Yfunc lets you browse current and predicted gene functions in yeast

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Prediction of GO:0043632 "modification-dependent macromolecule catabolism"

GO branch: BP
Number of currently annotated genes: 148

* the highlighted genes are those "interesting" predictions that
(1) are not currently annotated to this function,
(2) are not annotated with any ancestor GO terms (except the root term),
(3) have a projected precision score > 0.5
Histogram of scores for current annotations (annotated with the target function),
and novel predictions (not annotated with the target function)
Histogram of scores for current annotations, and predictions
SGDIDcommon namesystematic namefeature typeSGD descriptionCYGD reportannotation statusprediction scoreprojected precisionTop 10 important variables from random forest Harbison:RPN4_H2O2LoLum:CaffeineLum:IsoniazidKumar:cyto.Lum:TrifluoperazineLum:Actinomycin DDudley:HygroBLum:AsulamHuh:nucleusHuh:cytoplasm S000005785RPT4YOR259CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for spindlepole body duplication; localized mainly to thenucleus throughout the cell cycle &radic0.921831&radic&radic&radic&radic&radic&radic&radic S000001628RPT1YKL145WverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for optimalCDC20 transcription; interacts with Rpn12p andthe E3 ubiquitin-protein ligase Ubr1p &radic0.905731&radic&radic&radic&radic&radic&radic&radic S000005643RPT5YOR117WverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; recruited to theGAL1-10 promoter region upon induction oftranscription &radic0.897951&radic&radic&radic&radic&radic&radic S000003016RPT6YGL048CverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; bound byubiquitin-protein ligases Ubr1p and Ufd4p;localized mainly to the nucleus throughout thecell cycle &radic0.91521&radic&radic&radic&radic&radic&radic&radic S000006307PRE2YPR103Wverified20S proteasome beta-type subunit, responsible for thechymotryptic activity of the proteasome &radic0.926821&radic&radic S000000814PRE1YER012Wverified20S proteasome beta-type subunit; localizes to the nucleusthroughout the cell cycle &radic0.935911&radic&radic&radic&radic S000005398PRE6YOL038Wverified20S proteasome alpha-type subunit; green fluorescent protein(GFP)-fusion protein relocates from the cytosolto the mitochondrial outer surface uponoxidative stress &radic0.888490.98757&radic&radic&radic&radic&radic&radic S000002802RPT3YDR394WverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; substrate ofN-acetyltransferase B &radic0.88820.98757&radic&radic&radic&radic&radic&radic&radic S000002255RPN6YDL097CverifiedEssential, non-ATPase regulatory subunit of the 26S proteasomelid required for the assembly and activity ofthe 26S proteasome; the human homolog (S9protein) partially rescues Rpn6p depletion &radic0.873470.97695&radic&radic&radic&radic S000001243RPN10YHR200WverifiedNon-ATPase base subunit of the 19S regulatory particle (RP) ofthe 26S proteasome; N-terminus plays a role inmaintaining the structural integrity of the RP;binds selectively to polyubiquitin chains;homolog of the mammalian S5a protein &radic0.87030.97695&radic&radic&radic&radic&radic&radic S000000823RPN3YER021WverifiedEssential, non-ATPase regulatory subunit of the 26S proteasomelid, similar to the p58 subunit of the human26S proteasome; temperature-sensitive allelescause metaphase arrest, suggesting a role forthe proteasome in cell cycle control &radic0.870310.97695&radic&radic&radic&radic&radic&radic&radic&radic S000004557PRE8YML092Cverified20S proteasome beta-type subunit &radic0.859390.97058&radic&radic S000001946PRE4YFR050Cverified20S proteasome beta-type subunit &radic0.857130.9661&radic&radic&radic&radic&radic&radic&radic&radic&radic S000000763RAD23YEL037CverifiedProtein with ubiquitin-like N terminus, recognizes and bindsdamaged DNA (with Rad4p) during nucleotideexcision repair; regulates Rad4p levels,subunit of Nuclear Excision Repair Factor 2(NEF2); homolog of human HR23A and HR23Bproteins &radic0.847530.96242&radic&radic&radic&radic&radic&radic S000002835RPN9YDR427WverifiedNon-ATPase regulatory subunit of the 26S proteasome, hassimilarity to putative proteasomal subunits inother species; null mutant is temperaturesensitive and exhibits cell cycle andproteasome assembly defects &radic0.850360.96242&radic&radic S000001948RPN12YFR052WverifiedSubunit of the 19S regulatory particle of the 26S proteasomelid; synthetically lethal with RPT1, which isan ATPase component of the 19S regulatoryparticle; physically interacts with Nob1p andRpn3p &radic0.839560.95833&radic&radic&radic&radic&radic&radic S000003538PRE3YJL001Wverified20S proteasome beta-type subunit, responsible for cleavageafter acidic residues in peptides &radic0.827760.95833&radic&radic S000006312RPN7YPR108WverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome,similar to another S. cerevisiae regulatorysubunit, Rpn5p, as well as to mammalianproteasome subunits &radic0.79830.95833&radic&radic&radic&radic&radic&radic&radic&radic S000002290CDC53YDL132WverifiedCullin, structural protein of SCF complexes (which also containSkp1p, Cdc34p, and an F-box protein) involvedin ubiquitination; SCF promotes the G1-Stransition by targeting G1 cyclins and theCln-CDK inhibitor Sic1p for degradation &radic0.795930.95833&radic&radic&radic S000005787RPN8YOR261CverifiedEssential, non-ATPase regulatory subunit of the 26S proteasome;has similarity to the human p40 proteasomalsubunit and to another S. cerevisiae regulatorysubunit, Rpn11p &radic0.829850.95833&radic&radic&radic&radic&radic&radic&radic&radic&radic S000002979SCL1YGL011CverifiedAlpha subunit of the 20S core complex of the 26S proteasomeinvolved in the degradation of ubiquitinatedsubstrates; essential for growth; detected inthe mitochondria &radic0.793130.95833&radic&radic&radic&radic&radic S000003367PRE9YGR135Wverified20S proteasome beta-type subunit; the only nonessential 20Ssubunit &radic0.788760.95833&radic&radic&radic&radic&radic&radic&radic&radic S000004117APC2YLR127CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; similar tocullin Cdc53p &radic0.836440.95833&radic&radic&radic&radic&radic&radic&radic S000005116APC1YNL172WverifiedLargest subunit of the Anaphase-Promoting Complex/Cyclosome(APC/C), which is a ubiquitin-protein ligaserequired for degradation of anaphaseinhibitors, including mitotic cyclins, duringthe metaphase/anaphase transition &radic0.794660.95833&radic&radic&radic&radic&radic&radic S000001900RPN11YFR004WverifiedMetalloprotease subunit of the 19S regulatory particle of the26S proteasome lid; couples thedeubiquitination and degradation of proteasomesubstrates &radic0.838340.95833&radic&radic&radic&radic&radic&radic&radic S000003485PUP2YGR253CverifiedAlpha subunit of the 20S proteasome involved inubiquitin-dependent catabolism; human homologis subunit zeta &radic0.783150.95652&radic&radic&radic&radic&radic S000000896PUP3YER094CverifiedBeta subunit of the 20S proteasome involved inubiquitin-dependent catabolism; human homologis subunit C10 &radic0.775130.95638&radic S000005889PRE10YOR362Cverified20S proteasome alpha-type subunit &radic0.774570.95638&radic&radic S000001069RPN1YHR027CverifiedNon-ATPase base subunit of the 19S regulatory particle of the26S proteasome; may participate in therecognition of several ligands of theproteasome; contains a leucine-rich repeat(LRR) domain, a site for protein?proteininteractions &radic0.768980.95069&radic&radic&radic&radic S000002306RPN5YDL147WverifiedEssential, non-ATPase regulatory subunit of the 26S proteasomelid, similar to mammalian p55 subunit and toanother S. cerevisiae regulatory subunit, Rpn7p &radic0.766290.94898&radic&radic S000004931PRE5YMR314Wverified20S proteasome alpha-type subunit &radic0.765280.9485&radic&radic&radic S000005683PUP1YOR157CverifiedEndopeptidase with trypsin-like activity that cleaves afterbasic residues; beta-type subunit of 20Sproteasome synthesized as a proprotein beforebeing proteolytically processed for assemblyinto 20S particle; human homolog is subunit Z &radic0.753130.93975&radic&radic&radic&radic&radic&radic&radic&radic S000001505CDC16YKL022CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition; required forsporulation &radic0.741010.93868&radic&radic&radic&radic&radic S000002165RPT2YDL007WverifiedOne of six ATPases of the 19S regulatory particle of the 26Sproteasome involved in the degradation ofubiquitinated substrates; required for normalpeptide hydrolysis by the core 20S particle &radic0.736670.93489&radic S000001337RPN2YIL075CverifiedSubunit of the 26S proteasome, substrate of theN-acetyltransferase Nat1p &radic0.728960.93455&radic&radic&radic&radic S000001209CDC23YHR166CverifiedSubunit of the anaphase-promoting complex/cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.724040.93455&radic S000004553UFO1YML088WverifiedF-box receptor protein, subunit of the Skp1-Cdc53-F-boxreceptor (SCF) E3 ubiquitin ligase complex;binds to phosphorylated Ho endonuclease,allowing its ubiquitylation by SCF andsubsequent degradation &radic0.7220.93455&radic S000000180CDC27YBL084CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.72560.93455&radic&radic&radic S000003209DOC1YGL240WverifiedProcessivity factor required for the ubiquitination activity ofthe anaphase promoting complex (APC), mediatesthe activity of the APC by contributing tosubstrate recognition; involved in cyclinproteolysis &radic0.702570.92372&radic&radic&radic&radic&radic S000005606DIA2YOR080WverifiedOrigin-binding F-box protein that forms an SCF ubiquitin ligasecomplex with Skp1p and Cdc53p; plays a role inDNA replication, involved in invasive andpseudohyphal growth 0.701470.92312&radic&radic&radic&radic S000001885CDC4YFL009WverifiedF-box protein required for G1/S and G2/M transition, associateswith Skp1p and Cdc53p to form a complex,SCFCdc4, which acts as ubiquitin-protein ligasedirecting ubiquitination of the phosphorylatedCDK inhibitor Sic1p &radic0.696280.92161&radic S000003685YJL149WVerifiedPutative SCF ubiquitin ligase F-box protein of unknown function; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C; YJL149W is a non-essential gene 0.684390.91679&radic&radic&radic S000000137PRE7YBL041Wverified20S proteasome beta-type subunit &radic0.68240.91642&radic S000002736SKP1YDR328CverifiedEvolutionarily conserved kinetochore protein that is part ofmultiple protein complexes, including the SCFubiquitin ligase complex, the CBF3 complex thatbinds centromeric DNA, and the RAVE complexthat regulates assembly of the V-ATPase &radic0.677030.91626&radic&radic&radic&radic&radic&radic S000003850GRR1YJR090CverifiedF-box protein component of the SCF ubiquitin-ligase complex,required for Cln1p and Cln2p degradation;involved in carbon catabolite repression,glucose-dependent divalent cation transport,high-affinity glucose transport, andmorphogenesis &radic0.657510.90865 S000002461CDC34YDR054CverifiedUbiquitin-conjugating enzyme or E2; together with Skp1p, Rbx1p,Cdc53p, and an F-box protein, forms aubiquitin-protein ligase called the SCF complexwhich regulates cell cycle progression bytargeting key substrates for degradation &radic0.648780.90008&radic&radic&radic&radic&radic S000005475PAP2YOL115WverifiedCatalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), anuclear poly (A) polymerase complex involved inRNA quality control; catalyzes polyadenylationof unmodified tRNAs, and snoRNA and rRNAprecursors; disputed role as a DNA polymerase &radic0.644610.89943&radic S000001308MET30YIL046WverifiedF-box protein containing five copies of the WD40 motif,controls cell cycle function, sulfurmetabolism, and methionine biosynthesis as partof the ubiquitin ligase complex; interacts withand regulates Met4p, localizes within thenucleus &radic0.632480.88876&radic&radic&radic&radic&radic&radic&radic S000003740RCY1YJL204CverifiedF-box protein involved in recycling plasma membrane proteinsinternalized by endocytosis; localized to sitesof polarized growth 0.608380.87776&radic&radic&radic S000004360MDM30YLR368WverifiedF-box protein; physically associates with mitochondria and isrequired for normal mitochondrial fusion inrich medium, during sporulation, and in matingcells; promotes ubiquitin-mediated degradationof Gal4p in some strains 0.598590.87167&radic&radic&radic&radic S000002738UBX5YDR330WuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p &radic0.59590.86929&radic&radic&radic&radic&radic&radic S000002466UBC5YDR059CverifiedUbiquitin-conjugating enzyme that mediates selectivedegradation of short-lived and abnormalproteins, central component of the cellularstress response; expression is heat inducible 0.596370.86929&radic&radic&radic&radic S000000154SHP1YBL058WverifiedUBX (ubiquitin regulatory X) domain-containing protein thatregulates Glc7p phosphatase activity andinteracts with Cdc48p; interacts withubiquitylated proteins in vivo and is requiredfor degradation of a ubiquitylated modelsubstrate &radic0.595250.86834&radic&radic&radic&radic&radic S000000286UBC4YBR082CverifiedUbiquitin-conjugating enzyme that mediates degradation ofshort-lived and abnormal proteins; interactswith E3-CaM in ubiquitinating calmodulin;interacts with many SCF ubiquitin proteinligases; component of the cellular stressresponse &radic0.567520.85332&radic&radic&radic S000003583RTT101YJL047CverifiedCullin subunit of a Roc1p-dependent E3 ubiquitin ligasecomplex; deletion phenotype suggests a role inanaphase progression; interacts with Mms22p andimplicated in Mms22-dependent DNA repair;modified by the ubiquitin-like protein, Rub1p &radic0.565310.85016&radic&radic S000004624QRI8YMR022WverifiedUbiquitin conjugating enzyme, involved in the ER-associatedprotein degradation pathway; requires Cue1p forrecruitment to the ER membrane; proposed to beinvolved in chromatin assembly &radic0.551840.83987&radic&radic S000004087HRT3YLR097CuncharacterizedPutative nuclear ubiquitin ligase, based on computationalanalysis of large-scale protein-proteininteraction data; has similarity to F-boxproteins; identified in association withCdc53p, Skp1p and Ubi4 in large-scale studies &radic0.549070.83926&radic&radic&radic&radic&radic S000002249UBX3YDL091CuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p, green fluorescentprotein (GFP)-fusion protein localizes to thecytoplasm in a punctate pattern 0.550040.83926&radic S000003026RAD6YGL058WverifiedUbiquitin-conjugating enzyme (E2), involved in postreplicationrepair (with Rad18p), sporulation, telomeresilencing, and ubiquitin-mediated N-end ruleprotein degradation (with Ubr1p) &radic0.548660.83851&radic&radic&radic&radic&radic&radic&radic&radic S000002538YDR131CUncharacterizedF-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex &radic0.547460.83794&radic&radic S000002584UBC1YDR177WverifiedUbiquitin-conjugating enzyme that mediates selectivedegradation of short-lived and abnormalproteins; plays a role in vesicle biogenesisand ER-associated protein degradation (ERAD);component of the cellular stress response &radic0.543430.83523&radic&radic&radic&radic&radic&radic&radic S000000477UBX7YBR273CuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p &radic0.534970.83293&radic S000004671UBX4YMR067CuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p &radic0.525190.82476&radic&radic&radic S000005255SKP2YNL311CverifiedPutative F-box protein; analysis of integrated high-throughputdatasets predicts an involvement inubiquitin-dependent protein catabolism &radic0.518790.82194&radic&radic&radic&radic S000004475SEL1YML013WverifiedProtein involved in ER-associated protein degradation; proposedto coordinate the assembly of proteins involvedin ERAD; contains a UBX (ubiquitin regulatoryX) domain and a ubiquitin-associated (UBA)domain &radic0.519480.82194&radic&radic&radic&radic&radic S000004015SNF7YLR025WverifiedOne of four subunits of the endosomal sorting complex requiredfor transport III (ESCRT-III); involved in thesorting of transmembrane proteins into themultivesicular body (MVB) pathway; recruitedfrom the cytoplasm to endosomal membranes &radic0.51280.82028&radic&radic&radic&radic&radic S000003584UBX6YJL048CuncharacterizedUBX (ubiquitin regulatory X) domain-containing protein thatinteracts with Cdc48p, transcription isrepressed when cells are grown in mediacontaining inositol and choline &radic0.511980.82028 S000001906UBP6YFR010WverifiedUbiquitin-specific protease situated in the base subcomplex ofthe 26S proteasome, releases free ubiquitinfrom branched polyubiquitin chains; deletioncauses hypersensitivity to cycloheximide andother toxic compounds 0.509420.81972&radic&radic&radic&radic&radic&radic S000003730CDC6YJL194WverifiedEssential ATP-binding protein required for DNA replication,component of the pre-replicative complex(pre-RC) which requires ORC to associate withchromatin and is in turn required for Mcm2-7pDNA association; homologous to S. pombe Cdc18p 0.481150.80211&radic&radic&radic&radic&radic S000000514STP22YCL008CverifiedComponent of the ESCRT-I complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; homologous to the mouse and humanTsg101 tumor susceptibility gene; mutantsexhibit a Class E Vps phenotype &radic0.477660.79918&radic&radic&radic&radic&radic&radic S000004889DSK2YMR276WverifiedNuclear-enriched ubiquitin-like polyubiquitin-binding protein,required for spindle pole body (SPB)duplication and for transit through the G2/Mphase of the cell cycle, involved inproteolysis, interacts with the proteasome &radic0.474670.79679&radic&radic&radic&radic&radic&radic&radic&radic&radic S000004413RPN13YLR421CverifiedSubunit of the 19S regulatory particle of the 26S proteasomelid 0.472080.79505&radic&radic&radic&radic&radic&radic S000005243TRF5YNL299WverifiedPoly (A) polymerase involved in nuclear RNA quality controlbased on: homology with Trf4p, geneticinteractions with TRF4 mutants, physicalinteraction with Mtr4p (TRAMP subunit), and bydirect assay; disputed role as a sigma DNApolymerase &radic0.464040.7918&radic&radic&radic S000002499UBC13YDR092WverifiedUbiquitin-conjugating enzyme involved in the error-free DNApostreplication repair pathway; interacts withMms2p to assemble ubiquitin chains at the UbLys-63 residue; DNA damage triggersredistribution from the cytoplasm to thenucleus 0.438060.77527&radic&radic S000001855ACT1YFL039CverifiedActin, structural protein involved in cell polarization,endocytosis, and other cytoskeletal functions 0.435230.77201&radic&radic&radic S000000484SAF1YBR280CverifiedF-Box protein involved in proteasome-dependent degradation ofAah1p during entry of cells into quiescence;interacts with Skp1 &radic0.431380.77034 S000003457AMA1YGR225WverifiedActivator of meiotic anaphase promoting complex (APC/C); Cdc20pfamily member; required for initiation of sporewall assembly; required for Clb1p degradationduring meiosis 0.418730.76295&radic&radic&radic&radic&radic S000001022ECM29YHL030WverifiedMajor component of the proteasome; tethers the proteasome coreparticle to the regulatory particle, andenhances the stability of the proteasome 0.413750.75881&radic&radic&radic&radic&radic&radic&radic S000002349UFD2YDL190CverifiedUbiquitin chain assembly factor (E4) that cooperates with aubiquitin-activating enzyme (E1), aubiquitin-conjugating enzyme (E2), and aubiquitin protein ligase (E3) to conjugateubiquitin to substrates; also functions as an &radic0.413010.75777&radic&radic S000002476DOA4YDR069CverifiedUbiquitin hydrolase, required for recycling ubiquitin fromproteasome-bound ubiquitinated intermediates,acts at the late endosome/prevacuolarcompartment to recover ubiquitin fromubiquitinated membrane proteins en route to thevacuole 0.410760.75563&radic&radic S000004682VPS20YMR077CverifiedMyristoylated subunit of ESCRTIII, the endosomal sortingcomplex required for transport of transmembraneproteins into the multivesicular body pathwayto the lysosomal/vacuolar lumen; cytoplasmicprotein recruited to endosomal membranes &radic0.41050.75559&radic&radic S000005493HRT1YOL133WverifiedRING finger containing subunit of Skp1-Cullin-F-box ubiquitinprotein ligases (SCF); required for Gic2p,Far1p, Sic1p and Cln2p degradation; may tetherCdc34p (a ubiquitin conjugating enzyme or E2)and Cdc53p (a cullin) subunits of SCF &radic0.404350.74871&radic S000001524VPS24YKL041WverifiedOne of four subunits of the endosomal sorting complex requiredfor transport III (ESCRT-III); forms anESCRT-III subcomplex with Did4p; involved inthe sorting of transmembrane proteins into themultivesicular body (MVB) pathway &radic0.402480.74705&radic&radic&radic&radic&radic S000002222UBC9YDL064WverifiedSUMO-conjugating enzyme involved in the Smt3p conjugationpathway; nuclear protein required for S- andM-phase cyclin degradation and mitotic control;involved in proteolysis mediated by theanaphase-promoting complex cyclosome (APCC) 0.399460.74483&radic&radic&radic&radic S000004952SIS1YNL007CverifiedType II HSP40 co-chaperone that interacts with the HSP70protein Ssa1p; not functionally redundant withYdj1p due to due to substrate specificity;shares similarity with bacterial DnaJ proteins 0.386520.73478&radic&radic S000002971CDH1YGL003CverifiedCell-cycle regulated activator of the anaphase-promotingcomplex/cyclosome (APC/C), which directsubiquitination of mitotic cyclins resulting inexit from mitosis; targets the APC/C tospecific substrates including CDC20, ASE1 andCIN8 &radic0.38320.73201&radic&radic&radic&radic S000004344YLR352WUncharacterizedPutative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene 0.369640.71893&radic&radic&radic&radic&radic S000005373HRD1YOL013CverifiedUbiquitin-protein ligase required for endoplasmicreticulum-associated degradation (ERAD) ofmisfolded proteins; genetically linked to theunfolded protein response (UPR); regulatedthrough association with Hrd3p; contains an H2ring finger &radic0.356490.70226&radic&radic&radic&radic S000005174ELA1YNL230CverifiedElongin A, F-box protein that forms a heterodimer with Elc1pand participates in transcription elongation 0.352490.69579&radic&radic&radic&radic&radic&radic&radic S000002714YDR306CUncharacterizedF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain &radic0.34950.69174&radic&radic&radic S000001341AIR1YIL079CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p to regulate methylationof Npl3p, which modulates Npl3p function inmRNA processing and export; has similarity toAir2p &radic0.345920.68722&radic&radic&radic&radic&radic S000001710PAP1YKR002WverifiedPoly(A) polymerase, one of three factors required for mRNA3'-end polyadenylation, forms multiproteincomplex with polyadenylation factor I (PF I),also required for mRNA nuclear export; may alsopolyadenylate rRNAs 0.345780.68712&radic&radic&radic&radic&radic S000004297UBC12YLR306WverifiedEnzyme that mediates the conjugation of Rub1p, a ubiquitin-likeprotein, to other proteins; related to E2ubiquitin-conjugating enzymes 0.343470.68497&radic&radic&radic S000003962UBI4YLL039CverifiedUbiquitin, becomes conjugated to proteins, marking them forselective degradation via the ubiquitin-26Sproteasome system; essential for the cellularstress response 0.342990.68463&radic&radic&radic&radic&radic S000001150GGA2YHR108WverifiedGolgi-localized protein with homology to gamma-adaptin,interacts with and regulates Arf1p and Arf2p ina GTP-dependent manner in order to facilitatetraffic through the late Golgi &radic0.342810.68462&radic&radic&radic&radic S000006377VPS4YPR173CverifiedAAA-type ATPase required for efficient late endosome to vacuoletransport; catalyzes the release of anendosomal membrane-associated class E VPSprotein complex; regulates cellular sterolmetabolism; cytoplasmic and endosomallocalization 0.340670.6823&radic&radic&radic&radic&radic S000003416UBR1YGR184CverifiedUbiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2pto ubiquitinate substrates of the N-end rulepathway; binds to the Rpn2p, Rpt1p, and Rpt6pproteins of the 19S particle of the 26Sproteasome 0.317090.65235&radic&radic S000003055MMS2YGL087CverifiedProtein involved in error-free postreplication DNA repair;forms a heteromeric complex with Ubc13p thathas a ubiquitin-conjugating activity;cooperates with chromatin-associated RINGfinger proteins, Rad18p and Rad5p 0.314180.64983&radic&radic&radic&radic&radic S000004025MLH2YLR035CverifiedProtein required for DNA mismatch repair in mitosis andmeiosis; involved in the repair of mutationalintermediates; forms a complex with Pms1p andMsh1p to repair mismatched DNA 0.313040.64752&radic&radic S000002766GGA1YDR358WverifiedGolgi-localized protein with homology to gamma-adaptin,interacts with and regulates Arf1p and Arf2p ina GTP-dependent manner in order to facilitatetraffic through the late Golgi &radic0.310290.64419&radic&radic&radic&radic&radic S000004827SCJ1YMR214WverifiedOne of several homologs of bacterial chaperone DnaJ, located inthe ER lumen where it cooperates with Kar2p tomediate maturation of proteins &radic0.309720.64326&radic&radic&radic&radic S000000284SEC18YBR080CverifiedATPase required for the release of Sec17p during the 'priming'step in homotypic vacuole fusion and for ER toGolgi transport; homolog of the mammalian NSF 0.304010.63747&radic S000003084CDC20YGL116WverifiedCell-cycle regulated activator of anaphase-promotingcomplex/cyclosome (APC/C), which is requiredfor metaphase/anaphase transition; directsubiquitination of mitotic cyclins, Pds1p, andother anaphase inhibitors; potential Cdc28psubstrate &radic0.304020.63747&radic&radic S000005986VPS28YPL065WverifiedComponent of the ESCRT-I complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; involved in transport ofprecursors for soluble vacuolar hydrolases fromthe late endosome to the vacuole &radic0.302770.6361&radic&radic&radic&radic&radic S000001887BLM10YFL007WverifiedProteosome activator subunit; found in association with coreparticles, with and without the 19S regulatoryparticle; required for resistance to bleomycin,may be involved in protecting against oxidativedamage; similar to mammalian PA200 0.300460.63306&radic&radic&radic&radic S000003464NAS6YGR232WverifiedRegulatory, non-ATPase subunit of the 26S proteasome; homologof the human oncoprotein gankyrin, whichinteracts with the retinoblastoma tumorsuppressor (Rb) and cyclin-dependent kinase 4/6 0.298950.63108&radic&radic&radic&radic&radic S000001696DOA1YKL213CverifiedWD repeat protein required for ubiquitin-mediated proteindegradation, forms complex with Cdc48p, plays arole in controlling cellular ubiquitinconcentration; also promotes efficient NHEJ inpostdiauxic/stationary phase &radic0.297930.62965&radic&radic&radic&radic&radic S000000902UBC6YER100WverifiedUbiquitin-conjugating enzyme involved in ER-associated proteindegradation; located at the cytosolic side ofthe ER membrane; tail region contains atransmembrane segment at the C-terminus;substrate of the ubiquitin-proteasome pathway &radic0.296890.62808 S000000945DDI1YER143WverifiedDNA damage-inducible v-SNARE binding protein, contains aubiquitin-associated (UBA) domain, may act as anegative regulator of constitutive exocytosis,may play a role in S-phase checkpoint control &radic0.294440.62629&radic&radic S000004409VPS36YLR417WverifiedComponent of the ESCRT-II complex; contains the GLUE (GRAM LikeUbiquitin binding in EAP45) domain which isinvolved in interactions with ESCRT-I andubiquitin-dependent sorting of proteins intothe endosome &radic0.279040.60849&radic&radic&radic&radic&radic&radic S000000318RAD16YBR114WverifiedProtein that recognizes and binds damaged DNA in anATP-dependent manner (with Rad7p) duringnucleotide excision repair; subunit ofNucleotide Excision Repair Factor 4 (NEF4);member of the SWI/SNF family 0.27620.6048&radic&radic S000001111RRP4YHR069CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp41p, Rrp42p,Rrp43p and Dis3p 0.274510.60305&radic&radic&radic&radic&radic S000003235CUL3YGR003WverifiedUbiquitin-protein ligase, member of the cullin family withsimilarity to Cdc53p and human CUL3; nullmutation has no apparent phenotype &radic0.274050.60251&radic&radic&radic&radic&radic S000005289VPS27YNR006WverifiedEndosomal protein that forms a complex with Hse1p; required forrecycling Golgi proteins, forming lumenalmembranes and sorting ubiquitinated proteinsdestined for degradation; has UbiquitinInteraction Motifs which bind ubiquitin (Ubi4p) 0.269510.59644&radic S000005553STI1YOR027WverifiedHsp90 cochaperone, interacts with the Ssa group of thecytosolic Hsp70 chaperones; activates theATPase activity of Ssa1p; homolog of mammalianHop protein 0.266710.59307&radic&radic&radic&radic&radic S000002627MFB1YDR219CverifiedMitochondria-associated F-box protein involved in maintenanceof normal mitochondrial morphology; interactswith Skp1p through the F-box motif;preferentially localizes to the mother cellduring budding &radic0.26040.58471&radic&radic&radic&radic&radic S000000374NPL4YBR170CverifiedEndoplasmic reticulum and nuclear membrane protein, forms acomplex with Cdc48p and Ufd1p that recognizesubiquitinated proteins in the endoplasmicreticulum and delivers them to the proteasomefor degradation &radic0.257970.58157&radic&radic S000005923SNF8YPL002CverifiedComponent of the ESCRT-II complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; appears to be functionallyrelated to SNF7; involved in glucosederepression &radic0.256990.58029&radic&radic&radic&radic S000001247MNL1YHR204WverifiedAlpha mannosidase-like protein of the endoplasmic reticulumrequired for degradation of glycoproteins butnot for processing of N-linked oligosaccharides &radic0.256280.57952&radic S000001556LHS1YKL073WverifiedMolecular chaperone of the endoplasmic reticulum lumen,involved in polypeptide translocation andfolding; member of the Hsp70 family; localizesto the lumen of the ER; regulated by theunfolded protein response pathway 0.255030.57712 S000001140SFB3YHR098CverifiedMember of the Sec24p family; forms a complex, with Sec23p, thatis involved in sorting of Pma1p into COPIIvesicles; peripheral ER membrane protein;potential Cdc28p substrate 0.246250.56477 S000006323CLB2YPR119WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the transition from G2 to Mphase; accumulates during G2 and M, thentargeted via a destruction box motif forubiquitin-mediated degradation by theproteasome 0.241850.55922&radic&radic&radic&radic&radic S000005391SIL1YOL031CverifiedNucleotide exchange factor for the endoplasmic reticulum (ER)lumenal Hsp70 chaperone Kar2p, required forprotein translocation into the ER; homolog ofYarrowia lipolytica SLS1; GrpE-like protein 0.231130.54514&radic&radic&radic S000000407COS111YBR203WverifiedProtein required for resistance to the antifungal drugciclopirox olamine; not related to thesubtelomerically-encoded COS family; theauthentic, non-tagged protein is detected inhighly purified mitochondria in high-throughputstudies 0.230240.54386&radic&radic&radic&radic&radic S000001371SEC24YIL109CverifiedComponent of the Sec23p-Sec24p heterodimeric complex of theCOPII vesicle coat; involved in ER to Golgitransport, cargo selection and autophagy;required for the binding of the Sec13 complexto ER membranes; homologous to Lst1p and Lss1p 0.227540.54018&radic S000004742GID8YMR135CverifiedProtein of unknown function, involved in proteasome-dependentcatabolite inactivation offructose-1,6-bisphosphatase; contains LisH andCTLH domains, like Vid30p; dosage-dependentregulator of START 0.226910.53911&radic&radic&radic S000002334AIR2YDL175CverifiedRING finger protein that interacts with the argininemethyltransferase Hmt1p; may regulatemethylation of Npl3p, which modulates Npl3pfunction in mRNA processing and export; hassimilarity to Air1p &radic0.22660.5386&radic&radic S000002166APC11YDL008WverifiedCatalytic core subunit of the Anaphase-PromotingComplex/Cyclosome (APC/C), which is aubiquitin-protein ligase required fordegradation of anaphase inhibitors, includingmitotic cyclins, during the metaphase/anaphasetransition &radic0.2230.53324 S000000605PGK1YCR012Wverified3-phosphoglycerate kinase, catalyzes transfer of high-energyphosphoryl groups from the acyl phosphate of1,3-bisphosphoglycerate to ADP to produce ATP;key enzyme in glycolysis and gluconeogenesis 0.220250.52931&radic&radic&radic&radic S000000662RAD18YCR066WverifiedProtein involved in postreplication repair; bindssingle-stranded DNA and has single-stranded DNAdependent ATPase activity; forms heterodimerwith Rad6p; contains RING-finger motif 0.216860.52397 S000002375RRI1YDL216CverifiedCatalytic subunit of the COP9 signalosome (CSN) complex thatacts as an isopeptidase in cleaving theubiquitin-like protein Nedd8 from SCF ubiquitinligases; metalloendopeptidase involved in theadaptation to pheromone signaling 0.213980.51935&radic S000005780SEC63YOR254CverifiedEssential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p andSec72p); with Sec61 complex, Kar2p/BiP andLhs1p forms a channel competent forSRP-dependent and post-translationalSRP-independent protein targeting and importinto the ER 0.21390.51907&radic&radic&radic S000004697NPL6YMR091CverifiedComponent of the RSC chromatin remodeling complex; interactswith Rsc3p, Rsc30p, Ldb7p, and Htl1p to form amodule important for a broad range of RSCfunctions; involved in nuclear protein importand maintenance of proper telomere length 0.211080.51463&radic S000002796RVS167YDR388WverifiedActin-associated protein, subunit of a complex(Rvs161p-Rvs167p) involved in regulation ofactin cytoskeleton, endocytosis, and viabilityfollowing starvation or osmotic stress; homologof mammalian amphiphysin 0.210060.51271&radic&radic&radic&radic S000004096MDN1YLR106CverifiedHuge dynein-related AAA-type ATPase (midasin), forms extendedpre-60S particle with the Rix1 complex(Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependentremodeling of 60S subunits and subsequentexport from nucleoplasm to cytoplasm 0.209170.51158&radic&radic&radic&radic S000003693FAR1YJL157CverifiedCyclin-dependent kinase inhibitor that mediates cell cyclearrest in response to pheromone; also forms acomplex with Cdc24p, Ste4p, and Ste18p that mayspecify the direction of polarized growthduring mating; potential Cdc28p substrate 0.20780.50865&radic&radic&radic S000002435REG1YDR028CverifiedRegulatory subunit of type 1 protein phosphatase Glc7p,involved in negative regulation ofglucose-repressible genes 0.201270.49876&radic S000001325YRB2YIL063CverifiedProtein of unknown function involved in nuclear processes ofthe Ran-GTPase cycle; involved in nuclearprotein export; contains Ran Binding Domain andFxFG repeats; interacts with Srm1p, GTP-Gsp1p,Rna1p and Crm1p; is not essential 0.200180.49701&radic&radic&radic&radic S000004047YLR057WUncharacterizedPutative protein of unknown function; YLR050C is not an essential gene 0.19220.48292&radic&radic&radic&radic&radic S000003163GCN1YGL195WverifiedPositive regulator of the Gcn2p kinase activity, forms acomplex with Gcn20p; proposed to stimulateGcn2p activation by an uncharged tRNA 0.191330.48186&radic&radic S000006385SEC23YPR181CverifiedGTPase-activating protein; component of the Sec23p-Sec24pheterodimeric complex of the COPII vesiclecoat, involved in ER to Golgi transport andautophagy; stimulates the GDP-bound form ofSar1p &radic0.188350.47736&radic&radic&radic&radic S000003365PEX4YGR133WverifiedPeroxisomal ubiquitin conjugating enzyme required forperoxisomal matrix protein import andperoxisome biogenesis 0.187480.47554&radic&radic&radic&radic&radic S000002232BRE1YDL074CverifiedE3 ubiquitin ligase for Rad6p, required for the ubiquitinationof histone H2B, recruitment of Rad6p topromoter chromatin and subsequent methylationof histone H3 (on L4 and L79), contains RINGfinger domain 0.184580.47067&radic&radic S000006177CLN2YPL256CverifiedG1 cyclin involved in regulation of the cell cycle; activatesCdc28p kinase to promote the G1 to S phasetransition; late G1 specific expression dependson transcription factor complexes, MBF(Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) 0.183180.46782&radic&radic&radic&radic S000004494RAD52YML032CverifiedProtein that stimulates strand exchange by facilitating Rad51pbinding to single-stranded DNA; annealscomplementary single-stranded DNA; involved inthe repair of double-strand breaks in DNAduring vegetative growth and meiosis 0.181560.4657&radic&radic&radic S000005583SGT1YOR057WverifiedProbable cochaperone, regulates activity of Cyr1p (adenylylcyclase); involved in assembly of thekinetochore complex, associates with the SCF(Skp1p/Cdc53p/F box protein) ubiquitin ligasecomplex 0.180780.46436&radic&radic&radic&radic&radic&radic S000002284CDC48YDL126CverifiedATPase in ER, nuclear membrane and cytosol with homology tomammalian p97; in a complex with Npl4p andUfd1p participates in retrotranslocation ofubiquitinated proteins from the ER into thecytosol for degradation by the proteasome &radic0.176640.45782&radic&radic&radic&radic S000002546RUB1YDR139CverifiedUbiquitin-like protein with similarity to mammalian NEDD8;conjugation (neddylation) substrates includethe cullins Cdc53p, Rtt101p, and Cul3p;activated by Ula1p and Uba3p (E1 enzyme pair);conjugation mediated by Ubc12p (E2 enzyme) 0.1760.45651&radic&radic&radic S000004138PEP3YLR148WverifiedVacuolar peripheral membrane protein that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, required for vacuolarbiogenesis, forms complex with Pep5p thatmediates protein transport to the vacuole 0.175620.45554&radic&radic&radic&radic S000004994SFB2YNL049CverifiedProbable component of COPII coated vesicles that binds toSec23p; similar to and functionally redundantwith Sec24p, but expressed at low levels;involved in ER to Golgi transport and inautophagy 0.175450.45513 S000004327NUP2YLR335WverifiedProtein involved in nucleocytoplasmic transport, binds toeither the nucleoplasmic or cytoplasmic facesof the nuclear pore complex depending onRan-GTP levels; also has a role in chromatinorganization 0.17450.45335&radic S000006111NAB3YPL190CverifiedSingle stranded DNA binding protein; acidic ribonucleoprotein;required for termination of non-poly(A)transcripts and efficient splicing; interactswith Nrd1p 0.170750.44663&radic S000002668SWM1YDR260CverifiedSubunit of the anaphase-promoting complex, which is an E3ubiquitin ligase that regulates themetaphase-anaphase transition and exit frommitosis; required for activation of thedaughter-specific gene expression and sporewall maturation &radic0.17050.44607&radic S000002840NPL3YDR432WverifiedRNA-binding protein that carries poly(A)+ mRNA from the nucleusinto the cytoplasm; phosphorylation by Sky1p inthe cytoplasm may promote release of mRNAs 0.169370.44415&radic&radic&radic&radic&radic&radic&radic S000005866UBC11YOR339CverifiedUbiquitin-conjugating enzyme most similar in sequence toXenopus ubiquitin-conjugating enzyme E2-C, butnot a true functional homolog of this E2;unlike E2-C, not required for the degradationof mitotic cyclin Clb2 0.168740.44286&radic&radic&radic S000004802SGS1YMR190CverifiedNucleolar DNA helicase of the RecQ family involved inmaintenance of genome integrity, regulateschromosome synapsis and meiotic crossing over;has similarity to human BLM and WRN helicasesimplicated in Bloom and Werner syndromes 0.16850.44261&radic&radic&radic&radic&radic&radic S000001208PRP8YHR165CverifiedComponent of the U4/U6-U5 snRNP complex, involved in the secondcatalytic step of splicing 0.168420.44237&radic&radic&radic S000004370SEC61YLR378CverifiedEssential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p);forms a channel for SRP-dependent proteinimport and retrograde transport of misfoldedproteins out of the ER; with Sec63 complexallows SRP-independent protein import into ER 0.165440.4367&radic&radic&radic&radic&radic S000002701SSD1YDR293CverifiedProtein with a role in maintenance of cellular integrity,interacts with components of the TOR pathway;ssd1 mutant of a clinical S. cerevisiae straindisplays elevated virulence 0.162310.43135&radic&radic&radic&radic&radic&radic&radic S000003844CSN12YJR084WverifiedSubunit of the Cop9 signalosome, which is required fordeneddylation, or removal of the ubiquitin-likeprotein Rub1p from Cdc53p (cullin); involved inadaptation to pheromone signaling 0.162110.43114&radic&radic S000003280UFD1YGR048WverifiedProtein that interacts with Cdc48p and Npl4p, involved inrecognition of polyubiquitinated proteins andtheir presentation to the 26S proteasome fordegradation; involved in transporting proteinsfrom the ER to the cytosol &radic0.161750.43046&radic&radic&radic S000003571KAR2YJL034WverifiedATPase involved in protein import into the ER, also acts as achaperone to mediate protein folding in the ERand may play a role in ER export of solubleproteins; regulates the unfolded proteinresponse via interaction with Ire1p &radic0.159070.42527 S000002728SWA2YDR320CverifiedAuxilin-like protein involved in vesicular transport;clathrin-binding protein required for uncoatingof clathrin-coated vesicles 0.15670.42078&radic&radic&radic&radic&radic&radic S000000663SED4YCR067CverifiedIntegral endoplasmic reticulum membrane protein, functions as apositive regulator of Sar1p probably throughinhibition of GTPase activation by Sec23p;binds Sec16p, participates in vesicleformation, similar to Sec12p 0.155220.41764 S000003517ZUO1YGR285CverifiedCytosolic ribosome-associated chaperone that acts, togetherwith Ssz1p and the Ssb proteins, as a chaperonefor nascent polypeptide chains; contains a DnaJdomain and functions as a J-protein partner forSsb1p and Ssb2p 0.154440.41609&radic&radic&radic&radic&radic&radic S000003065SRM1YGL097WverifiedNucleotide exchange factor for Gsp1p, localizes to the nucleus,required for nucleocytoplasmic trafficking ofmacromolecules; potentially phosphorylated byCdc28p 0.15360.41441&radic S000000738UBC8YEL012WverifiedUbiquitin-conjugating enzyme that negatively regulatesgluconeogenesis by mediating theglucose-induced ubiquitination offructose-1,6-bisphosphatase (FBPase);cytoplasmic enzyme that catalyzes theubiquitination of histones in vitro &radic0.152770.4129&radic&radic&radic S000002495SLU7YDR088CverifiedRNA splicing factor, required for ATP-independent portion of2nd catalytic step of spliceosomal RNAsplicing; interacts with Prp18p; contains zincknuckle domain 0.152570.41253&radic&radic&radic&radic S000001359FYV10YIL097WverifiedProtein of unknown function, required for survival uponexposure to K1 killer toxin; involved inproteasome-dependent catabolite inactivation offructose-1,6-bisphosphatase; contains CTLHdomain 0.150560.40939&radic&radic&radic&radic S000001587GFA1YKL104CverifiedGlutamine-fructose-6-phosphate amidotransferase, catalyzes theformation of glucosamine-6-P and glutamate fromfructose-6-P and glutamine in the first step ofchitin biosynthesis 0.149870.408&radic S000005801RIM20YOR275CverifiedProtein involved in proteolytic activation of Rim101p inresponse to alkaline pH; member of thePalA/AIP1/Alix family; interacts with theESCRT-III subunits Snf7p, suggesting arelationship between the response to pH andmultivesicular body formation 0.146510.40166&radic&radic S000004061RGR1YLR071CverifiedComponent of RNA polymerase II holoenzyme/mediator complex;affects chromatin structure and transcriptionalregulation of diverse genes; required forglucose repression, HO repression, RME1repression and sporulation 0.146010.4007&radic&radic&radic&radic&radic S000002296ARF2YDL137WverifiedADP-ribosylation factor, GTPase of the Ras superfamily involvedin regulation of coated formation vesicles inintracellular trafficking within the Golgi;functionally interchangeable with Arf1p 0.143730.39647&radic&radic&radic&radic&radic S000003892MNS1YJR131WverifiedAlpha-1,2-mannosidase involved in ER quality control; catalyzesthe removal of one mannose residue fromMan9GlcNAc to produce a single isomer ofMan8GlcNAc in N-linked oligosaccharidebiosynthesis; integral to ER membrane &radic0.14330.39568 S000001464MND2YIR025WverifiedSubunit of the anaphase-promoting complex (APC); needed formeiotic nuclear division &radic0.142080.39279&radic&radic&radic S000004118DCN1YLR128WverifiedPutative Nedd8 ligase; binds Nedd8; involved in cullinneddylation; not essential; similar toC.elegans DCN-1; contains UBA-likeubiquitin-binding domain and a DUF298 domain 0.14070.39031&radic&radic&radic S000001602VPH2YKL119CverifiedIntegral membrane protein required for vacuolar H+-ATPase(V-ATPase) function, although not an actualcomponent of the V-ATPase complex; functions inthe assembly of the V-ATPase; localized to theendoplasmic reticulum (ER) 0.140320.3894&radic&radic&radic&radic S000001068PPA1YHR026WverifiedSubunit c'' of the vacuolar ATPase, which functions inacidification of the vacuole; one of threeproteolipid subunits of the V0 domain 0.139340.38759&radic S000002819DFM1YDR411CuncharacterizedER localized derlin-like family member involved in ER stressand homeostasis; not involved in ERAD orsubstrate retrotranslocation; interacts withCDC48; contains four transmembrane domains andtwo SHP boxes &radic0.137630.38489&radic&radic&radic S000006435DID2YKR035W-AverifiedClass E protein of the vacuolar protein-sorting (Vps) pathway,associates reversibly with the late endosome,has human ortholog that may be altered inbreast tumors 0.137410.3845 S000003586MTR4YJL050WverifiedDead-box family ATP dependent helicase required for mRNA exportfrom the nucleus; co-factor of the exosomecomplex, required for 3' end formation of 5.8SrRNA &radic0.137410.3845&radic S000001542MPE1YKL059CverifiedEssential conserved subunit of CPF (cleavage andpolyadenylation factor), plays a role in 3' endformation of mRNA via the specific cleavage andpolyadenylation of pre-mRNA, contains aputative RNA-binding zinc knuckle motif 0.137240.38411&radic&radic&radic S000000918SLX8YER116CverifiedProtein containing a RING finger domain that interacts withHex3p; mutant phenotypes and geneticinteractions suggest a role in sumoylation andin genome stability 0.135490.38085&radic&radic&radic S000001932CDC26YFR036WverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.135140.38004&radic&radic&radic&radic&radic&radic S000000405DER1YBR201WverifiedEndoplasmic reticulum membrane protein, required forER-associated protein degradation of misfoldedor unassembled proteins; N- and C- terminiprotrude into the cytoplasm, has similarity toDfm1p &radic0.134330.37851&radic&radic&radic&radic S000001925PTR3YFR029WverifiedComponent of the SPS plasma membrane amino acid sensor system(Ssy1p-Ptr3p-Ssy5p), which senses externalamino acid concentration and transmitsintracellular signals that result in regulationof expression of amino acid permease genes 0.133930.37759 S000007235SEM1YDR363W-AverifiedComponent of the lid subcomplex of the regulatory subunit ofthe 26S proteasome; ortholog of human DSS1 &radic0.133110.37623&radic S000005215BNI1YNL271CverifiedFormin, nucleates the formation of linear actin filaments,involved in cell processes such as budding andmitotic spindle orientation which require theformation of polarized actin cables,functionally redundant with BNR1 0.132720.37528&radic&radic&radic S000006005BRO1YPL084WverifiedCytoplasmic class E vacuolar protein sorting (VPS) factor thatcoordinates deubiquitination in themultivesicular body (MVB) pathway by recruitingDoa4p to endosomes &radic0.132210.37416&radic&radic&radic&radic&radic S000003174CHC1YGL206CverifiedClathrin heavy chain, subunit of the major coat proteininvolved in intracellular protein transport andendocytosis; two heavy chains form the clathrintriskelion structural component; the lightchain (CLC1) is thought to regulate function 0.131850.37356&radic S000000548PDI1YCL043CverifiedProtein disulfide isomerase, multifunctional protein residentin the endoplasmic reticulum lumen, essentialfor the formation of disulfide bonds insecretory and cell-surface proteins,unscrambles non-native disulfide bonds 0.13150.37297&radic&radic S000005966VPS16YPL045WverifiedSubunit of the homotypic vacuole fusion and vacuole proteinsorting (HOPS) complex; part of the Class C Vpscomplex essential for membrane docking andfusion at both the Golgi-to-endosome andendosome-to-vacuole stages of protein transport 0.130620.37115&radic&radic&radic&radic&radic S000004092APC9YLR102CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.129890.36959&radic&radic&radic&radic&radic&radic&radic S000005008YDJ1YNL064CverifiedProtein chaperone involved in regulation of the HSP90 and HSP70functions; involved in protein translocationacross membranes; member of the DnaJ family &radic0.129560.36889&radic&radic&radic S000004106MSL5YLR116WverifiedComponent of the commitment complex, which defines the firststep in the splicing pathway; essential proteinthat interacts with Mud2p and Prp40p, forming abridge between the intron ends; also involvedin nuclear retention of pre-mRNA 0.129460.36855&radic&radic&radic&radic S000005021APJ1YNL077WverifiedPutative chaperone of the HSP40 (DNAJ) family; overexpressioninterferes with propagation of the [Psi+]prion; the authentic, non-tagged protein isdetected in highly purified mitochondria inhigh-throughput studies 0.128430.36678&radic&radic&radic S000002354SEC31YDL195WverifiedEssential phosphoprotein component (p150) of the COPII coat ofsecretory pathway vesicles, in complex withSec13p; required for ER-derived transportvesicle formation 0.128220.36643&radic S000003585YJL049WUncharacterizedPutative protein of unknown function; YJL049W is a non-essential gene 0.126370.36246&radic S000006214RPA135YPR010CverifiedRNA polymerase I subunit A135 0.125240.35988 S000000402TAF5YBR198CverifiedSubunit (90 kDa) of TFIID and SAGA complexes, involved in RNApolymerase II transcription initiation and inchromatin modification 0.12470.35865&radic&radic&radic&radic&radic S000004490TSA1YML028WverifiedUbiquitous housekeeping thioredoxin peroxidase, reducesreactive oxygen, nitrogen and sulfur speciesusing thioredoxin as hydrogen donor; mediatesredox regulation of the nuclear localization ofYap1p; deletion results in mutator phenotype 0.124560.35831&radic&radic&radic&radic S000001878MDJ1YFL016CverifiedProtein involved in folding of mitochondrially synthesizedproteins in the mitochondrial matrix; localizesto the mitochondrial inner membrane; member ofthe DnaJ family of molecular chaperones 0.124570.35831 S000005775APC5YOR249CverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.123920.35719&radic&radic&radic S000003096CWC23YGL128CverifiedComponent of a complex containing Cef1p, putatively involved inpre-mRNA splicing; has similarity to E. coliDnaJ and other DnaJ-like proteins and to S.pombe Cwf23p 0.123730.35682&radic&radic S000003863VPS25YJR102CverifiedComponent of the ESCRT-II complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome &radic0.123480.35651&radic&radic S000001157DMA1YHR115CverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma2p;homolog of S. pombe Dma1 and H. sapiens Chfr 0.12320.3562&radic&radic&radic&radic&radic S000002344TFP1YDL185WverifiedVacuolar ATPase V1 domain subunit A containing the catalyticnucleotide binding sites; protein precursorundergoes self-catalyzed splicing to yield theextein Tfp1p and the intein Vde (PI-SceI),which is a site-specific endonuclease 0.12310.35597&radic&radic S000001517TUL1YKL034WverifiedGolgi-localized RING-finger ubiquitin ligase (E3), involved inubiquitinating and sorting membrane proteinsthat contain polar transmembrane domains tomultivesicular bodies for delivery to thevacuole for quality control purposes &radic0.121870.35301&radic&radic&radic&radic S000000757SPF1YEL031WverifiedP-type ATPase, ion transporter of the ER membrane involved inER function and Ca2+ homeostasis; required forregulating Hmg2p degradation; conferssensitivity to a killer toxin (SMKT) producedby Pichia farinosa KK1 0.120740.35061&radic&radic&radic&radic&radic&radic S000002767VID21YDR359CverifiedComponent of the NuA4 histone acetyltransferase complex 0.120610.35024&radic&radic&radic S000004315CWC24YLR323CverifiedEssential protein, component of a complex containing Cef1p; hassimilarity to S. pombe Cwf24p 0.120510.35004&radic&radic S000005067NMA111YNL123WverifiedProtein of unknown function which may contribute to lipidhomeostasis and/or apoptosis; sequencesimilarity to the mammalian Omi/HtrA2 family ofserine proteases 0.119490.34787&radic S000001123LRP1YHR081WverifiedSubstrate-specific nuclear cofactor for exosome activity in theprocessing of stable RNAs; required fortelomere length maintenance; homolog ofmammalian nuclear matrix protein C1D involvedin regulation of DNA repair and recombination 0.119460.34781&radic&radic S000004568CUE4YML101CuncharacterizedProtein of unknown function; has a CUE domain that bindsubiquitin, which may facilitate intramolecularmonoubiquitination 0.118990.34648&radic S000004844PEP5YMR231WverifiedPeripheral vacuolar membrane protein required for proteintrafficking and vacuole biogenesis; formscomplex with Pep3p that promotes vesiculardocking/fusion reactions in conjunction withSNARE proteins, also interacts with Pep7p 0.118760.34607&radic S000003149GTS1YGL181WverifiedProtein containing a zinc-finger in the N-terminus and a longGln-rich region in the C-terminus; regulatesultradian rhythm, cell size, cell cycle,lifespan, sporulation, heat tolerance, andmultidrug transport 0.118420.34523&radic S000005735NPT1YOR209CverifiedNicotinate phosphoribosyltransferase, acts in the salvagepathway of NAD+ biosynthesis; required forsilencing at rDNA and telomeres and has a rolein silencing at mating-type loci; localized tothe nucleus 0.118360.34507&radic&radic&radic&radic&radic&radic&radic S000000777VMA8YEL051WverifiedSubunit D of the eight-subunit V1 peripheral membrane domain ofthe vacuolar H+-ATPase (V-ATPase), anelectrogenic proton pump found throughout theendomembrane system; plays a role in thecoupling of proton transport and ATP hydrolysis 0.117920.34441&radic&radic&radic&radic S000004080XDJ1YLR090WverifiedPutative chaperone, homolog of E. coli DnaJ, closely related toYdj1p; the authentic, non-tagged protein isdetected in highly purified mitochondria inhigh-throughput studies 0.117140.34279&radic&radic&radic&radic&radic&radic S000002824SYF1YDR416WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression; similar to Drosophila crooked neckprotein 0.11690.34233&radic&radic&radic&radic&radic S000002894VPS60YDR486CverifiedCytoplasmic and vacuolar membrane protein involved in lateendosome to vacuole transport; required fornormal filament maturation during pseudohyphalgrowth; may function in targeting specificcargo proteins for degradation 0.115680.33977&radic S000004861RKR1YMR247CverifiedProtein of unknown function; may interact with ribosomes, basedon co-purification experiments; YMR247C is notan essential gene 0.115430.33946&radic&radic&radic&radic S000002299RPO21YDL140CverifiedRNA polymerase II largest subunit B220, part of central core;phosphorylation of C-terminal heptapeptiderepeat domain regulates association withtranscription and splicing factors; similar tobacterial beta-prime 0.115060.3383&radic&radic S000005378TLG2YOL018CverifiedSyntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1pand mediates fusion of endosome-derivedvesicles with the late Golgi; binds Vps45p,which prevents Tlg2p degradation and alsofacilitates t-SNARE complex formation 0.113880.33605&radic&radic&radic&radic&radic S000004069SIC1YLR079WverifiedInhibitor of Cdc28-Clb kinase complexes that controls G1/Sphase transition, preventing premature S phaseand ensuring genomic integrity; phosphorylationtargets Sic1p for SCF(CDC4)-dependent turnover;functional homolog of mammalian Kip1 0.113790.33585&radic&radic&radic&radic&radic S000003461SMI1YGR229CverifiedProtein involved in the regulation of cell wall synthesis;proposed to be involved in coordinating cellcycle progression with cell wall integrity 0.113780.33585&radic&radic&radic&radic&radic S000004107CLF1YLR117CverifiedEssential splicesome assembly factor; contains multipletetratricopeptide repeat (TPR) protein-bindingmotifs and interacts specifically with manyspliceosome components, may serve as a scaffoldduring splicesome assembly 0.112970.3336&radic&radic&radic&radic S000000377UMP1YBR173CverifiedShort-lived chaperone required for correct maturation of the20S proteasome; degraded by proteasome uponcompletion of its assembly; involved inubiquitin-mediated proteolysis; mutantdefective in degradation of short-livedproteins &radic0.113030.3336&radic&radic&radic&radic&radic&radic&radic&radic S000006072PRP46YPL151CverifiedSplicing factor that is found in the Cef1p subcomplex of thespliceosome 0.11180.3309&radic&radic&radic&radic&radic S000004924GAS1YMR307WverifiedBeta-1.3-glucanosyltransferase, required for cell wallassembly; localizes to the cell surface via aglycosylphosphatidylinositol (GPI) anchor 0.11180.3309&radic&radic&radic S000006161HSP82YPL240CverifiedCytoplasmic chaperone (Hsp90 family) required for pheromonesignaling and negative regulation of Hsf1p;docks with the mitochondrial import receptorTom70p for preprotein delivery; interacts withco-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p 0.111240.32982&radic&radic&radic&radic&radic&radic S000003746PEX2YJL210WverifiedRING-finger peroxin, peroxisomal membrane protein with aC-terminal zinc-binding RING domain, formsputative translocation subcomplex with Pex10pand Pex12p which functions in peroxisomalmatrix protein import 0.111170.32964&radic&radic S000004005BRE2YLR015WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres;involved in telomere maintenance; similar totrithorax-group protein ASH2L 0.111120.32958&radic&radic&radic&radic&radic S000003609JEM1YJL073WverifiedDnaJ-like chaperone required for nuclear membrane fusion duringmating, localizes to the ER membrane; exhibitsgenetic interactions with KAR2 &radic0.109810.32635&radic S000005257YNL313CUncharacterizedProtein required for cell viability 0.109250.32507&radic&radic&radic S000004206CPR6YLR216CverifiedPeptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzesthe cis-trans isomerization of peptide bondsN-terminal to proline residues; binds to Hsp82pand contributes to chaperone activity 0.109190.32496&radic&radic&radic&radic&radic&radic S000006125HRR25YPL204WverifiedProtein kinase involved in regulating diverse events includingvesicular trafficking, gene expression, DNArepair, and chromosome segregation; binds theCTD of RNA pol II; homolog of mammalian caseinkinase 1delta (CK1delta) 0.108640.32374&radic&radic&radic&radic&radic&radic S000002456YDR049WUncharacterizedZinc finger protein; putative transcription factor that may interact with proteins involved in histone acetylation or deacetylation; may be involved in altering acetylation on histone lysines 0.108020.32224&radic&radic&radic&radic S000002464YOS9YDR057WverifiedLectin; soluble lumenal ER protein; member of the OS-9 proteinfamily; similar to mannose-6-phosphatereceptors (MPRs); serves as a receptor thatrecognizes misfolded N-glycosylated proteinsand participates in their targeting to ERAD &radic0.108030.32224&radic&radic S000005242CLA4YNL298WverifiedCdc42p activated signal transducing kinase of the PAK(p21-activated kinase) family, involved inseptin ring assembly and cytokinesis; directlyphosphorylates septins Cdc3p and Cdc10p; otheryeast PAK family members are Ste20p and Skm1p 0.107550.32103&radic&radic&radic&radic S000001338SEC28YIL076WverifiedEpsilon-COP subunit of the coatomer; regulates retrogradeGolgi-to-ER protein traffic; stabilizes Cop1p,the alpha-COP and the coatomer complex;non-essential for cell growth 0.107230.32017&radic&radic&radic S000001485DID4YKL002WverifiedClass E Vps protein of the ESCRT-III complex, required forsorting of integral membrane proteins intolumenal vesicles of multivesicular bodies, andfor delivery of newly synthesized vacuolarenzymes to the vacuole, involved in endocytosis &radic0.107150.32015&radic&radic&radic&radic S000004900DSS1YMR287CverifiedRNase, component of the mitochondrial degradosome along withthe ATP-dependent RNA helicase Suv3p; thedegradosome associates with the ribosome andmediates turnover of aberrant or unprocessedRNAs 0.107030.31977&radic&radic&radic&radic S000002371SHR3YDL212WverifiedEndoplasmic reticulum packaging chaperone, required forincorporation of amino acid permeases intoCOPII coated vesicles for transport to the cellsurface 0.106540.31905 S000005206POL2YNL262WverifiedCatalytic subunit of DNA polymerase epsilon, one of the majorchromosomal DNA replication polymerasescharacterized by processivity and proofreadingexonuclease activity; also involved in DNAsynthesis during DNA repair 0.106510.31884&radic&radic&radic&radic&radic&radic&radic S000001094CIC1YHR052WverifiedEssential protein that interacts with proteasome components andhas a potential role in proteasome substratespecificity; also copurifies with 66Spre-ribosomal particles 0.106510.31884&radic S000000994HSE1YHL002WverifiedSubunit of the endosomal Vps27p-Hse1p complex required forsorting of ubiquitinated membrane proteins intointralumenal vesicles prior to vacuolardegradation, as well as for recycling of Golgiproteins and formation of lumenal membranes 0.106140.31822 S000004390SKI2YLR398CverifiedPutative RNA helicase, involved in exosome mediated 3' to 5'mRNA degradation and translation inhibition ofnon-poly(A) mRNAs; forms complex with Ski3p andSki8p; required for repressing propagation ofdsRNA viruses 0.105970.3178&radic&radic&radic&radic S000002326NRP1YDL167CverifiedProtein of unknown function, rich in asparagine residues 0.105130.31569&radic S000005868RPA190YOR341WverifiedRNA polymerase I subunit; largest subunit of RNA polymerase I 0.104950.31522&radic&radic&radic&radic&radic S000004237IRC20YLR247CuncharacterizedPutative helicase; localized to mitochondria and the nucleus;YLR247C is not an essential gene; null mutantdisplays increased levels of spontaneous Rad52foci 0.104550.31417&radic&radic&radic&radic&radic S000002520PDS1YDR113CverifiedSecurin that inhibits anaphase by binding separin Esp1p, alsoblocks cyclin destruction and mitotic exit,essential for cell cycle arrest in mitosis inthe presence of DNA damage or aberrant mitoticspindles; also present in meiotic nuclei 0.104420.3136&radic&radic S000004022RAD5YLR032WverifiedSingle-stranded DNA-dependent ATPase, involved inpostreplication repair; contains RING fingerdomain 0.103260.31073&radic&radic S000005664RUP1YOR138CverifiedProtein involved in regulation of Rsp5p, which is an essentialHECT ubiquitin ligase; required for binding ofRsp5p to Ubp2p; contains an UBA domain 0.103230.31055&radic&radic&radic&radic&radic&radic&radic S000004410CDC73YLR418CverifiedConstituent of Paf1 complex with RNA polymerase II, Paf1p,Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinctfrom Srb-containing Pol II complexes; requiredfor expression of certain genes, modificationof some histones, and telomere maintenance 0.103060.31021&radic&radic&radic&radic&radic S000004284GSP1YLR293CverifiedGTP binding protein (mammalian Ranp homolog) involved in themaintenance of nuclear organization, RNAprocessing and transport; regulated by Prp20p,Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p andKap95p; yeast Gsp2p homolog 0.102960.3099&radic&radic&radic S000005133SRP1YNL189WverifiedKaryopherin alpha homolog, forms a dimer with karyopherin betaKap95p to mediate import of nuclear proteins,binds the nuclear localization signal of thesubstrate during import; may also play a rolein regulation of protein degradation 0.10240.30868&radic&radic&radic&radic&radic&radic S000000262UBP14YBR058CverifiedUbiquitin-specific protease that specifically disassemblesunanchored ubiquitin chains; involved infructose-1,6-bisphosphatase (Fbp1p)degradation; similar to human isopeptidase T 0.101420.30627&radic&radic S000000064SWD1YAR003WverifiedSubunit of the COMPASS (Set1C) complex, which methylateshistone H3 on lysine 4 and is required intranscriptional silencing near telomeres; WD40beta propeller superfamily member withsimilarity to mammalian Rbbp7 0.101310.30598&radic S000000973RAD3YER171Wverified5' to 3' DNA helicase, involved in nucleotide excision repairand transcription; subunit of RNA polymerase IItranscription initiation factor TFIIH; subunitof Nucleotide Excision Repair Factor 3 (NEF3);homolog of human XPD protein 0.101290.30598&radic&radic S000006233APL4YPR029CverifiedGamma-adaptin, large subunit of the clathrin-associated protein(AP-1) complex; binds clathrin; involved invesicle mediated transport 0.101340.30598&radic S000002681DON1YDR273WverifiedMeiosis-specific component of the spindle pole body, part ofthe leading edge protein (LEP) coat, forms aring-like structure at the leading edge of theprospore membrane during meiosis II 0.101230.30581&radic&radic&radic S000001333PCI8YIL071CverifiedPossible shared subunit of Cop9 signalosome (CSN) and eIF3,binds eIF3b subunit Prt1p, has possible dualfunctions in transcriptional and translationalcontrol, contains a PCI (Proteasome-COP9signalosome (CSN)-eIF3) domain 0.10120.30579&radic&radic S000005967ELC1YPL046CverifiedElongin C, forms heterodimer with Ela1p that participates intranscription elongation; expressiondramatically upregulated during sporulation;widely conserved among eukaryotes &radic0.101040.30547&radic&radic&radic&radic&radic&radic&radic S000001292SSM4YIL030CverifiedUbiquitin-protein ligase of the ER/nuclear envelope, requiredfor degradation of Alpha2p and other proteinscontaining a Deg1 degradation signal; ssm4mutation suppresses mRNA instability caused byan rna14 mutation &radic0.100970.30531 S000002568YDR161WUncharacterizedinteracts with PP2C 0.100710.30457&radic&radic&radic&radic&radic&radic&radic S000006386SMX3YPR182WverifiedCore Sm protein Sm F; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm F 0.09930.30075&radic&radic&radic&radic S000005552BUB3YOR026WverifiedKinetochore checkpoint WD40 repeat protein that localizes tokinetochores during prophase and metaphase,delays anaphase in the presence of unattachedkinetochores; forms complexes with Mad1p-Bub1pand with Cdc20p, binds Mad2p and Mad3p 0.099110.30029&radic&radic&radic&radic&radic&radic S000004014UBR2YLR024CverifiedCytoplasmic ubiquitin-protein ligase (E3) 0.099010.30003&radic&radic&radic&radic S000000316CYC8YBR112CverifiedGeneral transcriptional co-repressor, acts together with Tup1p;also acts as part of a transcriptionalco-activator complex that recruits the SWI/SNFand SAGA complexes to promoters 0.098860.29951&radic S000000379SWD3YBR175WverifiedEssential subunit of the COMPASS (Set1C) complex, whichmethylates histone H3 on lysine 4 and isrequired in transcriptional silencing neartelomeres; WD40 beta propeller superfamilymember and ortholog of mammalian WDR5 0.09880.29941&radic&radic&radic&radic S000002625RAD9YDR217CverifiedDNA damage-dependent checkpoint protein, required forcell-cycle arrest in G1/S, intra-S, and G2/M;transmits checkpoint signal by activatingRad53p and Chk1p; hyperphosphorylated by Mec1pand Tel1p; potential Cdc28p substrate 0.098730.299&radic&radic&radic&radic&radic&radic S000001596RAD27YKL113Cverified5' to 3' exonuclease, 5' flap endonuclease, required forOkazaki fragment processing and maturation aswell as for long-patch base-excision repair;member of the S. pombe RAD2/FEN1 family 0.098590.29891&radic&radic&radic&radic&radic&radic S000002663RMD5YDR255CverifiedCytosolic protein required for sporulation; also required forthe ubiquitination of the gluconeogeneticenzyme fructose-1,6-bisphosphatase, which isdegraded rapidly after the switch fromgluconeogenesis to glycolysis 0.09840.29847&radic&radic&radic&radic S000002800SPT3YDR392WverifiedSubunit of the SAGA and SAGA-like transcriptional regulatorycomplexes, interacts with Spt15p to activatetranscription of some RNA polymeraseII-dependent genes, also functions to inhibittranscription at some promoters 0.097620.29651&radic&radic&radic&radic&radic&radic S000006006SEC16YPL085WverifiedCOPII vesicle coat protein required for ER transport vesiclebudding and autophagosome formation; Sec16p isbound to the periphery of ER membranes and mayact to stabilize initial COPII complexes;interacts with Sec23p, Sec24p and Sec31p 0.096830.29482&radic&radic&radic&radic&radic S000004926NIP1YMR309CverifiedSubunit of the eukaryotic translation initiation factor 3(eIF3), involved in the assembly ofpreinitiation complex and start codon selection 0.09660.29443&radic&radic&radic S000006124TPK2YPL203WverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; activates filamentous growth 0.096620.29443&radic&radic&radic&radic&radic S000001493UFD4YKL010CverifiedUbiquitin-protein ligase (E3) that interacts with Rpt4p andRpt6p, two subunits of the 19S particle of the26S proteasome; cytoplasmic E3 involved in thedegradation of ubiquitin fusion proteins 0.095560.29134&radic&radic&radic S000004814RAD14YMR201CverifiedProtein that recognizes and binds damaged DNA during nucleotideexcision repair; subunit of Nucleotide ExcisionRepair Factor 1 (NEF1); contains zinc fingermotif; homolog of human XPA protein 0.09550.29123&radic&radic&radic&radic&radic&radic S000003399CLC1YGR167WverifiedClathrin light chain, subunit of the major coat proteininvolved in intracellular protein transport andendocytosis; thought to regulate clathrinfunction, two Clathrin heavy chains (CHC1) formthe clathrin triskelion structural component 0.095420.29082&radic&radic&radic&radic&radic&radic S000002918SMT3YDR510WverifiedUbiquitin-like protein of the SUMO family, conjugated to lysineresidues of target proteins; regulateschromatid cohesion, chromosome segregation,APC-mediated proteolysis, DNA replication andseptin ring dynamics 0.095380.29069&radic&radic S000001728VPS51YKR020WverifiedComponent of the GARP (Golgi-associated retrograde protein)complex, Vps51p-Vps52p-Vps53p-Vps54p, which isrequired for the recycling of proteins fromendosomes to the late Golgi; links the(VFT/GARP) complex to the SNARE Tlg1p 0.094910.28946&radic&radic&radic&radic&radic S000004722ASC1YMR116CverifiedWD repeat protein (G-beta like protein) involved in translationregulation; required for repression of Gcn4pactivity in the absence of amino-acidstarvation; core component of the ribosome;ortholog of mammalian RACK1 0.094580.28824&radic&radic S000001930PHO4YFR034CverifiedBasic helix-loop-helix (bHLH) transcription factor; bindscooperatively with Pho2p to the PHO5 promoter;function is regulated by phosphorylation atmultiple sites and by phosphate availability 0.094390.28795&radic&radic&radic&radic&radic&radic S000004198SEC13YLR208WverifiedComponent of both the Nup84 nuclear pore sub-complex and of theCOPII complex (Sar1p, Sec13p, Sec16p, Sec23p,Sec24p, Sec31p, Sfb2p, and Sfb3p) which isimportant for the formation of ER to Golgitransport vesicles &radic0.094370.28788&radic&radic&radic S000004798HSC82YMR186WverifiedCytoplasmic chaperone of the Hsp90 family, redundant infunction and nearly identical with Hsp82p, andtogether they are essential; expressedconstitutively at 10-fold higher basal levelsthat HSP82 and induced 2-3 fold by heat shock 0.094340.28784&radic&radic&radic&radic&radic&radic S000002545HPR1YDR138WverifiedSubunit of THO/TREX complexes that couple transcriptionelongation with mitotic recombination and withmRNA metabolism and export, subunit of an RNAPol II complex; regulates lifespan; involved intelomere maintenance; similar to Top1p 0.094190.28724&radic&radic&radic&radic&radic S000002712CPR5YDR304CverifiedPeptidyl-prolyl cis-trans isomerase (cyclophilin) of theendoplasmic reticulum, catalyzes the cis-transisomerization of peptide bonds N-terminal toproline residues; transcriptionally induced inresponse to unfolded proteins in the ER 0.093520.2855&radic&radic&radic&radic S000005199GIS2YNL255CverifiedProtein with seven cysteine-rich CCHC zinc-finger motifs,similar to human CNBP, proposed to be involvedin the RAS/cAMP signaling pathway 0.09340.28528&radic&radic&radic&radic&radic S000001863HAC1YFL031WverifiedbZIP transcription factor (ATF/CREB1 homolog) that regulatesthe unfolded protein response, via UPREbinding, and membrane biogenesis; ERstress-induced splicing pathway utilizingIre1p, Trl1p and Ada5p facilitates efficientHac1p synthesis 0.093140.28464&radic&radic&radic&radic S000001937ERJ5YFR041CuncharacterizedType I membrane protein with a J domain is required to preservethe folding capacity of the endoplasmicreticulum; loss of the non-essential ERJ5 geneleads to a constitutively induced unfoldedprotein response 0.092560.28307&radic&radic&radic&radic S000003963VPS13YLL040CverifiedProtein of unknown function; heterooligomeric or homooligomericcomplex; peripherally associated withmembranes; homologous to human COH1; involvedin sporulation, vacuolar protein sorting andprotein-Golgi retention 0.092430.28289&radic&radic&radic&radic&radic S000001121IRE1YHR079CverifiedSerine-threonine kinase and endoribonuclease; transmembraneprotein that initiates the unfolded proteinresponse signal by regulating synthesis ofHac1p through HAC1 mRNA splicing 0.092340.28259&radic&radic&radic S000000602RVS161YCR009CverifiedAmphiphysin-like lipid raft protein; subunit of a complex(Rvs161p-Rvs167p) that regulates polarizationof the actin cytoskeleton, endocytosis, cellpolarity, cell fusion and viability followingstarvation or osmotic stress 0.091560.28042&radic&radic S000004329VRP1YLR337CverifiedProline-rich actin-associated protein involved in cytoskeletalorganization and cytokinesis; related tomammalian Wiskott-Aldrich syndrome protein(WASP)-interacting protein (WIP) 0.091260.27965&radic&radic&radic&radic S000002566SAC3YDR159WverifiedNuclear pore-associated protein, forms a complex with Thp1pthat is involved in transcription and in mRNAexport from the nucleus 0.090230.27663&radic&radic&radic S000002777XRS2YDR369CverifiedProtein required for DNA repair; component of the Mre11complex, which is involved in double strandbreaks, meiotic recombination, telomeremaintenance, and checkpoint signaling 0.090190.27646&radic&radic&radic&radic&radic S000003215RAI1YGL246CverifiedNuclear protein that binds to and stabilizes theexoribonuclease Rat1p, required for pre-rRNAprocessing 0.089740.27531&radic&radic&radic&radic&radic&radic S000002721PIB1YDR313CverifiedRING-type ubiquitin ligase of the endosomal and vacuolarmembranes, bindsphosphatidylinositol(3)-phosphate; contains aFYVE finger domain 0.089430.27437&radic&radic&radic&radic&radic S000004197HRD3YLR207WverifiedResident protein of the ER membrane that plays a central rolein ER-associated protein degradation (ERAD),forms HRD complex with Hrd1p and ERADdeterminants that engages in lumen to cytosolcommunication and coordination of ERAD events &radic0.089380.27428&radic S000000885GET2YER083CverifiedSubunit of the GET complex; required for meiotic nucleardivision and for the retrieval of HDEL proteinsfrom the Golgi to the ER in an ERD2 dependentfashion; may be involved in cell wall function 0.089090.27368&radic&radic&radic&radic S000004214YLR224WUncharacterizedF-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene &radic0.088820.27303&radic&radic&radic&radic S000003794RAV1YJR033CverifiedSubunit of the RAVE complex (Rav1p, Rav2p, Skp1p), whichpromotes assembly of the V-ATPase holoenzyme;required for transport between the early andlate endosome/PVC and for localization of TGNmembrane proteins; potential Cdc28p substrate 0.088810.27277 S000004700CTF13YMR094WverifiedSubunit of the CBF3 complex, which binds to the CDE III elementof centromeres, bending the DNA upon binding,and may be involved in sister chromatidcohesion during mitosis 0.088480.27181 S000004109SRN2YLR119WverifiedComponent of the ESCRT-I complex, which is involved inubiquitin-dependent sorting of proteins intothe endosome; suppressor of rna1-1 mutation;may be involved in RNA export from nucleus &radic0.088230.27129&radic&radic&radic S000003698JJJ2YJL162CuncharacterizedProtein of unknown function, contains a J-domain, which is aregion with homology to the E. coli DnaJprotein 0.087920.27045&radic&radic&radic S000006368MMS1YPR164WverifiedProtein likely involved in protection againstreplication-dependent DNA damage; mutants aresensitive to methyl methanesulfonate (MMS);implicated in regulation of Ty1 transposition 0.087080.26845&radic&radic S000000682CSM1YCR086WverifiedNucleolar protein that forms a complex with Lrs4p which bindsMam1p at kinetochores during meiosis I tomediate accurate chromosome segregation, may beinvolved in premeiotic DNA replication;possible role in telomere maintenance 0.086920.26784&radic&radic S000002178RPN4YDL020CverifiedTranscription factor that stimulates expression of proteasomegenes; Rpn4p levels are in turn regulated bythe 26S proteasome in a negative feedbackcontrol mechanism; RPN4 is transcriptionallyregulated by various stress responses 0.086890.26784&radic&radic&radic S000004045SPT8YLR055CverifiedSubunit of the SAGA transcriptional regulatory complex but notpresent in SAGA-like complex SLIK/SALSA,required for SAGA-mediated inhibition at somepromoters 0.086630.26677&radic&radic&radic&radic&radic&radic S000001725YKR017CUncharacterizedPutative protein of unknown function; contains a RING finger motif 0.086190.26599&radic&radic&radic S000002652PEX5YDR244WverifiedPeroxisomal membrane signal receptor for C-terminal tripeptidesignal sequence (PTS1) of peroxisomal matrixproteins, required for peroxisomal matrixprotein import, tetratricopeptide repeatprotein, also involved in PTS1-independentimport 0.086150.26589&radic&radic&radic S000003813RAD7YJR052WverifiedProtein that recognizes and binds damaged DNA in anATP-dependent manner (with Rad16p) duringnucleotide excision repair; subunit ofNucleotide Excision Repair Factor 4 (NEF4) 0.085350.26347&radic&radic S000003372CBF2YGR140WverifiedEssential kinetochore protein, component of the CBF3multisubunit complex that binds to the CDEIIIregion of the centromere; Cbf2p also binds tothe CDEII region possibly forming a differentmultimeric complex, ubiquitinated in vivo 0.085350.26347&radic&radic&radic&radic&radic S000004467ERG6YML008CverifiedDelta(24)-sterol C-methyltransferase, converts zymosterol tofecosterol in the ergosterol biosyntheticpathway by methylating position C-24; localizedto both lipid particles and mitochondrial outermembrane 0.085240.26347&radic&radic&radic&radic&radic&radic S000006311YTH1YPR107CverifiedEssential RNA-binding component of cleavage and polyadenylationfactor, contains five zinc fingers; requiredfor pre-mRNA 3'-end processing andpolyadenylation 0.085130.26303&radic&radic&radic&radic&radic S000005814MPD1YOR288CverifiedMember of the protein disulfide isomerase (PDI) family;interacts with and inhibits the chaperoneactivity of Cne1p; MPD1 overexpression in apdi1 null mutant suppresses defects in Pdi1pfunctions such as carboxypeptidase Y maturation 0.084940.26247&radic S000005366TOP1YOL006CverifiedTopoisomerase I, nuclear enzyme that relieves torsional strainin DNA by cleaving and re-sealing thephosphodiester backbone; relaxes bothpositively and negatively supercoiled DNA;functions in replication, transcription, andrecombination 0.084750.26216&radic&radic&radic&radic S000000753CUP5YEL027WverifiedProteolipid subunit of the vacuolar H(+)-ATPase V0 sector(subunit c; dicyclohexylcarbodiimide bindingsubunit); required for vacuolar acidificationand important for copper and iron metal ionhomeostasis 0.084470.26127&radic S000002362ACK1YDL203CuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.084030.26025 S000004603CDC5YMR001CverifiedPolo-like kinase with similarity to Xenopus Plx1 and S. pombePlo1p; found at bud neck, nucleus and SPBs; hasmultiple functions in mitosis and cytokinesisthrough phosphorylation of substrates; may be aCdc28p substrate 0.083910.25981&radic&radic&radic&radic S000000143EDE1YBL047CverifiedKey endocytic protein involved in a network of interactionswith other endocytic proteins, binds membranesin a ubiquitin-dependent manner, may also bindubiquitinated membrane-associated proteins 0.08350.25879&radic&radic&radic S000004842RRP5YMR229CverifiedProtein required for the synthesis of both 18S and 5.8S rRNA;C-terminal region is crucial for the formationof 18S rRNA and N-terminal region is requiredfor the 5.8S rRNA; component of small ribosomalsubunit (SSU) processosome 0.083480.2584&radic&radic&radic&radic S000000364CDC28YBR160WverifiedCatalytic subunit of the main cell cycle cyclin-dependentkinase (CDK); alternately associates with G1cyclins (CLNs) and G2/M cyclins (CLBs) whichdirect the CDK to specific substrates 0.083370.25826&radic&radic S000002171HEX3YDL013WverifiedProtein containing a RING finger domain that interacts withSlx8p; mutant phenotypes and geneticinteractions suggest a role in sumoylation andin genome stability 0.083110.25755&radic&radic S000005060DMA2YNL116WverifiedProtein involved in regulating spindle position andorientation, functionally redundant with Dma1p;homolog of S. pombe Dma1 and H. sapiens Chfr 0.083030.25738&radic&radic S000004527MFT1YML062CverifiedSubunit of the THO complex, which is a nuclear complexcomprised of Hpr1p, Mft1p, Rlr1p, and Thp2p,that is involved in transcription elongationand mitotic recombination; involved in telomeremaintenance 0.082860.25687&radic&radic S000000850CAJ1YER048CverifiedNuclear type II J heat shock protein of the E. coli dnaJfamily, contains a leucine zipper-like motif,binds to non-native substrates for presentationto Ssa3p, may function during proteintranslocation, assembly and disassembly 0.082880.25687&radic&radic&radic S000000975RAD24YER173WverifiedCheckpoint protein, involved in the activation of the DNAdamage and meiotic pachytene checkpoints;subunit of a clamp loader that loadsRad17p-Mec3p-Ddc1p onto DNA; homolog of humanand S. pombe Rad17 protein 0.082650.25629&radic&radic S000005642RPO31YOR116CverifiedRNA polymerase III subunit C160, part of core enzyme; similarto bacterial beta-prime subunit 0.082570.2562&radic&radic&radic S000002789YRA1YDR381WverifiedNuclear protein that binds to RNA and to Mex67p, required forexport of poly(A)+ mRNA from the nucleus;member of the REF (RNA and export factorbinding proteins) family; another familymember, Yra2p, can substitute for Yra1pfunction 0.082360.25574&radic&radic&radic&radic&radic S000003175SPT16YGL207WverifiedSubunit of the heterodimeric FACT complex (Spt16p-Pob3p),facilitates RNA Polymerase II transcriptionelongation through nucleosomes by destabilizingand then reassembling nucleosome structure 0.082270.25533&radic&radic&radic&radic&radic S000004837MRE11YMR224CverifiedSubunit of a complex with Rad50p and Xrs2p (RMX complex) thatfunctions in repair of DNA double-strand breaksand in telomere stability, exhibits nucleaseactivity that appears to be required for RMXfunction; widely conserved 0.082250.25532&radic&radic&radic&radic&radic&radic S000004813ROT1YMR200WverifiedEssential ER membrane protein; may be involved in proteinfolding; mutation causes defects in cell wallsynthesis and in lysis of autophagic bodies,suppresses tor2 mutations, and is syntheticallylethal with kar2-1 and with rot2 mutations 0.081370.25281&radic S000004781YMR171CUncharacterizedProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to endosomes; mRNA is targeted to the bud via the mRNA transport system involving She2p: YMR171C is not an essential gene 0.081330.25262 S000002544RGP1YDR137WverifiedSubunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) thatcatalyzes nucleotide exchange on Ypt6p 0.080950.25149&radic S000002444KRS1YDR037WverifiedLysyl-tRNA synthetase; also identified as a negative regulatorof general control of amino acid biosynthesis 0.080950.25149&radic S000004901HSH155YMR288WverifiedU2-snRNP associated splicing factor that forms extensiveassociations with the branch site-3' splicesite-3' exon region upon prespliceosomeformation; similarity to the mammalian U2snRNP-associated splicing factor SAP155 0.079270.24681&radic&radic&radic&radic&radic&radic&radic S000005415PSH1YOL054WuncharacterizedNuclear protein, putative RNA polymerase II elongation factor;isolated as Pob3p/Spt16p-binding protein 0.078880.24587&radic&radic S000004411YLR419WUncharacterizedPutative helicase with limited sequence similarity to human Rb protein; localized to the cytoplasm and the mitochondria; YLR419W is not an essential gene 0.078620.24516&radic S000001607SSH4YKL124WverifiedProtein whose overexpression confers resistance to the growthinhibitor leflunomide; suppressor of shr3mutation 0.078240.24428&radic&radic&radic&radic S000003998PAM18YLR008CverifiedConstituent of the mitochondrial import motor associated withthe presequence translocase, along with Ssc1p,Tim44p, Mge1p, and Pam16p; stimulates theATPase activity of Ssc1p to drive mitochondrialimport; contains a J domain 0.078120.24399&radic&radic&radic S000002719TFB1YDR311WverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, required for nucleotide excisionrepair, target for transcriptional activators 0.077840.24323&radic&radic&radic&radic&radic&radic S000003576NUP192YJL039CverifiedEssential structural subunit of the nuclear pore complex (NPC),localizes to the nuclear periphery of nuclearpores, homologous to human p205 0.077750.243 S000004339KAP95YLR347CverifiedKaryopherin beta, forms a dimeric complex with Srp1p (Kap60p)that mediates nuclear import of cargo proteinsvia a nuclear localization signal (NLS),interacts with nucleoporins to guide transportacross the nuclear pore complex 0.077780.243&radic&radic&radic&radic&radic&radic S000005182KEX2YNL238WverifiedSubtilisin-like protease (proprotein convertase), acalcium-dependent serine protease involved inthe activation of proproteins of the secretorypathway 0.077710.2427&radic&radic&radic&radic S000002525APC4YDR118WverifiedSubunit of the Anaphase-Promoting Complex/Cyclosome (APC/C),which is a ubiquitin-protein ligase requiredfor degradation of anaphase inhibitors,including mitotic cyclins, during themetaphase/anaphase transition &radic0.077160.24143&radic&radic&radic&radic&radic S000001236EGD2YHR193CverifiedAlpha subunit of the heteromeric nascent polypeptide-associatedcomplex (NAC) involved in protein sorting andtranslocation, associated with cytoplasmicribosomes 0.076850.24042&radic&radic S000006169GAL4YPL248CverifiedDNA-binding transcription factor required for the activation ofthe GAL genes in response to galactose;repressed by Gal80p and activated by Gal3p 0.076840.2404&radic&radic&radic S000004239YEF3YLR249WverifiedTranslational elongation factor, stimulates the binding ofaminoacyl-tRNA (AA-tRNA) to ribosomes byreleasing EF-1 alpha from the ribosomalcomplex; contains two ABC cassettes; binds andhydrolyses ATP 0.076670.23992&radic&radic&radic&radic&radic&radic&radic S000002743MSN5YDR335WverifiedKaryopherin involved in nuclear import and export; shown to beresponsible for nuclear import of replicationprotein A and for export of several proteinsincluding Swi6p, Far1p, and Pho4p; cargodissociation involves binding to RanGTP 0.075840.23764&radic&radic&radic&radic&radic&radic&radic S000000103SLA1YBL007CverifiedCytoskeletal protein binding protein required for assembly ofthe cortical actin cytoskeleton; contains 3 SH3domains; interacts with proteins regulatingactin dynamics and with proteins required forendocytosis 0.075160.23582&radic&radic&radic&radic S000004563VPS9YML097CverifiedA guanine nucleotide exchange factor involved invesicle-mediated vacuolar protein transport;specifically stimulates the intrinsic guaninenucleotide exchange activity of Vps21p/Rab5:similar to mammalian ras inhibitors; bindsubiquitin 0.075110.23567&radic&radic S000001443DJP1YIR004WverifiedCytosolic J-domain-containing protein, required for peroxisomalprotein import and involved in peroxisomeassembly, homologous to E. coli DnaJ 0.075040.23548 S000005171JJJ1YNL227CverifiedProtein that contains a 70 amino acid J-domain, may function asa co-chaperone to recruit Hsp70-like activityto specific sites; mutation causes defects influid-phase endocytosis 0.075040.23548&radic&radic&radic&radic&radic&radic S000005601UFE1YOR075Wverifiedt-SNARE required for ER membrane fusion and vesicular traffic,integral membrane protein that constitutes withSec20p and Use1p the trimeric acceptor forR/v-SNAREs on Golgi-derived vesicles at the ER;part of Dsl1p complex 0.074970.23523&radic&radic&radic&radic S000000533RNQ1YCL028Wverified[PIN(+)] prion, an infectious protein conformation that isgenerally an ordered protein aggregate 0.074650.23431&radic&radic&radic&radic&radic&radic&radic S000000157SKT5YBL061CverifiedActivator of Chs3p (chitin synthase III), recruits Chs3p to thebud neck via interaction with Bni4p; hassimilarity to Shc1p, which activates Chs3pduring sporulation 0.074240.23321&radic&radic&radic&radic&radic S000004157RPS31YLR167WverifiedFusion protein that is cleaved to yield a ribosomal protein ofthe small (40S) subunit and ubiquitin;ubiquitin may facilitate assembly of theribosomal protein into ribosomes; interactsgenetically with translation factor eIF2B 0.074150.23302&radic&radic&radic&radic S000003472PFK1YGR240CverifiedAlpha subunit of heterooctameric phosphofructokinase involvedin glycolysis, indispensable for anaerobicgrowth, activated by fructose-2,6-bisphosphateand AMP, mutation inhibits glucose induction ofcell cycle-related genes 0.073980.23247&radic&radic&radic S000002995CWH41YGL027CverifiedProcessing alpha glucosidase I, ER type II integral membraneN-glycoprotein involved in assembly of cellwall beta 1,6 glucan and asparagine-linkedprotein glycosylation; also involved in ERprotein quality control and sensing of ERstress 0.073810.23191&radic&radic&radic&radic&radic S000003450CRM1YGR218WverifiedMajor karyopherin, involved in export of proteins, RNAs, andribosomal subunits from the nucleus 0.07360.23127&radic&radic S000000028SNC1YAL030WverifiedVesicle membrane receptor protein (v-SNARE) involved in thefusion between Golgi-derived secretory vesicleswith the plasma membrane; proposed to beinvolved in endocytosis; member of thesynaptobrevin/VAMP family of R-type v-SNAREproteins 0.073540.23118&radic&radic S000000440ABD1YBR236CverifiedMethyltransferase, catalyzes the transfer of a methyl groupfrom S-adenosylmethionine to the GpppN terminusof capped mRNA 0.073560.23118&radic&radic&radic&radic&radic S000003995SSL1YLR005WverifiedComponent of the core form of RNA polymerase transcriptionfactor TFIIH, which has both protein kinase andDNA-dependent ATPase/helicase activities and isessential for transcription and nucleotideexcision repair; interacts with Tfb4p 0.073480.23096&radic&radic&radic&radic S000006297ASR1YPR093CverifiedProtein involved in a putative alcohol-responsive signalingpathway; accumulates in the nucleus underalcohol stress; contains a Ring/PHD fingerdomain 0.073490.23096 S000000927RSP5YER125WverifiedUbiquitin-protein ligase involved in ubiquitin-mediated proteindegradation; plays a role in heat shock element(HSE)-mediated gene expression andmultivesicular body sorting; contains a hect(homologous to E6-AP carboxyl terminus) domain 0.073260.23057&radic S000004593TUB3YML124CverifiedAlpha-tubulin; associates with beta-tubulin (Tub2p) to formtubulin dimer, which polymerizes to formmicrotubules; expressed at lower level thanTub1p 0.073260.23049&radic&radic&radic S000005854SNC2YOR327CverifiedVesicle membrane receptor protein (v-SNARE) involved in thefusion between Golgi-derived secretory vesicleswith the plasma membrane; member of thesynaptobrevin/VAMP family of R-type v-SNAREproteins 0.072880.22962&radic&radic&radic S000003430YPP1YGR198WverifiedCargo-transport protein involved in endocytosis; may interactwith ribosomes, based on co-purificationexperiments; GFP-fusion protein localizes tothe cytoplasm; YGR198W is an essential gene 0.072870.22962&radic&radic&radic&radic S000005364SIN3YOL004WverifiedComponent of the Sin3p-Rpd3p histone deacetylase complex,involved in transcriptional repression andactivation of diverse processes, includingmating-type switching and meiosis; involved inthe maintenance of chromosomal integrity 0.072690.22907&radic S000001564TEF4YKL081WverifiedTranslation elongation factor EF-1 gamma 0.072620.22884&radic&radic&radic S000004968FAP1YNL023CverifiedProtein that binds to Fpr1p (FKBP12), conferring rapamycinresistance by competing with rapamycin forFpr1p binding; has similarity to putativetranscription factors, including D.melanogaster shuttle craft and human NFX1 0.072480.2284 S000004442HMG2YLR450WverifiedOne of two isozymes of HMG-CoA reductase that convert HMG-CoAto mevalonate, a rate-limiting step in sterolbiosynthesis; overproduction induces assemblyof peripheral ER membrane arrays and shortnuclear-associated membrane stacks 0.072120.22746&radic&radic&radic S000003062PAN2YGL094CverifiedEssential subunit of the Pan2p-Pan3p poly(A)-ribonucleasecomplex, which acts to control poly(A) taillength and regulate the stoichiometry andactivity of postreplication repair complexes 0.0720.22717&radic S000006134LEA1YPL213WverifiedComponent of U2 snRNP; disruption causes reduced U2 snRNPlevels; physically interacts with Msl1p;invovled in telomere maintenance; putativehomolog of human U2A' snRNP protein 0.071830.22678&radic&radic&radic&radic&radic&radic&radic S000001458MUC1YIR019CverifiedGPI-anchored cell surface glycoprotein required for diploidpseudohyphal formation and haploid invasivegrowth, transcriptionally regulated by the MAPKpathway (via Ste12p and Tec1p) and the cAMPpathway (via Flo8p) 0.071570.22595&radic&radic&radic S000003494BUD32YGR262CverifiedProtein kinase proposed to be involved in bud-site selection,telomere uncapping and elongation, andtranscription; component of the EKC/KEOPSprotein complex with Kae1p, Cgi121p, Pcc1p, andGon7p 0.07150.22583&radic&radic&radic S000004170SAM1YLR180WverifiedS-adenosylmethionine synthetase, catalyzes transfer of theadenosyl group of ATP to the sulfur atom ofmethionine; one of two differentially regulatedisozymes (Sam1p and Sam2p) 0.071150.22489&radic&radic&radic&radic&radic&radic S000006339CTF4YPR135WverifiedChromatin-associated protein, required for sister chromatidcohesion; interacts with DNA polymerase alpha(Pol1p) and may link DNA synthesis to sisterchromatid cohesion 0.071080.22471&radic&radic&radic&radic&radic&radic S000000950SPT15YER148WverifiedTATA-binding protein, general transcription factor thatinteracts with other factors to form thepreinitiation complex at promoters, essentialfor viability 0.070550.22324&radic S000006382PRP4YPR178WverifiedSplicing factor, component of the U4/U6-U5 snRNP complex 0.070520.22302&radic&radic&radic&radic&radic S000001680PEX1YKL197CverifiedAAA-family ATPase peroxin required for peroxisome biogenesis,contains two 230 amino acid ATP-binding AAAcassettes, upregulated in anaerobiosis; Pex1pand Pex6p interact via their N-terminalAAA-cassettes 0.070250.22228&radic&radic&radic S000002745MRPS28YDR337WverifiedMitochondrial ribosomal protein of the small subunit 0.069910.22141&radic&radic&radic S000005654ADE2YOR128CverifiedPhosphoribosylaminoimidazole carboxylase, catalyzes a step inthe 'de novo' purine nucleotide biosyntheticpathway; red pigment accumulates in mutantcells deprived of adenine 0.069640.22036&radic&radic&radic&radic&radic&radic&radic S000005272MDJ2YNL328CverifiedConstituent of the mitochondrial import motor associated withthe presequence translocase; function overlapswith that of Pam18p; stimulates the ATPaseactivity of Ssc1p to drive mitochondrialimport; contains a J domain 0.069610.22036&radic&radic S000004623MAC1YMR021CverifiedCopper-sensing transcription factor involved in regulation ofgenes required for high affinity coppertransport 0.069320.21979&radic&radic&radic&radic&radic S000000021PMT2YAL023CverifiedProtein O-mannosyltransferase, transfers mannose residues fromdolichyl phosphate-D-mannose to proteinserine/threonine residues; acts in a complexwith Pmt1p, can instead interact with Pmt5p insome conditions; target for new antifungals 0.069290.21971&radic&radic&radic S000004103HOG1YLR113WverifiedMitogen-activated protein kinase involved in osmoregulation viathree independent osmosensors; mediates therecruitment and activation of RNA Pol II atHot1p-dependent promoters; localizationregulated by Ptp2p and Ptp3p 0.069260.21963&radic&radic&radic&radic&radic&radic&radic S000003427SKI6YGR195Wverified3'-to-5' phosphorolytic exoribonuclease that is a subunit ofthe exosome; required for 3' processing of the5.8S rRNA; involved in 3' to 5' mRNAdegradation and translation inhibition ofnon-poly(A) mRNAs 0.068870.21843&radic&radic&radic&radic&radic S000004877CUE1YMR264WverifiedEndoplasmic reticulum membrane protein that recruits theubiquitin-conjugating enzyme Ubc7p to the ERwhere it functions in protein degradation;contains a CUE domain that binds ubiquitin tofacilitate intramolecular monoubiquitination &radic0.06880.21827&radic&radic&radic&radic&radic S000005105CBK1YNL161WverifiedSerine/threonine protein kinase that regulates cellmorphogenesis pathways; involved in cell wallbiosynthesis, apical growth, proper matingprojection morphology, bipolar bud siteselection in diploid cells, and cell separation 0.068790.21821&radic&radic&radic&radic S000005724BFR1YOR198CverifiedComponent of mRNP complexes associated with polyribosomes;implicated in secretion and nuclearsegregation; multicopy suppressor of BFA(Brefeldin A) sensitivity 0.068730.21805&radic&radic&radic S000000184TEL1YBL088CverifiedProtein kinase primarily involved in telomere lengthregulation; contributes to cell cyclecheckpoint control in response to DNA damage;functionally redundant with Mec1p; homolog ofhuman ataxia telangiectasia (ATM) gene 0.068650.21785 S000005502RRP40YOL142WverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.068630.21781&radic S000000339CKS1YBR135WverifiedSubunit of the Cdc28 protein kinase, required for mitoticproteolysis, may also be involved in theproteolysis of the G1 cyclins 0.068470.2174&radic&radic&radic S000005887PDE2YOR360CverifiedHigh-affinity cyclic AMP phosphodiesterase, component of thecAMP-dependent protein kinase signaling system,protects the cell from extracellular cAMP,contains readthrough motif surroundingtermination codon 0.068240.21679&radic&radic&radic&radic&radic S000005582NOB1YOR056CverifiedEssential nuclear protein involved in proteasome maturation andsynthesis of 40S ribosomal subunits; requiredfor cleavage of the 20S pre-rRNA to generatethe mature 18S rRNA 0.068020.21624&radic&radic&radic&radic S000001518UGP1YKL035WverifiedUDP-glucose pyrophosphorylase (UGPase), catalyses thereversible formation of UDP-Glc from glucose1-phosphate and UTP, involved in a wide varietyof metabolic pathways, expression modulated byPho85p through Pho4p 0.067990.21619&radic&radic&radic S000000002VPS8YAL002WverifiedMembrane-associated hydrophilic protein that interacts with thesmall GTPase, Vps21p, to facilitate solublevacuolar protein localization; required forlocalization and trafficking of the CPY sortingreceptor; contains a RING finger motif 0.067910.216&radic&radic&radic&radic S000002386HOYDL227CverifiedSite-specific endonuclease required for gene conversion at theMAT locus (homothallic switching) through thegeneration of a ds DNA break; expressionrestricted to mother cells in late G1 ascontrolled by Swi4p-Swi6p, Swi5p and Ash1p 0.067710.21536&radic S000004334FKS1YLR342WverifiedCatalytic subunit of 1,3-beta-D-glucan synthase, functionallyredundant with alternate catalytic subunitGsc2p; binds to regulatory subunit Rho1p;involved in cell wall synthesis andmaintenance; localizes to sites of cell wallremodeling 0.067720.21536&radic&radic S000002274NUP84YDL116WverifiedSubunit of the nuclear pore complex (NPC), forms a subcomplexwith Nup85p, Nup120p, Nup145p-C, Sec13p, andSeh1p that plays a role in nuclear mRNA exportand NPC biogenesis 0.067640.21518&radic S000001102VMA22YHR060WverifiedIntegral membrane protein that is required for vacuolarH+-ATPase (V-ATPase) function, although not anactual component of the V-ATPase complex;functions in the assembly of the V-ATPase;localized to the yeast endoplasmic reticulum(ER) 0.06760.21513&radic&radic&radic&radic S000006139SAR1YPL218WverifiedGTPase, GTP-binding protein of the ARF family, component ofCOPII coat of vesicles; required for transportvesicle formation during ER to Golgi proteintransport 0.067490.21469&radic&radic&radic&radic S000003011DST1YGL043WverifiedGeneral transcription elongation factor TFIIS, enables RNApolymerase II to read through blocks toelongation by stimulating cleavage of nascenttranscripts stalled at transcription arrestsites 0.067470.21469&radic&radic&radic&radic S000006363KRE6YPR159WverifiedProtein required for beta-1,6 glucan biosynthesis; putativebeta-glucan synthase; appears functionallyredundant with Skn1p 0.067430.21457&radic&radic&radic&radic&radic S000003043MPS2YGL075CverifiedEssential membrane protein localized at the nuclear envelopeand spindle pole body (SPB), required forinsertion of the newly duplicated SPB into thenuclear envelope; potentially phosphorylated byCdc28p 0.067380.21445&radic&radic&radic S000006050TAF14YPL129WverifiedSubunit (30 kDa) of TFIID, TFIIF, and SWI/SNF complexes,involved in RNA polymerase II transcriptioninitiation and in chromatin modification,contains a YEATS domain 0.067320.21429&radic&radic&radic&radic S000000019CCR4YAL021CverifiedComponent of the CCR4-NOT transcriptional complex, which isinvolved in regulation of gene expression;component of the major cytoplasmic deadenylase,which is involved in mRNA poly(A) tailshortening 0.067270.21411&radic&radic&radic&radic&radic&radic S000005505CTR9YOL145CverifiedComponent of the Paf1p complex, which is a large complex thatbinds to and modulates the activity of RNApolymerase II and is required for expression ofa subset of genes, including cyclin genes;contains TPR repeats 0.067170.21367&radic S000004472RAD33YML011CuncharacterizedProtein involved in nucleotide excision repair; greenfluorescent protein (GFP)-fusion proteinlocalizes to the nucleus 0.066780.21261&radic&radic&radic&radic&radic S000005098YCK2YNL154CverifiedPalmitoylated, plasma membrane-bound casein kinase I isoform;shares redundant functions with Yck1p inmorphogenesis, proper septin assembly,endocytic trafficking; provides an essentialfunction overlapping with that of Yck1p 0.066740.21253&radic&radic&radic&radic S000002986JAC1YGL018CverifiedSpecialized J-protein that functions with Hsp70 in Fe-S clusterbiogenesis in mitochondria, involved in ironmetabolism; contains a J domain typical toJ-type chaperones; localizes to themitochondrial matrix 0.066660.21227&radic&radic&radic S000002198NAT1YDL040CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.066580.21199&radic S000001083SRB2YHR041CverifiedGeneral transcription factor, subunit of the RNA polymerase IImediator complex; associates with corepolymerase subunits to form the RNA polymeraseII holoenzyme; involved in telomere maintenance 0.066460.21166&radic&radic&radic&radic S000002673PEX10YDR265WverifiedRING finger peroxisomal membrane peroxin required forperoxisomal matrix protein import, interactswith Pex12p, links ubiquitin-conjugating Pex4pto protein import machinery; mutations in humanhomolog cause a variety of peroxisomaldisorders 0.066230.21099&radic&radic&radic&radic S000005208PDR17YNL264CverifiedPhosphatidylinositol transfer protein (PITP), downregulatesPlb1p-mediated turnover of phosphatidylcholine,found in the cytosol and microsomes, homologousto Pdr16p, deletion affects phospholipidcomposition 0.066140.21086&radic&radic&radic S000003723SWE1YJL187CverifiedProtein kinase that regulates the G2/M transition by inhibitionof Cdc28p kinase activity; localizes to thenucleus and to the daughter side of themother-bud neck; homolog of S. pombe Wee1p;potential Cdc28p substrate 0.066110.21077&radic&radic S000002567SSY1YDR160WverifiedComponent of the SPS plasma membrane amino acid sensor system(Ssy1p-Ptr3p-Ssy5p), which senses externalamino acid concentration and transmitsintracellular signals that result in regulationof expression of amino acid permease genes 0.065960.21027&radic&radic&radic&radic S000002483RAD55YDR076WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad57p 0.065770.20976&radic&radic&radic&radic S000004684SEC14YMR079WverifiedPhosphatidylinositol/phosphatidylcholine transfer proteininvolved in coordinate regulation of PtdIns andPtdCho metabolism, products of which areregulators in Golgi to plasma membranetransport; functionally homologous to mammalianPITPs 0.065580.20933&radic&radic&radic S000003666URA2YJL130CverifiedBifunctional carbamoylphosphate synthetase (CPSase)-aspartatetranscarbamylase (ATCase), catalyzes the firsttwo enzymatic steps in the de novo biosynthesisof pyrimidines; both activities are subject tofeedback inhibition by UTP 0.065550.20924&radic&radic&radic S000006284TEF1YPR080WverifiedTranslational elongation factor EF-1 alpha; also encoded byTEF2; functions in the binding reaction ofaminoacyl-tRNA (AA-tRNA) to ribosomes 0.065550.20924&radic&radic S000002534ARO1YDR127WverifiedPentafunctional arom protein, catalyzes steps 2 through 6 inthe biosynthesis of chorismate, which is aprecursor to aromatic amino acids 0.065480.20899&radic&radic&radic&radic&radic S000006074RAD53YPL153CverifiedProtein kinase, required for cell-cycle arrest in response toDNA damage; activated by transautophosphorylation when interacting withhyperphosphorylated Rad9p; also interacts withARS1 and plays a role in initiation of DNAreplication 0.065490.20899&radic&radic&radic&radic S000006260TFB4YPR056WverifiedSubunit of TFIIH complex, involved in transcription initiation,similar to 34 kDa subunit of human TFIIH;interacts with Ssl1p 0.065260.20844&radic S000001210THP2YHR167WverifiedSubunit of the THO complex, which connects transcriptionelongation and mitotic recombination, and ofthe TREX complex, which is recruited toactivated genes and couples transcription tomRNA export; involved in telomere maintenance 0.06520.20822&radic&radic&radic&radic&radic&radic&radic S000001600SBA1YKL117WverifiedCo-chaperone that binds to and regulates Hsp90 familychaperones; important for pp60v-src activity inyeast; homologous to the mammalian p23 proteins 0.065020.20761&radic&radic&radic&radic&radic S000006324CLB5YPR120CverifiedB-type cyclin involved in DNA replication during S phase;activates Cdc28p to promote initiation of DNAsynthesis; functions in formation of mitoticspindles along with Clb3p and Clb4p; mostabundant during late G1 phase 0.064930.20737&radic&radic S000006265JID1YPR061CverifiedProbable Hsp40p co-chaperone, has a DnaJ-like domain andappears to be involved in ER-associateddegradation of misfolded proteins containing atightly folded cytoplasmic domain; inhibitsreplication of Brome mosaic virus in S.cerevisiae 0.064830.20711 S000001410RPL40AYIL148WverifiedFusion protein, identical to Rpl40Bp, that is cleaved to yieldubiquitin and a ribosomal protein of the large(60S) ribosomal subunit with similarity to ratL40; ubiquitin may facilitate assembly of theribosomal protein into ribosomes 0.064640.20662&radic&radic S000001656SNU114YKL173WverifiedGTPase component of U5 snRNP involved in mRNA splicing viaspliceosome; binds directly to U5 snRNA;proposed to be involved in conformationalchanges of the spliceosome; similarity toribosomal translocation factor EF-2 0.064540.2064&radic&radic&radic&radic&radic&radic S000001573CUE2YKL090WverifiedProtein of unknown function; has two CUE domains that bindubiquitin, which may facilitate intramolecularmonoubiquitination 0.064290.20549&radic&radic S000004885SCS7YMR272CverifiedSphingolipid alpha-hydroxylase, functions in thealpha-hydroxylation of sphingolipid-associatedvery long chain fatty acids, has bothcytochrome b5-like and hydroxylase/desaturasedomains, not essential for growth 0.064230.20525&radic&radic&radic&radic S000005862ALA1YOR335CverifiedCytoplasmic alanyl-tRNA synthetase, required for proteinsynthesis; point mutation (cdc64-1 allele)causes cell cycle arrest at G1; lethality ofnull mutation is functionally complemented byhuman homolog 0.064050.20485&radic&radic&radic&radic&radic&radic S000000939YER137CUncharacterizedHypothetical protein 0.06380.20424&radic S000000544GID7YCL039WverifiedProtein of unknown function, involved in proteasome-dependentcatabolite inactivation offructose-1,6-bisphosphatase; contains six WD40repeats; computational analysis suggests thatGid7p and Moh1p have similar functions 0.06360.20359&radic&radic&radic&radic&radic S000002506BMH2YDR099Wverified14-3-3 protein, minor isoform; binds proteins and DNA, involvedin regulation of many processes includingexocytosis and vesicle transport, Ras/MAPKsignaling during pseudohyphal development,rapamycin-sensitive signaling, and others 0.063520.20337&radic&radic&radic&radic&radic S000000004SSA1YAL005CverifiedATPase involved in protein folding and nuclear localizationsignal (NLS)-directed nuclear transport; memberof heat shock protein 70 (HSP70) family; formsa chaperone complex with Ydj1p; localized tothe nucleus, cytoplasm, and cell wall 0.06340.20299&radic&radic&radic&radic&radic S000001073RRM3YHR031CverifiedDNA helicase involved in rDNA replication and Ty1transposition; relieves replication fork pausesat telomeric regions; structurally andfunctionally related to Pif1p 0.063290.20269&radic&radic&radic&radic&radic S000002550SAN1YDR143CverifiedUbiquitin-protein ligase, involved in the proteasome-dependentdegradation of aberrant nuclear proteins; san1mutations suppress sir4, spt16, and cdc68mutations, suggesting a role in chromatinsilencing &radic0.063140.20201&radic&radic S000003078CUE3YGL110CuncharacterizedProtein of unknown function; has a CUE domain that bindsubiquitin, which may facilitate intramolecularmonoubiquitination 0.062890.20152&radic&radic&radic S000005863KRE5YOR336WverifiedProtein required for beta-1,6 glucan biosynthesis; mutationsresult in aberrant morphology and severe growthdefects 0.062840.2014 S000004075ARP6YLR085CverifiedActin-related protein that binds nucleosomes; a component ofthe SWR1 complex, which exchanges histonevariant H2AZ (Htz1p) for chromatin-boundhistone H2A 0.062410.20007&radic&radic&radic&radic&radic&radic S000005835SNU66YOR308CverifiedComponent of the U4/U6.U5 snRNP complex involved in pre-mRNAsplicing via spliceosome; has homology to humanSART-1 and to an S. pombe protein; snu66 nullmutation confers cold-sensitivity but is notlethal at normal growth temperatures 0.062430.20007&radic&radic&radic&radic S000002976PMA1YGL008CverifiedPlasma membrane H+-ATPase, pumps protons out of the cell; majorregulator of cytoplasmic pH and plasma membranepotential; part of the P2 subgroup ofcation-transporting ATPases 0.062370.19991&radic&radic S000006305SNT309YPR101WverifiedComponent of NineTeen complex (NTC) containing Prp19p involvedin mRNA splicing, interacts physically andgenetically with Prp19p 0.062360.19979&radic S000002972RPN14YGL004CuncharacterizedPutative non-ATPase subunit of the 19S regulatory particle ofthe 26S proteasome; localized to the cytoplasm &radic0.062290.19977&radic&radic S000001019RIM101YHL027WverifiedTranscriptional repressor involved in response to pH and incell wall construction; required for alkalinepH-stimulated haploid invasive growth andsporulation; activated by proteolyticprocessing; similar to A. nidulans PacC 0.062120.1992&radic&radic&radic&radic&radic&radic S000000964RAD4YER162CverifiedProtein that recognizes and binds damaged DNA (with Rad23p)during nucleotide excision repair; subunit ofNuclear Excision Repair Factor 2 (NEF2);homolog of human XPC protein 0.06210.19917&radic&radic S000006017PNG1YPL096WverifiedConserved peptide N-glycanase required for deglycosylation ofmisfolded glycoproteins duringproteasome-dependent degradation, localizes tothe cytoplasm and nucleus, interacts with theDNA repair protein Rad23p 0.062070.19907&radic&radic&radic&radic&radic S000003567MAD2YJL030WverifiedComponent of the spindle-assembly checkpoint complex, whichdelays the onset of anaphase in cells withdefects in mitotic spindle assembly; forms acomplex with Mad1p 0.062060.19901&radic&radic S000000005ERP2YAL007CverifiedProtein that forms a heterotrimeric complex with Erp1p, Emp24p,and Erv25p; member, along with Emp24p andErv25p, of the p24 family involved in ER toGolgi transport and localized to COPII-coatedvesicles 0.061980.19876&radic S000002347PPH22YDL188CverifiedCatalytic subunit of protein phosphatase 2A, functionallyredundant with Pph21p; methylated at Cterminus; forms alternate complexes withseveral regulatory subunits; involved in signaltransduction and regulation of mitosis 0.061960.19867&radic&radic&radic S000006155TFP3YPL234CverifiedVacuolar ATPase V0 domain subunit c', involved in protontransport activity; hydrophobic integralmembrane protein (proteolipid) containing fourtransmembrane segments; N and C termini are inthe vacuolar lumen 0.06180.19816&radic&radic&radic&radic S000000392NTC20YBR188CverifiedMember of a complex, including Prp19p, that binds to thespliceosome; required for pre-mRNA splicing 0.061720.19789&radic&radic&radic&radic S000003355PPT1YGR123CverifiedProtein serine/threonine phosphatase with similarity to humanphosphatase PP5; present in both the nucleusand cytoplasm; expressed during logarithmicgrowth 0.061610.19758&radic&radic&radic&radic&radic S000003213RTF1YGL244WverifiedSubunit of the RNA polymerase II-associated Paf1 complex;directly or indirectly regulates DNA-bindingproperties of Spt15p and relative activities ofdifferent TATA elements; involved in telomeremaintenance 0.061090.19599&radic&radic&radic&radic&radic&radic S000000870MOT2YER068WverifiedComponent of the CCR4-NOT complex, which has multiple roles intranscription regulation, mRNA degradation, andpost-transcriptional modifications; with Ubc4p,ubiquitylates subunits of the nascentpolypeptide-associated complex (NAC) 0.060980.19572&radic&radic&radic&radic S000004460YPT7YML001WverifiedGTPase; GTP-binding protein of the rab family; required forhomotypic fusion event in vacuole inheritance,for endosome-endosome fusion, similar tomammalian Rab7 0.060870.1954&radic&radic&radic&radic&radic&radic&radic S000004771HLJ1YMR161WverifiedCo-chaperone for Hsp40p, anchored in the ER membrane; with itshomolog Hdj1p promotes ER-associated proteindegradation (ERAD) of integral membranesubstrates; similar to E. coli DnaJ &radic0.060740.19487&radic&radic&radic S000004419MAG2YLR427WuncharacterizedDNA-3-methyladenine glycosidase II that catalyzes of thehydrolysis of alkylated DNA 0.060640.19474&radic&radic S000003279TFC4YGR047CverifiedOne of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauA domain of TFIIIC that binds BoxA DNApromoter sites of tRNA and similar genes; hasTPR motifs; human homolog is TFIIIC-102 0.060560.19455&radic&radic&radic&radic&radic&radic S000005448MPD2YOL088CverifiedMember of the protein disulfide isomerase (PDI) family,exhibits chaperone activity; overexpressionsuppresses the lethality of a pdi1 deletion butdoes not complement all Pdi1p functions;undergoes oxidation by Ero1p 0.060540.19448 S000000521DCC1YCL016CverifiedSubunit of a complex with Ctf8p and Ctf18p that shares somecomponents with Replication Factor C, requiredfor sister chromatid cohesion and telomerelength maintenance 0.060060.19315&radic&radic&radic&radic&radic S000003631BCK1YJL095WverifiedMitogen-activated protein (MAP) kinase kinase kinase acting inthe protein kinase C signaling pathway, whichcontrols cell integrity; upon activation byPkc1p phosphorylates downstream kinases Mkk1pand Mkk2p 0.059890.19264&radic S000000735GCN4YEL009CverifiedTranscriptional activator of amino acid biosynthetic genes inresponse to amino acid starvation; expressionis tightly regulated at both thetranscriptional and translational levels 0.059870.19264&radic&radic&radic&radic S000004760YIM1YMR152WverifiedProtein of unknown function; null mutant displays sensitivityto DNA damaging agents; the authentic,non-tagged protein is detected in highlypurified mitochondria in high-throughputstudies 0.059910.19264&radic&radic&radic&radic&radic S000001659LST4YKL176CverifiedProtein possibly involved in a post-Golgi secretory pathway;required for the transport ofnitrogen-regulated amino acid permease Gap1pfrom the Golgi to the cell surface 0.059540.19164&radic&radic&radic&radic S000003060NUP145YGL092WverifiedEssential nucleoporin, catalyzes its own cleavage in vivo togenerate a C-terminal fragment that assemblesinto the Nup84p subcomplex of the nuclear porecomplex, and an N-terminal fragment of unknownfunction that is homologous to Nup100p 0.059540.19164&radic S000003804POL32YJR043CverifiedThird subunit of DNA polymerase delta, involved in chromosomalDNA replication; required for error-prone DNAsynthesis in the presence of DNA damage andprocessivity; interacts with Hys2p, PCNA(Pol30p), and Pol1p 0.059190.19064&radic&radic S000006393SKI3YPR189WverifiedProtein involved in exosome mediated 3' to 5' mRNA degradationand translation inhibition of non-poly(A)mRNAs; forms complex with Ski2p and Ski8p;required for repressing propagation of dsRNAviruses 0.058720.18918&radic&radic S000001002YHL010CUncharacterizedPutative protein of unknown function, contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences 0.058530.18864&radic&radic&radic&radic S000004322CHS5YLR330WverifiedProtein involved in export from the Golgi to plasma membrane;involved in chitin biosynthesis through itsrole in Chs3p localization; interacts withArf1p, Bch1p, Fmp50p, Bud7p, and Chs6p 0.058530.18847&radic&radic&radic&radic S000002551MKC7YDR144CverifiedGPI-anchored aspartyl protease (yapsin) involved in proteinprocessing; shares functions with Yap3p andKex2p 0.058340.18795 S000001584HSL1YKL101WverifiedNim1p-related protein kinase that regulates the morphogenesisand septin checkpoints; associates with theassembled septin filament; required along withHsl7p for bud neck recruitment,phosphorylation, and degradation of Swe1p 0.058260.18779&radic&radic S000001159TOM71YHR117WverifiedMitochondrial outer membrane protein with similarity to Tom70p;probable minor component of the TOM(translocase of outer membrane) complexresponsible for recognition and import ofmitochondrially directed proteins 0.058160.18747&radic&radic&radic&radic S000004886ZDS1YMR273CverifiedProtein that interacts with silencing proteins at the telomere,involved in transcriptional silencing; has arole in localization of Bcy1p, a regulatorysubunit of protein kinase A; implicated in mRNAnuclear export 0.058110.18724&radic&radic&radic&radic&radic&radic S000006222RLF2YPR018WverifiedLargest subunit (p90) of the Chromatin Assembly Complex (CAF-I)with Cac2p and Msi1p that assembles newlysynthesized histones onto recently replicatedDNA; involved in the maintenance oftranscriptionally silent chromatin 0.058050.1872 S000005846HSH49YOR319WverifiedU2-snRNP associated splicing factor with similarity to themammalian splicing factor SAP49; proposed tofunction as a U2-snRNP assembly factor alongwith Hsh155p and binding partner Cus1p;contains two RNA recognition motifs (RRM) 0.058010.18706&radic&radic&radic&radic&radic S000001266BET1YIL004CverifiedType II membrane protein required for vesicular transportbetween the endoplasmic reticulum and Golgicomplex; v-SNARE with similarity tosynaptobrevins 0.057990.18706 S000003700TPK1YJL164CverifiedSubunit of cytoplasmic cAMP-dependent protein kinase, whichcontains redundant catalytic subunits Tpk1p,Tpk2p, and Tpk3p and regulatory subunit Bcy1p;promotes vegetative growth in response tonutrients; inhibits filamentous growth 0.057990.18706&radic&radic&radic S000001895DEG1YFL001WverifiedNon-essential tRNA:pseudouridine synthase, introducespseudouridines at position 38 or 39 in tRNA,important for maintenance of translationefficiency and normal cell growth, localizes toboth the nucleus and cytoplasm 0.057890.18653&radic S000003132YRB30YGL164CverifiedRanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTPhydrolysis of RanGTP (Gsp1p); shares similarityto proteins in other fungi but not in highereukaryotes 0.057830.18642&radic&radic S000001540NUP120YKL057CverifiedSubunit of the Nup84p subcomplex of the nuclear pore complex(NPC), required for even distribution of NPCsaround the nuclear envelope, involved inestablishment of a normal nucleocytoplasmicconcentration gradient of the GTPase Gsp1p 0.057580.18575&radic&radic S000000331VMA2YBR127CverifiedSubunit B of the eight-subunit V1 peripheral membrane domain ofthe vacuolar H+-ATPase (V-ATPase), anelectrogenic proton pump found throughout theendomembrane system; contains nucleotidebinding sites; also detected in the cytoplasm 0.057570.1856&radic S000003959PRP19YLL036CverifiedSplicing factor associated with the spliceosome; contains aU-box, a motif found in a class of ubiquitinligases &radic0.05730.18483&radic&radic&radic&radic S000000038CLN3YAL040CverifiedG1 cyclin involved in cell cycle progression; activates Cdc28pkinase to promote the G1 to S phase transition;plays a role in regulating transcription of theother G1 cyclins, CLN1 and CLN2; regulated byphosphorylation and proteolysis 0.057260.18468&radic&radic&radic&radic&radic S000000959COG3YER157WverifiedEssential component of the conserved oligomeric Golgi complex(Cog1p through Cog8p), a cytosolic tetheringcomplex that functions in protein traffickingto mediate fusion of transport vesicles toGolgi compartments 0.0570.18385 S000005162MGS1YNL218WverifiedProtein with DNA-dependent ATPase and ssDNA annealingactivities involved in maintenance of genome;interacts functionally with DNA polymerasedelta; homolog of human Werner helicaseinteracting protein (WHIP) 0.056780.1832&radic&radic&radic&radic&radic S000005294PRP2YNR011CverifiedRNA-dependent ATPase in the DEAH-box family, required foractivation of the spliceosome before the firsttransesterification step in RNA splicing 0.056760.18319&radic&radic S000004312MMS22YLR320WverifiedProtein involved in resistance to ionizing radiation; acts withMms1p in a repair pathway that may be involvedin resolving replication intermediates orpreventing the damage caused by blockedreplication forks 0.056730.18306&radic&radic S000002536SAC6YDR129CverifiedFimbrin, actin-bundling protein; cooperates with Scp1p(calponin/transgelin) in the organization andmaintenance of the actin cytoskeleton 0.056690.18298&radic&radic&radic&radic S000004391BDF1YLR399CverifiedProtein involved in transcription initiation at TATA-containingpromoters; associates with the basaltranscription factor TFIID; contains twobromodomains; corresponds to the C-terminalregion of mammalian TAF1; redundant with Bdf2p 0.056650.18286&radic&radic&radic&radic&radic S000004836UBP8YMR223WverifiedUbiquitin-specific protease that is a component of the SAGA(Spt-Ada-Gcn5-Acetyltransferase) acetylationcomplex; required for SAGA-mediateddeubiquitination of histone H2B 0.056480.18242&radic&radic&radic&radic&radic S000006367TIF3YPR163CverifiedTranslation initiation factor eIF-4B, has RNA annealingactivity; contains an RNA recognition motif andbinds to single-stranded RNA 0.056460.18229&radic&radic&radic&radic&radic&radic S000003957RIX7YLL034CverifiedPutative ATPase of the AAA family, required for export ofpre-ribosomal large subunits from the nucleus;distributed between the nucleolus, nucleoplasm,and nuclear periphery depending on growthconditions 0.056380.18225&radic&radic&radic&radic S000000433ROT2YBR229CverifiedGlucosidase II catalytic subunit required for normal cell wallsynthesis; mutations in rot2 suppress tor2mutations, and are synthetically lethal withrot1 mutations 0.056210.18174&radic&radic&radic&radic S000004172SWI6YLR182WverifiedTranscription cofactor, forms complexes with DNA-bindingproteins Swi4p and Mbp1p to regulatetranscription at the G1/S transition; involvedin meiotic gene expression; localizationregulated by phosphorylation; potential Cdc28psubstrate 0.056130.18156&radic&radic&radic&radic&radic&radic S000002351ARF1YDL192WverifiedADP-ribosylation factor, GTPase of the Ras superfamily involvedin regulation of coated formation vesicles inintracellular trafficking within the Golgi;functionally interchangeable with Arf2p 0.056120.1815&radic&radic&radic&radic&radic S000004473ERV25YML012WverifiedProtein that forms a heterotrimeric complex with Erp1, Erp2p,and Emp24, member of the p24 family involved inendoplasmic reticulum to Golgi transport 0.056090.18138&radic&radic&radic&radic S000004493NDC1YML031WverifiedNuclear envelope protein with multiple putative transmembranedomains, required for nuclear pore complexassembly and spindle pole body duplication;required for meiosis II 0.055990.18111 S000001868STE2YFL026WverifiedReceptor for alpha-factor pheromone; seven transmembrane-domainGPCR that interacts with both pheromone and aheterotrimeric G protein to initiate thesignaling response that leads to mating betweenhaploid a and alpha cells 0.055820.18055&radic&radic&radic&radic S000001377NUP159YIL115CverifiedSubunit of the nuclear pore complex that is found exclusivelyon the cytoplasmic side, forms a subcomplexwith Nup82p and Nsp1p, required for mRNA export 0.055770.18031&radic S000001404CCT2YIL142WverifiedSubunit beta of the cytosolic chaperonin Cct ring complex,related to Tcp1p, required for the assembly ofactin and tubulins in vivo 0.055780.18031&radic S000003858JJJ3YJR097WverifiedProtein of unknown function, contains a J-domain, which is aregion with homology to the E. coli DnaJprotein 0.055690.18019&radic&radic S000005667ARP8YOR141CverifiedNuclear actin-related protein involved in chromatin remodeling,component of chromatin-remodeling enzymecomplexes 0.055640.17995&radic&radic&radic&radic&radic&radic&radic S000004282GCD7YLR291CverifiedBeta subunit of the translation initiation factor eIF2B, theguanine-nucleotide exchange factor for eIF2;activity subsequently regulated byphosphorylated eIF2; first identified as anegative regulator of GCN4 expression 0.055520.17959&radic&radic&radic&radic&radic&radic S000005527RRP6YOR001WverifiedExonuclease component of the nuclear exosome; contributes tothe quality-control system that retains anddegrades aberrant mRNAs in the nucleus &radic0.055520.17959&radic&radic&radic&radic S000000018ATS1YAL020CverifiedProtein required, with Elongator complex, Kti11p, and Kti12p,for modification of wobble nucleosides in tRNA;has a potential role in regulatory interactionsbetween microtubules and the cell cycle 0.055440.17938&radic&radic&radic&radic&radic&radic&radic S000001441MPH1YIR002CverifiedMember of the DEAH family of helicases, functions in anerror-free DNA damage bypass pathway thatinvolves homologous recombination, mutationsconfer a mutator phenotype 0.054840.17775&radic&radic S000002793EFT2YDR385WverifiedElongation factor 2 (EF-2), also encoded by EFT1; catalyzesribosomal translocation during proteinsynthesis; contains diphthamide, the uniqueposttranslationally modified histidine residuespecifically ADP-ribosylated by diphtheriatoxin 0.054780.1776&radic&radic&radic&radic&radic&radic S000004853CUS1YMR240CverifiedProtein required for assembly of U2 snRNP into the spliceosome,forms a complex with Hsh49p and Hsh155p 0.054530.17697&radic&radic&radic&radic&radic S000003337VMA21YGR105WverifiedIntegral membrane protein that is required for vacuolarH+-ATPase (V-ATPase) function, although not anactual component of the V-ATPase complex;functions in the assembly of the V-ATPase;localized to the yeast endoplasmic reticulum(ER) 0.054410.17666&radic&radic&radic&radic&radic S000003803NUP85YJR042WverifiedSubunit of the Nup84p subcomplex of the nuclear pore complex(NPC), required for assembly of the subcomplexand also for formation of the nucleocytoplasmicGsp1p concentration gradient that plays a rolein nuclear trafficking 0.054290.17631 S000002892VPS52YDR484WverifiedComponent of the GARP (Golgi-associated retrograde protein)complex, Vps51p-Vps52p-Vps53p-Vps54p, which isrequired for the recycling of proteins fromendosomes to the late Golgi; involved inlocalization of actin and chitin 0.054260.17621&radic S000002906SEC20YDR498CverifiedMembrane glycoprotein v-SNARE involved in retrograde transportfrom the Golgi to the ER; required for N- andO-glycosylation in the Golgi but not in the ER;forms a complex with the cytosolic Tip20p 0.054230.17618 S000000054CNE1YAL058WverifiedCalnexin; integral membrane ER chaperone involved in foldingand quality control of glycoproteins; chaperoneactivity is inhibited by Mpd1p, with whichCne1p interacts; 24% identical to mammaliancalnexin; Ca+ binding not yet shown in yeast &radic0.05420.1761&radic&radic&radic S000006189PLC1YPL268WverifiedPhosphoinositide-specific phospholipase C, hydrolyzesphosphatidylinositol 4,5-biphosphate (PIP2) togenerate inositol 1,4,5-triphosphate (IP3) and1,2-diacylglycerol (DAG); involved inkinetochore function and pseudohyphaldifferentiation 0.05410.17585&radic S000000898SHC1YER096WverifiedSporulation-specific activator of Chs3p (chitin synthase III),required for the synthesis of the chitosanlayer of ascospores; has similarity to Skt5p,which activates Chs3p during vegetative growth;transcriptionally induced at alkaline pH 0.053970.17548 S000003158CDC55YGL190CverifiedNon-essential regulatory subunit B of protein phosphatase 2A;has multiple roles in mitosis and proteinbiosynthesis; found in the nucleus of mostcells but also at the bud neck (large-buddedcells) and at the bud tip (small-budded cells) 0.053860.17518&radic S000005738STE4YOR212WverifiedG protein beta subunit, forms a dimer with Ste18p to activatethe mating signaling pathway, forms aheterotrimer with Gpa1p and Ste18p to dampensignaling; may recruit Rho1p to the polarizedgrowth site during mating; contains WD40repeats 0.053830.17508&radic&radic&radic&radic&radic&radic S000002577SEC7YDR170CverifiedGuanine nucleotide exchange factor (GEF) for ADP ribosylationfactors involved in proliferation of the Golgi,intra-Golgi transport and ER-to-Golgitransport; found in the cytoplasm and onGolgi-associated coated vesicles 0.053750.17486&radic&radic&radic&radic&radic S000000815PRP22YER013WverifiedDEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase,associates with lariat intermediates before thesecond catalytic step of splicing; mediatesATP-dependent mRNA release from the spliceosomeand unwinds RNA duplexes 0.053420.17391&radic&radic&radic S000004920UBP15YMR304WverifiedUbiquitin-specific protease that may play a role in ubiquitinprecursor processing 0.053390.17371&radic&radic&radic S000004034PDC1YLR044CverifiedMajor of three pyruvate decarboxylase isozymes, key enzyme inalcoholic fermentation, decarboxylates pyruvateto acetaldehyde; subject to glucose-, ethanol-,and autoregulation; involved in amino acidcatabolism 0.053320.17363&radic&radic&radic&radic&radic&radic S000001649TPK3YKL166CverifiedSubunit of cAMP-dependent protein kinase, which contains Tpk1p,Tpk2p, Tpk3p, and regulatory subunit Bcy1p;promotes growth in response to nutrients;mediates induction of apoptosis due toactin-mediated hyperactivation of Ras signaling 0.05330.17348&radic&radic&radic&radic S000004365VID22YLR373CverifiedGlycosylated integral membrane protein localized to the plasmamembrane; plays a role infructose-1,6-bisphosphatase (FBPase)degradation; involved in FBPase transport fromthe cytosol to Vid (vacuole import anddegradation) vesicles 0.053260.17335&radic&radic&radic&radic&radic S000001055ARD1YHR013CverifiedSubunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p,Nat5p); N-terminally acetylates many proteins,which influences multiple processes such as thecell cycle, heat-shock resistance, mating,sporulation, and telomeric silencing 0.053250.17335&radic&radic&radic&radic&radic S000003389CHO2YGR157WverifiedPhosphatidylethanolamine methyltransferase (PEMT), catalyzesthe first step in the conversion ofphosphatidylethanolamine to phosphatidylcholineduring the methylation pathway ofphosphatidylcholine biosynthesis 0.053240.17335&radic&radic&radic&radic S000004067FMP25YLR077WuncharacterizedPutative protein of unknown function; the authentic, non-taggedprotein is detected in highly purifiedmitochondria in high-throughput studies 0.053190.17313&radic&radic&radic&radic&radic S000002764SPC110YDR356WverifiedInner plaque spindle pole body (SPB) component, ortholog ofhuman kendrin; involved in connecting nuclearmicrotubules to SPB; interacts withTub4p-complex and calmodulin; phosphorylated byMps1p in cell cycle-dependent manner 0.053080.17294&radic&radic&radic&radic S000004388VPS33YLR396CverifiedATP-binding protein that is a subunit of the homotypic vacuolefusion and vacuole protein sorting (HOPS)complex; essential for membrane docking andfusion at both the Golgi-to-endosome andendosome-to-vacuole stages of protein transport 0.053010.17266&radic&radic&radic S000006249MNI2YPR045CuncharacterizedDeletion mutant is synthetically lethal with MEN mutants 0.052890.17237&radic S000003996SSK1YLR006CverifiedCytoplasmic response regulator, part of a two-component signaltransducer that mediates osmosensing via aphosphorelay mechanism; dephosphorylated formis degraded by the ubiquitin-proteasome system;potential Cdc28p substrate 0.052840.17216&radic&radic&radic&radic&radic S000004620YMR018WUncharacterizedPutative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene 0.052830.17216&radic&radic&radic S000004258SEC22YLR268WverifiedR-SNARE protein; assembles into SNARE complex with Bet1p, Bos1pand Sed5p; cycles between the ER and Golgicomplex; involved in anterograde and retrogradetransport between the ER and Golgi;synaptobrevin homolog 0.052820.17216&radic&radic&radic&radic&radic&radic S000001289KRE27YIL027CverifiedProtein of unknown function; null mutant shows K1 killer toxinresistance 0.052840.17216&radic&radic&radic S000003310PAC10YGR078CverifiedPart of the heteromeric co-chaperone GimC/prefoldin complex,which promotes efficient protein folding 0.052840.17216&radic&radic&radic&radic&radic S000003336SRB5YGR104CverifiedSubunit of the RNA polymerase II mediator complex; associateswith core polymerase subunits to form the RNApolymerase II holoenzyme; essential fortranscriptional regulation; involved intelomere maintenance 0.052610.17144&radic&radic&radic&radic&radic&radic S000003390MTR3YGR158Cverified3'5' exoribonuclease, exosome subunit; nucleolar proteininvolved in export of mRNA and ribosomalsubunits; homologous to the E. coli exonucleaseRNase PH 0.052350.17066&radic&radic&radic&radic&radic&radic S000005056DBP2YNL112WverifiedEssential ATP-dependent RNA helicase of the DEAD-box proteinfamily, involved in nonsense-mediated mRNAdecay and rRNA processing 0.052180.17005&radic&radic S000006211REC8YPR007CverifiedMeiosis-specific component of sister chromatid cohesioncomplex; maintains cohesion between sisterchromatids during meiosis I; maintains cohesionbetween centromeres of sister chromatids untilmeiosis II; homolog of S. pombe Rec8p 0.052180.17005 S000006090MEX67YPL169CverifiedPoly(A)RNA binding protein involved in nuclear mRNA export,component of the nuclear pore; ortholog ofhuman TAP 0.052090.16985&radic&radic&radic S000004665RNA14YMR061WverifiedCleavage and polyadenylation factor I (CF I) component involvedin cleavage and polyadenylation of mRNA 3'ends; bridges interaction between Rna15p andHrp1p in the CF I complex 0.051960.16958&radic S000000935GLC7YER133WverifiedCatalytic subunit of type 1 serine/threonine proteinphosphatase, involved in many processesincluding glycogen metabolism, sporulation, andmitosis; interacts with multiple regulatorysubunits; predominantly isolated with Sds22p 0.051910.16943&radic&radic S000001249SKN7YHR206WverifiedNuclear response regulator and transcription factor, part of abranched two-component signaling system;required for optimal induction of heat-shockgenes in response to oxidative stress; involvedin osmoregulation 0.051790.16911&radic&radic&radic&radic S000000787CIN8YEL061CverifiedKinesin motor protein involved in mitotic spindle assembly andchromosome segregation 0.051730.16887&radic&radic&radic&radic S000003202SEC15YGL233WverifiedEssential 113kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which mediates polarized targeting ofvesicles to active sites of exocytosis; Sec15passociates with Sec4p and vesicles 0.05160.16853&radic&radic&radic&radic S000000372PEX32YBR168WverifiedPeroxisomal integral membrane protein, involved in negativeregulation of peroxisome size; partiallyfunctionally redundant with Pex31p; geneticinteractions suggest action at a stepdownstream of steps mediated by Pex28p andPex29p 0.051580.16849 S000005180SIN4YNL236WverifiedSubunit of the Mediator complex; interacts with the RNApolymerase II holoenzyme to postively ornegatively regulate transcription; dispensiblefor basal transcription 0.051560.16846&radic&radic&radic S000003327RRP46YGR095CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex 0.051540.16842&radic&radic&radic&radic&radic S000006345KAR3YPR141CverifiedMinus-end-directed microtubule motor that functions in mitosisand meiosis, localizes to the spindle pole bodyand localization is dependent on functionalCik1p, required for nuclear fusion duringmating; potential Cdc28p substrate 0.051480.16812&radic&radic&radic&radic S000005599SGO1YOR073WverifiedComponent of the spindle checkpoint, involved in sensing lackof tension on mitotic chromosomes; protectscentromeric Rec8p at meiosis I; required foraccurate chromosomal segregation at meiosis IIand for mitotic chromosome stability 0.051440.16801&radic&radic&radic&radic S000003836HOC1YJR075WverifiedAlpha-1,6-mannosyltransferase involved in cell wall mannanbiosynthesis; subunit of a Golgi-localizedcomplex that also contains Anp1p, Mnn9p,Mnn11p, and Mnn10p; identified as a suppressorof a cell lysis sensitive pkc1-371 allele 0.051430.16801&radic S000004445RIF2YLR453CverifiedProtein that binds to the Rap1p C-terminus and actssynergistically with Rif1p to help controltelomere length and establish telomericsilencing; deletion results in telomereelongation 0.051220.16715&radic&radic S000000211DSF2YBR007CuncharacterizedDeletion suppressor of mpt5 mutation 0.051150.16702 S000003516ERV29YGR284CverifiedProtein localized to COPII-coated vesicles, involved in vesicleformation and incorporation of specificsecretory cargo 0.051120.16678&radic&radic&radic&radic S000000040ERV46YAL042WverifiedProtein localized to COPII-coated vesicles, forms a complexwith Erv41p; involved in the membrane fusionstage of transport 0.050990.16649&radic&radic S000002388SSB1YDL229WverifiedCytoplasmic ATPase that is a ribosome-associated molecularchaperone, functions with J-protein partnerZuo1p; may be involved in folding of newly-madepolypeptide chains; member of the HSP70 family;interacts with phosphatase subunit Reg1p 0.050940.16612&radic&radic S000005670ELG1YOR144CverifiedProtein required for S phase progression and telomerehomeostasis, forms an alternative replicationfactor C complex important for DNA replicationand genome integrity; involved in homologousrecombination-mediated DNA repair 0.05090.16612&radic&radic&radic&radic&radic&radic S000001049ERG11YHR007CverifiedLanosterol 14-alpha-demethylase, catalyzes the C-14demethylation of lanosterol to form4,4''-dimethylcholesta-8,14,24-triene-3-beta-ol in theergosterol biosynthesis pathway; member of thecytochrome P450 family 0.050970.16612&radic&radic&radic S000005273PEX6YNL329CverifiedPeroxisomal membrane AAA-family ATPase peroxin required forperoxisome assembly, contains two 230 aminoacid ATP-binding AAA cassettes, interacts withPex1p 0.050550.16529&radic&radic S000000483PAF1YBR279WverifiedRNA polymerase II-associated protein, defines a large complexthat is biochemically and functionally distinctfrom the Srb-Mediator form of Pol II holoenzymeand is required for full expression of a subsetof cell cycle-regulated genes 0.05050.16514&radic&radic S000002314CLB3YDL155WverifiedB-type cyclin involved in cell cycle progression; activatesCdc28p to promote the G2/M transition; may beinvolved in DNA replication and spindleassembly; accumulates during S phase and G2,then targeted for ubiquitin-mediateddegradation 0.050370.16461&radic&radic S000003956IRC19YLL033WuncharacterizedPutative protein of unknown function; YLL033W is not anessential gene but mutant is defective in sporeformation; null mutant displays increasedlevels of spontaneous Rad52 foci 0.050240.1644&radic&radic&radic&radic&radic S000000011DEP1YAL013WverifiedTranscriptional modulator involved in regulation of structuralphospholipid biosynthesis genes andmetabolically unrelated genes, as well asmaintenance of telomeres, mating efficiency,and sporulation 0.050230.16428&radic&radic&radic&radic S000001503SPT23YKL020CverifiedER membrane protein involved in regulation of OLE1transcription, acts with homolog Mga2p;inactive ER form dimerizes and one subunit isthen activated byubiquitin/proteasome-dependent processingfollowed by nuclear targeting 0.050220.16428&radic&radic&radic&radic S000004925PSE1YMR308CverifiedKaryopherin/importin that interacts with the nuclear porecomplex; acts as the nuclear import receptorfor specific proteins, including Pdr1p, Yap1p,Ste12p, and Aft1p 0.050220.16428&radic&radic S000000283RPG1YBR079CverifiedSubunit of the core complex of translation initiation factor3(eIF3), essential for translation; part of asubcomplex (Prt1p-Rpg1p-Nip1p) that stimulatesbinding of mRNA and tRNA(i)Met to ribosomes 0.050010.16378&radic&radic S000005335POP2YNR052CverifiedRNase of the DEDD superfamily, subunit of the Ccr4-Not complexthat mediates 3' to 5' mRNA deadenylation 0.049880.16332&radic&radic&radic S000004876SAP30YMR263WverifiedSubunit of a histone deacetylase complex, along with Rpd3p andSin3p, that is involved in silencing attelomeres, rDNA, and silent mating-type loci;involved in telomere maintenance 0.049710.16278&radic&radic&radic&radic&radic S000000369UBS1YBR165WverifiedUbiquitin-conjugating enzyme suppressor that functions as ageneral positive regulator of Cdc34p activity;nuclear protein that may represent a linkbetween nucleocytoplasmic transport andubiquitin ligase activity 0.049580.16237 S000002926EUG1YDR518WverifiedProtein disulfide isomerase of the endoplasmic reticulum lumen,function overlaps with that of Pdi1p; mayinteract with nascent polypeptides in the ER 0.049560.16225&radic S000003539OST1YJL002CverifiedAlpha subunit of the oligosaccharyltransferase complex of theER lumen, which catalyzes asparagine-linkedglycosylation of newly synthesized proteins 0.049490.16197&radic S000006228YME1YPR024WverifiedSubunit, with Mgr1p, of the mitochondrial inner membrane i-AAAprotease complex, which is responsible fordegradation of unfolded or misfoldedmitochondrial gene products; mutation causes anelevated rate of mitochondrial turnover 0.049480.16197 S000005816SNF2YOR290CverifiedCatalytic subunit of the SWI/SNF chromatin remodeling complexinvolved in transcriptional regulation;contains DNA-stimulated ATPase activity;functions interdependently in transcriptionalactivation with Snf5p and Snf6p 0.049440.16187&radic&radic&radic&radic S000000065RFA1YAR007CverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 0.049430.16187&radic&radic&radic S000003289LST7YGR057CverifiedProtein possibly involved in a post-Golgi secretory pathway;required for the transport ofnitrogen-regulated amino acid permease Gap1pfrom the Golgi to the cell surface 0.049220.16111&radic&radic&radic&radic S000001686TOR2YKL203CverifiedPIK-related protein kinase and rapamycin target; subunit ofTORC1, a complex that regulates growth inresponse to nutrients and TORC2, a complex thatregulates cell-cycle dependent polarization ofthe actin cytoskeleton; involved in meiosis 0.049180.16103&radic S000005976LGE1YPL055CverifiedProtein of unknown function; null mutant forms abnormally largecells 0.049090.16079&radic&radic&radic&radic&radic&radic S000003704SET2YJL168CverifiedHistone methyltransferase with a role in transcriptionalelongation, methylates a lysine residue ofhistone H3; associates with the C-terminaldomain of Rpo21p; histone methylation activityis regulated by phosphorylation status ofRpo21p 0.049060.16069&radic&radic&radic&radic S000001856YPT1YFL038CverifiedRas-like small GTPase, involved in the ER-to-Golgi step of thesecretory pathway; complex formation with theRab escort protein Mrs6p is required forprenylation of Ypt1p by proteingeranylgeranyltransferase type II (Bet2p-Bet4p) 0.048990.1605&radic&radic&radic S000000513YCL007CDubiousDubious ORF unlikely to encode a protein; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A 0.048990.1605&radic S000000728WBP1YEL002CverifiedBeta subunit of the oligosaccharyl transferase (OST)glycoprotein complex; required for N-linkedglycosylation of proteins in the endoplasmicreticulum 0.048970.16037 S000002674YDR266CUncharacterizedHypothetical protein 0.048980.16037&radic&radic&radic&radic&radic&radic S000000266YBR062CUncharacterizedHypothetical protein 0.048760.15976 S000005533SGT2YOR007CverifiedGlutamine-rich cytoplasmic protein of unknown function;contains tetratricopeptide (TPR) repeats, whichoften mediate protein-protein interactions; hassimilarity to human SGT, which is a cochaperonethat negatively regulates Hsp70 0.048730.1597&radic&radic&radic&radic&radic&radic&radic S000004252YPT6YLR262CverifiedGTPase, Ras-like GTP binding protein involved in the secretorypathway, required for fusion ofendosome-derived vesicles with the late Golgi,maturation of the vacuolar carboxypeptidase Y;has similarity to the human GTPase, Rab6 0.048740.1597&radic&radic&radic&radic&radic S000005195NRD1YNL251CverifiedRNA-binding protein that interacts with the C-terminal domainof the RNA polymerase II large subunit(Rpo21p), required for transcriptiontermination and 3' end maturation ofnonpolyadenylated RNAs 0.048720.15966&radic&radic S000005831ISW2YOR304WverifiedMember of the imitation-switch (ISWI) class of ATP-dependentchromatin remodeling complexes; ATPasecomponent that, with Itc1p, forms a complexrequired for repression of a-specific genes,INO1, and early meiotic genes during mitoticgrowth 0.048620.15937&radic&radic&radic S000002722RAD34YDR314CverifiedProtein involved in nucleotide excision repair (NER);homologous to RAD4 0.048420.15864&radic&radic S000002269RRP42YDL111CverifiedProtein involved in rRNA processing; component of the exosome3->5 exonuclease complex with Rrp4p, Rrp41p,Rrp43p and Dis3p 0.048350.15845&radic&radic S000004540HMG1YML075CverifiedOne of two isozymes of HMG-CoA reductase that catalyzes theconversion of HMG-CoA to mevalonate, which is arate-limiting step in sterol biosynthesis;localizes to the nuclear envelope;overproduction induces the formation ofkarmellae 0.048230.15797 S000006391RPO26YPR187WverifiedRNA polymerase subunit ABC23, common to RNA polymerases I, II,and III; part of central core; similar tobacterial omega subunit 0.048170.15794&radic&radic S000004265SMD2YLR275WverifiedCore Sm protein Sm D2; part of heteroheptameric complex (withSmb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D2 0.048140.15783&radic&radic&radic&radic&radic&radic S000005730DED1YOR204WverifiedATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, requiredfor translation initiation of all yeast mRNAs;mutations in human DEAD-box DBY are a frequentcause of male infertility 0.048090.15768&radic&radic S000005143GCR2YNL199CverifiedTranscriptional activator of genes involved in glycolysis;interacts and functions with the DNA-bindingprotein Gcr1p 0.048050.15754&radic&radic&radic&radic S000000539LSB5YCL034WverifiedProtein of unknown function; binds Las17p, which is a homologof human Wiskott-Aldrich Syndrome proteininvolved in actin patch assembly and actinpolymerization 0.047920.15714&radic&radic&radic S000002772CDC40YDR364CverifiedPre-mRNA splicing factor, important for catalytic step II ofpre-mRNA splicing and plays a role in cellcycle progression; required for DNA synthesisduring mitosis and meiosis; has WD repeats 0.047920.1571&radic&radic&radic&radic&radic&radic&radic S000005593ALG8YOR067CverifiedGlucosyl transferase, involved in N-linked glycosylation; addsglucose to the dolichol-linked oligosaccharideprecursor prior to transfer to protein duringlipid-linked oligosaccharide biosynthesis;similar to Alg6p 0.047920.1571&radic&radic&radic&radic&radic S000004246HAP1YLR256WverifiedZinc finger transcription factor involved in the complexregulation of gene expression in response tolevels of heme and oxygen; the S288C sequencediffers from other strain backgrounds due to aTy1 insertion in the carboxy terminus 0.047890.15692&radic S000003490RAD2YGR258CverifiedSingle-stranded DNA endonuclease, cleaves single-stranded DNAduring nucleotide excision repair to excisedamaged DNA; subunit of Nucleotide ExcisionRepair Factor 3 (NEF3); homolog of human XPGprotein 0.047770.15661&radic&radic S000004137SMD3YLR147CverifiedCore Sm protein Sm D3; part of heteroheptameric complex (withSmb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D3 0.047760.15648&radic&radic&radic S000002586CSN9YDR179CverifiedSubunit of the Cop9 signalosome, which is required fordeneddylation, or removal of the ubiquitin-likeprotein Rub1p from Cdc53p (cullin); involved inadaptation to pheromone signaling 0.047630.15608&radic&radic&radic&radic&radic S000006259SEC8YPR055WverifiedEssential 121kDa subunit of the exocyst complex (Sec3p, Sec5p,Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, andExo84p), which has the essential function ofmediating polarized targeting of secretoryvesicles to active sites of exocytosis 0.047580.15597&radic&radic S000001511TFA1YKL028WverifiedTFIIE large subunit, involved in recruitment of RNA polymeraseII to the promoter, activation of TFIIH, andpromoter opening 0.047570.15593&radic&radic&radic S000002188PRP9YDL030WverifiedSubunit of the SF3a splicing factor complex, required forspliceosome assembly; acts after the formationof the U1 snRNP-pre-mRNA complex 0.047520.15576&radic S000004826CEF1YMR213WverifiedEssential splicing factor; associated with Prp19p and thespliceosome, contains an N-terminal c-Myb DNAbinding motif necessary for cell viability butnot for Prp19p association, evolutionarilyconserved and homologous to S. pombe Cdc5p 0.04740.15502&radic&radic&radic&radic S000000120NCL1YBL024WverifiedS-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase,methylates cytosine to m5C at several positionsin tRNAs and intron-containing pre-tRNAs;similar to Nop2p and human proliferationassociated nucleolar protein p120 0.047360.15502&radic&radic S000003361SYF2YGR129WverifiedComponent of the spliceosome complex involved in pre-mRNAsplicing; involved in regulation of cell cycleprogression 0.04740.15502&radic&radic&radic S000000889YER087WUncharacterizedHypothetical protein 0.047320.15502&radic&radic&radic S000003052GUP1YGL084CverifiedPlasma membrane protein with a possible role in proton symportof glycerol; member of the MBOAT family ofputative membrane-bound O-acyltransferases 0.04720.15463&radic&radic&radic S000005968SGF11YPL047WverifiedIntegral subunit of SAGA histone acetyltransferase complex,regulates transcription of a subset ofSAGA-regulated genes, required for the Ubp8passociation with SAGA and for H2Bdeubiquitylation 0.047190.15459&radic&radic&radic S000000030PRP45YAL032CverifiedProtein required for pre-mRNA splicing; associates with thespliceosome and interacts with splicing factorsPrp22p and Prp46p; orthologous to humantranscriptional coactivator SKIP and canactivate transcription of a reporter gene 0.047150.15435&radic&radic S000001128NAM8YHR086WverifiedRNA binding protein, component of the U1 snRNP protein; mutantsare defective in meiotic recombination and information of viable spores, involved in theformation of DSBs through meiosis-specificsplicing of MER2 pre-mRNA 0.047090.15413&radic&radic&radic&radic&radic&radic S000003306SMD1YGR074WverifiedCore Sm protein Sm D1; part of heteroheptameric complex (withSmb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p)that is part of the spliceosomal U1, U2, U4,and U5 snRNPs; homolog of human Sm D1 0.047080.15413&radic&radic&radic&radic S000005372HTZ1YOL012CverifiedHistone variant H2AZ, exchanged for histone H2A in nucleosomesby the SWR1 complex; involved intranscriptional regulation through preventionof the spread of silent heterochromatin 0.047020.15403&radic&radic&radic S000004389AFG2YLR397CverifiedATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexamericcomplex; may be involved in degradation ofaberrant mRNAs 0.047020.15403&radic&radic&radic&radic&radic&radic S000000058GDH3YAL062WverifiedNADP(+)-dependent glutamate dehydrogenase, synthesizesglutamate from ammonia and alpha-ketoglutarate;rate of alpha-ketoglutarate utilization differsfrom Gdh1p; expression regulated by nitrogenand carbon sources 0.047010.15396&radic&radic&radic&radic S000003083SNF4YGL115WverifiedProtein kinase activator found in a complex containing Snf1pand members of the Sip1p/Sip2p/Gal83p family;activates the Snf1p protein kinase; involved inexpression of glucose-repressed genes,sporulation, and peroxisome biogenesis 0.046990.1539&radic&radic S000000404BEM1YBR200WverifiedProtein containing SH3-domains, involved in establishing cellpolarity and morphogenesis; functions as ascaffold protein for complexes that includeCdc24p, Ste5p, Ste20p, and Rsr1p 0.046960.15383&radic&radic&radic S000004029RIC1YLR039CverifiedProtein involved in retrograde transport to the cis-Golginetwork; forms heterodimer with Rgp1p that actsas a GTP exchange factor for Ypt6p; involved intranscription of rRNA and ribosomal proteingenes 0.046940.15374&radic S000003712SWI3YJL176CverifiedSubunit of the SWI/SNF chromatin remodeling complex, whichregulates transcription by remodelingchromosomes; required for transcription of manygenes, including ADH1, ADH2, GAL1, HO, INO1 andSUC2 0.046880.15355&radic&radic&radic S000005498YOL138CUncharacterizedHypothetical protein 0.046910.15355 S000002590CDC1YDR182WverifiedPutative membrane protein of unknown function involved in Mn2+homeostasis; mutants display actin and generalgrowth defects, heterogeneous cell cyclearrests, and pleiotropic defects in cell cycleprogression and organelle distribution 0.046750.15312&radic S000004641CCS1YMR038CverifiedCopper chaperone for superoxide dismutase Sod1p, involved inoxidative stress protection; Met-X-Cys-X2-Cysmotif within the N-terminal portion is involvedin insertion of copper into Sod1p underconditions of copper deprivation 0.046740.15299&radic&radic&radic&radic S000002615UME6YDR207CverifiedKey transcriptional regulator of early meiotic genes, bindsURS1 upstream regulatory sequence, couplesmetabolic responses to nutritional cues withinitiation and progression of meiosis, formscomplex with Ime1p, and also with Sin3p-Rpd3p 0.046670.15287&radic&radic&radic&radic&radic&radic&radic S000001631SDH1YKL148CverifiedFlavoprotein subunit of succinate dehydrogenase (Sdh1p, Sdh2p,Sdh3p, Sdh4p), which couples the oxidation ofsuccinate to the transfer of electrons toubiquinone 0.046580.15257&radic&radic&radic S000005859VMA4YOR332WverifiedSubunit E of the eight-subunit V1 peripheral membrane domain ofthe vacuolar H+-ATPase (V-ATPase), anelectrogenic proton pump found throughout theendomembrane system; required for the V1 domainto assemble onto the vacuolar membrane 0.046590.15257&radic&radic&radic S000004683CTF18YMR078CverifiedSubunit of a complex with Ctf8p that shares some subunits withReplication Factor C and is required for sisterchromatid cohesion; may have overlappingfunctions with Rad24p in the DNA damagereplication checkpoint 0.046540.15241&radic&radic S000001762DYN1YKR054CverifiedCytoplasmic heavy chain dynein, microtubule motor protein,required for anaphase spindle elongation;involved in spindle assembly, chromosomemovement, and spindle orientation during celldivision, targeted to microtubule tips by Pac1p 0.046480.15231&radic&radic S000002186MPS1YDL028CverifiedDual-specificity kinase required for spindle pole body (SPB)duplication and spindle checkpoint function;substrates include SPB proteins Spc42p,Spc110p, and Spc98p, mitotic exit networkprotein Mob1p, and checkpoint protein Mad1p 0.046430.15211 S000001166NDT80YHR124WverifiedMeiosis-specific transcription factor required for exit frompachytene and for full meiotic recombination;activates middle sporulation genes; competeswith Sum1p for binding to promoters containingmiddle sporulation elements (MSE) 0.046430.15211&radic&radic&radic S000003945HIF1YLL022CverifiedNon-essential component of the HAT-B histone acetyltransferasecomplex (Hat1p-Hat2p-Hif1p), localized to thenucleus; has a role in telomeric silencing 0.046420.15204&radic S000003651ASF1YJL115WverifiedNucleosome assembly factor, involved in chromatin assembly anddisassembly, anti-silencing protein that causesderepression of silent loci when overexpressed 0.04630.15173&radic&radic S000006180APM1YPL259CverifiedMu1-like medium subunit of the clathrin-associated proteincomplex (AP-1); binds clathrin; involved inclathrin-dependent Golgi protein sorting 0.046070.15111&radic&radic&radic&radic&radic&radic S000004349RSC2YLR357WverifiedOne of 15 subunits of the 'Remodel the Structure of Chromatin'(RSC) complex; required for expression ofmid-late sporulation-specific genes; involvedin telomere maintenance 0.0460.1509&radic&radic&radic&radic S000003088PRP43YGL120CverifiedRNA helicase in the DEAH-box family, functions in both RNApolymerase I and polymerase II transcriptmetabolism, involved in release of thelariat-intron from the spliceosome 0.045960.15064 S000002828HKR1YDR420WverifiedSerine/threonine rich cell surface protein that contains an EFhand motif; involved in the regulation of cellwall beta-1,3 glucan synthesis and bud siteselection; overexpression confers resistance toHansenula mrakii killer toxin, HM-1 0.045940.15064&radic&radic&radic&radic&radic S000000566MRC1YCL061CverifiedS-phase checkpoint protein found at replication forks, requiredfor DNA replication; also required for Rad53pactivation during DNA replication stress, whereit forms a replication-pausing complex withTof1p and is phosphorylated by Mec1p; proteininvolved in replication checkpoint 0.045880.1504&radic&radic&radic&radic&radic&radic S000005611OST3YOR085WverifiedGamma subunit of the oligosaccharyltransferase complex of theER lumen, which catalyzes asparagine-linkedglycosylation of newly synthesized proteins;Ost3p is important for N-glycosylation of asubset of proteins 0.045830.15028&radic&radic&radic&radic S000000680TUP1YCR084CverifiedGeneral repressor of transcription, forms complex with Cyc8p,involved in the establishment of repressivechromatin structure through interactions withhistones H3 and H4, appears to enhanceexpression of some genes 0.045810.15018&radic S000004434SIR3YLR442CverifiedSilencing protein that interacts with Sir2p and Sir4p, andhistone H3 and H4 tails, to establish atranscriptionally silent chromatin state;required for spreading of silenced chromatin;recruited to chromatin through interaction withRap1p 0.045680.14972 S000003024SDS23YGL056CverifiedOne of two S. cerevisiae homologs (Sds23p and Sds24p) of theSchizosaccharomyces pombe Sds23 protein, whichgenetic studies have implicated inAPC/cyclosome regulation 0.045660.14972&radic&radic&radic&radic&radic&radic&radic S000001411MLP2YIL149CverifiedMyosin-like protein associated with the nuclear envelope,connects the nuclear pore complex with thenuclear interior; involved in the Tel1p pathwaythat controls telomere length 0.045550.14937&radic S000001054VPS29YHR012WverifiedEndosomal protein that is a subunit of the membrane-associatedretromer complex essential forendosome-to-Golgi retrograde transport; forms asubcomplex with Vps35p and Vps26p that selectscargo proteins for endosome-to-Golgi retrieval 0.045510.14928&radic&radic&radic&radic&radic S000002903VPS3YDR495CverifiedCytoplasmic protein required for the sorting and processing ofsoluble vacuolar proteins, acidification of thevacuolar lumen, and assembly of the vacuolarH+-ATPase 0.045450.14904&radic S000002553SWI5YDR146CverifiedTranscription factor that activates transcription of genesexpressed at the M/G1 phase boundary and in G1phase; localization to the nucleus occursduring G1 and appears to be regulated byphosphorylation by Cdc28p kinase 0.04540.14896&radic&radic&radic&radic S000006314RPC40YPR110CverifiedRNA polymerase subunit, common to RNA polymerase I and III 0.04540.14885&radic&radic&radic&radic&radic S000003063VPS45YGL095CverifiedProtein of the Sec1p/Munc-18 family, essential for vacuolarprotein sorting; required for the function ofPep12p and the early endosome/late Golgi SNARETlg2p; essential for fusion of Golgi-derivedvesicles with the prevacuolar compartment 0.045320.14859&radic S000005924ULA1YPL003WverifiedProtein that acts together with Uba3p to activate Rub1p beforeits conjugation to proteins (neddylation),which may play a role in protein degradation 0.045280.14851&radic&radic&radic&radic S000001451SQT1YIR012WverifiedEssential protein involved in a late step of 60S ribosomalsubunit assembly or modification; containsmultiple WD repeats; interacts with Qsr1p in atwo-hybrid assay 0.045260.14844&radic&radic&radic S000003391NSR1YGR159CverifiedNucleolar protein that binds nuclear localization sequences,required for pre-rRNA processing and ribosomebiogenesis 0.045040.14771&radic&radic&radic&radic S000005256RFA2YNL312WverifiedSubunit of heterotrimeric Replication Factor A (RF-A), which isa highly conserved single-stranded DNA bindingprotein involved in DNA replication, repair,and recombination 0.044910.14729&radic&radic&radic&radic&radic S000001695SAC1YKL212WverifiedLipid phosphoinositide phosphatase of the ER and Golgi,involved in protein trafficking and secretion 0.04490.14729&radic&radic&radic S000004050FRS1YLR060WverifiedBeta subunit of cytoplasmic phenylalanyl-tRNA synthetase, formsa tetramer with Frs2p to generate activeenzyme; sequence is evolutionarily distant frommitochondrial phenylalanyl-tRNA synthetase(Msf1p), but substrate binding is similar 0.044870.14725&radic&radic S000001072SLT2YHR030CverifiedSerine/threonine MAP kinase involved in regulating themaintenance of cell wall integrity andprogression through the cell cycle; regulatedby the PKC1-mediated signaling pathway 0.044670.14664&radic&radic&radic S000000861PCL6YER059WverifiedPho85p cyclin of the Pho80p subfamily; forms the major Glc8pkinase together with Pcl7p and Pho85p; involvedin the control of glycogen storage by Pho85p;stabilized by Elongin C binding 0.044530.14609&radic&radic&radic&radic S000005217TOF1YNL273WverifiedSubunit of a replication-pausing checkpoint complex(Tof1p-Mrc1p-Csm3p) that acts at the stalledreplication fork to promote sister chromatidcohesion after DNA damage, facilitating gaprepair of damaged DNA; interacts with the MCMhelicase 0.044540.14609&radic&radic&radic&radic S000003135PMR1YGL167CverifiedHigh affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ andMn2+ transport into Golgi; involved in Ca2+dependent protein sorting and processing;mutations in human homolog ATP2C1 causeacantholytic skin condition Hailey-Haileydisease 0.044470.14595 S000006335NAT3YPR131CverifiedCatalytic subunit of the NatB N-terminal acetyltransferase,which catalyzes acetylation of theamino-terminal methionine residues of allproteins beginning with Met-Asp or Met-Glu andof some proteins beginning with Met-Asn orMet-Met 0.044430.14581&radic&radic&radic&radic&radic S000004778CEP3YMR168CverifiedEssential kinetochore protein, component of the CBF3 complexthat binds the CDEIII region of the centromere;contains an N-terminal Zn2Cys6 type zinc fingerdomain, a C-terminal acidic domain, and aputative coiled coil dimerization domain 0.044370.14567&radic&radic&radic&radic S000005251MCK1YNL307CverifiedProtein serine/threonine/tyrosine (dual-specificity) kinaseinvolved in control of chromosome segregationand in regulating entry into meiosis; relatedto mammalian glycogen synthase kinases of theGSK-3 family 0.044220.14523&radic&radic&radic S000003783LSM8YJR022WverifiedLsm (Like Sm) protein; forms heteroheptameric complex (withLsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p)that is part of spliceosomal U6 snRNP and isalso implicated in processing of pre-tRNA,pre-snoRNA, and pre-rRNA 0.044150.14503&radic&radic&radic S000000001TFC3YAL001CverifiedLargest of six subunits of the RNA polymerase III transcriptioninitiation factor complex (TFIIIC); part of theTauB domain of TFIIIC that binds DNA at theBoxB promoter sites of tRNA and similar genes;cooperates with Tfc6p in DNA binding 0.044120.14489&radic&radic&radic&radic&radic S000000017FUN30YAL019WverifiedProtein whose overexpression affects chromosome stability,potential Cdc28p substrate; homolog of Snf2p;the authentic, non-tagged protein is detectedin highly purified mitochondria inhigh-throughput studies 0.044070.14471&radic&radic S000000122LSM2YBL026WverifiedLsm (Like Sm) protein; part of heteroheptameric complexes(Lsm2p-7p and either Lsm1p or 8p): cytoplasmicLsm1p complex involved in mRNA decay; nuclearLsm8p complex part of U6 snRNP and possiblyinvolved in processing tRNA, snoRNA, and rRNA 0.044090.14471&radic&radic&radic&radic&radic S000000913SWI4YER111CverifiedDNA binding component of the SBF complex (Swi4p-Swi6p), atranscriptional activator that in concert withMBF (Mbp1-Swi6p) regulates late G1-specifictranscription of targets including cyclins andgenes required for DNA synthesis and repair 0.044020.14458&radic&radic S000004093CDC45YLR103CverifiedDNA replication initiation factor; recruited to MCM pre-RCcomplexes at replication origins; promotesrelease of MCM from Mcm10p, recruits elongationmachinery; mutants in human homolog may causevelocardiofacial and DiGeorge syndromes 0.0440.14446&radic&radic&radic&radic&radic&radic S000004695YTA12YMR089CverifiedComponent, with Afg3p, of the mitochondrial inner membranem-AAA protease that mediates degradation ofmisfolded or unassembled proteins and is alsorequired for correct assembly of mitochondrialenzyme complexes 0.043870.14403&radic S000002260CDC2YDL102WverifiedCatalytic subunit of DNA polymerase delta; required forchromosomal DNA replication during mitosis andmeiosis, intragenic recombination, repair ofdouble strand DNA breaks, and DNA replicationduring nucleotide excision repair (NER) 0.043740.1437&radic S000004670SOV1YMR066WuncharacterizedMitochondrial protein of unknown function 0.043670.14354 S000002205SIT4YDL047WverifiedType 2A-related serine-threonine phosphatase that functions inthe G1/S transition of the mitotic cycle;cytoplasmic and nuclear protein that modulatesfunctions mediated by Pkc1p including cell walland actin cytoskeleton organization 0.043660.14348&radic&radic&radic S000001869BST1YFL025CverifiedGPI inositol deacylase of the ER that negatively regulatesCOPII vesicle formation, prevents production ofvesicles with defective subunits, required forproper discrimination between resident ERproteins and Golgi-bound cargo molecules &radic0.04360.14331&radic&radic&radic&radic&radic S000003420BUB1YGR188CverifiedProtein kinase that forms a complex with Mad1p and Bub3p thatis crucial in the checkpoint mechanism requiredto prevent cell cycle progression into anaphasein the presence of spindle damage, associateswith centromere DNA via Skp1p 0.043520.14299&radic&radic S000004189YLR199CUncharacterizedPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR199C is not an essential gene; ORF contains an intron 0.043460.14278&radic&radic&radic&radic&radic&radic S000004439VMA6YLR447CverifiedSubunit D of the five-subunit V0 integral membrane domain ofvacuolar H+-ATPase (V-ATPase), an electrogenicproton pump found in the endomembrane system;stabilizes VO subunits; required for V1 domainassembly on the vacuolar membrane 0.043440.14278 S000005834SLY41YOR307CverifiedProtein involved in ER-to-Golgi transport 0.043350.1424&radic&radic&radic&radic S000003105SEC27YGL137WverifiedEssential beta'-coat protein of the COPI coatomer, involved inER-to-Golgi and Golgi-to-ER transport; containsWD40 domains that mediate cargo selectiveinteractions; 45% sequence identity tomammalian beta'-COP 0.043330.14237 S000000325GRS1YBR121CverifiedCytoplasmic and mitochondrial glycyl-tRNA synthase that ligatesglycine to the cognate anticodon bearing tRNA;transcription termination factor that mayinteract with the 3'-end of pre-mRNA to promote3'-end formation 0.043290.14222&radic&radic S000002587YDR179W-AUncharacterizedHypothetical protein 0.043270.14172&radic&radic&radic&radic S000004614CLU1YMR012WverifiedeIF3 component of unknown function; deletion causes defects inmitochondrial organization but not in growth ortranslation initiation, can rescue cytokinesisand mitochondrial organization defects of theDictyostelium cluA- mutant 0.043260.14172&radic&radic&radic&radic&radic&radic S000005596GYP1YOR070CverifiedCis-golgi GTPase-activating protein (GAP) for the Rab familymembers Ypt1p (in vivo) and for Ypt1p, Sec4p,Ypt7p, and Ypt51p (in vitro); involved invesicle docking and fusion 0.043140.14169&radic&radic&radic&radic&radic&radic S000002868TFB3YDR460WverifiedSubunit of TFIIH and nucleotide excision repair factor 3complexes, involved in transcriptioninitiation, required for nucleotide excisionrepair; ring finger protein similar tomammalian CAK and TFIIH subunit 0.043120.14162&radic S000002703HDA2YDR295CverifiedSubunit of a possibly tetrameric trichostatin A-sensitive classII histone deacetylase complex containing anHda1p homodimer and an Hda2p-Hda3p heterodimer;involved in telomere maintenance 0.04310.14157&radic&radic&radic&radic S000000846ERG28YER044CverifiedEndoplasmic reticulum membrane protein, may facilitateprotein-protein interactions between the Erg26pdehydrogenase and the Erg27p 3-ketoreductaseand/or tether these enzymes to the ER, alsointeracts with Erg6p 0.043080.1415&radic&radic&radic&radic&radic S000002622AHA1YDR214WverifiedCo-chaperone that binds to Hsp82p and activates its ATPaseactivity; similar to Hch1p; expression isregulated by stresses such as heat shock 0.043050.14139&radic&radic&radic&radic S000006328CTR1YPR124WverifiedHigh-affinity copper transporter of the plasma membrane,mediates nearly all copper uptake under lowcopper conditions; transcriptionally induced atlow copper levels and degraded at high copperlevels 0.0430.14124&radic&radic S000002515GSG1YDR108WverifiedSubunit of TRAPP (transport protein particle), a multi-subunitcomplex involved in targeting and/or fusion ofER-to-Golgi transport vesicles with theiracceptor compartment; protein has late meioticrole, following DNA replication 0.042980.141&radic&radic&radic&radic S000002988GET1YGL020CverifiedSubunit of the GET complex; required for the retrieval of HDELproteins from the Golgi to the ER in an ERD2dependent fashion and for normal mitochondrialmorphology and inheritance 0.042980.141 S000001356LYS12YIL094CverifiedHomo-isocitrate dehydrogenase, an NAD-linked mitochondrialenzyme required for the fourth step in thebiosynthesis of lysine, in whichhomo-isocitrate is oxidatively decarboxylatedto alpha-ketoadipate 0.042950.141 S000001635GPM1YKL152CverifiedTetrameric phosphoglycerate mutase, mediates the conversion of3-phosphoglycerate to 2-phosphoglycerate duringglycolysis and the reverse reaction duringgluconeogenesis 0.042860.14074&radic&radic&radic&radic S000001176WSS1YHR134WverifiedSumoylated protein of unknown function, identified based ongenetic interactions with SMT3; UV-sensitivemutant phenotype and genetic interactionssuggest a role in the DNA damage response,processing stalled or collapsed replicationforks 0.042810.14059&radic&radic S000000375SEC66YBR171WverifiedNon-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66pand Sec72p); with Sec61 complex, Kar2p/BiP andLhs1p forms a channel competent forSRP-dependent and post-translationalSRP-independent protein targeting and importinto the ER 0.04270.14025&radic S000004651CSM3YMR048WverifiedProtein required for accurate chromosome segregation duringmeiosis 0.042660.14006&radic S000003023OLE1YGL055WverifiedFatty acid desaturase, required for monounsaturated fatty acidsynthesis and for normal distribution ofmitochondria 0.042630.14&radic&radic&radic&radic S000002600NUP42YDR192CverifiedSubunit of the nuclear pore complex (NPC) that localizesexclusively to the cytoplasmic side; involvedin RNA export, most likely at a terminal step;interacts with Gle1p 0.042560.13979&radic&radic&radic&radic S000002411RAD57YDR004WverifiedProtein that stimulates strand exchange by stabilizing thebinding of Rad51p to single-stranded DNA;involved in the recombinational repair ofdouble-strand breaks in DNA during vegetativegrowth and meiosis; forms heterodimer withRad55p 0.042490.13958&radic&radic S000006131SRP72YPL210CverifiedCore component of the signal recognition particle (SRP)ribonucleoprotein (RNP) complex that functionsin targeting nascent secretory proteins to theendoplasmic reticulum (ER) membrane 0.04240.13916&radic&radic&radic&radic&radic S000003191COG1YGL223CverifiedEssential component of the conserved oligomeric Golgi complex(Cog1p through Cog8p), a cytosolic tetheringcomplex that functions in protein traffickingto mediate fusion of transport vesicles toGolgi compartments 0.042350.13916&radic&radic&radic S000001448MSL1YIR009WverifiedU2B component of U2 snRNP, involved in splicing, binds the U2snRNA stem-loop IV in vitro; does not containthe conserved C-terminal RNA binding domainfound in other family members 0.042330.1391&radic&radic S000002744YDR336WUncharacterizedPutative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene 0.042290.13889&radic&radic&radic&radic&radic S000005853MYO2YOR326WverifiedOne of two type V myosin motors (along with MYO4) involved inactin-based transport of cargos; required forthe polarized delivery of secretory vesicles,the vacuole, late Golgi elements, peroxisomes,and the mitotic spindle 0.042250.13876&radic&radic&radic&radic S000004791SPT21YMR179WverifiedProtein required for normal transcription at several lociincluding HTA2-HTB2 and HHF2-HHT2, but notrequired at the other histone loci;functionally related to Spt10p; involved intelomere maintenance 0.042160.1385&radic&radic&radic&radic&radic&radic S000005564HIR2YOR038CverifiedNon-essential transcriptional corepressor involved in the cellcycle-regulated transcription of histone H2A,H2B, H3, and H4 genes; recruits Swi-Snfcomplexes to histone gene promoters; promotesheterochromatic gene silencing with Asf1p 0.042140.13848&radic&radic&radic&radic S000001149CDC12YHR107CverifiedComponent of the septin ring of the mother-bud neck that isrequired for cytokinesis; septins recruitproteins to the neck and can act as a barrierto diffusion at the me