Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0043630 "ncRNA polyadenylation during polyadenylation-dependent ncRNA catabolism"

GO branch: BP
Number of currently annotated genes: 5

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest Ho:S000002334Ho:S000001341PF03828IPR002058Giaever:fenpropimorph_4.69uMTAP:205Harbison:MSS11_BUT14Giaever:5-fluorouracil_9.6uMPF00098IPR012961 S000005475PAP2YOL115WverifiedCatalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), anuclear poly (A) polymerase complex involved inRNA quality control; catalyzes polyadenylationof unmodified tRNAs, and snoRNA and rRNAprecursors; disputed role as a DNA polymerase &radic0.248690.97297&radic&radic S000005243TRF5YNL299WverifiedPoly (A) polymerase involved in nuclear RNA quality controlbased on: homology with Trf4p, geneticinteractions with TRF4 mutants, physicalinteraction with Mtr4p (TRAMP subunit), and bydirect assay; disputed role as a sigma DNApolymerase &radic0.166840.89119&radic&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p &radic0.071780.67175&radic&radic&radic&radic S000002334AIR2YDL175CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p; may regulatemethylation of Npl3p, which modulates Npl3pfunction in mRNA processing and export; hassimilarity to Air1p &radic0.051930.60913&radic&radic S000006111NAB3YPL190CverifiedSingle stranded DNA binding protein; acidic ribonucleoprotein;required for termination of non-poly(A)transcripts and efficient splicing; interactswith Nrd1p 0.028340.51062&radic S000003163GCN1YGL195WverifiedPositive regulator of the Gcn2p kinase activity, forms acomplex with Gcn20p; proposed to stimulateGcn2p activation by an uncharged tRNA 0.014690.38267&radic S000001710PAP1YKR002WverifiedPoly(A) polymerase, one of three factors required for mRNA3'-end polyadenylation, forms multiproteincomplex with polyadenylation factor I (PF I),also required for mRNA nuclear export; may alsopolyadenylate rRNAs 0.006420.27028 S000004390SKI2YLR398CverifiedPutative RNA helicase, involved in exosome mediated 3' to 5'mRNA degradation and translation inhibition ofnon-poly(A) mRNAs; forms complex with Ski3p andSki8p; required for repressing propagation ofdsRNA viruses 0.005430.23639&radic S000002495SLU7YDR088CverifiedRNA splicing factor, required for ATP-independent portion of2nd catalytic step of spliceosomal RNAsplicing; interacts with Prp18p; contains zincknuckle domain 0.004850.21557&radic S000004106MSL5YLR116WverifiedComponent of the commitment complex, which defines the firststep in the splicing pathway; essential proteinthat interacts with Mud2p and Prp40p, forming abridge between the intron ends; also involvedin nuclear retention of pre-mRNA 0.00430.19238&radic S000005199GIS2YNL255CverifiedProtein with seven cysteine-rich CCHC zinc-finger motifs,similar to human CNBP, proposed to be involvedin the RAS/cAMP signaling pathway 0.00410.18128&radic S000000939YER137CUncharacterizedHypothetical protein 0.002760.13036 S000006311YTH1YPR107CverifiedEssential RNA-binding component of cleavage and polyadenylationfactor, contains five zinc fingers; requiredfor pre-mRNA 3'-end processing andpolyadenylation 0.00260.12266 S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif 0.002480.11879 S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.001390.0706 S000003506TAF1YGR274CverifiedTFIID subunit (145 kDa), involved in RNA polymerase IItranscription initiation, has histoneacetyltransferase activity, involved inpromoter binding and G1/S progression 0.001320.06609&radic S000005366TOP1YOL006CverifiedTopoisomerase I, nuclear enzyme that relieves torsional strainin DNA by cleaving and re-sealing thephosphodiester backbone; relaxes bothpositively and negatively supercoiled DNA;functions in replication, transcription, andrecombination 0.001220.05959 S000003586MTR4YJL050WverifiedDead-box family ATP dependent helicase required for mRNA exportfrom the nucleus; co-factor of the exosomecomplex, required for 3' end formation of 5.8SrRNA &radic0.001110.05371&radic&radic&radic&radic&radic S000004577ZDS2YML109WverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; paralogof Zds1p 0.000710.03329 S000006379DPB2YPR175WverifiedSecond largest subunit of DNA polymerase II (DNA polymeraseepsilon), required for normal yeast chromosomalreplication; expression peaks at the G1/S phaseboundary; potential Cdc28p substrate 0.000660.03124 S000001111RRP4YHR069CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp43p and Dis3p 0.000640.03066 S000004886ZDS1YMR273CverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; has arole in localization of Bcy1p, a regulatorysubunit of protein kinase A; implicated in mRNAnuclear export 0.000580.02725 S000004284GSP1YLR293CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; regulated by Prp20p,Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p andKap95p; yeast Gsp2p homolog 0.000560.02659 S000005206POL2YNL262WverifiedCatalytic subunit of DNA polymerase epsilon, one of the majorchromosomal DNA replication polymerasescharacterized by processivity and proofreadingexonuclease activity; also involved in DNAsynthesis during DNA repair 0.000540.02579 S000001377NUP159YIL115CverifiedSubunit of the nuclear pore complex that is found exclusivelyon the cytoplasmic side, forms a subcomplexwith Nup82p and Nsp1p, required for mRNA export 0.000480.02184 S000002789YRA1YDR381WverifiedNuclear protein that binds to RNA and to Mex67p, required forexport of poly(A)+ mRNA from the nucleus;member of the REF (RNA and export factorbinding proteins) family; another familymember, Yra2p, can substitute for Yra1pfunction 0.000480.02147 S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.000450.01935&radic&radic S000004520IMD4YML056CverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.00040.01709 S000005480RPL18AYOL120CverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl18Bp and has similarity to ratL18 ribosomal protein; intron of RPL18Apre-mRNA forms stem-loop structures that are atarget for Rnt1p cleavage leading todegradation 0.000370.0138 S000000770IES6YEL044WverifiedProtein that associates with the INO80 chromatin remodelingcomplex under low-salt conditions 0.000360.01319 S000002160NHP10YDL002CverifiedProtein related to mammalian high mobility group proteins;likely component of the INO80 complex, which isan ATP-dependent chromatin-remodeling complex 0.000360.01319 S000000301VPS15YBR097WverifiedMyristoylated serine/threonine protein kinase involved invacuolar protein sorting; functions as amembrane-associated complex with Vps34p; activeform recruits Vps34p to the Golgi membrane;interacts with the GDP-bound form of Gpa1p 0.000360.01308 S000000913SWI4YER111CverifiedDNA binding component of the SBF complex (Swi4p-Swi6p), atranscriptional activator that in concert withMBF (Mbp1-Swi6p) regulates late G1-specifictranscription of targets including cyclins andgenes required for DNA synthesis and repair 0.000350.01235 S000005356YNR073CUncharacterizedHypothetical protein 0.000350.01235 S000003397MRPS35YGR165WverifiedMitochondrial ribosomal protein of the small subunit 0.000340.0122 S000005169CNM67YNL225CverifiedComponent of the spindle pole body outer plaque; required forspindle orientation and mitotic nuclearmigration 0.000340.012 S000000673PAT1YCR077CverifiedTopoisomerase II-associated deadenylation-dependentmRNA-decapping factor; also required forfaithful chromosome transmission, maintenanceof rDNA locus stability, and protection of mRNA3'-UTRs from trimming; functionally linked toPab1p 0.000340.012 S000005194RAD50YNL250WverifiedSubunit of MRX complex, with Mre11p and Xrs2p, involved inprocessing double-strand DNA breaks invegetative cells, initiation of meiotic DSBs,telomere maintenance, and nonhomologous endjoining 0.000340.01191 S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.000340.01173 S000004949HRB1YNL004WverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toGbp2p and Npl3p 0.000330.01155 S000004172SWI6YLR182WverifiedTranscription cofactor, forms complexes with DNA-bindingproteins Swi4p and Mbp1p to regulatetranscription at the G1/S transition; involvedin meiotic gene expression; localizationregulated by phosphorylation; potential Cdc28psubstrate 0.000330.01155 S000001790NUP133YKR082WverifiedSubunit of the Nup84p subcomplex of the nuclear pore complex(NPC), localizes to both sides of the NPC,required to establish a normalnucleocytoplasmic concentration gradient of theGTPase Gsp1p 0.000330.01143&radic S000001123LRP1YHR081WverifiedSubstrate-specific nuclear cofactor for exosome activity in theprocessing of stable RNAs; required fortelomere length maintenance; homolog ofmammalian nuclear matrix protein C1D involvedin regulation of DNA repair and recombination 0.000330.01143&radic S000002430SES1YDR023WverifiedCytosolic seryl-tRNA synthetase, class II aminoacyl-tRNAsynthetase that aminoacylates tRNA(Ser),displays tRNA-dependent amino acid recognitionwhich enhances discrimination of the serinesubstrate, interacts with peroxin Pex21p 0.000330.01128 S000005056DBP2YNL112WverifiedEssential ATP-dependent RNA helicase of the DEAD-box proteinfamily, involved in nonsense-mediated mRNAdecay and rRNA processing 0.000320.01084 S000004682VPS20YMR077CverifiedMyristoylated subunit of ESCRTIII, the endosomal sortingcomplex required for transport of transmembraneproteins into the multivesicular body pathwayto the lysosomal/vacuolar lumen; cytoplasmicprotein recruited to endosomal membranes 0.000330.01084 S000005552BUB3YOR026WverifiedKinetochore checkpoint WD40 repeat protein that localizes tokinetochores during prophase and metaphase,delays anaphase in the presence of unattachedkinetochores; forms complexes with Mad1p-Bub1pand with Cdc20p, binds Mad2p and Mad3p 0.000320.01084 S000002536SAC6YDR129CverifiedFimbrin, actin-bundling protein; cooperates with Scp1p(calponin/transgelin) in the organization andmaintenance of the actin cytoskeleton 0.000320.01084 S000005051YAF9YNL107WverifiedSubunit of both the NuA4 histone H4 acetyltransferase complexand the SWR1 complex, may function toantagonize silencing near telomeres; interactsdirectly with Swc4p, has homology to humanleukemogenic protein AF9, contains a YEATSdomain 0.000320.01084 S000006395QCR2YPR191WverifiedSubunit 2 of the ubiquinol cytochrome-c reductase complex,which is a component of the mitochondrial innermembrane electron transport chain;transcription is regulated by Hap1p,Hap2p/Hap3p, and heme 0.000330.01084 S000005738STE4YOR212WverifiedG protein beta subunit, forms a dimer with Ste18p to activatethe mating signaling pathway, forms aheterotrimer with Gpa1p and Ste18p to dampensignaling; may recruit Rho1p to the polarizedgrowth site during mating; contains WD40repeats 0.000320.01041 S000000968DNF1YER166WverifiedAminophospholipid translocase (flippase) that localizesprimarily to the plasma membrane; contributesto endocytosis, protein transport and cellpolarity; type 4 P-type ATPase 0.000310.01013 S000004252YPT6YLR262CverifiedGTPase, Ras-like GTP binding protein involved in the secretorypathway, required for fusion ofendosome-derived vesicles with the late Golgi,maturation of the vacuolar carboxypeptidase Y;has similarity to the human GTPase, Rab6 0.000320.01013 S000002419RPL4BYDR012WverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl4Ap and has similarity to E.coli L4 and rat L4 ribosomal proteins 0.000310.00983 S000000602RVS161YCR009CverifiedAmphiphysin-like lipid raft protein; subunit of a complex(Rvs161p-Rvs167p) that regulates polarizationof the actin cytoskeleton, endocytosis, cellpolarity, cell fusion and viability followingstarvation or osmotic stress 0.000310.00983 S000004362ARC18YLR370CverifiedSubunit of the ARP2/3 complex, which is required for themotility and integrity of cortical actinpatches 0.00030.00917 S000006177CLN2YPL256CverifiedG1 cyclin involved in regulation of the cell cycle; activatesCdc28p kinase to promote the G1 to S phasetransition; late G1 specific expression dependson transcription factor complexes, MBF(Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) 0.00030.00916 S000002742SWR1YDR334WverifiedSwi2/Snf2-related ATPase that is the structural component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.00030.00916&radic S000001073RRM3YHR031CverifiedDNA helicase involved in rDNA replication and Ty1transposition; relieves replication fork pausesat telomeric regions; structurally andfunctionally related to Pif1p 0.00030.00876 S000004424IMD3YLR432WverifiedInosine monophosphate dehydrogenase, catalyzes the first stepof GMP biosynthesis, member of a four-genefamily in S. cerevisiae, constitutivelyexpressed 0.00030.00851 S000000322TEF2YBR118WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF1; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.00030.00851 S000005606DIA2YOR080WverifiedOrigin-binding F-box protein that forms an SCF ubiquitin ligasecomplex with Skp1p and Cdc53p; plays a role inDNA replication, involved in invasive andpseudohyphal growth 0.00030.00843&radic S000000286UBC4YBR082CverifiedUbiquitin-conjugating enzyme that mediates degradation ofshort-lived and abnormal proteins; interactswith E3-CaM in ubiquitinating calmodulin;interacts with many SCF ubiquitin proteinligases; component of the cellular stressresponse 0.000290.00822 S000003746PEX2YJL210WverifiedRING-finger peroxin, peroxisomal membrane protein with aC-terminal zinc-binding RING domain, formsputative translocation subcomplex with Pex10pand Pex12p which functions in peroxisomalmatrix protein import 0.000290.00789 S000004597MSC1YML128CverifiedProtein of unknown function; mutant is defective in directingmeiotic recombination events to homologouschromatids; the authentic, non-tagged proteinis detected in highly purified mitochondria inhigh-throughput studies 0.000290.00789 S000002777XRS2YDR369CverifiedProtein required for DNA repair; component of the Mre11complex, which is involved in double strandbreaks, meiotic recombination, telomeremaintenance, and checkpoint signaling 0.000290.00789&radic S000004138PEP3YLR148WverifiedVacuolar peripheral membrane protein that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, required for vacuolarbiogenesis, forms complex with Pep5p thatmediates protein transport to the vacuole 0.000280.00702&radic S000004918ADH2YMR303CverifiedGlucose-repressible alcohol dehydrogenase II, catalyzes theconversion of ethanol to acetaldehyde; involvedin the production of certain carboxylateesters; regulated by ADR1 0.000270.00679 S000001789RPF2YKR081CverifiedEssential protein involved in the processing of pre-rRNA andthe assembly of the 60S ribosomal subunit;interacts with ribosomal protein L11; localizespredominantly to the nucleolus; constituent of66S pre-ribosomal particles 0.000270.00653 S000005820RRS1YOR294WverifiedEssential protein that binds ribosomal protein L11 and isrequired for nuclear export of the 60Spre-ribosomal subunit during ribosomebiogenesis; mouse homolog shows alteredexpression in Huntington's disease model mice 0.000270.00653 S000000238HMT1YBR034CverifiedNuclear SAM-dependent mono- and asymmetric argininedimethylating methyltransferase that modifieshnRNPs, including Npl3p and Hrp1p, thusfacilitating nuclear export of these proteins;required for viability of npl3 mutants 0.000270.00653 S000005730DED1YOR204WverifiedATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, requiredfor translation initiation of all yeast mRNAs;mutations in human DEAD-box DBY are a frequentcause of male infertility 0.000230.00392 S000005195NRD1YNL251CverifiedRNA-binding protein that interacts with the C-terminal domainof the RNA polymerase II large subunit(Rpo21p), required for transcriptiontermination and 3' end maturation ofnonpolyadenylated RNAs 0.000210.00302 S000005923SNF8YPL002CverifiedComponent of the ESCRT-II complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; appears to be functionallyrelated to SNF7; involved in glucosederepression 0.00020.00278 S000002545HPR1YDR138WverifiedSubunit of THO/TREX complexes that couple transcriptionelongation with mitotic recombination and withmRNA metabolism and export, subunit of an RNAPol II complex; regulates lifespan; involved intelomere maintenance; similar to Top1p 0.00020.00278 S000004093CDC45YLR103CverifiedDNA replication initiation factor; recruited to MCM pre-RCcomplexes at replication origins; promotesrelease of MCM from Mcm10p, recruits elongationmachinery; mutants in human homolog may causevelocardiofacial and DiGeorge syndromes 0.00020.00278 S000000120NCL1YBL024WverifiedS-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase,methylates cytosine to m5C at several positionsin tRNAs and intron-containing pre-tRNAs;similar to Nop2p and human proliferationassociated nucleolar protein p120 0.00020.00271 S000004075ARP6YLR085CverifiedActin-related protein that binds nucleosomes; a component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.00020.00271 S000003288RSC1YGR056WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; containstwo essential bromodomains, a bromo-adjacenthomology (BAH) domain, and an AT hook 0.000190.00261 S000004181PEX13YLR191WverifiedIntegral peroxisomal membrane receptor for the PTS1 peroxisomalmatrix protein signal recognition factor Pex5p,required for the translocation of peroxisomalmatrix proteins, also interacts with Pex7p andPex14p, contains a C-terminal SH3 domain 0.000190.00257 S000004415ATG17YLR423CverifiedProtein that interacts with and is required for activation ofApg1p protein kinase; involved in autophagy butnot in the Cvt (cytoplasm to vacuole targeting)pathway 0.000190.00253 S000003626DPB11YJL090CverifiedEssential BRCT repeat protein, required on the prereplicativecomplex at replication origins for loading DNApolymerases to initiate DNA synthesis, alsorequired for S/M checkpoint control 0.000190.00251 S000006005BRO1YPL084WverifiedCytoplasmic class E vacuolar protein sorting (VPS) factor thatcoordinates deubiquitination in themultivesicular body (MVB) pathway by recruitingDoa4p to endosomes 0.000190.00242 S000002688RRP45YDR280WverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.000190.00242 S000004984BDP1YNL039WverifiedEssential subunit of RNA polymerase III transcription factor(TFIIIB), which is involved in transcription ofgenes encoding tRNAs, 5S rRNA, U6 snRNA, andother small RNAs 0.000180.00241 S000000631RRP43YCR035CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp4p and Dis3p; required for efficientmaturation of 5.8S, 18S and 25S rRNA 0.000180.00241 S000002360TRM8YDL201WverifiedSubunit of a tRNA methyltransferase complex composed of Trm8pand Trm82p that catalyzes 7-methylguanosinemodification of tRNA 0.000180.00235 S000004903HAS1YMR290CverifiedATP-dependent RNA helicase; localizes to both the nuclearperiphery and nucleolus; highly enriched innuclear pore complex fractions; constituent of66S pre-ribosomal particles 0.000180.00233 S000003797HUL4YJR036CverifiedProtein with similarity to hect domain E3 ubiquitin-proteinligases, not essential for viability 0.000180.00231 S000000039CDC24YAL041WverifiedGuanine nucleotide exchange factor (GEF or GDP-release factor)for Cdc42p; required for polarity establishmentand maintenance, and mutants have morphologicaldefects in bud formation and shmooing 0.000180.00226&radic S000000967PAB1YER165WverifiedPoly(A) binding protein, part of the 3'-end RNA-processingcomplex, mediates interactions between the 5'cap structure and the 3' mRNA poly(A) tail,involved in control of poly(A) tail length,interacts with translation factor eIF-4G 0.000170.00224 S000004029RIC1YLR039CverifiedProtein involved in retrograde transport to the cis-Golginetwork; forms heterodimer with Rgp1p that actsas a GTP exchange factor for Ypt6p; involved intranscription of rRNA and ribosomal proteingenes 0.000170.00224 S000006067NOP53YPL146CverifiedNucleolar protein; involved in biogenesis of the 60S subunit ofthe ribosome; interacts with rRNA processingfactors Cbf5p and Nop2p; null mutant is viablebut growth is severely impaired 0.000170.0022 S000003054MAD1YGL086WverifiedCoiled-coil protein involved in the spindle-assemblycheckpoint; phosphorylated by Mps1p uponcheckpoint activation which leads to inhibitionof the activity of the anaphase promotingcomplex; forms a complex with Mad2p 0.000170.0022 S000004842RRP5YMR229CverifiedProtein required for the synthesis of both 18S and 5.8S rRNA;C-terminal region is crucial for the formationof 18S rRNA and N-terminal region is requiredfor the 5.8S rRNA; component of small ribosomalsubunit (SSU) processosome 0.000170.0022 S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 0.000170.00217 S000005143GCR2YNL199CverifiedTranscriptional activator of genes involved in glycolysis;interacts and functions with the DNA-bindingprotein Gcr1p 0.000170.00213 S000002847LRS4YDR439WverifiedProtein involved in rDNA silencing; positively chargedcoiled-coil protein with limited similarity tomyosin 0.000170.00213 S000001508PAN3YKL025CverifiedEssential subunit of the Pan2p-Pan3p poly(A)-ribonucleasecomplex, which acts to control poly(A) taillength and regulate the stoichiometry andactivity of postreplication repair complexes 0.000160.00212&radic S000003088PRP43YGL120CverifiedRNA helicase in the DEAH-box family, functions in both RNApolymerase I and polymerase II transcriptmetabolism, involved in release of thelariat-intron from the spliceosome 0.000160.00209 S000002544RGP1YDR137WverifiedSubunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) thatcatalyzes nucleotide exchange on Ypt6p 0.000160.00202 S000003063VPS45YGL095CverifiedProtein of the Sec1p/Munc-18 family, essential for vacuolarprotein sorting; required for the function ofPep12p and the early endosome/late Golgi SNARETlg2p; essential for fusion of Golgi-derivedvesicles with the prevacuolar compartment 0.000160.00202 S000003719MNN11YJL183WverifiedSubunit of a Golgi mannosyltransferase complex that alsocontains Anp1p, Mnn9p, Mnn10p, and Hoc1p, andmediates elongation of the polysaccharidemannan backbone; has homology to Mnn10p 0.000160.00202 S000004745PSO2YMR137CverifiedRequired for a post-incision step in the repair of DNA singleand double-strand breaks that result frominterstrand crosslinks produced by a variety ofmono- and bi-functional psoralen derivatives;induced by UV-irradiation 0.000160.00202&radic S000004467ERG6YML008CverifiedDelta(24)-sterol C-methyltransferase, converts zymosterol tofecosterol in the ergosterol biosyntheticpathway by methylating position C-24; localizedto both lipid particles and mitochondrial outermembrane 0.000160.00202 S000000818BIM1YER016WverifiedMicrotubule-binding protein that together with Kar9p makes upthe cortical microtubule capture site anddelays the exit from mitosis when the spindleis oriented abnormally 0.000150.00197 S000004844PEP5YMR231WverifiedPeripheral vacuolar membrane protein required for proteintrafficking and vacuole biogenesis; formscomplex with Pep3p that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, also interacts with Pep7p 0.000150.00197 S000005133SRP1YNL189WverifiedKaryopherin alpha homolog, forms a dimer with karyopherin betaKap95p to mediate import of nuclear proteins,binds the nuclear localization signal of thesubstrate during import; may also play a rolein regulation of protein degradation 0.000140.00189 S000002905ITR1YDR497CverifiedMyo-inositol transporter with strong similarity to the minormyo-inositol transporter Itr2p, member of thesugar transporter superfamily; expression isrepressed by inositol and choline via Opi1p andderepressed via Ino2p and Ino4p 0.000140.00184 S000002767VID21YDR359CverifiedComponent of the NuA4 histone acetyltransferase complex 0.000130.00182 S000004329VRP1YLR337CverifiedProline-rich actin-associated protein involved in cytoskeletalorganization and cytokinesis; related tomammalian Wiskott-Aldrich syndrome protein(WASP)-interacting protein (WIP) 0.000130.00178 S000001129YHR087WVerifiedProtein involved in RNA metabolism, one of two yeast homologs (with Sdo1p/Ylr022cp) of the human protein SBDS responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome, also conserved in Archaea 0.000130.00176 S000003121PEX14YGL153WverifiedPeroxisomal membrane protein that is a central component of theperoxisomal protein import machinery, interactswith PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomalmatrix protein signal recognition factors andmembrane receptor Pex13p 0.000130.00176 S000005187SLA2YNL243WverifiedTransmembrane actin-binding protein involved in membranecytoskeleton assembly and cell polarization;adaptor protein that links actin to clathrinand endocytosis; present in the actin corticalpatch of the emerging bud tip; dimer in vivo 0.000130.00175 S000004409VPS36YLR417WverifiedComponent of the ESCRT-II complex; contains the GLUE (GRAM LikeUbiquitin binding in EAP45) domain which isinvolved in interactions with ESCRT-I andubiquitin-dependent sorting of proteins intothe endosome 0.000130.00174&radic S000004349RSC2YLR357WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; involvedin telomere maintenance 0.000130.00174 S000006305SNT309YPR101WverifiedComponent of NineTeen complex (NTC) containing Prp19p involvedin mRNA splicing, interacts physically andgenetically with Prp19p 0.000120.00173 S000001927SMC2YFR031CverifiedComponent of the condensin complex, essential SMC chromosomalATPase family member that forms a complex withSmc4p to form the active ATPase; Smc2p/Smc4pcomplex binds DNA, possibly in the cleft formedby the coiled-coil of the folded dimer 0.000120.00173 S000005028END3YNL084CverifiedEH domain-containing protein involved in endocytosis, actincytoskeletal organization and cell wallmorphogenesis; forms a complex with Sla1p andPan1p 0.000120.00172&radic S000000682CSM1YCR086WverifiedNucleolar protein that forms a complex with Lrs4p which bindsMam1p at kinetochores during meiosis I tomediate accurate chromosome segregation, may beinvolved in premeiotic DNA replication;possible role in telomere maintenance 0.000120.00172&radic S000000435SWC5YBR231CuncharacterizedProtein of unknown function, component of the SWR1 complex,which exchanges histone variant H2AZ (Htz1p)for chromatin-bound histone H2A 0.000120.00172 S000004187SIK1YLR197WverifiedEssential evolutionarily-conserved nucleolar protein componentof the box C/D snoRNP complexes that direct2'-O-methylation of pre-rRNA during itsmaturation; overexpression causes spindleorientation defects 0.000120.00171 S000004363ROM2YLR371WverifiedGDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutationsare synthetically lethal with mutations inrom1, which also encodes a GEP 0.000120.00171 S000005667ARP8YOR141CverifiedNuclear actin-related protein involved in chromatin remodeling,component of chromatin-remodeling enzymecomplexes 0.000120.00171 S000000122LSM2YBL026WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.000120.0017&radic S000001596RAD27YKL113Cverified5' to 3' exonuclease, 5' flap endonuclease, required forOkazaki fragment processing and maturation aswell as for long-patch base-excision repair;member of the S. pombe RAD2/FEN1 family 0.000120.0017 S000003863VPS25YJR102CverifiedComponent of the ESCRT-II complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome 0.000120.00169 S000005831ISW2YOR304WverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPasecomponent that, with Itc1p, forms a complexrequired for repression of a-specific genes,INO1, and early meiotic genes during mitoticgrowth 0.000120.00169&radic S000004683CTF18YMR078CverifiedSubunit of a complex with Ctf8p that shares some subunits withReplication Factor C and is required for sisterchromatid cohesion; may have overlappingfunctions with Rad24p in the DNA damagereplication checkpoint 0.000120.00169 S000003818CDC8YJR057WverifiedThymidylate and uridylate kinase, functions in de novobiosynthesis of pyrimidinedeoxyribonucleotides; converts dTMP to dTDP anddUMP to dUTP; essential for mitotic and meioticDNA replication; homologous to S. pombe Tmp1p 0.000120.00169 S000003336SRB5YGR104CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation; involved intelomere maintenance 0.000120.00167 S000002555KGD2YDR148CverifiedDihydrolipoyl transsuccinylase, a component of themitochondrial alpha-ketoglutarate dehydrogenasecomplex, which catalyzes a step in thetricarboxylic acid (TCA) cycle, the oxidativedecarboxylation of alpha-ketoglutarate tosuccinyl-CoA 0.000120.00167 S000001756NAP1YKR048CverifiedProtein that interacts with mitotic cyclin Clb2p; required forthe regulation of microtubule dynamics duringmitosis; controls bud morphogenesis; involvedin the transport of H2A and H2B histones to thenucleus 0.000120.00167 S000005372HTZ1YOL012CverifiedHistone variant H2AZ, exchanged for histone H2A in nucleosomesby the SWR1 complex; involved intranscriptional regulation through preventionof the spread of silent heterochromatin 0.000120.00167 S000003660LSM1YJL124CverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)involved in degradation of cytoplasmic mRNAs 0.000120.00167 S000004732STO1YMR125WverifiedLarge subunit of the nuclear mRNA cap-binding protein complex,interacts with Npl3p to carry nuclear poly(A)+mRNA to cytoplasm; also involved in nuclearmRNA degradation and telomere maintenance;orthologous to mammalian CBP80 0.000120.00167 S000003062PAN2YGL094CverifiedEssential subunit of the Pan2p-Pan3p poly(A)-ribonucleasecomplex, which acts to control poly(A) taillength and regulate the stoichiometry andactivity of postreplication repair complexes 0.000120.00166 S000004045SPT8YLR055CverifiedSubunit of the SAGA transcriptional regulatory complex but notpresent in SAGA-like complex SLIK/SALSA,required for SAGA-mediated inhibition at somepromoters 0.000120.00166 S000004920UBP15YMR304WverifiedUbiquitin-specific protease that may play a role in ubiquitinprecursor processing 0.000120.00166 S000003095SOH1YGL127CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; involved in telomeremaintenance; conserved with other metazoanMED31 subunits 0.000120.00166 S000004470SPT5YML010WverifiedProtein that forms a complex with Spt4p and mediates bothactivation and inhibition of transcriptionelongation; Spt4p-Spt5p complex also plays arole in pre-mRNA processing 0.000120.00166 S000001886SMC1YFL008WverifiedSubunit of the multiprotein cohesin complex, essential proteininvolved in chromosome segregation and indouble-strand DNA break repair; SMC chromosomalATPase family member, binds DNA with apreference for DNA with secondary structure 0.000120.00166 S000000455MRPS5YBR251WverifiedMitochondrial ribosomal protein of the small subunit 0.000120.00166 S000003406CBP4YGR174CverifiedMitochondrial protein required for assembly of ubiquinolcytochrome-c reductase complex (cytochrome bc1complex); interacts with Cbp3p and function ispartially redundant with that of Cbp3p 0.000110.00163 S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 0.000110.00161 S000001387KGD1YIL125WverifiedComponent of the mitochondrial alpha-ketoglutaratedehydrogenase complex, which catalyzes a keystep in the tricarboxylic acid (TCA) cycle, theoxidative decarboxylation ofalpha-ketoglutarate to form succinyl-CoA 0.000110.00159 S000002467MAK21YDR060WverifiedConstituent of 66S pre-ribosomal particles, required for large(60S) ribosomal subunit biogenesis; involved innuclear export of pre-ribosomes; required formaintenance of dsRNA virus; homolog of humanCAATT-binding protein 0.000110.00159 S000001238NVJ1YHR195WverifiedNuclear envelope protein that interacts with the vacuolarmembrane protein Vac8p to promote formation ofnucleus-vacuole junctions during piecemealmicroautophagy of the nucleus (PMN) 0.000110.00159&radic S000006141RPL1AYPL220WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl1Bpand has similarity to E. coli L1 and rat L10aribosomal proteins; rpl1a rpl1b double nullmutation is lethal 0.000110.00157 S000004925PSE1YMR308CverifiedKaryopherin/importin that interacts with the nuclear porecomplex; acts as the nuclear import receptorfor specific proteins, including Pdr1p, Yap1p,Ste12p, and Aft1p 0.000110.00157 S000006082BEM4YPL161CverifiedProtein involved in establishment of cell polarity and budemergence; interacts with the Rho1p smallGTP-binding protein and with the Rho-typeGTPase Cdc42p; involved in maintenance ofproper telomere length 0.00010.00154 S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus 0.00010.00154&radic S000002718SUM1YDR310CverifiedTranscriptional repressor required for mitotic repression ofmiddle sporulation-specific genes; involved intelomere maintenance, regulated by thepachytene checkpoint 0.00010.00154 S000001234CTF8YHR191CverifiedSubunit of a complex with Ctf18p that shares some subunits withReplication Factor C and is required for sisterchromatid cohesion 0.00010.00152 S000000514STP22YCL008CverifiedComponent of the ESCRT-I complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; homologous to the mouse and humanTsg101 tumor susceptibility gene; mutantsexhibit a Class E Vps phenotype 0.00010.00152 S000004476TRM9YML014WverifiedtRNA methyltransferase, catalyzes the esterification ofmodified uridine nucleotides in tRNAs, creating5-methylcarbonylmethyluridine in tRNA(Arg3) and5-methylcarbonylmethyl-2-thiouridine intRNA(Glu); may have a role in stress response 0.00010.00152 S000004494RAD52YML032CverifiedProtein that stimulates strand exchange by facilitating Rad51pbinding to single-stranded DNA; annealscomplementary single-stranded DNA; involved inthe repair of double-strand breaks in DNAduring vegetative growth and meiosis 0.00010.00152 S000004312MMS22YLR320WverifiedProtein involved in resistance to ionizing radiation; acts withMms1p in a repair pathway that may be involvedin resolving replication intermediates orpreventing the damage caused by blockedreplication forks 0.00010.00152&radic S000000744EAF5YEL018WuncharacterizedEsa1p-associated factor, subunit of the NuA4 acetyltransferasecomplex 0.00010.0015 S000005175PDR16YNL231CverifiedPhosphatidylinositol transfer protein (PITP) controlled by themultiple drug resistance regulator Pdr1p,localizes to lipid particles and microsomes,controls levels of various lipids, may regulatelipid synthesis, homologous to Pdr17p 8e-050.00136&radic S000003854IME1YJR094CverifiedMaster regulator of meiosis that is active only during meioticevents, activates transcription of earlymeiotic genes through interaction with Ume6p,degraded by the 26S proteasome followingphosphorylation by Ime2p 8e-050.00136 S000000235RPL4AYBR031WverifiedN-terminally acetylated protein component of the large (60S)ribosomal subunit, nearly identical to Rpl4Bpand has similarity to E. coli L4 and rat L4ribosomal proteins 8e-050.00134 S000005153SSB2YNL209WverifiedCytoplasmic ATPase that is a ribosome-associated molecularchaperone, functions with J-protein partnerZuo1p; may be involved in the folding ofnewly-synthesized polypeptide chains; member ofthe HSP70 family; homolog of SSB1 8e-050.00134 S000005183LAP3YNL239WverifiedAminopeptidase of cysteine protease family, has a DNA bindingactivity and acts as bleomycin hydrolase invitro; transcription is regulated by galactosevia Gal4p 8e-050.00134 S000028574YMR030W-AUncharacterizedPutative protein of unknown function 8e-050.00134 S000001830YFL064CUncharacterizedPutative protein of unknown function 8e-050.00134 S000002309RPC53YDL150WverifiedRNA polymerase III subunit C53 8e-050.00134 S000004274ECI1YLR284CverifiedPeroxisomal delta3,delta2-enoyl-CoA isomerase, hexamericprotein that converts 3-hexenoyl-CoA totrans-2-hexenoyl-CoA, essential for thebeta-oxidation of unsaturated fatty acids,oleate-induced 8e-050.00134 S000003570HCA4YJL033WverifiedPutative nucleolar DEAD box RNA helicase; high-copy numbersuppression of a U14 snoRNA processing mutantsuggests an involvement in 18S rRNA synthesis 8e-050.00134 S000001583YKL100CUncharacterizedPutative protein of unknown function with similarity to a human minor histocompatibility antigen; YKL100C is not an essential gene 8e-050.00134 S000000016YAL018CUncharacterizedHypothetical protein 8e-050.00134 S000002315YDL156WUncharacterizedHypothetical protein 8e-050.00134 S000005335POP2YNR052CverifiedRNase of the DEDD superfamily, subunit of the Ccr4-Not complexthat mediates 3' to 5' mRNA deadenylation 8e-050.00134 S000005040RPS7BYNL096CverifiedProtein component of the small (40S) ribosomal subunit, nearlyidentical to Rps7Ap; interacts with Kti11p;deletion causes hypersensitivity to zymocin;has similarity to rat S7 and Xenopus S8ribosomal proteins 8e-050.00134 S000028566YLR159C-AUncharacterizedPutative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-5 8e-050.00134 S000002590CDC1YDR182WverifiedPutative membrane protein of unknown function involved in Mn2+homeostasis; mutants display actin and generalgrowth defects, heterogeneous cell cyclearrests, and pleiotropic defects in cell cycleprogression and organelle distribution 8e-050.00134 S000006309COG4YPR105CverifiedEssential component of the conserved oligomeric Golgi complex(Cog1p through Cog8p), a cytosolic tetheringcomplex that functions in protein traffickingto mediate fusion of transport vesicles toGolgi compartments 8e-050.00134 S000000766UTR2YEL040WverifiedPutative glycosidase, glycosylphosphatidylinositol(GPI)-anchored protein localized to the budneck; has a role in cell wall maintenance 8e-050.00134 S000005471MDY2YOL111CverifiedProtein required for efficient mating; involved in shmooformation and nuclear migration in thepre-zygote; associates with ribosomes andinteracts with YOR164C; contains aubiquitin-like (UBL) domain 8e-050.00134 S000005540RTS1YOR014WverifiedB-type regulatory subunit of protein phosphatase 2A (PP2A);homolog of the mammalian B' subunit of PP2A 8e-050.00134 S000003043MPS2YGL075CverifiedEssential membrane protein localized at the nuclear envelopeand spindle pole body (SPB), required forinsertion of the newly duplicated SPB into thenuclear envelope; potentially phosphorylated byCdc28p 8e-050.00134 S000003060NUP145YGL092WverifiedEssential nucleoporin, catalyzes its own cleavage in vivo togenerate a C-terminal fragment that assemblesinto the Nup84p subcomplex of the nuclear porecomplex, and an N-terminal fragment of unknownfunction that is homologous to Nup100p 8e-050.00134 S000001213NMD3YHR170WverifiedProtein involved in nuclear export of the large ribosomalsubunit; acts as a Crm1p-dependent adapterprotein for export of nascent ribosomalsubunits through the nuclear pore complex 8e-050.00133 S000005732NOC2YOR206WverifiedProtein that forms a nucleolar complex with Mak21p that bindsto 90S and 66S pre-ribosomes, as well as anuclear complex with Noc3p that binds to 66Spre-ribosomes; both complexes mediateintranuclear transport of ribosomal precursors 8e-050.00133 S000000555APA1YCL050CverifiedDiadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4Aphosphorylase), involved in catabolism ofbis(5'-nucleosidyl) tetraphosphates; hassimilarity to Apa2p 8e-050.00133 S000001613SHE2YKL130CverifiedRNA-binding protein that binds specific mRNAs and interactswith She3p; part of the mRNA localizationmachinery that restricts accumulation ofcertain proteins to the bud 8e-050.00133 S000005985CWC27YPL064CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to S. pombeCwf27p 8e-050.00133 S000000796DSF1YEL070WuncharacterizedDeletion suppressor of mpt5 mutation 8e-050.00133 S000004234MAP1YLR244CverifiedMethionine aminopeptidase, catalyzes the cotranslationalremoval of N-terminal methionine from nascentpolypeptides; function is partially redundantwith that of Map2p 8e-050.00133 S000000647YCR051WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene 8e-050.00133 S000028595YAR035C-AUncharacterizedIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 8e-050.00133 S000000792HPA3YEL066WverifiedD-Amino acid N-acetyltransferase, catalyzes N-acetylation ofD-amino acids through ordered bi-bi mechanismin which acetyl-CoA is first substrate boundand CoA is last product liberated; similar toHpa2p, acetylates histones weakly in vitro 8e-050.00133 S000000262UBP14YBR058CverifiedUbiquitin-specific protease that specifically disassemblesunanchored ubiquitin chains; involved infructose-1,6-bisphosphatase (Fbp1p)degradation; similar to human isopeptidase T 8e-050.00133 S000004034PDC1YLR044CverifiedMajor of three pyruvate decarboxylase isozymes, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde; subject to glucose-, ethanol-,and autoregulation; involved in amino acidcatabolism 8e-050.00133&radic S000005076KRE33YNL132WverifiedEssential protein of unknown function; heterozygous mutantshows haploinsufficiency in K1 killer toxinresistance 7e-050.00132&radic S000007488YBL059C-AUncharacterizedHypothetical protein 7e-050.00132 S000004589NDI1YML120CverifiedNADH:ubiquinone oxidoreductase, transfers electrons from NADHto ubiquinone in the respiratory chain but doesnot pump protons, in contrast to the highereukaryotic multisubunit respiratory complex I;homolog of human AMID 7e-050.00132&radic S000004835FSH2YMR222CverifiedSerine hydrolase that localizes to the cytoplasm; sequence issimilar to Fsh1p and Fsh3p 7e-050.00132 S000001837AAD16YFL057CverifiedPutative aryl-alcohol dehydrogenase with similarity to P.chrysosporium aryl-alcohol dehydrogenase;mutational analysis has not yet revealed aphysiological role 7e-050.00132 S000002794MUS81YDR386WverifiedHelix-hairpin-helix protein, involved in DNA repair andreplication fork stability; functions as anendonuclease in complex with Mms4p; interactswith Rad54p 7e-050.00132 S000004530ORC1YML065WverifiedLargest subunit of the origin recognition complex, whichdirects DNA replication by binding toreplication origins and is also involved intranscriptional silencing; may bephosphorylated by Cdc28p 7e-050.00132&radic S000002267YDL109CUncharacterizedPutative lipase; involved in lipid metabolism; YDL109C is not an essential gene 7e-050.00132 S000003059NBP35YGL091CverifiedEssential P-loop NTPase that functions in nuclear and cytosoliciron-sulfur (FeS) protein assembly; has ahighly conserved N-terminus 7e-050.00132&radic S000001646PIR3YKL163WverifiedO-glycosylated covalently-bound cell wall protein required forcell wall stability; expression is cell cycleregulated, peaking in M/G1 and also subject toregulation by the cell integrity pathway 7e-050.00132 S000005254ZIM17YNL310CverifiedHeat shock protein with a zinc finger motif; essential forprotein import into mitochondria; may act withPam18p to facilitate recognition and folding ofimported proteins by Ssc1p (mtHSP70) in themitochondrial matrix 7e-050.00132 S000000384DTR1YBR180WverifiedMultidrug resistance dityrosine transporter of the majorfacilitator superfamily, essential for sporewall synthesis, facilitates the translocationof bisformyl dityrosine through the prosporemembrane 7e-050.00132 S000002625RAD9YDR217CverifiedDNA damage-dependent checkpoint protein, required forcell-cycle arrest in G1/S, intra-S, and G2/M;transmits checkpoint signal by activatingRad53p and Chk1p; hyperphosphorylated by Mec1pand Tel1p; potential Cdc28p substrate 7e-050.00132 S000028551YGR240C-AUncharacterizedPutative protein of unknown function; identified by fungal homology and RT-PCR 7e-050.00132 S000028662YJL077W-BUncharacterizedPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 7e-050.00132 S000006120YPL199CUncharacterizedHypothetical protein 7e-050.00132 S000005278SNO2YNL334CverifiedProtein of unknown function, nearly identical to Sno3p;expression is induced before the diauxic shiftand also in the absence of thiamin 7e-050.00132 S000002393GYP7YDL234CverifiedGTPase-activating protein for yeast Rab family membersincluding: Ypt7p (most effective), Ypt1p,Ypt31p, and Ypt32p (in vitro); involved invesicle mediated protein trafficking 7e-050.00132 S000005804HEM4YOR278WverifiedUroporphyrinogen III synthase, catalyzes the conversion ofhydroxymethylbilane to uroporphyrinogen III,the fourth step in the heme biosyntheticpathway 7e-050.00132 S000003169MCM6YGL201CverifiedProtein involved in DNA replication; component of the Mcm2-7hexameric complex that binds chromatin as apart of the pre-replicative complex 7e-050.00132 S000005451SPO21YOL091WverifiedComponent of the meiotic outer plaque of the spindle pole body,involved in modifying the meiotic outer plaquethat is required prior to prospore membraneformation 7e-050.00132&radic S000028562YLR154C-HUncharacterizedPutative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-2 7e-050.00132 S000005001OCA2YNL056WuncharacterizedPutative protein with similarity to predicted tyrosinephosphatases Oca1p and Siw14p; greenfluorescent protein (GFP)-fusion proteinlocalizes to the cytoplasm; YNL056W is not anessential gene 7e-050.00132 S000005888PRT1YOR361CverifiedSubunit of the core complex of translation initiation factor3(eIF3), essential for translation; part of asubcomplex (Prt1p-Rpg1p-Nip1p) that stimulatesbinding of mRNA and tRNA(i)Met to ribosomes 7e-050.00132 S000000064SWD1YAR003WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres; WD40beta propeller superfamily member withsimilarity to mammalian Rbbp7 7e-050.00132 S000005031TCB2YNL087WverifiedBud-specific protein with a potential role in membranetrafficking; GFP-fusion protein migrates fromthe cell surface to intracellular vesicles nearvacuole; contains 3 calcium and lipid bindingdomains; mRNA is targeted to the bud via themRNA transport system involving She2p 7e-050.00132 S000007608YGR271C-AUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus 7e-050.00132 S000004570CAC2YML102WverifiedComponent of the chromatin assembly complex (with Rlf2p andMsi1p) that assembles newly synthesizedhistones onto recently replicated DNA, requiredfor building functional kinetochores, conservedfrom yeast to humans 7e-050.00132 S000003357YGR125WUncharacterizedPutative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 7e-050.00132 S000001713YKR005CUncharacterizedPutative protein of unknown function 7e-050.00132 S000005093PGA2YNL149CuncharacterizedEssential protein required for maturation of Gas1p and Pho8p;involved in protein trafficking; GFP-fusionprotein localizes to the ER and YFP-fusionprotein to the nuclear envelope-ER network;null mutants have a cell separation defect 7e-050.00132 S000005648PFY1YOR122CverifiedProfilin, actin- and phosphatidylinositol4,5-bisphosphate-binding protein, plays a rolein cytoskeleton organization, required fornormal timing of actin polymerization inresponse to thermal stress; localizes to plasmamembrane and cytosol 7e-050.00132 S000005096YNL152WUncharacterizedProtein required for cell viability 7e-050.00132 S000003823NTA1YJR062CverifiedAmidase, removes the amide group from N-terminal asparagine andglutamine residues to generate proteins withN-terminal aspartate and glutamate residuesthat are targets of ubiquitin-mediateddegradation 7e-050.00132 S000000070ADE1YAR015WverifiedN-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR)synthetase, required for 'de novo' purinenucleotide biosynthesis; red pigmentaccumulates in mutant cells deprived of adenine 7e-050.00132 S000003325YGR093WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus 7e-050.00132 S000005563CYC2YOR037WverifiedMitochondrial peripheral inner membrane protein, contains a FADcofactor in a domain exposed in theintermembrane space; exhibits redox activity invitro; likely participates in ligation of hemeto acytochromes c and c1 (Cyc1p and Cyt1p) 7e-050.00132&radic S000003884RPS5YJR123WverifiedProtein component of the small (40S) ribosomal subunit, theleast basic of the non-acidic ribosomalproteins; phosphorylated in vivo; essential forviability; has similarity to E. coli S7 and ratS5 ribosomal proteins 7e-050.00132&radic S000004744GAT2YMR136WverifiedProtein containing GATA family zinc finger motifs; similar toGln3p and Dal80p; expression repressed byleucine 7e-050.00132 S000002957TMA20YER007C-AverifiedProtein of unknown function that associates with ribosomes andhas a putative RNA binding domain; interactswith Tma22p; null mutant exhibits translationdefects; has homology to human oncogene MCT-1 7e-050.00132 S000003551CCT3YJL014WverifiedSubunit of the cytosolic chaperonin Cct ring complex, relatedto Tcp1p, required for the assembly of actinand tubulins in vivo 7e-050.00132&radic S000005122RPS3YNL178WverifiedProtein component of the small (40S) ribosomal subunit, hasapurinic/apyrimidinic (AP) endonucleaseactivity; essential for viability; hassimilarity to E. coli S3 and rat S3 ribosomalproteins 7e-050.00132 S000001081MSC7YHR039CverifiedProtein of unknown function, green fluorescent protein(GFP)-fusion protein localizes to theendoplasmic reticulum; msc7 mutants aredefective in directing meiotic recombinationevents to homologous chromatids 7e-050.00132 S000001426NIT1YIL164CverifiedNitrilase, member of the widely found nitrilase branch(EC:3.5.5.1) of the nitrilase superfamily 7e-050.00132 S000005150RTT106YNL206CverifiedProtein with a role in regulation of Ty1 transposition 7e-050.00132 S000003946YLL023CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLL023C is not an essential gene 7e-050.00132 S000001309SYG1YIL047CverifiedPlasma membrane protein of unknown function; truncation andoverexpression suppresses lethality of G-alphaprotein deficiency 7e-050.00132&radic S000000889YER087WUncharacterizedHypothetical protein 7e-050.00132 S000004507RRN11YML043CverifiedProtein required for rDNA transcription by RNA polymerase I,component of the core factor (CF) of rDNAtranscription factor, which also contains Rrn6pand Rrn7p 7e-050.00132 S000001157DMA1YHR115CverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma2p;homolog of S. pombe Dma1 and H. sapiens Chfr 7e-050.0013 S000003509YGR277CUncharacterizedPutative protein of unknown function; sequence similarity to Pantetheine-phosphate adenylyltransferase (PPAT); YGR277C is an essential gene 7e-050.0013 S000001957SAE3YHR079C-AverifiedMeiosis specific protein involved in DMC1-dependent meioticrecombination, forms heterodimer with Mei5p;proposed to be an assembly factor for Dmc1p 7e-050.0013 S000005141WHI3YNL197CverifiedRNA binding protein that sequesters CLN3 mRNA in cytoplasmicfoci; cytoplasmic retention factor for Cdc28pand associated cyclins; regulates cell fate anddose-dependently regulates the critical cellsize required for passage through Start 7e-050.0013&radic&radic S000003755HXT9YJL219WverifiedPutative hexose transporter that is nearly identical to Hxt11p,has similarity to major facilitator superfamily(MFS) transporters, expression of HXT9 isregulated by transcription factors Pdr1p andPdr3p 7e-050.0013 S000001848FMP32YFL046WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 7e-050.0013 S000001183RPL42BYHR141CverifiedProtein component of the large (60S) ribosomal subunit,identical to Rpl42Ap and has similarity to ratL44; required for propagation of the killertoxin-encoding M1 double-stranded RNA satelliteof the L-A double-stranded RNA virus 7e-050.0013 S000005822YOR296WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene 7e-050.0013 S000004059MEF1YLR069CverifiedMitochondrial elongation factor involved in translationalelongation 7e-050.0013 S000003807TAH11YJR046WverifiedDNA replication licensing factor, required for pre-replicationcomplex assembly 7e-050.0013 S000003849BIR1YJR089WverifiedEssential chromosomal passenger protein involved incoordinating cell cycle events for properchromosome segregation; C-terminal region bindsSli15p, and the middle region, uponphosphorylation, localizes Cbf2p to the spindleat anaphase 7e-050.0013 S000006138BMS1YPL217CverifiedEssential conserved nucleolar GTP-binding protein required forsynthesis of 40S ribosomal subunits and forprocessing of the 35S pre-rRNA at sites A0, A1,and A2; interacts with Rcl1p, has similarity toTsr1p 7e-050.0013 S000000809PAC2YER007WverifiedMicrotubule effector required for tubulin heterodimerformation, binds alpha-tubulin, required fornormal microtubule function, null mutantexhibits cold-sensitive microtubules andsensitivity to benomyl 7e-050.0013 S000000276HSP26YBR072WverifiedSmall heat shock protein with chaperone activity that isregulated by a heat induced transition from aninactive oligomeric (24-mer) complex to anactive dimer; induced by heat, upon entry intostationary phase, and during sporulation 7e-050.0013 S000001735BCH2YKR027WverifiedMember of the Chs5p-Arf1p-binding proteins (ChAPs), a group of4 related, interacting proteins (Bch1p, Fmp50p,Bud7p, Chs6p) that mediate export of specificcargo proteins, including chitin synthaseChs3p, from the Golgi to plasma membrane 7e-050.0013 S000005755WTM2YOR229WverifiedTranscriptional repressor involved in regulation of meiosis andsilencing; contains WD repeats 7e-050.0013 S000000903AST2YER101CverifiedProtein that may have a role in targeting of plasma membrane[H+]ATPase (Pma1p) to the plasma membrane, assuggested by analysis of genetic interactions 7e-050.0013 S000004550TUB1YML085CverifiedAlpha-tubulin; associates with beta-tubulin (Tub2p) to formtubulin dimer, which polymerizes to formmicrotubules 7e-050.0013&radic S000002435REG1YDR028CverifiedRegulatory subunit of type 1 protein phosphatase Glc7p,involved in negative regulation ofglucose-repressible genes 7e-050.0013 S000004162DPH5YLR172CverifiedMethyltransferase required for synthesis of diphthamide, whichis a modified histidine residue of translationelongation factor 2 (Eft1p or Eft2p); notessential for viability; GFP-Dph5p fusionprotein localizes to the cytoplasm 7e-050.0013 S000005202DSL1YNL258CverifiedEndoplasmic reticulum (ER)-localized peripheral membraneprotein required for Golgi-to-ER retrogradetraffic; component of the ER target site thatinteracts with coatomer, the major component ofthe COPI vesicle protein coat 7e-050.0013 S000001296CAP2YIL034CverifiedBeta subunit of the capping protein (CP) heterodimer (Cap1p andCap2p) which binds to the barbed ends of actinfilaments preventing further polymerization;localized predominantly to cortical actinpatches 7e-050.0013 S000002463YDR056CUncharacterizedHypothetical protein 7e-050.0013 S000005075TOM22YNL131WverifiedComponent of the TOM (translocase of outer membrane) complexresponsible for initial import ofmitochondrially directed proteins; acts as areceptor for precursor proteins and mediatesinteraction between the TOM and TIM complexes 7e-050.0013 S000002157NHP6BYBR089C-AverifiedHigh-mobility group non-histone chromatin protein, functionallyredundant with Nhp6Ap; homologous to mammalianhigh mobility group proteins 1 and 2; acts torecruit transcription factor Rcs1p to certainpromoters 7e-050.0013 S000003478BRF1YGR246CverifiedTFIIIB B-related factor, one of three subunits of RNApolymerase III transcription initiation factorTFIIIB, binds TFIIIC and TBP and recruits RNApol III to promoters, amino-terminal half ishomologous to TFIIB 7e-050.0013 S000000537STE50YCL032WverifiedProtein involved in mating response, invasive/filamentousgrowth, and osmotolerance, acts as an adaptorthat links G protein-associated Cdc42p-Ste20pcomplex to the effector Ste11p to modulatesignal transduction 7e-050.0013 S000002714YDR306CUncharacterizedF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain 7e-050.0013 S000002790RPP2BYDR382WverifiedRibosomal protein P2 beta, a component of the ribosomal stalk,which is involved in the interaction betweentranslational elongation factors and theribosome; regulates the accumulation of P1(Rpp1Ap and Rpp1Bp) in the cytoplasm 7e-050.0013 S000004498AMD1YML035CverifiedAMP deaminase, tetrameric enzyme that catalyzes the deaminationof AMP to form IMP and ammonia; may be involvedin regulation of intracellular adeninenucleotide pools 7e-050.0013 S000001419FMP35YIL157CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 7e-050.0013 S000004342ORM2YLR350WverifiedEvolutionarily conserved protein with similarity to Orm1p,required for resistance to agents that inducethe unfolded protein response; human orthologis located in the endoplasmic reticulum 7e-050.0013&radic S000007616YKL106C-AUncharacterizedPutative protein of unknown function; identified by homology to uncharacterized proteins in other fungi 7e-050.0013 S000000094PHO11YAR071WverifiedOne of three repressible acid phosphatases, a glycoprotein thatis transported to the cell surface by thesecretory pathway; induced by phosphatestarvation and coordinately regulated by PHO4and PHO2 7e-050.0013 S000005251MCK1YNL307CverifiedProtein serine/threonine/tyrosine (dual-specificity) kinaseinvolved in control of chromosome segregationand in regulating entry into meiosis; relatedto mammalian glycogen synthase kinases of theGSK-3 family 7e-050.0013 S000005403NTG2YOL043CverifiedDNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involvedin base excision repair, localizes to thenucleus 7e-050.0013 S000002255RPN6YDL097CverifiedEssential, non-ATPase regulatory subunit of the 26S proteasomelid required for the assembly and activity ofthe 26S proteasome; the human homolog (S9protein) partially rescues Rpn6p depletion 7e-050.0013 S000003195YGL226WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion 7e-050.0013 S000005878MEK1YOR351CverifiedMeiosis-specific serine/threonine protein kinase, functions inmeiotic checkpoint, phosphorylates Red1p,interacts with Hop1p; required for meioticrecombination and normal spore viability 7e-050.0013 S000005350DSE4YNR067CverifiedDaughter cell-specific secreted protein with similarity toglucanases, degrades cell wall from thedaughter side causing daughter to separate frommother 7e-050.0013 S000005496PFK27YOL136Cverified6-phosphofructo-2-kinase, catalyzes synthesis offructose-2,6-bisphosphate; inhibited byphosphoenolpyruvate and sn-glycerol3-phosphate, expression induced by glucose andsucrose, transcriptional regulation involvesprotein kinase A 7e-050.0013 S000001523NFU1YKL040CverifiedProtein involved in iron metabolism in mitochondria; similar toNifU, which is a protein required for thematuration of the Fe/S clusters of nitrogenasein nitrogen-fixing bacteria 7e-050.0013 S000004425CNA1YLR433CverifiedCalcineurin A; one isoform (the other is CMP2) of the catalyticsubunit of calcineurin, aCa++/calmodulin-regulated protein phosphatasewhich regulates Crz1p (a stress-responsetranscription factor), the other calcineurinsubunit is CNB1 7e-050.0013 S000006325THI22YPR121WverifiedProtein with similarity to hydroxymethylpyrimidine phosphatekinases; member of a gene family with THI20 andTHI21; not required for thiamine biosynthesis 7e-050.0013 S000006217YPR013CUncharacterizedPutative zinc finger protein; YPR013C is not an essential gene 7e-050.0013&radic S000000834FIR1YER032WverifiedProtein involved in 3' mRNA processing, interacts with Ref2p;potential Cdc28p substrate 7e-050.0013 S000006040DBP1YPL119CverifiedPutative ATP-dependent RNA helicase of the DEAD-box proteinfamily; mutants show reduced stability of the40S ribosomal subunit scanning through 5'untranslated regions of mRNAs 7e-050.0013 S000004626MRPL3YMR024WverifiedMitochondrial ribosomal protein of the large subunit 7e-050.0013 S000000057YAL061WUncharacterizedputative polyol dehydrogenase 7e-050.0013 S000004545DUS1YML080WverifiedDihydrouridine synthase, member of a widespread family ofconserved proteins including Smm1p, Dus3p, andDus4p; modifies pre-tRNA(Phe) at U17 7e-050.0013 S000004073EMP70YLR083CverifiedProtein whose 24kDa cleavage product is found inendosome-enriched membrane fractions, predictedto be a transmembrane protein 7e-050.0013&radic S000000291RFC5YBR087WverifiedSubunit of heteropentameric Replication factor C (RF-C), whichis a DNA binding protein and ATPase that actsas a clamp loader of the proliferating cellnuclear antigen (PCNA) processivity factor forDNA polymerases delta and epsilon 7e-050.0013 S000003023OLE1YGL055WverifiedFatty acid desaturase, required for monounsaturated fatty acidsynthesis and for normal distribution ofmitochondria 7e-050.0013 S000004775PAH1YMR165CverifiedMg2+-dependent phosphatidate (PA) phosphatase,catalyzes the dephosphorylation of PA to yielddiacylglycerol and Pi, responsiblefor de novo lipid synthesis; homologous tomammalian lipin 1 7e-050.0013 S000004760YIM1YMR152WverifiedProtein of unknown function; null mutant displays sensitivityto DNA damaging agents; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 7e-050.0013 S000004728RPL15BYMR121CverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl15Ap and has similarity to ratL15 ribosomal protein; binds to 5.8 S rRNA 7e-050.0013 S000003481MGA1YGR249WverifiedProtein similar to heat shock transcription factor; multicopysuppressor of pseudohyphal growth defects ofammonium permease mutants 7e-050.0013 S000001399TMA108YIL137CverifiedProtein that associates with ribosomes; putativemetalloprotease 7e-050.0013 S000028725YPR159C-AUncharacterizedIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 7e-050.0013 S000002668SWM1YDR260CverifiedSubunit of the anaphase-promoting complex, which is an E3ubiquitin ligase that regulates themetaphase-anaphase transition and exit frommitosis; required for activation of thedaughter-specific gene expression and sporewall maturation 7e-050.0013&radic S000004587NGL3YML118WverifiedPutative endonuclease, has a domain similar to amagnesium-dependent endonuclease motif in mRNAdeadenylase Ccr4p; similar to Ngl1p and Ngl2p 7e-050.0013 S000005468INO4YOL108CverifiedTranscription factor required for derepression ofinositol-choline-regulated genes involved inphospholipid synthesis; forms a complex, withIno2p, that binds theinositol-choline-responsive element through abasic helix-loop-helix domain 7e-050.0013 S000004275NNT1YLR285WverifiedPutative nicotinamide N-methyltransferase, has a role in rDNAsilencing and in lifespan determination 7e-050.0013&radic S000005682NFI1YOR156CverifiedSUMO ligase, catalyzes the covalent attachment of SUMO (Smt3p)to proteins; involved in maintenance of propertelomere length 7e-050.0013 S000000271TIP1YBR067CverifiedMajor cell wall mannoprotein with possible lipase activity;transcription is induced by heat- andcold-shock; member of the Srp1p/Tip1p family ofserine-alanine-rich proteins 7e-050.0013 S000000929LCP5YER127WverifiedEssential protein involved in maturation of 18S rRNA; depletionleads to inhibited pre-rRNA processing andreduced polysome levels; localizes primarily tothe nucleolus 7e-050.0013 S000001578YJU2YKL095WverifiedEssential nuclear protein; putative spliceosomal componentinvolved in mRNA splicing, based oncomputational analysis of large-scaleprotein-protein interaction data 7e-050.0013 S000006078TGS1YPL157WverifiedTrimethyl guanosine synthase, conserved nucleolar methyltransferase responsible for conversion of them(7)G cap structure of snRNAs and snoRNAs tom(2,2,7)G; also required for ribosome synthesisand nucleolar morphology 7e-050.0013 S000007522YBR085C-AUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus 7e-050.0013 S000002286VCX1YDL128WverifiedVacuolar H+/Ca2+ exchanger involved in control of cytosolicCa2+ concentration; has similarity tosodium/calcium exchangers, including the bovineNa+/Ca2+,K+ antiporter 7e-050.0013 S000004965ARK1YNL020CverifiedSerine/threonine protein kinase involved in regulation of thecortical actin cytoskeleton; involved incontrol of endocytosis 7e-050.0013&radic S000002408NTH1YDR001CverifiedNeutral trehalase, degrades trehalose; required forthermotolerance and may mediate resistance toother cellular stresses; may be phosphorylatedby Cdc28p 7e-050.0013 S000005724BFR1YOR198CverifiedComponent of mRNP complexes associated with polyribosomes;implicated in secretion and nuclearsegregation; multicopy suppressor of BFA(Brefeldin A) sensitivity 7e-050.0013&radic S000001895DEG1YFL001WverifiedNon-essential tRNA:pseudouridine synthase, introducespseudouridines at position 38 or 39 in tRNA,important for maintenance of translationefficiency and normal cell growth, localizes toboth the nucleus and cytoplasm 7e-050.0013 S000005140YNL196CUncharacterizedSporulation-specific protein with a leucine zipper motif 7e-050.0013 S000005561SHE4YOR035CverifiedProtein containing a UCS (UNC-45/CRO1/SHE4) domain, binds tomyosin motor domains to regulate myosinfunction; involved in endocytosis, polarizationof the actin cytoskeleton, and asymmetric mRNAlocalization 7e-050.0013 S000001506YKL023WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 7e-050.0013 S000001263YIL001WUncharacterizedHypothetical protein 7e-050.0013 S000001297CKA1YIL035CverifiedAlpha catalytic subunit of casein kinase 2, a Ser/Thr proteinkinase with roles in cell growth andproliferation; the holoenzyme also containsCKA2, CKB1 and CKB2, the many substratesinclude transcription factors and all RNApolymerases 7e-050.0013&radic S000007587KTI11YBL071W-AverifiedProtein required for synthesis of diphthamide, a modifiedhistidine residue of translation elongationfactor 2; functions with Dph1p, Dph2p, Jjj3p,and Dph5p; required, with Elongator complex,for modification of wobble nucleosides in tRNA 7e-050.0013 S000004117APC2YLR127CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; similar tocullin Cdc53p 7e-050.0013 S000001897LOC1YFR001WverifiedNuclear protein involved in asymmetric localization of ASH1mRNA; binds double-stranded RNA in vitro;constituent of 66S pre-ribosomal particles 7e-050.0013 S000002527TRM1YDR120CverifiedtRNA methyltransferase, localizes to both the nucleus andmitochondrion to produce the modified baseN2,N2-dimethylguanosine in tRNAs in bothcompartments 7e-050.0013 S000001012OPI1YHL020CverifiedTranscriptional regulator of a variety of genes;phosphorylation by protein kinase A stimulatesOpi1p function in negative regulation ofphospholipid biosynthetic genes; involved intelomere maintenance 7e-050.0013 S000003340CLB1YGR108WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 7e-050.0013 S000005250MRPS18YNL306WverifiedMitochondrial ribosomal protein of the small subunit; essentialfor viability, unlike most other mitoribosomalproteins 7e-050.0013 S000005721SLK19YOR195WverifiedKinetochore-associated protein required for normal segregationof chromosomes in meiosis and mitosis;component of the FEAR regulatory network, whichpromotes Cdc14p release from the nucleolusduring anaphase; potential Cdc28p substrate 7e-050.0013 S000000517GBP2YCL011CverifiedPoly(A+) RNA-binding protein, involved in the export of mRNAsfrom the nucleus to the cytoplasm; similar toHrb1p and Npl3p; also binds single-strandedtelomeric repeat sequence in vitro 7e-050.0013 S000003350RPS23AYGR118WverifiedRibosomal protein 28 (rp28) of the small (40S) ribosomalsubunit, required for translational accuracy;nearly identical to Rps23Bp and similar to E.coli S12 and rat S23 ribosomal proteins;deletion of both RPS23A and RPS23B is lethal 7e-050.0013 S000113559YKL023C-AUncharacterizedPutative protein of unknown function 7e-050.0013 S000003770SPC1YJR010C-AverifiedSubunit of the signal peptidase complex (SPC), which cleavesthe signal sequence from proteins targeted tothe endoplasmic reticulum (ER), homolog of theSPC12 subunit of mammalian signal peptidasecomplex 7e-050.0013 S000003246MSB2YGR014WverifiedMucin family member at the head of the Cdc42p- and MAPkinase-dependent filamentous growth signalingpathway; also functions as an osmosensor inparallel to the Sho1p-mediated pathway;potential Cdc28p substrate 7e-050.0013 S000001450STS1YIR011CverifiedProtein that interacts with the karyopherin Srp1p; may have arole with Srp1p in ubiquitin-mediated proteindegradation 7e-050.0013 S000005320RSM19YNR037CverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S19 ribosomal protein 7e-050.0013 S000003456AZR1YGR224WverifiedPlasma membrane transporter of the major facilitatorsuperfamily, involved in resistance to azoledrugs such as ketoconazole and fluconazole 7e-050.0013 S000028632YGL007C-AUncharacterizedIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 7e-050.0013 S000002548DOP1YDR141CverifiedProtein of unknown function, essential for viability, involvedin establishing cellular polarity andmorphogenesis; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 7e-050.0013 S000028579YNL130C-AUncharacterizedPutative protein of unknown function 7e-050.0013 S000004419MAG2YLR427WuncharacterizedDNA-3-methyladenine glycosidase II that catalyzes of thehydrolysis of alkylated DNA 7e-050.0013 S000003223FZF1YGL254WverifiedTranscription factor involved in sulfite metabolism, soleidentified regulatory target is SSU1,overexpression suppresses sulfite-sensitivityof many unrelated mutants due tohyperactivation of SSU1, contains five zincfingers 7e-050.0013 S000001590YKL107WUncharacterizedPutative protein of unknown function 7e-050.0013 S000006024FMP30YPL103CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 7e-050.0013&radic S000005103ASI2YNL159CverifiedIntegral inner nuclear membrane protein that acts with Asi1pand Asi3p to ensure the fidelity of SPS-sensorsignalling by maintaining the dormant repressedstate of gene expression in the absence ofinducing signals 7e-050.0013 S000001008DUR3YHL016CverifiedPlasma membrane urea transporter, expression is highlysensitive to nitrogen catabolite repression andinduced by allophanate, the last intermediateof the allantoin degradative pathway 7e-050.0013 S000006054RDS2YPL133CverifiedZinc cluster protein involved in conferring resistance toketoconazole 7e-050.0013 S000002437RAD28YDR030CverifiedProtein involved in transcription-coupled repair nucleotideexcision repair of UV-induced DNA lesions;homolog of human CSA protein 7e-050.0013 S000004264CDC46YLR274WverifiedComponent of the hexameric MCM complex, which is important forpriming origins of DNA replication in G1 andbecomes an active ATP-dependent helicase thatpromotes DNA melting and elongation whenactivated by Cdc7p-Dbf4p in S-phase 7e-050.0013 S000004990YNL045WVerifiedLeucyl aminopeptidase (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; role in vivo is not defined; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 7e-050.00129 S000004873TIF11YMR260CverifiedTranslation initiation factor eIF1A, essential protein thatforms a complex with Sui1p (eIF1) and the 40Sribosomal subunit and scans for the startcodon; C-terminus associates with Fun12p(eIF5B); N terminus interacts with eIF2 andeIF3 7e-050.00129 S000001360FMC1YIL098CverifiedMitochondrial matrix protein, required for assembly orstability at high temperature of the F1 sectorof mitochondrial F1F0 ATP synthase; null mutanttemperature sensitive growth on glycerol issuppressed by multicopy expression of Odc1p 7e-050.00129 S000000247QDR3YBR043CverifiedMultidrug transporter required for resistance to quinidine,barban, cisplatin, and bleomycin; member of themajor facilitator superfamily of transportersconferring multiple drug resistance (MFS-MDR) 7e-050.00129 S000000542SRO9YCL037CverifiedCytoplasmic RNA-binding protein that associates withtranslating ribosomes; involved in hemeregulation of Hap1p as a component of the HMCcomplex, also involved in the organization ofactin filaments; contains a La motif 7e-050.00129 S000005238RIM21YNL294CverifiedComponent of the RIM101 pathway, has a role in cell wallconstruction and alkaline pH response; hassimilarity to A. nidulans PalH 7e-050.00129 S000000273TAT1YBR069CverifiedAmino acid transport protein for valine, leucine, isoleucine,and tyrosine, low-affinity tryptophan andhistidine transporter; overexpression confersFK506 resistance 7e-050.00129 S000005390GAS5YOL030WverifiedPutative 1,3-beta-glucanosyltransferase, has similarity toGas1p; localizes to the cell wall 7e-050.00129 S000006372NUT2YPR168WverifiedComponent of the RNA polymerase II mediator complex, which isrequired for transcriptional activation andalso has a role in basal transcription 7e-050.00129 S000005283YRF1-6YNL339CverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 7e-050.00129 S000000266YBR062CUncharacterizedHypothetical protein 7e-050.00129 S000000860PET117YER058WverifiedProtein required for assembly of cytochrome c oxidase 7e-050.00129 S000003026RAD6YGL058WverifiedUbiquitin-conjugating enzyme (E2), involved in postreplicationrepair (with Rad18p), sporulation, telomeresilencing, and ubiquitin-mediated N-end ruleprotein degradation (with Ubr1p) 7e-050.00129&radic S000002999RPL24AYGL031CverifiedRibosomal protein L30 of the large (60S) ribosomal subunit,nearly identical to Rpl24Bp and has similarityto rat L24 ribosomal protein; not essential fortranslation but may be required for normaltranslation rate 7e-050.00129 S000005545YOR019WUncharacterizedHypothetical protein 7e-050.00129 S000000350MRPS9YBR146WverifiedMitochondrial ribosomal protein of the small subunit 7e-050.00129 S000005184NAR1YNL240CverifiedNuclear architecture related protein; component of thecytosolic iron-sulfur (FeS) protein assemblymachinery, required for maturation of cytosolicand nuclear FeS proteins; homologous to humanNarf 7e-050.00129 S000002161MCD1YDL003WverifiedEssential protein required for sister chromatid cohesion inmitosis and meiosis; subunit of the cohesincomplex; expression is cell cycle regulated andpeaks in S phase 7e-050.00129 S000003868YJR107WUncharacterizedPutative protein of unknown function; has sequence or structural similarity to lipases 7e-050.00129 S000000015PSK1YAL017WverifiedOne of two (see also PSK2) PAS domain containing S/T proteinkinases; coordinately regulates proteinsynthesis and carbohydrate metabolism andstorage in response to a unknown metabolitethat reflects nutritional status 7e-050.00129 S000001058YSC84YHR016CverifiedProtein involved in the organization of the actin cytoskeleton;contains SH3 domain similar to Rvs167p 7e-050.00129 S000000851TPA1YER049WuncharacterizedProtein of unknown function; interacts with Sup45p (eRF1),Sup35p (eRF3) and Pab1p; has a role intranslation termination efficiency, mRNApoly(A) tail length and mRNA stability 7e-050.00129 S000005749YOR223WUncharacterizedHypothetical protein 7e-050.00129 S000000205NTH2YBR001CverifiedPutative neutral trehalase, required for thermotolerance andmay mediate resistance to other cellularstresses 7e-050.00129 S000002263NSE4YDL105WverifiedNuclear protein that plays a role in the function of theSmc5p-Rhc18p complex 7e-050.00129 S000001028MUP3YHL036WverifiedLow affinity methionine permease, similar to Mup1p 7e-050.00129&radic S000005553STI1YOR027WverifiedHsp90 cochaperone, interacts with the Ssa group of thecytosolic Hsp70 chaperones; activates theATPase activity of Ssa1p; homolog of mammalianHop protein 7e-050.00129 S000002231YDL073WUncharacterizedPutative protein of unknown function; YDL073W is not an essential gene 7e-050.00129 S000000790AVT2YEL064CverifiedPutative transporter, member of a family of seven S. cerevisiaegenes (AVT1-7) related to vesicularGABA-glycine transporters 7e-050.00129 S000004243YLR253WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene 7e-050.00129 S000003715PFD1YJL179WverifiedSubunit of heterohexameric prefoldin, which binds cytosolicchaperonin and transfers target proteins to it;involved in the biogenesis of actin and ofalpha- and gamma-tubulin 7e-050.00129 S000028510SUS1YBR111W-AverifiedProtein involved in mRNA export coupled transcriptionactivation; component of the SAGA histoneacetylase complex 7e-050.00129 S000000863CEM1YER061CverifiedMitochondrial beta-keto-acyl synthase with possible role infatty acid synthesis; required formitochondrial respiration 7e-050.00129 S000000916BOI2YER114CverifiedProtein implicated in polar growth, functionally redundant withBoi1p; interacts with bud-emergence proteinBem1p; contains an SH3 (src homology 3) domainand a PH (pleckstrin homology) domain 7e-050.00129 S000005932TAF3YPL011CverifiedTFIID subunit (47 kDa), involved in promoter binding and RNApolymerase II transcription initiation 7e-050.00129 S000000894IES5YER092WverifiedProtein that associates with the INO80 chromatin remodelingcomplex under low-salt conditions 7e-050.00129 S000002256SNU23YDL098CverifiedComponent of U4/U6.U5 snRNP involved in mRNA splicing viaspliceosome 7e-050.00129 S000005742RUD3YOR216CverifiedGolgi matrix protein involved in the structural organization ofthe cis-Golgi; interacts genetically with COG3and USO1 7e-050.00129 S000005544ROD1YOR018WverifiedMembrane protein; overexpression confers resistance to the GSTsubstrate o-dinitrobenzene as well as to zincand calcium; contains 2 PY motifs, which arerequired for Rod1p interaction with Rsp5p, ahect-type ubiquitin ligase 7e-050.00129 S000007249YMR158C-AUncharacterizedPutative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene 7e-050.00129&radic S000002382HBT1YDL223CverifiedSubstrate of the Hub1p ubiquitin-like protein that localizes tothe shmoo tip (mating projection); mutants aredefective for mating projection formation,thereby implicating Hbt1p in polarized cellmorphogenesis 7e-050.00129 S000005574RAT1YOR048CverifiedNuclear 5' to 3' single-stranded RNA exonuclease, involved inRNA metabolism, including rRNA and snRNAprocessing as well as mRNA transcriptiontermination 7e-050.00129 S000000735GCN4YEL009CverifiedTranscriptional activator of amino acid biosynthetic genes inresponse to amino acid starvation; expressionis tightly regulated at both thetranscriptional and translational levels 7e-050.00129 S000000656TAH1YCR060WverifiedHSP90 cofactor; interacts with Hsp82p, Pih1p, Rvb1 and Rvb2,contains a single TPR domain with at least twoTPR motifs 7e-050.00129 S000004411YLR419WUncharacterizedPutative helicase with limited sequence similarity to human Rb protein; localized to the cytoplasm and the mitochondria; YLR419W is not an essential gene 7e-050.00129 S000005910FIT3YOR383CverifiedMannoprotein that is incorporated into the cell wall via aglycosylphosphatidylinositol (GPI) anchor,involved in the retention of siderophore-ironin the cell wall 7e-050.00129 S000000820SPC25YER018CverifiedComponent of the evolutionarily conservedkinetochore-associated Ndc80 complex(Ndc80p-Nuf2p-Spc24p-Spc25p); involved inchromosome segregation, spindle checkpointactivity and kinetochore clustering 7e-050.00129 S000003466YHB1YGR234WverifiedNitric oxide oxidoreductase, flavohemoglobin involved in nitricoxide detoxification; plays a role in theoxidative and nitrosative stress responses 7e-050.00129 S000003814BFA1YJR053WverifiedComponent of the GTPase-activating Bfa1p-Bub2p complex involvedin multiple cell cycle checkpoint pathways thatcontrol exit from mitosis 7e-050.00129 S000007496IGO2YHR132W-AuncharacterizedPutative protein of unknown function; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmand nucleus 7e-050.00129 S000002479IPT1YDR072CverifiedInositolphosphotransferase 1, involved in synthesis ofmannose-(inositol-P)2-ceramide (M(IP)2C), whichis the most abundant sphingolipid in cells,mutation confers resistance to the antifungalssyringomycin E and DmAMP1 in some growth media 7e-050.00129 S000004891YMR278WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR278W is not an essential gene 7e-050.00129 S000004809YMR196WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene 7e-050.00129 S000001198YSP1YHR155WuncharacterizedMitochondrial protein with a potential role in promotingmitochondrial fragmentation during programmedcell death in response to high levels ofalpha-factor mating pheromone or the drugamiodarone 7e-050.00129 S000001752UIP5YKR044WverifiedProtein of unknown function that interacts with Ulp1p, a Ubl(ubiquitin-like protein)-specific protease forSmt3p protein conjugates 7e-050.00129 S000000051PEX22YAL055WverifiedPutative peroxisomal membrane protein required for import ofperoxisomal proteins, functionally complementsa Pichia pastoris pex22 mutation 7e-050.00129&radic S000000548PDI1YCL043CverifiedProtein disulfide isomerase, multifunctional protein residentin the endoplasmic reticulum lumen, essentialfor the formation of disulfide bonds insecretory and cell-surface proteins,unscrambles non-native disulfide bonds 7e-050.00129 S000006137YPL216WUncharacterizedHypothetical protein 7e-050.00129 S000003677YAK1YJL141CverifiedSerine-threonine protein kinase that is part of aglucose-sensing system involved in growthcontrol in response to glucose availability;translocates from the cytoplasm to the nucleusand phosphorylates Pop2p in response to aglucose signal 7e-050.00129 S000002705SUR2YDR297WverifiedSphinganine C4-hydroxylase, catalyses the conversion ofsphinganine to phytosphingosine in sphingolipidbiosyntheis 7e-050.00129 S000000643BUD23YCR047CverifiedProtein involved in bud-site selection; diploid mutants displaya random budding pattern instead of thewild-type bipolar pattern 7e-050.00129 S000028558YKL183C-AUncharacterizedPutative protein of unknown function; identified by fungal homology and RT-PCR 7e-050.00129 S000005316ABZ1YNR033WverifiedPara-aminobenzoate (PABA) synthase, has similarity toEscherichia coli PABA synthase components PabAand PabB 7e-050.00129 S000005921PAU21YOR394WuncharacterizedHypothetical protein 7e-050.00129 S000005368COQ10YOL008WverifiedCoenzyme Q binding protein, required for function of coenzyme Qin respiration by functioning in the deliveryof Q6 to its proper location forelectron transport, START domain protein withhomologs in bacteria and eukaryotes 7e-050.00129 S000001185RPC10YHR143W-AverifiedRNA polymerase subunit, found in RNA polymerase complexes I,II, and III 7e-050.00129 S000003317RPL11BYGR085CverifiedProtein component of the large (60S) ribosomal subunit, nearlyidentical to Rpl11Ap; involved in ribosomalassembly; depletion causes degradation ofproteins and RNA of the 60S subunit; hassimilarity to E. coli L5 and rat L11 7e-050.00129 S000000493SNF5YBR289WverifiedSubunit of the SWI/SNF chromatin remodeling complex involved intranscriptional regulation; functionsinterdependently in transcriptional activationwith Snf2p and Snf6p 7e-050.00129&radic S000002221YDL063CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL063C is not an essential gene 7e-050.00129 S000000933RPS26BYER131WverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps26Ap and has similarity to ratS26 ribosomal protein 7e-050.00129 S000001272DOT5YIL010WverifiedNuclear thiol peroxidase which functions as analkyl-hydroperoxide reductase duringpost-diauxic growth 7e-050.00129&radic S000005088YNL144CUncharacterizedPutative protein of unknown function found in mitochondria; YNL144C is not an essential gene 7e-050.00129 S000004369FBP1YLR377CverifiedFructose-1,6-bisphosphatase, key regulatory enzyme in thegluconeogenesis pathway, required for glucosemetabolism 7e-050.00129 S000004321REC102YLR329WverifiedProtein involved in early stages of meiotic recombination;required for chromosome synapsis; forms acomplex with Rec104p and Spo11p necessaryduring the initiation of recombination 7e-050.00129 S000003899IML1YJR138WverifiedProtein of unknown function, green fluorescent protein(GFP)-fusion protein localizes to the vacuolarmembrane 7e-050.00129 S000005196MRPL17YNL252CverifiedMitochondrial ribosomal protein of the large subunit 7e-050.00129 S000001690YKL207WUncharacterizedHypothetical protein 7e-050.00129&radic S000003825CCT5YJR064WverifiedSubunit of the cytosolic chaperonin Cct ring complex, relatedto Tcp1p, required for the assembly of actinand tubulins in vivo 7e-050.00129 S000000788NPR2YEL062WverifiedProtein with a possible role in regulating expression ofnitrogen permeases; transcription is induced inresponse to proline and urea; contains two PESTsequences; null mutant is resistant tocisplatin and doxorubicin 7e-050.00129 S000004795SSO2YMR183CverifiedPlasma membrane t-SNARE involved in fusion of secretoryvesicles at the plasma membrane; syntaxinhomolog that is functionally redundant withSso1p 7e-050.00129 S000087085YBR201C-AUncharacterizedPutative protein of unknown function 7e-050.00129 S000004497SRC1YML034WverifiedProtein with a putative role in sister chromatid segregation,potentially phosphorylated by Cdc28p; greenfluorescent protein (GFP)-fusion proteinlocalizes to the nuclear periphery 7e-050.00129&radic S000001136HXT1YHR094CverifiedLow-affinity glucose transporter of the major facilitatorsuperfamily, expression is induced by Hxk2p inthe presence of glucose and repressed by Rgt1pwhen glucose is limiting 7e-050.00129 S000002342YDL183CUncharacterizedPutative protein of unknown function; YBR063C is not an essential gene 7e-050.00129 S000004333SPO77YLR341WverifiedMeiosis-specific protein of unknown function, required forspore wall formation during sporulation;dispensable for both nuclear divisions duringmeiosis 7e-050.00129 S000005107RIA1YNL163CverifiedCytoplasmic GTPase involved in biogenesis of the 60S ribosome;has similarity to translation elongation factor2 (Eft1p and Eft2p) 7e-050.00129 S000005231SEC21YNL287WverifiedGamma subunit of coatomer, a heptameric protein complex thattogether with Arf1p forms the COPI coat;involved in ER to Golgi transport of selectivecargo 7e-050.00129 S000000877PTP3YER075CverifiedPhosphotyrosine-specific protein phosphatase involved in theinactivation of mitogen-activated proteinkinase (MAPK) during osmolarity sensing;dephosporylates Hog1p MAPK and regulates itslocalization; localized to the cytoplasm 7e-050.00129 S000002725HIM1YDR317WverifiedProtein of unknown function involved in DNA repair 7e-050.00129&radic S000006009YPL088WUncharacterizedPutative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance 7e-050.00129 S000028541YDR194W-AUncharacterizedPutative protein of unknown function; identified by fungal homology and RT-PCR 7e-050.00129 S000000454SPO23YBR250WuncharacterizedProtein of unknown function; associates with meiosis-specificprotein Spo1p 7e-050.00129 S000004038RPS0BYLR048WverifiedProtein component of the small (40S) ribosomal subunit, nearlyidentical to Rps0Ap; required for maturation of18S rRNA along with Rps0Ap; deletion of eitherRPS0 gene reduces growth rate, deletion of bothgenes is lethal 7e-050.00129 S000001398OM45YIL136WverifiedProtein of unknown function, major constituent of themitochondrial outer membrane; located on theouter (cytosolic) face of the outer membrane 7e-050.00129 S000000222GAL7YBR018CverifiedGalactose-1-phosphate uridyl transferase, synthesizesglucose-1-phosphate and UDP-galactose fromUDP-D-glucose and alpha-D-galactose-1-phosphatein the second step of galactose catabolism 7e-050.00129 S000000970CCA1YER168CverifiedATP (CTP):tRNA-specific tRNA nucleotidyltransferase; differentforms targeted to the nucleus, cytosol, andmitochondrion are generated via the use ofmultiple transcriptional and translationalstart sites 7e-050.00129 S000004685NAM7YMR080CverifiedATP-dependent RNA helicase of the SFI superfamily, required fornonsense mediated mRNA decay and for efficienttranslation termination at nonsense codons;involved in telomere maintenance 7e-050.00129 S000005081NAM9YNL137CverifiedMitochondrial ribosomal component of the small subunit 7e-050.00129 S000003284FMP48YGR052WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 7e-050.00129 S000005897MRS6YOR370CverifiedRab escort protein, forms a complex with the Ras-like smallGTPase Ypt1p that is required for theprenylation of Ypt1p by proteingeranylgeranyltransferase type II (Bet2p-Bet4p) 7e-050.00129 S000004517YML053CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YML053C is not an essential gene 7e-050.00129 S000005759KIN4YOR233WverifiedKinase that acts by inhibiting the mitotic exit network (MEN)when the spindle position checkpoint isactivated; localized asymmetrically to mothercell cortex, spindle pole body and bud neck 7e-050.00129 S000002473YDR066CUncharacterizedHypothetical protein 7e-050.00129 S000006146YPL225WUncharacterizedHypothetical protein 7e-050.00129 S000003480SOL4YGR248Wverified6-phosphogluconolactonase with similarity to Sol3p 7e-050.00129 S000003135PMR1YGL167CverifiedHigh affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ andMn2+ transport into Golgi; involved in Ca2+dependent protein sorting and processing;mutations in human homolog ATP2C1 causeacantholytic skin condition Hailey-Haileydisease 7e-050.00129&radic S000000485DUG2YBR281CuncharacterizedProtein involved in alternative pathway of glutathionedegradation; green fluorescent protein(GFP)-fusion protein localizes to the cytoplasmand nucleus; YBR281C is not an essential gene 7e-050.00129 S000001624SDH3YKL141WverifiedCytochrome b subunit of succinate dehydrogenase (Sdh1p, Sdh2p,Sdh3p, Sdh4p), which couples the oxidation ofsuccinate to the transfer of electrons toubiquinone 7e-050.00129 S000002749YDR341CVerifiedCytoplasmic arginyl-tRNA synthetase 7e-050.00129 S000004511GSF2YML048WverifiedER localized integral membrane protein that may promotesecretion of certain hexose transporters,including Gal2p; involved in glucose-dependentrepression 7e-050.00129 S000001011APM2YHL019CverifiedProtein of unknown function, homologous to the medium chain ofmammalian clathrin-associated protein complex;involved in vesicular transport 7e-050.00129 S000028573YML054C-AUncharacterizedPutative protein of unknown function 7e-050.00129 S000002241RPS16BYDL083CverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps16Ap and has similarity to E.coli S9 and rat S16 ribosomal proteins 7e-050.00129 S000005383IFM1YOL023WverifiedMitochondrial translation initiation factor 2 7e-050.00129 S000003835MOG1YJR074WverifiedConserved nuclear protein that interacts with GTP-Gsp1p, whichis a Ran homolog of the Ras GTPase family, andstimulates nucleotide release, involved innuclear protein import, nucleotide release isinhibited by Yrb1p 7e-050.00129 S000001563VMA5YKL080WverifiedSubunit C of the eight-subunit V1 peripheral membrane domain ofvacuolar H+-ATPase (V-ATPase), an electrogenicproton pump found throughout the endomembranesystem; required for the V1 domain to assembleonto the vacuolar membrane 7e-050.00129 S000000091YAR068WUncharacterizedputative membrane protein 7e-050.00129 S000002727YDR319CUncharacterizedHypothetical protein 7e-050.00129 S000006153SSO1YPL232WverifiedPlasma membrane t-SNARE involved in fusion of secretoryvesicles at the plasma membrane and in vesiclefusion during sporulation; forms a complex withSec9p that binds v-SNARE Snc2p; syntaxinhomolog; functionally redundant with Sso2p 7e-050.00129 S000005981LPE10YPL060WverifiedMitochondrial inner membrane magnesium transporter, involved inmaintenance of magnesium concentrations insidemitochondria; indirectly affects splicing ofgroup II introns; functionally and structurallyrelated to Mrs2p 7e-050.00129 S000002191SLM3YDL033CuncharacterizedtRNA-specific 2-thiouridylase, responsible for 2-thiolation ofthe wobble base of mitochondrial tRNAs; humanortholog is implicated in myoclonus epilepsyassociated with ragged red fibers (MERRF) 7e-050.00129 S000005477RRI2YOL117WverifiedSubunit of the COP9 signalosome (CSN) complex that cleaves theubiquitin-like protein Nedd8 from SCF ubiquitinligases; plays a role in the mating pheromoneresponse 7e-050.00129 S000000233CDS1YBR029CverifiedPhosphatidate cytidylyltransferase (CDP-diglyceridesynthetase); an enzyme that catalyzes thatconversion of CTP + phosphate into diphosphate+ CDP-diaclglyerol, a critical step in thesynthesis of all major yeast phospholipids 7e-050.00129 S000004756YMR148WUncharacterizedPutative protein of unknown function; predicted to contain a transmembrane domain; YMR148W is not an essential gene 7e-050.00129 S000006062YPL141CUncharacterizedHypothetical protein 7e-050.00129 S000004007MEU1YLR017WverifiedMethylthioadenosine phosphorylase (MTAP), catalyzes the initialstep in the methionine salvage pathway; affectspolyamine biosynthesis through regulation ofornithine decarboxylase (Spe1p) activity;regulates ADH2 gene expression 7e-050.00129 S000000249GIP1YBR045CverifiedMeiosis-specific regulatory subunit of the Glc7p proteinphosphatase, regulates spore wall formation andseptin organization, required for expression ofsome late meiotic genes and for normallocalization of Glc7p 7e-050.00129 S000005959MET31YPL038WverifiedZinc-finger DNA-binding protein, involved in regulatingexpression of the methionine biosyntheticgenes, similar to Met32p 7e-050.00129 S000001196SPO16YHR153CverifiedProtein of unknown function, required for spore formation 7e-050.00129 S000001113PCL5YHR071WverifiedCyclin, interacts with Pho85p cyclin-dependent kinase (Cdk),induced by Gcn4p at level of transcription,specifically required for Gcn4p degradation,may be sensor of cellular protein biosyntheticcapacity 7e-050.00129 S000003674TIF2YJL138CverifiedTranslation initiation factor eIF4A, identical to Tif1p;DEA(D/H)-box RNA helicase that couples ATPaseactivity to RNA binding and unwinding; forms adumbbell structure of two compact domainsconnected by a linker; interacts with eIF4G 7e-050.00129 S000003287MUP1YGR055WverifiedHigh affinity methionine permease, integral membrane proteinwith 13 putative membrane-spanning regions;also involved in cysteine uptake 7e-050.00129 S000003757FSP2YJL221CverifiedProtein of unknown function, expression is induced duringnitrogen limitation 7e-050.00129 S000002942FIT1YDR534CverifiedMannoprotein that is incorporated into the cell wall via aglycosylphosphatidylinositol (GPI) anchor,involved in the retention of siderophore-ironin the cell wall 7e-050.00129 S000000159KIP1YBL063WverifiedKinesin-related motor protein required for mitotic spindleassembly and chromosome segregation;functionally redundant with Cin8p 7e-050.00129 S000004514YML050WUncharacterizedHypothetical protein 7e-050.00129 S000005650UBP2YOR124CverifiedUbiquitin-specific protease that removes ubiquitin fromubiquitinated proteins, cleaves at the Cterminus of ubiquitin fusions; capable ofcleaving polyubiquitin and possessesisopeptidase activity 7e-050.00129 S000006233APL4YPR029CverifiedGamma-adaptin, large subunit of the clathrin-associated protein(AP-1) complex; binds clathrin; involved invesicle mediated transport 7e-050.00129 S000002596CCT6YDR188WverifiedSubunit of the cytosolic chaperonin Cct ring complex, relatedto Tcp1p, essential protein that is requiredfor the assembly of actin and tubulins in vivo;contains an ATP-binding motif 7e-050.00129 S000005711GSP2YOR185CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; interacts withKap121p, Kap123p and Pdr6p (karyophilin betas);Gsp1p homolog that is not required forviability 7e-050.00129 S000000370TYR1YBR166CverifiedPrephenate dehydrogenase involved in tyrosine biosynthesis,expression is dependent on phenylalanine levels 7e-050.00129 S000004533ITT1YML068WverifiedProtein that modulates the efficiency of translationtermination, interacts with translation releasefactors eRF1 (Sup45p) and eRF3 (Sup35p) invitro, contains a zinc finger domaincharacteristic of the TRIAD class of proteins 7e-050.00129 S000005901ALD4YOR374WverifiedMitochondrial aldehyde dehydrogenase, required for growth onethanol and conversion of acetaldehyde toacetate; activity is K+ dependent; utilizesNADP+ or NAD+ equally as coenzymes; expressionis glucose repressed 7e-050.00129 S000006209HAL1YPR005CverifiedCytoplasmic protein involved in halotolerance; decreasesintracellular Na+ (via Ena1p) and increasesintracellular K+ by decreasing efflux;expression repressed by Ssn6p-Tup1p and Sko1pand induced by NaCl, KCl, and sorbitol throughGcn4p 7e-050.00129 S000001854YFL040WUncharacterizedPutative protein of unknown function; YFL040W is not an essential gene 7e-050.00129 S000005523YOL163WUncharacterizedHypothetical protein, member of the Dal5p subfamily of the major facilitator family 7e-050.00129 S000005407YOL047CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 7e-050.00129 S000004656FAR3YMR052WverifiedProtein involved in G1 cell cycle arrest in response topheromone, in a pathway different from theFar1p-dependent pathway; interacts with Far7p,Far8p, Far9p, Far10p, and Far11p 7e-050.00129 S000004048SHM2YLR058CverifiedCytosolic serine hydroxymethyltransferase, involved inone-carbon metabolism 7e-050.00129 S000004266DBP9YLR276CverifiedATP-dependent RNA helicase of the DEAD-box family involved inbiogenesis of the 60S ribosomal subunit 7e-050.00129 S000003631BCK1YJL095WverifiedMitogen-activated protein (MAP) kinase kinase kinase acting inthe protein kinase C signaling pathway, whichcontrols cell integrity; upon activation byPkc1p phosphorylates downstream kinases Mkk1pand Mkk2p 7e-050.00129 S000002941HSP31YDR533CverifiedPossible chaperone and cysteine protease with similarity to E.coli Hsp31 and S. cerevisiae Hsp32p, Hsp33p,and Sno4p; member of the DJ-1/ThiJ/PfpIsuperfamily, which includes human DJ-1 involvedin Parkinson's disease; exists as a dimer 7e-050.00129 S000001062YHR020WUncharacterizedPutative protein of unknown function, has similarity to proline-tRNA ligase; YHR020W is an essential gene 7e-050.00129 S000005401NOP12YOL041CverifiedNucleolar protein, required for pre-25S rRNA processing;contains an RNA recognition motif (RRM) and hassimilarity to Nop13p, Nsr1p, and putativeorthologs in Drosophila and S. pombe 7e-050.00129 S000007246TMA7YLR262C-AverifiedProtein of unknown that associates with ribosomes; null mutantexhibits translation defects, alteredpolyribosome profiles, and resistance to thetranslation inhibitor anisomcyin 7e-050.00129 S000002572TRM82YDR165WverifiedSubunit of a tRNA methyltransferase complex composed of Trm8pand Trm82p that catalyzes 7-methylguanosinemodification of tRNA 7e-050.00129 S000004228FAR10YLR238WverifiedProtein involved in G1 cell cycle arrest in response topheromone, in a pathway different from theFar1p-dependent pathway; interacts with Far3p,Far7p, Far8p, Far9p, and Far11p; potentialCdc28p substrate 7e-050.00129 S000000956OXA1YER154WverifiedTranslocase of the mitochondrial inner membrane, mediates theinsertion of both mitochondrial- andnuclear-encoded proteins from the matrix intothe inner membrane, interacts withmitochondrial ribosomes; null is respiratorydeficient 7e-050.00129 S000001171ARP1YHR129CverifiedActin-related protein of the dynactin complex; required forspindle orientation and nuclear migration;putative ortholog of mammalian centractin 7e-050.00129 S000001443DJP1YIR004WverifiedCytosolic J-domain-containing protein, required for peroxisomalprotein import and involved in peroxisomeassembly, homologous to E. coli DnaJ 7e-050.00129&radic S000006052RPL5YPL131WverifiedProtein component of the large (60S) ribosomal subunit withsimilarity to E. coli L18 and rat L5 ribosomalproteins; binds 5S rRNA and is required for 60Ssubunit assembly 7e-050.00129 S000006326AXL1YPR122WverifiedHaploid specific endoprotease that performs one of twoN-terminal cleavages during maturation ofa-factor mating pheromone; required for axialbudding pattern of haploid cells 7e-050.00129 S000004541WAR1YML076CverifiedHomodimeric Zn2Cys6 zinc finger transcription factor; binds toa weak acid response element to inducetranscription of PDR12 and FUN34, encoding anacid transporter and a putative ammoniatransporter, respectively 7e-050.00129 S000004938FET4YMR319CverifiedLow-affinity Fe(II) transporter of the plasma membrane 7e-050.00129 S000004036YLR046CUncharacterizedPutative membrane protein, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance 7e-050.00129 S000007626YOL086W-AUncharacterizedHypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36. 7e-050.00129 S000003699YJL163CUncharacterizedHypothetical protein 7e-050.00129&radic S000006362YPR158WUncharacterizedHypothetical protein 7e-050.00129 S000002448RSM10YDR041WverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S10 ribosomal protein;essential for viability, unlike most othermitoribosomal proteins 7e-050.00129 S000000416NGR1YBR212WverifiedRNA binding protein that negatively regulates growth rate;interacts with the 3' UTR of the mitochondrialporin (POR1) mRNA and enhances its degradation;overexpression impairs mitochondrial function;expressed in stationary phase 7e-050.00129&radic S000001633MCR1YKL150WverifiedMitochondrial NADH-cytochrome b5 reductase, involved inergosterol biosynthesis 7e-050.00129 S000002386HOYDL227CverifiedSite-specific endonuclease required for gene conversion at theMAT locus (homothallic switching) through thegeneration of a ds DNA break; expressionrestricted to mother cells in late G1 ascontrolled by Swi4p-Swi6p, Swi5p and Ash1p 7e-050.00129 S000005838HSD1YOR311CverifiedEndoplasmic reticulum (ER)-resident membrane protein,overproduction induces enlargement of ER-likemembrane structures and suppresses atemperature-sensitive sly1 mutation 7e-050.00129 S000004345BUD8YLR353WverifiedProtein involved in bud-site selection; diploid mutants displaya unipolar budding pattern instead of thewild-type bipolar pattern, and bud at theproximal pole 7e-050.00129 S000005060DMA2YNL116WverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma1p;homolog of S. pombe Dma1 and H. sapiens Chfr 7e-050.00129 S000002218TSR1YDL060WverifiedProtein required for processing of 20S pre-rRNA in thecytoplasm, associates with pre-40S ribosomalparticles 7e-050.00129 S000002180GPD1YDL022WverifiedNAD-dependent glycerol-3-phosphate dehydrogenase, key enzyme ofglycerol synthesis, essential for growth underosmotic stress; expression regulated byhigh-osmolarity glycerol response pathway;homolog of Gpd2p 7e-050.00129&radic S000004519SPC2YML055WverifiedSubunit of signal peptidase complex (Spc1p, Spc2p, Spc3p,Sec11p), which catalyzes cleavage of N-terminalsignal sequences of proteins targeted to thesecretory pathway; homologous to mammalianSPC25 7e-050.00129 S000004442HMG2YLR450WverifiedOne of two isozymes of HMG-CoA reductase that convert HMG-CoAto mevalonate, a rate-limiting step in sterolbiosynthesis; overproduction induces assemblyof peripheral ER membrane arrays and shortnuclear-associated membrane stacks 7e-050.00129 S000002741YDR333CUncharacterizedHypothetical protein 7e-050.00129&radic S000004856ZRC1YMR243CverifiedVacuolar membrane zinc transporter, transports zinc from thecytosol into the vacuole for storage; also hasa role in resistance to zinc shock resultingfrom a sudden influx of zinc into the cytoplasm 7e-050.00129 S000005792PNT1YOR266WverifiedMitochondrial inner membrane protein involved in export ofproteins from the mitochondrial matrix;overexpression of PNT1 confers resistance tothe anti-Pneumocystis carinii drug pentamidine,and deletion confers increased sensitivity 7e-050.00129 S000004551ALO1YML086CverifiedD-Arabinono-1,4-lactone oxidase, catalyzes the final step inbiosynthesis of D-erythroascorbic acid, whichis protective against oxidative stress 7e-050.00129 S000000403KTR4YBR199WverifiedPutative mannosyltransferase involved in protein glycosylation;member of the KRE2/MNT1 mannosyltransferasefamily 7e-050.00129&radic S000005055CYB5YNL111CverifiedCytochrome b5, involved in the sterol and lipid biosynthesispathways; required for sterol C5-6 and fattyacid desaturation 7e-050.00129 S000028698YNL024C-AUncharacterizedPutative protein of unknown function; YNL024C-A is an essential gene 7e-050.00129 S000003662NIT2YJL126WverifiedNit protein, one of two proteins in S. cerevisiae withsimilarity to the Nit domain of NitFhit fromfly and worm and to the mouse and human Nitprotein which interacts with the Fhit tumorsuppressor; nitrilase superfamily member 7e-050.00129 S000003528YRF1-3YGR296WverifiedHelicase encoded by the Y' element of subtelomeric regions,highly expressed in the mutants lacking thetelomerase component TLC1; potentiallyphosphorylated by Cdc28p 7e-050.00129 S000003971YBT1YLL048CverifiedTransporter of the ATP-binding cassette (ABC) family involvedin bile acid transport; similar to mammalianbile transporters 7e-050.00129 S000002266KIN28YDL108WverifiedSerine/threonine protein kinase, subunit of the transcriptionfactor TFIIH; involved in transcriptioninitiation at RNA polymerase II promoters 7e-050.00129 S000004623MAC1YMR021CverifiedCopper-sensing transcription factor involved in regulation ofgenes required for high affinity coppertransport 7e-050.00129 S000004165CBF5YLR175WverifiedPseudouridine synthase catalytic subunit of box H/ACA smallnucleolar ribonucleoprotein particles(snoRNPs), acts on both large and small rRNAsand on snRNA U2 7e-050.00129&radic S000001504MAK11YKL021CverifiedProtein essential for cell growth and replication of M dsRNAvirus; contains four beta-transducin repeats 7e-050.00129&radic S000003510CWC22YGR278WverifiedEssential protein, component of a complex containing Cef1p, hassimilarity to S. pombe Cwf22p; putativespliceosomal component, based on computationalanalysis of large-scale protein-proteininteraction data 7e-050.00129 S000001440SGN1YIR001CverifiedCytoplasmic RNA-binding protein, contains an RNA recognitionmotif (RRM); may have a role in mRNAtranslation, as suggested by geneticinteractions with genes encoding proteinsinvolved in translational initiation 7e-050.00129 S000003954GPI13YLL031CverifiedER membrane localized phosphoryltransferase that addsphosphoethanolamine onto the third mannoseresidue of the glycosylphosphatidylinositol(GPI) anchor precursor; similar to human PIG-Oprotein 7e-050.00129&radic S000001926MET10YFR030WverifiedSubunit alpha of assimilatory sulfite reductase, which isresponsible for the conversion of sulfite intosulfide 7e-050.00129 S000005008YDJ1YNL064CverifiedProtein chaperone involved in regulation of the HSP90 and HSP70functions; involved in protein translocationacross membranes; member of the DnaJ family 7e-050.00129&radic S000004372CSR1YLR380WverifiedPhosphatidylinositol transfer protein with a potential role inlipid turnover; interacts specifically withthioredoxin peroxidase (Tsa2p) and may have arole in oxidative stress resistance 7e-050.00129&radic S000007265AI5_ALPHAQ0070verifiedEndonuclease I-SceIV, involved in intron mobility; encoded by amobile group I intron within the mitochondrialCOX1 gene 7e-050.00129 S000004153MAS1YLR163CverifiedSmaller subunit of the mitochondrial processing protease (MPP),essential processing enzyme that cleaves theN-terminal targeting sequences frommitochondrially imported proteins 7e-050.00129 S000002692DPP1YDR284CverifiedDiacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulatedvacuolar membrane-associated lipid phosphatase,dephosphorylates DGPP to phosphatidate (PA) andPi, then PA to diacylglycerol; involved inlipid signaling and cell metabolism 7e-050.00129 S000000339CKS1YBR135WverifiedSubunit of the Cdc28 protein kinase, required for mitoticproteolysis, may also be involved in theproteolysis of the G1 cyclins 7e-050.00129 S000002813MRP20YDR405WverifiedMitochondrial ribosomal protein of the large subunit 7e-050.00129 S000006010RLM1YPL089CverifiedMADS-box transcription factor, component of the protein kinaseC-mediated MAP kinase pathway involved in themaintenance of cell integrity; phosphorylatedand activated by the MAP-kinase Slt2p 7e-050.00129 S000001408ECM37YIL146CverifiedNon-essential protein of unknown function 7e-050.00129 S000005938IRC15YPL017CuncharacterizedPutative S-adenosylmethionine-dependent methyltransferase ofthe seven beta-strand family; null mutantdisplays increased levels of spontaneous Rad52foci 7e-050.00129 S000003417TYS1YGR185CverifiedCytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNAsynthetase that aminoacylates tRNA(Tyr),required for cytoplasmic protein synthesis,interacts with positions 34 and 35 of theanticodon of tRNATyr 7e-050.00129 S000000887YER085CUncharacterizedHypothetical protein 7e-050.00129 S000004318YLR326WUncharacterizedHypothetical protein 7e-050.00129 S000001785YKR077WUncharacterizedPutative transcriptional activator, identified by high-throughput two-hybrid experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YKR077W is not an essential gene 7e-050.00129 S000003176SIP2YGL208WverifiedOne of three beta subunits of the Snf1 serine/threonine proteinkinase complex involved in the response toglucose starvation; null mutants exhibitaccelerated aging; N-myristoylprotein localizedto the cytoplasm and the plasma membrane 7e-050.00129 S000006125HRR25YPL204WverifiedProtein kinase involved in regulating diverse events includingvesicular trafficking, gene expression, DNArepair, and chromosome segregation; binds theCTD of RNA pol II; homolog of mammalian caseinkinase 1delta (CK1delta) 7e-050.00129&radic S000005330YNR047WUncharacterizedPutative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; potential Cdc28p substrate 7e-050.00129 S000002786LSM6YDR378CverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 7e-050.00129 S000004247YLR257WUncharacterizedHypothetical protein 7e-050.00129 S000000988YER186CUncharacterizedHypothetical protein 7e-050.00129 S000005331YNR048WVerifiedProtein proposed to interact with phospholipid translocases, shares similarity to Cdc50p 7e-050.00129 S000000876RPS24AYER074WverifiedProtein component of the small (40S) ribosomal subunit;identical to Rps24Bp and has similarity to ratS24 ribosomal protein 7e-050.00129 S000028837YIR018C-AUncharacterizedPutative protein of unknown function, identified by expression profiling and mass spectrometry 7e-050.00129 S000004065RPL10YLR075WverifiedProtein component of the large (60S) ribosomal subunit,responsible for joining the 40S and 60Ssubunits; regulates translation initiation; hassimilarity to rat L10 ribosomal protein and tomembers of the QM gene family 7e-050.00129 S000004608PLB2YMR006CverifiedPhospholipase B (lysophospholipase) involved in phospholipidmetabolism; displays transacylase activity invitro; overproduction confers resistance tolysophosphatidylcholine 7e-050.00129 S000000543ATG22YCL038CverifiedProtein required for the breakdown of autophagic vesicles inthe vacuole during autophagy, putative integralmembrane protein that localizes to vacuolarmembranes and punctate structures attached tothe vacuole 7e-050.00129 S000003245SNU71YGR013WverifiedComponent of U1 snRNP required for mRNA splicing viaspliceosome; yeast specific, no metazoancounterpart 7e-050.00129&radic S000004667RIM9YMR063WverifiedProtein of unknown function, involved in the proteolyticactivation of Rim101p in response to alkalinepH; has similarity to A. nidulans PalI;putative membrane protein 7e-050.00129 S000004492YML030WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YML030W is not an essential gene 7e-050.00129 S000000077YAR029WUncharacterizedMember of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 7e-050.00129 S000004605YMR003WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria; YMR003W is not an essential gene 7e-050.00129 S000000905SSA4YER103WverifiedHeat shock protein that is highly induced upon stress; plays arole in SRP-dependent cotranslationalprotein-membrane targeting and translocation;member of the HSP70 family; cytoplasmic proteinthat concentrates in nuclei upon starvation 7e-050.00129 S000001194YHR151CUncharacterizedPutative protein of unknown function 7e-050.00129 S000005734PTP2YOR208WverifiedPhosphotyrosine-specific protein phosphatase involved in theinactivation of mitogen-activated proteinkinase (MAPK) during osmolarity sensing;dephosporylates Hog1p MAPK and regulates itslocalization; localized to the nucleus 7e-050.00129 S000000366TOS1YBR162CuncharacterizedCovalently-bound cell wall protein of unknown function;identified as a cell cycle regulated SBF targetgene; deletion mutants are highly resistant totreatment with beta-1,3-glucanase; has sequencesimilarity to YJL171C 7e-050.00129 S000005381DIS3YOL021CverifiedNucleolar exosome component, involved in rRNA processing andRNA degradation, binds Gsp1p/Ran and enhancesthe GEF activity of Srm1p, implicated inmitotic control, homologous to the E. coliRNase R of the RNase II family 7e-050.00129 S000003984MMP1YLL061WverifiedHigh-affinity S-methylmethionine permease, required forutilization of S-methylmethionine as a sulfursource; has similarity to S-adenosylmethioninepermease Sam3p 7e-050.00129 S000002305LDB17YDL146WverifiedProtein of unknown function; GFP-fusion protein localizes tothe cell periphery, cytoplasm, bud, and budneck; null mutant shows a reduced affinity forthe alcian blue dye suggesting a decreased netnegative charge of the cell surface 7e-050.00129 S000001030CBP2YHL038CverifiedMitochondrial protein required for splicing of the group Iintron aI5 of the COB pre-mRNA, binds to theRNA to promote splicing; also involved in butnot essential for splicing of the COB bI2intron and the intron in the 21S rRNA gene 7e-050.00129 S000006168YPL247CUncharacterizedHypothetical protein 7e-050.00128 S000006253ATG11YPR049CverifiedPeripheral membrane protein required for delivery ofaminopeptidase I (Lap4p) to the vacuole in thecytoplasm-to-vacuole targeting pathway; alsorequired for peroxisomal degradation(pexophagy) 7e-050.00128 S000002868TFB3YDR460WverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, involved in transcriptioninitiation, required for nucleotide excisionrepair; ring finger protein similar tomammalian CAK and TFIIH subunit 7e-050.00128 S000001584HSL1YKL101WverifiedNim1p-related protein kinase that regulates the morphogenesisand septin checkpoints; associates with theassembled septin filament; required along withHsl7p for bud neck recruitment,phosphorylation, and degradation of Swe1p 7e-050.00128 S000002534ARO1YDR127WverifiedPentafunctional arom protein, catalyzes steps 2 through 6 inthe biosynthesis of chorismate, which is aprecursor to aromatic amino acids 7e-050.00128 S000002803SXM1YDR395WverifiedNuclear transport factor (karyopherin) involved in proteintransport between the cytoplasm andnucleoplasm; similar to Nmd5p, Cse1p, Lph2p,and the human cellular apoptosis susceptibilityprotein, CAS1 7e-050.00128 S000000909GLE2YER107CverifiedComponent of the nuclear pore complex required forpolyadenylated RNA export but not for proteinimport, homologous to S. pombe Rae1p 7e-050.00128 S000003657RPE1YJL121CverifiedD-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in thenon-oxidative part of the pentose-phosphatepathway; mutants are sensitive to oxidativestress 7e-050.00128 S000000467SHM1YBR263WverifiedMitochondrial serine hydroxymethyltransferase, involved inone-carbon metabolism 7e-050.00128 S000001678MIA40YKL195WverifiedEssential protein of the mitochondrial intermembrane space(IMS); promotes retention of newly importedproteins; may do so by stabilizing clientprotein folding as part of a disulfide relaysystem or transferring metal to client proteins 7e-050.00128 S000004826CEF1YMR213WverifiedEssential splicing factor; associated with Prp19p and thespliceosome, contains an N-terminal c-Myb DNAbinding motif necessary for cell viability butnot for Prp19p association, evolutionarilyconserved and homologous to S. pombe Cdc5p 7e-050.00128&radic S000004879RSN1YMR266WverifiedMembrane protein of unknown function; overexpression suppressesNaCl sensitivity of sro7 mutant 7e-050.00128 S000004212UTP13YLR222CverifiedNucleolar protein, component of the small subunit (SSU)processome containing the U3 snoRNA that isinvolved in processing of pre-18S rRNA 7e-050.00128 S000003706ASG7YJL170CverifiedProtein that regulates signaling from a G protein beta subunitSte4p and its relocalization within the cell;specific to a-cells and induced by alpha-factor 7e-050.00128 S000001212DBP8YHR169WverifiedPutative ATP-dependent RNA helicase of the DEAD-box familyinvolved in biogenesis of the 40S ribosomalsubunit 7e-050.00128 S000001018YHL026CUncharacterizedPutative protein of unknown function; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) 7e-050.00128 S000006397HPA2YPR193CverifiedTetrameric histone acetyltransferase with similarity to Gcn5p,Hat1p, Elp3p, and Hpa3p; acetylates histones H3and H4 in vitro and exhibits autoacetylationactivity 7e-050.00128 S000003193VRG4YGL225WverifiedGolgi GDP-mannose transporter; regulates Golgi function andglycosylation in Golgi 7e-050.00128&radic S000003407ERG1YGR175CverifiedSqualene epoxidase, catalyzes the epoxidation of squalene to2,3-oxidosqualene; plays an essential role inthe ergosterol-biosynthesis pathway and is thespecific target of the antifungal drugterbinafine 7e-050.00128&radic S000000126PET9YBL030CverifiedMajor ADP/ATP carrier of the mitochondrial inner membrane,exchanges cytosolic ADP for mitochondriallysynthesized ATP; required for viability in manycommon lab strains carrying a mutation in thepolymorphic SAL1 gene 7e-050.00128 S000001586LAP4YKL103CverifiedVacuolar aminopeptidase, often used as a marker protein instudies of autophagy and cytosol to vacuoletargeting (CVT) pathway 7e-050.00128 S000006315DBF20YPR111WverifiedSer/Thr kinase involved in late nuclear division, one of themitotic exit network (MEN) proteins; necessaryfor the execution of cytokinesis 7e-050.00128 S000000023MAK16YAL025CverifiedEssential nuclear protein, constituent of 66S pre-ribosomalparticles; required for maturation of 25S and5.8S rRNAs; required for maintenance of M1satellite double-stranded RNA of the L-A virus 7e-050.00128 S000004086KIN2YLR096WverifiedSerine/threonine protein kinase involved in regulation ofexocytosis; localizes to the cytoplasmic faceof the plasma membrane; closely related toKin1p 7e-050.00128 S000001883HXT10YFL011WverifiedPutative hexose transporter, expressed at low levels andexpression is repressed by glucose 7e-050.00128 S000000522NFS1YCL017CverifiedCysteine desulfurase involved in iron-sulfur cluster (Fe/S)biogenesis; required for thepost-transcriptional thio-modification ofmitochondrial and cytoplasmic tRNAs; essentialprotein located predominantly in mitochondria 7e-050.00128 S000005376CMK2YOL016CverifiedCalmodulin-dependent protein kinase; may play a role in stressresponse, many CA++/calmodulan dependentphosphorylation substrates demonstrated invitro, amino acid sequence similar to Cmk1p andmammalian Cam Kinase II 7e-050.00128 S000001797TGL4YKR089CverifiedTriacylglycerol lipase involved in triacylglycerol mobilizationand degradation; found in lipid particles;potential Cdc28p substrate 7e-050.00128 S000001128NAM8YHR086WverifiedRNA binding protein, component of the U1 snRNP protein; mutantsare defective in meiotic recombination and information of viable spores, involved in theformation of DSBs through meiosis-specificsplicing of MER2 pre-mRNA 7e-050.00128 S000003452MRPL9YGR220CverifiedMitochondrial ribosomal protein of the large subunit 7e-050.00128 S000002189DBP10YDL031WverifiedPutative ATP-dependent RNA helicase of the DEAD-box proteinfamily, constituent of 66S pre-ribosomalparticles; essential protein involved inribosome biogenesis 7e-050.00128 S000002300BPL1YDL141WverifiedBiotin:apoprotein ligase, covalently modifies proteins with theaddition of biotin, required for acetyl-CoAcarboxylase (Acc1p) holoenzyme formation 7e-050.00128 S000005432EMI5YOL071WverifiedNon-essential protein of unknown function required fortranscriptional induction of the earlymeiotic-specific transcription factor IME1,also required for sporulation 7e-050.00128 S000004490TSA1YML028WverifiedUbiquitous housekeeping thioredoxin peroxidase, reducesreactive oxygen, nitrogen and sulfur speciesusing thioredoxin as hydrogen donor; mediatesredox regulation of the nuclear localization ofYap1p; deletion results in mutator phenotype 7e-050.00128 S000006349ASN1YPR145WverifiedAsparagine synthetase, isozyme of Asn2p; catalyzes thesynthesis of L-asparagine from L-aspartate inthe asparagine biosynthetic pathway 7e-050.00128 S000002848DOT1YDR440WverifiedNucleosomal histone H3-Lys79 methylase, associates withtranscriptionally active genes, functions ingene silencing at telomeres, most likely bydirectly modulating chromatin structure and Sirprotein localization 7e-050.00128 S000005993UBP16YPL072WverifiedDeubiquitinating enzyme anchored to the outer mitochondrialmembrane, probably not important for generalmitochondrial functioning, but may perform amore specialized function at mitochondria 7e-050.00128 S000002927FPR2YDR519WverifiedMembrane-bound peptidyl-prolyl cis-trans isomerase (PPIase),binds to the drugs FK506 and rapamycin;expression pattern suggests possibleinvolvement in ER protein trafficking 7e-050.00128 S000028811YOL013W-AUncharacterizedIdentified by SAGE 7e-050.00128 S000000400PGI1YBR196CverifiedGlycolytic enzyme phosphoglucose isomerase, catalyzes theinterconversion of glucose-6-phosphate andfructose-6-phosphate; required for cell cycleprogression and completion of the gluconeogenicevents of sporulation 7e-050.00128&radic S000003628HPR5YJL092WverifiedDNA helicase and DNA-dependent ATPase involved in DNA repair,required for proper timing of commitment tomeiotic recombination and the transition fromMeiosis I to Meiosis II; potential Cdc28psubstrate 7e-050.00128 S000006208YPR004CVerifiedElectron transfer flavoprotein complex subunit ETF-alpha; contains a FAD binding domain; interacts with YFH1, the yeast frataxin homolog 7e-050.00128 S000004580CTK3YML112WverifiedGamma subunit of C-terminal domain kinase I (CTDK-I), whichphosphorylates the C-terminal repeated domainof the RNA polymerase II large subunit (Rpo21p)to affect both transcription and pre-mRNA 3'end processing 7e-050.00128 S000004085IOC2YLR095CverifiedMember of a complex (Isw1b) with Isw1p and Ioc4p that exhibitsnucleosome-stimulated ATPase activity and actswithin coding regions to coordinatetranscription elongation with termination andprocessing, contains a PHD finger motif 7e-050.00128 S000002370YDL211CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 7e-050.00128 S000002515GSG1YDR108WverifiedSubunit of TRAPP (transport protein particle), a multi-subunitcomplex involved in targeting and/or fusion ofER-to-Golgi transport vesicles with theiracceptor compartment; protein has late meioticrole, following DNA replication 7e-050.00128 S000006147NEW1YPL226WverifiedATP binding cassette family member; Asn/Gln-rich rich regionsupports [NU+] prion formation, susceptibilityto [PSI+] prion induction and aggregation of afragment of the human Machado-Joseph Diseaseprotein 7e-050.00128 S000003326VAS1YGR094WverifiedMitochondrial and cytoplasmic valyl-tRNA synthetase 7e-050.00128 S000003343YGR111WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 7e-050.00128 S000004307NKP2YLR315WverifiedNon-essential kinetochore protein, subunit of the Ctf19 centralkinetochore complex(Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-Nkp1p-Nkp2p-Ame1p-Mtw1p) 7e-050.00128 S000000285SPT7YBR081CverifiedSubunit of the SAGA transcriptional regulatory complex,involved in proper assembly of the complex;also present as a C-terminally truncated formin the SLIK/SALSA transcriptional regulatorycomplex 7e-050.00128&radic S000004954IDP3YNL009WverifiedPeroxisomal NADP-dependent isocitrate dehydrogenase, catalyzesoxidation of isocitrate to alpha-ketoglutaratewith the formation of NADP(H+), required forgrowth on unsaturated fatty acids 7e-050.00128 S000003234SWC4YGR002CverifiedProtein of unknown function, component of the Swr1p complexthat incorporates Htz1p into chromatin;component of the NuA4 histone acetyltransferasecomplex 7e-050.00128 S000004319TMA10YLR327CverifiedProtein of unknown function that associates with ribosomes 7e-050.00128 S000000835ZRG8YER033CverifiedProtein of unknown function; authentic, non-tagged protein isdetected in highly purified mitochondria inhigh-throughput studies; GFP-fusion protein islocalized to the cytoplasm; transcriptioninduced under conditions of zinc deficiency 7e-050.00128 S000000365CSH1YBR161WverifiedProbable catalytic subunit of a mannosylinositolphosphorylceramide (MIPC) synthase, forms acomplex with probable regulatory subunit Csg2p;function in sphingolipid biosynthesis isoverlapping with that of Sur1p 7e-050.00128 S000004203CRR1YLR213CverifiedPutative glycoside hydrolase of the spore wall envelope;required for normal spore wall assembly,possibly for cross-linking between the glucanand chitosan layers; expressed duringsporulation 7e-050.00128 S000003000AGA2YGL032CverifiedAdhesion subunit of a-agglutinin of a-cells, C-terminalsequence acts as a ligand for alpha-agglutinin(Sag1p) during agglutination, modified withO-linked oligomannosyl chains, linked toanchorage subunit Aga1p via two disulfide bonds 7e-050.00128 S000004481OST6YML019WverifiedSubunit of the oligosaccharyltransferase complex of the ERlumen, which catalyzes asparagine-linkedglycosylation of newly synthesized proteins;similar to and partially functionally redundantwith Ost3p 7e-050.00128 S000002954SOM1YEL059C-AverifiedSubunit of the mitochondrial inner membrane peptidase, which isrequired for maturation of mitochondrialproteins of the intermembrane space; Som1pfacilitates cleavage of a subset of substrates 7e-050.00128 S000005357AIF1YNR074CverifiedMitochondrial cell death effector that translocates to thenucleus in response to apoptotic stimuli,homolog of mammalian Apoptosis-Inducing Factor,putative reductase 7e-050.00128 S000000775PAU2YEL049WverifiedPart of 23-member seripauperin multigene family encoded mainlyin subtelomeric regions, active duringalcoholic fermentation, regulated byanaerobiosis, negatively regulated by oxygen,repressed by heme 7e-050.00128 S000000737GLC3YEL011WverifiedGlycogen branching enzyme, involved in glycogen accumulation;green fluorescent protein (GFP)-fusion proteinlocalizes to the cytoplasm in a punctatepattern 7e-050.00128 S000001177YCK1YHR135CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck2p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck2p 7e-050.00128&radic S000003661GCD14YJL125CverifiedSubunit of tRNA (1-methyladenosine) methyltransferase, withGcd10p, required for the modification of theadenine at position 58 in tRNAs, especiallytRNAi-Met; first identified as a negativeregulator of GCN4 expression 7e-050.00128 S000002336YDL177CUncharacterizedHypothetical protein 7e-050.00128 S000001894SPB4YFL002CverifiedPutative ATP-dependent RNA helicase, nucleolar protein requiredfor synthesis of 60S ribosomal subunits at alate step in the pathway; sediments with 66Spre-ribosomes in sucrose gradients 7e-050.00128 S000002913PSP1YDR505CverifiedAsn and gln rich protein of unknown function; high-copysuppressor of POL1 (DNA polymerase alpha) andpartial suppressor of CDC2 (polymerase delta)and CDC6 (pre-RC loading factor) mutations;overexpression results in growth inhibition 7e-050.00128&radic S000000856GIP2YER054CverifiedPutative regulatory subunit of the protein phosphatase Glc7p,involved in glycogen metabolism; contains aconserved motif (GVNK motif) that is also foundin Gac1p, Pig1p, and Pig2p 7e-050.00128 S000003332MDR1YGR100WverifiedCytoplasmic GTPase-activating protein for Ypt/Rab transportGTPases Ypt6p, Ypt31p and Sec4p; involved inrecycling of internalized proteins andregulation of Golgi secretory function 7e-050.00128 S000003936PUF3YLL013CverifiedProtein that regulates degradation of specific mRNAs such asCOX17, binds almost exclusively to cytoplasmicmRNAs encoding mitochondrial proteins, memberof the PUF protein family that contains Pumiliohomology domains 7e-050.00128 S000003349YGR117CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 7e-050.00128 S000004095SEN2YLR105CverifiedSubunit of the tRNA splicing endonuclease, which is composed ofSen2p, Sen15p, Sen34p, and Sen54p; Sen2pcontains the active site for tRNA 5' splicesite cleavage and has similarity to Sen34p andto Archaeal tRNA splicing endonuclease 7e-050.00128 S000003696YJL160CUncharacterizedPutative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis 7e-050.00128 S000006021ATG21YPL100WverifiedPhosphatidylinositol 3,5-bisphosphate-binding protein requiredfor maturation of pro-aminopeptidase I,predicted to fold as a seven-bladedbeta-propeller; displays punctate cytoplasmiclocalization 7e-050.00128 S000004401UTP21YLR409CuncharacterizedPossible U3 snoRNP protein involved in maturation of pre-18SrRNA, based on computational analysis oflarge-scale protein-protein interaction data 7e-050.00128 S000000010CYS3YAL012WverifiedCystathionine gamma-lyase, catalyzes one of the two reactionsinvolved in the transsulfuration pathway thatyields cysteine from homocysteine with theintermediary formation of cystathionine 7e-050.00128 S000001329YIL067CUncharacterizedHypothetical protein 7e-050.00128 S000003031PUS2YGL063WverifiedPutative pseudouridine synthase 7e-050.00128 S000001089AAP1YHR047CverifiedArginine/alanine aminopeptidase, overproduction stimulatesglycogen accumulation 7e-050.00128 S000004638IMP2YMR035WverifiedCatalytic subunit of the mitochondrial inner membrane peptidasecomplex, required for maturation ofmitochondrial proteins of the intermembranespace; complex contains Imp1p and Imp2p (bothcatalytic subunits), and Som1p 7e-050.00128 S000001020WSC4YHL028WverifiedER membrane protein involved in the translocation of solublesecretory proteins and insertion of membraneproteins into the ER membrane; may also have arole in the stress response but has onlypartial functional overlap with WSC1-3 7e-050.00128 S000002694YDR286CUncharacterizedHypothetical protein 7e-050.00128 S000002906SEC20YDR498CverifiedMembrane glycoprotein v-SNARE involved in retrograde transportfrom the Golgi to the ER; required for N- andO-glycosylation in the Golgi but not in the ER;forms a complex with the cytosolic Tip20p 7e-050.00128 S000002970ERP6YGL002WverifiedProtein with similarity to Emp24p and Erv25p, member of the p24family involved in ER to Golgi transport; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 7e-050.00128 S000004335GAS2YLR343WuncharacterizedPutative 1,3-beta-glucanosyltransferase, has similarity toGas1p 7e-050.00128 S000001451SQT1YIR012WverifiedEssential protein involved in a late step of 60S ribosomalsubunit assembly or modification; containsmultiple WD repeats; interacts with Qsr1p in atwo-hybrid assay 7e-050.00128 S000003143SAE2YGL175CverifiedProtein with a role in accurate meiotic and mitoticdouble-strand break repair; phosphorylated inresponse to DNA damage and required for normalresistance to DNA-damaging agents 7e-050.00128 S000003934SOF1YLL011WverifiedEssential subunit of the U3 (box C+D) snRNP complex requiredfor 2' O-methylation of pre-RNA; has similarityto the beta subunit of trimeric G-proteins andthe splicing factor Prp4p 7e-050.00128 S000001692STE6YKL209CverifiedATP-binding cassette (ABC) transporter required for the exportof a-factor, catalyzes ATP hydrolysis coupledto a-factor transport, contains 12transmembrane domains and two ATP bindingdomains, expressed only in MATa cells 7e-050.00128 S000002776YPR1YDR368Wverified2-methylbutyraldehyde reductase, may be involved in isoleucinecatabolism 7e-050.00128 S000003874RSM7YJR113CverifiedMitochondrial ribosomal protein of the small subunit, hassimilarity to E. coli S7 ribosomal protein 7e-050.00128 S000028688YML100W-AUncharacterizedPutative protein of unknown function 7e-050.00128 S000028518YCL058W-AUncharacterizedProtein of unknown function; identified by homology to Ashbya gossypii 7e-050.00128 S000003455HSV2YGR223CverifiedPhosphatidylinositol 3,5-bisphosphate-binding protein,predicted to fold as a seven-bladedbeta-propeller; displays punctate cytoplasmiclocalization 7e-050.00128&radic S000000414ERV15YBR210WverifiedProtein of unknown function, has similarity to Erv14p 7e-050.00128 S000001055ARD1YHR013CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 7e-050.00128 S000002960YER093C-AUncharacterizedHypothetical protein 7e-050.00128 S000000964RAD4YER162CverifiedProtein that recognizes and binds damaged DNA (with Rad23p)during nucleotide excision repair; subunit ofNuclear Excision Repair Factor 2 (NEF2);homolog of human XPC protein 7e-050.00128 S000002242SUB2YDL084WverifiedComponent of the TREX complex required for nuclear mRNA export;member of the DEAD-box RNA helicase superfamilyand is involved in early and late steps ofspliceosome assembly; homolog of the humansplicing factor hUAP56 7e-050.00128&radic S000005594VAM10YOR068CverifiedProtein involved in vacuole morphogenesis; acts at an earlystep of homotypic vacuole fusion that isrequired for vacuole tethering 7e-050.00128 S000004689YMR084WUncharacterizedHypothetical protein 7e-050.00128 S000004351ADE13YLR359WverifiedAdenylosuccinate lyase, catalyzes two steps in the 'de novo'purine nucleotide biosynthetic pathway 7e-050.00128 S000005190VPS75YNL246WverifiedProtein of unknown function involved in vacuolar proteinsorting; detected in the nucleus 7e-050.00128 S000001919PES4YFR023WverifiedPoly(A) binding protein, suppressor of DNA polymerase epsilonmutation, similar to Mip6p 7e-050.00128 S000004924GAS1YMR307WverifiedBeta-1.3-glucanosyltransferase, required for cell wallassembly; localizes to the cell surface via aglycosylphosphatidylinositol (GPI) anchor 7e-050.00128 S000005102PGA1YNL158WverifiedEssential protein required for maturation of Gas1p and Pho8p;green fluorescent protein (GFP)-fusion proteinlocalizes to the nuclear periphery; hassynthetic genetic interations with secretorypathway genes 7e-050.00128 S000000146SEC17YBL050WverifiedPeripheral membrane protein required for vesicular transportbetween ER and Golgi and for the 'priming' stepin homotypic vacuole fusion, part of thecis-SNARE complex; has similarity to alpha-SNAP 7e-050.00128 S000004356YLR364WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR364W is not an essential gene 7e-050.00128 S000005580VHS3YOR054CverifiedFunctionally redundant (see also SIS2) inhibitory subunit ofPpz1p, a PP1c-related ser/thr proteinphosphatase Z isoform; synthetically lethalwith sis2; putative phosphopantothenoylcysteinedecarboxylase involved in coenzyme Abiosynthesis 7e-050.00128 S000002314CLB3YDL155WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the G2/M transition; may beinvolved in DNA replication and spindleassembly; accumulates during S phase and G2,then targeted for ubiquitin-mediateddegradation 7e-050.00128&radic S000002558CTH1YDR151CverifiedMember of the CCCH zinc finger family; has similarity tomammalian Tis11 protein, which activatestranscription and also has a role in mRNAdegradation; may function with Tis11p in ironhomeostasis 7e-050.00128 S000004617ERG5YMR015CverifiedC-22 sterol desaturase, a cytochrome P450 enzyme that catalyzesthe formation of the C-22(23) double bond inthe sterol side chain in ergosterolbiosynthesis; may be a target of azoleantifungal drugs 7e-050.00128 S000005012FKH2YNL068CverifiedForkhead family transcription factor with a major role in theexpression of G2/M phase genes; positivelyregulates transcriptional elongation; negativerole in chromatin silencing at HML and HMR;substrate of the Cdc28p/Clb5p kinase 7e-050.00128 S000003011DST1YGL043WverifiedGeneral transcription elongation factor TFIIS, enables RNApolymerase II to read through blocks toelongation by stimulating cleavage of nascenttranscripts stalled at transcription arrestsites 7e-050.00128 S000005669THI80YOR143CverifiedThiamine pyrophosphokinase, phosphorylates thiamine to producethe coenzyme thiamine pyrophosphate (thiaminediphosphate) 7e-050.00128 S000001298CST6YIL036WverifiedBasic leucine zipper (bZIP) transcription factor of theATF/CREB family, activates transcription ofgenes involved in utilization of non-optimalcarbon sources; involved in telomeremaintenance 7e-050.00128 S000005177BNI4YNL233WverifiedTargeting subunit for Glc7p protein phosphatase, localized tothe bud neck, required for localization ofchitin synthase III to the bud neck viainteraction with the chitin synthase IIIregulatory subunit Skt5p 7e-050.00128 S000002335YDL176WUncharacterizedHypothetical protein 7e-050.00128 S000028804YJL047C-AUncharacterized 7e-050.00128 S000001850OTU1YFL044CverifiedDeubiquitylation enzyme that binds to the chaperone-ATPaseCdc48p; may contribute to regulation of proteindegradation by deubiquitylating substrates thathave been ubiquitylated by Ufd2p; member of theOvarian Tumor (OTU) family 7e-050.00128 S000003004YGL036WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene 7e-050.00128 S000003259RPS25AYGR027CverifiedProtein component of the small (40S) ribosomal subunit; nearlyidentical to Rps25Bp and has similarity to ratS25 ribosomal protein 7e-050.00128 S000005847GNT1YOR320CverifiedN-acetylglucosaminyltransferase capable of modification ofN-linked glycans in the Golgi apparatus 7e-050.00128 S000003085YGL117WUncharacterizedPutative protein of unknown function 7e-050.00128&radic S000000662RAD18YCR066WverifiedProtein involved in postreplication repair; bindssingle-stranded DNA and has single-stranded DNAdependent ATPase activity; forms heterodimerwith Rad6p; contains RING-finger motif 7e-050.00128 S000005338HOL1YNR055CverifiedPutative ion transporter similar to the major facilitatorsuperfamily of transporters; mutations inmembrane-spanning domains permit nonselectivecation uptake 7e-050.00128 S000002949YDR541CUncharacterizedHypothetical protein 7e-050.00128 S000002512TMS1YDR105CverifiedVacuolar membrane protein of unknown function that is conservedin mammals; predicted to contain eleventransmembrane helices; interacts with Pdr5p, aprotein involved in multidrug resistance 7e-050.00128 S000003189NIF3YGL221CverifiedProtein of unknown function, similar to Listeria monocytogenesmajor sigma factor (rpoD gene product); theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 7e-050.00128 S000004229LIP2YLR239CverifiedLipoyl ligase, involved in the modification of mitochondrialenzymes by the attachment of lipoic acid groups 7e-050.00128 S000003579YJL043WUncharacterizedPutative protein of unknown function; YJL043W is a non-essential gene 7e-050.00127 S000002517FOB1YDR110WverifiedNucleolar protein required for DNA replication fork blockingand recombinational hotspot activities; bindsto the replication fork barrier site in therDNA region; related to retroviral integrases 7e-050.00127 S000003950ISA1YLL027WverifiedMitochondrial matrix protein involved in biogenesis of theiron-sulfur (Fe/S) cluster of Fe/S proteins,isa1 deletion causes loss of mitochondrial DNAand respiratory deficiency; depletion reducesgrowth on nonfermentable carbon sources 7e-050.00127 S000003065SRM1YGL097WverifiedNucleotide exchange factor for Gsp1p, localizes to the nucleus,required for nucleocytoplasmic trafficking ofmacromolecules; potentially phosphorylated byCdc28p 7e-050.00127&radic S000000819AFG3YER017CverifiedComponent, with Yta12p, of the mitochondrial inner membranem-AAA protease that mediates degradation ofmisfolded or unassembled proteins and is alsorequired for correct assembly of mitochondrialenzyme complexes 7e-050.00127 S000005709FYV12YOR183WuncharacterizedProtein of unknown function, required for survival uponexposure to K1 killer toxin 7e-050.00127&radic S000003486ENO1YGR254WverifiedEnolase I, a phosphopyruvate hydratase that catalyzes theconversion of 2-phosphoglycerate tophosphoenolpyruvate during glycolysis and thereverse reaction during gluconeogenesis;expression is repressed in response to glucose 7e-050.00127 S000000007SPO7YAL009WverifiedIntegral nuclear/ER membrane protein of unknown function,required for normal nuclear envelope morphologyand sporulation 7e-050.00127 S000005271EGT2YNL327WverifiedGlycosylphosphatidylinositol (GPI)-anchored cell wallendoglucanase required for proper cellseparation after cytokinesis, expression isactivated by Swi5p and tightly regulated in acell cycle-dependent manner 7e-050.00127 S000002783BCS1YDR375CverifiedProtein of the mitochondrial inner membrane that functions asan ATP-dependent chaperone, required for theassembly of the cytochrome bc(1) complex fromthe Rip1p and Qcr10p proteins; member of theCDC48/PAS1/SEC18 ATPase family 7e-050.00127 S000002262QRI7YDL104CuncharacterizedPutative metalloprotease, similar to O-sialoglycoproteinmetallopeptidase from P. haemolytica; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 7e-050.00127 S000005796VPH1YOR270CverifiedSubunit of vacuolar-ATPase V0 domain, one of two isoforms(Vph1p and Stv1p); Vph1p is located in V-ATPasecomplexes of the vacuole while Stv1p is locatedin V-ATPase complexes of the Golgi andendosomes 7e-050.00127 S000006051SPO19YPL130WverifiedMeiosis-specific protein of unknown function, involved incompletion of nuclear divisions; identified asa weak high-copy suppressor of the spo1-1 tsmutation; putative GPI-dependent cell-wallprotein 7e-050.00127 S000000224GAL1YBR020WverifiedGalactokinase, phosphorylates alpha-D-galactose toalpha-D-galactose-1-phosphate in the first stepof galactose catabolism; expression regulatedby Gal4p 7e-050.00127 S000004011IRC25YLR021WuncharacterizedPutative protein of unknown function; mutant accumulatesunusually high amounts of dityrosine in thesoluble fraction though spores appearwild-type; YLR021W is not an essential gene;null mutant displays increased levels ofspontaneous Rad52 foci 7e-050.00127 S000005832YOR305WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; YOR305W is not an essential gene 7e-050.00127 S000004081YLR091WUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR091W is not an esssential gene 7e-050.00127 S000003581YJL045WUncharacterizedMinor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner 7e-050.00127 S000004815ERG2YMR202WverifiedC-8 sterol isomerase, catalyzes the isomerization of thedelta-8 double bond to the delta-7 position atan intermediate step in ergosterol biosynthesis 7e-050.00127 S000005992YPL071CUncharacterizedHypothetical protein 7e-050.00127 S000000949SCC4YER147CverifiedSubunit of cohesin loading factor (Scc2p-Scc4p), a complexrequired for the loading of cohesin complexesonto chromosomes; involved in establishingsister chromatid cohesion during double-strandbreak repair via phosphorylated histone H2AX 7e-050.00127 S000001156BZZ1YHR114WverifiedSH3 domain protein implicated in the regulation of actinpolymerization, able to recruit actinpolymerization machinery through its SH3domains, colocalizes with cortical actinpatches and Las17p, interacts with type Imyosins 7e-050.00127 S000001659LST4YKL176CverifiedProtein possibly involved in a post-Golgi secretory pathway;required for the transport ofnitrogen-regulated amino acid permease Gap1pfrom the Golgi to the cell surface 7e-050.00127 S000005785RPT4YOR259CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for spindlepole body duplication; localized mainly to thenucleus throughout the cell cycle 7e-050.00127 S000000138FUI1YBL042CverifiedHigh affinity uridine permease, localized to the plasmamembrane; not involved in uracil transport 7e-050.00127&radic S000001005OTU2YHL013CverifiedProtein of unknown function that may interact with ribosomes,based on co-purification experiments; member ofthe ovarian tumor-like (OTU) superfamily ofpredicted cysteine proteases; shows cytoplasmiclocalization 7e-050.00127 S000000349ADH5YBR145WverifiedAlcohol dehydrogenase isoenzyme V; involved in ethanolproduction 7e-050.00127 S000005215BNI1YNL271CverifiedFormin, nucleates the formation of linear actin filaments,involved in cell processes such as budding andmitotic spindle orientation which require theformation of polarized actin cables,functionally redundant with BNR1 7e-050.00127 S000005828YOR302WVerifiedCPA1 uORF , Arginine attenuator peptide, regulates translation of the CPA1 mRNA 7e-050.00127&radic S000028630YFR032C-BUncharacterizedPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 7e-050.00127 S000002622AHA1YDR214WverifiedCo-chaperone that binds to Hsp82p and activates its ATPaseactivity; similar to Hch1p; expression isregulated by stresses such as heat shock 7e-050.00127 S000003583RTT101YJL047CverifiedCullin subunit of a Roc1p-dependent E3 ubiquitin ligasecomplex; deletion phenotype suggests a role inanaphase progression; interacts with Mms22p andimplicated in Mms22-dependent DNA repair;modified by the ubiquitin-like protein, Rub1p 7e-050.00127 S000001569SRX1YKL086WverifiedSulfiredoxin, contributes to oxidative stress resistance byreducing cysteine-sulfinic acid groups in theperoxiredoxins Tsa1p and Ahp1p that are formedupon exposure to oxidants; conserved in highereukaryotes 7e-050.00127 S000002655VHS1YDR247WverifiedCytoplasmic serine/threonine protein kinase; identified as ahigh-copy suppressor of the synthetic lethalityof a sis2 sit4 double mutant, suggesting a rolein G1/S phase progression; homolog of Sks1p 7e-050.00127 S000003909BAT2YJR148WverifiedCytosolic branched-chain amino acid aminotransferase, homologof murine ECA39; highly expressed duringstationary phase and repressed duringlogarithmic phase 7e-050.00127 S000001762DYN1YKR054CverifiedCytoplasmic heavy chain dynein, microtubule motor protein,required for anaphase spindle elongation;involved in spindle assembly, chromosomemovement, and spindle orientation during celldivision, targeted to microtubule tips by Pac1p 7e-050.00127 S000000764UTR4YEL038WverifiedProtein of unknown function, found in both the cytoplasm andnucleus 7e-050.00127 S000002332YDL173WUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDL173W is not an essential gene 7e-050.00127 S000004765TPP1YMR156CverifiedDNA 3'-phosphatase that functions in repair of endogenousdamage of double-stranded DNA, activity isspecific for removal of 3' phosphates at strandbreaks; has similarity to the l-2-haloaciddehalogenase superfamily 7e-050.00127 S000028565YLR157C-CUncharacterizedPutative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-4 7e-050.00127 S000001120YHR078WUncharacterizedHypothetical protein 7e-050.00127 S000005635INP53YOR109WverifiedPhosphatidylinositol 4,5-bisphosphate 5-phosphatase,synaptojanin-like protein with an N-terminalSac1 domain, plays a role in a TGN (trans Golginetwork)-to-early endosome pathway;hyperosmotic stress causes translocation toactin patches 7e-050.00127 S000004696YMR090WUncharacterizedPutative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR090W is not an essential gene