Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0031202 "RNA splicing factor activity, transesterification mechanism"

GO branch: MF
Number of currently annotated genes: 51

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest TAP:161Harbison:IME1_H2O2HiKumar:nucleusHuh:nucleusHarbison:RCO1_YPDTAP:155Harbison:SFL1_YPDHarbison:DAL80_YPDHarbison:REB1_YPDIPR001163 S000000122LSM2YBL026WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA &radic0.574980.95823&radic&radic&radic&radic&radic S000003306SMD1YGR074WverifiedCore Sm protein Sm D1; part of heteroheptameric complex (withSmb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D1 &radic0.618570.95823&radic&radic&radic&radic&radic&radic S000006386SMX3YPR182WverifiedCore Sm protein Sm F; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm F &radic0.629910.95823&radic&radic&radic&radic S000003660LSM1YJL124CverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)involved in degradation of cytoplasmic mRNAs 0.663810.95823&radic&radic&radic S000004137SMD3YLR147CverifiedCore Sm protein Sm D3; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D3 &radic0.657520.95823&radic&radic&radic S000000831SMB1YER029CverifiedCore Sm protein Sm B; part of heteroheptameric complex (withSmd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm B and Sm B' &radic0.628810.95823&radic&radic&radic&radic S000004265SMD2YLR275WverifiedCore Sm protein Sm D2; part of heteroheptameric complex (withSmb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D2 &radic0.66650.95823&radic&radic&radic&radic&radic&radic S000002965SMX2YFL017W-AverifiedCore Sm protein Sm G; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm G &radic0.615810.95823&radic&radic&radic S000006434LSM3YLR438C-AverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.557780.95823&radic&radic S000005685SME1YOR159CverifiedCore Sm protein Sm E; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm E &radic0.629430.95823&radic&radic&radic&radic S000000914LSM4YER112WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA &radic0.641170.95823&radic&radic&radic&radic S000005091LSM7YNL147WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA &radic0.525330.95312&radic&radic&radic&radic S000000948LSM5YER146WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA &radic0.514680.95157&radic&radic&radic&radic S000002786LSM6YDR378CverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA &radic0.515830.95157&radic&radic&radic&radic S000004107CLF1YLR117CverifiedEssential splicesome assembly factor; contains multipletetratricopeptide repeat (TPR) protein-bindingmotifs and interacts specifically with manyspliceosome components, may serve as a scaffoldduring splicesome assembly 0.37020.93055&radic&radic&radic S000003783LSM8YJR022WverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)that is part of spliceosomal U6 snRNP and isalso implicated in processing of pre-tRNA,pre-snoRNA, and pre-rRNA &radic0.372370.93055&radic&radic&radic&radic&radic&radic S000006382PRP4YPR178WverifiedSplicing factor, component of the U4/U6-U5 snRNP complex &radic0.341380.91709&radic&radic&radic&radic&radic&radic S000003323PRP31YGR091WverifiedSplicing factor, component of the U4/U6-U5 snRNP complex &radic0.328360.90808&radic&radic&radic&radic&radic&radic S000001656SNU114YKL173WverifiedGTPase component of U5 snRNP involved in mRNA splicing viaspliceosome; binds directly to U5 snRNA;proposed to be involved in conformationalchanges of the spliceosome; similarity toribosomal translocation factor EF-2 &radic0.326870.90486&radic&radic&radic&radic&radic S000002824SYF1YDR416WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression; similar to Drosophila crooked neckprotein 0.32640.90476&radic&radic&radic&radic&radic S000006286DIB1YPR082Cverified17-kDa component of the U4/U6aU5 tri-snRNP, plays an essentialrole in pre-mRNA splicing, orthologue of hDIM1,the human U5-specific 15-kDa protein &radic0.317650.90117&radic&radic&radic S000005835SNU66YOR308CverifiedComponent of the U4/U6.U5 snRNP complex involved in pre-mRNAsplicing via spliceosome; has homology to humanSART-1 and to an S. pombe protein; snu66 nullmutation confers cold-sensitivity but is notlethal at normal growth temperatures &radic0.305190.89781&radic&radic&radic&radic S000004901HSH155YMR288WverifiedU2-snRNP associated splicing factor that forms extensiveassociations with the branch site-3' splicesite-3' exon region upon prespliceosomeformation; similarity to the mammalian U2snRNP-associated splicing factor SAP155 0.296640.88941&radic&radic&radic&radic&radic&radic S000003959PRP19YLL036CverifiedSplicing factor associated with the spliceosome; contains aU-box, a motif found in a class of ubiquitinligases &radic0.29570.88823&radic&radic&radic&radic&radic S000006072PRP46YPL151CverifiedSplicing factor that is found in the Cef1p subcomplex of thespliceosome 0.294810.8876&radic&radic&radic S000000614MAK31YCR020C-AverifiedNon-catalytic subunit of N-terminal acetyltransferase of theNatC type; required for replication of dsRNAvirus; member of the Sm protein family 0.264920.87937&radic&radic S000002201PRP11YDL043CverifiedSubunit of the SF3a splicing factor complex, required forspliceosome assembly 0.2560.87682&radic&radic&radic&radic S000005294PRP2YNR011CverifiedRNA-dependent ATPase in the DEAH-box family, required foractivation of the spliceosome before the firsttransesterification step in RNA splicing &radic0.254140.87656&radic&radic&radic S000003739PRP21YJL203WverifiedSubunit of the SF3a splicing factor complex, required forspliceosome assembly 0.253660.87618&radic&radic&radic&radic&radic&radic&radic S000002319DHH1YDL160CverifiedCytoplasmic DExD/H-box helicase, stimulates mRNA decapping,coordinates distinct steps in mRNA function anddecay, interacts with both the decapping anddeadenylase complexes, may have a role in mRNAexport and translation 0.253370.87549&radic&radic&radic S000004732STO1YMR125WverifiedLarge subunit of the nuclear mRNA cap-binding protein complex,interacts with Npl3p to carry nuclear poly(A)+mRNA to cytoplasm; also involved in nuclearmRNA degradation and telomere maintenance;orthologous to mammalian CBP80 0.249980.87248&radic&radic&radic&radic&radic&radic S000006305SNT309YPR101WverifiedComponent of NineTeen complex (NTC) containing Prp19p involvedin mRNA splicing, interacts physically andgenetically with Prp19p &radic0.248420.87098&radic&radic&radic&radic&radic&radic S000003307PRP38YGR075CverifiedUnique component of the U4/U6.U5 tri-snRNP particle,dispensable for spliceosome assembly, butrequired for conformational changes which leadto catalytic activation of the spliceosome &radic0.248260.87045&radic&radic&radic&radic S000002881PRP3YDR473CverifiedSplicing factor, component of the U4/U6-U5 snRNP complex &radic0.246610.86866&radic&radic&radic&radic&radic&radic S000000269ECM2YBR065CverifiedPre-mRNA splicing factor, facilitates the cooperative formationof U2/U6 helix II in association with stem IIin the spliceosome, function may be regulatedby Slu7p 0.242190.86522&radic&radic&radic&radic S000001323SNP1YIL061CverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; may interact with poly(A)polymerase to regulate polyadenylation; homologof human U1 70K protein 0.240740.86404&radic&radic&radic&radic S000002368CWC2YDL209CverifiedProtein involved in pre-mRNA splicing, component of a complexcontaining Cef1p; interacts with Prp19p;contains an RNA recognition motif; hassimilarity to S. pombe Cwf2p 0.240230.86295&radic&radic&radic&radic S000001794PRP16YKR086WverifiedRNA helicase in the DEAH-box family involved in the secondcatalytic step of splicing, exhibitsATP-dependent RNA unwinding activity &radic0.237090.86011&radic S000004826CEF1YMR213WverifiedEssential splicing factor; associated with Prp19p and thespliceosome, contains an N-terminal c-Myb DNAbinding motif necessary for cell viability butnot for Prp19p association, evolutionarilyconserved and homologous to S. pombe Cdc5p &radic0.234570.85962&radic&radic&radic&radic&radic S000000815PRP22YER013WverifiedDEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase,associates with lariat intermediates before thesecond catalytic step of splicing; mediatesATP-dependent mRNA release from the spliceosomeand unwinds RNA duplexes &radic0.229880.85554&radic&radic&radic&radic&radic S000004513RSE1YML049CverifiedProtein involved in pre-mRNA splicing; component of thepre-spliceosome; associates with U2 snRNA;involved in ER to Golgi transport 0.228220.85434&radic&radic S000000259PRP6YBR055CverifiedSplicing factor, component of the U4/U6-U5 snRNP complex &radic0.226650.85273&radic&radic S000004416SPP382YLR424WverifiedEssential protein that forms a dimer with Ntr2p; also forms atrimer, with Ntr2p and Prp43p, that is involvedin spliceosome disassembly; found also in amultisubunit complex with the splicing factorClf1p; suppressor of prp38-1 mutation 0.224850.85155&radic&radic&radic&radic&radic S000004853CUS1YMR240CverifiedProtein required for assembly of U2 snRNP into the spliceosome,forms a complex with Hsh49p and Hsh155p 0.21880.84622&radic&radic S000001448MSL1YIR009WverifiedU2B component of U2 snRNP, involved in splicing, binds the U2snRNA stem-loop IV in vitro; does not containthe conserved C-terminal RNA binding domainfound in other family members 0.215370.84177&radic&radic S000003088PRP43YGL120CverifiedRNA helicase in the DEAH-box family, functions in both RNApolymerase I and polymerase II transcriptmetabolism, involved in release of thelariat-intron from the spliceosome &radic0.213450.84009&radic&radic&radic S000006134LEA1YPL213WverifiedComponent of U2 snRNP; disruption causes reduced U2 snRNPlevels; physically interacts with Msl1p;invovled in telomere maintenance; putativehomolog of human U2A' snRNP protein &radic0.213210.84009&radic&radic&radic S000000659BUD31YCR063WverifiedProtein involved in bud-site selection; analysis of integratedhigh-throughput datasets predicts aninvolvement in RNA splicing; diploid mutantsdisplay a random budding pattern instead of thewild-type bipolar pattern 0.210680.83694&radic&radic&radic S000002367NHP2YDL208WverifiedNuclear protein related to mammalian high mobility group (HMG)proteins, essential for function of H/ACA-typesnoRNPs, which are involved in 18S rRNAprocessing 0.206510.83387&radic&radic S000000030PRP45YAL032CverifiedProtein required for pre-mRNA splicing; associates with thespliceosome and interacts with splicing factorsPrp22p and Prp46p; orthologous to humantranscriptional coactivator SKIP and canactivate transcription of a reporter gene 0.205680.83206&radic&radic S000002256SNU23YDL098CverifiedComponent of U4/U6.U5 snRNP involved in mRNA splicing viaspliceosome &radic0.203570.83087&radic&radic&radic&radic&radic S000000323MUD1YBR119WverifiedU1 snRNP A protein, homolog of human U1-A; involved in nuclearmRNA splicing 0.198630.82495&radic&radic&radic&radic S000002188PRP9YDL030WverifiedSubunit of the SF3a splicing factor complex, required forspliceosome assembly; acts after the formationof the U1 snRNP-pre-mRNA complex 0.198330.82248&radic&radic&radic&radic S000003811ISY1YJR050WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing, auxiliary splicing factor that maymodulate Syf1p activity and help optimizesplicing; isy1 syf2 double mutation activatesthe spindle checkpoint, causing cell cyclearrest &radic0.18880.8143&radic&radic&radic S000004881PRP24YMR268CverifiedSplicing factor that reanneals U4 and U6 snRNPs duringspliceosome recycling &radic0.186390.81062&radic&radic&radic S000002643PRP42YDR235WverifiedU1 snRNP protein involved in splicing, required for U1 snRNPbiogenesis; contains multipletetriatricopeptide repeats 0.18440.80756&radic&radic&radic&radic S000003096CWC23YGL128CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to E. coliDnaJ and other DnaJ-like proteins and to S.pombe Cwf23p 0.181990.80588&radic&radic&radic S000003510CWC22YGR278WverifiedEssential protein, component of a complex containing Cef1p, hassimilarity to S. pombe Cwf22p; putativespliceosomal component, based on computationalanalysis of large-scale protein-proteininteraction data 0.181750.80544&radic&radic&radic&radic&radic&radic&radic S000001495PRP40YKL012WverifiedU1 snRNP protein involved in splicing, interacts with thebranchpoint-binding protein during theformation of the second commitment complex 0.176560.8&radic&radic S000001208PRP8YHR165CverifiedComponent of the U4/U6-U5 snRNP complex, involved in the secondcatalytic step of splicing &radic0.175820.79957&radic&radic&radic S000004289YHC1YLR298CverifiedComponent of the U1 snRNP complex required for pre-mRNAsplicing; putative ortholog of human U1Cprotein, which is involved in formation of acomplex between U1 snRNP and the pre-mRNA 5'splice site 0.17370.79725&radic&radic S000001578YJU2YKL095WverifiedEssential nuclear protein; putative spliceosomal componentinvolved in mRNA splicing, based oncomputational analysis of large-scaleprotein-protein interaction data 0.16990.79437&radic&radic&radic S000005499CDC33YOL139CverifiedCytoplasmic mRNA cap binding protein; the eIF4E-cap complex isresponsible for mediating cap-dependent mRNAtranslation via interactions with thetranslation initiation factor eIF4G (Tif4631por Tif4632p) 0.167980.79242&radic S000000170AAR2YBL074CverifiedComponent of the U5 snRNP, required for splicing of U3precursors; originally described as a splicingfactor specifically required for splicingpre-mRNA of the MATa1 cistron 0.165620.78829&radic&radic&radic S000002648SNU56YDR240CverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; yeast specific, no metazoancounterpart; interacts with mRNA in commitmentcomplex 0.165770.78829&radic&radic S000002245LUC7YDL087CverifiedEssential protein associated with the U1 snRNP complex;splicing factor involved in recognition of 5'splice site 0.149980.76815&radic&radic S000003245SNU71YGR013WverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; yeast specific, no metazoancounterpart 0.149440.76625&radic&radic&radic S000000356SPP381YBR152WverifiedmRNA splicing factor, component of U4/U6.U5 tri-snRNP;interacts genetically and physically withPrp38p &radic0.148140.76431&radic&radic&radic&radic S000002495SLU7YDR088CverifiedRNA splicing factor, required for ATP-independent portion of2nd catalytic step of spliceosomal RNAsplicing; interacts with Prp18p; contains zincknuckle domain &radic0.14480.7584&radic&radic&radic S000004509PRP39YML046WverifiedU1 snRNP protein involved in splicing, contains multipletetriatricopeptide repeats &radic0.136850.74553&radic&radic S000003238PRP18YGR006WverifiedSplicing factor involved in the positioning of the 3' splicesite during the second catalytic step ofsplicing, recruited to the spliceosome by Slu7p &radic0.134410.74365&radic&radic S000004411YLR419WUncharacterizedPutative helicase with limited sequence similarity to human Rb protein; localized to the cytoplasm and the mitochondria; YLR419W is not an essential gene 0.128650.73377&radic&radic S000003844CSN12YJR084WverifiedSubunit of the Cop9 signalosome, which is required fordeneddylation, or removal of the ubiquitin-likeprotein Rub1p from Cdc53p (cullin); involved inadaptation to pheromone signaling 0.128480.73377&radic&radic&radic S000004735ECM16YMR128WverifiedEssential DEAH-box ATP-dependent RNA helicase specific to theU3 snoRNP, predominantly nucleolar indistribution, required for 18S rRNA synthesis 0.12480.72722&radic&radic&radic S000006261BRR1YPR057WverifiedsnRNP protein component of spliceosomal snRNPs, required forpre-mRNA splicing and snRNP biogenesis; in nullmutant newly-synthesized snRNAs aredestabilized and 3'-end processing is slowed 0.123940.72469&radic&radic S000001561DHR2YKL078WverifiedPredominantly nucleolar DEAH-box RNA helicase, required for 18SrRNA synthesis 0.121760.72315&radic&radic S000001199LIN1YHR156CverifiedNon-essential component of U5 snRNP; nuclear protein;physically interacts with Irr1p of cohesincomplex; may link together proteins involved inchromosome segregation, mRNA splicing and DNAreplication 0.117340.71405&radic&radic&radic S000002691GCN2YDR283CverifiedProtein kinase, phosphorylates the alpha-subunit of translationinitiation factor eIF2 (Sui2p) in response tostarvation; activated by uncharged tRNAs andthe Gcn1p-Gcn20p complex 0.109210.69888&radic&radic&radic S000001083SRB2YHR041CverifiedGeneral transcription factor, subunit of the RNA polymerase IImediator complex; associates with corepolymerase subunits to form the RNA polymeraseII holoenzyme; involved in telomere maintenance 0.104680.6901&radic&radic&radic S000002772CDC40YDR364CverifiedPre-mRNA splicing factor, important for catalytic step II ofpre-mRNA splicing and plays a role in cellcycle progression; required for DNA synthesisduring mitosis and meiosis; has WD repeats &radic0.104030.6898&radic&radic S000004187SIK1YLR197WverifiedEssential evolutionarily-conserved nucleolar protein componentof the box C/D snoRNP complexes that direct2'-O-methylation of pre-rRNA during itsmaturation; overexpression causes spindleorientation defects 0.096030.67402&radic&radic S000000974BRR2YER172CverifiedRNA-dependent ATPase RNA helicase involved in the facilitationand disruption of snRNA interactions, requiredfor disruption of U4/U6 base-pairing in nativesnRNPs to activate the spliceosome forcatalysis &radic0.095140.67288&radic&radic&radic S000005837NOP58YOR310CverifiedProtein involved in pre-rRNA processing, 18S rRNA synthesis,and snoRNA synthesis; component of the smallsubunit processome complex, which is requiredfor processing of pre-18S rRNA 0.092270.6633 S000004106MSL5YLR116WverifiedComponent of the commitment complex, which defines the firststep in the splicing pathway; essential proteinthat interacts with Mud2p and Prp40p, forming abridge between the intron ends; also involvedin nuclear retention of pre-mRNA 0.088340.65148&radic&radic S000004173TOS4YLR183CverifiedTranscription factor that binds to a number of promoterregions, particularly promoters of some genesinvolved in pheromone response and cell cycle;potential Cdc28p substrate; expression isinduced in G1 by bound SBF 0.08620.64503&radic&radic S000005730DED1YOR204WverifiedATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, requiredfor translation initiation of all yeast mRNAs;mutations in human DEAD-box DBY are a frequentcause of male infertility 0.085370.64317 S000001653MRPL38YKL170WverifiedMitochondrial ribosomal protein of the large subunit; appearsas two protein spots (YmL34 and YmL38) ontwo-dimensional SDS gels 0.084170.63927&radic&radic S000004487YML6YML025CverifiedMitochondrial ribosomal protein of the large subunit, hassimilarity to E. coli L4 ribosomal protein andhuman mitoribosomal MRP-L4 protein; essentialfor viability, unlike most other mitoribosomalproteins 0.083510.63775&radic S000004722ASC1YMR116CverifiedWD repeat protein (G-beta like protein) involved in translationregulation; required for repression of Gcn4pactivity in the absence of amino-acidstarvation; core component of the ribosome;ortholog of mammalian RACK1 0.081820.63258 S000001877GNA1YFL017CverifiedEvolutionarily conserved glucosamine-6-phosphateacetyltransferase required for multiple cellcycle events including passage through START,DNA synthesis, and mitosis; involved inUDP-N-acetylglucosamine synthesis, formsGlcNAc6P from AcCoA 0.079960.62911&radic&radic&radic S000002651PRP28YDR243CverifiedRNA helicase in the DEAD-box family, involved in RNAisomerization at the 5' splice site &radic0.07980.6284&radic&radic&radic S000006156RVB2YPL235WverifiedEssential protein involved in transcription regulation;component of chromatin remodeling complexes;required for assembly and function of the INO80complex; member of the RUVB-like protein family 0.074480.609&radic S000000870MOT2YER068WverifiedComponent of the CCR4-NOT complex, which has multiple roles intranscription regulation, mRNA degradation, andpost-transcriptional modifications; with Ubc4p,ubiquitylates subunits of the nascentpolypeptide-associated complex (NAC) 0.073840.60657&radic S000000642IMG1YCR046CverifiedMitochondrial ribosomal protein of the large subunit, requiredfor respiration and for maintenance of themitochondrial genome 0.073530.60628&radic S000002730MRPL35YDR322WverifiedMitochondrial ribosomal protein of the large subunit 0.073070.60388&radic&radic S000003616SCP160YJL080CverifiedEssential RNA-binding G protein effector of mating responsepathway, predominantly associated with nuclearenvelope and ER, interacts in mRNA-dependentmanner with translating ribosomes via multipleKH domains, similar to vertebrate vigilins 0.072760.60273 S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.071490.59629&radic&radic&radic&radic&radic S000004950MRP7YNL005CverifiedMitochondrial ribosomal protein of the large subunit 0.070480.5936&radic S000005168SQS1YNL224CverifiedProtein of unknown function; overexpression antagonizes thesuppression of splicing defects by spp382mutants; green fluorescent protein (GFP)-fusionprotein localizes to both the cytoplasm and thenucleus 0.069260.59102&radic&radic S000000455MRPS5YBR251WverifiedMitochondrial ribosomal protein of the small subunit 0.068670.58933&radic&radic S000004431MRPL4YLR439WverifiedMitochondrial ribosomal protein of the large subunit 0.06780.58506&radic&radic S000002444KRS1YDR037WverifiedLysyl-tRNA synthetase; also identified as a negative regulatorof general control of amino acid biosynthesis 0.066880.58231&radic S000002172NOP1YDL014WverifiedNucleolar protein, component of the small subunit processomecomplex, which is required for processing ofpre-18S rRNA; has similarity to mammalianfibrillarin 0.066740.58103 S000004836UBP8YMR223WverifiedUbiquitin-specific protease that is a component of the SAGA(Spt-Ada-Gcn5-Acetyltransferase) acetylationcomplex; required for SAGA-mediateddeubiquitination of histone H2B 0.066280.58004&radic S000001002YHL010CUncharacterizedPutative protein of unknown function, contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences 0.064730.57507&radic S000003503SLH1YGR271WverifiedPutative RNA helicase related to Ski2p, involved in translationinhibition of non-poly(A) mRNAs; required forrepressing propagation of dsRNA viruses 0.064070.57159 S000004401UTP21YLR409CuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 0.063750.5707&radic&radic&radic S000002633HTA1YDR225WverifiedOne of two nearly identical (see also HTA2) histone H2Asubtypes; core histone required for chromatinassembly and chromosome function; DNAdamage-dependent phosphorylation by Mec1pfacilitates DNA repair; acetylated by Nat4p 0.063660.5707&radic&radic S000002870MRPL28YDR462WverifiedMitochondrial ribosomal protein of the large subunit 0.063380.56872&radic S000004626MRPL3YMR024WverifiedMitochondrial ribosomal protein of the large subunit 0.062760.56763&radic S000005846HSH49YOR319WverifiedU2-snRNP associated splicing factor with similarity to themammalian splicing factor SAP49; proposed tofunction as a U2-snRNP assembly factor alongwith Hsh155p and binding partner Cus1p;contains two RNA recognition motifs (RRM) 0.062520.56702&radic&radic S000003046DBP3YGL078CverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in ribosomal biogenesis 0.062290.56597&radic&radic S000001128NAM8YHR086WverifiedRNA binding protein, component of the U1 snRNP protein; mutantsare defective in meiotic recombination and information of viable spores, involved in theformation of DSBs through meiosis-specificsplicing of MER2 pre-mRNA 0.061630.56367&radic&radic&radic S000001732DBP7YKR024CverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in ribosomal biogenesis 0.061740.56367&radic S000003599MRPL8YJL063CverifiedMitochondrial ribosomal protein of the large subunit 0.060730.56029&radic S000003454PET54YGR222WverifiedProtein required for splicing of the COX1 intron AI5 beta; alsospecifically required, together with Pet122pand Pet494p, for translation of the COX3 mRNA;located in the mitochondrial inner membrane 0.059440.55372&radic S000001107RRP3YHR065CverifiedProtein involved in rRNA processing; required for maturation ofthe 35S primary transcript of pre-rRNA and forcleavage leading to mature 18S rRNA; homologousto eIF-4a, which is a DEAD box RNA-dependentATPase with helicase activity 0.054390.53059&radic&radic&radic S000004652ERB1YMR049CverifiedProtein required for maturation of the 25S and 5.8S ribosomalRNAs; constituent of 66S pre-ribosomalparticles; homologous to mammalian Bop1 0.054060.53031&radic S000003220HFM1YGL251CverifiedMeiosis specific DNA helicase involved in the conversion ofdouble-stranded breaks to later recombinationintermediates and in crossover control;catalyzes the unwinding of Holliday junctions;has ssDNA and dsDNA stimulated ATPase activity 0.050760.51355 S000001894SPB4YFL002CverifiedPutative ATP-dependent RNA helicase, nucleolar protein requiredfor synthesis of 60S ribosomal subunits at alate step in the pathway; sediments with 66Spre-ribosomes in sucrose gradients 0.049460.50576&radic&radic&radic S000001441MPH1YIR002CverifiedMember of the DEAH family of helicases, functions in anerror-free DNA damage bypass pathway thatinvolves homologous recombination, mutationsconfer a mutator phenotype 0.048740.50209&radic&radic S000004903HAS1YMR290CverifiedATP-dependent RNA helicase; localizes to both the nuclearperiphery and nucleolus; highly enriched innuclear pore complex fractions; constituent of66S pre-ribosomal particles 0.047770.49769&radic S000003955YLL032CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene 0.046540.49208 S000000542SRO9YCL037CverifiedCytoplasmic RNA-binding protein that associates withtranslating ribosomes; involved in hemeregulation of Hap1p as a component of the HMCcomplex, also involved in the organization ofactin filaments; contains a La motif 0.045360.48851 S000003394TIF4631YGR162WverifiedTranslation initiation factor eIF4G, subunit of the mRNAcap-binding protein complex (eIF4F) that alsocontains eIF4E (Cdc33p); associates with thepoly(A)-binding protein Pab1p, also interactswith eIF4A (Tif1p); homologous to Tif4632p 0.045220.48806 S000004266DBP9YLR276CverifiedATP-dependent RNA helicase of the DEAD-box family involved inbiogenesis of the 60S ribosomal subunit 0.044480.48165&radic S000005062DCP2YNL118CverifiedCatalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex,which removes the 5' cap structure from mRNAsprior to their degradation; member of the Nudixhydrolase family 0.044320.48165&radic S000006040DBP1YPL119CverifiedPutative ATP-dependent RNA helicase of the DEAD-box proteinfamily; mutants show reduced stability of the40S ribosomal subunit scanning through 5'untranslated regions of mRNAs 0.044650.48165&radic S000003586MTR4YJL050WverifiedDead-box family ATP dependent helicase required for mRNA exportfrom the nucleus; co-factor of the exosomecomplex, required for 3' end formation of 5.8SrRNA 0.044380.48165&radic&radic&radic S000003968RPL8BYLL045CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Ap and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 0.042420.47389&radic S000000262UBP14YBR058CverifiedUbiquitin-specific protease that specifically disassemblesunanchored ubiquitin chains; involved infructose-1,6-bisphosphatase (Fbp1p)degradation; similar to human isopeptidase T 0.042510.47389&radic S000000346MAK5YBR142WverifiedEssential nucleolar protein, putative DEAD-box RNA helicaserequired for maintenance of M1 dsRNA virus;involved in biogenesis of large (60S) ribosomalsubunits 0.041710.47216&radic S000004239YEF3YLR249WverifiedTranslational elongation factor, stimulates the binding ofaminoacyl-tRNA (AA-tRNA) to ribosomes byreleasing EF-1 alpha from the ribosomalcomplex; contains two ABC cassettes; binds andhydrolyses ATP 0.041550.47091 S000000437PBP2YBR233WverifiedRNA binding protein with similarity to mammalian heterogeneousnuclear RNP K protein, involved in theregulation of telomere position effect andtelomere length 0.040910.4695&radic S000002740IRC3YDR332WuncharacterizedHypothetical protein; null mutant displays increased levels ofspontaneous Rad52 foci 0.040590.46884&radic&radic&radic&radic S000002428FAL1YDR021WverifiedNucleolar protein required for maturation of 18S rRNA, memberof the eIF4A subfamily of DEAD-boxATP-dependent RNA helicases 0.040110.46645&radic&radic&radic&radic S000003410PBP1YGR178CverifiedProtein interacting with poly(A)-binding protein Pab1p; likelyinvolved in controlling the extent of mRNApolyadenylation; forms a complex with Mkt1pthat may regulate HO translation; interactswith Lsm12p in a copurification assay 0.040390.46645&radic&radic&radic S000000808NUG1YER006WverifiedGTPase that associates with nuclear 60S pre-ribosomes, requiredfor export of 60S ribosomal subunits from thenucleus 0.040390.46645&radic&radic S000005122RPS3YNL178WverifiedProtein component of the small (40S) ribosomal subunit, hasapurinic/apyrimidinic (AP) endonucleaseactivity; essential for viability; hassimilarity to E. coli S3 and rat S3 ribosomalproteins 0.040180.46645 S000003931DRS1YLL008WverifiedNucleolar DEAD-box protein required for ribosome assembly andfunction, including synthesis of 60S ribosomalsubunits; constituent of 66S pre-ribosomalparticles 0.040530.46645&radic&radic S000003139ROK1YGL171WverifiedATP-dependent RNA helicase of the DEAD box family; required for18S rRNA synthesis 0.039780.4651&radic&radic&radic S000005896RPS12YOR369CverifiedProtein component of the small (40S) ribosomal subunit; hassimilarity to rat ribosomal protein S12 0.039630.46451 S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p 0.03840.45822&radic&radic S000005336NOG2YNR053CverifiedPutative GTPase that associates with pre-60S ribosomal subunitsin the nucleolus and is required for theirnuclear export and maturation 0.037840.45667&radic S000000128HEK2YBL032WverifiedRNA binding protein with similarity to hnRNP-K that localizesto the cytoplasm and to subtelomeric DNA;required for the proper localization of ASH1mRNA; involved in the regulation of telomereposition effect and telomere length 0.036980.45295 S000005056DBP2YNL112WverifiedEssential ATP-dependent RNA helicase of the DEAD-box proteinfamily, involved in nonsense-mediated mRNAdecay and rRNA processing 0.036390.45063&radic&radic S000001212DBP8YHR169WverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in biogenesis of the 40S ribosomalsubunit 0.036290.44894&radic S000005572DBP5YOR046CverifiedCytoplasmic ATP-dependent RNA helicase of the DEAD-box familyinvolved in mRNA export from the nucleus 0.035750.44728 S000006099CBC2YPL178WverifiedSmall subunit of the heterodimeric cap binding complex thatalso contains Sto1p, component of thespliceosomal commitment complex; interacts withNpl3p, possibly to package mRNA for export fromthe nucleus; contains an RNA-binding motif 0.035550.44556&radic&radic&radic S000002242SUB2YDL084WverifiedComponent of the TREX complex required for nuclear mRNA export;member of the DEAD-box RNA helicase superfamilyand is involved in early and late steps ofspliceosome assembly; homolog of the humansplicing factor hUAP56 &radic0.03450.44129&radic&radic S000000564KRR1YCL059CverifiedEssential nucleolar protein required for the synthesis of 18SrRNA and for the assembly of 40S ribosomalsubunit 0.034150.43807&radic S000002334AIR2YDL175CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p; may regulatemethylation of Npl3p, which modulates Npl3pfunction in mRNA processing and export; hassimilarity to Air1p 0.033110.43352&radic&radic S000000441PRP5YBR237WverifiedRNA helicase in the DEAD-box family, necessary forprespliceosome formation, bridges U1 and U2snRNPs and enables stable U2 snRNP associationwith intron RNA &radic0.032680.43235&radic&radic&radic&radic S000001025RPL8AYHL033CverifiedRibosomal protein L4 of the large (60S) ribosomal subunit,nearly identical to Rpl8Bp and has similarityto rat L7a ribosomal protein; mutation resultsin decreased amounts of free 60S subunits 0.032640.4319 S000005199GIS2YNL255CverifiedProtein with seven cysteine-rich CCHC zinc-finger motifs,similar to human CNBP, proposed to be involvedin the RAS/cAMP signaling pathway 0.032590.4319&radic S000002189DBP10YDL031WverifiedPutative ATP-dependent RNA helicase of the DEAD-box proteinfamily, constituent of 66S pre-ribosomalparticles; essential protein involved inribosome biogenesis 0.032260.42879 S000000159KIP1YBL063WverifiedKinesin-related motor protein required for mitotic spindleassembly and chromosome segregation;functionally redundant with Cin8p 0.03110.42451&radic S000004390SKI2YLR398CverifiedPutative RNA helicase, involved in exosome mediated 3' to 5'mRNA degradation and translation inhibition ofnon-poly(A) mRNAs; forms complex with Ski3p andSki8p; required for repressing propagation ofdsRNA viruses 0.030780.42223 S000003032MRH4YGL064CverifiedMitochondrial RNA helicase of the DEAD-box family, plays anessential role in mitochondrial function 0.030690.42223 S000001767TIF1YKR059WverifiedTranslation initiation factor eIF4A, identical to Tif2p;DEA(D/H)-box RNA helicase that couples ATPaseactivity to RNA binding and unwinding; forms adumbbell structure of two compact domainsconnected by a linker; interacts with eIF4G 0.030270.41758 S000005321DBP6YNR038WverifiedEssential protein involved in ribosome biogenesis; putativeATP-dependent RNA helicase of the DEAD-boxprotein family 0.029670.41472&radic S000003570HCA4YJL033WverifiedPutative nucleolar DEAD box RNA helicase; high-copy numbersuppression of a U14 snoRNA processing mutantsuggests an involvement in 18S rRNA synthesis 0.028790.40929 S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus 0.028870.40929&radic&radic&radic&radic&radic S000003674TIF2YJL138CverifiedTranslation initiation factor eIF4A, identical to Tif1p;DEA(D/H)-box RNA helicase that couples ATPaseactivity to RNA binding and unwinding; forms adumbbell structure of two compact domainsconnected by a linker; interacts with eIF4G 0.02890.40929 S000003131RAD54YGL163CverifiedDNA-dependent ATPase, stimulates strand exchange by modifyingthe topology of double-stranded DNA; involvedin the recombinational repair of double-strandbreaks in DNA during vegetative growth andmeiosis; member of the SWI/SNF family 0.028620.40479&radic S000002243NDE2YDL085WverifiedMitochondrial external NADH dehydrogenase, catalyzes theoxidation of cytosolic NADH; Nde1p and Nde2pare involved in providing the cytosolic NADH tothe mitochondrial respiratory chain 0.027570.40169 S000004685NAM7YMR080CverifiedATP-dependent RNA helicase of the SFI superfamily, required fornonsense mediated mRNA decay and for efficienttranslation termination at nonsense codons;involved in telomere maintenance 0.027080.39766&radic S000000449ISW1YBR245CverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPase thatforms a complex with Ioc2p and Ioc4p toregulate transcription elongation, and acomplex with Ioc3p to repress transcriptioninitiation 0.026870.39766&radic&radic&radic S000001557MUD2YKL074CverifiedProtein involved in early pre-mRNA splicing; component of thepre-mRNA-U1 snRNP complex, the commitmentcomplex; interacts with Msl5p/BBP splicingfactor and Sub2p; similar to metazoan splicingfactor U2AF65 &radic0.026480.39476&radic&radic&radic&radic&radic S000004122YLR132CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to mitochondria and nucleus; YLR132C is an essential gene 0.026330.39355&radic S000002814PDR15YDR406WverifiedATP binding cassette (ABC) transporter of the plasma membrane;general stress response factor implicated incellular detoxification; target of Pdr1p, Pdr3pand Pdr8p transcription regulators; promotercontains a PDR responsive element 0.026230.39108&radic&radic S000006333SCD6YPR129WverifiedProtein containing an Lsm domain, may bind RNA and have a rolein RNA processing; overproduction suppresses anull mutation in CHC1, which encodes the heavychain of clathrin 0.025610.38764 S000003017TIF4632YGL049CverifiedTranslation initiation factor eIF4G, subunit of the mRNAcap-binding protein complex (eIF4F) that alsocontains eIF4E (Cdc33p); associates with thepoly(A)-binding protein Pab1p, also interactswith eIF4A (Tif1p); homologous to Tif4631p 0.025350.38669 S000004022RAD5YLR032WverifiedSingle-stranded DNA-dependent ATPase, involved inpostreplication repair; contains RING fingerdomain 0.025220.38461&radic&radic&radic&radic S000002777XRS2YDR369CverifiedProtein required for DNA repair; component of the Mre11complex, which is involved in double strandbreaks, meiotic recombination, telomeremaintenance, and checkpoint signaling 0.024770.37639&radic&radic&radic&radic&radic&radic S000005230CUS2YNL286WverifiedProtein that binds to U2 snRNA and Prp11p, may be involved inU2 snRNA folding; contains two RNA recognitionmotifs (RRMs) 0.024280.37639&radic&radic S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif 0.024080.37521&radic&radic S000004842RRP5YMR229CverifiedProtein required for the synthesis of both 18S and 5.8S rRNA;C-terminal region is crucial for the formationof 18S rRNA and N-terminal region is requiredfor the 5.8S rRNA; component of small ribosomalsubunit (SSU) processosome 0.023720.37365&radic S000002372NOP6YDL213CuncharacterizedProtein with similarity to hydrophilins, which are involved inthe adaptive response to hyperosmoticconditions; computational analysis oflarge-scale protein-protein interaction datasuggests a possible role in rRNA processing 0.023690.37027&radic&radic S000005337ESF2YNR054CverifiedEssential nucleolar protein involved in pre-18S rRNAprocessing; component of the small subunit(SSU) processome; has sequence similarity tomABT1, a mouse transcription activator 0.023310.36967&radic S000002744YDR336WUncharacterizedPutative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene 0.02280.36661&radic&radic&radic&radic&radic S000002602MSS116YDR194CverifiedDEAD-box protein required for efficient splicing ofmitochondrial Group I and II introns; non-polarRNA helicase that also facilities strandannealing &radic0.022770.36647&radic S000001380RHO3YIL118WverifiedNon-essential small GTPase of the Rho/Rac subfamily of Ras-likeproteins involved in the establishment of cellpolarity; GTPase activity positively regulatedby the GTPase activating protein (GAP) Rgd1p 0.022050.35988&radic&radic S000002699HRQ1YDR291WuncharacterizedPutative DNA helicase 0.021340.35245&radic S000002742SWR1YDR334WverifiedSwi2/Snf2-related ATPase that is the structural component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.020870.35061&radic&radic&radic&radic&radic S000005950SUV3YPL029WverifiedATP-dependent RNA helicase, component of the mitochondrialdegradosome along with the RNase Dss1p; thedegradosome associates with the ribosome andmediates turnover of aberrant or unprocessedRNAs 0.020730.35009&radic S000004267YSH1YLR277CverifiedPutative endonuclease, subunit of the mRNA cleavage andpolyadenylation specificity complex requiredfor 3' processing of mRNAs 0.019870.34096&radic&radic&radic S000001505CDC16YKL022CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; required forsporulation 0.019870.34096&radic&radic&radic&radic S000000277RDH54YBR073WverifiedDNA-dependent ATPase, stimulates strand exchange by modifyingthe topology of double-stranded DNA; involvedin the recombinational repair of double-strandbreaks in DNA during mitosis and meiosis;proposed to be involved in crossoverinterference 0.019580.33959&radic&radic S000001261YHR218WUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.019210.33673&radic&radic S000000317YBR113WDubiousHypothetical protein 0.019230.33673&radic S000001828YFL066CUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.019120.3342 S000003388PTI1YGR156WverifiedPta1p Interacting protein 0.018430.32984&radic&radic&radic S000003796RAD26YJR035WverifiedProtein involved in transcription-coupled repair nucleotideexcision repair of UV-induced DNA lesions;homolog of human CSB protein 0.018460.32984&radic&radic S000004665RNA14YMR061WverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; bridges interaction between Rna15p andHrp1p in the CF I complex 0.018290.32607&radic&radic&radic S000003740RCY1YJL204CverifiedF-box protein involved in recycling plasma membrane proteinsinternalized by endocytosis; localized to sitesof polarized growth 0.017750.32346&radic&radic&radic S000000992YRF1-2YER190WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.017490.32076&radic&radic S000000318RAD16YBR114WverifiedProtein that recognizes and binds damaged DNA in anATP-dependent manner (with Rad7p) duringnucleotide excision repair; subunit ofNucleotide Excision Repair Factor 4 (NEF4);member of the SWI/SNF family 0.017210.31766&radic S000002857UTP6YDR449CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 0.017270.31766 S000005083RLR1YNL139CverifiedSubunit of the THO complex, which is required for efficienttranscription elongation and involved intranscriptional elongation-associatedrecombination; required for LacZ RNA expressionfrom certain plasmids 0.017210.31766&radic&radic S000005493HRT1YOL133WverifiedRING finger containing subunit of Skp1-Cullin-F-box ubiquitinprotein ligases (SCF); required for Gic2p,Far1p, Sic1p and Cln2p degradation; may tetherCdc34p (a ubiquitin conjugating enzyme or E2)and Cdc53p (a cullin) subunits of SCF 0.016740.31427 S000000017FUN30YAL019WverifiedProtein whose overexpression affects chromosome stability,potential Cdc28p substrate; homolog of Snf2p;the authentic, non-tagged protein is detectedin highly purified mitochondria inhigh-throughput studies 0.016620.31383&radic&radic S000001405SSL2YIL143CverifiedComponent of the holoenzyme form of RNA polymerasetranscription factor TFIIH, has DNA-dependentATPase/helicase activity and is required, withRad3p, for unwinding promoter DNA; involved inDNA repair; homolog of human ERCC3 0.016180.30861&radic&radic S000004237IRC20YLR247CuncharacterizedPutative helicase; localized to mitochondria and the nucleus;YLR247C is not an essential gene; null mutantdisplays increased levels of spontaneous Rad52foci 0.016060.30763&radic S000002587YDR179W-AUncharacterizedHypothetical protein 0.015990.3056&radic&radic&radic S000003118INO80YGL150CverifiedATPase that forms a large complex, containing actin and severalactin-related proteins, that has chromatinremodeling activity and 3' to 5' DNA helicaseactivity in vitro; shows similarity to theSnf2p family of ATPases 0.015940.3056&radic S000004802SGS1YMR190CverifiedNucleolar DNA helicase of the RecQ family involved inmaintenance of genome integrity, regulateschromosome synapsis and meiotic crossing over;has similarity to human BLM and WRN helicasesimplicated in Bloom and Werner syndromes 0.01530.3002&radic S000005659EFT1YOR133WverifiedElongation factor 2 (EF-2), also encoded by EFT2; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 0.015460.3002 S000002545HPR1YDR138WverifiedSubunit of THO/TREX complexes that couple transcriptionelongation with mitotic recombination and withmRNA metabolism and export, subunit of an RNAPol II complex; regulates lifespan; involved intelomere maintenance; similar to Top1p 0.015420.3002&radic&radic&radic S000003528YRF1-3YGR296WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.015170.29715 S000001388STH1YIL126WverifiedATPase component of the ATP-dependent RSC chromatin remodelingcomplex required for kinetochore function inchromosome segregation; required for expressionof early meiotic genes; essentialhelicase-related protein homologous to Snf2p 0.015090.29715&radic&radic S000001697YRA2YKL214CverifiedMember of the REF (RNA and export factor binding proteins)family; when overexpressed, can substitute forthe function of Yra1p in export of poly(A)+mRNA from the nucleus 0.014910.29625&radic&radic S000004021YLR031WUncharacterizedHypothetical protein 0.014820.29468&radic&radic&radic S000000883SER3YER081Wverified3-phosphoglycerate dehydrogenase, catalyzes the first step inserine and glycine biosynthesis; isozyme ofSer33p 0.01480.29468&radic&radic&radic S000007526YOR396WUncharacterizedHypothetical protein 0.014790.29441 S000006003MOT1YPL082CverifiedEssential abundant protein involved in regulation oftranscription, removes Spt15p (TBP) from DNAvia its C-terminal ATPase activity, forms acomplex with TBP that binds TATA DNA with highaffinity but with altered specificity 0.014790.29441&radic&radic S000005816SNF2YOR290CverifiedCatalytic subunit of the SWI/SNF chromatin remodeling complexinvolved in transcriptional regulation;contains DNA-stimulated ATPase activity;functions interdependently in transcriptionalactivation with Snf5p and Snf6p 0.014650.29261&radic&radic S000002998RPL30YGL030WverifiedProtein component of the large (60S) ribosomal subunit, hassimilarity to rat L30 ribosomal protein;involved in pre-rRNA processing in thenucleolus; autoregulates splicing of itstranscript 0.014610.29261 S000002586CSN9YDR179CverifiedSubunit of the Cop9 signalosome, which is required fordeneddylation, or removal of the ubiquitin-likeprotein Rub1p from Cdc53p (cullin); involved inadaptation to pheromone signaling 0.014620.29261&radic&radic&radic S000000939YER137CUncharacterizedHypothetical protein 0.014560.28925 S000001439YIL177CUncharacterizedPutative protein of unknown function; similarity to DNA helicases that are encoded within subtelomeric Y' elements and induced in telomerase deficient survivors 0.014430.28925&radic S000003989YLL066CUncharacterizedPutative protein of unknown function with similarity to helicases; YLL066C is not an essential gene 0.014410.28925 S000001934IRC5YFR038WuncharacterizedPutative ATPase containing the DEAD/H helicase-related sequencemotif; null mutant displays increased levels ofspontaneous Rad52 foci 0.014220.28728&radic S000004602YML133CUncharacterizedPutative protein of unknown function with similarity to helicases; localized to mitochondria; YML133C contains an intron 0.014280.28728 S000003760YJL225CUncharacterizedPutative protein of unknown function; similarity to DNA helicases that are also encoded within subtelomeric Y' elements and induced in telomerase deficient survivors 0.014250.28728&radic S000005651CAT5YOR125CverifiedMitochondrial inner membrane protein directly involved inubiquinone biosynthesis, essential for severalother metabolic pathways including respirationand gluconeogenic gene activation 0.014230.28728&radic&radic S000002776YPR1YDR368Wverified2-methylbutyraldehyde reductase, may be involved in isoleucinecatabolism 0.014020.28601&radic S000002830SIP1YDR422CverifiedAlternate beta-subunit of the Snf1p kinase complex, may confersubstrate specificity; vacuolar proteincontaining KIS (Kinase-Interacting Sequence)and ASC (Association with Snf1 kinase Complex)domains involved in protein interactions 0.013950.28429 S000000966CHD1YER164WverifiedNucleosome remodeling factor that functions in regulation oftranscription elongation; contains a chromodomain, a helicase domain and a DNA-bindingdomain; component of both the SAGA and SILKcomplexes 0.013850.28407&radic S000004840TAF7YMR227CverifiedTFIID subunit (67 kDa), involved in RNA polymerase IItranscription initiation 0.013690.28107&radic&radic&radic&radic&radic S000002466UBC5YDR059CverifiedUbiquitin-conjugating enzyme that mediates selectivedegradation of short-lived and abnormalproteins, central component of the cellularstress response; expression is heat inducible 0.013790.28107&radic S000002491TVP23YDR084CuncharacterizedIntegral membrane protein localized to late Golgi vesiclesalong with the v-SNARE Tlg2p; green fluorescentprotein (GFP)-fusion protein localizes to thecytoplasm in a punctate pattern 0.013340.27697&radic&radic S000000217YBR013CUncharacterizedHypothetical protein 0.013570.27697&radic&radic S000003990YLL067CUncharacterizedPutative protein of unknown function with similarity to helicases 0.013440.27697 S000002837TIF35YDR429CverifiedSubunit of the core complex of translation initiation factor3(eIF3), which is essential for translation 0.013530.27697&radic S000004846YMR233WUncharacterizedProtein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; YMR233W is not an essential gene 0.013530.27697&radic&radic&radic&radic&radic&radic S000004059MEF1YLR069CverifiedMitochondrial elongation factor involved in translationalelongation 0.013240.2764&radic S000003201TAN1YGL232WverifiedPutative tRNA acetyltransferase, RNA-binding protein requiredfor the formation of the modified nucleosideN(4)-acetylcytidine in serine and leucine tRNAsbut not required for the same modification in18S rRNA 0.013030.27397&radic&radic&radic&radic&radic S000000004SSA1YAL005CverifiedATPase involved in protein folding and nuclear localizationsignal (NLS)-directed nuclear transport; memberof heat shock protein 70 (HSP70) family; formsa chaperone complex with Ydj1p; localized tothe nucleus, cytoplasm, and cell wall 0.012940.27334&radic&radic&radic S000002259DUN1YDL101CverifiedCell-cycle checkpoint serine-threonine kinase required for DNAdamage-induced transcription of certain targetgenes, phosphorylation of Rad55p and Sml1p, andtransient G2/M arrest after DNA damage; alsoregulates postreplicative DNA repair 0.012690.26994&radic&radic&radic&radic S000005831ISW2YOR304WverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPasecomponent that, with Itc1p, forms a complexrequired for repression of a-specific genes,INO1, and early meiotic genes during mitoticgrowth 0.012730.26994&radic&radic&radic S000000517GBP2YCL011CverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toHrb1p and Npl3p; also binds single-strandedtelomeric repeat sequence in vitro 0.01260.26917&radic S000006204YRF1-7YPL283CverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.012390.26492 S000002789YRA1YDR381WverifiedNuclear protein that binds to RNA and to Mex67p, required forexport of poly(A)+ mRNA from the nucleus;member of the REF (RNA and export factorbinding proteins) family; another familymember, Yra2p, can substitute for Yra1pfunction 0.012470.26492&radic&radic S000002764SPC110YDR356WverifiedInner plaque spindle pole body (SPB) component, ortholog ofhuman kendrin; involved in connecting nuclearmicrotubules to SPB; interacts withTub4p-complex and calmodulin; phosphorylated byMps1p in cell cycle-dependent manner 0.012450.26492&radic&radic S000004949HRB1YNL004WverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toGbp2p and Npl3p 0.012480.26492&radic S000003767SUI2YJR007WverifiedAlpha subunit of the translation initiation factor eIF2,involved in the identification of the startcodon; phosphorylation of Ser51 is required forregulation of translation by inhibiting theexchange of GDP for GTP 0.012290.26492&radic&radic&radic S000005283YRF1-6YNL339CverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.012110.26147 S000002349UFD2YDL190CverifiedUbiquitin chain assembly factor (E4) that cooperates with aubiquitin-activating enzyme (E1), aubiquitin-conjugating enzyme (E2), and aubiquitin protein ligase (E3) to conjugateubiquitin to substrates; also functions as an 0.012150.26147&radic&radic&radic S000002151YBL111CUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.011920.26066 S000004459YRF1-5YLR467WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.011910.26066 S000001500HCS1YKL017CverifiedHexameric DNA polymerase alpha-associated DNA helicase Ainvolved in lagging strand DNA synthesis;contains single-stranded DNA stimulated ATPaseand dATPase activities; replication protein Astimulates helicase and ATPase activities 0.011880.26042&radic&radic S000006408YPR204WUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.011780.2592 S000003200YGL231CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum 0.011840.2592&radic&radic&radic S000000203YBL107CUncharacterizedHypothetical protein 0.011740.25795&radic&radic S000005119NOP13YNL175CverifiedProtein of unknown function, localizes to the nucleolus andnucleoplasm; contains an RNA recognition motif(RRM) and has similarity to Nop12p, which isrequired for processing of pre-18S rRNA 0.01160.25625&radic&radic S000002493SSS1YDR086CverifiedSubunit of the Sec61p translocation complex(Sec61p-Sss1p-Sbh1p) that forms a channel forpassage of secretory proteins through theendoplasmic reticulum membrane, and of theSsh1p complex (Ssh1p-Sbh2p-Sss1p); interactswith Ost4p and Wbp1p 0.011230.25117&radic&radic S000006409YEL077CUncharacterizedHelicase-like protein encoded within the telomeric Y' element 0.011240.25117 S000002200SIR2YDL042CverifiedConserved NAD+ dependent histone deacetylase of the Sirtuinfamily involved in regulation of lifespan;plays roles in silencing at HML, HMR,telomeres, and the rDNA locus; negativelyregulates initiation of DNA replication 0.011110.24916&radic S000004458YRF1-4YLR466WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.011050.24916 S000005202DSL1YNL258CverifiedEndoplasmic reticulum (ER)-localized peripheral membraneprotein required for Golgi-to-ER retrogradetraffic; component of the ER target site thatinteracts with coatomer, the major component ofthe COPI vesicle protein coat 0.010790.24542&radic&radic&radic S000003423HIP1YGR191WverifiedHigh-affinity histidine permease, also involved in thetransport of manganese ions 0.01080.24542&radic S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 0.010610.24336&radic&radic&radic S000006311YTH1YPR107CverifiedEssential RNA-binding component of cleavage and polyadenylationfactor, contains five zinc fingers; requiredfor pre-mRNA 3'-end processing andpolyadenylation 0.010610.24336&radic&radic&radic S000002953YRF1-1YDR545WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 0.010570.24139 S000006044RNY1YPL123CverifiedRNAse; member of the T(2) family of endoribonucleases 0.010590.24139&radic&radic&radic&radic S000005107RIA1YNL163CverifiedCytoplasmic GTPase involved in biogenesis of the 60S ribosome;has similarity to translation elongation factor2 (Eft1p and Eft2p) 0.010510.24139 S000003162HOS2YGL194CverifiedHistone deacetylase required for gene activation via specificdeacetylation of lysines in H3 and H4 histonetails; subunit of the Set3 complex, ameiotic-specific repressor of sporulationspecific genes that contains deacetylaseactivity 0.010350.23964&radic&radic&radic&radic&radic S000005171JJJ1YNL227CverifiedProtein that contains a 70 amino acid J-domain, may function asa co-chaperone to recruit Hsp70-like activityto specific sites; mutation causes defects influid-phase endocytosis 0.010350.23964&radic&radic S000001444IST3YIR005WverifiedComponent of the U2 snRNP, required for the first catalyticstep of splicing and for spliceosomal assembly;interacts with Rds3p and is required forMer1p-activated splicing &radic0.010250.23807&radic&radic S000001953YFR057WUncharacterizedHypothetical protein 0.010180.23692&radic S000006373JIP5YPR169WuncharacterizedNucleolar protein of unknown function, exhibits a physicalinteraction with Bre1p 0.010030.23472&radic&radic&radic&radic&radic&radic S000002793EFT2YDR385WverifiedElongation factor 2 (EF-2), also encoded by EFT1; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 0.010110.23472 S000000294YBR090CUncharacterizedHypothetical protein 0.010.23407&radic&radic S000000378YBR174CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective 0.009820.23051&radic&radic S000000216YBR012CDubiousHypothetical protein 0.009830.23051&radic&radic S000003360UTP8YGR128CverifiedNucleolar protein required for export of tRNAs from thenucleus; also copurifies with the small subunit(SSU) processome containing the U3 snoRNA thatis involved in processing of pre-18S rRNA 0.009660.22804&radic&radic S000002490RRP8YDR083WverifiedNucleolar protein involved in rRNA processing, pre-rRNAcleavage at site A2; also involved in telomeremaintenance; mutation is synthetically lethalwith a gar1 mutation 0.009730.22804&radic&radic&radic&radic S000002831CAD1YDR423CverifiedAP-1-like bZIP transcriptional activator involved in multiplestress responses, iron metabolism, andpleiotropic drug resistance; controls a set ofgenes involved in stabilizing proteins, bindsconsensus sequence TTACTAA; 5' UTR containsuORFs 0.009760.22804&radic&radic&radic&radic S000000967PAB1YER165WverifiedPoly(A) binding protein, part of the 3'-end RNA-processingcomplex, mediates interactions between the 5'cap structure and the 3' mRNA poly(A) tail,involved in control of poly(A) tail length,interacts with translation factor eIF-4G 0.009770.22804&radic S000005519YOL159CUncharacterizedHypothetical protein 0.009570.22678&radic&radic&radic S000004693YMR087WVerifiedPutative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain 0.009560.22647&radic&radic S000003670LCB3YJL134WverifiedLong-chain base-1-phosphate phosphatase, regulates ceramide andlong-chain base phosphates levels, involved inincorporation of exogenous long chain bases insphingolipids 0.00940.22372 S000006390PZF1YPR186CverifiedTranscription factor IIIA (TFIIIA), essential protein with nineC2H2 Zn-fingers, binds the 5S rRNA gene throughthe zinc finger domain and directs assembly ofa multiprotein initiation complex for RNApolymerase III; also binds DNA 0.00950.22372&radic&radic&radic&radic S000001924CDC14YFR028CverifiedProtein phosphatase required for mitotic exit; located in thenucleolus until liberated by the FEAR andMitotic Exit Network in anaphase, enabling itto act on key substrates to effect a decreasein CDK/B-cyclin activity and mitotic exit 0.009380.22372&radic&radic&radic&radic S000001526PHD1YKL043WverifiedTranscriptional activator that enhances pseudohyphal growth;regulates expression of FLO11, an adhesinrequired for pseudohyphal filament formation;similar to StuA, an A. nidulans developmentalregulator; potential Cdc28p substrate 0.009330.22287&radic&radic&radic&radic S000005150RTT106YNL206CverifiedProtein with a role in regulation of Ty1 transposition 0.009330.22287&radic&radic&radic S000002209LHP1YDL051WverifiedRNA binding protein required for maturation of tRNA and snRNAprecursors; acts as a molecular chaperone forRNAs transcribed by polymerase III; homologousto human La (SS-B) autoantigen 0.009230.22059&radic S000000115APN2YBL019WverifiedClass II abasic (AP) endonuclease involved in repair of DNAdamage; homolog of human HAP1 and E. coliexoIII 0.009160.2201&radic&radic&radic S000003712SWI3YJL176CverifiedSubunit of the SWI/SNF chromatin remodeling complex, whichregulates transcription by remodelingchromosomes; required for transcription of manygenes, including ADH1, ADH2, GAL1, HO, INO1 andSUC2 0.009090.21791&radic&radic S000002812RPB7YDR404CverifiedRNA polymerase II subunit B16; forms two subunit dissociablecomplex with Rpb4p 0.009030.21633&radic S000002284CDC48YDL126CverifiedATPase in ER, nuclear membrane and cytosol with homology tomammalian p97; in a complex with Npl4p andUfd1p participates in retrotranslocation ofubiquitinated proteins from the ER into thecytosol for degradation by the proteasome 0.008980.21633&radic&radic S000002537FIN1YDR130CverifiedSpindle pole body-related intermediate filament protein, formscell cycle-specific filaments between spindlepole bodies in mother and daughter cells, ableto self-assemble, expression induced duringS/G2, localization cell-cycle dependent 0.008870.21434&radic&radic&radic S000002801SHE9YDR393WverifiedMitochondrial inner membrane protein required for normalmitochondrial morphology, may be involved infission of the inner membrane; forms ahomo-oligomeric complex 0.008850.21371&radic&radic&radic S000001026SBP1YHL034CverifiedNucleolar single-strand nucleic acid binding protein;associates with small nuclear RNAs 0.008840.21371&radic S000005450MSH2YOL090WverifiedProtein that forms heterodimers with Msh3p and Msh6p that bindto DNA mismatches to initiate the mismatchrepair process; contains a Walker ATP-bindingmotif required for repair activity; Msh2p-Msh6pbinds to and hydrolyzes ATP 0.00880.2129&radic&radic&radic&radic&radic&radic S000005381DIS3YOL021CverifiedNucleolar exosome component, involved in rRNA processing andRNA degradation, binds Gsp1p/Ran and enhancesthe GEF activity of Srm1p, implicated inmitotic control, homologous to the E. coliRNase R of the RNase II family 0.008740.21024&radic&radic S000003189NIF3YGL221CverifiedProtein of unknown function, similar to Listeria monocytogenesmajor sigma factor (rpoD gene product); theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 0.008770.21024&radic S000002475DOS2YDR068WverifiedProtein of unknown function, green fluorescent protein(GFP)-fusion protein localizes to the cytoplasm 0.008640.20976&radic&radic S000004165CBF5YLR175WverifiedPseudouridine synthase catalytic subunit of box H/ACA smallnucleolar ribonucleoprotein particles(snoRNPs), acts on both large and small rRNAsand on snRNA U2 0.008610.20969&radic&radic&radic&radic S000002468YDR061WUncharacterizedMitochondrial protein, member of the ATP-binding cassette (ABC) transporter family; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance 0.008590.20873&radic&radic&radic S000005506PSF3YOL146WverifiedSubunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), whichis localized to DNA replication origins andimplicated in assembly of the DNA replicationmachinery 0.008560.20873&radic&radic&radic&radic S000005160RAP1YNL216WverifiedDNA-binding protein involved in either activation or repressionof transcription, depending on binding sitecontext; also binds telomere sequences andplays a role in telomeric position effect(silencing) and telomere structure 0.008540.20827&radic&radic S000002725HIM1YDR317WverifiedProtein of unknown function involved in DNA repair 0.008540.20827&radic&radic S000003851JSN1YJR091CverifiedMember of the Puf family of RNA-binding proteins, interactswith mRNAs encoding membrane-associatedproteins; overexpression suppresses a tub2-150mutation and causes increased sensitivity tobenomyl in wild-type cells 0.008510.20686 S000001112TRM5YHR070WverifiedtRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacentto the anticodon that has a role in preventionof frameshifting; highly conserved acrossArchaea, Bacteria, and Eukarya 0.008460.20686&radic&radic S000004156SEC10YLR166CverifiedEssential 100kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 0.008410.20493&radic S000001897LOC1YFR001WverifiedNuclear protein involved in asymmetric localization of ASH1mRNA; binds double-stranded RNA in vitro;constituent of 66S pre-ribosomal particles 0.008380.20493&radic&radic&radic&radic S000001446YIR007WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene 0.008320.20473&radic S000000539LSB5YCL034WverifiedProtein of unknown function; binds Las17p, which is a homologof human Wiskott-Aldrich Syndrome proteininvolved in actin patch assembly and actinpolymerization 0.00830.20449&radic&radic&radic S000002828HKR1YDR420WverifiedSerine/threonine rich cell surface protein that contains an EFhand motif; involved in the regulation of cellwall beta-1,3 glucan synthesis and bud siteselection; overexpression confers resistance toHansenula mrakii killer toxin, HM-1 0.00830.20449 S000000678AHC2YCR082WuncharacterizedProtein of unknown function, putative transcriptionalregulator; proposed to be a Ada Histoneacetyltransferase complex component; GFP taggedprotein is localized to the cytoplasm andnucleus 0.00830.20449&radic&radic&radic&radic&radic S000002690YDR282CUncharacterizedHypothetical protein 0.008260.20284&radic&radic S000004039YLR049CUncharacterizedHypothetical protein 0.008280.20284&radic S000000155YBL059WUncharacterizedHypothetical protein 0.00820.20275&radic&radic S000000063NUP60YAR002WverifiedSubunit of the nuclear pore complex (NPC), functions to anchorNup2p to the NPC in a process controlled by thenucleoplasmic concentration of Gsp1p-GTP;potential Cdc28p substrate; involved intelomere maintenance 0.008190.20226&radic&radic&radic S000000575YCL073CUncharacterizedProtein of unconfirmed function; displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins; coding sequence 98% identical to that of YKR106W 0.008090.20061&radic&radic&radic S000004072SRL2YLR082CverifiedProtein of unknown function; overexpression suppresses thelethality caused by a rad53 null mutation 0.008140.20061&radic&radic&radic S000002510STE5YDR103WverifiedPheromone-responsive scaffold protein; shuttles from thenucleus to the plasma membrane and assembleskinases Ste11p, Ste7p, and Fus3p into aspecific signaling complex; active oligomericform interacts with Ste4p-Ste18p complex 0.008040.19915&radic&radic S000005783CDC31YOR257WverifiedComponent of the spindle pole body (SPB) half-bridge, requiredfor SPB duplication in mitosis and meiosis II;homolog of mammalian centrin; interacts withKar1p 0.0080.19915&radic&radic&radic&radic S000003199YGL230CUncharacterizedHypothetical protein 0.007910.198&radic&radic&radic&radic&radic S000001220YHR177WUncharacterized 0.007870.1972&radic&radic&radic&radic S000005440REX4YOL080CverifiedPutative RNA exonuclease possibly involved in pre-rRNAprocessing and ribosome assembly 0.007780.19466&radic&radic&radic S000001200REC104YHR157WverifiedProtein involved in early stages of meiotic recombination;required for meiotic crossing over; forms acomplex with Rec102p and Spo11p necessaryduring the initiation of recombination 0.00770.19428&radic S000001057MIP6YHR015WverifiedPutative RNA-binding protein, interacts with Mex67p, which is acomponent of the nuclear pore involved innuclear mRNA export 0.007650.19335 S000000147PIN4YBL051CverifiedProtein involved in G2/M phase progression and response to DNAdamage, interacts with Rad53p; contains an RNArecognition motif, a nuclear localizationsignal, and several SQ/TQ cluster domains;hyperphosphorylated in response to DNA damage 0.007580.19253&radic&radic S000001764TRM2YKR056WverifiedtRNA methyltransferase, 5-methylates the uridine residue atposition 54 of tRNAs and may also have a rolein tRNA stabilization or maturation; previouslythought to be an endo-exonuclease 0.007560.19151&radic S000001146GRE3YHR104WverifiedAldose reductase involved in methylglyoxal, d-xylose andarabinose metabolism; stress induced (osmotic,ionic, oxidative, heat shock, starvation andheavy metals); regulated by the HOG pathway 0.007530.19151&radic&radic&radic S000004093CDC45YLR103CverifiedDNA replication initiation factor; recruited to MCM pre-RCcomplexes at replication origins; promotesrelease of MCM from Mcm10p, recruits elongationmachinery; mutants in human homolog may causevelocardiofacial and DiGeorge syndromes 0.007530.19111&radic&radic S000002504MSH6YDR097CverifiedProtein required for mismatch repair in mitosis and meiosis,forms a complex with Msh2p to repair bothsingle-base & insertion-deletion mispairs;potentially phosphorylated by Cdc28p 0.007450.19018&radic&radic&radic&radic S000001069RPN1YHR027CverifiedNon-ATPase base subunit of the 19S regulatory particle of the26S proteasome; may participate in therecognition of several ligands of theproteasome; contains a leucine-rich repeat(LRR) domain, a site for protein?proteininteractions 0.007420.18924&radic&radic&radic&radic&radic S000001554YKL071WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.007260.18582 S000001042YHL050CUncharacterizedPutative protein of unknown function, potential Cdc28p substrate 0.007290.18582 S000004852RNT1YMR239CverifiedRNAase III; cleaves a stem-loop structure at the 3' end of U2snRNA to ensure formation of the correct U2 3'end 0.007260.18582&radic&radic&radic&radic&radic&radic S000004703MTG1YMR097CverifiedPeripheral GTPase of the mitochondrial inner membrane,essential for respiratory competence, likelyfunctions in assembly of the large ribosomalsubunit, has homologs in plants and animals 0.007250.18582&radic&radic&radic S000003212TAD1YGL243WverifiedtRNA-specific adenosine deaminase, deaminates adenosine-37 toinosine in tRNA-Ala 0.00720.18524&radic&radic&radic&radic&radic S000002152YBL112CUncharacterizedPutative protein of unknown function; YBL112C is contained within TEL02L 0.007110.18319 S000005401NOP12YOL041CverifiedNucleolar protein, required for pre-25S rRNA processing;contains an RNA recognition motif (RRM) and hassimilarity to Nop13p, Nsr1p, and putativeorthologs in Drosophila and S. pombe 0.007050.18253&radic S000002778YDR370CUncharacterizedHypothetical protein 0.007030.18177&radic&radic S000000452HIS7YBR248CverifiedImidazole glycerol phosphate synthase (glutamineamidotransferase:cyclase), catalyzes the fifthand sixth steps of histidine biosynthesis andalso produces 5-aminoimidazole-4-carboxamideribotide (AICAR), a purine precursor 0.006960.18016&radic&radic S000005842SFG1YOR315WverifiedNuclear protein, putative transcription factor required forgrowth of superficial pseudohyphae (which donot invade the agar substrate) but not forinvasive pseudohyphal growth; may act togetherwith Phd1p; potential Cdc28p substrate 0.006920.1799&radic S000006111NAB3YPL190CverifiedSingle stranded DNA binding protein; acidic ribonucleoprotein;required for termination of non-poly(A)transcripts and efficient splicing; interactswith Nrd1p 0.006710.17578&radic&radic S000006380BET2YPR176CverifiedBeta subunit of Type II geranylgeranyltransferase required forvesicular transport between the endoplasmicreticulum and the Golgi; provides a membraneattachment moiety to Rab-like proteins Ypt1pand Sec4p 0.006660.17302&radic&radic&radic S000005556DFG16YOR030WverifiedProbable multiple transmembrane protein, involved in diploidinvasive and pseudohyphal growth upon nitrogenstarvation; required for accumulation ofprocessed Rim101p 0.006610.17302 S000003482YGR250CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0.006540.17149&radic&radic S000001259IMD2YHR216WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, expression is induced bymycophenolic acid resulting in resistance tothe drug, expression is repressed by nutrientlimitation 0.006480.17044&radic&radic S000004961PUB1YNL016WverifiedPoly(A)+ RNA-binding protein, abundant mRNP-component proteinthat binds mRNA and is required for stabilityof a number of mRNAs; not reported to associatewith polyribosomes 0.006420.17009&radic S000003411OKP1YGR179CverifiedOuter kinetochore protein, required for accurate mitoticchromosome segregation; component of thekinetochore sub-complex COMA (Ctf19p, Okp1p,Mcm21p, Ame1p) that functions as a platform forkinetochore assembly 0.00630.16738&radic&radic S000005195NRD1YNL251CverifiedRNA-binding protein that interacts with the C-terminal domainof the RNA polymerase II large subunit(Rpo21p), required for transcriptiontermination and 3' end maturation ofnonpolyadenylated RNAs 0.006290.16738&radic&radic S000002687RNH202YDR279WverifiedRibonuclease H2 subunit, required for RNase H2 activity 0.006250.1666&radic&radic&radic S000004841MTF1YMR228WverifiedMitochondrial RNA polymerase specificity factor with structuralsimilarity to S-adenosylmethionine-dependentmethyltransferases and functional similarity tobacterial sigma-factors, interacts withmitochondrial core polymerase Rpo41p 0.006230.16563&radic&radic&radic S000004669KAR5YMR065WverifiedProtein required for nuclear membrane fusion during karyogamy,localizes to the membrane with a solubleportion in the endoplasmic reticulum lumen, mayform a complex with Jem1p and Kar2p; expressionof the gene is regulated by pheromone 0.006220.16563&radic&radic&radic S000005141WHI3YNL197CverifiedRNA binding protein that sequesters CLN3 mRNA in cytoplasmicfoci; cytoplasmic retention factor for Cdc28pand associated cyclins; regulates cell fate anddose-dependently regulates the critical cellsize required for passage through Start 0.006040.16123&radic&radic S000000364CDC28YBR160WverifiedCatalytic subunit of the main cell cycle cyclin-dependentkinase (CDK); alternately associates with G1cyclins (CLNs) and G2/M cyclins (CLBs) whichdirect the CDK to specific substrates 0.00610.16123&radic&radic&radic&radic S000005929CHL1YPL008WverifiedConserved nuclear protein required to establishsister-chromatid pairing during S-phase,probable DNA helicase with similarity to humanBACH1, which associates with tumor suppressorBRCA1; associates with acetyltransferase Ctf7p 0.006050.16123&radic S000005224ERG24YNL280CverifiedC-14 sterol reductase, acts in ergosterol biosynthesis; mutantsaccumulate the abnormal sterol ignosterol(ergosta-8,14 dienol), and are viable underanaerobic growth conditions but inviable onrich medium under aerobic conditions 0.006080.16123 S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.005990.16031&radic S000004284GSP1YLR293CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; regulated by Prp20p,Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p andKap95p; yeast Gsp2p homolog 0.005890.15796&radic&radic&radic S000004422SEN1YLR430WverifiedPutative helicase required for RNA polymerase II transcriptiontermination and processing of RNAs; homolog ofSenataxin which causes Ataxia-OculomotorApraxia 2 and a dominant form of amyotrophiclateral sclerosis 0.005870.1576&radic S000005504NOP8YOL144WverifiedNucleolar protein required for 60S ribosomal subunit biogenesis 0.005880.1576&radic S000004691YMR086C-ADubiousHypothetical protein 0.005860.15708&radic&radic S000003450CRM1YGR218WverifiedMajor karyopherin, involved in export of proteins, RNAs, andribosomal subunits from the nucleus 0.005840.15678&radic&radic S000005888PRT1YOR361CverifiedSubunit of the core complex of translation initiation factor3(eIF3), essential for translation; part of asubcomplex (Prt1p-Rpg1p-Nip1p) that stimulatesbinding of mRNA and tRNA(i)Met to ribosomes 0.005770.15445 S000001111RRP4YHR069CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp43p and Dis3p 0.005750.15445&radic&radic&radic S000001204YAP1801YHR161CverifiedProtein involved in clathrin cage assembly; binds Pan1p andclathrin; homologous to Yap1802p, member of theAP180 protein family 0.005730.15421 S000001016RIM4YHL024WverifiedPutative RNA-binding protein required for the expression ofearly and middle sporulation genes 0.005720.15383 S000000234YBR030WUncharacterizedHypothetical protein 0.005710.15364&radic S000000472MRPL37YBR268WverifiedMitochondrial ribosomal protein of the large subunit 0.005660.15272 S000001334HOP1YIL072WverifiedMeiosis-specific DNA binding protein that displays Red1pdependent localization to the unsynapsedaxial-lateral elements of the synaptonemalcomplex; required for homologous chromosomesynapsis and chiasma formation 0.005580.15009&radic&radic&radic S000003413TIM13YGR181WverifiedMitochondrial intermembrane space protein, forms a complex withTIm8p that mediates import and insertion of asubset of polytopic inner membrane proteins;may prevent aggregation of incoming proteins ina chaperone-like manner 0.005490.1479&radic&radic&radic&radic S000006105MRN1YPL184CuncharacterizedPutative RNA binding protein 0.005420.14592&radic S000003305YGR073CDubiousHypothetical protein; open reading frame extensively overlaps essential SMD1 gene encoding a U6 snRNP protein 0.00540.14592&radic&radic S000002157NHP6BYBR089C-AverifiedHigh-mobility group non-histone chromatin protein, functionallyredundant with Nhp6Ap; homologous to mammalianhigh mobility group proteins 1 and 2; acts torecruit transcription factor Rcs1p to certainpromoters 0.005330.14409&radic&radic&radic S000001566YKL083WDubiousDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 0.005330.14409&radic&radic&radic&radic S000002872SPP41YDR464WverifiedProtein involved in negative regulation of expression ofspliceosome components PRP4 and PRP3 0.00530.14322&radic&radic S000001745SPC34YKR037CverifiedEssential subunit of the Dam1 complex (aka DASH complex),couples kinetochores to the force produced byMT depolymerization thereby aiding inchromosome segregation; also localized tonuclear side of spindle pole body 0.005270.14244&radic&radic&radic S000005996GCR1YPL075WverifiedTranscriptional activator of genes involved in glycolysis;DNA-binding protein that interacts andfunctions with the transcriptional activatorGcr2p 0.005290.14244&radic&radic&radic&radic S000005483HRP1YOL123WverifiedSubunit of cleavage factor I, a five-subunit complex requiredfor the cleavage and polyadenylation ofpre-mRNA 3' ends; RRM-containing heteronuclearRNA binding protein and hnRNPA/B family memberthat binds to poly (A) signal sequences 0.005260.14218&radic&radic S000004980YNL035CUncharacterizedPutative protein of unknown function with similarity to proteins containing WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene 0.005250.14178&radic&radic S000001188YHR145CDubiousHypothetical protein 0.005240.14162&radic&radic&radic S000004759YIM2YMR151WdubiousDubious open reading frame, unlikely to encode a protein; notconserved in closely related Saccharomycesspecies; 5% of ORF overlaps the verified geneIMP1 0.005180.13984&radic S000003012RNA15YGL044CverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; interacts with the A-rich polyadenylationsignal in complex with Rna14p and Hrp1p 0.005140.13915&radic S000006316MRD1YPR112CverifiedEssential conserved protein that associates with 35S precursorrRNA and is required for its initial processingat the A(0)-A(2) cleavage sites, shows partialnucleolar localization, contains five consensusRNA-binding domains 0.005150.13915 S000004339KAP95YLR347CverifiedKaryopherin beta, forms a dimeric complex with Srp1p (Kap60p)that mediates nuclear import of cargo proteinsvia a nuclear localization signal (NLS),interacts with nucleoporins to guide transportacross the nuclear pore complex 0.005150.13915 S000003304UPF3YGR072WverifiedComponent of the nonsense-mediated mRNA decay (NMD) pathway,along with Nam7p and Nmd2p; involved in decayof mRNA containing nonsense codons; involved intelomere maintenance 0.00510.13718&radic&radic S000002326NRP1YDL167CverifiedProtein of unknown function, rich in asparagine residues 0.00510.13718&radic&radic S000000076YAR028WUncharacterizedPutative integral membrane protein, member of DUP240 gene family 0.00510.13718&radic&radic&radic S000004806MRPL24YMR193WverifiedMitochondrial ribosomal protein of the large subunit 0.005110.13718&radic S000002501YDR094WDubiousHypothetical protein 0.00510.13718 S000003414YGR182CDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W 0.005070.13667&radic S000004556RPM2YML091CverifiedProtein component of mitochondrial RNase P, along with themitochondrially-encoded RNA subunit RPM1; RnaseP removes 5' extensions from tRNA precursors;Rpm2p is also involved in maturation of RPM1and in translation of mitochondrial mRNAs 0.005030.13534 S000002653MNN10YDR245WverifiedSubunit of a Golgi mannosyltransferase complex also containingAnp1p, Mnn9p, Mnn11p, and Hoc1p that mediateselongation of the polysaccharide mannanbackbone; membrane protein of themannosyltransferase family 0.005010.13511&radic&radic&radic S000003142BUD13YGL174WverifiedSubunit of the RES complex, which is required for nuclearpre-mRNA retention and splicing; involved inbud-site selection; diploid mutants display aunipolar budding pattern instead of thewild-type bipolar pattern 0.005010.13433&radic&radic&radic S000002722RAD34YDR314CverifiedProtein involved in nucleotide excision repair (NER);homologous to RAD4 0.004960.13329&radic&radic&radic&radic S000000468YPT10YBR264CverifiedGTP binding protein that contains the PEST signal sequencespecific for proteolytic enzymes; may beinvolved in vesicular transport; overexpressionleads to accumulation of Golgi-like cisternaewith budding vesicles 0.004930.13197 S000000559SPB1YCL054WverifiedAdoMet-dependent methyltransferase involved in rRNA processingand 60S ribosomal subunit maturation;methylates G2922 in the tRNA docking site ofthe large subunit rRNA and in the absence ofsnR52, U2921; suppressor of PAB1 mutants 0.004880.13141&radic&radic&radic&radic S000004749YMR141CDubiousHypothetical protein 0.00490.13141&radic&radic&radic S000002270TRM3YDL112Wverified2'-O-ribose methyltransferase, catalyzes the ribose methylationof the guanosine nucleotide at position 18 oftRNAs 0.004870.13108&radic S000005442ATG19YOL082WverifiedProtein involved in the cytoplasm-to-vacuole targeting pathwayand in autophagy, recognizes cargo proteins anddelivers them to the preautophagosomalstructure for eventual engulfment by theautophagosome and degradation 0.004780.12744 S000003391NSR1YGR159CverifiedNucleolar protein that binds nuclear localization sequences,required for pre-rRNA processing and ribosomebiogenesis 0.004690.12576&radic&radic S000005054NOP15YNL110CverifiedConstituent of 66S pre-ribosomal particles, involved in 60Sribosomal subunit biogenesis; localizes to bothnucleolus and cytoplasm 0.004680.12515&radic&radic S000004657STB2YMR053CverifiedProtein that interacts with Sin3p in a two-hybrid assay and ispart of a large protein complex with Sin3p andStb1p 0.004680.12515 S000000744EAF5YEL018WuncharacterizedEsa1p-associated factor, subunit of the NuA4 acetyltransferasecomplex 0.004640.12381&radic&radic S000004125SLX4YLR135WverifiedSubunit of a complex, with Slx1p, that hydrolyzes 5' branchesfrom duplex DNA in response to stalled orconverging replication forks; function overlapswith that of Sgs1p-Top3p 0.004620.1232&radic S000006302YPR098CVerifiedProtein of unknown function, localized to the mitochondrial outer membrane 0.004610.12299 S000002254OPI6YDL096CdubiousDubious open reading frame unlikely to encode a protein, basedon available experimental and comparativesequence data; partially overlaps verified genePMT1/YDL095W; YDL096C is not essential 0.00460.12251&radic&radic&radic S000000416NGR1YBR212WverifiedRNA binding protein that negatively regulates growth rate;interacts with the 3' UTR of the mitochondrialporin (POR1) mRNA and enhances its degradation;overexpression impairs mitochondrial function;expressed in stationary phase 0.004580.12201&radic S000003440SER2YGR208WverifiedPhosphoserine phosphatase of the phosphoglycerate pathway,involved in serine and glycine biosynthesis,expression is regulated by the availablenitrogen source 0.004580.12201&radic&radic&radic S000005964NOP4YPL043WverifiedNucleolar protein, essential for processing and maturation of27S pre-rRNA and large ribosomal subunitbiogenesis; constituent of 66S pre-ribosomalparticles; contains four RNA recognition motifs(RRMs) 0.004570.12201&radic S000003884RPS5YJR123WverifiedProtein component of the small (40S) ribosomal subunit, theleast basic of the non-acidic ribosomalproteins; phosphorylated in vivo; essential forviability; has similarity to E. coli S7 and ratS5 ribosomal proteins 0.004560.12105&radic S000004675TVP18YMR071CverifiedIntegral membrane protein localized to late Golgi vesiclesalong with the v-SNARE Tlg2p 0.004560.12105&radic S000003428FYV8YGR196CverifiedProtein of unknown function, required for survival uponexposure to K1 killer toxin 0.004570.12105 S000006246PUF2YPR042CverifiedMember of the PUF protein family, which is defined by thepresence of Pumilio homology domains thatconfer RNA binding activity; preferentiallybinds mRNAs encoding membrane-associatedproteins 0.00450.11865 S000003361SYF2YGR129WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression &radic0.004470.11816&radic&radic&radic&radic S000001440SGN1YIR001CverifiedCytoplasmic RNA-binding protein, contains an RNA recognitionmotif (RRM); may have a role in mRNAtranslation, as suggested by geneticinteractions with genes encoding proteinsinvolved in translational initiation 0.004450.11776&radic S000002140YAL064C-AUncharacterizedHypothetical protein 0.004430.11721&radic&radic&radic S000001504MAK11YKL021CverifiedProtein essential for cell growth and replication of M dsRNAvirus; contains four beta-transducin repeats 0.004420.117&radic&radic&radic&radic&radic S000000673PAT1YCR077CverifiedTopoisomerase II-associated deadenylation-dependentmRNA-decapping factor; also required forfaithful chromosome transmission, maintenanceof rDNA locus stability, and protection of mRNA3'-UTRs from trimming; functionally linked toPab1p 0.004410.11665&radic S000002471RPS13YDR064WverifiedProtein component of the small (40S) ribosomal subunit; hassimilarity to E. coli S15 and rat S13 ribosomalproteins 0.00440.11643&radic&radic&radic S000001696DOA1YKL213CverifiedWD repeat protein required for ubiquitin-mediated proteindegradation, forms complex with Cdc48p, plays arole in controlling cellular ubiquitinconcentration; also promotes efficient NHEJ inpostdiauxic/stationary phase 0.004380.11546&radic S000005732NOC2YOR206WverifiedProtein that forms a nucleolar complex with Mak21p that bindsto 90S and 66S pre-ribosomes, as well as anuclear complex with Noc3p that binds to 66Spre-ribosomes; both complexes mediateintranuclear transport of ribosomal precursors 0.004390.11546&radic&radic S000001014SPO11YHL022CverifiedMeiosis-specific protein that initiates meiotic recombinationby catalyzing the formation of double-strandbreaks in DNA via a transesterificationreaction; required for homologous chromosomepairing and synaptonemal complex formation 0.004340.11417&radic&radic&radic S000002142PAU8YAL068CuncharacterizedHypothetical protein 0.004310.11313&radic&radic&radic S000006365SGV1YPR161CverifiedCyclin (Bur2p)-dependent protein kinase that functions intranscriptional regulation; phosphorylates thecarboxy-terminal domain of Rpo21p, which is thelargest subunit of RNA polymerase II; regulatedby Cak1p 0.004290.11219&radic&radic&radic S000001052RPL27AYHR010WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl27Bp and has similarity to ratL27 ribosomal protein 0.004290.11219&radic&radic&radic&radic&radic S000003271YGR039WDubiousHypothetical protein 0.004230.11066&radic&radic&radic&radic S000005263YNL319WDubiousDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 0.004210.10971&radic S000004410CDC73YLR418CverifiedConstituent of Paf1 complex with RNA polymerase II, Paf1p,Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinctfrom Srb-containing Pol II complexes; requiredfor expression of certain genes, modificationof some histones, and telomere maintenance 0.00420.10936&radic&radic&radic S000003969RNP1YLL046CverifiedRibonucleoprotein that contains two RNA recognition motifs(RRM) 0.004190.10908 S000000752SNU13YEL026WverifiedRNA binding protein, part of U3 snoRNP involved in rRNAprocessing, part of U4/U6-U5 tri-snRNP involvedin mRNA splicing, similar to human 15.5Kprotein &radic0.004150.10814&radic&radic S000004868GFD1YMR255WverifiedCoiled-coiled protein of unknown function, identified as ahigh-copy suppressor of a dbp5 mutation 0.004150.10814&radic S000003506TAF1YGR274CverifiedTFIID subunit (145 kDa), involved in RNA polymerase IItranscription initiation, has histoneacetyltransferase activity, involved inpromoter binding and G1/S progression 0.004110.10614&radic&radic&radic S000003700TPK1YJL164CverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; inhibits filamentous growth 0.004110.10614&radic&radic&radic S000001901SAD1YFR005CverifiedConserved zinc-finger domain protein involved in pre-mRNAsplicing, required for assembly of U4 snRNAinto the U4/U6 particle &radic0.004070.10507&radic&radic&radic&radic S000003335NOP7YGR103WverifiedNucleolar protein involved in rRNA processing and 60S ribosomalsubunit biogenesis; constituent of severaldifferent pre-ribosomal particles; required forexit from G0 and the initiation ofcell proliferation 0.004050.10489&radic&radic&radic S000000303YBR099CDubiousHypothetical protein 0.004050.10459&radic&radic S000002483RAD55YDR076WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad57p 0.004040.10432&radic&radic&radic S000004917YME2YMR302CverifiedIntegral inner mitochondrial membrane protein with similarityto exonucleases; mutants exhibit an increasedrate of mitochondrial DNA escape 0.004010.10321 S000002222UBC9YDL064WverifiedSUMO-conjugating enzyme involved in the Smt3p conjugationpathway; nuclear protein required for S- andM-phase cyclin degradation and mitotic control;involved in proteolysis mediated by theanaphase-promoting complex cyclosome (APCC) 0.003950.10036&radic&radic S000003141KEM1YGL173CverifiedEvolutionarily-conserved 5'-3' exonuclease component ofcytoplasmic processing (P) bodies involved inmRNA decay; plays a role inmicrotubule-mediated processes, filamentousgrowth, ribosomal RNA maturation, and telomeremaintenance 0.003890.09928 S000004045SPT8YLR055CverifiedSubunit of the SAGA transcriptional regulatory complex but notpresent in SAGA-like complex SLIK/SALSA,required for SAGA-mediated inhibition at somepromoters 0.003880.09869&radic&radic&radic S000000104HIR1YBL008WverifiedNon-essential transcriptional corepressor involved in the cellcycle-regulated transcription of histone H2A,H2B, H3 and H4 genes; contributes to nucleosomeformation, heterochromatic gene silencing, andformation of functional kinetochores 0.003870.09869&radic S000000167YBL071CDubiousHypothetical protein 0.003860.09707&radic&radic&radic S000002251PMT5YDL093WverifiedProtein O-mannosyltransferase, transfers mannose residues fromdolichyl phosphate-D-mannose to proteinserine/threonine residues; acts in a complexwith Pmt3p, can instead interact with Pmt2p insome conditions; target for new antifungals 0.003830.09707&radic&radic&radic S000001652YKL169CDubiousHypothetical protein 0.003830.09671&radic&radic&radic S000003207CSE1YGL238WverifiedNuclear envelope protein that mediates the nuclear export ofimportin alpha (Srp1p), homolog of metazoan CASprotein, required for accurate chromosomesegregation 0.003810.09624&radic&radic&radic&radic S000005784HNT3YOR258WverifiedMember of the third branch of the histidine triad (HIT)superfamily of nucleotide-binding proteins;similar to Aprataxin, a Hint related proteinthat is mutated in individuals with ataxia withoculomotor apraxia 0.003810.09624&radic&radic&radic&radic&radic S000002271ATG20YDL113CverifiedProtein required for transport of aminopeptidase I (Lap4p)through the cytoplasm-to-vacuole targetingpathway; bindsphosphatidylinositol-3-phosphate, involved inlocalization of membranes to thepreautophagosome, potential Cdc28p substrate 0.003770.09479&radic S000004650NUP116YMR047CverifiedSubunit of the nuclear pore complex (NPC) that is localized toboth sides of the pore; contains a repetitiveGLFG motif that interacts with mRNA exportfactor Mex67p and with karyopherin Kap95p;homologous to Nup100p 0.003740.09384&radic&radic S000000613PET18YCR020CverifiedProtein required for respiratory growth and stability of themitochondrial genome 0.003730.09314&radic&radic S000001928YFR032CUncharacterizedPutative protein of unknown function; transcribed during sporulation; YFR032C is not an essential gene 0.003720.09265 S000005509DCP1YOL149WverifiedSubunit of the Dcp1p-Dcp2p decapping enzyme complex, whichremoves the 5' cap structure from mRNAs priorto their degradation; enhances the activity ofcatalytic subunit Dcp2p; regulated by DEAD boxprotein Dhh1p 0.003710.09265&radic&radic&radic S000006367TIF3YPR163CverifiedTranslation initiation factor eIF-4B, has RNA annealingactivity; contains an RNA recognition motif andbinds to single-stranded RNA 0.003710.09265&radic S000004043YLR053CUncharacterizedHypothetical protein 0.003720.09265&radic&radic S000000025YAL027WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YAL027W is a non-essential gene 0.00370.09218&radic&radic&radic&radic S000003272KSS1YGR040WverifiedMitogen-activated protein kinase (MAPK) involved in signaltransduction pathways that control filamentousgrowth and pheromone response 0.00370.09218&radic&radic&radic&radic S000000682CSM1YCR086WverifiedNucleolar protein that forms a complex with Lrs4p which bindsMam1p at kinetochores during meiosis I tomediate accurate chromosome segregation, may beinvolved in premeiotic DNA replication;possible role in telomere maintenance 0.003660.09084&radic&radic&radic&radic&radic S000004018ADE16YLR028CverifiedEnzyme of 'de novo' purine biosynthesis containing both5-aminoimidazole-4-carboxamide ribonucleotidetransformylase and inosine monophosphatecyclohydrolase activities, isozyme of Ade17p;ade16 ade17 mutants require adenine andhistidine 0.003630.0896 S000000392NTC20YBR188CverifiedMember of a complex, including Prp19p, that binds to thespliceosome; required for pre-mRNA splicing &radic0.003620.08948&radic&radic&radic S000002774YDR366CUncharacterizedHypothetical protein 0.003610.08925 S000003521MAL11YGR289CverifiedMaltose permease, inducible high-affinity maltose transporter(alpha-glucoside transporter); encoded in theMAL1 complex locus; member of the 12transmembrane domain superfamily of sugartransporters 0.003610.08925&radic&radic S000006341RRP9YPR137WverifiedProtein involved in pre-rRNA processing, associated with U3snRNP; component of small ribosomal subunit(SSU) processosome; ortholog of the humanU3-55k protein 0.003620.08925&radic&radic S000004280GUF1YLR289WverifiedMitochondrial GTPase of unknown function, similar to E. colielongation factor-type GTP-binding protein LepAand to LK1236.1 from Caenorhabditis elegans 0.003590.08791&radic&radic S000001643ELF1YKL160WverifiedTranscription elongation factor that contains a conserved zincfinger domain; implicated in the maintenance ofproper chromatin structure in activelytranscribed regions; deletion inhibits Bromemosaic virus (BMV) gene expression 0.003580.08791&radic&radic&radic&radic&radic S000004134ACF2YLR144CverifiedIntracellular beta-1,3-endoglucanase, expression is inducedduring sporulation; may have a role in corticalactin cytoskeleton assembly 0.003580.08791&radic&radic S000003295SPT4YGR063CverifiedProtein that forms a complex with Spt5p and mediates bothactivation and inhibition of transcriptionelongation, and plays a role in pre-mRNAprocessing; in addition, Spt4p is involved inkinetochore function and gene silencing 0.003570.08749&radic S000002241RPS16BYDL083CverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps16Ap and has similarity to E.coli S9 and rat S16 ribosomal proteins 0.003560.0872&radic S000005808YOR282WDubiousHypothetical protein; open reading frame overlaps 5' end of essential PLP2 gene 0.003530.08608&radic&radic S000002665SET7YDR257CverifiedNuclear protein that contains a SET-domain, which have beenshown to mediate methyltransferase activity inother proteins 0.003520.08597&radic&radic&radic&radic&radic S000005261PFS2YNL317WverifiedIntegral subunit of the pre-mRNA cleavage and polyadenylationfactor (CPF) complex; plays an essential rolein mRNA 3'-end formation by bridging differentprocessing factors and thereby promoting theassembly of the processing complex 0.003510.08537&radic&radic S000003085YGL117WUncharacterizedPutative protein of unknown function 0.003510.08537&radic&radic&radic S000003193VRG4YGL225WverifiedGolgi GDP-mannose transporter; regulates Golgi function andglycosylation in Golgi 0.003450.08279&radic&radic S000001335SPO22YIL073CverifiedMeiosis-specific protein essential for chromosome synapsis,similar to phospholipase A2, involved incompletion of nuclear divisions during meiosis;induced early in meiosis 0.003470.08279&radic&radic&radic S000003213RTF1YGL244WverifiedSubunit of the RNA polymerase II-associated Paf1 complex;directly or indirectly regulates DNA-bindingproperties of Spt15p and relative activities ofdifferent TATA elements; involved in telomeremaintenance 0.003450.08279&radic&radic&radic S000000653PWP2YCR057CverifiedConserved 90S pre-ribosomal component essential for properendonucleolytic cleavage of the 35 S rRNAprecursor at A0, A1, and A2 sites; containseight WD-repeats; PWP2 deletion leads todefects in cell cycle and bud morphogenesis 0.003440.08279 S000005334BRE5YNR051CverifiedUbiquitin protease cofactor, forms deubiquitination complexwith Ubp3p that coregulates anterograde andretrograde transport between the endoplasmicreticulum and Golgi compartments; null issensitive to brefeldin A 0.003430.08256 S000000200YBL104CUncharacterizedHypothetical protein 0.003410.08177 S000004255NEJ1YLR265CverifiedProtein involved in regulation of nonhomologous end joining;repressed by MAT heterozygosity; associateswith Lif1p and regulates its cellulardistribution 0.003420.08177&radic&radic&radic&radic&radic S000002636PCF11YDR228CverifiedmRNA 3' end processing factor, essential component of cleavageand polyadenylation factor IA (CF IA), involvedin pre-mRNA 3' end processing and intranscription termination; binds C-terminaldomain of largest subunit of RNA pol II(Rpo21p) 0.003390.08113&radic&radic&radic&radic S000000973RAD3YER171Wverified5' to 3' DNA helicase, involved in nucleotide excision repairand transcription; subunit of RNA polymerase IItranscription initiation factor TFIIH; subunitof Nucleotide Excision Repair Factor 3 (NEF3);homolog of human XPD protein 0.003360.08027&radic S000005189CWC25YNL245CverifiedComponent of a complex containing Cef1p, involved in pre-mRNAsplicing; has similarity to S. pombe Cwf25p 0.003330.0786&radic&radic&radic S000002604YDR196CUncharacterizedpredicted to catalyze the final step in synthesis of Coenzyme A 0.003330.0786&radic S000000302MMS4YBR098WverifiedSubunit of the structure-specific Mms4p-Mus81p endonucleasethat cleaves branched DNA; involved inrecombination and DNA repair 0.003320.0786&radic&radic&radic&radic&radic S000000325GRS1YBR121CverifiedCytoplasmic and mitochondrial glycyl-tRNA synthase that ligatesglycine to the cognate anticodon bearing tRNA;transcription termination factor that mayinteract with the 3'-end of pre-mRNA to promote3'-end formation 0.003310.07829&radic S000002198NAT1YDL040CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.003290.07761&radic&radic S000004064BUD20YLR074CverifiedProtein involved in bud-site selection; diploid mutants displaya random budding pattern instead of thewild-type bipolar pattern 0.00330.07761&radic&radic S000001156BZZ1YHR114WverifiedSH3 domain protein implicated in the regulation of actinpolymerization, able to recruit actinpolymerization machinery through its SH3domains, colocalizes with cortical actinpatches and Las17p, interacts with type Imyosins 0.003240.07597&radic S000003693FAR1YJL157CverifiedCyclin-dependent kinase inhibitor that mediates cell cyclearrest in response to pheromone; also forms acomplex with Cdc24p, Ste4p, and Ste18p that mayspecify the direction of polarized growthduring mating; potential Cdc28p substrate 0.003210.07512&radic&radic&radic&radic S000004006PML1YLR016CverifiedSubunit of the RES complex, which is required for nuclearretention of unspliced pre-mRNAs; acts in thesame pathway as Pml39p and Mlp1p 0.00320.07428&radic&radic S000004352VPS38YLR360WverifiedPart of a Vps34p phosphatidylinositol 3-kinase complex thatfunctions in carboxypeptidase Y (CPY) sorting;binds Vps30p and Vps34p to promote productionof phosphatidylinositol 3-phosphate (PtdIns3P)which stimulates kinase activity 0.00320.07428&radic&radic&radic&radic S000002383WHI4YDL224CverifiedPutative RNA binding protein and partially redundant Whi3phomolog that regulates the cell sizerequirement for passage through Start andcommitment to cell division 0.00320.07428&radic S000003684RPA34YJL148WverifiedRNA polymerase I subunit A34.5 0.00320.07428&radic&radic&radic S000000560KAR4YCL055WverifiedTranscription factor required for gene regulation in repsonseto pheromones; also required during meiosis;exists in two forms, a slower-migrating formmore abundant during vegetative growth and afaster-migrating form induced by pheromone 0.003190.07428&radic&radic&radic&radic&radic S000006132NIP7YPL211WverifiedNucleolar protein required for 60S ribosome subunit biogenesis,constituent of 66S pre-ribosomal particles;physically interacts with Nop8p and the exosomesubunit Rrp43p 0.003180.07428&radic&radic&radic S000000633PHO87YCR037CverifiedLow-affinity inorganic phosphate (Pi) transporter, involved inactivation of PHO pathway; expression isindependent of Pi concentration and Pho4pactivity; contains 12 membrane-spanningsegments 0.003160.07357&radic&radic&radic S000002601YDR193WDubiousHypothetical protein 0.003170.07357&radic&radic&radic S000000667IMG2YCR071CverifiedMitochondrial ribosomal protein of the small subunit 0.003180.07357&radic&radic&radic&radic&radic S000004486RPS17AYML024WverifiedRibosomal protein 51 (rp51) of the small (40s) subunit; nearlyidentical to Rps17Bp and has similarity to ratS17 ribosomal protein 0.003160.07357 S000001029YHL037CDubiousHypothetical protein 0.003130.07235&radic&radic S000003188YGL220WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus 0.003160.07235&radic&radic S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.003140.07235&radic&radic&radic S000001949HXK1YFR053CverifiedHexokinase isoenzyme 1, a cytosolic protein that catalyzesphosphorylation of glucose during glucosemetabolism; expression is highest during growthon non-glucose carbon sources; glucose-inducedrepression involves the hexokinase Hxk2p 0.003130.07235 S000003993YLR003CUncharacterizedPutative protein of unknown function that may participate in DNA replication; green fluorescent protein (GFP)-fusion protein is localized to the nucleus; YLR003C is not an essential gene 0.003140.07235&radic&radic S000004433RPS1AYLR441CverifiedRibosomal protein 10 (rp10) of the small (40S) subunit; nearlyidentical to Rps1Bp and has similarity to ratS3a ribosomal protein 0.003110.07126&radic&radic&radic S000002232BRE1YDL074CverifiedE3 ubiquitin ligase for Rad6p, required for the ubiquitinationof histone H2B, recruitment of Rad6p topromoter chromatin and subsequent methylationof histone H3 (on L4 and L79), contains RINGfinger domain 0.003120.07126&radic&radic S000002773ESF1YDR365CverifiedNucleolar protein involved in pre-rRNA processing; depletioncauses severely decreased 18S rRNA levels 0.00310.07126&radic&radic&radic&radic S000001051YHR009CUncharacterizedPutative protein of unknown function; not an essential gene 0.00310.07126&radic&radic&radic&radic&radic&radic S000002214MBP1YDL056WverifiedTranscription factor involved in regulation of cell cycleprogression from G1 to S phase, forms a complexwith Swi6p that binds to MluI cell cycle boxregulatory element in promoters of DNAsynthesis genes 0.003080.07047&radic&radic S000006050TAF14YPL129WverifiedSubunit (30 kDa) of TFIID, TFIIF, and SWI/SNF complexes,involved in RNA polymerase II transcriptioninitiation and in chromatin modification,contains a YEATS domain 0.003070.07032&radic&radic S000002922YDR514CUncharacterizedHypothetical protein 0.003050.06956&radic&radic S000001215SPC97YHR172WverifiedComponent of the microtubule-nucleating Tub4p (gamma-tubulin)complex; interacts with Spc110p at the spindlepole body (SPB) inner plaque and with Spc72p atthe SPB outer plaque 0.003060.06956&radic&radic&radic S000006339CTF4YPR135WverifiedChromatin-associated protein, required for sister chromatidcohesion; interacts with DNA polymerase alpha(Pol1p) and may link DNA synthesis to sisterchromatid cohesion 0.003050.06956&radic S000005190VPS75YNL246WverifiedProtein of unknown function involved in vacuolar proteinsorting; detected in the nucleus 0.003040.06925&radic&radic&radic S000000003EFB1YAL003WverifiedTranslation elongation factor 1 beta; stimulates nucleotideexchange to regenerate EF-1 alpha-GTP for thenext elongation cycle; part of the EF-1complex, which facilitates binding ofaminoacyl-tRNA to the ribosomal A site 0.003040.069&radic&radic S000002312SAS10YDL153CverifiedComponent of the small (ribosomal) subunit (SSU) processosomerequired for pre-18S rRNa processing; essentialnucleolar protein that, when overproduced,disrupts silencing 0.003020.06847&radic&radic&radic S000004995YNL050CUncharacterizedPutative protein of unknown function; YNL050c is not an essential gene 0.003010.06808&radic&radic&radic&radic S000004279MEC3YLR288CverifiedDNA damage and meiotic pachytene checkpoint protein; subunit ofa heterotrimeric complex (Rad17p-Mec3p-Ddc1p)that forms a sliding clamp, loaded onto partialduplex DNA by a clamp loader complex; homologof human and S. pombe Hus1 0.0030.06782&radic&radic S000000173YBL077WDubiousHypothetical protein; open reading frame overlaps 3' end of essential ILS1 gene encoding cytoplasmic isoleucine-tRNA synthetase 0.0030.06782&radic&radic&radic S000000813TIR1YER011WverifiedCell wall mannoprotein of the Srp1p/Tip1p family ofserine-alanine-rich proteins; expression isdownregulated at acidic pH and induced by coldshock and anaerobiosis; abundance is increasedin cells cultured without shaking 0.002990.06715 S000001730NTR2YKR022CverifiedEssential protein that forms a dimer with Ntr1p; also forms atrimer, with Ntr2p and the DExD/H-box RNAhelicase Prp43p, that is involved inspliceosome disassembly 0.002980.06715&radic&radic S000003427SKI6YGR195Wverified3'-to-5' phosphorolytic exoribonuclease that is a subunit ofthe exosome; required for 3' processing of the5.8S rRNA; involved in 3' to 5' mRNAdegradation and translation inhibition ofnon-poly(A) mRNAs 0.002970.06686&radic S000005584ASE1YOR058CverifiedMember of a family of microtubule-associated proteins (MAPs)that function at the mitotic spindle midzone;required for spindle elongation; undergoes cellcycle-regulated degradation by anaphasepromoting complex; potential Cdc28p substrate 0.002980.06686&radic S000002474OCA6YDR067CuncharacterizedCytoplasmic protein required for replication of Brome mosaicvirus in S. cerevisiae, which is a model systemfor studying replication of positive-strand RNAviruses in their natural hosts 0.002970.06686&radic&radic S000001954MEI4YER044C-AverifiedMeiosis-specific protein involved in recombination; requiredfor chromosome synapsis; required forproduction of viable spores 0.002960.06617&radic S000004527MFT1YML062CverifiedSubunit of the THO complex, which is a nuclear complexcomprised of Hpr1p, Mft1p, Rlr1p, and Thp2p,that is involved in transcription elongationand mitotic recombination; involved in telomeremaintenance 0.002950.06587&radic&radic&radic S000003849BIR1YJR089WverifiedEssential chromosomal passenger protein involved incoordinating cell cycle events for properchromosome segregation; C-terminal region bindsSli15p, and the middle region, uponphosphorylation, localizes Cbf2p to the spindleat anaphase 0.002940.06587&radic&radic&radic&radic S000004632RSF1YMR030WverifiedProtein required for respiratory growth; localized to both thenucleus and mitochondrion; mutant displaysdecreased transcription of specific nuclear andmitochondrial genes whose products are involvedin respiratory growth 0.002930.06539&radic&radic&radic&radic S000005674SPP2YOR148CverifiedEssential protein that promotes the first step of splicing andis required for the final stages of spliceosomematuration; interacts with Prp2p, which mayrelease Spp2p from the spliceosome followingthe first cleavage reaction 0.002910.06475&radic&radic S000005985CWC27YPL064CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to S. pombeCwf27p 0.002890.06426&radic&radic S000004023RSC58YLR033WverifiedRemodels the structure of chromatin complex 58KDa subunit;Chromatin Remodeling Complex subunit 0.002890.06426&radic&radic&radic S000002470YDR063WUncharacterizedHypothetical protein 0.002880.06386&radic&radic&radic S000004131RRN5YLR141WverifiedProtein involved in transcription of rDNA by RNA polymerase I;transcription factor, member of UAF (upstreamactivation factor) family along with Rrn9p andRrn10p 0.002890.06386&radic&radic S000004538RPL6AYML073CverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, has similarity to Rpl6Bp andto rat L6 ribosomal protein; binds to 5.8S rRNA 0.002880.06386&radic S000000683YCR087WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene 0.002870.0636&radic&radic&radic&radic&radic S000000636MATALPHA1YCR040WverifiedTranscriptional co-activator involved in regulation ofmating-type-specific gene expression; targetsthe transcription factor Mcm1p to the promotersof alpha-specific genes; one of two genesencoded by the MATalpha mating type cassette 0.002870.0636&radic&radic&radic S000005176CSL4YNL232WverifiedSubunit of the exosome, which is an essential complex presentin both nucleus and cytoplasm that mediates RNAprocessing and degradation 0.002860.06301&radic&radic&radic S000005622RPS7AYOR096WverifiedProtein component of the small (40S) ribosomal subunit, nearlyidentical to Rps7Bp; interacts with Kti11p;deletion causes hypersensitivity to zymocin;has similarity to rat S7 and Xenopus S8ribosomal proteins 0.002860.06301&radic S000004872YMR259CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR259C is not an essential gene 0.002850.06281&radic&radic S000004814RAD14YMR201CverifiedProtein that recognizes and binds damaged DNA during nucleotideexcision repair; subunit of Nucleotide ExcisionRepair Factor 1 (NEF1); contains zinc fingermotif; homolog of human XPA protein 0.002850.06281&radic&radic S000002923SLF1YDR515WverifiedRNA binding protein that associates with polysomes; proposed tobe involved in regulating mRNA translation;involved in the copper-dependent mineralizationof copper sulfide complexes on cell surface incells cultured in copper salts 0.002850.06281&radic S000002558CTH1YDR151CverifiedMember of the CCCH zinc finger family; has similarity tomammalian Tis11 protein, which activatestranscription and also has a role in mRNAdegradation; may function with Tis11p in ironhomeostasis 0.002850.06281&radic&radic S000005735NPT1YOR209CverifiedNicotinate phosphoribosyltransferase, acts in the salvagepathway of NAD+ biosynthesis; required forsilencing at rDNA and telomeres and has a rolein silencing at mating-type loci; localized tothe nucleus 0.002830.06213&radic S000001919PES4YFR023WverifiedPoly(A) binding protein, suppressor of DNA polymerase epsilonmutation, similar to Mip6p 0.002820.06184&radic S000000393RPS9BYBR189WverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps9Ap and has similarity to E.coli S4 and rat S9 ribosomal proteins 0.002810.06056&radic&radic S000003084CDC20YGL116WverifiedCell-cycle regulated activator of anaphase-promotingcomplex/cyclosome (APC/C), which is requiredfor metaphase/anaphase transition; directsubiquitination of mitotic cyclins, Pds1p, andother anaphase inhibitors; potential Cdc28psubstrate 0.00280.06056&radic&radic&radic S000000230ETR1YBR026Cverified2-enoyl thioester reductase, member of the medium chaindehydrogenase/reductase family; localized to inmitochondria, where it has a probable role infatty acid synthesis 0.002780.06035&radic&radic&radic&radic S000006384AOS1YPR180WverifiedNuclear protein that acts as a heterodimer with Uba2p toactivate Smt3p (SUMO) before its conjugation toproteins (sumoylation), which may play a rolein protein targeting; essential for viability 0.002780.06035&radic&radic&radic&radic S000004172SWI6YLR182WverifiedTranscription cofactor, forms complexes with DNA-bindingproteins Swi4p and Mbp1p to regulatetranscription at the G1/S transition; involvedin meiotic gene expression; localizationregulated by phosphorylation; potential Cdc28psubstrate 0.002780.06035&radic&radic&radic S000002520PDS1YDR113CverifiedSecurin that inhibits anaphase by binding separin Esp1p, alsoblocks cyclin destruction and mitotic exit,essential for cell cycle arrest in mitosis inthe presence of DNA damage or aberrant mitoticspindles; also present in meiotic nuclei 0.002770.05994&radic&radic S000003879ILM1YJR118CverifiedProtein of unknown function; may be involved in mitochondrialDNA maintenance; required for slowed DNAsynthesis-induced filamentous growth 0.002760.05982&radic&radic S000002175CDC7YDL017WverifiedDDK (Dbf4-dependent kinase) catalytic subunit required forfiring origins and replication fork progressionin mitosis through phosphorylation of Mcm2-7pcomplexes and Cdc45p; kinase activitycorrelates with cyclical DBF4 expression 0.002760.05962&radic&radic&radic S000000692HMRA2YCR096CverifiedSilenced copy of a2 at HMR; similarity to Alpha2p; requiredalong with a1p for inhibiting expression of theHO endonuclease in a/alpha HO/HO diploid cellswith an active mating-type interconversionsystem 0.002750.05935&radic&radic S000004494RAD52YML032CverifiedProtein that stimulates strand exchange by facilitating Rad51pbinding to single-stranded DNA; annealscomplementary single-stranded DNA; involved inthe repair of double-strand breaks in DNAduring vegetative growth and meiosis 0.002750.05935&radic&radic S000005185ZWF1YNL241CverifiedGlucose-6-phosphate dehydrogenase (G6PD), catalyzes the firststep of the pentose phosphate pathway; involvedin adapting to oxidatve stress; homolog of thehuman G6PD which is deficient in patients withhemolytic anemia 0.002750.05935 S000004424IMD3YLR432WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.002740.05927 S000001891MSH4YFL003CverifiedProtein involved in meiotic recombination, required for normallevels of crossing over, colocalizes with Zip2pto discrete foci on meiotic chromosomes, hashomology to bacterial MutS protein 0.002740.05913&radic S000000381EHT1YBR177CverifiedAcyl-coenzymeA:ethanol O-acyltransferase that plays a minorrole in medium-chain fatty acid ethyl esterbiosynthesis; contains esterase activity;localizes to lipid particles and themitochondrial outer membrane 0.002730.05886&radic&radic S000002449YDR042CUncharacterizedHypothetical protein 0.002730.05886&radic&radic&radic&radic S000001942CNN1YFR046CverifiedKinetochore protein of unknown function; associated with theessential kinetochore proteins Nnf1p andSpc24p; phosphorylated by both Clb5-Cdk1 and,to a lesser extent, Clb2-Cdk1. 0.002740.05886&radic&radic S000004636ARP9YMR033WverifiedActin-related protein involved in transcriptional regulation;subunit of the chromatin remodeling Snf/Swicomplex 0.002730.05826&radic&radic S000002892VPS52YDR484WverifiedComponent of the GARP (Golgi-associated retrograde protein)complex, Vps51p-Vps52p-Vps53p-Vps54p, which isrequired for the recycling of proteins fromendosomes to the late Golgi; involved inlocalization of actin and chitin 0.002720.05826&radic&radic&radic S000001501SWD2YKL018WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lys 4 and is involved intelomeric silencing; subunit of CPF (cleavageand polyadenylation factor), a complex involvedin RNAP II transcription termination 0.002730.05826 S000000231YBR027CDubiousHypothetical protein 0.002720.05826&radic&radic&radic&radic S000001532CSE4YKL049CverifiedCentromere protein that resembles histones, required for properkinetochore function; homolog of human CENP-A 0.002720.05826&radic S000005080EAF7YNL136WverifiedSubunit of the NuA4 histone acetyltransferase complex, whichacetylates the N-terminal tails of histones H4and H2A 0.002690.05747&radic&radic&radic S000005403NTG2YOL043CverifiedDNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involvedin base excision repair, localizes to thenucleus 0.00270.05747&radic&radic S000005782TRE2YOR256CverifiedProtein of unknown function with similarity to TRE1/YPL176C;required for cell viability 0.00270.05747&radic&radic&radic S000003934SOF1YLL011WverifiedEssential subunit of the U3 (box C+D) snRNP complex requiredfor 2' O-methylation of pre-RNA; has similarityto the beta subunit of trimeric G-proteins andthe splicing factor Prp4p 0.002690.05747&radic S000001207DNA2YHR164CverifiedEssential tripartite DNA replication factor withsingle-stranded DNA-dependent ATPase,ATP-dependent nuclease, and helicaseactivities; required for Okazaki fragmentprocessing; involved in DNA repair pathways;potential Cdc28p substrate 0.00270.05747&radic&radic S000002314CLB3YDL155WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the G2/M transition; may beinvolved in DNA replication and spindleassembly; accumulates during S phase and G2,then targeted for ubiquitin-mediateddegradation 0.002680.05709&radic&radic&radic S000004751RPS16AYMR143WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps16Bp and has similarity to E.coli S9 and rat S16 ribosomal proteins 0.002670.05669 S000004876SAP30YMR263WverifiedSubunit of a histone deacetylase complex, along with Rpd3p andSin3p, that is involved in silencing attelomeres, rDNA, and silent mating-type loci;involved in telomere maintenance 0.002670.05669&radic&radic&radic S000003439YGR207CVerifiedProtein of unknown function; homolog of mammalian electron transfer flavoprotein complex subunit ETF-beta; interacts with frataxin (Yfh1p) 0.002670.05662&radic&radic S000004364SUR4YLR372WverifiedElongase, involved in fatty acid and sphingolipid biosynthesis;synthesizes very long chain 20-26-carbon fattyacids from C18-CoA primers; involved inregulation of sphingolipid biosynthesis 0.002670.05662 S000005355HXT17YNR072WverifiedHexose transporter, up-regulated in media containing raffinoseand galactose at pH 7.7 versus pH 4.7,repressed by high levels of glucose 0.002670.05644&radic&radic S000004621STB4YMR019WverifiedProtein that binds Sin3p in a two-hybrid assay; contains aZn(II)2Cys6 zinc finger domain characteristicof DNA-binding proteins 0.002650.05601&radic&radic&radic S000002996SCW11YGL028CverifiedCell wall protein with similarity to glucanases; may play arole in conjugation during mating based on itsregulation by Ste12p 0.002660.05601&radic&radic S000001108SSF1YHR066WverifiedConstituent of 66S pre-ribosomal particles, required forribosomal large subunit maturation;functionally redundant with Ssf2p; member ofthe Brix family 0.002660.05601&radic&radic&radic S000001635GPM1YKL152CverifiedTetrameric phosphoglycerate mutase, mediates the conversion of3-phosphoglycerate to 2-phosphoglycerate duringglycolysis and the reverse reaction duringgluconeogenesis 0.002640.05555&radic&radic S000003100YGL132WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C 0.002650.05555&radic&radic&radic&radic S000001318VHR1YIL056WuncharacterizedTranscriptional activator, required for the vitaminH-responsive element (VHRE) mediated inductionof VHT1 (Vitamin H transporter) and BIO5(biotin biosynthesis intermediate transporter)in response to low biotin concentrations 0.002620.05486&radic&radic S000004223EST1YLR233CverifiedTLC1 RNA-associated factor involved in telomere lengthregulation as the recruitment subunit of thetelomerase holoenzyme, has a possible role inactivating Est2p-TLC1-RNA bound to the telomere 0.002630.05486&radic&radic S000006322YPR118WVerifiedMethylthioribose-1-phosphate isomerase, catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway 0.002620.05486&radic&radic&radic&radic&radic&radic S000004550TUB1YML085CverifiedAlpha-tubulin; associates with beta-tubulin (Tub2p) to formtubulin dimer, which polymerizes to formmicrotubules 0.002620.05468&radic&radic S000000339CKS1YBR135WverifiedSubunit of the Cdc28 protein kinase, required for mitoticproteolysis, may also be involved in theproteolysis of the G1 cyclins 0.002620.05468&radic&radic S000002435REG1YDR028CverifiedRegulatory subunit of type 1 protein phosphatase Glc7p,involved in negative regulation ofglucose-repressible genes 0.002610.05406&radic S000000134MRPL16YBL038WverifiedMitochondrial ribosomal protein of the large subunit 0.002610.05406&radic&radic S000003337VMA21YGR105WverifiedIntegral membrane protein that is required for vacuolarH+-ATPase (V-ATPase) function, although not anactual component of the V-ATPase complex;functions in the assembly of the V-ATPase;localized to the yeast endoplasmic reticulum(ER) 0.002610.05406&radic&radic S000002570CWC15YDR163WverifiedNon-essential protein involved in pre-mRNA splicing, componentof a complex containing Cef1p; has similarityto S. pombe Cwf15p 0.002590.05381&radic S000005798YTM1YOR272WverifiedConstituent of 66S pre-ribosomal particles, required formaturation of the large ribosomal subunit 0.002590.05381&radic&radic&radic S000004758IMP1YMR150CverifiedCatalytic subunit of the mitochondrial inner membrane peptidasecomplex, required for maturation ofmitochondrial proteins of the intermembranespace; complex contains Imp1p and Imp2p (bothcatalytic subunits), and Som1p 0.00260.05381&radic&radic S000004119DIP2YLR129WverifiedNucleolar protein, specifically associated with the U3 snoRNA,part of the large ribonucleoprotein complexknown as the small subunit (SSU) processome,required for 18S rRNA biogenesis, part of theactive pre-rRNA processing complex 0.002570.05274&radic S000000372PEX32YBR168WverifiedPeroxisomal integral membrane protein, involved in negativeregulation of peroxisome size; partiallyfunctionally redundant with Pex31p; geneticinteractions suggest action at a stepdownstream of steps mediated by Pex28p andPex29p 0.002590.05274&radic&radic S000005795PAC1YOR269WverifiedProtein involved in nuclear migration, part of thedynein/dynactin pathway; targets dynein tomicrotubule tips, which is necessary forsliding of microtubules along bud cortex;synthetic lethal with bni1; homolog of humanLIS1 0.002580.05274&radic S000006402SGE1YPR198WverifiedMembrane-associated multidrug transporter, acts as an extrusionpermease, member of the drug-resistance proteinfamily within the major facilitator superfamily(MFS), partial multicopy suppressor of gal11mutations 0.002580.05274&radic&radic S000001258PHO12YHR215WverifiedOne of three repressible acid phosphatases, a glycoprotein thatis transported to the cell surface by thesecretory pathway; nearly identical to Pho11p;upregulated by phosphate starvation 0.002590.05274&radic&radic S000004186PWP1YLR196WverifiedProtein with WD-40 repeats involved in rRNA processing;associates with trans-acting ribosomebiogenesis factors; similar to beta-transducinsuperfamily 0.002590.05274&radic S000004117APC2YLR127CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; similar tocullin Cdc53p 0.002590.05274&radic&radic&radic S000000913SWI4YER111CverifiedDNA binding component of the SBF complex (Swi4p-Swi6p), atranscriptional activator that in concert withMBF (Mbp1-Swi6p) regulates late G1-specifictranscription of targets including cyclins andgenes required for DNA synthesis and repair 0.002560.05259&radic S000004333SPO77YLR341WverifiedMeiosis-specific protein of unknown function, required forspore wall formation during sporulation;dispensable for both nuclear divisions duringmeiosis 0.002560.05259&radic S000002388SSB1YDL229WverifiedCytoplasmic ATPase that is a ribosome-associated molecularchaperone, functions with J-protein partnerZuo1p; may be involved in folding of newly-madepolypeptide chains; member of the HSP70 family;interacts with phosphatase subunit Reg1p 0.002560.05226&radic S000005682NFI1YOR156CverifiedSUMO ligase, catalyzes the covalent attachment of SUMO (Smt3p)to proteins; involved in maintenance of propertelomere length 0.002550.05226&radic&radic&radic&radic S000003588TDH1YJL052WverifiedGlyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involvedin glycolysis and gluconeogenesis; tetramerthat catalyzes the reaction ofglyceraldehyde-3-phosphate to 1,3bis-phosphoglycerate; detected in the cytoplasmand cell-wall 0.002540.05159&radic S000002311YDL152WDubiousHypothetical protein; open reading frame overlaps 5' end of essential SAS10 gene encoding a component of the small ribosomal subunit processosome 0.002550.05159&radic&radic&radic&radic S000001562SMY1YKL079WverifiedProtein that interacts with Myo2p, proposed to be involved inexocytosis; N-terminal domain is related to themotor domain of kinesins 0.002550.05159&radic&radic&radic S000001882YFL012WUncharacterizedPutative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin 0.002530.05099&radic S000000456DUT1YBR252WverifieddUTPase, catalyzes hydrolysis of dUTP to dUMP and PPi, therebypreventing incorporation of uracil into DNAduring replication; critical for themaintenance of genetic stability 0.002520.05099&radic&radic&radic S000002309RPC53YDL150WverifiedRNA polymerase III subunit C53 0.002520.05099&radic&radic&radic&radic S000002458YDR051CUncharacterizedHypothetical protein 0.002520.05099&radic&radic&radic S000005252KRI1YNL308CverifiedEssential nucleolar protein required for 40S ribosomebiogenesis; physically and functionallyinteracts with Krr1p 0.002520.05077&radic S000000851TPA1YER049WuncharacterizedProtein of unknown function; interacts with Sup45p (eRF1),Sup35p (eRF3) and Pab1p; has a role intranslation termination efficiency, mRNApoly(A) tail length and mRNA stability 0.002520.05077&radic&radic&radic S000005036YNL092WUncharacterizedPutative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene 0.002510.05022&radic S000003078CUE3YGL110CuncharacterizedProtein of unknown function; has a CUE domain that bindsubiquitin, which may facilitate intramolecularmonoubiquitination 0.00250.05022&radic&radic S000004097REX3YLR107WverifiedRNA exonuclease; required for maturation of the RNA componentof RNase MRP; functions redundantly with Rnh70pand Rex2p in processing of U5 snRNA and RNase PRNA; member of RNase D family of exonucleases 0.002510.05022&radic&radic S000000464RGD1YBR260CverifiedGTPase-activating protein (RhoGAP) for Rho3p and Rho4p,possibly involved in control of actincytoskeleton organization 0.002510.05022&radic S000001061DED81YHR019CverifiedCytosolic asparaginyl-tRNA synthetase, required for proteinsynthesis, catalyzes the specific attachment ofasparagine to its cognate tRNA 0.002510.05022 S000004199PNP1YLR209CverifiedPurine nucleoside phosphorylase, specifically metabolizesinosine and guanosine nucleosides 0.00250.05011&radic S000004361SSQ1YLR369WverifiedMitochondrial hsp70-type molecular chaperone, required forassembly of iron/sulfur clusters into proteinsat a step after cluster synthesis, and formaturation of Yfh1p, which is a homolog ofhuman frataxin implicated in Friedreich'sataxia 0.00250.05011&radic&radic&radic&radic S000003704SET2YJL168CverifiedHistone methyltransferase with a role in transcriptionalelongation, methylates a lysine residue ofhistone H3; associates with the C-terminaldomain of Rpo21p; histone methylation activityis regulated by phosphorylation status ofRpo21p 0.002480.04932&radic&radic&radic S000005779NAT5YOR253WverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.002480.04932&radic&radic S000000168RPS8AYBL072CverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps8Bp and has similarity to ratS8 ribosomal protein 0.002490.04932&radic S000004520IMD4YML056CverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.002480.04932 S000001622CTK1YKL139WverifiedCatalytic (alpha) subunit of C-terminal domain kinase I(CTDK-I), which phosphorylates the C-terminalrepeated domain of the RNA polymerase II largesubunit (Rpo21p) to affect both transcriptionand pre-mRNA 3' end processing 0.002490.04932&radic&radic S000002990STT3YGL022WverifiedSubunit of the oligosaccharyltransferase complex of the ERlumen, which catalyzes asparagine-linkedglycosylation of newly synthesized proteins;forms a subcomplex with Ost3p and Ost4p and isdirectly involved in catalysis 0.002480.04901 S000003925RTT109YLL002WverifiedProtein with a role in regulation of Ty1 transposition 0.002460.04826&radic&radic&radic&radic S000000631RRP43YCR035CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp4p and Dis3p; required for efficientmaturation of 5.8S, 18S and 25S rRNA 0.002460.04826&radic&radic S000003068SEH1YGL100WverifiedNuclear pore protein that is part of the evolutionarilyconserved Nup84p complex (Nup84p, Nup85p,Nup120p, Nup145p, and Seh1p); homologous toSec13p 0.002460.04826&radic&radic S000001210THP2YHR167WverifiedSubunit of the THO complex, which connects transcriptionelongation and mitotic recombination, and ofthe TREX complex, which is recruited toactivated genes and couples transcription tomRNA export; involved in telomere maintenance 0.002450.04812&radic S000003451YGR219WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C 0.002450.04791&radic&radic&radic&radic&radic S000001007RPS20YHL015WverifiedProtein component of the small (40S) ribosomal subunit;overproduction suppresses mutations affectingRNA polymerase III-dependent transcription; hassimilarity to E. coli S10 and rat S20 ribosomalproteins 0.002440.04757 S000004096MDN1YLR106CverifiedHuge dynein-related AAA-type ATPase (midasin), forms extendedpre-60S particle with the Rix1 complex(Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependentremodeling of 60S subunits and subsequentexport from nucleoplasm to cytoplasm 0.002440.04751&radic&radic&radic S000000865THO1YER063WverifiedProtein of unknown function; overproduction suppresses thetranscriptional defect caused by an hpr1mutation 0.002440.04751&radic&radic S000002697RTT103YDR289CverifiedProtein that interacts with exonuclease Rat1p and Rai1p andplays a role in transcription termination byRNA polymerase II, has an RPR domain(carboxy-terminal domain interacting domain);also involved in regulation of Ty1transposition 0.002430.04709&radic&radic S000005192RPA49YNL248CverifiedRNA polymerase I subunit A49 0.002420.04688&radic S000003623TRL1YJL087CverifiedtRNA ligase, required for tRNA splicing; composed of threeessential domains containing thephosphodiesterase, polynucleotide kinase, andligase activities required for ligation;localized at the inner membrane of the nuclearenvelope 0.002420.04688&radic&radic&radic&radic S000004560RAD10YML095CverifiedSingle-stranded DNA endonuclease (with Rad1p), cleavessingle-stranded DNA during nucleotide excisionrepair and double-strand break repair; subunitof Nucleotide Excision Repair Factor 1 (NEF1);homolog of human ERCC1 protein 0.002420.04644&radic&radic&radic&radic&radic S000005976LGE1YPL055CverifiedProtein of unknown function; null mutant forms abnormally largecells 0.002410.04618&radic S000002890CWC21YDR482CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; may bind RNA; has similarityto S. pombe Cwf21p 0.002410.04618&radic&radic S000005554CIN5YOR028CverifiedBasic leucine zipper transcriptional factor of the yAP-1 familythat mediates pleiotropic drug resistance andsalt tolerance; localizes constitutively to thenucleus 0.00240.04591&radic&radic S000000730YEA4YEL004WverifiedUridine diphosphate-N-acetylglucosamine (UDP-GlcNAc)transporter required for cell wall chitinsynthesis; localized to the ER 0.00240.04588&radic&radic&radic&radic S000000293YBR089WDubiousHypothetical protein 0.002390.04557 S000000680TUP1YCR084CverifiedGeneral repressor of transcription, forms complex with Cyc8p,involved in the establishment of repressivechromatin structure through interactions withhistones H3 and H4, appears to enhanceexpression of some genes 0.00240.04557&radic&radic S000004738RRB1YMR131CverifiedEssential nuclear protein involved in early steps of ribosomebiogenesis; physically interacts with theribosomal protein Rpl3p 0.002390.04482&radic&radic S000002835RPN9YDR427WverifiedNon-ATPase regulatory subunit of the 26S proteasome, hassimilarity to putative proteasomal subunits inother species; null mutant is temperaturesensitive and exhibits cell cycle andproteasome assembly defects 0.002390.04482&radic&radic&radic&radic&radic S000000069BUD14YAR014CverifiedProtein involved in bud-site selection, Bud14p-Glc7p complexfunctions as a cortical regulator of dynein;diploid mutants display a random buddingpattern instead of the wild-type bipolarpattern 0.002390.04482&radic&radic&radic S000001800SRP40YKR092CverifiedNucleolar, serine-rich protein with a role in preribosomeassembly or transport; may function as achaperone of small nucleolar ribonucleoproteinparticles (snoRNPs); immunologically andstructurally to rat Nopp140 0.002380.04482&radic S000000879YER077CUncharacterizedHypothetical protein 0.002380.04482&radic&radic&radic S000003436ADE3YGR204WverifiedCytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase,involved in single carbon metabolism andrequired for biosynthesis of purines,thymidylate, methionine, and histidine 0.002370.04431&radic&radic S000001094CIC1YHR052WverifiedEssential protein that interacts with proteasome components andhas a potential role in proteasome substratespecificity; also copurifies with 66Spre-ribosomal particles 0.002370.04431&radic S000005354YNR071CUncharacterizedHypothetical protein 0.002370.04431&radic&radic S000003504YGR272CUncharacterizedPutative protein of unknown function; deletion mutant has defects in pre-rRNA processing; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 0.002370.04431&radic&radic&radic&radic&radic S000005131YNL187WUncharacterizedPutative protein of unknown function; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; may interact with or be fuctionally redundant with Prp40p 0.002360.04399&radic S000005724BFR1YOR198CverifiedComponent of mRNP complexes associated with polyribosomes;implicated in secretion and nuclearsegregation; multicopy suppressor of BFA(Brefeldin A) sensitivity 0.002350.04378&radic&radic S000004118DCN1YLR128WverifiedPutative Nedd8 ligase; binds Nedd8; involved in cullinneddylation; not essential; similar toC.elegans DCN-1; contains UBA-likeubiquitin-binding domain and a DUF298 domain 0.002350.04348&radic&radic S000003664PBS2YJL128CverifiedMAP kinase kinase that plays a pivotal role in the osmosensingsignal-transduction pathway, activated undersevere osmotic stress 0.002340.04305&radic&radic S000002266KIN28YDL108WverifiedSerine/threonine protein kinase, subunit of the transcriptionfactor TFIIH; involved in transcriptioninitiation at RNA polymerase II promoters 0.002340.04305&radic&radic&radic S000000627RPS14AYCR031CverifiedRibosomal protein 59 of the small subunit, required forribosome assembly and 20S pre-rRNA processing;mutations confer cryptopleurine resistance;nearly identical to Rps14Bp and similar to E.coli S11 and rat S14 ribosomal proteins 0.002340.04305&radic S000002494RRP1YDR087CverifiedEssential evolutionarily conserved nucleolar protein necessaryfor biogenesis of 60S ribosomal subunits andprocessing of pre-rRNAs to mature rRNAs,associated with several distinct 66Spre-ribosomal particles 0.002330.04278&radic&radic&radic&radic&radic S000004101YLR111WDubiousDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data 0.002330.04248 S000005809YOR283WUncharacterizedPutative protein of unknown function with some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene 0.002330.04248&radic&radic&radic S000003772YJR011CUncharacterizedHypothetical protein 0.002320.04228&radic&radic&radic&radic S000002688RRP45YDR280WverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.002320.04228&radic&radic S000000019CCR4YAL021CverifiedComponent of the CCR4-NOT transcriptional complex, which isinvolved in regulation of gene expression;component of the major cytoplasmic deadenylase,which is involved in mRNA poly(A) tailshortening 0.002320.042 S000003737ECM25YJL201WverifiedNon-essential protein of unknown function; promoter contains aconsensus binding sequence for factor Abf1p 0.002310.04179&radic&radic&radic S000003632MRPL49YJL096WverifiedMitochondrial ribosomal protein of the large subunit 0.002310.04179&radic&radic&radic&radic S000003782REC107YJR021CverifiedProtein involved in early stages of meiotic recombination;involved in altering chromatin structure at DNAdouble-stranded break sites and in coordinationbetween the initiation of recombination and thefirst division of meiosis 0.002310.04177&radic&radic&radic S000001102VMA22YHR060WverifiedIntegral membrane protein that is required for vacuolarH+-ATPase (V-ATPase) function, although not anactual component of the V-ATPase complex;functions in the assembly of the V-ATPase;localized to the yeast endoplasmic reticulum(ER) 0.002310.04177&radic&radic S000003446RPS0AYGR214WverifiedProtein component of the small (40S) ribosomal subunit, nearlyidentical to Rps0Bp; required for maturation of18S rRNA along with Rps0Bp; deletion of eitherRPS0 gene reduces growth rate, deletion of bothgenes is lethal 0.002310.04161 S000002559GIR2YDR152WverifiedHighly-acidic cytoplasmic RWD domain-containing protein ofunknown function, sensitive to proteolysis,N-terminal region has high content of acidicamino acid residues, putative IUP(intrinsically unstructured protein) 0.002310.04161&radic&radic S000004871YMR258CUncharacterizedProtein of unknown function with similarity to F-box proteins; physically interacts with Skp1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR258C is not an essential gene 0.002310.04161&radic&radic&radic&radic&radic S000002826RPL12BYDR418WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl12Ap; rpl12a rpl12b doublemutant exhibits slow growth and slowtranslation; has similarity to E. coli L11 andrat L12 ribosomal proteins 0.00230.04099 S000005978SUR1YPL057CverifiedProbable catalytic subunit of a mannosylinositolphosphorylceramide (MIPC) synthase, forms acomplex with probable regulatory subunit Csg2p;function in sphingolipid biosynthesis isoverlapping with that of Csh1p 0.00230.04099 S000005133SRP1YNL189WverifiedKaryopherin alpha homolog, forms a dimer with karyopherin betaKap95p to mediate import of nuclear proteins,binds the nuclear localization signal of thesubstrate during import; may also play a rolein regulation of protein degradation 0.002290.04099&radic S000001492MRT4YKL009WverifiedProtein involved in mRNA turnover and ribosome assembly,localizes to the nucleolus 0.00230.04099&radic&radic&radic S000001803MLP1YKR095WverifiedMyosin-like protein associated with the nuclear envelope,connects the nuclear pore complex with thenuclear interior; involved with Tel1p intelomere length control; involved with Pml1pand Pml39p in nuclear retention of unsplicedmRNAs 0.002290.0402&radic S000003105SEC27YGL137WverifiedEssential beta'-coat protein of the COPI coatomer, involved inER-to-Golgi and Golgi-to-ER transport; containsWD40 domains that mediate cargo selectiveinteractions; 45% sequence identity tomammalian beta'-COP 0.002290.0402&radic&radic&radic S000005034RHO2YNL090WverifiedNon-essential small GTPase of the Rho/Rac subfamily of Ras-likeproteins, involved in the establishment of cellpolarity and in microtubule assembly 0.002280.0402&radic&radic S000003149GTS1YGL181WverifiedProtein containing a zinc-finger in the N-terminus and a longGln-rich region in the C-terminus; regulatesultradian rhythm, cell size, cell cycle,lifespan, sporulation, heat tolerance, andmultidrug transport 0.002280.0402&radic&radic&radic S000002390BRE4YDL231CverifiedZinc finger protein containing five transmembrane domains; nullmutant exhibits strongly fragmented vacuolesand sensitivity to brefeldin A, a drug which isknown to affect intracellular transport 0.002280.0402&radic&radic&radic S000000097ECM15YBL001CverifiedNon-essential protein of unknown function, likely exists astetramer, may be regulated by the binding ofsmall-molecule ligands (possibly sulfate ions),may have a role in yeast cell-wall biogenesis 0.002270.03969&radic&radic S000000252RPS11BYBR048WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps11Ap and has similarity to E.coli S17 and rat S11 ribosomal proteins 0.002270.03969&radic&radic S000005256RFA2YNL312WverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 0.002270.03969&radic&radic S000004952SIS1YNL007CverifiedType II HSP40 co-chaperone that interacts with the HSP70protein Ssa1p; not functionally redundant withYdj1p due to due to substrate specificity;shares similarity with bacterial DnaJ proteins 0.002270.03969&radic S000003336SRB5YGR104CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation; involved intelomere maintenance 0.002260.03934&radic&radic&radic S000003289LST7YGR057CverifiedProtein possibly involved in a post-Golgi secretory pathway;required for the transport ofnitrogen-regulated amino acid permease Gap1pfrom the Golgi to the cell surface 0.002260.03934 S000004216BUR2YLR226WverifiedCyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2pcomprise a CDK-cyclin complex involved intranscriptional regulation through itsphosphorylation of the carboxy-terminal domainof the largest subunit of RNA polymerase II 0.002260.03934&radic&radic S000002761TRR1YDR353WverifiedCytoplasmic thioredoxin reductase, key regulatory enzyme thatdetermines the redox state of the thioredoxinsystem, which acts as a disulfide reductasesystem and protects cells against bothoxidative and reductive stress 0.002260.03927&radic S000000371POP7YBR167CverifiedSubunit of both RNase MRP, which cleaves pre-rRNA, and nuclearRNase P, which cleaves tRNA precursors togenerate mature 5' ends 0.002250.03906&radic&radic&radic S000005291LRO1YNR008WverifiedAcyltransferase that catalyzes diacylglycerol esterification;one of several acyltransferases that contributeto triglyceride synthesis; putative homolog ofhuman lecithin cholesterol acyltransferase 0.002250.03896&radic&radic&radic&radic S000002194PUS9YDL036CverifiedMitochondrial tRNA pseudouridine synthase involved inpseudouridylation of mitochondrial tRNAs atposition 32 0.002250.03872&radic&radic&radic&radic S000000450YBR246WUncharacterizedHypothetical protein 0.002240.03868&radic&radic S000000360SLI15YBR156CverifiedSubunit of the Ipl1p-Sli15p-Bir1p complex that regulateskinetochore-microtubule attachments, activationof the spindle tension checkpoint, and mitoticspindle disassembly; regulates the activity andlocalization of the Ipl1p aurora kinase 0.002240.03825&radic&radic&radic S000000029GIP4YAL031CverifiedCytoplasmic Glc7-interacting protein whose overexpressionrelocalizes Glc7p from the nucleus and preventschromosome segregation; potential Cdc28psubstrate 0.002240.03825&radic S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.002230.03787&radic&radic S000002487VPS41YDR080WverifiedVacuolar membrane protein that is a subunit of the homotypicvacuole fusion and vacuole protein sorting(HOPS) complex; essential for membrane dockingand fusion at the Golgi-to-endosome andendosome-to-vacuole stages of protein transport 0.002230.03787&radic&radic S000005356YNR073CUncharacterizedHypothetical protein 0.002230.03787&radic&radic S000006043TFB2YPL122CverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, involved in transcriptioninitiation, required for nucleotide excisionrepair, similar to 52 kDa subunit of humanTFIIH 0.002230.03787&radic&radic&radic S000000668RSA4YCR072CverifiedWD-repeat protein involved in ribosome biogenesis; may interactwith ribosomes; required for maturation andefficient intra-nuclear transport or pre-60Sribosomal subunits, localizes to the nucleolus 0.002220.03767&radic&radic S000001246RPS4BYHR203CverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps4Ap and has similarity to ratS4 ribosomal protein 0.002210.03712 S000004094YLR104WUncharacterizedHypothetical protein 0.002210.03712 S000001655EBP2YKL172WverifiedEssential protein required for the maturation of 25S rRNA and60S ribosomal subunit assembly, localizes tothe nucleolus; constituent of 66S pre-ribosomalparticles 0.002210.0371&radic&radic&radic S000003081SLD3YGL113WverifiedProtein involved in the initiation of DNA replication, requiredfor proper assembly of replication proteins atthe origins of replication; interacts withCdc45p 0.002190.03658&radic&radic&radic&radic S000005553STI1YOR027WverifiedHsp90 cochaperone, interacts with the Ssa group of thecytosolic Hsp70 chaperones; activates theATPase activity of Ssa1p; homolog of mammalianHop protein 0.002190.03658&radic S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 0.00220.03658&radic&radic&radic S000004040YLR050CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene 0.002190.03658&radic&radic S000000467SHM1YBR263WverifiedMitochondrial serine hydroxymethyltransferase, involved inone-carbon metabolism 0.002190.03634 S000005787RPN8YOR261CverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome;has similarity to the human p40 proteasomalsubunit and to another S. cerevisiae regulatorysubunit, Rpn11p 0.002190.03634&radic&radic S000002780VPS74YDR372CverifiedNon-essential protein of unknown function involved in vacuolarprotein sorting; belongs to a family ofcytosolic Golgi-associated proteins suggestingthat it may play a role in secretion; alsodetected in the nucleus 0.002190.03634&radic&radic&radic S000003136HUR1YGL168WuncharacterizedProtein required for hydroxyurea resistance; has possible rolesin DNA replication and maintenance of propertelomere length 0.002190.03634&radic&radic&radic&radic S000004754TIF34YMR146CverifiedSubunit of the core complex of translation initiation factor3(eIF3), which is essential for translation 0.002180.0362 S000002549PEX7YDR142CverifiedPeroxisomal signal receptor for the N-terminal nonapeptidesignal (PTS2) of peroxisomal matrix proteins;WD repeat protein; defects in human homologcause lethal rhizomelic chondrodysplasiapunctata (RCDP) 0.002180.0362 S000001305CBR1YIL043CverifiedMicrosomal cytochrome b reductase, not essential for viability;also detected in mitochondria; mutation inconserved NADH binding domain of the humanortholog results in type I methemoglobinemia 0.002180.036&radic&radic&radic&radic S000003308MRPL25YGR076CverifiedMitochondrial ribosomal protein of the large subunit 0.002180.036&radic&radic&radic S000001308MET30YIL046WverifiedF-box protein containing five copies of the WD40 motif,controls cell cycle function, sulfurmetabolism, and methionine biosynthesis as partof the ubiquitin ligase complex; interacts withand regulates Met4p, localizes within thenucleus 0.002180.036&radic&radic&radic&radic S000000124YBL028CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis 0.002180.036&radic&radic&radic&radic S000002858RPS18AYDR450WverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps18Bp and has similarity to E.coli S13 and rat S18 ribosomal proteins 0.002180.036 S000004005BRE2YLR015WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres;involved in telomere maintenance; similar totrithorax-group protein ASH2L 0.002180.03591&radic&radic&radic&radic&radic S000005603RTS2YOR077WverifiedBasic zinc-finger protein, similar to human and mouse Kin17proteins which are chromatin-associatedproteins involved in UV response and DNAreplication 0.002170.03582&radic S000005197TEX1YNL253WverifiedProtein involved in mRNA export, component of the transcriptionexport (TREX) complex 0.002170.03529 S000005475PAP2YOL115WverifiedCatalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), anuclear poly (A) polymerase complex involved inRNA quality control; catalyzes polyadenylationof unmodified tRNAs, and snoRNA and rRNAprecursors; disputed role as a DNA polymerase