifiedEssential protein involved in a late step of 60S ribosomalsubunit assembly or modification; containsmultiple WD repeats; interacts with Qsr1p in atwo-hybrid assay 0.001980.03584&radic S000004915ADE4YMR300CverifiedPhosphoribosylpyrophosphate amidotransferase (PRPPAT;amidophosphoribosyltransferase), catalyzesfirst step of the 'de novo' purine nucleotidebiosynthetic pathway 0.001970.03581&radic&radic S000005606DIA2YOR080WverifiedOrigin-binding F-box protein that forms an SCF ubiquitin ligasecomplex with Skp1p and Cdc53p; plays a role inDNA replication, involved in invasive andpseudohyphal growth 0.001970.03574&radic S000006010RLM1YPL089CverifiedMADS-box transcription factor, component of the protein kinaseC-mediated MAP kinase pathway involved in themaintenance of cell integrity; phosphorylatedand activated by the MAP-kinase Slt2p 0.001950.03537&radic&radic S000004301CDC25YLR310CverifiedMembrane bound guanine nucleotide exchange factor (GEF orGDP-release factor); indirectly regulatesadenylate cyclase through activation of Ras1pand Ras2p by stimulating the exchange of GDPfor GTP; required for progression through G1 0.001940.03524&radic&radic S000000200YBL104CUncharacterizedHypothetical protein 0.001940.03524 S000003090NAB2YGL122CverifiedNuclear polyadenylated RNA-binding protein; autoregulates mRNAlevels; related to human hnRNPs; has nuclearlocalization signal sequence that binds toKap104p; required for poly(A) tail lengthcontrol and nuclear mRNA export &radic0.001930.03506&radic&radic S000000628BPH1YCR032WverifiedPProtein homologous to human Chediak-Higashi syndrome andmurine Beige proteins, which are implicated indisease syndromes due to defective lysosomaltrafficking; mutant phenotype and geneticinteractions suggest a role in protein sorting 0.001920.03501 S000000099HTA2YBL003CverifiedOne of two nearly identical (see also HTA1) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.001910.03479&radic&radic&radic&radic&radic S000003494BUD32YGR262CverifiedProtein kinase proposed to be involved in bud-site selection,telomere uncapping and elongation, andtranscription; component of the EKC/KEOPSprotein complex with Kae1p, Cgi121p, Pcc1p, andGon7p 0.001910.03479&radic&radic&radic S000000909GLE2YER107CverifiedComponent of the nuclear pore complex required forpolyadenylated RNA export but not for proteinimport, homologous to S. pombe Rae1p 0.001910.0346 S000005693RPS28AYOR167CverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps28Bp and has similarity to ratS28 ribosomal protein 0.00190.0346&radic&radic S000002896PAC11YDR488CverifiedDynein intermediate chain, acts in the cytoplasmic dyneinpathway, forms cortical cytoplasmic microtubulecapture site with Num1p; null mutant isdefective in nuclear migration, essential inthe absence of CIN8 0.00190.0346 S000000464RGD1YBR260CverifiedGTPase-activating protein (RhoGAP) for Rho3p and Rho4p,possibly involved in control of actincytoskeleton organization 0.001890.03428&radic S000004553UFO1YML088WverifiedF-box receptor protein, subunit of the Skp1-Cdc53-F-boxreceptor (SCF) E3 ubiquitin ligase complex;binds to phosphorylated Ho endonuclease,allowing its ubiquitylation by SCF andsubsequent degradation 0.00190.03428 S000006124TPK2YPL203WverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; activates filamentous growth 0.001890.03428&radic&radic&radic S000002501YDR094WDubiousHypothetical protein 0.00190.03428&radic S000004857YMR244C-AUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR244C-A is not an essential gene 0.001890.03428&radic&radic S000000485DUG2YBR281CuncharacterizedProtein involved in alternative pathway of glutathionedegradation; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmand nucleus; YBR281C is not an essential gene 0.001880.03422&radic S000003794RAV1YJR033CverifiedSubunit of the RAVE complex (Rav1p, Rav2p, Skp1p), whichpromotes assembly of the V-ATPase holoenzyme;required for transport between the early andlate endosome/PVC and for localization of TGNmembrane proteins; potential Cdc28p substrate 0.001870.03403 S000005361PHO80YOL001WverifiedCyclin, negatively regulates phosphate metabolism;Pho80p-Pho85p (cyclin-CDK complex)phosphorylates Pho4p and Swi5p; deletion ofPHO80 leads to aminoglycoside supersensitivity;truncated form of PHO80 affects vacuoleinheritance 0.001870.03403&radic&radic S000002831CAD1YDR423CverifiedAP-1-like bZIP transcriptional activator involved in multiplestress responses, iron metabolism, andpleiotropic drug resistance; controls a set ofgenes involved in stabilizing proteins, bindsconsensus sequence TTACTAA; 5' UTR containsuORFs 0.001870.03403&radic S000005233PCL1YNL289WverifiedPho85 cyclin of the Pcl1,2-like subfamily, involved in entryinto the mitotic cell cycle and regulation ofmorphogenesis, localizes to sites of polarizedcell growth 0.001880.03403&radic&radic S000003605UTP18YJL069CuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.001870.03403&radic&radic S000006289YPR085CUncharacterizedProtein required for cell viability 0.001870.03403 S000000668RSA4YCR072CverifiedWD-repeat protein involved in ribosome biogenesis; may interactwith ribosomes; required for maturation andefficient intra-nuclear transport or pre-60Sribosomal subunits, localizes to the nucleolus 0.001870.03389&radic S000003428FYV8YGR196CverifiedProtein of unknown function, required for survival uponexposure to K1 killer toxin 0.001870.03389&radic&radic S000004067FMP25YLR077WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.001840.03324&radic&radic S000003411OKP1YGR179CverifiedOuter kinetochore protein, required for accurate mitoticchromosome segregation; component of thekinetochore sub-complex COMA (Ctf19p, Okp1p,Mcm21p, Ame1p) that functions as a platform forkinetochore assembly 0.001850.03324&radic&radic S000002632HTB1YDR224CverifiedOne of two nearly identical (see HTB2) histone H2B subtypesrequired for chromatin assembly and chromosomefunction; Rad6p-Bre1p-Lge1p mediatedubiquitination regulates transcriptionalactivation, meiotic DSB formation and H3methylation 0.001830.03324&radic&radic S000004980YNL035CUncharacterizedPutative protein of unknown function with similarity to proteins containing WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene 0.001830.03324&radic&radic S000003370TPO2YGR138CverifiedPolyamine transport protein specific for spermine; localizes tothe plasma membrane; transcription of TPO2 isregulated by Haa1p; member of the majorfacilitator superfamily 0.001850.03324&radic S000005148SPS18YNL204CverifiedProtein of unknown function, contains a putative zinc-bindingdomain; expressed during sporulation 0.001840.03324&radic&radic&radic S000002604YDR196CUncharacterizedpredicted to catalyze the final step in synthesis of Coenzyme A 0.001830.03324&radic&radic&radic S000003349YGR117CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.001820.03306 S000003340CLB1YGR108WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 0.001820.03302&radic&radic&radic S000005732NOC2YOR206WverifiedProtein that forms a nucleolar complex with Mak21p that bindsto 90S and 66S pre-ribosomes, as well as anuclear complex with Noc3p that binds to 66Spre-ribosomes; both complexes mediateintranuclear transport of ribosomal precursors 0.001810.03294&radic&radic&radic S000000926DSE1YER124CverifiedDaughter cell-specific protein, may participate in pathwaysregulating cell wall metabolism; deletionaffects cell separation after division andsensitivity to drugs targeted against the cellwall 0.001810.03281 S000000552YCL047CUncharacterizedPutative protein of unknown function 0.001810.03281&radic&radic S000004368PSY3YLR376CuncharacterizedProtein of unknown function; deletion results in a mutatorphenotype suggesting a role for this protein asa mutational suppressor; deletion increasessensitivity to anticancer drugs oxaliplatin andcisplatin but not mitomycin C 0.001810.03277&radic&radic S000004844PEP5YMR231WverifiedPeripheral vacuolar membrane protein required for proteintrafficking and vacuole biogenesis; formscomplex with Pep3p that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, also interacts with Pep7p 0.00180.03267 S000001153UBA4YHR111WverifiedProtein that activates Urm1p before its conjugation to proteins(urmylation); one target is the thioredoxinperoxidase Ahp1p, suggesting a role ofurmylation in the oxidative stress response 0.001780.03229&radic S000006236SRO7YPR032WverifiedPutative effector of Rab GTPase Sec4p, forms a complex withSec4p and t-SNARE Sec9p; involved in exocytosisand docking and fusion of post-Golgi vesicleswith plasma membrane; homolog of Sro77p andDrosophila lgl tumor suppressor 0.001790.03229 S000000129RIB1YBL033CverifiedGTP cyclohydrolase II; catalyzes the first step of theriboflavin biosynthesis pathway 0.001790.03229&radic&radic S000005768SSP2YOR242CverifiedSporulation specific protein that localizes to the spore wall;required for sporulation at a point aftermeiosis II and during spore wall formation;SSP2 expression is induced midway in meiosis 0.001780.03229&radic&radic&radic S000005147YNL203CDubiousHypothetical protein 0.001770.03204&radic S000000472MRPL37YBR268WverifiedMitochondrial ribosomal protein of the large subunit 0.001780.03204&radic S000005755WTM2YOR229WverifiedTranscriptional repressor involved in regulation of meiosis andsilencing; contains WD repeats 0.001770.03204&radic&radic&radic S000002669EXG2YDR261CverifiedExo-1,3-beta-glucanase, involved in cell wall beta-glucanassembly; may be anchored to the plasmamembrane via a glycosylphosphatidylinositol(GPI) anchor 0.001760.0319&radic&radic S000005694GLN4YOR168WverifiedGlutamine tRNA synthetase, monomeric class I tRNA synthetasethat catalyzes the specific glutaminylation oftRNA(Glu); N-terminal domain proposed to beinvolved in enzyme-tRNA interactions 0.001770.0319&radic&radic S000004255NEJ1YLR265CverifiedProtein involved in regulation of nonhomologous end joining;repressed by MAT heterozygosity; associateswith Lif1p and regulates its cellulardistribution 0.001770.0319&radic S000003079NSA1YGL111WverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis 0.001760.03186&radic S000005258DAL82YNL314WverifiedPositive regulator of allophanate inducible genes; binds adodecanucleotide sequence upstream of all genesthat are induced by allophanate; contains anUISALL DNA-binding, a transcriptionalactivation, and a coiled-coil domain 0.001750.03155&radic S000000301VPS15YBR097WverifiedMyristoylated serine/threonine protein kinase involved invacuolar protein sorting; functions as amembrane-associated complex with Vps34p; activeform recruits Vps34p to the Golgi membrane;interacts with the GDP-bound form of Gpa1p 0.001750.03155 S000005429HST1YOL068CverifiedNAD(+)-dependent histone deacetylase; essential subunit of theSum1p/Rfm1p/Hst1p complex required forORC-dependent silencing and mitotic repression;non-essential subunit of the Set3C deacetylasecomplex; involved in telomere maintenance 0.001740.03125&radic&radic&radic S000004066YLR076CDubiousHypothetical protein; open reading frame overlaps essential RPL10 gene encoding the ribosomal protein L10 which is a component of the large (60S) ribosomal subunit and responsible for joining the large and small subunits 0.001740.03125&radic&radic S000003287MUP1YGR055WverifiedHigh affinity methionine permease, integral membrane proteinwith 13 putative membrane-spanning regions;also involved in cysteine uptake 0.001740.03125&radic&radic S000000203YBL107CUncharacterizedHypothetical protein 0.001730.03125&radic S000002836YDR428CUncharacterizedPutative alpha/beta-serine hydrolase belonging to the subfamily of hormone-sensitive lipase-like hydrolases 0.001730.03098 S000006386SMX3YPR182WverifiedCore Sm protein Sm F; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm F 0.001730.03098&radic&radic&radic S000001870EPL1YFL024CverifiedComponent of NuA4, which is an essential histone H4/H2Aacetyltransferase complex; homologous toDrosophila Enhancer of Polycomb 0.001730.03098&radic&radic S000002534ARO1YDR127WverifiedPentafunctional arom protein, catalyzes steps 2 through 6 inthe biosynthesis of chorismate, which is aprecursor to aromatic amino acids 0.001710.0305&radic&radic S000003212TAD1YGL243WverifiedtRNA-specific adenosine deaminase, deaminates adenosine-37 toinosine in tRNA-Ala 0.001710.0305&radic&radic S000000047YAL049CUncharacterizedHypothetical protein 0.001710.0305&radic&radic&radic S000004870PET111YMR257CverifiedSpecific translational activator for the COX2 mRNA, located inthe mitochondrial inner membrane 0.00170.0305&radic S000005685SME1YOR159CverifiedCore Sm protein Sm E; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm E 0.00170.03035&radic&radic&radic S000005785RPT4YOR259CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for spindlepole body duplication; localized mainly to thenucleus throughout the cell cycle 0.001690.03021&radic&radic&radic&radic S000004156SEC10YLR166CverifiedEssential 100kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 0.001690.03021&radic&radic S000002838CYM1YDR430CverifiedLysine-specific metalloprotease of the mitochondrialintermembrane space, member of the pitrilysinfamily; degrades proteins and presequencepeptides cleaved from imported proteins;required for normal mitochondrial morphology 0.001690.03021&radic S000001142YHR100CUncharacterizedPutative protein of unknown function; exhibits growth defect on a non-fermentable (respiratory) carbon source; YHR100C is not an essential gene 0.001690.03002 S000000811NTF2YER009WverifiedNuclear envelope protein, interacts with GDP-bound Gsp1p andwith proteins of the nuclear pore to transportGsp1p into the nucleus where it is an essentialplayer in nucleocytoplasmic transport 0.001680.02976&radic S000000042GCV3YAL044CverifiedH subunit of the mitochondrial glycine decarboxylase complex,required for the catabolism of glycine to5,10-methylene-THF; expression is regulated bylevels of levels of 5,10-methylene-THF in thecytoplasm 0.001680.02976&radic&radic S000000052GPB2YAL056WverifiedProposed beta subunit of the heterotrimeric G protein;interacts with the receptor Grp1p, affectssignaling by altering the level ofphosphorylation of PKS substrates; regulatespseudohyphal growth through cAMP levels;homolog of Gpb1p 0.001670.02955 S000005447YOL087CUncharacterizedHypothetical protein 0.001660.02924 S000003205MTO1YGL236CverifiedMitochondrial protein, forms a heterodimer complex with Mss1pthat performs the 5-carboxymethylaminomethylmodification of the wobble uridine base inmitochondrial tRNAs; required for respirationin paromomycin-resistant 15S rRNA mutants 0.001660.02924&radic&radic S000001028MUP3YHL036WverifiedLow affinity methionine permease, similar to Mup1p 0.001650.029&radic S000000392NTC20YBR188CverifiedMember of a complex, including Prp19p, that binds to thespliceosome; required for pre-mRNA splicing 0.001650.029&radic&radic&radic&radic S000000450YBR246WUncharacterizedHypothetical protein 0.001650.02838 S000003401PUS6YGR169CverifiedPseudouridine synthase responsible for modification ofcytoplasmic and mitochondrial tRNAs at position31; mutation of Asp168 to Ala abolishes enzymeactivity; not essential for viability 0.001650.02838&radic&radic&radic S000002535YDR128WUncharacterizedHypothetical protein 0.001640.02838&radic S000005611OST3YOR085WverifiedGamma subunit of the oligosaccharyltransferase complex of theER lumen, which catalyzes asparagine-linkedglycosylation of newly synthesized proteins;Ost3p is important for N-glycosylation of asubset of proteins 0.001640.02838&radic&radic S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 0.001630.02838&radic&radic&radic&radic S000001608RRN3YKL125WverifiedProtein required for transcription of rDNA by RNA polymerase I;transcription factor independent of DNAtemplate; involved in recruitment of RNApolymerase I to rDNA 0.001650.02838&radic&radic&radic S000005427RIB2YOL066CverifiedDRAP deaminase, catalyzes the third step of the riboflavinbiosynthesis pathway; cytoplasmic tRNApseudouridine synthase involved inpseudouridylation of cytoplasmic tRNAs atposition 32 0.001630.02739&radic&radic S000003169MCM6YGL201CverifiedProtein involved in DNA replication; component of the Mcm2-7hexameric complex that binds chromatin as apart of the pre-replicative complex 0.001620.02739&radic&radic&radic S000004125SLX4YLR135WverifiedSubunit of a complex, with Slx1p, that hydrolyzes 5' branchesfrom duplex DNA in response to stalled orconverging replication forks; function overlapswith that of Sgs1p-Top3p 0.001620.02739&radic&radic S000002743MSN5YDR335WverifiedKaryopherin involved in nuclear import and export; shown to beresponsible for nuclear import of replicationprotein A and for export of several proteinsincluding Swi6p, Far1p, and Pho4p; cargodissociation involves binding to RanGTP 0.001620.02739&radic&radic&radic S000006323CLB2YPR119WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 0.001620.02739&radic&radic S000005982ALD6YPL061WverifiedCytosolic aldehyde dehydrogenase, activated by Mg2+ andutilizes NADP+ as the preferred coenzyme;required for conversion of acetaldehyde toacetate; constitutively expressed; locates tothe mitochondrial outer surface upon oxidativestress 0.001610.02739&radic&radic S000000830MIG3YER028CverifiedProbable transcriptional repressor involved in response totoxic agents such as hydroxyurea that inhibitribonucleotide reductase; phosphorylation bySnf1p or the Mec1p pathway inactivates Mig3p,allowing induction of damage response genes 0.001610.02739&radic&radic&radic S000003455HSV2YGR223CverifiedPhosphatidylinositol 3,5-bisphosphate-binding protein,predicted to fold as a seven-bladedbeta-propeller; displays punctate cytoplasmiclocalization 0.001630.02739&radic&radic S000000534BIK1YCL029CverifiedMicrotubule-associated protein, component of the interfacebetween microtubules and kinetochore, involvedin sister chromatid separation; essential inpolyploid cells but not in haploid or diploidcells; ortholog of mammalian CLIP-170 0.001630.02739&radic&radic&radic S000005752ISU2YOR226CverifiedConserved protein of the mitochondrial matrix, required forsynthesis of mitochondrial and cytosoliciron-sulfur proteins, performs a scaffoldingfunction in mitochondria during Fe/S clusterassembly; isu1 isu2 double mutant is inviable 0.001620.02739&radic S000005686MTR10YOR160WverifiedNuclear import receptor, mediates the nuclear localization ofproteins involved in mRNA-nucleus export 0.00160.02707&radic S000005242CLA4YNL298WverifiedCdc42p activated signal transducing kinase of the PAK(p21-activated kinase) family, involved inseptin ring assembly and cytokinesis; directlyphosphorylates septins Cdc3p and Cdc10p; otheryeast PAK family members are Ste20p and Skm1p 0.001610.02707&radic&radic S000002655VHS1YDR247WverifiedCytoplasmic serine/threonine protein kinase; identified as ahigh-copy suppressor of the synthetic lethalityof a sis2 sit4 double mutant, suggesting a rolein G1/S phase progression; homolog of Sks1p 0.001610.02707&radic&radic S000005163ALG9YNL219CverifiedMannosyltransferase, involved in N-linked glycosylation;catalyzes the transfer of mannose fromDol-P-Man to lipid-linked oligosaccharides;mutation of the human ortholog causes type 1congenital disorders of glycosylation 0.001610.02707&radic&radic&radic S000002776YPR1YDR368Wverified2-methylbutyraldehyde reductase, may be involved in isoleucinecatabolism 0.001610.02707&radic&radic S000004161YLR171WDubiousHypothetical protein 0.001610.02707&radic&radic S000001936SAP155YFR040WverifiedProtein that forms a complex with the Sit4p protein phosphataseand is required for its function; member of afamily of similar proteins including Sap4p,Sap185p, and Sap190p 0.00160.02668&radic S000000378YBR174CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective 0.00160.02668&radic S000005585YOR059CUncharacterizedHypothetical protein 0.00160.02668 S000002464YOS9YDR057WverifiedLectin; soluble lumenal ER protein; member of the OS-9 proteinfamily; similar to mannose-6-phosphatereceptors (MPRs); serves as a receptor thatrecognizes misfolded N-glycosylated proteinsand participates in their targeting to ERAD 0.00160.02668&radic S000004827SCJ1YMR214WverifiedOne of several homologs of bacterial chaperone DnaJ, located inthe ER lumen where it cooperates with Kar2p tomediate maturation of proteins 0.00160.02668&radic S000001611PMU1YKL128CverifiedPutative phosphomutase, contains a region homologous to theactive site of phsophomutases; overexpressionof the gene suppresses the histidine auxotrophyof an ade3 ade16 ade17 triple mutant and thetemperature sensitivity of a tps2 mutant 0.001590.02657&radic&radic&radic S000001019RIM101YHL027WverifiedTranscriptional repressor involved in response to pH and incell wall construction; required for alkalinepH-stimulated haploid invasive growth andsporulation; activated by proteolyticprocessing; similar to A. nidulans PacC 0.001590.02657&radic S000004833ERG8YMR220WverifiedPhosphomevalonate kinase, an essential cytosolic enzyme thatacts in the biosynthesis of isoprenoids andsterols, including ergosterol, from mevalonate 0.001590.02639&radic&radic&radic S000005160RAP1YNL216WverifiedDNA-binding protein involved in either activation or repressionof transcription, depending on binding sitecontext; also binds telomere sequences andplays a role in telomeric position effect(silencing) and telomere structure 0.001590.0261&radic&radic&radic&radic S000003166YIP4YGL198WverifiedProtein that interacts with Rab GTPases; computational analysisof large-scale protein-protein interaction datasuggests a possible role in vesicle-mediatedtransport 0.001590.0261&radic&radic S000002701SSD1YDR293CverifiedProtein with a role in maintenance of cellular integrity,interacts with components of the TOR pathway;ssd1 mutant of a clinical S. cerevisiae straindisplays elevated virulence 0.001590.0261&radic&radic S000001380RHO3YIL118WverifiedNon-essential small GTPase of the Rho/Rac subfamily of Ras-likeproteins involved in the establishment of cellpolarity; GTPase activity positively regulatedby the GTPase activating protein (GAP) Rgd1p 0.001580.02591&radic S000005885HAP5YOR358WverifiedSubunit of the heme-activated, glucose-repressed Hap2/3/4/5CCAAT-binding complex, a transcriptionalactivator and global regulator of respiratorygene expression; required for assembly and DNAbinding activity of the complex 0.001580.02574&radic&radic&radic&radic S000005372HTZ1YOL012CverifiedHistone variant H2AZ, exchanged for histone H2A in nucleosomesby the SWR1 complex; involved intranscriptional regulation through preventionof the spread of silent heterochromatin 0.001570.0251&radic&radic&radic S000003039AFT1YGL071WverifiedTranscription factor involved in iron utilization andhomeostasis; binds the consensus sitePyPuCACCCPu and activates the expression oftarget genes in response to changes in ironavailability 0.001570.0251&radic&radic&radic&radic S000004065RPL10YLR075WverifiedProtein component of the large (60S) ribosomal subunit,responsible for joining the 40S and 60Ssubunits; regulates translation initiation; hassimilarity to rat L10 ribosomal protein and tomembers of the QM gene family 0.001570.0251&radic&radic S000004079ALT1YLR089CuncharacterizedPutative alanine transaminase (glutamic pyruvic transaminase);the authentic, non-tagged protein is detectedin highly purified mitochondria inhigh-throughput studies 0.001570.0251&radic&radic S000004774MSS11YMR164CverifiedTranscription factor involved in regulation of invasive growthand starch degradation; controls the activationof MUC1 and STA2 in response to nutritionalsignals 0.001570.0251&radic&radic S000005638CEX1YOR112WverifiedCytoplasmic component of the nuclear aminoacylation-dependenttRNA export pathway; interacts with nuclearpore component Nup116p; copurifies with tRNAexport receptors Los1p and Msn5p, as well aseIF-1a and the RAN GTPase Gsp1p 0.001560.02503 S000004081YLR091WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR091W is not an esssential gene 0.001560.02477&radic&radic S000000122LSM2YBL026WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.001560.02477&radic&radic&radic S000002828HKR1YDR420WverifiedSerine/threonine rich cell surface protein that contains an EFhand motif; involved in the regulation of cellwall beta-1,3 glucan synthesis and bud siteselection; overexpression confers resistance toHansenula mrakii killer toxin, HM-1 0.001560.02477&radic S000003703ERG20YJL167WverifiedFarnesyl pyrophosphate synthetase, has bothdimethylallyltranstransferase andgeranyltranstransferase activities; catalyzesthe formation of C15 farnesyl pyrophosphateunits for isoprenoid and sterol biosynthesis 0.001560.02477&radic S000000550YCL045CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; interacts with Gal80p; YCL045C is not an essential gene 0.001560.02446&radic&radic S000003308MRPL25YGR076CverifiedMitochondrial ribosomal protein of the large subunit 0.001550.02446&radic&radic S000000397MED8YBR193CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation 0.001550.02446&radic&radic S000000468YPT10YBR264CverifiedGTP binding protein that contains the PEST signal sequencespecific for proteolytic enzymes; may beinvolved in vesicular transport; overexpressionleads to accumulation of Golgi-like cisternaewith budding vesicles 0.001550.02442 S000005756WTM1YOR230WverifiedTranscriptional repressor involved in regulation of meiosis andsilencing; contains WD repeats 0.001550.02429&radic&radic&radic S000005156VID27YNL212WverifiedCytoplasmic protein of unknown function; possibly involved invacuolar protein degradation; not essential forproteasome-dependent degradation offructose-1,6-bisphosphatase (FBPase); nullmutants exhibit normal growth 0.001540.02413&radic&radic S000000018ATS1YAL020CverifiedProtein required, with Elongator complex, Kti11p, and Kti12p,for modification of wobble nucleosides in tRNA;has a potential role in regulatory interactionsbetween microtubules and the cell cycle 0.001540.02392&radic&radic S000004808ICY1YMR195WuncharacterizedProtein of unknown function, required for viability in richmedia of cells lacking mitochondrial DNA 0.001540.02392&radic&radic S000002635SIR4YDR227WverifiedSilent information regulator that, together with SIR2 and SIR3,is involved in assembly of silent chromatindomains at telomeres and the silent mating-typeloci; potentially phosphorylated by Cdc28p;some alleles of SIR4 prolong lifespan 0.001540.02382&radic&radic S000002676MSW1YDR268WverifiedMitochondrial tryptophanyl-tRNA synthetase 0.001530.02372&radic&radic S000002706ATP5YDR298CverifiedSubunit 5 of the stator stalk of mitochondrial F1F0 ATPsynthase, which is a large, evolutionarilyconserved enzyme complex required for ATPsynthesis; homologous to bovine subunit OSCP(oligomycin sensitivity-conferring protein) 0.001530.02372&radic&radic S000002749YDR341CVerifiedCytoplasmic arginyl-tRNA synthetase 0.001530.02372&radic&radic S000005150RTT106YNL206CverifiedProtein with a role in regulation of Ty1 transposition 0.001530.02372&radic&radic&radic&radic S000003412RNR4YGR180CverifiedRibonucleotide-diphosphate reductase (RNR), small subunit; theRNR complex catalyzes the rate-limiting step indNTP synthesis and is regulated by DNAreplication and DNA damage checkpoint pathwaysvia localization of the small subunits 0.001530.02372&radic&radic&radic&radic S000001346SDS3YIL084CverifiedComponent of the Rpd3p/Sin3p deacetylase complex required forits structural integrity and catalyticactivity, involved in transcriptional silencingand required for sporulation; cells defectivein SDS3 display pleiotropic phenotypes 0.001530.02355&radic&radic&radic&radic S000006342MEP3YPR138CverifiedAmmonium permease of high capacity and low affinity; belongs toa ubiquitous family of cytoplasmic membraneproteins that transport only ammonium (NH4+);expression is under the nitrogen cataboliterepression regulation ammonia permease 0.001530.02355&radic&radic S000005385LAG2YOL025WverifiedProtein involved in determination of longevity; LAG2 gene ispreferentially expressed in young cells;overexpression extends the mean and maximumlife span of cells 0.001520.0232&radic&radic S000000128HEK2YBL032WverifiedRNA binding protein with similarity to hnRNP-K that localizesto the cytoplasm and to subtelomeric DNA;required for the proper localization of ASH1mRNA; involved in the regulation of telomereposition effect and telomere length 0.001520.0232&radic&radic S000003050YGL082WUncharacterizedHypothetical protein 0.001520.0232&radic&radic&radic S000006328CTR1YPR124WverifiedHigh-affinity copper transporter of the plasma membrane,mediates nearly all copper uptake under lowcopper conditions; transcriptionally induced atlow copper levels and degraded at high copperlevels 0.001520.0232&radic&radic S000004625MSS1YMR023CverifiedMitochondrial protein, forms a heterodimer complex with Mto1pthat performs the 5-carboxymethylaminomethylmodification of the wobble uridine base inmitochondrial tRNAs; similar to human GTPBP3 0.001520.0232&radic S000003197SHE10YGL228WverifiedPutative glycosylphosphatidylinositol (GPI)-anchored protein ofunknown function; overexpression causes growtharrest 0.001520.0232&radic&radic S000003213RTF1YGL244WverifiedSubunit of the RNA polymerase II-associated Paf1 complex;directly or indirectly regulates DNA-bindingproperties of Spt15p and relative activities ofdifferent TATA elements; involved in telomeremaintenance 0.001520.0232&radic&radic&radic&radic S000005937SWI1YPL016WverifiedSubunit of the SWI/SNF chromatin remodeling complex, whichregulates transcription by remodelingchromosomes; required for transcription of manygenes, including ADH1, ADH2, GAL1, HO, INO1 andSUC2 0.001510.02293&radic&radic&radic S000004071GAL2YLR081WverifiedGalactose permease, required for utilization of galactose; alsoable to transport glucose 0.001520.02293&radic S000004075ARP6YLR085CverifiedActin-related protein that binds nucleosomes; a component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.001510.02293&radic&radic S000000555APA1YCL050CverifiedDiadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4Aphosphorylase), involved in catabolism ofbis(5'-nucleosidyl) tetraphosphates; hassimilarity to Apa2p 0.001520.02293&radic&radic&radic S000003022ERV14YGL054CverifiedProtein localized to COPII-coated vesicles, involved in vesicleformation and incorporation of specificsecretory cargo; required for the delivery ofbud-site selection protein Axl2p to cellsurface; related to Drosophila cornichon 0.001510.02293&radic&radic S000005651CAT5YOR125CverifiedMitochondrial inner membrane protein directly involved inubiquinone biosynthesis, essential for severalother metabolic pathways including respirationand gluconeogenic gene activation 0.001510.02293&radic&radic S000004265SMD2YLR275WverifiedCore Sm protein Sm D2; part of heteroheptameric complex (withSmb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D2 0.001510.02293&radic&radic&radic S000004896RIT1YMR283Cverified2'-O-ribosyl phosphate transferase, modifies the initiatormethionine tRNA at position 64 to distinguishit from elongator methionine tRNA 0.001510.02293&radic S000004866YMR253CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene 0.001490.02226&radic&radic S000002999RPL24AYGL031CverifiedRibosomal protein L30 of the large (60S) ribosomal subunit,nearly identical to Rpl24Bp and has similarityto rat L24 ribosomal protein; not essential fortranslation but may be required for normaltranslation rate 0.00150.02226&radic S000000530AGP1YCL025CverifiedLow-affinity amino acid permease with broad substrate range,involved in uptake of asparagine, glutamine,and other amino acids; expression is regulatedby the SPS plasma membrane amino acid sensorsystem (Ssy1p-Ptr3p-Ssy5p) 0.001490.02226&radic&radic S000005841YOR314WDubiousHypothetical protein 0.00150.02226&radic S000003207CSE1YGL238WverifiedNuclear envelope protein that mediates the nuclear export ofimportin alpha (Srp1p), homolog of metazoan CASprotein, required for accurate chromosomesegregation 0.00150.02226&radic&radic&radic S000003041HSF1YGL073WverifiedTrimeric heat shock transcription factor, activates multiplegenes in response to stresses that includehyperthermia; recognizes variable heat shockelements (HSEs) consisting of inverted NGAANrepeats; posttranslationally regulated 0.001490.02226&radic&radic&radic&radic S000003374BTN2YGR142Wverifiedv-SNARE binding protein that facilitates specific proteinretrieval from a late endosome to the Golgi;modulates arginine uptake, possible role inmediating pH homeostasis between the vacuoleand plasma membrane H(+)-ATPase 0.00150.02226&radic&radic S000000070ADE1YAR015WverifiedN-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR)synthetase, required for 'de novo' purinenucleotide biosynthesis; red pigmentaccumulates in mutant cells deprived of adenine 0.001490.02226&radic&radic S000003842EAF6YJR082CuncharacterizedEsa1p-associated factor, subunit of the NuA4 acetyltransferasecomplex 0.001490.02226&radic S000002725HIM1YDR317WverifiedProtein of unknown function involved in DNA repair 0.001510.02226&radic&radic S000000081SWH1YAR042WverifiedProtein similar to mammalian oxysterol-binding protein;contains ankyrin repeats; localizes to theGolgi and the nucleus-vacuole junction 0.00150.02226&radic&radic S000005996GCR1YPL075WverifiedTranscriptional activator of genes involved in glycolysis;DNA-binding protein that interacts andfunctions with the transcriptional activatorGcr2p 0.001490.02226&radic&radic&radic S000004035STU2YLR045CverifiedMicrotubule-associated protein (MAP) of the XMAP215/Dis1family; regulates microtubule dynamics duringspindle orientation and metaphase chromosomealignment; interacts with spindle pole bodycomponent Spc72p 0.001490.02226&radic&radic S000002754SVF1YDR346CverifiedProtein with a potential role in cell survival pathways,required for the diauxic growth shift;expression in mammalian cells increasessurvival under conditions inducing apoptosis 0.001510.02226&radic&radic&radic S000005649LEO1YOR123CverifiedComponent of the Paf1 complex, which associates with RNApolymerase II and is involved in histonemethylation 0.00150.02226&radic&radic S000002820RRP17YDR412WuncharacterizedProtein required for cell viability 0.00150.02226&radic&radic S000006302YPR098CVerifiedProtein of unknown function, localized to the mitochondrial outer membrane 0.001490.02222 S000005551HST3YOR025WverifiedMember of the Sir2 family of NAD(+)-dependent proteindeacetylases; involved along with Hst4p intelomeric silencing, cell cycle progression,radiation resistance, genomic stability andshort-chain fatty acid metabolism 0.001490.02208&radic&radic&radic S000005731FMP38YOR205CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.001490.02208&radic S000001780SIS2YKR072CverifiedNegative regulatory subunit of the protein phosphatase 1 Ppz1p;involved in ion homeostasis and cell cycleprogression 0.001490.02208&radic&radic S000005784HNT3YOR258WverifiedMember of the third branch of the histidine triad (HIT)superfamily of nucleotide-binding proteins;similar to Aprataxin, a Hint related proteinthat is mutated in individuals with ataxia withoculomotor apraxia 0.001480.02208&radic&radic S000000653PWP2YCR057CverifiedConserved 90S pre-ribosomal component essential for properendonucleolytic cleavage of the 35 S rRNAprecursor at A0, A1, and A2 sites; containseight WD-repeats; PWP2 deletion leads todefects in cell cycle and bud morphogenesis 0.001490.02208&radic S000006144GRE1YPL223CverifiedHydrophilin of unknown function; stress induced (osmotic,ionic, oxidative, heat shock and heavy metals);regulated by the HOG pathway 0.001480.02203&radic&radic S000005590YNG1YOR064CverifiedSubunit of the NuA3 histone acetyltransferase complex thatacetylates histone H3; has similarity to thehuman tumor suppressor ING1 0.001480.02186&radic&radic&radic&radic S000005838HSD1YOR311CverifiedEndoplasmic reticulum (ER)-resident membrane protein,overproduction induces enlargement of ER-likemembrane structures and suppresses atemperature-sensitive sly1 mutation 0.001480.02186&radic&radic S000000439YBR235WUncharacterizedPutative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene 0.001480.02186&radic&radic&radic S000000013NTG1YAL015CverifiedDNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involvedin base excision repair, localizes to thenucleus and mitochondrion 0.001480.02186&radic&radic&radic S000001319YIL057CUncharacterizedHypothetical protein 0.001480.02186&radic&radic S000000066SEN34YAR008WverifiedSubunit of the tRNA splicing endonuclease, which is composed ofSen2p, Sen15p, Sen34p, and Sen54p; Sen34pcontains the active site for tRNA 3' splicesite cleavage and has similarity to Sen2p andto Archaeal tRNA splicing endonuclease 0.001480.02186&radic&radic S000004556RPM2YML091CverifiedProtein component of mitochondrial RNase P, along with themitochondrially-encoded RNA subunit RPM1; RnaseP removes 5' extensions from tRNA precursors;Rpm2p is also involved in maturation of RPM1and in translation of mitochondrial mRNAs 0.001480.02186 S000006029YPL108WUncharacterizedHypothetical protein 0.001480.02186&radic&radic S000005626CRC1YOR100CverifiedMitochondrial inner membrane carnitine transporter, requiredfor carnitine-dependent transport of acetyl-CoAfrom peroxisomes to mitochondria during fattyacid beta-oxidation 0.001480.02182&radic&radic S000005098YCK2YNL154CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck1p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck1p 0.001480.02182&radic&radic&radic S000005825BUD7YOR299WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p, from the Golgi to plasma membrane 0.001480.02182&radic S000001133MSR1YHR091CverifiedMitochondrial arginyl-tRNA synthetase 0.001470.02125&radic&radic S000003288RSC1YGR056WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; containstwo essential bromodomains, a bromo-adjacenthomology (BAH) domain, and an AT hook 0.001460.02125&radic&radic S000002480SNF11YDR073WverifiedSubunit of the SWI/SNF chromatin remodeling complex involved intranscriptional regulation; interacts with ahighly conserved 40-residue sequence of Snf2p 0.001460.02125&radic&radic S000005422PRS5YOL061Wverified5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involvedin nucleotide, histidine, and tryptophanbiosynthesis; one of a five related enzymes,which are active as heteromultimeric complexes 0.001470.02125&radic&radic S000005371PLB3YOL011WverifiedPhospholipase B (lysophospholipase) involved in phospholipidmetabolism; hydrolyzes phosphatidylinositol andphosphatidylserine and displays transacylaseactivity in vitro 0.001470.02125 S000005498YOL138CUncharacterizedHypothetical protein 0.001470.02125 S000005584ASE1YOR058CverifiedMember of a family of microtubule-associated proteins (MAPs)that function at the mitotic spindle midzone;required for spindle elongation; undergoes cellcycle-regulated degradation by anaphasepromoting complex; potential Cdc28p substrate 0.001470.02125&radic&radic&radic S000002579SUP35YDR172WverifiedTranslation termination factor eRF3; altered proteinconformation creates the [PSI(+)] prion, adominant cytoplasmically inherited proteinaggregate that alters translational fidelityand creates a nonsense suppressor phenotype 0.001470.02125&radic&radic S000000170AAR2YBL074CverifiedComponent of the U5 snRNP, required for splicing of U3precursors; originally described as a splicingfactor specifically required for splicingpre-mRNA of the MATa1 cistron 0.001470.02125&radic&radic S000000398SOY1YBR194WuncharacterizedProtein proposed to be associated with the nuclear pore complex 0.001470.02125&radic&radic&radic&radic S000001871BUD27YFL023WverifiedProtein involved in bud-site selection, nutrient signaling, andgene expression controlled by the TOR kinase;diploid mutants display a random buddingpattern instead of the wild-type bipolarpattern 0.001460.02125&radic&radic S000003669MRS3YJL133WverifiedMitochondrial iron transporter of the mitochondrial carrierfamily (MCF), very similar to and functionallyredundant with Mrs4p; functions under low-ironconditions; may transport other cations inaddition to iron 0.001470.02125&radic&radic S000002722RAD34YDR314CverifiedProtein involved in nucleotide excision repair (NER);homologous to RAD4 0.001470.02125&radic&radic S000000388YBR184WUncharacterizedPutative protein of unknown function; YBR184W is not an essential gene 0.001470.02125&radic&radic S000002665SET7YDR257CverifiedNuclear protein that contains a SET-domain, which have beenshown to mediate methyltransferase activity inother proteins 0.001470.02125&radic&radic&radic&radic S000006086SET6YPL165CverifiedProtein of unknown function; deletion heterozygote is sensitiveto compounds that target ergosterolbiosynthesis, may be involved in compoundavailability 0.001470.02125 S000006312RPN7YPR108WverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome,similar to another S. cerevisiae regulatorysubunit, Rpn5p, as well as to mammalianproteasome subunits 0.001460.02097&radic&radic&radic&radic S000000596MRPL32YCR003WverifiedMitochondrial ribosomal protein of the large subunit 0.001460.02097&radic&radic S000004085IOC2YLR095CverifiedMember of a complex (Isw1b) with Isw1p and Ioc4p that exhibitsnucleosome-stimulated ATPase activity and actswithin coding regions to coordinatetranscription elongation with termination andprocessing, contains a PHD finger motif 0.001460.02097&radic&radic&radic&radic S000000732YEA6YEL006WverifiedPutative mitochondrial NAD+ transporter, member of themitochondrial carrier subfamily (see alsoYIA6); has putative human ortholog 0.001460.02097&radic&radic S000002575CDC37YDR168WverifiedEssential Hsp90p co-chaperone; necessary for passage throughthe START phase of the cell cycle 0.001460.02097&radic&radic&radic S000003018TYW3YGL050WuncharacterizedHypothetical protein 0.001460.02097&radic&radic S000005543PET127YOR017WverifiedProtein with a role in mitochondrial RNA stability and/orprocessing, located in the mitochondrialmembrane 0.001450.02097&radic&radic S000003136HUR1YGL168WuncharacterizedProtein required for hydroxyurea resistance; has possible rolesin DNA replication and maintenance of propertelomere length 0.001460.02097 S000002721PIB1YDR313CverifiedRING-type ubiquitin ligase of the endosomal and vacuolarmembranes, bindsphosphatidylinositol(3)-phosphate; contains aFYVE finger domain 0.001460.02097&radic&radic S000001911GSY1YFR015CverifiedGlycogen synthase with similarity to Gsy2p, the more highlyexpressed yeast homolog; expression induced byglucose limitation, nitrogen starvation,environmental stress, and entry into stationaryphase 0.001460.02097&radic S000005176CSL4YNL232WverifiedSubunit of the exosome, which is an essential complex presentin both nucleus and cytoplasm that mediates RNAprocessing and degradation 0.001450.02087&radic&radic&radic S000002315YDL156WUncharacterizedHypothetical protein 0.001450.02087&radic S000001910CMK1YFR014CverifiedCalmodulin-dependent protein kinase; may play a role in stressresponse, many CA++/calmodulan dependentphosphorylation substrates demonstrated invitro, amino acid sequence similar to Cmk2p andmammalian Cam Kinase II 0.001450.02087&radic&radic S000005839RPL20BYOR312CverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl20Ap and has similarity to ratL18a ribosomal protein 0.001450.02087&radic S000005213BSC4YNL269WverifiedProtein of unknown function, ORF exhibits genomic organizationcompatible with a translationalreadthrough-dependent mode of expression;readthrough is increased upon depletion ofSup35p 0.001450.02087&radic&radic S000001001YAP3YHL009CverifiedBasic leucine zipper (bZIP) transcription factor 0.001450.02087&radic&radic&radic S000003188YGL220WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus 0.001450.02083&radic&radic&radic S000003158CDC55YGL190CverifiedNon-essential regulatory subunit B of protein phosphatase 2A;has multiple roles in mitosis and proteinbiosynthesis; found in the nucleus of mostcells but also at the bud neck (large-buddedcells) and at the bud tip (small-budded cells) 0.001450.02057 S000002589SAS4YDR181CverifiedSubunit of the SAS complex (Sas2p, Sas4p, Sas5p), whichacetylates free histones and nucleosomes andregulates transcriptional silencing; requiredfor the HAT activity of Sas2p 0.001440.02057&radic&radic S000000598CIT2YCR005CverifiedCitrate synthase, catalyzes the condensation of acetyl coenzymeA and oxaloacetate to form citrate, peroxisomalisozyme involved in glyoxylate cycle;expression is controlled by Rtg1p and Rtg2ptranscription factors 0.001440.02057&radic&radic S000002599HST4YDR191WverifiedMember of the Sir2 family of NAD(+)-dependent proteindeacetylases; involved along with Hst3p insilencing at telomeres, cell cycle progression,radiation resistance, genomic stability andshort-chain fatty acid metabolism 0.001440.02057&radic&radic&radic&radic S000003451YGR219WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C 0.001450.02057&radic&radic&radic S000005985CWC27YPL064CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to S. pombeCwf27p 0.001450.02057&radic&radic&radic&radic S000000125YBL029WUncharacterizedHypothetical protein 0.001450.02057&radic&radic S000000514STP22YCL008CverifiedComponent of the ESCRT-I complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; homologous to the mouse and humanTsg101 tumor susceptibility gene; mutantsexhibit a Class E Vps phenotype 0.001440.02046&radic&radic S000000031POP5YAL033WverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.001440.02046&radic&radic&radic S000001312PCL7YIL050WverifiedPho85p cyclin of the Pho80p subfamily, forms a functionalkinase complex with Pho85p which phosphorylatesMmr1p and is regulated by Pho81p; involved inglycogen metabolism, expression is cell-cycleregulated 0.001440.02046&radic S000003335NOP7YGR103WverifiedNucleolar protein involved in rRNA processing and 60S ribosomalsubunit biogenesis; constituent of severaldifferent pre-ribosomal particles; required forexit from G0 and the initiation ofcell proliferation 0.001440.02031&radic&radic&radic S000004109SRN2YLR119WverifiedComponent of the ESCRT-I complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; suppressor of rna1-1 mutation;may be involved in RNA export from nucleus 0.001440.02031 S000005670ELG1YOR144CverifiedProtein required for S phase progression and telomerehomeostasis, forms an alternative replicationfactor C complex important for DNA replicationand genome integrity; involved in homologousrecombination-mediated DNA repair 0.001440.02031&radic S000003019MST27YGL051WverifiedPutative integral membrane protein, involved in vesicleformation; forms complex with Mst28p; member ofDUP240 gene family; binds COPI and COPIIvesicles 0.001440.02031&radic S000000803MNN1YER001WverifiedAlpha-1,3-mannosyltransferase, integral membrane glycoproteinof the Golgi complex, required for addition ofalpha1,3-mannose linkages to N-linked andO-linked oligosaccharides, one of five S.cerevisiae proteins of the MNN1 family 0.001440.02031&radic&radic S000002844PPZ2YDR436WverifiedSerine/threonine protein phosphatase Z, isoform of Ppz1p;involved in regulation of potassium transport,which affects osmotic stability, cell cycleprogression, and halotolerance 0.001440.02031&radic S000000036CDC19YAL038WverifiedPyruvate kinase, functions as a homotetramer in glycolysis toconvert phosphoenolpyruvate to pyruvate, theinput for aerobic (TCA cycle) or anaerobic(glucose fermentation) respiration 0.001430.02013&radic S000005739SAS5YOR213CverifiedSubunit of the SAS complex (Sas2p, Sas4p, Sas5p), whichacetylates free histones and nucleosomes andregulates transcriptional silencing; stimulatesSas2p HAT activity 0.001440.02013&radic&radic&radic&radic S000002729ASP1YDR321WverifiedCytosolic L-asparaginase, involved in asparagine catabolism 0.001430.02013&radic&radic S000001935YFR039CUncharacterizedPutative protein of unknown function; YFR039C is not an essential gene 0.001430.02013&radic S000002459DBF4YDR052CverifiedRegulatory subunit of Cdc7p-Dbf4p kinase complex, required forCdc7p kinase activity and initiation of DNAreplication; phosphorylates the Mcm2-7 familyof proteins; cell cycle regulated 0.001430.02013&radic&radic&radic S000002775YDR367WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 0.001430.02013&radic&radic S000004616BUD22YMR014WverifiedProtein involved in bud-site selection; diploid mutants displaya random budding pattern instead of thewild-type bipolar pattern 0.001430.02013&radic&radic&radic S000005573STD1YOR047CverifiedProtein involved in control of glucose-regulated geneexpression; interacts with protein kinaseSnf1p, glucose sensors Snf3p and Rgt2p, andTATA-binding protein Spt15p; acts as aregulator of the transcription factor Rgt1p 0.001440.02013 S000001897LOC1YFR001WverifiedNuclear protein involved in asymmetric localization of ASH1mRNA; binds double-stranded RNA in vitro;constituent of 66S pre-ribosomal particles 0.001430.02013&radic&radic&radic&radic S000002248RAM1YDL090CverifiedBeta subunit of the CAAX farnesyltransferase (FTase) thatprenylates the a-factor mating pheromone andRas proteins; required for the membranelocalization of Ras proteins and a-factor;homolog of the mammalian FTase beta subunit 0.001430.02013&radic&radic S000005605ATX2YOR079CverifiedGolgi membrane protein involved in manganese homeostasis;overproduction suppresses the sod1 (copper,zinc superoxide dismutase) null mutation 0.001430.02013&radic S000005816SNF2YOR290CverifiedCatalytic subunit of the SWI/SNF chromatin remodeling complexinvolved in transcriptional regulation;contains DNA-stimulated ATPase activity;functions interdependently in transcriptionalactivation with Snf5p and Snf6p 0.001430.02&radic&radic S000002970ERP6YGL002WverifiedProtein with similarity to Emp24p and Erv25p, member of the p24family involved in ER to Golgi transport; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 0.001430.02&radic S000001934IRC5YFR038WuncharacterizedPutative ATPase containing the DEAD/H helicase-related sequencemotif; null mutant displays increased levels ofspontaneous Rad52 foci 0.001420.01983&radic&radic&radic S000004202TUB4YLR212CverifiedGamma-tubulin, involved in nucleating microtubules from boththe cytoplasmic and nuclear faces of thespindle pole body 0.001420.01983&radic S000002661MET32YDR253CverifiedZinc-finger DNA-binding protein, involved in regulatingexpression of the methionine biosyntheticgenes, similar to Met31p 0.001420.01983&radic&radic&radic&radic S000004826CEF1YMR213WverifiedEssential splicing factor; associated with Prp19p and thespliceosome, contains an N-terminal c-Myb DNAbinding motif necessary for cell viability butnot for Prp19p association, evolutionarilyconserved and homologous to S. pombe Cdc5p 0.001420.01969&radic&radic&radic S000004840TAF7YMR227CverifiedTFIID subunit (67 kDa), involved in RNA polymerase IItranscription initiation 0.001420.01969&radic&radic&radic S000004985YNL040WUncharacterizedPutative protein of unknown function with strong similarity to alanyl-tRNA synthases from Eubacteria; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene 0.001420.01969 S000005678YOR152CUncharacterizedHypothetical protein 0.001420.01969&radic&radic S000000049FLC2YAL053WverifiedPutative FAD transporter; required for uptake of FAD intoendoplasmic reticulum; involved in cell wallmaintenance 0.001420.01969&radic&radic S000004511GSF2YML048WverifiedER localized integral membrane protein that may promotesecretion of certain hexose transporters,including Gal2p; involved in glucose-dependentrepression 0.001420.01969 S000001770TFA2YKR062WverifiedTFIIE small subunit, involved in RNA polymerase IItranscription initiation 0.001420.01969&radic&radic&radic&radic S000003984MMP1YLL061WverifiedHigh-affinity S-methylmethionine permease, required forutilization of S-methylmethionine as a sulfursource; has similarity to S-adenosylmethioninepermease Sam3p 0.001420.01969&radic S000005201SIP3YNL257CverifiedProtein that activates transcription through interaction withDNA-bound Snf1p, C-terminal region has aputative leucine zipper motif; potential Cdc28psubstrate 0.001420.01942 S000004889DSK2YMR276WverifiedNuclear-enriched ubiquitin-like polyubiquitin-binding protein,required for spindle pole body (SPB)duplication and for transit through the G2/Mphase of the cell cycle, involved inproteolysis, interacts with the proteasome 0.001420.01942&radic&radic&radic&radic S000004690YMR085WUncharacterizedHypothetical protein 0.001410.01942 S000006183FUM1YPL262WverifiedFumarase, converts fumaric acid to L-malic acid in the TCAcycle; cytosolic and mitochondrial localizationdetermined by the N-terminal mitochondrialtargeting sequence and protein conformation 0.001420.01942&radic&radic S000002552TAF12YDR145WverifiedSubunit (61/68 kDa) of TFIID and SAGA complexes, involved inRNA polymerase II transcription initiation andin chromatin modification, similar to histoneH2A 0.001410.01942&radic&radic&radic&radic S000005771DGA1YOR245CverifiedDiacylglycerol acyltransferase, catalyzes the terminal step oftriacylglycerol (TAG) formation, acylatesdiacylglycerol using acyl-CoA as an acyl donor,localized to lipid particles 0.001420.01942&radic&radic S000001625MRP8YKL142WverifiedPutative mitochondrial ribosomal protein, has similarity to E.coli ribosomal protein S2 0.001420.01942&radic&radic S000002744YDR336WUncharacterizedPutative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene 0.001420.01942&radic&radic S000002574TAF10YDR167WverifiedSubunit (145 kDa) of TFIID and SAGA complexes, involved in RNApolymerase II transcription initiation and inchromatin modification 0.001410.01942&radic&radic&radic S000001675ACP1YKL192CverifiedMitochondrial matrix acyl carrier protein, involved inbiosynthesis of octanoate, which is a precursorto lipoic acid; activated byphosphopantetheinylation catalyzed by Ppt2p 0.001410.01936&radic&radic S000002433YDR026CUncharacterizedPutative transcription factor; Myb-like DNA-binding protein that may bind to the Ter region of rDNA; interacts physically with Fob1p, although this interaction is not involved in replication fork arrest 0.001410.01936 S000001778YKR070WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion 0.001410.01936&radic S000003344SHY1YGR112WverifiedMitochondrial inner membrane protein required for normalrespiration, possible chaperone involved inassembly of cytochrome c oxidase; similar toSURF1 from mammals, chickens, and D.melanogaster 0.001410.01936&radic&radic S000001931YFR035CUncharacterizedHypothetical protein 0.001410.01936&radic S000001887BLM10YFL007WverifiedProteosome activator subunit; found in association with coreparticles, with and without the 19S regulatoryparticle; required for resistance to bleomycin,may be involved in protecting against oxidativedamage; similar to mammalian PA200 0.001410.01936&radic&radic&radic S000003058LIF1YGL090WverifiedProtein involved in DNA double-strand break repair; physicallyinteracts with DNA ligase 4 (Lig4p); homologousto mammalian XRCC4 protein 0.001410.01936&radic&radic&radic S000006173YAH1YPL252CverifiedFerredoxin of the mitochondrial matrix required for formationof cellular iron-sulfur proteins; involved inheme A biosynthesis; homologous to humanadrenodoxin 0.00140.01883&radic S000000007SPO7YAL009WverifiedIntegral nuclear/ER membrane protein of unknown function,required for normal nuclear envelope morphologyand sporulation 0.001410.01883&radic&radic S000003521MAL11YGR289CverifiedMaltose permease, inducible high-affinity maltose transporter(alpha-glucoside transporter); encoded in theMAL1 complex locus; member of the 12transmembrane domain superfamily of sugartransporters 0.00140.01883 S000002490RRP8YDR083WverifiedNucleolar protein involved in rRNA processing, pre-rRNAcleavage at site A2; also involved in telomeremaintenance; mutation is synthetically lethalwith a gar1 mutation 0.001410.01883&radic&radic&radic S000005968SGF11YPL047WverifiedIntegral subunit of SAGA histone acetyltransferase complex,regulates transcription of a subset ofSAGA-regulated genes, required for the Ubp8passociation with SAGA and for H2Bdeubiquitylation 0.00140.01883&radic S000000457SRB6YBR253WverifiedEssentital subunit of the RNA polymerase II mediator complex;associates with core polymerase subunits toform the RNA polymerase II holoenzyme;essential for transcriptional regulation 0.00140.01883&radic&radic&radic S000004842RRP5YMR229CverifiedProtein required for the synthesis of both 18S and 5.8S rRNA;C-terminal region is crucial for the formationof 18S rRNA and N-terminal region is requiredfor the 5.8S rRNA; component of small ribosomalsubunit (SSU) processosome 0.00140.01883&radic&radic&radic S000005779NAT5YOR253WverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.001410.01883 S000001107RRP3YHR065CverifiedProtein involved in rRNA processing; required for maturation ofthe 35S primary transcript of pre-rRNA and forcleavage leading to mature 18S rRNA; homologousto eIF-4a, which is a DEAD box RNA-dependentATPase with helicase activity 0.00140.01883&radic&radic&radic S000002692DPP1YDR284CverifiedDiacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulatedvacuolar membrane-associated lipid phosphatase,dephosphorylates DGPP to phosphatidate (PA) andPi, then PA to diacylglycerol; involved inlipid signaling and cell metabolism 0.001410.01883&radic&radic S000004885SCS7YMR272CverifiedSphingolipid alpha-hydroxylase, functions in thealpha-hydroxylation of sphingolipid-associatedvery long chain fatty acids, has bothcytochrome b5-like and hydroxylase/desaturasedomains, not essential for growth 0.001410.01883&radic&radic S000001125SAM35YHR083WverifiedEssential component of the sorting and assembly machinery (SAMcomplex or TOB complex) of the mitochondrialouter membrane, which binds precursors ofbeta-barrel proteins and facilitates theirinsertion into the outer membrane 0.001410.01883&radic&radic S000000881YER079WUncharacterizedHypothetical protein 0.001410.01883&radic&radic S000000804NOP16YER002WverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis 0.00140.01883&radic&radic&radic&radic S000003203ADE5,7YGL234WverifiedBifunctional enzyme of the 'de novo' purine nucleotidebiosynthetic pathway, contains aminoimidazoleribotide synthetase and glycinamide ribotidesynthetase activities 0.001410.01883 S000002134FUN19YAL034CverifiedNon-essential protein of unknown function 0.00140.01883&radic S000002142PAU8YAL068CuncharacterizedHypothetical protein 0.00140.01883 S000005577YOR051CUncharacterizedNuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts 0.001390.01872&radic&radic S000001930PHO4YFR034CverifiedBasic helix-loop-helix (bHLH) transcription factor; bindscooperatively with Pho2p to the PHO5 promoter;function is regulated by phosphorylation atmultiple sites and by phosphate availability 0.001390.01872&radic&radic S000001446YIR007WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene 0.001390.01872 S000006125HRR25YPL204WverifiedProtein kinase involved in regulating diverse events includingvesicular trafficking, gene expression, DNArepair, and chromosome segregation; binds theCTD of RNA pol II; homolog of mammalian caseinkinase 1delta (CK1delta) 0.001390.01872&radic&radic&radic&radic S000007253MBF1YOR298C-AverifiedTranscriptional coactivator that bridges the DNA-binding regionof Gcn4p and TATA-binding protein Spt15p;suppressor of frameshift mutations 0.001390.01872 S000003397MRPS35YGR165WverifiedMitochondrial ribosomal protein of the small subunit 0.001390.0187&radic&radic S000006314RPC40YPR110CverifiedRNA polymerase subunit, common to RNA polymerase I and III 0.001390.0185&radic&radic&radic&radic S000006373JIP5YPR169WuncharacterizedNucleolar protein of unknown function, exhibits a physicalinteraction with Bre1p 0.001390.0185&radic&radic&radic&radic S000005208PDR17YNL264CverifiedPhosphatidylinositol transfer protein (PITP), downregulatesPlb1p-mediated turnover of phosphatidylcholine,found in the cytosol and microsomes, homologousto Pdr16p, deletion affects phospholipidcomposition 0.001390.0185&radic&radic S000002736SKP1YDR328CverifiedEvolutionarily conserved kinetochore protein that is part ofmultiple protein complexes, including the SCFubiquitin ligase complex, the CBF3 complex thatbinds centromeric DNA, and the RAVE complexthat regulates assembly of the V-ATPase 0.001390.0185&radic&radic&radic S000005792PNT1YOR266WverifiedMitochondrial inner membrane protein involved in export ofproteins from the mitochondrial matrix;overexpression of PNT1 confers resistance tothe anti-Pneumocystis carinii drug pentamidine,and deletion confers increased sensitivity 0.001390.0185 S000005609WHI5YOR083WverifiedRepressor of G1 transcription that binds to SCB binding factor(SBF) at SCB target promoters in early G1;phosphorylation of Whi5p by the CDK,Cln3p/Cdc28p relieves repression and promoterbinding by Whi5; periodically expressed in G1 0.001390.0185&radic&radic&radic S000005667ARP8YOR141CverifiedNuclear actin-related protein involved in chromatin remodeling,component of chromatin-remodeling enzymecomplexes 0.001390.0185&radic&radic&radic S000004120ZRT2YLR130CverifiedLow-affinity zinc transporter of the plasma membrane;transcription is induced under low-zincconditions by the Zap1p transcription factor 0.001390.0185 S000004391BDF1YLR399CverifiedProtein involved in transcription initiation at TATA-containingpromoters; associates with the basaltranscription factor TFIID; contains twobromodomains; corresponds to the C-terminalregion of mammalian TAF1; redundant with Bdf2p 0.001390.0185&radic&radic S000001077YHR035WUncharacterizedPutative protein of unknown function; not an essential gene 0.001390.0185&radic&radic S000002769BCP1YDR361CverifiedEssential protein involved in nuclear export of Mss4p, which isa lipid kinase that generatesphosphatidylinositol 4,5-biphosphate and playsa role in actin cytoskeleton organization andvesicular transport 0.001380.01838&radic&radic&radic S000004608PLB2YMR006CverifiedPhospholipase B (lysophospholipase) involved in phospholipidmetabolism; displays transacylase activity invitro; overproduction confers resistance tolysophosphatidylcholine 0.001380.01838&radic&radic S000004734SAS2YMR127CverifiedHistone acetyltransferase (HAT) catalytic subunit of the SAScomplex (Sas2p-Sas4p-Sas5p), which acetylatesfree histones and nucleosomes and regulatestranscriptional silencing; member of theMYSTacetyltransferase family 0.001380.01838&radic&radic S000000595CDC10YCR002CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the membrane, and they comprisethe 10nm filaments seen with EM 0.001380.01838&radic&radic S000005644YOR118WUncharacterizedHypothetical protein 0.001380.01838&radic&radic S000005783CDC31YOR257WverifiedComponent of the spindle pole body (SPB) half-bridge, requiredfor SPB duplication in mitosis and meiosis II;homolog of mammalian centrin; interacts withKar1p 0.001380.01838&radic&radic&radic S000005386MIM1YOL026CverifiedMitochondrial outer membrane protein, required for assembly ofthe translocase of the outer membrane (TOM)complex and thereby for mitochondrial proteinimport; N terminus is exposed to the cytosol:transmembrane segment is highly conserved 0.001380.01828&radic S000003591YJL055WUncharacterizedPutative protein of unknown function; deletion mutants are sensitive to purine base analogs, 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA) and are AHA-hypermutable 0.001380.01828&radic S000000769YEL043WUncharacterizedPredicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; contains fibronectin type III domain fold 0.001380.01828&radic&radic S000000758MCM3YEL032WverifiedProtein involved in DNA replication; component of the Mcm2-7hexameric complex that binds chromatin as apart of the pre-replicative complex 0.001380.01828&radic&radic S000003165MDS3YGL197WverifiedProtein with an N-terminal kelch-like domain, putative negativeregulator of early meiotic gene expression;required, with Pmd1p, for growth under alkalineconditions 0.001380.01828&radic&radic S000005481RPS19AYOL121CverifiedProtein component of the small (40S) ribosomal subunit,required for assembly and maturation of pre-40S particles; mutations in human RPS19 areassociated with Diamond Blackfan anemia; nearlyidentical to Rps19Bp 0.001380.01828 S000005719PEX27YOR193WverifiedPeripheral peroxisomal membrane protein involved in controllingperoxisome size and number, interacts withhomologous protein Pex25p 0.001380.01828&radic S000000773YEL047CVerifiedFumurate ReDuctase Soluble 0.001380.01823&radic&radic&radic S000004044OSW2YLR054CuncharacterizedProtein of unknown function proposed to be involved in theassembly of the spore wall 0.001380.01823&radic&radic S000002696NSE3YDR288WuncharacterizedEssential subunit of the Mms21-Smc5-Smc6 complex; protein ofunknown function; required for DNA repair andgrowth 0.001380.01823&radic&radic&radic S000004954IDP3YNL009WverifiedPeroxisomal NADP-dependent isocitrate dehydrogenase, catalyzesoxidation of isocitrate to alpha-ketoglutaratewith the formation of NADP(H+), required forgrowth on unsaturated fatty acids 0.001380.01823 S000000160PRX1YBL064CverifiedMitochondrial peroxiredoxin (1-Cys Prx) with thioredoxinperoxidase activity, has a role in reduction ofhydroperoxides; induced during respiratorygrowth and under conditions of oxidative stress 0.001380.01823&radic&radic&radic S000002681DON1YDR273WverifiedMeiosis-specific component of the spindle pole body, part ofthe leading edge protein (LEP) coat, forms aring-like structure at the leading edge of theprospore membrane during meiosis II 0.001380.01823&radic&radic S000005696YOR170WDubiousHypothetical protein 0.001380.01819&radic S000004675TVP18YMR071CverifiedIntegral membrane protein localized to late Golgi vesiclesalong with the v-SNARE Tlg2p 0.001380.01819 S000003985MHT1YLL062CverifiedS-methylmethionine-homocysteine methyltransferase, functionsalong with Sam4p in the conversion ofS-adenosylmethionine (AdoMet) to methionine tocontrol the methionine/AdoMet ratio 0.001370.01814&radic S000002699HRQ1YDR291WuncharacterizedPutative DNA helicase 0.001370.01814&radic&radic&radic S000002629GTB1YDR221WverifiedGlucosidase II beta subunit, forms a complex with alpha subunitRot2p, involved in removal of two glucoseresidues from N-linked glycans duringglycoprotein biogenesis in the ER 0.001380.01814&radic&radic S000003417TYS1YGR185CverifiedCytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNAsynthetase that aminoacylates tRNA(Tyr),required for cytoplasmic protein synthesis,interacts with positions 34 and 35 of theanticodon of tRNATyr 0.001370.01814&radic&radic S000004159YLR169WDubiousHypothetical protein 0.001370.01812&radic S000002625RAD9YDR217CverifiedDNA damage-dependent checkpoint protein, required forcell-cycle arrest in G1/S, intra-S, and G2/M;transmits checkpoint signal by activatingRad53p and Chk1p; hyperphosphorylated by Mec1pand Tel1p; potential Cdc28p substrate 0.001370.01803&radic&radic&radic